Davi K C Cooper

Summary

Affiliation: University of Pittsburgh
Country: USA

Publications

  1. ncbi request reprint Alpha1,3-galactosyltransferase gene-knockout pigs for xenotransplantation: where do we go from here?
    David K C Cooper
    the Thomas E Starzl Transplantation Institute, Department of Surgery, University of Pittsburgh, Pittsburgh, PA, USA
    Transplantation 84:1-7. 2007
  2. pmc Attempted depletion of passenger leukocytes by irradiation in pigs
    Hao Chih Tai
    Thomas E Starzl Transplantation Institute, University of Pittsburgh Medical Center, Pittsburgh, PA 15261, USA
    J Transplant 2011:928759. 2011
  3. pmc Genetically-engineered pig-to-baboon liver xenotransplantation: histopathology of xenografts and native organs
    Burcin Ekser
    Thomas E Starzl Transplantation Institute, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 7:e29720. 2012
  4. pmc Clinical lung xenotransplantation--what donor genetic modifications may be necessary?
    David K C Cooper
    Thomas E Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, PA 15261, USA
    Xenotransplantation 19:144-58. 2012
  5. pmc The surgical anatomy of experimental and clinical thoracic organ transplantation
    David K C Cooper
    Transplantation Biology Research Center, Department of Surgery, Massachusetts General Hospital Harvard Medical School, Boston, Massachusetts, USA
    Tex Heart Inst J 31:61-8. 2004
  6. ncbi request reprint Recent advances in pig-to-human organ and cell transplantation
    David K C Cooper
    University of Pittsburgh, Thomas E Starzl Transplantation Institute, Starzl Biomedical Science Tower, W1543, 200 Lothrop Street, Pittsburgh, PA 15261, USA
    Expert Opin Biol Ther 8:1-4. 2008
  7. ncbi request reprint Statement on allogeneic and xenogeneic transplantation by Fifty-Seventh World Health Assembly, 22 May 2004
    D K C Cooper
    UPMC MUH, Thomas E Starzl Transplantation Institute, Room N761 3459, Fifth Avenue, Pittsburgh, PA 15213, USA
    Xenotransplantation 11:390. 2004
  8. ncbi request reprint A review of studies relating to thyroid hormone therapy in brain-dead organ donors
    Davi K C Cooper
    Thomas E Starzl Transplantation Institute, Department of Surgery, University of Pittsburgh, Pittsburgh, PA, USA
    Front Biosci (Landmark Ed) 14:3750-70. 2009
  9. doi request reprint Pig heart xenotransplantation as a bridge to allotransplantation
    David K C Cooper
    Thomas E Starzl Transplantation Institute, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania 15261, USA
    J Heart Lung Transplant 29:838-40. 2010
  10. doi request reprint The immense potential of xenotransplantation in surgery
    David K C Cooper
    Thomas E Starzl Transplantation Institute, University of Pittsburgh Medical Center, BST W1543, 200 Lothrop Street, Pittsburgh, PA 15261, USA
    Int J Surg 9:122-9. 2011

Research Grants

Detail Information

Publications91

  1. ncbi request reprint Alpha1,3-galactosyltransferase gene-knockout pigs for xenotransplantation: where do we go from here?
    David K C Cooper
    the Thomas E Starzl Transplantation Institute, Department of Surgery, University of Pittsburgh, Pittsburgh, PA, USA
    Transplantation 84:1-7. 2007
    ..The ultimate goal of induction of T-cell tolerance may be possible only if these hurdles in the coagulation system and innate immunity can be overcome...
  2. pmc Attempted depletion of passenger leukocytes by irradiation in pigs
    Hao Chih Tai
    Thomas E Starzl Transplantation Institute, University of Pittsburgh Medical Center, Pittsburgh, PA 15261, USA
    J Transplant 2011:928759. 2011
    ....
  3. pmc Genetically-engineered pig-to-baboon liver xenotransplantation: histopathology of xenografts and native organs
    Burcin Ekser
    Thomas E Starzl Transplantation Institute, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 7:e29720. 2012
    ..Immunohistochemistry showed minimal deposition of IgM, and almost absence of IgG, C3, C4d, C5b-9, and of a cellular infiltrate, suggesting that neither antibody- nor cell-mediated rejection played a major role...
  4. pmc Clinical lung xenotransplantation--what donor genetic modifications may be necessary?
    David K C Cooper
    Thomas E Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, PA 15261, USA
    Xenotransplantation 19:144-58. 2012
    ..The optimistic view would be that if experimental pig lung xenotransplantation could be successfully managed, it is likely that clinical application of this and all other forms of xenotransplantation would become more feasible...
  5. pmc The surgical anatomy of experimental and clinical thoracic organ transplantation
    David K C Cooper
    Transplantation Biology Research Center, Department of Surgery, Massachusetts General Hospital Harvard Medical School, Boston, Massachusetts, USA
    Tex Heart Inst J 31:61-8. 2004
    ..Aspects of the surgical techniques of these various experimental and clinical procedures are discussed...
