Affiliation: University of Cincinnati
- Selective inhibition of a slow-inactivating voltage-dependent K+ channel in rat PC12 cells by hypoxiaL Conforti
Department of Molecular and Cellular Physiology, College of Medicine, University of Cincinnati, OH 45267 0576, USA
J Physiol 502:293-305. 1997..6. These results indicate that the O2-sensitive K+ channel in PC12 cells is a 20 pS slow-inactivating K+ channel that is upregulated by hypoxia. This channel appears to belong to the Shaker subfamily of voltage-gated K+ channels...
- Mouse Na+: HCO3- cotransporter isoform NBC-3 (kNBC-3): cloning, expression, and renal distributionZ Wang
Division of Nephrology, Department of Internal Medicine, University of Cincinnati, and the Veterans Affairs Medical Center, Cincinnati, Ohio, USA
Kidney Int 59:1405-14. 2001..The purpose of the present studies was to clone the mouse kNBC-3 and to examine its properties and expression in the kidney...
- Signalling during hypoxia in human T lymphocytes--critical role of the src protein tyrosine kinase p56Lck in the O2 sensitivity of Kv1.3 channelsPeter Szigligeti
Department of Internal Medicine, University of Cincinnati, Cincinnati, OH 45267 0585, USA
J Physiol 573:357-70. 2006..3 currents in T lymphocytes. Our findings indicate that Lck is required for the acute response to hypoxia of human T lymphocytes as it is necessary to confer O(2) sensitivity on Kv 1.3 channels...
- Identification of a novel signal in the cytoplasmic tail of the Na+:HCO3- cotransporter NBC1 that mediates basolateral targetingHong C Li
Dept of Internal Medicine, Div of Nephrology and Hypertension, Univ of Cincinnati, 231 Albert Sabin Way, MSB G259, Cincinnati, OH 45267 0585, USA
Am J Physiol Renal Physiol 292:F1245-55. 2007..We propose that the FL motif in the COOH-terminal tail of NBC1 is essential for the targeting of NBC1 to the basolateral membrane but is distinct from the membrane-targeting di-leucine motif identified in other membrane proteins...
- Modulation of Kv1.3 channels by protein kinase A I in T lymphocytes is mediated by the disc large 1-tyrosine kinase Lck complexZerrin Kuras
Department of Internal Medicine, 231 Albert Sabin Way, University of Cincinnati, Cincinnati, OH 45267 0585, USA
Am J Physiol Cell Physiol 302:C1504-12. 2012..3 channels in human T lymphocytes. This effect is mediated by Lck and Dlg1. We thus propose that the K(V)1.3/Dlg1/Lck complex is part of the membrane pathway that cAMP utilizes to regulate T-cell function...
- The pore region of the Kv1.2alpha subunit is an important component of recombinant Kv1.2 channel oxygen sensitivityLaura Conforti
Division of Nephrology and Hypertension, Department of Internal Medicine, University of Cincinnati, 231 Albert Sabin Way, Cincinnati, OH 45267 0585, USA
Biochem Biophys Res Commun 306:450-6. 2003..Thus, the pore and its surrounding regions of Kv1.2 polypeptide confer its hypoxic inhibition. This response is independent on the redox modulation of methionine residues in this protein segment...
- O2-sensitive K+ channels: role of the Kv1.2 -subunit in mediating the hypoxic responseL Conforti
Department of Molecular Physiology, Institute of Molecular Pharmacology and Biophysics, College of Medicine, University of Cincinnati, Ohio, USA
J Physiol 524:783-93. 2000..Hypoxia inhibited the Kv1.2 current only. These findings show that the KO2 channel in PC12 cells belongs to the Kv1 subfamily of K+ channels and that the Kv1.2 alpha-subunit is important in conferring O2 sensitivity to this channel...
- Hypoxia regulates expression and activity of Kv1.3 channels in T lymphocytes: a possible role in T cell proliferationLaura Conforti
Departments of Internal Medicine, and Molecular and Cellular Physiology, University of Cincinnati, OH 45267, USA
J Immunol 170:695-702. 2003..Indeed, we herein present evidence showing that hypoxia selectively inhibits TCR-mediated proliferation and that this inhibition is associated with a decrease in Kv1.3 proteins...
