S R Coughlin

Summary

Affiliation: University of California
Country: USA

Publications

  1. ncbi request reprint Protease-activated receptor 3 is a second thrombin receptor in humans
    H Ishihara
    Cardiovascular Research Institute, University of California, San Francisco 94143 0130, USA
    Nature 386:502-6. 1997
  2. ncbi request reprint Role of the thrombin receptor's cytoplasmic tail in intracellular trafficking. Distinct determinants for agonist-triggered versus tonic internalization and intracellular localization
    M J Shapiro
    Cardiovascular Research Institute, University of California, San Francisco, California 94143 0130, USA
    J Biol Chem 271:32874-80. 1996
  3. ncbi request reprint A dual thrombin receptor system for platelet activation
    M L Kahn
    Cardiovascular Research Institute, Department of Medicine, University of California, San Francisco 94143 0130, USA
    Nature 394:690-4. 1998
  4. pmc Protease-activated receptors 1 and 4 mediate activation of human platelets by thrombin
    M L Kahn
    Cardiovascular Research Institute and Daiichi Research Center, University of California San Francisco, San Francisco, California 94143 0130, USA
    J Clin Invest 103:879-87. 1999
  5. ncbi request reprint Gene and locus structure and chromosomal localization of the protease-activated receptor gene family
    M L Kahn
    Cardiovascular Research Institute, University of California, San Francisco, California 94143 0130, USA
    J Biol Chem 273:23290-6. 1998
  6. ncbi request reprint Role of thrombin signalling in platelets in haemostasis and thrombosis
    G R Sambrano
    Cardiovascular Research Institute, University of California, 513 Parnassus Avenue, San Francisco, California 94143 0130, USA
    Nature 413:74-8. 2001
  7. ncbi request reprint A role for thrombin receptor signaling in endothelial cells during embryonic development
    C T Griffin
    Cardiovascular Research Institute, University of California at San Francisco UCSF, San Francisco, California 94143
    Science 293:1666-70. 2001
  8. ncbi request reprint Impaired hemostasis and protection against thrombosis in protease-activated receptor 4-deficient mice is due to lack of thrombin signaling in platelets
    J R Hamilton
    Cardiovascular Research Institute, University of California, San Francisco, USA
    J Thromb Haemost 2:1429-35. 2004
  9. ncbi request reprint PAR3 is a cofactor for PAR4 activation by thrombin
    M Nakanishi-Matsui
    Cardiovascular Research Institute and Daiichi Research Center, University of California, San Francisco 94143 0130, USA
    Nature 404:609-13. 2000
  10. pmc Disparate temporal expression of the prothrombin and thrombin receptor genes during mouse development
    S J Soifer
    Cardiovascular Research Institute, University of California, San Francisco 94143
    Am J Pathol 144:60-9. 1994

