Frank L Conlon

Summary

Affiliation: University of North Carolina
Country: USA

Publications

  1. pmc Immunoisolation of protein complexes from Xenopus
    Frank L Conlon
    Department of Genetics, University of North Carolina, Chapel Hill, NC, USA
    Methods Mol Biol 917:369-90. 2012
  2. pmc The T-box family
    Val Wilson
    Department of Genetics, 220 Fordham Hall, University of North Carolina, Chapel Hill, NC 27599 3280, USA
    Genome Biol 3:REVIEWS3008. 2002
  3. pmc CASZ1 promotes vascular assembly and morphogenesis through the direct regulation of an EGFL7/RhoA-mediated pathway
    Marta S Charpentier
    McAllister Heart Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 3280, USA
    Dev Cell 25:132-43. 2013
  4. pmc The BMP pathway acts to directly regulate Tbx20 in the developing heart
    Elizabeth M Mandel
    University of North Carolina McAllister Heart Institute, UNC Chapel Hill, Chapel Hill, NC 27599, USA
    Development 137:1919-29. 2010
  5. pmc Vertebrate CASTOR is required for differentiation of cardiac precursor cells at the ventral midline
    Kathleen S Christine
    Carolina Cardiovascular Biology Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 3280, USA
    Dev Cell 14:616-23. 2008
  6. pmc SHP-2 is required for the maintenance of cardiac progenitors
    Yvette G Langdon
    Carolina Cardiovascular Biology Center, University of North Carolina, Chapel Hill, NC 27599, USA
    Development 134:4119-30. 2007
  7. pmc Proteomic profiling of cardiac tissue by isolation of nuclei tagged in specific cell types (INTACT)
    Nirav M Amin
    University of North Carolina McAllister Heart Institute, UNC Chapel Hill, Chapel Hill, NC 27599 3280, USA
    Development 141:962-73. 2014
  8. pmc Xtn3 is a developmentally expressed cardiac and skeletal muscle-specific novex-3 titin isoform
    Daniel D Brown
    Carolina Cardiovascular Biology Center, UNC Chapel Hill, Chapel Hill, NC 27599 7126, USA
    Gene Expr Patterns 6:913-8. 2006
  9. pmc TBX5 is required for embryonic cardiac cell cycle progression
    Sarah C Goetz
    Carolina Cardiovascular Biology Center, 5109 Neuroscience Research Building, Chapel Hill, NC 27599 7126, USA
    Development 133:2575-84. 2006
  10. pmc Small heat shock protein Hsp27 is required for proper heart tube formation
    Daniel D Brown
    Department of Biology, UNC Chapel Hill, Chapel Hill, North Carolina 27599 3280, USA
    Genesis 45:667-78. 2007

Research Grants

Collaborators

  • Joan M Taylor
  • John B Wallingford
  • Da Zhi Wang
  • Yvette G Langdon
  • Julius Aitsebaomo
  • Martin Moser
  • C Patterson
  • Chris Showell
  • Nirav M Amin
  • Panna Tandon
  • Marta S Charpentier
  • Kathleen S Christine
  • Daniel D Brown
  • Erin Kaltenbrun
  • Sarah C Goetz
  • Ileana M Cristea
  • Elizabeth M Mandel
  • Stephen Sojka
  • Devin Gibbs
  • Lauren M Kuchenbrod
  • Todd M Greco
  • Olav Binder
  • Jason T Doherty
  • Daniel DeWitt Brown
  • Claire E Trincot
  • Elitza K Koutleva
  • Val Wilson
  • Mei I Chung
  • Erin Osborne Nishimura
  • Maggie M Rigney
  • Tuo Li
  • Victoria L Bautch
  • Christopher E Slagle
  • Kerry M Dorr
  • Yana V Miteva
  • Leslie M Kennedy
  • Erich J Kushner
  • Kathleen Christine
  • Lauren Waldron
  • Thomas E Callis
  • Deborah Yelon
  • Sara R Marques
  • Christopher P Mack
  • Xin Xin I Zeng
  • Christopher Showell
  • Anna C Davis
  • Brenda M J Price
  • Shauna N Martz
  • Jim C Smith
  • Maria Pagratis
  • Brian A Parr

