Research Topics
Genomes and Genes
Species | S R CoughlinSummaryAffiliation: University of California Country: USA Publications
| Collaborators
|
Detail Information
Publications
Protease-activated receptor 3 is a second thrombin receptor in humansH Ishihara
Cardiovascular Research Institute, University of California, San Francisco 94143 0130, USA
Nature 386:502-6. 1997..PAR3 provides a new tool for understanding thrombin signalling and a possible target for therapeutics designed selectively to block thrombotic, inflammatory and proliferative responses to thrombin...- Role of the thrombin receptor's cytoplasmic tail in intracellular trafficking. Distinct determinants for agonist-triggered versus tonic internalization and intracellular localizationM J Shapiro
Cardiovascular Research Institute, University of California, San Francisco, California 94143 0130, USA
J Biol Chem 271:32874-80. 1996..They further suggest that maintenance of the intracellular pool of naive thrombin receptors requires tonic receptor internalization... - A dual thrombin receptor system for platelet activationM L Kahn
Cardiovascular Research Institute, Department of Medicine, University of California, San Francisco 94143 0130, USA
Nature 394:690-4. 1998..The identification of a two-receptor system for platelet activation by thrombin has important implications for the development of antithrombotic therapies... 
Protease-activated receptors 1 and 4 mediate activation of human platelets by thrombinM L Kahn
Cardiovascular Research Institute and Daiichi Research Center, University of California San Francisco, San Francisco, California 94143 0130, USA
J Clin Invest 103:879-87. 1999..These observations suggest that PAR1 and PAR4 account for most, if not all, thrombin signaling in platelets and that antagonists that block these receptors might be useful antithrombotic agents...- Gene and locus structure and chromosomal localization of the protease-activated receptor gene familyM L Kahn
Cardiovascular Research Institute, University of California, San Francisco, California 94143 0130, USA
J Biol Chem 273:23290-6. 1998..A comparison of the structures of the PAR amino acid sequences, gene structures, locus organization, and chromosomal locations suggests a working model for PAR gene evolution... - Role of thrombin signalling in platelets in haemostasis and thrombosisG R Sambrano
Cardiovascular Research Institute, University of California, 513 Parnassus Avenue, San Francisco, California 94143 0130, USA
Nature 413:74-8. 2001..Thus platelet activation by thrombin is necessary for normal haemostasis and may be an important target in the treatment of thrombosis... - A role for thrombin receptor signaling in endothelial cells during embryonic developmentC T Griffin
Cardiovascular Research Institute, University of California at San Francisco UCSF, San Francisco, California 94143
Science 293:1666-70. 2001.... - Impaired hemostasis and protection against thrombosis in protease-activated receptor 4-deficient mice is due to lack of thrombin signaling in plateletsJ R Hamilton
Cardiovascular Research Institute, University of California, San Francisco, USA
J Thromb Haemost 2:1429-35. 2004.... - PAR3 is a cofactor for PAR4 activation by thrombinM Nakanishi-Matsui
Cardiovascular Research Institute and Daiichi Research Center, University of California, San Francisco 94143 0130, USA
Nature 404:609-13. 2000..This establishes a paradigm for cofactor-assisted PAR activation and for a G-protein-coupled receptor's acting as an accessory molecule to present ligand to another receptor... - Disparate temporal expression of the prothrombin and thrombin receptor genes during mouse developmentS J Soifer
Cardiovascular Research Institute, University of California, San Francisco 94143
Am J Pathol 144:60-9. 1994..The finding of robust thrombin receptor expression before prothrombin mRNA was detected raises the question of whether other proteases or peptide ligands can activate the thrombin receptor... - Protease-activated receptors in the cardiovascular systemS R Coughlin
Cardiovascular Research Institute, Department of Medicine and Department of Cellular and Molecular Pharmacology, University of California, San Francisco, California 94143, USA
Cold Spring Harb Symp Quant Biol 67:197-208. 2002 - The cloned thrombin receptor is necessary and sufficient for activation of mitogen-activated protein kinase and mitogenesis in mouse lung fibroblasts. Loss of responses in fibroblasts from receptor knockout miceJ Trejo
Cardiovascular Research Institute, University of California, San Francisco 94143 0524, USA
J Biol Chem 271:21536-41. 1996..Thus in mouse lung fibroblasts, one thrombin receptor utilizes two pathways for MAP kinase activation: one is protein kinase C- and c-Raf dependent, and a second is Gi-dependent and c-Raf-independent... - Protease-activated receptors in hemostasis, thrombosis and vascular biologyS R Coughlin