  6. ncbi request reprint Recent advances in pig-to-human organ and cell transplantation
    David K C Cooper
    University of Pittsburgh, Thomas E Starzl Transplantation Institute, Starzl Biomedical Science Tower, W1543, 200 Lothrop Street, Pittsburgh, PA 15261, USA
    Expert Opin Biol Ther 8:1-4. 2008
    ..Further genetic modification of the organ-source pig to overcome these barriers is being undertaken...
  7. ncbi request reprint Statement on allogeneic and xenogeneic transplantation by Fifty-Seventh World Health Assembly, 22 May 2004
    D K C Cooper
    UPMC MUH, Thomas E Starzl Transplantation Institute, Room N761 3459, Fifth Avenue, Pittsburgh, PA 15213, USA
    Xenotransplantation 11:390. 2004
  8. ncbi request reprint A review of studies relating to thyroid hormone therapy in brain-dead organ donors
    Davi K C Cooper
    Thomas E Starzl Transplantation Institute, Department of Surgery, University of Pittsburgh, Pittsburgh, PA, USA
    Front Biosci (Landmark Ed) 14:3750-70. 2009
    ..The administration of T3 at the time of discontinuation of cardiopulmonary bypass reverses this state. In patients undergoing heart transplantation, T3 therapy to both donor and recipient is beneficial...
  9. doi request reprint Pig heart xenotransplantation as a bridge to allotransplantation
    David K C Cooper
    Thomas E Starzl Transplantation Institute, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania 15261, USA
    J Heart Lung Transplant 29:838-40. 2010
    ..The experimental results that would be required to warrant consideration of a clinical trial and the patients in whom bridging with a xenograft might be considered preferable to a mechanical device are discussed...
  10. doi request reprint The immense potential of xenotransplantation in surgery
    David K C Cooper
    Thomas E Starzl Transplantation Institute, University of Pittsburgh Medical Center, BST W1543, 200 Lothrop Street, Pittsburgh, PA 15261, USA
    Int J Surg 9:122-9. 2011
    ....
  11. ncbi request reprint Draft reports for public comment from the US Secretary's Advisory Committee on Xenotransplantation
    D K C Cooper
    Xenotransplantation 12:255-7. 2005
  12. doi request reprint Outwitting evolution
    David K C Cooper
    Thomas E Starzl Transplantation Institute, University of Pittsburgh Medical Center, Pittsburgh, PA 15261, USA
    Xenotransplantation 17:171-80. 2010
    ....
  13. doi request reprint Genetically engineered pigs as a source for clinical red blood cell transfusion
    David K C Cooper
    Department of Surgery, Thomas E Starzl Transplantation Institute, University of Pittsburgh Medical Center, 200 Lothrop Street, Pittsburgh, PA 15261, USA
    Clin Lab Med 30:365-80. 2010
    ....
  14. doi request reprint The International Xenotransplantation Association consensus statement on conditions for undertaking clinical trials of porcine islet products in type 1 diabetes--chapter 4: Pre-clinical efficacy and complication data required to justify a clinical trial
    David K C Cooper
    Thomas E Starzl Transplantation Institute, University of Pittsburgh Medical Center, Pittsburgh, PA 15261, USA
    Xenotransplantation 16:229-38. 2009
    ....
  15. ncbi request reprint Extended coagulation profiles of healthy baboons and of baboons rejecting GT-KO pig heart grafts
    Mohamed Ezzelarab
    Thomas E Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, PA 15213, USA
    Xenotransplantation 13:522-8. 2006
    ..Studies have investigated the coagulation profile in baboons post-xenotransplantation (XTx), but an extended coagulation profile of healthy baboons pre-XTx has not been reported...
  16. ncbi request reprint Rapid loss of intraportally transplanted islets: an overview of pathophysiology and preventive strategies
    Dirk J van der Windt
    Department of Pediatrics, Division of Immunogenetics, Children s Hospital of Pittsburgh, Pittsburgh, PA, USA
    Xenotransplantation 14:288-97. 2007
    ..The future of clinical islet transplantation will, at least in part, depend on the success of efforts made to reduce rapid graft loss, and thus allow islet transplantation to become a more efficient therapy by the use of single donors...
  17. ncbi request reprint Antibodies directed to pig non-Gal antigens in naïve and sensitized baboons
    Mohamed Ezzelarab
    Thomas E Starzl Transplantation Institute, University of Pittsburgh Medical Center, Pittsburgh, PA 15261, USA
    Xenotransplantation 13:400-7. 2006
    ..As pigs homozygous for alpha1,3-galactosyltransferase gene-knockout (GT-KO) are available, primate antibodies to pig non-Gal antigens can be studied...
  18. ncbi request reprint Xenogeneic thymokidney and thymic tissue transplantation in a pig-to-baboon model: I. Evidence for pig-specific T-cell unresponsiveness
    Rolf N Barth
    Transplantation Biology Research Center, Massachusetts General Hospital Harvard Medical School, Boston, MA, USA
    Transplantation 75:1615-24. 2003
    ..We tested a combination of these approaches in a clinically relevant pig-to-primate model of xenotransplantation...
