T D Connell

Summary

Affiliation: University at Buffalo
Country: USA

Publications

  1. pmc Enhanced antigen uptake by dendritic cells induced by the B pentamer of the type II heat-labile enterotoxin LT-IIa requires engagement of TLR2
    Chang Hoon Lee
    The Department of Microbiology and Immunology, The University at Buffalo, NY 14214, USA
    Vaccine 28:3696-705. 2010
  2. pmc Iron starvation of Bordetella avium stimulates expression of five outer membrane proteins and regulates a gene involved in acquiring iron from serum
    T D Connell
    Center for Microbial Pathogenesis and the Department of Microbiology, School of Medicine and Biomedical Sciences, State University of New York at Buffalo, Buffalo, New York 14214, USA
    Infect Immun 66:3597-605. 1998
  3. pmc Cholera toxin, LT-I, LT-IIa and LT-IIb: the critical role of ganglioside binding in immunomodulation by type I and type II heat-labile enterotoxins
    Terry D Connell
    School of Medicine and Biomedical Sciences, Department of Microbiology and Immunology, 138 Farber Hall, 3435 Main St, Buffalo, NY 14214, USA
    Expert Rev Vaccines 6:821-34. 2007
  4. pmc Binding to gangliosides containing N-acetylneuraminic acid is sufficient to mediate the immunomodulatory properties of the nontoxic mucosal adjuvant LT-IIb(T13I)
    Hesham F Nawar
    Witebsky Center for Microbial Pathogenesis and Immunology and Department of Microbiology and Immunology, University at Buffalo, State University of New York at Buffalo, Buffalo, New York 14214, USA
    Clin Vaccine Immunol 17:969-78. 2010
  5. pmc Mutants of type II heat-labile enterotoxin LT-IIa with altered ganglioside-binding activities and diminished toxicity are potent mucosal adjuvants
    Hesham F Nawar
    The Witebsky Center for Microbial Pathogenesis and Immunology, The Department of Microbiology and Immunology, University at Buffalo, Buffalo, New York 14214, USA
    Infect Immun 75:621-33. 2007
  6. pmc Mucosal adjuvant properties of mutant LT-IIa and LT-IIb enterotoxins that exhibit altered ganglioside-binding activities
    Hesham F Nawar
    Department of Microbiology and Immunology, School of Medicine and Biomedical Sciences, State University of New York at Buffalo, 138 Farber Hall, 3435 Main St, Buffalo, NY 14214, USA
    Infect Immun 73:1330-42. 2005
  7. ncbi request reprint Immunostimulatory activity of LT-IIa, a type II heat-labile enterotoxin of Escherichia coli
    T D Connell
    Department of Microbiology, School of Medicine and Biomedical Sciences, The State University of New York at Buffalo, 14214, USA
    Immunol Lett 62:117-20. 1998
  8. pmc Endochitinase is transported to the extracellular milieu by the eps-encoded general secretory pathway of Vibrio cholerae
    T D Connell
    Center for Microbial Pathogenesis and Department of Microbiology, School of Medicine and Biomedical Sciences, State University of New York at Buffalo, Buffalo, New York 14214, USA
    J Bacteriol 180:5591-600. 1998
  9. ncbi request reprint Pituitary adenylate cyclase-activating polypeptide and vasoactive intestinal peptide inhibit dendritic growth in cultured sympathetic neurons
    Karen Drahushuk
    Department of Pharmacology and Toxicology and the Witebsky Center for Microbial Pathogenesis and Immunology, State University of New York at Buffalo, Buffalo, New York 14214, USA
    J Neurosci 22:6560-9. 2002
  10. pmc Mammalian cell ganglioside-binding specificities of E. coli enterotoxins LT-IIb and variant LT-IIb(T13I)
    Charles S Berenson
    Infectious Disease Division, Department of Veterans Affairs Western New York Healthcare System, State University of New York at Buffalo School of Medicine, Buffalo, NY 14215, USA
    Glycobiology 20:41-54. 2010

