S D Copley

Summary

Affiliation: University of Colorado
Country: USA

Publications

  1. ncbi request reprint Enzymes with extra talents: moonlighting functions and catalytic promiscuity
    Shelley D Copley
    Department of Molecular, Cellular, and Developmental Biology and Cooperative Institute for Research in Environmental Sciences, University of Colorado at Boulder Campus, Box 216, Boulder, CO 80309 0216, USA
    Curr Opin Chem Biol 7:265-72. 2003
  2. doi request reprint A trade-off between catalytic power and substrate inhibition in TCHQ dehalogenase
    Joseph R Warner
    Department of Molecular, Cellular, and Developmental Biology and Cooperative Institute for Research in Environmental Sciences, University of Colorado at Boulder, Boulder, Colorado 80309, USA
    Biochemistry 47:3258-65. 2008
  3. ncbi request reprint Identification and localization of a stable sulfenic acid in peroxide-treated tetrachlorohydroquinone dehalogenase using electrospray mass spectrometry
    W S Willett
    Department of Chemistry and Biochemistry and Cooperative Institute for Research in Environmental Sciences, University of Colorado at Boulder, Boulder, CO 80309, USA
    Chem Biol 3:851-7. 1996
  4. pmc Lateral gene transfer and parallel evolution in the history of glutathione biosynthesis genes
    Shelley D Copley
    Department of Molecular, Cellular, and Developmental Biology and the Cooperative Institute for Research in Environmental Sciences, University of Colorado at Boulder, Campus Box 216, Boulder, CO 80309, USA
    Genome Biol 3:research0025. 2002
  5. ncbi request reprint Evolution of a metabolic pathway for degradation of a toxic xenobiotic: the patchwork approach
    S D Copley
    Dept of Chemistry and Biochemistry and Cooperative Institute for Research in Environmental Studies, University of Colorado at Boulder, Boulder, CO 80309, USA
    Trends Biochem Sci 25:261-5. 2000
  6. ncbi request reprint Microbial dehalogenases: enzymes recruited to convert xenobiotic substrates
    S D Copley
    University of Colorado at Boulder, Department of Chemistry and Biochemistry 80309, USA
    Curr Opin Chem Biol 2:613-7. 1998
  7. pmc Evolution of efficient pathways for degradation of anthropogenic chemicals
    Shelley D Copley
    Department of Molecular, Cellular and Developmental Biology and Cooperative Institute for Research in Environmental Sciences, University of Colorado at Boulder, Boulder, Colorado, USA
    Nat Chem Biol 5:559-66. 2009
  8. ncbi request reprint Divergence of function in the thioredoxin fold suprafamily: evidence for evolution of peroxiredoxins from a thioredoxin-like ancestor
    Shelley D Copley
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309, USA
    Biochemistry 43:13981-95. 2004
  9. ncbi request reprint The origin of the RNA world: co-evolution of genes and metabolism
    Shelley D Copley
    University of Colorado at Boulder, CIRES, Campus Box 216, Boulder, CO 80309, USA
    Bioorg Chem 35:430-43. 2007
  10. ncbi request reprint Mechanism of the severe inhibition of tetrachlorohydroquinone dehalogenase by its aromatic substrates
    Joseph R Warner
    Department of Chemistry and Biochemistry, University of Colorado at Boulder, Boulder, Colorado 80309, USA
    Biochemistry 46:4438-47. 2007

