Affiliation: University of Pittsburgh
- RNAi screening in mammalian cells to identify novel host cell molecules involved in the regulation of viral infectionsCarolyn B Coyne
Department of Microbiology and Molecular Genetics, University of Pittsburgh, Pittsburgh, PA, USA
Methods Mol Biol 721:397-405. 2011..Here, we describe an approach to target mammalian host cell factors involved in regulating viral infections by the use of a genome-scale RNAi library screen...
- Comparative RNAi screening reveals host factors involved in enterovirus infection of polarized endothelial monolayersCarolyn B Coyne
Department of Microbiology and Molecular Genetics, University of Pittsburgh, Pittsburgh, PA 15219, USA
Cell Host Microbe 9:70-82. 2011..These findings highlight the pathways hijacked by enteroviruses for entry and replication in the BBB endothelium, a specialized and clinically relevant cell type for these viruses...
- The distinct roles of JAM-A in reovirus pathogenesisCarolyn B Coyne
Department of Cell Biology and Physiology, University of Pittsburgh, Pittsburgh, PA 15261, USA
Cell Host Microbe 5:3-5. 2009..In this issue of Cell Host & Microbe, Antar et al. (2009) provide striking in vivo evidence that the broadly expressed reovirus receptor JAM-A plays a specific role in reovirus dissemination...
- Coxsackievirus entry across epithelial tight junctions requires occludin and the small GTPases Rab34 and Rab5Carolyn B Coyne
Division of Infectious Diseases, Children s Hospital of Philadelphia, Philadelphia, PA 19104, USA
Cell Host Microbe 2:181-92. 2007..Thus, CVB entry depends on occludin and occurs by a process that combines aspects of caveolar endocytosis with features characteristic of macropinocytosis...
- Poliovirus entry into human brain microvascular cells requires receptor-induced activation of SHP-2Carolyn B Coyne
Division of Infectious Diseases, Children s Hospital of Philadelphia, Philadelphia, PA, USA
EMBO J 26:4016-28. 2007..The results indicate that receptor-induced signals promote virus entry and suggest a role for tyrosine phosphatases in viral pathogenesis...
- Virus-induced Abl and Fyn kinase signals permit coxsackievirus entry through epithelial tight junctionsCarolyn B Coyne
Division of Infectious Diseases, The Children s Hospital of Philadelphia, University of Pennsylvania, PA 19104, USA
Cell 124:119-31. 2006..CVBs thus exploit DAF-mediated signaling pathways to surmount the epithelial barrier...
- Dynamin- and lipid raft-dependent entry of decay-accelerating factor (DAF)-binding and non-DAF-binding coxsackieviruses into nonpolarized cellsKunal P Patel
Division of Infectious Diseases, Children s Hospital of Philadelphia, Philadelphia, Pennsylvania 19104, USA
J Virol 83:11064-77. 2009..These results indicate that CVB3 entry into nonpolarized HeLa cells differs significantly from entry into polarized Caco-2 cells and is not influenced by virus binding to DAF...
- Tight junction proteins claudin-1 and occludin control hepatitis C virus entry and are downregulated during infection to prevent superinfectionShufeng Liu
Department of Infectious Diseases and Microbiology, University of Pittsburgh, Pennsylvania, USA
J Virol 83:2011-4. 2009..The altered TJ protein expression may contribute to the morphological and functional changes observed in HCV-infected hepatocytes...
- The coxsackievirus and adenovirus receptor interacts with the multi-PDZ domain protein-1 (MUPP-1) within the tight junctionCarolyn B Coyne
Division of Infectious Diseases, Children s Hospital of Philadelphia, Pennsylvania 19104, USA
J Biol Chem 279:48079-84. 2004..The inhibition of CAR expression with small interfering RNA inhibited MUPP1 localization to the tight junction. The results indicated that CAR interacts with MUPP1 and is involved in MUPP1 recruitment to the tight junction...
- CAR: a virus receptor within the tight junctionCarolyn B Coyne
Division of Infectious Diseases, 1202 Abramson Research Center, Children s Hospital of Philadelphia, 3615 Civic Center Boulevard, Philadelphia, PA 19104, United States
Adv Drug Deliv Rev 57:869-82. 2005..CAR's biological roles are not well defined, but emerging evidence suggests that it may function during embryonic development and in regulating cell proliferation...
- Correlation of the tight junction-like distribution of Claudin-1 to the cellular tropism of hepatitis C virusWei Yang
Department of Infectious Diseases and Microbiology, University of Pittsburgh, Pittsburgh, PA 15261, USA
J Biol Chem 283:8643-53. 2008..5.1 cells to HCV infection. Our results suggest that the specific localization pattern of CLDN1 may be crucial in the regulation of HCV cellular tropism...
- Role of p38 MAP kinase and transforming growth factor-beta signaling in transepithelial migration of invasive bacterial pathogensChristoph Beisswenger
Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
J Biol Chem 282:28700-8. 2007..pneumoniae or H. influenzae. Our data shows that diverse bacteria utilize common mechanisms, including MAPK and TGF-beta signaling pathways to disrupt epithelial barriers and promote invasion...
- Sar1 assembly regulates membrane constriction and ER exportKimberly R Long
Department of Cell Biology and Physiology, School of Medicine, University of Pittsburgh, Pittsburgh, PA 15261, USA
J Cell Biol 190:115-28. 2010..Sar1 activity was directed to liquid-disordered lipid phases. Thus, lipid-directed and tether-assisted Sar1 organization controls membrane constriction to regulate ER export...
- Regulation of airway tight junctions by proinflammatory cytokinesCarolyn B Coyne
Cystic Fibrosis Pulmonary Research and Treatment Center, The University of North Carolina at Chapel Hill, 27599, USA
Mol Biol Cell 13:3218-34. 2002..These data indicate that the TJ of airway epithelia exposed to chronic inflammation may exhibit parallel changes in the barrier function to both solutes and ions...
- Role of claudin interactions in airway tight junctional permeabilityCarolyn B Coyne
Cystic Fibrosis Pulmonary Research and Treatment Center, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
Am J Physiol Lung Cell Mol Physiol 285:L1166-78. 2003..These suggest that airway TJs are regulated by claudinclaudin interactions that confer the selectivity of the junction...
- Safety and efficiency of modulating paracellular permeability to enhance airway epithelial gene transfer in vivoLarry G Johnson
Cystic Fibrosis Pulmonary Research and Treatment Center, and Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA
Hum Gene Ther 14:729-47. 2003..Adenoviral gene transfer to murine trachea in vivo was enhanced more efficiently by C10 than by C12 or EGTA. Thus, the different toxicities may permit the selection of agents that enhance gene transfer with minimal adverse effects...
- Acute mechanism of medium chain fatty acid-induced enhancement of airway epithelial permeabilityCarolyn B Coyne
Cystic Fibrosis Pulmonary Research and Treatment Center, 7123A Thurston Bowles Bldg, CB No 7248, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7248, USA
J Pharmacol Exp Ther 305:440-50. 2003..These data suggest that C10 and C12 exert their acute effects on airway TJs via a Ca(2+)-independent mechanism of action and may alter junctional permeability via direct effects on the claudin family of TJ proteins...
- Enterovirus entry into polarized endothelial cellsCarolyn B Coyne; Fiscal Year: 2010..The studies in this proposal will identify cellular genes required for mediating enterovirus infection and may lead to the design of cell-type-specific antiviral targets. ..