Research Grants

  1. Mechanisms of Adjuvant Stimulation
    TERRY CONNELL; Fiscal Year: 2006

Collaborators

Detail Information

Publications27

  1. pmc Enhanced antigen uptake by dendritic cells induced by the B pentamer of the type II heat-labile enterotoxin LT-IIa requires engagement of TLR2
    Chang Hoon Lee
    The Department of Microbiology and Immunology, The University at Buffalo, NY 14214, USA
    Vaccine 28:3696-705. 2010
    ..10 CD4(+) T cell proliferation assay. These experiments confirm that LT-IIa-B(5) exhibits potent immunomodulatory properties which may be exploitable as a non-toxic mucosal adjuvant...
  2. pmc Iron starvation of Bordetella avium stimulates expression of five outer membrane proteins and regulates a gene involved in acquiring iron from serum
    T D Connell
    Center for Microbial Pathogenesis and the Department of Microbiology, School of Medicine and Biomedical Sciences, State University of New York at Buffalo, Buffalo, New York 14214, USA
    Infect Immun 66:3597-605. 1998
    ..The data indicated that the mutation in Pho-20 affected expression of one or more components of an uptake machinery that is involved in acquisition of iron from organic ferricomplexes...
  3. pmc Cholera toxin, LT-I, LT-IIa and LT-IIb: the critical role of ganglioside binding in immunomodulation by type I and type II heat-labile enterotoxins
    Terry D Connell
    School of Medicine and Biomedical Sciences, Department of Microbiology and Immunology, 138 Farber Hall, 3435 Main St, Buffalo, NY 14214, USA
    Expert Rev Vaccines 6:821-34. 2007
    ..Mutant enterotoxins with ablated or altered ganglioside-binding affinities have been employed to investigate the role of gangliosides in enterotoxin-dependent immunomodulation...
  4. pmc Binding to gangliosides containing N-acetylneuraminic acid is sufficient to mediate the immunomodulatory properties of the nontoxic mucosal adjuvant LT-IIb(T13I)
    Hesham F Nawar
    Witebsky Center for Microbial Pathogenesis and Immunology and Department of Microbiology and Immunology, University at Buffalo, State University of New York at Buffalo, Buffalo, New York 14214, USA
    Clin Vaccine Immunol 17:969-78. 2010
    ..The nontoxic mutant enterotoxin LT-IIb(T13I), therefore, is potentially a new and safe human mucosal adjuvant...
  5. pmc Mutants of type II heat-labile enterotoxin LT-IIa with altered ganglioside-binding activities and diminished toxicity are potent mucosal adjuvants
    Hesham F Nawar
    The Witebsky Center for Microbial Pathogenesis and Immunology, The Department of Microbiology and Immunology, University at Buffalo, Buffalo, New York 14214, USA
    Infect Immun 75:621-33. 2007
    ..LT-IIa(T14I) and LT-IIa(T14D) had extremely diminished toxicities in a mouse Y1 adrenal cell bioassay and reduced abilities to induce the accumulation of intracellular cyclic AMP in a macrophage cell line...
  6. pmc Mucosal adjuvant properties of mutant LT-IIa and LT-IIb enterotoxins that exhibit altered ganglioside-binding activities
    Hesham F Nawar
    Department of Microbiology and Immunology, School of Medicine and Biomedical Sciences, State University of New York at Buffalo, 138 Farber Hall, 3435 Main St, Buffalo, NY 14214, USA
    Infect Immun 73:1330-42. 2005
    ..In contrast, LT-IIb(T13I) retained binding activity for T cells, B cells, and macrophages, indicating that this immunostimulatory mutant enterotoxin interacts with one or more unknown lymphoid cell receptors...
  7. ncbi request reprint Immunostimulatory activity of LT-IIa, a type II heat-labile enterotoxin of Escherichia coli
    T D Connell
    Department of Microbiology, School of Medicine and Biomedical Sciences, The State University of New York at Buffalo, 14214, USA
    Immunol Lett 62:117-20. 1998
    ..Recombinant methods have been established for producing large amounts of LT-IIa, an advantage that will likely provide an economic impetus to consider incorporating the enterotoxin as an immunostimulatory agent in future vaccines...
  8. pmc Endochitinase is transported to the extracellular milieu by the eps-encoded general secretory pathway of Vibrio cholerae
    T D Connell
    Center for Microbial Pathogenesis and Department of Microbiology, School of Medicine and Biomedical Sciences, State University of New York at Buffalo, Buffalo, New York 14214, USA
    J Bacteriol 180:5591-600. 1998
    ..These results indicate that ChiA and cholera toxin have functionally similar extracellular transport signals that are essential for eps-dependent secretion...
  9. ncbi request reprint Pituitary adenylate cyclase-activating polypeptide and vasoactive intestinal peptide inhibit dendritic growth in cultured sympathetic neurons
    Karen Drahushuk
    Department of Pharmacology and Toxicology and the Witebsky Center for Microbial Pathogenesis and Immunology, State University of New York at Buffalo, Buffalo, New York 14214, USA
    J Neurosci 22:6560-9. 2002
