L Conforti

Summary

Affiliation: University of Cincinnati
Country: USA

Publications

  1. pmc Signalling during hypoxia in human T lymphocytes--critical role of the src protein tyrosine kinase p56Lck in the O2 sensitivity of Kv1.3 channels
    Peter Szigligeti
    Department of Internal Medicine, University of Cincinnati, Cincinnati, OH 45267 0585, USA
    J Physiol 573:357-70. 2006
  2. pmc Selective inhibition of a slow-inactivating voltage-dependent K+ channel in rat PC12 cells by hypoxia
    L Conforti
    Department of Molecular and Cellular Physiology, College of Medicine, University of Cincinnati, OH 45267 0576, USA
    J Physiol 502:293-305. 1997
  3. ncbi request reprint Mouse Na+: HCO3- cotransporter isoform NBC-3 (kNBC-3): cloning, expression, and renal distribution
    Z Wang
    Division of Nephrology, Department of Internal Medicine, University of Cincinnati, and the Veterans Affairs Medical Center, Cincinnati, Ohio, USA
    Kidney Int 59:1405-14. 2001
  4. ncbi request reprint Identification of a novel signal in the cytoplasmic tail of the Na+:HCO3- cotransporter NBC1 that mediates basolateral targeting
    Hong C Li
    Dept of Internal Medicine, Div of Nephrology and Hypertension, Univ of Cincinnati, 231 Albert Sabin Way, MSB G259, Cincinnati, OH 45267 0585, USA
    Am J Physiol Renal Physiol 292:F1245-55. 2007
  5. pmc Modulation of Kv1.3 channels by protein kinase A I in T lymphocytes is mediated by the disc large 1-tyrosine kinase Lck complex
    Zerrin Kuras
    Department of Internal Medicine, 231 Albert Sabin Way, University of Cincinnati, Cincinnati, OH 45267 0585, USA
    Am J Physiol Cell Physiol 302:C1504-12. 2012
  6. ncbi request reprint The pore region of the Kv1.2alpha subunit is an important component of recombinant Kv1.2 channel oxygen sensitivity
    Laura Conforti
    Division of Nephrology and Hypertension, Department of Internal Medicine, University of Cincinnati, 231 Albert Sabin Way, Cincinnati, OH 45267 0585, USA
    Biochem Biophys Res Commun 306:450-6. 2003
  7. pmc O2-sensitive K+ channels: role of the Kv1.2 -subunit in mediating the hypoxic response
    L Conforti
    Department of Molecular Physiology, Institute of Molecular Pharmacology and Biophysics, College of Medicine, University of Cincinnati, Ohio, USA
    J Physiol 524:783-93. 2000
  8. ncbi request reprint Hypoxia regulates expression and activity of Kv1.3 channels in T lymphocytes: a possible role in T cell proliferation
    Laura Conforti
    Departments of Internal Medicine, and Molecular and Cellular Physiology, University of Cincinnati, OH 45267, USA
    J Immunol 170:695-702. 2003
  9. pmc Adenosine modulates hypoxia-induced responses in rat PC12 cells via the A2A receptor
    S Kobayashi
    Department of Molecular and Cellular Physiology, University of Cincinnati, 231 Bethesda Avenue, PO Box 576, Cincinnati, OH 45267 576, USA
    J Physiol 508:95-107. 1998
  10. pmc Chronic hypoxia reduces adenosine A2A receptor-mediated inhibition of calcium current in rat PC12 cells via downregulation of protein kinase A
    S Kobayashi
    Department of Molecular and Cellular Physiology, University of Cincinnati, 231 Bethesda Avenue, PO Box 670576, Cincinnati, OH 45267 576, USA
    J Physiol 512:351-63. 1998

