Carolyn Coyne

Summary

Affiliation: University of Pittsburgh
Country: USA

Publications

  1. pmc The actin cytoskeleton as a barrier to virus infection of polarized epithelial cells
    Elizabeth Delorme-Axford
    Department of Microbiology and Molecular Genetics, University of Pittsburgh, 518 Bridgeside Point II, 450 Technology Drive, Pittsburgh, PA 15219, USA
    Viruses 3:2462-77. 2011
  2. pmc The coxsackievirus B 3C protease cleaves MAVS and TRIF to attenuate host type I interferon and apoptotic signaling
    Amitava Mukherjee
    Department of Cell Biology and Physiology, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
    PLoS Pathog 7:e1001311. 2011
  3. pmc Release of intracellular calcium stores facilitates coxsackievirus entry into polarized endothelial cells
    Rebecca A Bozym
    Department of Microbiology and Molecular Genetics, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
    PLoS Pathog 6:e1001135. 2010
  4. doi request reprint RNAi screening in mammalian cells to identify novel host cell molecules involved in the regulation of viral infections
    Carolyn B Coyne
    Department of Microbiology and Molecular Genetics, University of Pittsburgh, Pittsburgh, PA, USA
    Methods Mol Biol 721:397-405. 2011
  5. pmc Comparative RNAi screening reveals host factors involved in enterovirus infection of polarized endothelial monolayers
    Carolyn B Coyne
    Department of Microbiology and Molecular Genetics, University of Pittsburgh, Pittsburgh, PA 15219, USA
    Cell Host Microbe 9:70-82. 2011
  6. doi request reprint The distinct roles of JAM-A in reovirus pathogenesis
    Carolyn B Coyne
    Department of Cell Biology and Physiology, University of Pittsburgh, Pittsburgh, PA 15261, USA
    Cell Host Microbe 5:3-5. 2009
  7. pmc Coxsackievirus entry across epithelial tight junctions requires occludin and the small GTPases Rab34 and Rab5
    Carolyn B Coyne
    Division of Infectious Diseases, Children s Hospital of Philadelphia, Philadelphia, PA 19104, USA
    Cell Host Microbe 2:181-92. 2007
  8. pmc Poliovirus entry into human brain microvascular cells requires receptor-induced activation of SHP-2
    Carolyn B Coyne
    Division of Infectious Diseases, Children s Hospital of Philadelphia, Philadelphia, PA, USA
    EMBO J 26:4016-28. 2007
  9. pmc Dynamin- and lipid raft-dependent entry of decay-accelerating factor (DAF)-binding and non-DAF-binding coxsackieviruses into nonpolarized cells
    Kunal P Patel
    Division of Infectious Diseases, Children s Hospital of Philadelphia, Philadelphia, Pennsylvania 19104, USA
    J Virol 83:11064-77. 2009
  10. ncbi request reprint Virus-induced Abl and Fyn kinase signals permit coxsackievirus entry through epithelial tight junctions
    Carolyn B Coyne
    Division of Infectious Diseases, The Children s Hospital of Philadelphia, University of Pennsylvania, PA 19104, USA
    Cell 124:119-31. 2006