  19. ncbi request reprint Late onset of development of natural anti-nonGal antibodies in infant humans and baboons: implications for xenotransplantation in infants
    Pleunie P M Rood
    Thomas E Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, PA 15261, USA
    Transpl Int 20:1050-8. 2007
    ..Anti-blood group antibodies were not detected before 16 weeks of age. Our data suggest GT-KO pig organ/cell transplants could be carried out in early infancy in the absence of preformed cytotoxic anti-nonGalalpha1,3Gal antibodies...
  20. ncbi request reprint Elicited antibodies in baboons exposed to tissues from alpha1,3-galactosyltransferase gene-knockout pigs
    Yau Lin Tseng
    Transplantation Biology Research Center, Massachusetts General Hospital Harvard Medical School, Boston, USA
    Transplantation 81:1058-62. 2006
    ..This study investigates anti-nonGal antibodies (Abs) in baboons after alpha1,3-galactosyltransferase gene-knockout (GalT-KO) pig heart transplantation (Tx)...
  21. doi request reprint Insulin secretion and glucose metabolism in alpha 1,3-galactosyltransferase knock-out pigs compared to wild-type pigs
    Anna Casu
    Division of Immunogenetics, Department of Pediatrics, Children s Hospital of Pittsburgh, Pittsburgh, PA, USA
    Xenotransplantation 17:131-9. 2010
    ..To overcome the immunological obstacle of antibody-mediated rejection, pigs homozygous for alpha1,3-galactosyltransferase gene knock-out (GT-KO) have been produced. The effect of this mutation on glucose metabolism is unknown...
  22. ncbi request reprint alpha1,3-Galactosyltransferase gene-knockout miniature swine produce natural cytotoxic anti-Gal antibodies
    Frank J M F Dor
    Transplantation Biology Research Center, Massachusetts General Hospital Harvard Medical School, Boston, MA 02129, USA
    Transplantation 78:15-20. 2004
    ..The expression of galactose alpha 1,3 galactose (Gal) in pigs has proved a barrier to xenotransplantation. Miniature swine lacking Gal (Gal pigs) have been produced by nuclear transfer/embryo transfer...
  23. pmc Suppressive efficacy and proliferative capacity of human regulatory T cells in allogeneic and xenogeneic responses
    Yih Jyh Lin
    Thomas E Starzl Transplantation Institute, Department of Surgery, University of Pittsburgh Medical Center, Pittsburgh, PA 15261, USA
    Transplantation 86:1452-62. 2008
    ..An understanding of the mechanisms that suppress the human anti-pig cellular response is key for xenotransplantation. We have compared the ability of human regulatory T cells (Tregs) to suppress xenogeneic and allogeneic responses in vitro...
  24. pmc Thrombotic microangiopathy associated with humoral rejection of cardiac xenografts from alpha1,3-galactosyltransferase gene-knockout pigs in baboons
    Akira Shimizu
    Transplantation Biology Research Center, Massachusetts General Hospital Harvard Medical School, Boston, MA 02129, USA
    Am J Pathol 172:1471-81. 2008
    ....
  25. ncbi request reprint Reduction of early graft loss after intraportal porcine islet transplantation in monkeys
    Pleunie P M Rood
    Division of Immunogenetics, Department of Pediatrics, Children s Hospital of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Transplantation 83:202-10. 2007
    ..After intraportal infusion of porcine islets in primates, many islets are lost through what has been termed the instant blood-mediated inflammatory reaction (IBMIR). We report on our experience with IBMIR...
  26. ncbi request reprint Acute vascular rejection of xenografts: roles of natural and elicited xenoreactive antibodies in activation of vascular endothelial cells and induction of procoagulant activity
    Bernd Gollackner
    Transplantation Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02215, USA
    Transplantation 77:1735-41. 2004
    ..The aim of this study was to determine associations between humoral responses to the xenograft and the induction of AVR, coagulopathy, or both...
  27. ncbi request reprint Heart transplantation in baboons using alpha1,3-galactosyltransferase gene-knockout pigs as donors: initial experience
    Kenji Kuwaki
    Transplantation Biology Research Center, Massachusetts General Hospital Harvard Medical School, MGH East, 13th Street, Boston, Massachusetts 02129, USA
    Nat Med 11:29-31. 2005
    ..There were no infectious complications directly related to the immunosuppressive regimen. The transplantation of hearts from GalT-KO pigs increased graft survival over previous studies...
  28. ncbi request reprint Clinical xenotransplantion--how close are we?
    David K C Cooper
    Transplantation Biology Research Center, Massachusetts General Hospital, Building 149 9019, 13th Street, Boston, MA 02129, USA
    Lancet 362:557-9. 2003
    ....
  29. pmc Current status of xenotransplantation and prospects for clinical application
    Richard N Pierson
    Division of Cardiac Surgery, Department of Surgery, University of Maryland, Baltimore VAMC, Baltimore, MD 21201, USA
    Xenotransplantation 16:263-80. 2009
    ..This review offers a comprehensive overview of known mechanisms of xenograft injury, a contemporary assessment of preclinical progress and residual barriers, and our opinions regarding where breakthroughs are likely to occur...