Collaborators

Detail Information

Publications19

  1. ncbi request reprint Enzymes with extra talents: moonlighting functions and catalytic promiscuity
    Shelley D Copley
    Department of Molecular, Cellular, and Developmental Biology and Cooperative Institute for Research in Environmental Sciences, University of Colorado at Boulder Campus, Box 216, Boulder, CO 80309 0216, USA
    Curr Opin Chem Biol 7:265-72. 2003
    ..New insights into catalytic promiscuity are provided by studies of aminoglycoside kinase (3') type IIIa, tetrachlorohydroquinone dehalogenase, and aldolase antibody 38C2...
  2. doi request reprint A trade-off between catalytic power and substrate inhibition in TCHQ dehalogenase
    Joseph R Warner
    Department of Molecular, Cellular, and Developmental Biology and Cooperative Institute for Research in Environmental Sciences, University of Colorado at Boulder, Boulder, Colorado 80309, USA
    Biochemistry 47:3258-65. 2008
    ....
  3. ncbi request reprint Identification and localization of a stable sulfenic acid in peroxide-treated tetrachlorohydroquinone dehalogenase using electrospray mass spectrometry
    W S Willett
    Department of Chemistry and Biochemistry and Cooperative Institute for Research in Environmental Sciences, University of Colorado at Boulder, Boulder, CO 80309, USA
    Chem Biol 3:851-7. 1996
    ..The damage is reversible by treatment with dithiothreitol. Possible types of oxidative damage include an inappropriate disulfide bond, a cysteine sulfenic acid, or a methionine sulfoxide...
  4. pmc Lateral gene transfer and parallel evolution in the history of glutathione biosynthesis genes
    Shelley D Copley
    Department of Molecular, Cellular, and Developmental Biology and the Cooperative Institute for Research in Environmental Sciences, University of Colorado at Boulder, Campus Box 216, Boulder, CO 80309, USA
    Genome Biol 3:research0025. 2002
    ..To evaluate this, we have used bioinformatics to analyze sequences of the biosynthetic enzymes gamma-glutamylcysteine ligase and glutathione synthetase...
  5. ncbi request reprint Evolution of a metabolic pathway for degradation of a toxic xenobiotic: the patchwork approach
    S D Copley
    Dept of Chemistry and Biochemistry and Cooperative Institute for Research in Environmental Studies, University of Colorado at Boulder, Boulder, CO 80309, USA
    Trends Biochem Sci 25:261-5. 2000
    ..This apparently recently assembled pathway does not function very well: pentachlorophenol hydroxylase is quite slow, and tetrachlorohydroquinone dehalogenase is subject to severe substrate inhibition...
  6. ncbi request reprint Microbial dehalogenases: enzymes recruited to convert xenobiotic substrates
    S D Copley
    University of Colorado at Boulder, Department of Chemistry and Biochemistry 80309, USA
    Curr Opin Chem Biol 2:613-7. 1998
    ....
  7. pmc Evolution of efficient pathways for degradation of anthropogenic chemicals
    Shelley D Copley
    Department of Molecular, Cellular and Developmental Biology and Cooperative Institute for Research in Environmental Sciences, University of Colorado at Boulder, Boulder, Colorado, USA
    Nat Chem Biol 5:559-66. 2009
    ....
  8. ncbi request reprint Divergence of function in the thioredoxin fold suprafamily: evidence for evolution of peroxiredoxins from a thioredoxin-like ancestor
    Shelley D Copley
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309, USA
    Biochemistry 43:13981-95. 2004
    ....
  9. ncbi request reprint The origin of the RNA world: co-evolution of genes and metabolism
    Shelley D Copley
    University of Colorado at Boulder, CIRES, Campus Box 216, Boulder, CO 80309, USA
    Bioorg Chem 35:430-43. 2007
    ....
  10. ncbi request reprint Mechanism of the severe inhibition of tetrachlorohydroquinone dehalogenase by its aromatic substrates
    Joseph R Warner
    Department of Chemistry and Biochemistry, University of Colorado at Boulder, Boulder, Colorado 80309, USA
    Biochemistry 46:4438-47. 2007
    ..In addition, dissociation of the GSSG product is inhibited by TriCHQ. The thiol-disulfide exchange reaction is the rate-limiting step in the reductive dehalogenation reaction under physiological conditions...
  11. pmc A compromise required by gene sharing enables survival: Implications for evolution of new enzyme activities
    Sean Yu McLoughlin