Research Grants

Collaborators

  • S Kobayashi
  • ROGER WORRELL
  • Z Wang
  • Qing Ma
  • H W Kim
  • M Soleimani
  • Koichi Takimoto
  • H Amlal
  • Olaf Pongs
  • C E Burnham
  • George D Thorne
  • DANIEL DEVOR
  • Maria F Czyzyk-Krzeska
  • D Gozal
  • Lisa Neumeier
  • Stella A Nicolaou
  • Hong C Li
  • Alexandra H Filipovich
  • Peter Szigligeti
  • Susan Molleran Lee
  • Jennifer R Robbins
  • Zerrin Kuras
  • Milan Petrovic
  • Vladimir Kucher
  • Adriana Zakrzewska
  • Jeffrey B Matthews
  • Massimo Tortarolo
  • Cristina Vanoni
  • Snezana Petrovic
  • Jie Xu
  • F Shama Fernando
  • P W Conrad
  • Ameet A Chimote
  • Scott M Gordon
  • Heather J Duncan
  • Anne Barbara Mongey
  • Shashi K Kant
  • Ashleigh Steckly
  • Emily Y Li
  • Youqing Peng
  • Claire Chougnet
  • Eugene Duke
  • Justin B Striet
  • Marc G Wathelet
  • Phillip O Schnell
  • Anna Hui
  • Grazia Pietrini
  • Andrew J Crossthwaite
  • Silvia Massari
  • Carla Perego
  • Robert J Williams
  • Jeremy P Spencer
  • Marcus Rattray
  • Holleh Husseinzadeh
  • Paolo Carrega
  • Marco Losa
  • Caterina Bendotti
  • Sharone Barone
  • Michael P Coleman
  • A David Smith
  • Sabrina Tosi
  • R T Rust
  • Y Yuan
  • K Seta
  • D Beitner-Johnson
  • D E Millhorn
  • R H Kim