- Adenosine modulates hypoxia-induced responses in rat PC12 cells via the A2A receptorS Kobayashi
Department of Molecular and Cellular Physiology, University of Cincinnati, 231 Bethesda Avenue, PO Box 576, Cincinnati, OH 45267 576, USA
J Physiol 508:95-107. 1998..We propose that this modulation serves to regulate membrane excitability in PC12 cells and possibly other oxygen-sensitive cells during hypoxia...
- Chronic hypoxia reduces adenosine A2A receptor-mediated inhibition of calcium current in rat PC12 cells via downregulation of protein kinase AS Kobayashi
Department of Molecular and Cellular Physiology, University of Cincinnati, 231 Bethesda Avenue, PO Box 670576, Cincinnati, OH 45267 576, USA
J Physiol 512:351-63. 1998..This mechanism may serve to reduce the negative feedback on ICa and [Ca2+]i by adenosine and therefore maintain enhanced membrane excitability of PC12 cells during long-term hypoxia...
- The molecular basis of O2-sensing and hypoxia tolerance in pheochromocytoma cellsP W Conrad
Department of Molecular and Cellular Physiology, University of Cincinnati, College of Medicine, P.O. Box 67-0576, Cincinnati, OH 45267-0576, USA
Comp Biochem Physiol B Biochem Mol Biol 128:187-204. 2001..Together, these results provide greater insight into the mechanisms by which cells sense and adapt to hypoxia...
- Hypoxic vasorelaxation inhibition by organ culture correlates with loss of Kv channels but not Ca(2+) channelsGeorge D Thorne
Department of Molecular and Cellular Physiology, University of Cincinnati, College of Medicine, Cincinnati, Ohio 45267-0576, USA
Am J Physiol Heart Circ Physiol 283:H247-53. 2002..In conclusion, organ culture decreases expression of specific Kv channels. These changes are consistent with altered mechanisms of VSM contractility that may be involved in Ca(2+)-dependent pathways of hypoxia-induced vasodilation...
- Identification of a carboxyl-terminal motif essential for the targeting of Na+-HCO-3 cotransporter NBC1 to the basolateral membraneHong C Li
Department of Medicine, University of Cincinnati, Cincinnati, Ohio 45267, USA
J Biol Chem 279:43190-7. 2004....
- Hypoxia modulates early events in T cell receptor-mediated activation in human T lymphocytes via Kv1.3 channelsJennifer R Robbins
Department of Internal Medicine, 231 Albert Sabin Way, University of Cincinnati, Cincinnati, OH 45267-0585, USA
J Physiol 564:131-43. 2005..3 channels, as we find no influence of hypoxia on IKCa1 and CRAC channels. Our findings indicate that hypoxia modulates Ca2+ homeostasis in T cells via Kv1.3 channel inhibition and membrane depolarization...
- Expression of the Na+-HCO-3 cotransporter NBC4 in rat kidney and characterization of a novel NBC4 variantJie Xu
Division of Nephrology, Department of Medicine, University of Cincinnati, Ohio 45267 0585, USA
Am J Physiol Renal Physiol 284:F41-50. 2003..We propose that NBC4 is expressed in the thick ascending limb of the loop of Henle and mediates cellular HCO(3)(-) uptake in this segment...
- Localization of Kv1.3 channels in the immunological synapse modulates the calcium response to antigen stimulation in T lymphocytesStella A Nicolaou
Department of Internal Medicine, University of Cincinnati, Cincinnati, OH 45267, USA
J Immunol 183:6296-302. 2009..This localization is important to control the amplitude of the Ca(2+) response, and disruption of this process can account for alterations of downstream Ca(2+)-dependent signaling events...