Cardiovascular Research Institute, Department of Medicine, University of California, San Francisco, CA 94143 0130, USA
J Thromb Haemost 3:1800-14. 2005.... - Conserved structure and adjacent location of the thrombin receptor and protease-activated receptor 2 genes define a protease-activated receptor gene clusterM Kahn
Department of Medicine, University of California, San Francisco 94143 0524, USA
Mol Med 2:349-57. 1996.... - Cellular localization of membrane-type serine protease 1 and identification of protease-activated receptor-2 and single-chain urokinase-type plasminogen activator as substratesT Takeuchi
Department of Pharmaceutical Chemistry and Biochemistry and Biophysics, Cardiovascular Research Institute, University of California, San Francisco, California 94143, USA
J Biol Chem 275:26333-42. 2000..The membrane localization of MT-SP1 and its affinity for these key extracellular substrates suggests a role of the proteolytic activity in regulatory events... - Identification of cDNAs encoding two G protein-coupled receptors for lysosphingolipidsS An
Department of Medicine, University of California, San Francisco 94143, USA
FEBS Lett 417:279-82. 1997..H218 and Edg3 expressed in Xenopus oocytes conferred responsiveness to S1P and dihydro-S1P in agonist-triggered 45Ca2+ efflux. Therefore, H218 and Edg3 are functional receptors for S1P and perhaps other closely related lysosphingolipids... - Cathepsin G activates protease-activated receptor-4 in human plateletsG R Sambrano
Cardiovascular Research Institute, University of California, San Francisco, San Francisco, California 94143 0130, USA
J Biol Chem 275:6819-23. 2000..These data show that PAR4 mediates platelet responses to cathepsin G and support the hypothesis that cathepsin G might mediate neutrophil-platelet interactions at sites of vascular injury or inflammation... - A mammalian homolog of fission yeast Cdc5 regulates G2 progression and mitotic entryH S Bernstein
Cardiovascular Research Institute, University of California, San Francisco, California 94143, USA
J Biol Chem 273:4666-71. 1998..Thus, hCdc5 is the first transcriptional regulator shown to affect G2 progression and mitotic entry in mammalian cells... - How the protease thrombin talks to cellsS R Coughlin
Cardiovascular Research Institute, Department of Medicine, University of California, San Francisco, CA 94143 0130, USA
Proc Natl Acad Sci U S A 96:11023-7. 1999..PAR2 is activated by trypsin and by trypsin-like proteases but not by thrombin. Recent studies with knockout mice, receptor-activating peptides, and blocking antibodies are beginning to define the role of these receptors in vivo... - Protease-activated receptors in vascular biologyS R Coughlin
Department of Medicine, University of California, San Francisco 94143 0130, USA
Thromb Haemost 86:298-307. 2001..Our current understanding of the role of PARs in platelet and endothelial cell activation and their potential importance in normal and disease states is discussed... - Molecular cloning, expression and potential functions of the human proteinase-activated receptor-2S K Bohm
Department of Surgery, University of California, San Francisco, 94143 0660, USA
Biochem J 314:1009-16. 1996..Thus PAR-2 may serve as a trypsin sensor in the gut. Its expression by cells and tissues not normally exposed to pancreatic trypsin suggests that other proteases could serve as physiological activators... - Mice lacking the thrombin receptor, PAR1, have normal skin wound healingA J Connolly
Department of Pathology, University of California, San Francisco 94143 0130, USA
Am J Pathol 151:1199-204. 1997..We conclude that PAR1 is not necessary for normal skin wound healing in mice... - Thrombin receptor expression in normal and atherosclerotic human arteriesN A Nelken
Cardiovascular Research Institute, University of California, San Francisco 94143
J Clin Invest 90:1614-21. 1992..These results establish thrombin receptor activation as a candidate for contributing to sclerotic and inflammatory processes in the human vasculature, such as those that occur in atherosclerosis and restenosis... - Pombe Cdc5-related protein. A putative human transcription factor implicated in mitogen-activated signalingH S Bernstein
Cardiovascular Research Institute, University of California, San Francisco, California 94143, USA
J Biol Chem 272:5833-7. 1997..This movement correlated temporally with an increase in PCDC5RP phosphorylation. Thus, PCDC5RP is a presumed transcription factor that appears to transduce cytoplasmic signals to the nucleus upon serum stimulation... - Role of the thrombin receptor in development and evidence for a second receptorA J Connolly
Cardiovascular Research Institute, University of California, San Francisco, 94143 0524, USA