Research Grants

Collaborators

Detail Information

Publications21

  1. pmc The actin cytoskeleton as a barrier to virus infection of polarized epithelial cells
    Elizabeth Delorme-Axford
    Department of Microbiology and Molecular Genetics, University of Pittsburgh, 518 Bridgeside Point II, 450 Technology Drive, Pittsburgh, PA 15219, USA
    Viruses 3:2462-77. 2011
    ....
  2. pmc The coxsackievirus B 3C protease cleaves MAVS and TRIF to attenuate host type I interferon and apoptotic signaling
    Amitava Mukherjee
    Department of Cell Biology and Physiology, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
    PLoS Pathog 7:e1001311. 2011
    ..Taken together, these data show that CVB3 has evolved a mechanism to suppress host antiviral signal propagation by directly cleaving two key adaptor molecules associated with innate immune recognition...
  3. pmc Release of intracellular calcium stores facilitates coxsackievirus entry into polarized endothelial cells
    Rebecca A Bozym
    Department of Microbiology and Molecular Genetics, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
    PLoS Pathog 6:e1001135. 2010
    ..These data point to a specific role for calcium signaling in CVB entry into polarized endothelial monolayers and highlight the unique signaling mechanisms used by these viruses to cross endothelial barriers...
  4. doi request reprint RNAi screening in mammalian cells to identify novel host cell molecules involved in the regulation of viral infections
    Carolyn B Coyne
    Department of Microbiology and Molecular Genetics, University of Pittsburgh, Pittsburgh, PA, USA
    Methods Mol Biol 721:397-405. 2011
    ..Here, we describe an approach to target mammalian host cell factors involved in regulating viral infections by the use of a genome-scale RNAi library screen...
  5. pmc Comparative RNAi screening reveals host factors involved in enterovirus infection of polarized endothelial monolayers
    Carolyn B Coyne
    Department of Microbiology and Molecular Genetics, University of Pittsburgh, Pittsburgh, PA 15219, USA
    Cell Host Microbe 9:70-82. 2011
    ..These findings highlight the pathways hijacked by enteroviruses for entry and replication in the BBB endothelium, a specialized and clinically relevant cell type for these viruses...
  6. doi request reprint The distinct roles of JAM-A in reovirus pathogenesis
    Carolyn B Coyne
    Department of Cell Biology and Physiology, University of Pittsburgh, Pittsburgh, PA 15261, USA
    Cell Host Microbe 5:3-5. 2009
    ..In this issue of Cell Host & Microbe, Antar et al. (2009) provide striking in vivo evidence that the broadly expressed reovirus receptor JAM-A plays a specific role in reovirus dissemination...
  7. pmc Coxsackievirus entry across epithelial tight junctions requires occludin and the small GTPases Rab34 and Rab5
    Carolyn B Coyne
    Division of Infectious Diseases, Children s Hospital of Philadelphia, Philadelphia, PA 19104, USA
    Cell Host Microbe 2:181-92. 2007
    ..Thus, CVB entry depends on occludin and occurs by a process that combines aspects of caveolar endocytosis with features characteristic of macropinocytosis...
  8. pmc Poliovirus entry into human brain microvascular cells requires receptor-induced activation of SHP-2
    Carolyn B Coyne
    Division of Infectious Diseases, Children s Hospital of Philadelphia, Philadelphia, PA, USA
    EMBO J 26:4016-28. 2007
    ..The results indicate that receptor-induced signals promote virus entry and suggest a role for tyrosine phosphatases in viral pathogenesis...
  9. pmc Dynamin- and lipid raft-dependent entry of decay-accelerating factor (DAF)-binding and non-DAF-binding coxsackieviruses into nonpolarized cells
    Kunal P Patel
    Division of Infectious Diseases, Children s Hospital of Philadelphia, Philadelphia, Pennsylvania 19104, USA
    J Virol 83:11064-77. 2009
    ..These results indicate that CVB3 entry into nonpolarized HeLa cells differs significantly from entry into polarized Caco-2 cells and is not influenced by virus binding to DAF...
  10. ncbi request reprint Virus-induced Abl and Fyn kinase signals permit coxsackievirus entry through epithelial tight junctions
    Carolyn B Coyne
    Division of Infectious Diseases, The Children s Hospital of Philadelphia, University of Pennsylvania, PA 19104, USA
    Cell 124:119-31. 2006
    ..CVBs thus exploit DAF-mediated signaling pathways to surmount the epithelial barrier...
  11. pmc COPI activity coupled with fatty acid biosynthesis is required for viral replication
    Sara Cherry
    University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, United States of America
    PLoS Pathog 2:e102. 2006
    ..Additionally, because these pathways are also limiting in flies and in human cells infected with the related RNA virus poliovirus, they may represent novel targets for antiviral therapies...
  12. pmc Tight junction proteins claudin-1 and occludin control hepatitis C virus entry and are downregulated during infection to prevent superinfection
    Shufeng Liu