Collaborators

Detail Information

Publications37

  1. ncbi request reprint Protease-activated receptor 3 is a second thrombin receptor in humans
    H Ishihara
    Cardiovascular Research Institute, University of California, San Francisco 94143 0130, USA
    Nature 386:502-6. 1997
    ..PAR3 provides a new tool for understanding thrombin signalling and a possible target for therapeutics designed selectively to block thrombotic, inflammatory and proliferative responses to thrombin...
  2. ncbi request reprint Role of the thrombin receptor's cytoplasmic tail in intracellular trafficking. Distinct determinants for agonist-triggered versus tonic internalization and intracellular localization
    M J Shapiro
    Cardiovascular Research Institute, University of California, San Francisco, California 94143 0130, USA
    J Biol Chem 271:32874-80. 1996
    ..They further suggest that maintenance of the intracellular pool of naive thrombin receptors requires tonic receptor internalization...
  3. ncbi request reprint A dual thrombin receptor system for platelet activation
    M L Kahn
    Cardiovascular Research Institute, Department of Medicine, University of California, San Francisco 94143 0130, USA
    Nature 394:690-4. 1998
    ..The identification of a two-receptor system for platelet activation by thrombin has important implications for the development of antithrombotic therapies...
  4. pmc Protease-activated receptors 1 and 4 mediate activation of human platelets by thrombin
    M L Kahn
    Cardiovascular Research Institute and Daiichi Research Center, University of California San Francisco, San Francisco, California 94143 0130, USA
    J Clin Invest 103:879-87. 1999
    ..These observations suggest that PAR1 and PAR4 account for most, if not all, thrombin signaling in platelets and that antagonists that block these receptors might be useful antithrombotic agents...
  5. ncbi request reprint Gene and locus structure and chromosomal localization of the protease-activated receptor gene family
    M L Kahn
    Cardiovascular Research Institute, University of California, San Francisco, California 94143 0130, USA
    J Biol Chem 273:23290-6. 1998
    ..A comparison of the structures of the PAR amino acid sequences, gene structures, locus organization, and chromosomal locations suggests a working model for PAR gene evolution...
  6. ncbi request reprint Role of thrombin signalling in platelets in haemostasis and thrombosis
    G R Sambrano
    Cardiovascular Research Institute, University of California, 513 Parnassus Avenue, San Francisco, California 94143 0130, USA
    Nature 413:74-8. 2001
    ..Thus platelet activation by thrombin is necessary for normal haemostasis and may be an important target in the treatment of thrombosis...
  7. ncbi request reprint A role for thrombin receptor signaling in endothelial cells during embryonic development
    C T Griffin
    Cardiovascular Research Institute, University of California at San Francisco UCSF, San Francisco, California 94143
    Science 293:1666-70. 2001
    ....
  8. ncbi request reprint Impaired hemostasis and protection against thrombosis in protease-activated receptor 4-deficient mice is due to lack of thrombin signaling in platelets
    J R Hamilton
    Cardiovascular Research Institute, University of California, San Francisco, USA
    J Thromb Haemost 2:1429-35. 2004
    ....
  9. ncbi request reprint PAR3 is a cofactor for PAR4 activation by thrombin
    M Nakanishi-Matsui
    Cardiovascular Research Institute and Daiichi Research Center, University of California, San Francisco 94143 0130, USA
    Nature 404:609-13. 2000
    ..This establishes a paradigm for cofactor-assisted PAR activation and for a G-protein-coupled receptor's acting as an accessory molecule to present ligand to another receptor...
  10. pmc Disparate temporal expression of the prothrombin and thrombin receptor genes during mouse development
    S J Soifer
    Cardiovascular Research Institute, University of California, San Francisco 94143
    Am J Pathol 144:60-9. 1994
    ..The finding of robust thrombin receptor expression before prothrombin mRNA was detected raises the question of whether other proteases or peptide ligands can activate the thrombin receptor...
  11. ncbi request reprint Protease-activated receptors in the cardiovascular system
    S R Coughlin
    Cardiovascular Research Institute, Department of Medicine and Department of Cellular and Molecular Pharmacology, University of California, San Francisco, California 94143, USA
    Cold Spring Harb Symp Quant Biol 67:197-208. 2002
  12. ncbi request reprint The cloned thrombin receptor is necessary and sufficient for activation of mitogen-activated protein kinase and mitogenesis in mouse lung fibroblasts. Loss of responses in fibroblasts from receptor knockout mice
    J Trejo
    Cardiovascular Research Institute, University of California, San Francisco 94143 0524, USA
    J Biol Chem 271:21536-41. 1996
    ..Thus in mouse lung fibroblasts, one thrombin receptor utilizes two pathways for MAP kinase activation: one is protein kinase C- and c-Raf dependent, and a second is Gi-dependent and c-Raf-independent...
  13. ncbi request reprint Protease-activated receptors in hemostasis, thrombosis and vascular biology