  30. doi request reprint Genetically engineered pig red blood cells for clinical transfusion: initial in vitro studies
    Cassandra Long
    Thomas E Starzl Transplantation Institute, University of Pittsburgh Medical Center, Starzl Biomedical Science Tower, W1540, 200 Lothrop Street, Pittsburgh, PA 15261, USA
    Transfusion 49:2418-29. 2009
    ..This study investigated in vitro the compatibility of genetically engineered pig RBCs (pRBCs) with the human innate immune response...
  31. ncbi request reprint The choice of anatomical site for islet transplantation
    Dirk J van der Windt
    Division of Immunogenetics, Department of Pediatrics, Children s Hospital of Pittsburgh, Pittsburgh, PA, USA
    Cell Transplant 17:1005-14. 2008
    ..Eventually, the liver will most likely be replaced by a site that allows long-term survival of islets from a single donor to reverse type 1 diabetes...
  32. doi request reprint Xenotransplantation of solid organs in the pig-to-primate model
    Burcin Ekser
    Thomas E Starzl Transplantation Institute, University of Pittsburgh Medical Center, Pittsburgh, PA, USA
    Transpl Immunol 21:87-92. 2009
    ....
  33. pmc Overcoming the barriers to xenotransplantation: prospects for the future
    Burcin Ekser
    Thomas E Starzl Transplantation Institute, University of Pittsburgh Medical Center, Pittsburgh, PA 15261, USA
    Expert Rev Clin Immunol 6:219-30. 2010
    ..Further advances are required to overcome the remaining barriers...
  34. doi request reprint The innate immune response and activation of coagulation in alpha1,3-galactosyltransferase gene-knockout xenograft recipients
    Mohamed Ezzelarab
    Thomas E Starzl Transplantation Institute, Department of Surgery, University of Pittsburgh, PA 15261, USA
    Transplantation 87:805-12. 2009
    ..The role of the innate immune system in the development of thrombotic microangiopathy (TM) after alpha1,3-galactosyltransferase gene-knockout (GTKO) pig organ transplantation in primates is uncertain...
  35. doi request reprint Islet xenotransplantation: the pig-to-non-human primate model
    Rita Bottino
    Division of Immunogenetics, Department of Pediatrics, Children s Hospital of Pittsburgh, Pittsburgh, PA 15213, USA
    Xenotransplantation 15:104-6. 2008
  36. ncbi request reprint Pig-to-non-human primate heart transplantation: immunologic progress over 20 years
    Xiaocheng Zhu
    Thomas E Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    J Heart Lung Transplant 26:210-8. 2007
    ..Genetic modifications to organ-source pigs have been important steps in increasing heart xenograft survival from a few minutes in 1986 to 2 to 6 months in 2005...
  37. ncbi request reprint The pig-to-primate immune response: relevance for xenotransplantation
    Hao Chih Tai
    Department of Surgery, the Thomas E Starzl Transplantation Institute, University of Pittsburgh Medical Center, Pittsburgh, PA 15261, USA
    Xenotransplantation 14:227-35. 2007
    ..host (GVH) response from the activity of donor B or T cells. We have investigated whether there is a risk of a GVH response following pig-to-primate organ xenotransplantation...
  38. ncbi request reprint Progress in xenotransplantation following the introduction of gene-knockout technology
    Hao Chih Tai
    Thomas E Starzl Transplantation Institute, University of Pittsburgh Medical Center, Pittsburgh, PA 15261, USA
    Transpl Int 20:107-17. 2007
    ..g. human tissue factor pathway inhibitor, might prevent the change in vascular endothelium from an anti-coagulant to a procoagulant phenotype, and protect the islets from early loss...
  39. ncbi request reprint Allosensitized humans are at no greater risk of humoral rejection of GT-KO pig organs than other humans
    Hidetaka Hara
    Thomas E Starzl Transplantation Institute, University of Pittsburgh Medical Center, Pittsburgh, PA 15261, USA
    Xenotransplantation 13:357-65. 2006
    ....
  40. ncbi request reprint Anti-Gal alpha 1-3Gal IgM and IgG antibody levels in sera of humans and old world non-human primates
    Katsuhito Teranishi
    Transplantation Biology Research Center, Massachusetts General Hospital Harvard Medical School, Boston, MA 02129, USA
    Xenotransplantation 9:148-54. 2002
    ..The measured differences in anti-Gal IgM and IgG levels may affect the kinetics of Ab removal and rate of return in different species, and thus may have relevance for translating work in non-human primate models to the clinical setting...
  41. ncbi request reprint Incidence and cytotoxicity of antibodies in cynomolgus monkeys directed to nonGal antigens, and their relevance for experimental models
    Pleunie P M Rood
    the Thomas E Starzl Transplantation Institute, University of Pittsburgh Medical Center, Pittsburgh, PA 15261, USA
    Transpl Int 19:158-65. 2006
    ....