Detail Information

Publications34

  1. pmc Immunoisolation of protein complexes from Xenopus
    Frank L Conlon
    Department of Genetics, University of North Carolina, Chapel Hill, NC, USA
    Methods Mol Biol 917:369-90. 2012
    ..In addition, the procedures in this chapter can be extended to use with proteomic analysis of protein complexes as presented in the following chapter...
  2. pmc The T-box family
    Val Wilson
    Department of Genetics, 220 Fordham Hall, University of North Carolina, Chapel Hill, NC 27599 3280, USA
    Genome Biol 3:REVIEWS3008. 2002
    ....
  3. pmc CASZ1 promotes vascular assembly and morphogenesis through the direct regulation of an EGFL7/RhoA-mediated pathway
    Marta S Charpentier
    McAllister Heart Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 3280, USA
    Dev Cell 25:132-43. 2013
    ..Collectively, these studies show that CASZ1 is required to directly regulate an EGFL7/RhoA-mediated pathway to promote vertebrate vascular development...
  4. pmc The BMP pathway acts to directly regulate Tbx20 in the developing heart
    Elizabeth M Mandel
    University of North Carolina McAllister Heart Institute, UNC Chapel Hill, Chapel Hill, NC 27599, USA
    Development 137:1919-29. 2010
    ..Collectively, our findings define Tbx20 as a direct transcriptional target of the BMP/SMAD1 signaling pathway during cardiac maturation...
  5. pmc Vertebrate CASTOR is required for differentiation of cardiac precursor cells at the ventral midline
    Kathleen S Christine
    Carolina Cardiovascular Biology Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 3280, USA
    Dev Cell 14:616-23. 2008
    ..These midline-specific requirements for CST suggest the regulation of cardiomyocyte differentiation is regionalized along a dorsal-ventral axis and that this patterning occurs prior to heart tube formation...
  6. pmc SHP-2 is required for the maintenance of cardiac progenitors
    Yvette G Langdon
    Carolina Cardiovascular Biology Center, University of North Carolina, Chapel Hill, NC 27599, USA
    Development 134:4119-30. 2007
    ..Collectively, these results show that SHP-2 functions within the FGF/MAPK pathway to maintain survival of proliferating populations of cardiac progenitor cells...
  7. pmc Proteomic profiling of cardiac tissue by isolation of nuclei tagged in specific cell types (INTACT)
    Nirav M Amin
    University of North Carolina McAllister Heart Institute, UNC Chapel Hill, Chapel Hill, NC 27599 3280, USA
    Development 141:962-73. 2014
    ..Thus, by combining these technologies we are able to identify tissue-specific proteins that are expressed and required for normal vertebrate organ development. ..
  8. pmc Xtn3 is a developmentally expressed cardiac and skeletal muscle-specific novex-3 titin isoform
    Daniel D Brown
    Carolina Cardiovascular Biology Center, UNC Chapel Hill, Chapel Hill, NC 27599 7126, USA
    Gene Expr Patterns 6:913-8. 2006
    ..Finally, we demonstrate that Xtn3 transcripts are detectable in adult heart and muscle tissues...
  9. pmc TBX5 is required for embryonic cardiac cell cycle progression
    Sarah C Goetz
    Carolina Cardiovascular Biology Center, 5109 Neuroscience Research Building, Chapel Hill, NC 27599 7126, USA
    Development 133:2575-84. 2006
    ..Thus, these studies establish a role for TBX5 in regulating the progression of the cardiac cell cycle...
  10. pmc Small heat shock protein Hsp27 is required for proper heart tube formation
    Daniel D Brown
    Department of Biology, UNC Chapel Hill, Chapel Hill, North Carolina 27599 3280, USA
    Genesis 45:667-78. 2007
    ..Moreover, these defects are not associated with alterations in specification or differentiation. We have thus demonstrated a critical requirement for XHSP27 in developing cardiac and skeletal muscle tissues...
  11. pmc Focal adhesion kinase is essential for cardiac looping and multichamber heart formation
    Jason T Doherty
    Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Genesis 48:492-504. 2010
    ..Collectively our data imply that FAK plays an essential role in chamber outgrowth and looping morphogenesis likely stimulated by fibroblast growth factors (and possibly other) cardiotrophic factors...
  12. pmc Tbx5 and Tbx20 act synergistically to control vertebrate heart morphogenesis
    Daniel D Brown