Detail Information

Publications24

  1. pmc Signalling during hypoxia in human T lymphocytes--critical role of the src protein tyrosine kinase p56Lck in the O2 sensitivity of Kv1.3 channels
    Peter Szigligeti
    Department of Internal Medicine, University of Cincinnati, Cincinnati, OH 45267 0585, USA
    J Physiol 573:357-70. 2006
    ..3 currents in T lymphocytes. Our findings indicate that Lck is required for the acute response to hypoxia of human T lymphocytes as it is necessary to confer O(2) sensitivity on Kv 1.3 channels...
  2. pmc Selective inhibition of a slow-inactivating voltage-dependent K+ channel in rat PC12 cells by hypoxia
    L Conforti
    Department of Molecular and Cellular Physiology, College of Medicine, University of Cincinnati, OH 45267 0576, USA
    J Physiol 502:293-305. 1997
    ..6. These results indicate that the O2-sensitive K+ channel in PC12 cells is a 20 pS slow-inactivating K+ channel that is upregulated by hypoxia. This channel appears to belong to the Shaker subfamily of voltage-gated K+ channels...
  3. ncbi request reprint Mouse Na+: HCO3- cotransporter isoform NBC-3 (kNBC-3): cloning, expression, and renal distribution
    Z Wang
    Division of Nephrology, Department of Internal Medicine, University of Cincinnati, and the Veterans Affairs Medical Center, Cincinnati, Ohio, USA
    Kidney Int 59:1405-14. 2001
    ..The purpose of the present studies was to clone the mouse kNBC-3 and to examine its properties and expression in the kidney...
  4. ncbi request reprint Identification of a novel signal in the cytoplasmic tail of the Na+:HCO3- cotransporter NBC1 that mediates basolateral targeting
    Hong C Li
    Dept of Internal Medicine, Div of Nephrology and Hypertension, Univ of Cincinnati, 231 Albert Sabin Way, MSB G259, Cincinnati, OH 45267 0585, USA
    Am J Physiol Renal Physiol 292:F1245-55. 2007
    ..We propose that the FL motif in the COOH-terminal tail of NBC1 is essential for the targeting of NBC1 to the basolateral membrane but is distinct from the membrane-targeting di-leucine motif identified in other membrane proteins...
  5. pmc Modulation of Kv1.3 channels by protein kinase A I in T lymphocytes is mediated by the disc large 1-tyrosine kinase Lck complex
    Zerrin Kuras
    Department of Internal Medicine, 231 Albert Sabin Way, University of Cincinnati, Cincinnati, OH 45267 0585, USA
    Am J Physiol Cell Physiol 302:C1504-12. 2012
    ..3 channels in human T lymphocytes. This effect is mediated by Lck and Dlg1. We thus propose that the K(V)1.3/Dlg1/Lck complex is part of the membrane pathway that cAMP utilizes to regulate T-cell function...
  6. ncbi request reprint The pore region of the Kv1.2alpha subunit is an important component of recombinant Kv1.2 channel oxygen sensitivity
    Laura Conforti
    Division of Nephrology and Hypertension, Department of Internal Medicine, University of Cincinnati, 231 Albert Sabin Way, Cincinnati, OH 45267 0585, USA
    Biochem Biophys Res Commun 306:450-6. 2003
    ..Thus, the pore and its surrounding regions of Kv1.2 polypeptide confer its hypoxic inhibition. This response is independent on the redox modulation of methionine residues in this protein segment...
  7. pmc O2-sensitive K+ channels: role of the Kv1.2 -subunit in mediating the hypoxic response
    L Conforti
    Department of Molecular Physiology, Institute of Molecular Pharmacology and Biophysics, College of Medicine, University of Cincinnati, Ohio, USA
    J Physiol 524:783-93. 2000
    ..Hypoxia inhibited the Kv1.2 current only. These findings show that the KO2 channel in PC12 cells belongs to the Kv1 subfamily of K+ channels and that the Kv1.2 alpha-subunit is important in conferring O2 sensitivity to this channel...
  8. ncbi request reprint Hypoxia regulates expression and activity of Kv1.3 channels in T lymphocytes: a possible role in T cell proliferation
    Laura Conforti
    Departments of Internal Medicine, and Molecular and Cellular Physiology, University of Cincinnati, OH 45267, USA
    J Immunol 170:695-702. 2003
    ..Indeed, we herein present evidence showing that hypoxia selectively inhibits TCR-mediated proliferation and that this inhibition is associated with a decrease in Kv1.3 proteins...
  9. pmc Adenosine modulates hypoxia-induced responses in rat PC12 cells via the A2A receptor
    S Kobayashi
    Department of Molecular and Cellular Physiology, University of Cincinnati, 231 Bethesda Avenue, PO Box 576, Cincinnati, OH 45267 576, USA
    J Physiol 508:95-107. 1998
    ..We propose that this modulation serves to regulate membrane excitability in PC12 cells and possibly other oxygen-sensitive cells during hypoxia...
  10. pmc Chronic hypoxia reduces adenosine A2A receptor-mediated inhibition of calcium current in rat PC12 cells via downregulation of protein kinase A
    S Kobayashi
    Department of Molecular and Cellular Physiology, University of Cincinnati, 231 Bethesda Avenue, PO Box 670576, Cincinnati, OH 45267 576, USA
    J Physiol 512:351-63. 1998
    ..This mechanism may serve to reduce the negative feedback on ICa and [Ca2+]i by adenosine and therefore maintain enhanced membrane excitability of PC12 cells during long-term hypoxia...
  11. ncbi request reprint The molecular basis of O2-sensing and hypoxia tolerance in pheochromocytoma cells
    P W Conrad
    Department of Molecular and Cellular Physiology, University of Cincinnati, College of Medicine, P.O. Box 67-0576, Cincinnati, OH 45267-0576, USA
    Comp Biochem Physiol B Biochem Mol Biol 128:187-204. 2001
    ..Together, these results provide greater insight into the mechanisms by which cells sense and adapt to hypoxia...
  12. ncbi request reprint Hypoxic vasorelaxation inhibition by organ culture correlates with loss of Kv channels but not Ca(2+) channels
    George D Thorne
    Department of Molecular and Cellular Physiology, University of Cincinnati, College of Medicine, Cincinnati, Ohio 45267 0576, USA
    Am J Physiol Heart Circ Physiol 283:H247-53. 2002
    ..In conclusion, organ culture decreases expression of specific Kv channels. These changes are consistent with altered mechanisms of VSM contractility that may be involved in Ca(2+)-dependent pathways of hypoxia-induced vasodilation...