  42. ncbi request reprint Modulation of the in vivo primate anti-Gal response through administration of anti-idiotypic antibodies
    Isabel M McMorrow
    Transplantation Biology Research Center, Massachusetts General Hospital Harvard Medical School, Boston 02129, USA
    Xenotransplantation 9:106-14. 2002
    ....
  43. ncbi request reprint alpha1,3-Galactosyltransferase gene-knockout pig heart transplantation in baboons with survival approaching 6 months
    Yau Lin Tseng
    Transplantation Biology Research Center, Massachusetts General Hospital Harvard Medical School, Boston, MA, USA
    Transplantation 80:1493-500. 2005
    ..The recent generation of alpha1,3-galactosyltransferase gene-knockout (GalT-KO) pigs has allowed investigation of the survival of GalT-KO pig organs in nonhuman primates...
  44. ncbi request reprint Marked prolongation of porcine renal xenograft survival in baboons through the use of alpha1,3-galactosyltransferase gene-knockout donors and the cotransplantation of vascularized thymic tissue
    Kazuhiko Yamada
    Transplantation Biology Research Center, Massachusetts General Hospital, Boston, Massachusetts 02129, USA
    Nat Med 11:32-4. 2005
    ..We have achieved life-supporting pig-to-baboon renal xenograft survivals of up to 83 d with normal creatinine levels...
  45. ncbi request reprint Thrombotic microangiopathy and graft arteriopathy in pig hearts following transplantation into baboons
    Stuart L Houser
    Department of Pathology, Massachusetts General Hospital Harvard Medical School, Boston, MA 02114, USA
    Xenotransplantation 11:416-25. 2004
    ..Acute humoral xenograft rejection (AHXR) is an immunologic barrier in pig-to-baboon organ transplantation (Tx). We report microvascular thrombosis and myocardial necrosis in a series of cardiac xenografts...
  46. ncbi request reprint Investigation of red blood cells from alpha1,3-galactosyltransferase-knockout pigs for human blood transfusion
    Foad J Rouhani
    Transplantation Biology Research Center, Massachusetts General Hospital Harvard Medical School, Boston, Massachusetts, USA
    Transfusion 44:1004-12. 2004
    ..alpha1,3-Galactosyltransferase gene-knockout pigs, which do not express Gal epitopes on RBCs (Gal-/-), have recently become available...
  47. doi request reprint Safe induction of diabetes by high-dose streptozotocin in pigs
    Hidetaka Hara
    Department of Surgery, Thomas E Starzl Transplantation Institute, University of Pittsburgh Medical Center, Pittsburgh, PA 15261, USA
    Pancreas 36:31-8. 2008
    ..As a result, pancreatectomy has been advocated in this species. We have investigated the effects of 2 dosages of STZ to safely induce diabetes in pigs...
  48. ncbi request reprint Pig kidney transplantation in baboons treated intravenously with a bovine serum albumin-Galalpha1-3Gal conjugate
    Bernd Gollackner
    Transplantation Biology Research Center, Massachusetts General Hospital Harvard Medical School, Boston, MA 02129, USA
    Xenotransplantation 10:606-14. 2003
    ..The mechanism of the hepatic injury remains uncertain...
  49. pmc Hepatic function after genetically engineered pig liver transplantation in baboons
    Burcin Ekser
    Thomas E Starzl Transplantation Institute, University of Pittsburgh Medical Center, Pittsburgh, PA, USA
    Transplantation 90:483-93. 2010
    ..If "bridging" to allo-transplantation (Tx) is to be achieved by a pig liver xenograft, adequate hepatic function needs to be assured...
  50. ncbi request reprint Correlation of biochemical and hematological changes with graft failure following pig heart and kidney transplantation in baboons
    Christoph Knosalla
    Transplantation Biology Research Center, Massachusetts General Hospital Harvard Medical School, Boston, MA, USA
    Am J Transplant 3:1510-9. 2003
    ..Noninvasive parameters were identified that have predictive potential for AHXR. Monitoring of these might enable therapeutic intervention to reverse rejection...
  51. ncbi request reprint Reduced efficacy of ganciclovir against porcine and baboon cytomegalovirus in pig-to-baboon xenotransplantation
    Nicolas J Mueller
    Infectious Diseases Division, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    Am J Transplant 3:1057-64. 2003
    ..GCV and other antiviral agents have limited activities against PCMV in vitro. Breeding of PCMV-free xenograft donors may be necessary to prevent PCMV infections in clinical trials...
  52. ncbi request reprint Porcine cytomegalovirus and coagulopathy in pig-to-primate xenotransplantation
    Bernd Gollackner
    Transplantation Biology Research Center, Massachusetts General Hospital Harvard Medical School, Boston, MA 02114, USA
    Transplantation 75:1841-7. 2003
    ..The activation of porcine (P) cytomegalovirus (PCMV) and baboon (B) CMV infections has been documented in pig-to-primate xenotransplantation. The purpose of this study was to determine the contribution of PCMV and BCMV to CC...