    Department of Genetics, Fordham Hall, UNC Chapel Hill, Chapel Hill, NC 27599 3280, USA
    Development 132:553-63. 2005
    ....
  13. pmc Tcf21 regulates the specification and maturation of proepicardial cells
    Panna Tandon
    University of North Carolina McAllister Heart Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 3280, USA
    Development 140:2409-21. 2013
    ..Collectively, these studies lead us to propose that Tcf21 functions as a transcriptional repressor to regulate proepicardial cell specification and the correct formation of a mature epithelial epicardium...
  14. pmc Morpholino injection in Xenopus
    Panna Tandon
    Department of Genetics, UNC McAllister Heart Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
    Methods Mol Biol 843:29-46. 2012
    ....
  15. pmc Xenopus: An emerging model for studying congenital heart disease
    Erin Kaltenbrun
    University of North Carolina McAllister Heart Institute, Chapel Hill, NC 27599, USA
    Birth Defects Res A Clin Mol Teratol 91:495-510. 2011
    ..We also outline congenital heart conditions linked to cardiac genes that have been well studied in Xenopus and describe some emerging technologies that will further aid in the study of these complex syndromes...
  16. pmc T-box genes in early embryogenesis
    Chris Showell
    Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
    Dev Dyn 229:201-18. 2004
    ..Developmental Dynamics 229:201-218, 2004...
  17. pmc Congenital heart disease protein 5 associates with CASZ1 to maintain myocardial tissue integrity
    Stephen Sojka
    University of North Carolina McAllister Heart Institute, UNC Chapel Hill, Chapel Hill, NC 27599 3280, USA Department of Biology, UNC Chapel Hill, Chapel Hill, NC 27599 3280, USA
    Development 141:3040-9. 2014
    ..Collectively, these results establish a role for CHD5 and CASZ1 in the early stages of vertebrate cardiac development. ..
  18. pmc SHP-2 acts via ROCK to regulate the cardiac actin cytoskeleton
    Yvette Langdon
    University of North Carolina McAllister Heart Institute, UNC Chapel Hill, Chapel Hill, NC 27599 3280, USA
    Development 139:948-57. 2012
    ....
  19. pmc A Gro/TLE-NuRD corepressor complex facilitates Tbx20-dependent transcriptional repression
    Erin Kaltenbrun
    Departments of Biology and Genetics, University of North Carolina, Chapel Hill, North Carolina 27599, United States
    J Proteome Res 12:5395-409. 2013
    ..Together, these studies suggest a model in which Tbx20 associates with a Gro/TLE-NuRD repressor complex to prevent inappropriate gene activation within the forming heart. ..
  20. pmc BMPER, a novel endothelial cell precursor-derived protein, antagonizes bone morphogenetic protein signaling and endothelial cell differentiation
    Martin Moser
    Carolina Cardiovascular Biology Center, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Mol Cell Biol 23:5664-79. 2003
    ....
  21. pmc Cardiac progenitors and the embryonic cell cycle
    Sarah C Goetz
    Carolina Cardiovascular Biology Center, Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Cell Cycle 6:1974-81. 2007
    ..Thus, the exploration of cardiomyocyte cell cycle regulation, both during development as well as in the adult heart, promises to yield many exciting and important discoveries over the coming years...
  22. pmc Decoding development in Xenopus tropicalis
    Chris Showell
    Carolina Cardiovascular Biology Center and Department of Genetics, University of North Carolina, Chapel Hill, North Carolina, USA
    Genesis 45:418-26. 2007
    ....
  23. doi request reprint A distinct mechanism of vascular lumen formation in Xenopus requires EGFL7
    Marta S Charpentier
    University of North Carolina McAllister Heart Institute, UNC CH, Chapel Hill, North Carolina, United States of America Department of Genetics and Molecular Biology, UNC CH, Chapel Hill, North Carolina, United States of America
    PLoS ONE 10:e0116086. 2015
    ..Taken together, we demonstrate for the first time the mechanisms by which lumens are generated within the major vessels in Xenopus and implicate EGFL7 in modulating cell shape and cell-cell junctions to drive proper lumen morphogenesis. ..
  24. pmc RNA-seq in the tetraploid Xenopus laevis enables genome-wide insight in a classic developmental biology model organism
    Nirav M Amin