    ..These data suggest that peptides released by preganglionic nerves modulate dendritic growth in sympathetic neurons by a cAMP-dependent mechanism...
  10. pmc Mammalian cell ganglioside-binding specificities of E. coli enterotoxins LT-IIb and variant LT-IIb(T13I)
    Charles S Berenson
    Infectious Disease Division, Department of Veterans Affairs Western New York Healthcare System, State University of New York at Buffalo School of Medicine, Buffalo, NY 14215, USA
    Glycobiology 20:41-54. 2010
    ..These studies underscore the high degree of specificity required for ganglioside-protein interactions...
  11. pmc Differential binding of Escherichia coli enterotoxins LT-IIa and LT-IIb and of cholera toxin elicits differences in apoptosis, proliferation, and activation of lymphoid cells
    Sergio Arce
    Department of Microbiology and Immunology, The University of Buffalo, State University of New York at Buffalo, 138 Farber Hall, 3435 Main St, Buffalo, NY 12214, USA
    Infect Immun 73:2718-27. 2005
    ..Thus, LT-IIa, LT-IIb, and CT exhibit distinguishable immunomodulatory properties which are likely dependent upon their capacities to recognize different ganglioside receptors on lymphocytes...
  12. pmc In vitro induction of immunoglobulin A (IgA)- and IgM-secreting plasma blasts by cholera toxin depends on T-cell help and is mediated by CD154 up-regulation and inhibition of gamma interferon synthesis
    Sergio Arce
    The Department of Microbiology and Immunology, School of Medicine and Biomedical Sciences, University at Buffalo, SUNY, 138 Farber Hall, 3435 Main St, Buffalo, NY 14214, USA
    Infect Immun 75:1413-23. 2007
    ..Neither LT-IIa or LT-IIb had these effects, indicating that type II enterotoxins augment Ab responses by other mechanisms...
  13. pmc Novel iron-regulated and Fur-regulated small regulatory RNAs in Aggregatibacter actinomycetemcomitans
    J J Amarasinghe
    Department of Oral Biology, School of Dental Medicine, University at Buffalo, State University of New York, Buffalo, NY 14214, USA
    Mol Oral Microbiol 27:327-49. 2012
    ..In summary, these sRNAs have the ability to modulate biofilm formation, but their functional target genes remain to be confirmed...
  14. pmc Heme utilization in Bordetella avium is regulated by RhuI, a heme-responsive extracytoplasmic function sigma factor
    A E Kirby
    Witebsky Center for Microbial Pathogenesis and Immunology and Department of Microbiology, School of Medicine and Biomedical Sciences, The State University of New York, Buffalo, New York 14214, USA
    Infect Immun 69:6951-61. 2001
    ..Heme, hemoglobin, and several other heme-containing proteins were shown to be the extracellular inducers of the rhuI-dependent regulatory system. Fur titration assays indicated that expression of rhuI was probably Fur dependent...
  15. pmc Expression of hurP, a gene encoding a prospective site 2 protease, is essential for heme-dependent induction of bhuR in Bordetella bronchiseptica
    Natalie D King-Lyons
    The Witebsky Center for Microbial Pathogenesis and Immunology and the Department of Microbiology and Immunology, The University at Buffalo, 3435 Main St, Buffalo, NY 14221, USA
    J Bacteriol 189:6266-75. 2007
    ..The data are consistent with a model in which HurP functionally modifies HurR, a sigma factor regulator that is essential for heme-dependent induction of bhuR...
  16. pmc LT-IIc, a new member of the type II heat-labile enterotoxin family encoded by an Escherichia coli strain obtained from a nonmammalian host
    Hesham F Nawar
    Witebsky Center for Microbial Pathogenesis and Immunology, University at Buffalo, Buffalo, NY 14214, USA
    Infect Immun 78:4705-13. 2010
    ..The data suggest that other types of the type II HLT subfamily are circulating in the environment and that host specificity of type II HLT is likely governed by changes in the B polypeptide which mediate binding to receptors...
  17. pmc Ganglioside-linked terminal sialic acid moieties on murine macrophages function as attachment receptors for murine noroviruses
    Stefan Taube
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan 48109 5620, USA
    J Virol 83:4092-101. 2009
    ..In conclusion, our data indicate that MNV can use terminal SA on gangliosides as attachment receptors during binding to murine macrophages...
  18. pmc Expression of BfrH, a putative siderophore receptor of Bordetella bronchiseptica, is regulated by iron, Fur1, and the extracellular function sigma factor EcfI
    Jonathan M Burgos
    The Department of Microbiology and Immunology, School of Medicine and Biomedical Sciences, The University at Buffalo, Buffalo, NY 14214, USA
    Infect Immun 78:1147-62. 2010
    ..bronchiseptica. These experiments revealed ecfIR-bfrH as a locus encoding a new member of the growing family of Fe and ECF sigma factor-modulated regulons in the bordetellae...
