Nicholas K Conrad

Summary

Affiliation: University of Texas Southwestern Medical Center
Country: USA

Publications

  1. doi request reprint New insights into the expression and functions of the Kaposi's sarcoma-associated herpesvirus long noncoding PAN RNA
    Nicholas K Conrad
    Department of Microbiology, UT Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, TX 75390, United States Electronic address
    Virus Res 212:53-63. 2016
  2. doi request reprint Posttranscriptional gene regulation in Kaposi's sarcoma-associated herpesvirus
    Nicholas K Conrad
    Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9048, USA
    Adv Appl Microbiol 68:241-61. 2009
  3. pmc Chromatin immunoprecipitation and microarray analysis suggest functional cooperation between Kaposi's Sarcoma-associated herpesvirus ORF57 and K-bZIP
    Olga V Hunter
    UT Southwestern Medical Center, Department of Microbiology, Dallas, Texas, USA
    J Virol 87:4005-16. 2013
  4. doi request reprint Canonical Poly(A) Polymerase Activity Promotes the Decay of a Wide Variety of Mammalian Nuclear RNAs
    Stefan M Bresson
    Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America
    PLoS Genet 11:e1005610. 2015
  5. pmc Delineation of a core RNA element required for Kaposi's sarcoma-associated herpesvirus ORF57 binding and activity
    Emi Sei
    Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9048, USA
    Virology 419:107-16. 2011
  6. pmc HITS-CLIP analysis uncovers a link between the Kaposi's sarcoma-associated herpesvirus ORF57 protein and host pre-mRNA metabolism
    Emi Sei
    Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America
    PLoS Pathog 11:e1004652. 2015
  7. pmc Kaposi's sarcoma-associated herpesvirus ORF57 protein binds and protects a nuclear noncoding RNA from cellular RNA decay pathways
    Brooke B Sahin
    Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America
    PLoS Pathog 6:e1000799. 2010
  8. pmc Viral factors reveal a role for REF/Aly in nuclear RNA stability
    Sarah H Stubbs
    Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas, USA
    Mol Cell Biol 32:1260-70. 2012
  9. pmc The emerging role of triple helices in RNA biology
    Nicholas K Conrad
    Department of Microbiology, UT Southwestern Medical Center, Dallas, TX, USA
    Wiley Interdiscip Rev RNA 5:15-29. 2014
  10. pmc Depletion of REF/Aly alters gene expression and reduces RNA polymerase II occupancy
    Sarah H Stubbs
    Department of Microbiology, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, TX 75390 9048, USA
    Nucleic Acids Res 43:504-19. 2015

Collaborators

  • Yang Xie
  • Tao Wang
  • Emi Sei
  • Sarah H Stubbs
  • Olga V Hunter
  • Stefan M Bresson
  • Brooke B Sahin
  • Allyson C Hunter
  • R Blake Richardson
  • Ashley Hoover
  • Denish Patel