  53. ncbi request reprint Depletion of anti-Gal antibodies by the intravenous infusion of Gal type 2 and 6 glycoconjugates in baboons
    Katsuhito Teranishi
    Transplantation Biology Research Center, Massachusetts General Hospital Harvard Medical School, MGH East, Building 149 9019, Boston, MA 02129, USA
    Xenotransplantation 10:357-67. 2003
    ..v.) infusion of (i) bovine serum albumin conjugated to Gal type 6 oligosaccharides (BSA-Gal) or (ii) a poly l-lysine backbone conjugated to Gal type 2 or 6 oligosaccharides (PLL-Gal)...
  54. doi request reprint In vitro investigation of pig cells for resistance to human antibody-mediated rejection
    Hidetaka Hara
    Thomas E Starzl Transplantation Institute, Department of Surgery, University of Pittsburgh Medical Center, Pittsburgh, PA, USA
    Transpl Int 21:1163-74. 2008
    ..Organs from GTKO/CD46 pigs are likely to be significantly less susceptible to CDC...
  55. ncbi request reprint Xenogeneic thymus transplantation in a pig-to-baboon model
    Anette Wu
    Transplantation Biology Research Center, Massachusetts General Hospital Harvard Medical School, Boston, MA 02129, USA
    Transplantation 75:282-91. 2003
    ..We have tested whether fetal porcine thymic tissue transplantation can lead to tolerance across a discordant (pig-to-baboon) xenogeneic barrier...
  56. ncbi request reprint Suppression of natural and elicited antibodies in pig-to-baboon heart transplantation using a human anti-human CD154 mAb-based regimen
    Kenji Kuwaki
    Transplantation Biology Research Center, Massachusetts General Hospital Harvard Medical School, Boston, MA
    Am J Transplant 4:363-72. 2004
    ..The treatment regimen was effective in maintaining low anti-Gal Ab levels and in delaying or preventing AHXR. The combination of costimulatory blockade and heparin with Tx of a Gal-negative pig organ may prolong graft survival further...
  57. ncbi request reprint Initial investigation of the potential of modified porcine erythrocytes for transfusion in primates
    Jan M Eckermann
    Transplantation Biology Research Center, Massachusetts General Hospital Harvard Medical School, Boston, MA, USA
    Xenotransplantation 11:18-26. 2004
    ..To fully assess the potential of pRBC transfusion in humans, more complete alpha-galactosidase treatment of pRBCs will be required...
  58. doi request reprint The immunology of corneal xenotransplantation: a review of the literature
    Hidetaka Hara
    Thomas E Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, PA 15261, USA
    Xenotransplantation 17:338-49. 2010
    ..Recent progress in the genetic manipulation of pigs has led to the prospect that clinical corneal xenotransplantation, in the absence of exogenous immunosuppressive therapy, may become successful in the foreseeable future...
  59. ncbi request reprint Reducing Gal expression on the pig organ - a retrospective review
    Mohamed Ezzelarab
    Thomas E Starzl Transplantation Institute, University of Pittsburgh Medical Center, Pittsburgh, PA 15261, USA
    Xenotransplantation 12:278-85. 2005
    ....
  60. ncbi request reprint Isolation outcome and functional characteristics of young and adult pig pancreatic islets for transplantation studies
    Rita Bottino
    Division of Immunogenetics, Department of Pediatrics, Children s Hospital of Pittsburgh, Rangos Research Center, Pittsburgh, PA 15213, USA
    Xenotransplantation 14:74-82. 2007
    ..To limit the costs involved in the logistics and health care of pigs for clinical xenotransplantation, we have studied whether younger, rather than older, pigs that are typically preferred can be used as islet donors...
  61. pmc Pig liver xenotransplantation as a bridge to allotransplantation: which patients might benefit?
    Burcin Ekser
    Thomas E Starzl Transplantation Institute, University of Pittsburgh Medical Center, Pittsburgh, PA 15261, USA
    Transplantation 88:1041-9. 2009
    ....
  62. ncbi request reprint Which patients first? Planning the first clinical trial of xenotransplantation: a case for cardiac bridging
    Zuhaib Ibrahim
    Thomas E Starzl Transplantation Institute, University of Pittsburgh Medical Center, Pittsburgh, PA, USA
    Xenotransplantation 12:168-72. 2005
  63. doi request reprint An in vitro model of pig liver xenotransplantation--pig complement is associated with reduced lysis of wild-type and genetically modified pig cells
    Hidetaka Hara
    Thomas E Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, PA 15261, USA
    Xenotransplantation 17:370-8. 2010
    ..We investigated in vitro the lysis of wild-type (WT), α1,3-galactosyltransferase gene-knockout (GTKO), and CD46 transgenic (CD46) pig peripheral blood mononuclear cells (PBMC) caused by human anti-pig antibodies (Abs) + pig C...