Nature 381:516-9. 1996..Moreover, a second platelet thrombin receptor exists, and different thrombin receptors have tissue-specific roles. This may allow development of therapeutics that will selectively block thrombin's different cellular actions... - Specificity of the thrombin receptor for agonist peptide is defined by its extracellular surfaceR E Gerszten
Cardiovascular Research Institute, University of California, San Francisco 94143 0524
Nature 368:648-51. 1994..Our results indicate that agonist interaction with extracellular domains is important for thrombin receptor activation... - A common PDGF receptor is activated by homodimeric A and B forms of PDGFJ A Escobedo
Department of Medicine, University of California, San Francisco
Science 240:1532-4. 1988.... - Characterization of a functional thrombin receptor. Issues and opportunitiesS R Coughlin
Cardiovascular Research Institute, University of California, San Francisco 94143
J Clin Invest 89:351-5. 1992 - Molecular cloning and functional expression of two monocyte chemoattractant protein 1 receptors reveals alternative splicing of the carboxyl-terminal tailsI F Charo
Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94141 9100
Proc Natl Acad Sci U S A 91:2752-6. 1994..The identification of the MCP-1 receptor and cloning of two distinct isoforms provide powerful tools for understanding the specificity and signaling mechanisms of this important chemokine... - Intracellular targeting and trafficking of thrombin receptors. A novel mechanism for resensitization of a G protein-coupled receptorL Hein
Division of Cardiovascular Medicine, Stanford University Medical School, California 94305
J Biol Chem 269:27719-26. 1994..These observations reveal a novel trafficking mechanism for resensitizing the thrombin receptor as opposed to the internalization/recycling pathway of other G protein-coupled receptors... - cDNA cloning and expression of platelet p24/CD9. Evidence for a new family of multiple membrane-spanning proteinsF Lanza
COR Therapeutics, Inc, South San Francisco, California 94080
J Biol Chem 266:10638-45. 1991..These data indicate the presence of a new family of surface antigens that may function in cellular activation and differentiation... - Molecular cloning of a functional thrombin receptor reveals a novel proteolytic mechanism of receptor activationT K Vu
Department of Medicine, University of California, San Francisco 94143 0524
Cell 64:1057-68. 1991..These data reveal a novel signaling mechanism in which thrombin cleaves its receptor's amino-terminal extension to create a new receptor amino terminus that functions as a tethered ligand and activates the receptor... - Thrombin receptors on human platelets. Initial localization and subsequent redistribution during platelet activationM Molino
Department of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
J Biol Chem 272:6011-7. 1997..This difference underlies the ability of endothelial cells to recover responsiveness to thrombin rapidly while platelets do not, despite the presence on both of the same receptor for thrombin... - Crystallographic structures of thrombin complexed with thrombin receptor peptides: existence of expected and novel binding modesI I Mathews
Department of Chemistry, Michigan State University, East Lansing 48824 1322
Biochemistry 33:3266-79. 1994..The physiological role for this unexpected intermolecular binding mode, if any, remains to be identified.(ABSTRACT TRUNCATED AT 400 WORDS).. - Protease-activated receptor 1 mediates thrombin-dependent, cell-mediated renal inflammation in crescentic glomerulonephritisM A Cunningham
Centre for Inflammatory Diseases, Monash University Department of Medicine, Monash Medical Centre, 3168 Victoria, Australia
J Exp Med 191:455-62. 2000..These results indicate that activation of PAR-1 by thrombin or TRAP amplifies crescentic GN. Thus, in addition to its procoagulant role, thrombin has proinflammatory, PAR-1-dependent effects that augment inflammatory renal injury... - Delayed onset of inflammation in protease-activated receptor-2-deficient miceJ R Lindner
Cardiovascular Division and Department of Biomedical Engineering, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
J Immunol 165:6504-10. 2000..These results indicate that activation of PAR2 produces microvascular inflammation by rapid induction of P-selectin-mediated leukocyte rolling. In the absence of PAR2, the onset of inflammation is delayed... - Glycoprotein V-deficient platelets have undiminished thrombin responsiveness and Do not exhibit a Bernard-Soulier phenotypeM L Kahn
Department of Medicine, University of Pennsylvania, Philadelphia, PA, USA
Blood 94:4112-21. 1999..Whether redundancy accounts for the lack of phenotype of GPV-deficiency or whether GPV serves subtle or as yet unprobed functions in platelets or other cells remains to be determined...