Detail Information

Publications13

  1. doi request reprint New insights into the expression and functions of the Kaposi's sarcoma-associated herpesvirus long noncoding PAN RNA
    Nicholas K Conrad
    Department of Microbiology, UT Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, TX 75390, United States Electronic address
    Virus Res 212:53-63. 2016
    ..In this review, I highlight the mechanisms of PAN RNA accumulation and describe recent insights into potential functions of PAN RNA. ..
  2. doi request reprint Posttranscriptional gene regulation in Kaposi's sarcoma-associated herpesvirus
    Nicholas K Conrad
    Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9048, USA
    Adv Appl Microbiol 68:241-61. 2009
    ..With the growing appreciation of posttranscriptional regulation in cellular gene expression, it seems likely that the list of viral posttranscriptional regulatory schemes will expand as new details of KSHV gene regulation are uncovered...
  3. pmc Chromatin immunoprecipitation and microarray analysis suggest functional cooperation between Kaposi's Sarcoma-associated herpesvirus ORF57 and K-bZIP
    Olga V Hunter
    UT Southwestern Medical Center, Department of Microbiology, Dallas, Texas, USA
    J Virol 87:4005-16. 2013
    ..Our data demonstrate that ORF57 associates with the viral genome by using at least two modes of recruitment, and they suggest that ORF57 and K-bZIP coregulate viral gene expression during lytic infection...
  4. doi request reprint Canonical Poly(A) Polymerase Activity Promotes the Decay of a Wide Variety of Mammalian Nuclear RNAs
    Stefan M Bresson
    Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America
    PLoS Genet 11:e1005610. 2015
    ..We conclude that PPD is an important mammalian nuclear RNA decay pathway for the removal of poorly spliced and nuclear-retained transcripts. ..
  5. pmc Delineation of a core RNA element required for Kaposi's sarcoma-associated herpesvirus ORF57 binding and activity
    Emi Sei
    Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9048, USA
    Virology 419:107-16. 2011
    ..These studies provide insight into ORF57 specificity determinants and lay a foundation for future analyses of cellular and viral ORF57 targets...
  6. pmc HITS-CLIP analysis uncovers a link between the Kaposi's sarcoma-associated herpesvirus ORF57 protein and host pre-mRNA metabolism
    Emi Sei
    Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America
    PLoS Pathog 11:e1004652. 2015
    ..These data suggest that ORF57 is involved in modulating host gene expression in addition to KSHV gene expression during lytic reactivation...
  7. pmc Kaposi's sarcoma-associated herpesvirus ORF57 protein binds and protects a nuclear noncoding RNA from cellular RNA decay pathways
    Brooke B Sahin
    Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America
    PLoS Pathog 6:e1000799. 2010
    ..We propose that KSHV ORF57 protein functions to enhance the nuclear stability of intronless viral transcripts by protecting them from a cellular RNA quality control pathway...
  8. pmc Viral factors reveal a role for REF/Aly in nuclear RNA stability
    Sarah H Stubbs
    Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas, USA
    Mol Cell Biol 32:1260-70. 2012
    ..These studies show that REF/Aly can stabilize nuclear RNAs independently of their export and support a broader conservation of RNA quality control mechanisms from yeast to humans...
  9. pmc The emerging role of triple helices in RNA biology
    Nicholas K Conrad
    Department of Microbiology, UT Southwestern Medical Center, Dallas, TX, USA
    Wiley Interdiscip Rev RNA 5:15-29. 2014
    ..These examples highlight the diverse roles that RNA triple helices play in biology. Moreover, the dissection of triple helix mechanisms has the potential to uncover fundamental pathways in cell biology...
  10. pmc Depletion of REF/Aly alters gene expression and reduces RNA polymerase II occupancy
    Sarah H Stubbs
    Department of Microbiology, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, TX 75390 9048, USA
    Nucleic Acids Res 43:504-19. 2015
    ..Our data are consistent with the model that REF/Aly is involved in linking splicing with transcription efficiency. ..
  11. doi request reprint UV cross-linking of interacting RNA and protein in cultured cells
    Emi Sei
    Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX, USA
    Methods Enzymol 539:53-66. 2014
    ..This protocol couples ultraviolet (UV) irradiation with IP to determine whether a specific protein interacts directly with a specific RNA in living cells. ..
  12. doi request reprint Analysis of RNA-protein interactions by cell mixing
    Sarah H Stubbs
    Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX, USA
    Methods Enzymol 539:67-80. 2014
    ..Before concluding that a specific RNA and protein interact in vivo, cell-mixing experiments can be performed to ensure that observed RNA-protein complexes are not formed after lysis of cells. ..
  13. pmc The human nuclear poly(a)-binding protein promotes RNA hyperadenylation and decay
    Stefan M Bresson
    Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America
    PLoS Genet 9:e1003893. 2013
    ..Our results highlight a complex relationship between PABPN1, PAPα/γ, and nuclear RNA decay, and we suggest that these activities may play broader roles in the regulation of human gene expression. ..