  19. pmc Passively released heme from hemoglobin and myoglobin is a potential source of nutrient iron for Bordetella bronchiseptica
    Jeffrey C Mocny
    Department of Microbiology and Immunology, The University at Buffalo, NY 14221, USA
    Infect Immun 75:4857-66. 2007
    ..These results also suggest that spontaneous hemin loss by hemoglobin and myoglobin may be a common mechanism by which many pathogenic bacteria acquire heme and heme-bound Fe...
  20. pmc BhuR, a virulence-associated outer membrane protein of Bordetella avium, is required for the acquisition of iron from heme and hemoproteins
    Erin R Murphy
    The Witebsky Center for Microbial Pathogenesis and Immunology, School of Medicine and Biomedical Sciences, University at Buffalo, State University of New York, Buffalo, New York 14214, USA
    Infect Immun 70:5390-403. 2002
    ..avium to demonstrate that the bacterium transports heme into the cytoplasm in a BhuR-dependent manner. The role of BhuR in virulence was established in turkey poults by use of a competitive-infection model...
  21. pmc Transcriptional control of the rhuIR-bhuRSTUV heme acquisition locus in Bordetella avium
    Natalie D King
    Witebsky Center for Microbial Pathogenesis and Immunology, University at Buffalo, State University of New York, 138 Farber Hall, 3435 Main St, Buffalo, NY 14214, USA
    Infect Immun 73:1613-24. 2005
    ..Fe also repressed transcription from PbhuR in a fur-dependent manner; however, activation from this promoter, in the presence or absence of heme, did not occur without rhuI...
  22. pmc The extracellular transport signal of the Vibrio cholerae endochitinase (ChiA) is a structural motif located between amino acids 75 and 555
    Jason P Folster
    The Witebsky Center for Microbial Pathogenesis and Immunology and Department of Microbiology, School of Medicine and Biomedical Sciences, The University of Buffalo, State University of New York, Buffalo, New York 14214, USA
    J Bacteriol 184:2225-34. 2002
    ....
  23. ncbi request reprint Ganglioside GD1a is an essential coreceptor for Toll-like receptor 2 signaling in response to the B subunit of type IIb enterotoxin
    Shuang Liang
    Center for Oral Health and Systemic Disease and Department of Periodontics, University of Louisville Health Sciences Center, Louisville, Kentucky 40292, USA
    J Biol Chem 282:7532-42. 2007
    ..These findings establish a model of TLR2 coreceptor function and, moreover, suggest novel mechanisms of adjuvanticity by non-toxic derivatives of type II enterotoxins dependent upon GD1a/TLR2 cooperative activity...
  24. pmc The Type II heat-labile enterotoxins LT-IIa and LT-IIb and their respective B pentamers differentially induce and regulate cytokine production in human monocytic cells
    George Hajishengallis
    Louisiana State University Health Sciences Center, Department of Microbiology, Immunology, and Parasitology, Center of Excellence in Oral and Craniofacial Biology, 1100 Florida Ave, Box 130, New Orleans 70119, USA
    Infect Immun 72:6351-8. 2004
    ..Moreover, the anti-inflammatory properties of the holotoxins may serve to suppress innate immunity and promote the survival of the pathogen...
  25. pmc Toll-like receptor 2 mediates cellular activation by the B subunits of type II heat-labile enterotoxins
    George Hajishengallis
    Department of Microbiology, Immunology, and Parasitology, Louisiana State University, Health Sciences Center, New Orleans, LA 70119, USA
    Infect Immun 73:1343-9. 2005
    ..TLR2-dependent activation of mononuclear cells by type II enterotoxin B subunits appears to be a novel mechanism whereby these molecules may exert their immunomodulatory and adjuvant activities...
  26. ncbi request reprint The A subunit of type IIb enterotoxin (LT-IIb) suppresses the proinflammatory potential of the B subunit and its ability to recruit and interact with TLR2
    Shuang Liang
    Center for Oral Health and Systemic Disease, Department of Periodontics, University of Louisville Health Sciences Center, Louisville, KY 40292, USA
    J Immunol 178:4811-9. 2007
    ..coli may suppress TLR-mediated innate immunity through the action of the enterotoxin A subunit...
  27. pmc Enterotoxin-based mucosal adjuvants alter antigen trafficking and induce inflammatory responses in the nasal tract
    Frederik W van Ginkel
    Department of Pathobiology, College of Veterinary Medicine, Auburn University, AL 36849, USA
    Infect Immun 73:6892-902. 2005
    ..Thus, redirection of TT is dependent on ADP-ribosyltransferase activity and GM1 binding and is associated with production of the inflammatory cytokine IL-6...

Research Grants1

  1. Mechanisms of Adjuvant Stimulation
    TERRY CONNELL; Fiscal Year: 2006
    ..The toxins will be used as molecular tools to investigate the mechanisms that regulate adjuvant activity. We will engineer a new class of recombinant toxins to evaluate as potential mucosal vaccines. ..