    S R Coughlin
    Cardiovascular Research Institute, Department of Medicine, University of California, San Francisco, CA 94143 0130, USA
    J Thromb Haemost 3:1800-14. 2005
    ....
  14. pmc Conserved structure and adjacent location of the thrombin receptor and protease-activated receptor 2 genes define a protease-activated receptor gene cluster
    M Kahn
    Department of Medicine, University of California, San Francisco 94143 0524, USA
    Mol Med 2:349-57. 1996
    ....
  15. ncbi request reprint Cellular localization of membrane-type serine protease 1 and identification of protease-activated receptor-2 and single-chain urokinase-type plasminogen activator as substrates
    T Takeuchi
    Department of Pharmaceutical Chemistry and Biochemistry and Biophysics, Cardiovascular Research Institute, University of California, San Francisco, California 94143, USA
    J Biol Chem 275:26333-42. 2000
    ..The membrane localization of MT-SP1 and its affinity for these key extracellular substrates suggests a role of the proteolytic activity in regulatory events...
  16. ncbi request reprint Identification of cDNAs encoding two G protein-coupled receptors for lysosphingolipids
    S An
    Department of Medicine, University of California, San Francisco 94143, USA
    FEBS Lett 417:279-82. 1997
    ..H218 and Edg3 expressed in Xenopus oocytes conferred responsiveness to S1P and dihydro-S1P in agonist-triggered 45Ca2+ efflux. Therefore, H218 and Edg3 are functional receptors for S1P and perhaps other closely related lysosphingolipids...
  17. ncbi request reprint Cathepsin G activates protease-activated receptor-4 in human platelets
    G R Sambrano
    Cardiovascular Research Institute, University of California, San Francisco, San Francisco, California 94143 0130, USA
    J Biol Chem 275:6819-23. 2000
    ..These data show that PAR4 mediates platelet responses to cathepsin G and support the hypothesis that cathepsin G might mediate neutrophil-platelet interactions at sites of vascular injury or inflammation...
  18. ncbi request reprint A mammalian homolog of fission yeast Cdc5 regulates G2 progression and mitotic entry
    H S Bernstein
    Cardiovascular Research Institute, University of California, San Francisco, California 94143, USA
    J Biol Chem 273:4666-71. 1998
    ..Thus, hCdc5 is the first transcriptional regulator shown to affect G2 progression and mitotic entry in mammalian cells...
  19. pmc How the protease thrombin talks to cells
    S R Coughlin
    Cardiovascular Research Institute, Department of Medicine, University of California, San Francisco, CA 94143 0130, USA
    Proc Natl Acad Sci U S A 96:11023-7. 1999
    ..PAR2 is activated by trypsin and by trypsin-like proteases but not by thrombin. Recent studies with knockout mice, receptor-activating peptides, and blocking antibodies are beginning to define the role of these receptors in vivo...
  20. ncbi request reprint Protease-activated receptors in vascular biology
    S R Coughlin
    Department of Medicine, University of California, San Francisco 94143 0130, USA
    Thromb Haemost 86:298-307. 2001
    ..Our current understanding of the role of PARs in platelet and endothelial cell activation and their potential importance in normal and disease states is discussed...
  21. pmc Molecular cloning, expression and potential functions of the human proteinase-activated receptor-2
    S K Bohm
    Department of Surgery, University of California, San Francisco, 94143 0660, USA
    Biochem J 314:1009-16. 1996
    ..Thus PAR-2 may serve as a trypsin sensor in the gut. Its expression by cells and tissues not normally exposed to pancreatic trypsin suggests that other proteases could serve as physiological activators...
  22. pmc Mice lacking the thrombin receptor, PAR1, have normal skin wound healing
    A J Connolly
    Department of Pathology, University of California, San Francisco 94143 0130, USA
    Am J Pathol 151:1199-204. 1997
    ..We conclude that PAR1 is not necessary for normal skin wound healing in mice...
  23. pmc Thrombin receptor expression in normal and atherosclerotic human arteries
    N A Nelken
    Cardiovascular Research Institute, University of California, San Francisco 94143
    J Clin Invest 90:1614-21. 1992
    ..These results establish thrombin receptor activation as a candidate for contributing to sclerotic and inflammatory processes in the human vasculature, such as those that occur in atherosclerosis and restenosis...
  24. ncbi request reprint Pombe Cdc5-related protein. A putative human transcription factor implicated in mitogen-activated signaling
    H S Bernstein
    Cardiovascular Research Institute, University of California, San Francisco, California 94143, USA
    J Biol Chem 272:5833-7. 1997
    ..This movement correlated temporally with an increase in PCDC5RP phosphorylation. Thus, PCDC5RP is a presumed transcription factor that appears to transduce cytoplasmic signals to the nucleus upon serum stimulation...
  25. ncbi request reprint Role of the thrombin receptor in development and evidence for a second receptor
    A J Connolly
    Cardiovascular Research Institute, University of California, San Francisco, 94143 0524, USA
    Nature 381:516-9. 1996