- Hypoxia-activated metabolic pathway stimulates phosphorylation of p300 and CBP in oxygen-sensitive cellsAdriana Zakrzewska
Department of Genome Science, Division of Nephrology and Hypertension, University of Cincinnati, Ohio 45267 0505, USA
J Neurochem 94:1288-96. 2005..We propose that the NADH glycerol phosphate shuttle participates in generating a pool of ATP that serves either as a co-factor or a modulator of the kinases involved in the phosphorylation of p300/CBP during hypoxia...
- The Ca(2+)-activated K(+) channel KCa3.1 compartmentalizes in the immunological synapse of human T lymphocytesStella A Nicolaou
Department of Internal Medicine, 231 Albert Sabin Way, University of Cincinnati, Cincinnati, OH 45267 0585, USA
Am J Physiol Cell Physiol 292:C1431-9. 2007..1 blocker TRAM-34 blocked Ca(2+) influx, but channel redistribution to the IS was not prevented. These results indicate that KCa3.1 channels are a part of the signaling complex that forms at the IS upon antigen presentation...
- Missense mutations in Na+:HCO3- cotransporter NBC1 show abnormal trafficking in polarized kidney cells: a basis of proximal renal tubular acidosisHong C Li
Dept of Medicine, University of Cincinnati, Cincinnati, OH 45267 0585, USA
Am J Physiol Renal Physiol 289:F61-71. 2005....
- Differential calcium signaling and Kv1.3 trafficking to the immunological synapse in systemic lupus erythematosusStella A Nicolaou
Department of Internal Medicine, University of Cincinnati, OH 45267, USA
Cell Calcium 47:19-28. 2010..3 trafficking abnormalities contribute to the altered distribution in Ca(2+) signaling in SLE T cells. Overall these defects may explain in part the T cell hyperactivity and dysfunction documented in SLE patients...
- Increased internalisation and degradation of GLT-1 glial glutamate transporter in a cell model for familial amyotrophic lateral sclerosis (ALS)Cristina Vanoni
Department of Pharmacology, School of Medicine, C E N D Center of Excellence on Neurodegenerative Diseases, University of Milan, IN CNR Cellular and Molecular Pharmacology Section, Via Vanvitelli 32, 20129 Milano, Italy
J Cell Sci 117:5417-26. 2004....
- Palmitoylation of KChIP splicing variants is required for efficient cell surface expression of Kv4.3 channelsKoichi Takimoto
Department of Environmental and Occupational Health, Graduate School of Public Health, University of Pittsburgh, 3343 Forbes Avenue, Pittsburgh, PA 15260, USA
J Biol Chem 277:26904-11. 2002..Mutating these cysteines reduces their plasma membrane localization and the enhancement of Kv4.3 current density. Thus, palmitoylation of the KChIP auxiliary subunits controls plasma membrane localization of their associated channels...
- Human homologue of a gene mutated in the slow Wallerian degeneration (C57BL/Wld(s)) mouseF Shama Fernando
Department of Pharmacology, University of Oxford, Mansfield Road, Oxford OX1 3QT, UK
Gene 284:23-9. 2002..We have also determined the intron/exon structure of the gene, which will facilitate the screening of these exons for mutations in human neurodegenerative disorders...
- Expression of SOD1 G93A or wild-type SOD1 in primary cultures of astrocytes down-regulates the glutamate transporter GLT-1: lack of involvement of oxidative stressMassimo Tortarolo
Biochemical Neuropharmacology Group, GKT Centre for Neuroscience Research, King s College London, Guy s Hospital Campus, London, UK
J Neurochem 88:481-93. 2004....
- Hypoxia and Potassium Channel Activity in T LymphocytesLaura Conforti; Fiscal Year: 2010..We propose to study how the effects of those conditions on ion channels in the membrane of immune cells contribute to the failure of the immune system to destroy cancer cells. ..
- Hypoxia and Potassium Channel Activity in T LymphocytesLaura Conforti; Fiscal Year: 2007..Findings from the proposed studies will provide new insights into the molecular basis of the immune response in hypoxia and will further our understanding of the ionic mechanisms of hypoxia-mediated changes in T cell function. ..