  64. ncbi request reprint The potential of statins in xenotransplantation
    Mohamed Ezzelarab
    Thomas E Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, PA, USA corrected
    Xenotransplantation 14:100-3. 2007
  65. ncbi request reprint Babesia as a complication of immunosuppression following pig-to-baboon heart transplantation
    Mohamed Ezzelarab
    Thomas E Starzl Transplantation Institute, University of Pittsburgh Medical Center, Pittsburgh, PA 15261, USA corrected
    Xenotransplantation 14:162-5. 2007
    ..Immunosuppression can reduce the host's immune system, and result in proliferation of the parasite, leading to hemolysis and other features of infection, sometimes with fatal outcome...
  66. pmc Xenotransplantation--the future of corneal transplantation?
    Hidetaka Hara
    Department of Surgery, Thomas E Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, PA, USA
    Cornea 30:371-8. 2011
    ..There is every reason for optimism that corneal xeno-Tx will become a clinical reality within the next few years...
  67. ncbi request reprint Activation of porcine cytomegalovirus, but not porcine lymphotropic herpesvirus, in pig-to-baboon xenotransplantation
    Nicolas J Mueller
    Infectious Disease Division, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA
    J Infect Dis 189:1628-33. 2004
    ..Control immunosuppressed swine coinfected with PCMV and PLHV-1 had activation of PCMV but not PLHV-1. PCMV, but not PLHV-1, is activated in solid-organ xenotransplantation...
  68. doi request reprint Liver xenografts for the treatment of acute liver failure: clinical and experimental experience and remaining immunologic barriers
    Hidetaka Hara
    Thomas E Starzl Transplantation Institute, University of Pittsburgh Medical Center, Pittsburgh, PA 15261, USA
    Liver Transpl 14:425-34. 2008
    ....
  69. ncbi request reprint Reduction of consumptive coagulopathy using porcine cytomegalovirus-free cardiac porcine grafts in pig-to-primate xenotransplantation
    Nicolas J Mueller
    Infectious Diseases Division, Massachusetts General Hospital, Harvard Medical School, 55 Fruit Street, GRJ 504, Boston, MA 02114, USA
    Transplantation 78:1449-53. 2004
    ..Activation of porcine cytomegalovirus (PCMV) in pig-to-baboon xenotransplantation is associated with xenograft injury and possibly an increased incidence of consumptive coagulopathy (CC)...
  70. ncbi request reprint Carbohydrates in xenotransplantation
    Mohamed Ezzelarab
    Thomas E Starzl Transplantation Institute, University of Pittsburgh MedicalCenter, Pittsburgh, Pennsylvania 15261, USA
    Immunol Cell Biol 83:396-404. 2005
    ..There remain other antipig antibodies against hitherto unidentified antigenic targets that may well be involved in graft destruction; their possible carbohydrate target epitopes are discussed...
  71. ncbi request reprint How strong is the T cell response in the pig-to-primate model?
    Leo H Buhler
    University Hospital Geneva, Switzerland
    Xenotransplantation 12:85-7. 2005
  72. pmc Histopathology of spleen allograft rejection in miniature swine
    Frank J M F Dor
    Transplantation Biology Research Center, Massachusetts General Hospital Harvard Medical School, Boston, MA 02114, USA
    Int J Exp Pathol 86:57-66. 2005
    ..This study establishes histologic guidelines for diagnosing and, perhaps, in future studies, predicting acute rejection of splenic allografts transplanted across known histocompatibility barriers in a large-animal model...
  73. ncbi request reprint Will the pig solve the transplantation backlog?
    David K C Cooper
    Transplantation Biology Research Center, Massachusetts General Hospital Harvard Medical School, Boston, Massachusetts 02129, USA
    Annu Rev Med 53:133-47. 2002
    ..Finally, the question of patient and societal acceptance of xenotransplantation is discussed...
  74. pmc Expression of tissue factor and initiation of clotting by human platelets and monocytes after incubation with porcine endothelial cells
    Chih Che Lin
    Department of Immunology, Imperial College London, Hammersmith Hospital, London, United Kingdom
    Transplantation 86:702-9. 2008
    ..However, the precise factors initiating thrombosis are unknown. In this study, we investigated the contribution of recipient platelets and monocytes...
  75. ncbi request reprint Non-specific removal of antibodies in patients with idiopathic dilated cardiomyopathy: implications for xenotransplantation
    Christoph Knosalla
    Department of Cardiothoracic and Vascular Surgery, Deutsches Herzzentrum Berlin, Berlin, Germany
    J Heart Lung Transplant 23:623-6. 2004
    ..Extracorporeal immunoadsorption proved safe in patients with heart failure and was effective in depleting anti-Gal antibody and in decreasing serum cytotoxicity to pig cells...
  76. ncbi request reprint Xenotransplantation and tolerance
    Christoph Knosalla
    Transplantation Biology Research Center, Massachusetts General Hospital Harvard Medical School, Boston 02129, USA
    Front Biosci 7:d1280-7. 2002
    ..This review discusses the barriers to xenogeneic organ transplantation and the approaches that are being developed to overcome them, with the emphasis on methods that attempt to induce tolerance...