  13. ncbi request reprint Identification of a carboxyl-terminal motif essential for the targeting of Na+-HCO-3 cotransporter NBC1 to the basolateral membrane
    Hong C Li
    Department of Medicine, University of Cincinnati, Cincinnati, Ohio 45267, USA
    J Biol Chem 279:43190-7. 2004
    ....
  14. pmc Hypoxia modulates early events in T cell receptor-mediated activation in human T lymphocytes via Kv1.3 channels
    Jennifer R Robbins
    Department of Internal Medicine, 231 Albert Sabin Way, University of Cincinnati, Cincinnati, OH 45267 0585, USA
    J Physiol 564:131-43. 2005
    ..3 channels, as we find no influence of hypoxia on IKCa1 and CRAC channels. Our findings indicate that hypoxia modulates Ca2+ homeostasis in T cells via Kv1.3 channel inhibition and membrane depolarization...
  15. ncbi request reprint Expression of the Na+-HCO-3 cotransporter NBC4 in rat kidney and characterization of a novel NBC4 variant
    Jie Xu
    Division of Nephrology, Department of Medicine, University of Cincinnati, Ohio 45267 0585, USA
    Am J Physiol Renal Physiol 284:F41-50. 2003
    ..We propose that NBC4 is expressed in the thick ascending limb of the loop of Henle and mediates cellular HCO(3)(-) uptake in this segment...
  16. pmc Localization of Kv1.3 channels in the immunological synapse modulates the calcium response to antigen stimulation in T lymphocytes
    Stella A Nicolaou
    Department of Internal Medicine, University of Cincinnati, Cincinnati, OH 45267, USA
    J Immunol 183:6296-302. 2009
    ..This localization is important to control the amplitude of the Ca(2+) response, and disruption of this process can account for alterations of downstream Ca(2+)-dependent signaling events...
  17. pmc Hypoxia-activated metabolic pathway stimulates phosphorylation of p300 and CBP in oxygen-sensitive cells
    Adriana Zakrzewska
    Department of Genome Science, Division of Nephrology and Hypertension, University of Cincinnati, Ohio 45267 0505, USA
    J Neurochem 94:1288-96. 2005
    ..We propose that the NADH glycerol phosphate shuttle participates in generating a pool of ATP that serves either as a co-factor or a modulator of the kinases involved in the phosphorylation of p300/CBP during hypoxia...
  18. pmc The Ca(2+)-activated K(+) channel KCa3.1 compartmentalizes in the immunological synapse of human T lymphocytes
    Stella A Nicolaou
    Department of Internal Medicine, 231 Albert Sabin Way, University of Cincinnati, Cincinnati, OH 45267 0585, USA
    Am J Physiol Cell Physiol 292:C1431-9. 2007
    ..1 blocker TRAM-34 blocked Ca(2+) influx, but channel redistribution to the IS was not prevented. These results indicate that KCa3.1 channels are a part of the signaling complex that forms at the IS upon antigen presentation...
  19. ncbi request reprint Missense mutations in Na+:HCO3- cotransporter NBC1 show abnormal trafficking in polarized kidney cells: a basis of proximal renal tubular acidosis
    Hong C Li
    Dept of Medicine, University of Cincinnati, Cincinnati, OH 45267 0585, USA
    Am J Physiol Renal Physiol 289:F61-71. 2005
    ....
  20. pmc Differential calcium signaling and Kv1.3 trafficking to the immunological synapse in systemic lupus erythematosus
    Stella A Nicolaou
    Department of Internal Medicine, University of Cincinnati, OH 45267, USA
    Cell Calcium 47:19-28. 2010
    ..3 trafficking abnormalities contribute to the altered distribution in Ca(2+) signaling in SLE T cells. Overall these defects may explain in part the T cell hyperactivity and dysfunction documented in SLE patients...
  21. ncbi request reprint Increased internalisation and degradation of GLT-1 glial glutamate transporter in a cell model for familial amyotrophic lateral sclerosis (ALS)
    Cristina Vanoni
    Department of Pharmacology, School of Medicine, C E N D Center of Excellence on Neurodegenerative Diseases, University of Milan, IN CNR Cellular and Molecular Pharmacology Section, Via Vanvitelli 32, 20129 Milano, Italy
    J Cell Sci 117:5417-26. 2004
    ....
  22. ncbi request reprint Palmitoylation of KChIP splicing variants is required for efficient cell surface expression of Kv4.3 channels
    Koichi Takimoto
    Department of Environmental and Occupational Health, Graduate School of Public Health, University of Pittsburgh, 3343 Forbes Avenue, Pittsburgh, PA 15260, USA
    J Biol Chem 277:26904-11. 2002
    ..Mutating these cysteines reduces their plasma membrane localization and the enhancement of Kv4.3 current density. Thus, palmitoylation of the KChIP auxiliary subunits controls plasma membrane localization of their associated channels...
  23. ncbi request reprint Human homologue of a gene mutated in the slow Wallerian degeneration (C57BL/Wld(s)) mouse
    F Shama Fernando
    Department of Pharmacology, University of Oxford, Mansfield Road, Oxford OX1 3QT, UK
    Gene 284:23-9. 2002
    ..We have also determined the intron/exon structure of the gene, which will facilitate the screening of these exons for mutations in human neurodegenerative disorders...
  24. ncbi request reprint Expression of SOD1 G93A or wild-type SOD1 in primary cultures of astrocytes down-regulates the glutamate transporter GLT-1: lack of involvement of oxidative stress
    Massimo Tortarolo
    Biochemical Neuropharmacology Group, GKT Centre for Neuroscience Research, King s College London, Guy s Hospital Campus, London, UK
    J Neurochem 88:481-93. 2004
    ....

Research Grants7

  1. Hypoxia and Potassium Channel Activity in T Lymphocytes
    Laura Conforti; Fiscal Year: 2010
    ..We propose to study how the effects of those conditions on ion channels in the membrane of immune cells contribute to the failure of the immune system to destroy cancer cells. ..
  2. Hypoxia and Potassium Channel Activity in T Lymphocytes
    Laura Conforti; Fiscal Year: 2007
    ..Findings from the proposed studies will provide new insights into the molecular basis of the immune response in hypoxia and will further our understanding of the ionic mechanisms of hypoxia-mediated changes in T cell function. ..