    University of North Carolina, McAllister Heart Institute, UNC Chapel Hill, Chapel Hill, NC 27599 3280, USA Department of Genetics, UNC Chapel Hill, Chapel Hill, NC 27599 3280, USA
    Methods 66:398-409. 2014
    ..For the purposes of this article, we use Xenopus laevis as the model organism to discuss uses of RNA-seq in an organism without a fully annotated genome sequence. ..
  25. pmc ROCKs cause SHP-wrecks and broken hearts
    Panna Tandon
    Department of Genetics, University of North Carolina, Chapel Hill, NC, USA
    Small Gtpases 3:209-12. 2012
    ....
  26. pmc Differential regulation of CASZ1 protein expression during cardiac and skeletal muscle development
    Nirav M Amin
    University of North Carolina McAllister Heart Institute, UNC Chapel Hill, Chapel Hill, North Carolina Department of Genetics, UNC Chapel Hill, Chapel Hill, North Carolina
    Dev Dyn 243:948-56. 2014
    ..However, expression of Casz1 mRNA is detected throughout the developing heart, suggesting the spatial regulation of CASZ1 occurs at the protein level. Relatively little is known about posttranscriptional regulation of Casz1 in the heart...
  27. pmc Cellular and molecular mechanisms underlying blood vessel lumen formation
    Marta S Charpentier
    McAllister Heart Institute, Departments of Biology and Genetics, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
    Bioessays 36:251-9. 2014
    ....
  28. pmc The Western Clawed Frog (Xenopus tropicalis): An Emerging Vertebrate Model for Developmental Genetics and Environmental Toxicology
    Chris Showell
    UNC McAllister Heart Institute, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    CSH Protoc 2009:pdb.emo131. 2009
    ....
  29. pmc Natural Mating and Tadpole Husbandry in the Western Clawed Frog Xenopus tropicalis
    Chris Showell
    UNC McAllister Heart Institute, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    CSH Protoc 2009:pdb.prot5292. 2009
    ..The method includes a technique for the anesthesia of frogs, which makes them easier to handle during injection of the hormone, reducing the likelihood of injury to the animals...
  30. pmc Egg Collection and In Vitro Fertilization of the Western Clawed Frog Xenopus tropicalis
    Chris Showell
    UNC McAllister Heart Institute, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    CSH Protoc 2009:pdb.prot5293. 2009
    ..g., for studies of gene expression), because it produces embryos that develop synchronously during early embryonic development. The typical yield of embryos ranges from several hundred to more than 1000 per fertilization...
  31. pmc Developmental expression of the Xenopus laevis Tbx20 orthologue
    Daniel DeWitt Brown
    Department of Genetics, 220 Fordham Hall, University of North Carolina, Chapel Hill, NC 27599 3280, USA
    Dev Genes Evol 212:604-7. 2003
    ..In addition, we also observe expression in the cement gland, the jugular vein, the lung bud, the cloacal aperture, rhombomeres 2, 4, 6 and 8, and in a subset of motor neurons...
  32. pmc Tissue Sampling and Genomic DNA Purification from the Western Clawed Frog Xenopus tropicalis
    Chris Showell
    UNC McAllister Heart Institute, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    CSH Protoc 2009:pdb.prot5294. 2009
    ..Typically, the DNA yield is sufficient for more than 100 standard 50-muL polymerase chain reactions (PCRs) (using 1-5 muL of DNA per reaction), so it can be used for genetic screening and mapping studies...
  33. pmc Developmental expression patterns of Tbx1, Tbx2, Tbx5, and Tbx20 in Xenopus tropicalis
    Chris Showell
    Carolina Cardiovascular Biology Center and Department of Genetics, University of North Carolina at Chapel Hill, North Carolina 27599 3280, USA
    Dev Dyn 235:1623-30. 2006
    ..Thus, X. tropicalis represents a potentially valuable vertebrate model in which to further investigate the functions of these genes through genetic approaches...

Research Grants2

  1. A Genetic Approach to Organogenesis in Xenopus
    Frank Conlon; Fiscal Year: 2006
    ..tropicalis. Two, determine the role of Shp-2 and Tbx5 in organ development through the generation of an allelic series of Shp-2 and Tbx5 in X. tropicalis. [unreadable] [unreadable]..
  2. Requirements for the FGF/MAPK in early heart development
    Frank Conlon; Fiscal Year: 2007
    ..abstract_text> ..