    Department of Molecular, Cellular, and Developmental Biology and Cooperative Institute of Environmental Sciences, University of Colorado, Boulder, CO 80309, USA
    Proc Natl Acad Sci U S A 105:13497-502. 2008
    ..Notably, overexpression does not require a mutation in the regulatory region of the protein; amino acid limitation attributable to the poor catalytic abilities of E383A ProA causes a physiological response that results in overexpression...
  12. pmc MotifCluster: an interactive online tool for clustering and visualizing sequences using shared motifs
    Micah Hamady
    Department of Computer Science, University of Colorado, Boulder, CO 80309, USA
    Genome Biol 9:R128. 2008
    ..We demonstrate MotifCluster's accuracy using gold-standard protein superfamilies; using recommended settings, families were assigned to the correct superfamilies with 0.17% false positive and no false negative assignments...
  13. ncbi request reprint Why metabolic enzymes are essential or nonessential for growth of Escherichia coli K12 on glucose
    Juhan Kim
    Cooperative Institute for Research in Environmental Sciences and Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309, USA
    Biochemistry 46:12501-11. 2007
    ..coli to survive in a variety of habitats and therefore to have a metabolism that allows optimal exploitation of varying environmental resources and synthesis of small molecules when they cannot be obtained from the environment...
  14. ncbi request reprint A mechanistic investigation of the thiol-disulfide exchange step in the reductive dehalogenation catalyzed by tetrachlorohydroquinone dehalogenase
    Joseph R Warner
    Department of Molecular, Cellular, and Developmental Biology and Cooperative Institute for Research in Environmental Sciences, University of Colorado at Boulder, Campus Box 216, Boulder, Colorado 80309 0216, USA
    Biochemistry 44:10360-8. 2005
    ..Remarkably, single-turnover kinetic studies show that the enzyme catalyzes this step by approximately 10000-fold. This high reactivity requires an as yet unidentified protonated group in the active site...
  15. pmc A mechanism for the association of amino acids with their codons and the origin of the genetic code
    Shelley D Copley
    Cooperative Institute for Research in Environmental Sciences, Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309, USA
    Proc Natl Acad Sci U S A 102:4442-7. 2005
    ....
  16. pmc Genome shuffling improves degradation of the anthropogenic pesticide pentachlorophenol by Sphingobium chlorophenolicum ATCC 39723
    MingHua Dai
    Department of Molecular, Cellular, and Developmental Biology and Cooperative Institute for Research in Environmental Sciences, University of Colorado at Boulder, Boulder, Colorado 80309, USA
    Appl Environ Microbiol 70:2391-7. 2004
    ....
  17. ncbi request reprint Characterization of the initial steps in the reductive dehalogenation catalyzed by tetrachlorohydroquinone dehalogenase
    Philip M Kiefer
    Department of Molecular, Cellular, and Developmental Biology and Cooperative Institute for Research in Environmental Sciences, Campus Box 216, University of Colorado at Boulder, Boulder, Colorado 80309, USA
    Biochemistry 41:1315-22. 2002
    ..Possible similarities between this mechanism and the mechanism for isomerization of maleylacetoacetate and maleylpyruvate are discussed...
  18. ncbi request reprint The reaction catalyzed by tetrachlorohydroquinone dehalogenase does not involve nucleophilic aromatic substitution
    Philip M Kiefer
    Department of Molecular, Cellular, and Developmental Biology and Cooperative Institute for Research in Environmental Sciences, Campus Box 216, University of Colorado at Boulder, Boulder, Colorado 80309, USA
    Biochemistry 41:1308-14. 2002
    ..Third, substantial normal solvent kinetic isotope effects on k(cat) and k(cat)/K(M,TriCHQ) are observed. Nonenzymatic and enzymatic nucleophilic S(N)Ar reactions typically show inverse solvent kinetic isotope effects...
  19. pmc Multiple turnovers of the nicotino-enzyme PdxB require α-keto acids as cosubstrates
    Johannes Rudolph
    Department of Chemistry and Biochemistry, University of Colorado at Boulder, Boulder, CO 80309, USA
    Biochemistry 49:9249-55. 2010
    ..7 × 10(6) M⁻¹s⁻¹. Additionally, we have characterized the stereochemistry of α-ketoglutarate reduction by showing that d-2-HGA, but not l-2-HGA, is a competitive inhibitor vs 4PE and a noncompetitive inhibitor vs α-ketoglutarate...