  77. ncbi request reprint Activation of human endothelial cells by mobilized porcine leukocytes in vitro: implications for mixed chimerism in xenotransplantation
    James Z Appel
    Transplantation Biology Research Center, Massachusetts General Hospital Harvard Medical School, Boston, MA 02215, USA
    Transplantation 73:1302-9. 2002
    ..Because the mechanisms responsible for TM are unclear, we have explored the effects of PBPC on human umbilical vein endothelial cell (HUVEC) activation...
  78. pmc Activation of cytomegalovirus in pig-to-primate organ xenotransplantation
    Nicolas J Mueller
    Infectious Diseases Division, Massachusetts General Hospital and Harvard Medical School, Boston 02114, USA
    J Virol 76:4734-40. 2002
    ..The observation of graft injury by PCMV demonstrates that CMV will be an important pathogen in immunosuppressed xenograft recipients. Strategies must be developed to exclude CMV from porcine organ donors...
  79. pmc Acute rejection is associated with antibodies to non-Gal antigens in baboons using Gal-knockout pig kidneys
    Gang Chen
    1 Department of Surgery, University of Western Ontario, London, Ontario N6A 5C1, Canada
    Nat Med 11:1295-8. 2005
    ..These data suggest that antibodies to non-Gal antigens will present an additional barrier to transplantation of organs from GalT-KO pigs to humans...
  80. ncbi request reprint Immunological unresponsiveness in chimeric miniature swine following MHC-mismatched spleen transplantation
    Frank J M F Dor
    Transplantation Biology Research Center, Massachusetts General Hospital Harvard Medical School, Boston, MA, USA
    Transplantation 80:1791-804. 2005
    ..In rodents, spleen allotransplantation (SpTx) induces tolerance. We investigated the induction of chimerism and donor-specific unresponsiveness following pig SpTx...
  81. ncbi request reprint Xenotransplantation--will tolerance be essential?
    David K C Cooper
    Nephrol Dial Transplant 21:2991-2; author reply 2992-3. 2006
  82. ncbi request reprint Can spleen transplantation induce tolerance? A review of the literature
    Frank J M F Dor
    Transplantation Biology Research Center, Massachusetts General Hospital, Havard Medical School, MGH East, Building 149 9019 13th Street, Boston, Massachusetts, MA 02129, USA
    Transpl Int 16:451-60. 2003
    ..Such a study is ongoing at our center...
  83. ncbi request reprint Allosensitization does not increase the risk of xenoreactivity to alpha1,3-galactosyltransferase gene-knockout miniature swine in patients on transplantation waiting lists
    Banny S Wong
    Transplantation Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Boston, 02129, USA
    Transplantation 82:314-9. 2006
    ..We have investigated this question using sera from such patients on GalT-KO target cells...
  84. ncbi request reprint ABO-incompatible allotransplantation as a basis for clinical xenotransplantation
    Georg Stussi
    Laboratory for Transplantation Immunology, Department of Internal Medicine, University Hospital Zurich, Zurich, Switzerland
    Xenotransplantation 13:390-9. 2006
    ..The similarities and differences between ABO-incompatible allotransplantation and xenotransplantation are discussed...
  85. ncbi request reprint Rene Kuss's clinical experience with pig kidney transplantation in 1966
    David K C Cooper
    Xenotransplantation 14:93. 2007
  86. ncbi request reprint Selected physiologic compatibilities and incompatibilities between human and porcine organ systems
    Zuhaib Ibrahim
    Thomas E Starzl Transplantation Institute, University of Pittsburgh Medical Center, Pittsburgh, PA 15261, USA
    Xenotransplantation 13:488-99. 2006
    ..Some of these physiologic differences can be overcome by producing genetically altered pigs to improve compatibility with humans. The possibility of producing such pigs for organ transplantation is considered...
  87. ncbi request reprint Response to commentaries on "alpha1,3-galactosyltransferase gene-knockout pigs for xenotransplantation: where do we go from here?"
    David K C Cooper
    Transplantation 84:1212-3. 2007
  88. doi request reprint High-throughput screening of glycan-binding proteins using miniature pig kidney N-glycan-immobilized beads
    Yun Gon Kim
    Interdisciplinary Program for Biochemical Engineering and Biotechnology, Seoul National University, Seoul 151 742, Korea
    Chem Biol 15:215-23. 2008
    ..Thus, these results demonstrate that the GBP screening method was firmly established by one-step immobilization of the N-glycans on to microsphere and highly sensitive mass-spectrometric analysis...
  89. ncbi request reprint Highlights of the 6th Congress of the International Xenotransplantation Association, Chicago, 2001
    Michael E Breimer
    Xenotransplantation 9:81-90. 2002

Research Grants4

  1. Thromboregulation to Prevent Thrombotic Microangiopathy
    David Cooper; Fiscal Year: 2007
    ..It is anticipated that these approaches will abrogate the development of TM and allow long-term survival of pig organs in baboons. ..
  2. Tolerance to Carbohydrate Antigens in Transplantation
    David Cooper; Fiscal Year: 2007
    ....