    ..Moreover, a second platelet thrombin receptor exists, and different thrombin receptors have tissue-specific roles. This may allow development of therapeutics that will selectively block thrombin's different cellular actions...
  26. ncbi request reprint Specificity of the thrombin receptor for agonist peptide is defined by its extracellular surface
    R E Gerszten
    Cardiovascular Research Institute, University of California, San Francisco 94143 0524
    Nature 368:648-51. 1994
    ..Our results indicate that agonist interaction with extracellular domains is important for thrombin receptor activation...
  27. ncbi request reprint A common PDGF receptor is activated by homodimeric A and B forms of PDGF
    J A Escobedo
    Department of Medicine, University of California, San Francisco
    Science 240:1532-4. 1988
    ....
  28. pmc Characterization of a functional thrombin receptor. Issues and opportunities
    S R Coughlin
    Cardiovascular Research Institute, University of California, San Francisco 94143
    J Clin Invest 89:351-5. 1992
  29. pmc Molecular cloning and functional expression of two monocyte chemoattractant protein 1 receptors reveals alternative splicing of the carboxyl-terminal tails
    I F Charo
    Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94141 9100
    Proc Natl Acad Sci U S A 91:2752-6. 1994
    ..The identification of the MCP-1 receptor and cloning of two distinct isoforms provide powerful tools for understanding the specificity and signaling mechanisms of this important chemokine...
  30. ncbi request reprint Intracellular targeting and trafficking of thrombin receptors. A novel mechanism for resensitization of a G protein-coupled receptor
    L Hein
    Division of Cardiovascular Medicine, Stanford University Medical School, California 94305
    J Biol Chem 269:27719-26. 1994
    ..These observations reveal a novel trafficking mechanism for resensitizing the thrombin receptor as opposed to the internalization/recycling pathway of other G protein-coupled receptors...
  31. ncbi request reprint cDNA cloning and expression of platelet p24/CD9. Evidence for a new family of multiple membrane-spanning proteins
    F Lanza
    COR Therapeutics, Inc, South San Francisco, California 94080
    J Biol Chem 266:10638-45. 1991
    ..These data indicate the presence of a new family of surface antigens that may function in cellular activation and differentiation...
  32. ncbi request reprint Molecular cloning of a functional thrombin receptor reveals a novel proteolytic mechanism of receptor activation
    T K Vu
    Department of Medicine, University of California, San Francisco 94143 0524
    Cell 64:1057-68. 1991
    ..These data reveal a novel signaling mechanism in which thrombin cleaves its receptor's amino-terminal extension to create a new receptor amino terminus that functions as a tethered ligand and activates the receptor...
  33. ncbi request reprint Thrombin receptors on human platelets. Initial localization and subsequent redistribution during platelet activation
    M Molino
    Department of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 272:6011-7. 1997
    ..This difference underlies the ability of endothelial cells to recover responsiveness to thrombin rapidly while platelets do not, despite the presence on both of the same receptor for thrombin...
  34. ncbi request reprint Crystallographic structures of thrombin complexed with thrombin receptor peptides: existence of expected and novel binding modes
    I I Mathews
    Department of Chemistry, Michigan State University, East Lansing 48824 1322
    Biochemistry 33:3266-79. 1994
    ..The physiological role for this unexpected intermolecular binding mode, if any, remains to be identified.(ABSTRACT TRUNCATED AT 400 WORDS)..
  35. pmc Protease-activated receptor 1 mediates thrombin-dependent, cell-mediated renal inflammation in crescentic glomerulonephritis
    M A Cunningham
    Centre for Inflammatory Diseases, Monash University Department of Medicine, Monash Medical Centre, 3168 Victoria, Australia
    J Exp Med 191:455-62. 2000
    ..These results indicate that activation of PAR-1 by thrombin or TRAP amplifies crescentic GN. Thus, in addition to its procoagulant role, thrombin has proinflammatory, PAR-1-dependent effects that augment inflammatory renal injury...
  36. ncbi request reprint Delayed onset of inflammation in protease-activated receptor-2-deficient mice
    J R Lindner
    Cardiovascular Division and Department of Biomedical Engineering, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    J Immunol 165:6504-10. 2000
    ..These results indicate that activation of PAR2 produces microvascular inflammation by rapid induction of P-selectin-mediated leukocyte rolling. In the absence of PAR2, the onset of inflammation is delayed...
  37. ncbi request reprint Glycoprotein V-deficient platelets have undiminished thrombin responsiveness and Do not exhibit a Bernard-Soulier phenotype
    M L Kahn
    Department of Medicine, University of Pennsylvania, Philadelphia, PA, USA
    Blood 94:4112-21. 1999
    ..Whether redundancy accounts for the lack of phenotype of GPV-deficiency or whether GPV serves subtle or as yet unprobed functions in platelets or other cells remains to be determined...