    Department of Infectious Diseases and Microbiology, University of Pittsburgh, Pennsylvania, USA
    J Virol 83:2011-4. 2009
    ..The altered TJ protein expression may contribute to the morphological and functional changes observed in HCV-infected hepatocytes...
  13. ncbi request reprint The coxsackievirus and adenovirus receptor interacts with the multi-PDZ domain protein-1 (MUPP-1) within the tight junction
    Carolyn B Coyne
    Division of Infectious Diseases, Children s Hospital of Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 279:48079-84. 2004
    ..The inhibition of CAR expression with small interfering RNA inhibited MUPP1 localization to the tight junction. The results indicated that CAR interacts with MUPP1 and is involved in MUPP1 recruitment to the tight junction...
  14. pmc Correlation of the tight junction-like distribution of Claudin-1 to the cellular tropism of hepatitis C virus
    Wei Yang
    Department of Infectious Diseases and Microbiology, University of Pittsburgh, Pittsburgh, PA 15261, USA
    J Biol Chem 283:8643-53. 2008
    ..5.1 cells to HCV infection. Our results suggest that the specific localization pattern of CLDN1 may be crucial in the regulation of HCV cellular tropism...
  15. ncbi request reprint CAR: a virus receptor within the tight junction
    Carolyn B Coyne
    Division of Infectious Diseases, 1202 Abramson Research Center, Children s Hospital of Philadelphia, 3615 Civic Center Boulevard, Philadelphia, PA 19104, United States
    Adv Drug Deliv Rev 57:869-82. 2005
    ..CAR's biological roles are not well defined, but emerging evidence suggests that it may function during embryonic development and in regulating cell proliferation...
  16. pmc Sar1 assembly regulates membrane constriction and ER export
    Kimberly R Long
    Department of Cell Biology and Physiology, School of Medicine, University of Pittsburgh, Pittsburgh, PA 15261, USA
    J Cell Biol 190:115-28. 2010
    ..Sar1 activity was directed to liquid-disordered lipid phases. Thus, lipid-directed and tether-assisted Sar1 organization controls membrane constriction to regulate ER export...
  17. ncbi request reprint Role of p38 MAP kinase and transforming growth factor-beta signaling in transepithelial migration of invasive bacterial pathogens
    Christoph Beisswenger
    Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 282:28700-8. 2007
    ..pneumoniae or H. influenzae. Our data shows that diverse bacteria utilize common mechanisms, including MAPK and TGF-beta signaling pathways to disrupt epithelial barriers and promote invasion...
  18. pmc Regulation of airway tight junctions by proinflammatory cytokines
    Carolyn B Coyne
    Cystic Fibrosis Pulmonary Research and Treatment Center, The University of North Carolina at Chapel Hill, 27599, USA
    Mol Biol Cell 13:3218-34. 2002
    ..These data indicate that the TJ of airway epithelia exposed to chronic inflammation may exhibit parallel changes in the barrier function to both solutes and ions...
  19. ncbi request reprint Role of claudin interactions in airway tight junctional permeability
    Carolyn B Coyne
    Cystic Fibrosis Pulmonary Research and Treatment Center, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Am J Physiol Lung Cell Mol Physiol 285:L1166-78. 2003
    ..These suggest that airway TJs are regulated by claudinclaudin interactions that confer the selectivity of the junction...
  20. ncbi request reprint Safety and efficiency of modulating paracellular permeability to enhance airway epithelial gene transfer in vivo
    Larry G Johnson
    Cystic Fibrosis Pulmonary Research and Treatment Center, and Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA
    Hum Gene Ther 14:729-47. 2003
    ..Adenoviral gene transfer to murine trachea in vivo was enhanced more efficiently by C10 than by C12 or EGTA. Thus, the different toxicities may permit the selection of agents that enhance gene transfer with minimal adverse effects...
  21. ncbi request reprint Acute mechanism of medium chain fatty acid-induced enhancement of airway epithelial permeability
    Carolyn B Coyne
    Cystic Fibrosis Pulmonary Research and Treatment Center, 7123A Thurston Bowles Bldg, CB No 7248, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7248, USA
    J Pharmacol Exp Ther 305:440-50. 2003
    ..These data suggest that C10 and C12 exert their acute effects on airway TJs via a Ca(2+)-independent mechanism of action and may alter junctional permeability via direct effects on the claudin family of TJ proteins...

Research Grants2

  1. Enterovirus entry into polarized endothelial cells
    Carolyn B Coyne; Fiscal Year: 2010
    ..The studies in this proposal will identify cellular genes required for mediating enterovirus infection and may lead to the design of cell-type-specific antiviral targets. ..
  2. Enterovirus entry into polarized endothelial cells
    Carolyn Coyne; Fiscal Year: 2009
    ..The studies in this proposal will identify cellular genes required for mediating enterovirus infection and may lead to the design of cell-type-specific antiviral targets. ..