Nicholas K Conrad

Summary

Affiliation: University of Texas Southwestern Medical Center
Country: USA

Publications

  1. pmc Kaposi's sarcoma-associated herpesvirus ORF57 protein binds and protects a nuclear noncoding RNA from cellular RNA decay pathways
    Brooke B Sahin
    Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America
    PLoS Pathog 6:e1000799. 2010
  2. doi request reprint Posttranscriptional gene regulation in Kaposi's sarcoma-associated herpesvirus
    Nicholas K Conrad
    Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9048, USA
    Adv Appl Microbiol 68:241-61. 2009
  3. pmc Chromatin immunoprecipitation and microarray analysis suggest functional cooperation between Kaposi's Sarcoma-associated herpesvirus ORF57 and K-bZIP
    Olga V Hunter
    UT Southwestern Medical Center, Department of Microbiology, Dallas, Texas, USA
    J Virol 87:4005-16. 2013
  4. pmc Viral factors reveal a role for REF/Aly in nuclear RNA stability
    Sarah H Stubbs
    Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas, USA
    Mol Cell Biol 32:1260-70. 2012
  5. pmc Delineation of a core RNA element required for Kaposi's sarcoma-associated herpesvirus ORF57 binding and activity
    Emi Sei
    Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9048, USA
    Virology 419:107-16. 2011
  6. ncbi request reprint UV cross-linking of interacting RNA and protein in cultured cells
    Emi Sei
    Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX, USA
    Methods Enzymol 539:53-66. 2014
  7. pmc The human nuclear poly(a)-binding protein promotes RNA hyperadenylation and decay
    Stefan M Bresson
    Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America
    PLoS Genet 9:e1003893. 2013
  8. ncbi request reprint Analysis of RNA-protein interactions by cell mixing
    Sarah H Stubbs
    Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX, USA
    Methods Enzymol 539:67-80. 2014

Research Grants

Collaborators

  • Emi Sei
  • Sarah H Stubbs
  • Olga V Hunter
  • Stefan M Bresson
  • Brooke B Sahin
  • R Blake Richardson
  • Ashley Hoover
  • Denish Patel

Detail Information

Publications8

  1. pmc Kaposi's sarcoma-associated herpesvirus ORF57 protein binds and protects a nuclear noncoding RNA from cellular RNA decay pathways
    Brooke B Sahin
    Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America
    PLoS Pathog 6:e1000799. 2010
    ..We propose that KSHV ORF57 protein functions to enhance the nuclear stability of intronless viral transcripts by protecting them from a cellular RNA quality control pathway...
  2. doi request reprint Posttranscriptional gene regulation in Kaposi's sarcoma-associated herpesvirus
    Nicholas K Conrad
    Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9048, USA
    Adv Appl Microbiol 68:241-61. 2009
    ..With the growing appreciation of posttranscriptional regulation in cellular gene expression, it seems likely that the list of viral posttranscriptional regulatory schemes will expand as new details of KSHV gene regulation are uncovered...
  3. pmc Chromatin immunoprecipitation and microarray analysis suggest functional cooperation between Kaposi's Sarcoma-associated herpesvirus ORF57 and K-bZIP
    Olga V Hunter
    UT Southwestern Medical Center, Department of Microbiology, Dallas, Texas, USA
    J Virol 87:4005-16. 2013
    ..Our data demonstrate that ORF57 associates with the viral genome by using at least two modes of recruitment, and they suggest that ORF57 and K-bZIP coregulate viral gene expression during lytic infection...
  4. pmc Viral factors reveal a role for REF/Aly in nuclear RNA stability
    Sarah H Stubbs
    Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas, USA
    Mol Cell Biol 32:1260-70. 2012
    ..These studies show that REF/Aly can stabilize nuclear RNAs independently of their export and support a broader conservation of RNA quality control mechanisms from yeast to humans...
  5. pmc Delineation of a core RNA element required for Kaposi's sarcoma-associated herpesvirus ORF57 binding and activity
    Emi Sei
    Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9048, USA
    Virology 419:107-16. 2011
    ..These studies provide insight into ORF57 specificity determinants and lay a foundation for future analyses of cellular and viral ORF57 targets...
  6. ncbi request reprint UV cross-linking of interacting RNA and protein in cultured cells
    Emi Sei
    Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX, USA
    Methods Enzymol 539:53-66. 2014
    ..This protocol couples ultraviolet (UV) irradiation with IP to determine whether a specific protein interacts directly with a specific RNA in living cells. ..
  7. pmc The human nuclear poly(a)-binding protein promotes RNA hyperadenylation and decay
    Stefan M Bresson
    Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America
    PLoS Genet 9:e1003893. 2013
    ..Our results highlight a complex relationship between PABPN1, PAPα/γ, and nuclear RNA decay, and we suggest that these activities may play broader roles in the regulation of human gene expression. ..
  8. ncbi request reprint Analysis of RNA-protein interactions by cell mixing
    Sarah H Stubbs
    Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX, USA
    Methods Enzymol 539:67-80. 2014
    ..Before concluding that a specific RNA and protein interact in vivo, cell-mixing experiments can be performed to ensure that observed RNA-protein complexes are not formed after lysis of cells. ..

Research Grants1

  1. Mechanisms of KSHV post-transcriptional gene regulation
    Nicholas K Conrad; Fiscal Year: 2010
    ..A deeper understanding of KSHV gene regulation will lead to a more complete knowledge of this important human pathogen and may reveal aspects of gene regulation in its human host cell. ..