K Collins

Summary

Affiliation: University of California
Country: USA

Publications

  1. ncbi request reprint Tetrahymena thermophila
    Kathleen Collins
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 3204, USA
    Curr Biol 15:R317-8. 2005
  2. ncbi request reprint Telomerase in the human organism
    Kathleen Collins
    Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California, CA 94720 3204, USA
    Oncogene 21:564-79. 2002
  3. pmc The reverse transcriptase component of the Tetrahymena telomerase ribonucleoprotein complex
    K Collins
    Department of Molecular and Cell Biology, 401 Barker Hall, University of California, Berkeley, CA 94720 3204, USA
    Proc Natl Acad Sci U S A 95:8485-90. 1998
  4. ncbi request reprint Ciliate telomerase biochemistry
    K Collins
    Department of Molecular and Cell Biology, University of California at Berkeley 94720 3204, USA
    Annu Rev Biochem 68:187-218. 1999
  5. ncbi request reprint Mammalian telomeres and telomerase
    K Collins
    Division of Biochemistry and Molecular Biology, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720 3204, USA
    Curr Opin Cell Biol 12:378-83. 2000
  6. ncbi request reprint Human telomerase activation requires two independent interactions between telomerase RNA and telomerase reverse transcriptase
    J R Mitchell
    Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA
    Mol Cell 6:361-71. 2000
  7. pmc Interaction of recombinant Tetrahymena telomerase proteins p80 and p95 with telomerase RNA and telomeric DNA substrates
    L Gandhi
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California 94720 3204, USA
    Genes Dev 12:721-33. 1998
  8. pmc RNA binding domain of telomerase reverse transcriptase
    C K Lai
    Division of Biochemistry and Molecular Biology, Department of Molecular and Cell Biology, University of California, Berkeley, California 94720-3204, USA
    Mol Cell Biol 21:990-1000. 2001
  9. ncbi request reprint A telomerase component is defective in the human disease dyskeratosis congenita
    J R Mitchell
    Department of Molecular and Cell Biology, University of California, Berkeley 94720 3204, USA
    Nature 402:551-5. 1999
  10. ncbi request reprint Requirements for the dGTP-dependent repeat addition processivity of recombinant Tetrahymena telomerase
    C D Hardy
    Division of Biochemistry and Molecular Biology, Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720-3204, USA
    J Biol Chem 276:4863-71. 2001

Collaborators

Detail Information

Publications51

  1. ncbi request reprint Tetrahymena thermophila
    Kathleen Collins
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 3204, USA
    Curr Biol 15:R317-8. 2005
  2. ncbi request reprint Telomerase in the human organism
    Kathleen Collins
    Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California, CA 94720 3204, USA
    Oncogene 21:564-79. 2002
    ..We discuss how our current perspectives on telomerase function could be applied to improving human health...
  3. pmc The reverse transcriptase component of the Tetrahymena telomerase ribonucleoprotein complex
    K Collins
    Department of Molecular and Cell Biology, 401 Barker Hall, University of California, Berkeley, CA 94720 3204, USA
    Proc Natl Acad Sci U S A 95:8485-90. 1998
    ..These experiments suggest a complexity of telomerase structure and function...
  4. ncbi request reprint Ciliate telomerase biochemistry
    K Collins
    Department of Molecular and Cell Biology, University of California at Berkeley 94720 3204, USA
    Annu Rev Biochem 68:187-218. 1999
    ..Telomerase components are being identified and beginning to be produced in recombinant form. This review focuses on the enzyme mechanism of telomerases from ciliate species, thus far the most intensively studied systems...
  5. ncbi request reprint Mammalian telomeres and telomerase
    K Collins
    Division of Biochemistry and Molecular Biology, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720 3204, USA
    Curr Opin Cell Biol 12:378-83. 2000
    ..Essential roles for telomerase in the sustained viability of cultured tumor cells and in the normal proliferative capacity of human somatic cells have been demonstrated...
  6. ncbi request reprint Human telomerase activation requires two independent interactions between telomerase RNA and telomerase reverse transcriptase
    J R Mitchell
    Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA
    Mol Cell 6:361-71. 2000
    ..Our results establish fundamental differences between vertebrate and ciliate telomerase ribonucleoprotein architectures and also suggest strategies for the pharmaceutical development of telomerase-based anticancer therapies...
  7. pmc Interaction of recombinant Tetrahymena telomerase proteins p80 and p95 with telomerase RNA and telomeric DNA substrates
    L Gandhi
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California 94720 3204, USA
    Genes Dev 12:721-33. 1998
    ....
  8. pmc RNA binding domain of telomerase reverse transcriptase
    C K Lai
    Division of Biochemistry and Molecular Biology, Department of Molecular and Cell Biology, University of California, Berkeley, California 94720-3204, USA
    Mol Cell Biol 21:990-1000. 2001
    ..Our results reveal at least one evolutionarily conserved molecular mechanism by which the telomerase reverse transcriptase is functionally specialized for obligate use of an internal RNA template...
  9. ncbi request reprint A telomerase component is defective in the human disease dyskeratosis congenita
    J R Mitchell
    Department of Molecular and Cell Biology, University of California, Berkeley 94720 3204, USA
    Nature 402:551-5. 1999
    ..The pathology of DKC is consistent with compromised telomerase function leading to a defect in telomere maintenance, which may limit the proliferative capacity of human somatic cells in epithelia and blood...
  10. ncbi request reprint Requirements for the dGTP-dependent repeat addition processivity of recombinant Tetrahymena telomerase
    C D Hardy
    Division of Biochemistry and Molecular Biology, Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720-3204, USA
    J Biol Chem 276:4863-71. 2001
    ..Finally, we show that a novel, nascent product DNA binding site establishes dGTP-dependent repeat addition processivity...
  11. pmc Biological and biochemical functions of RNA in the tetrahymena telomerase holoenzyme
    Doreen D Cunningham
    Department of Molecular and Cell Biology, 16 Barker Hall, University of California, Berkeley, CA 94720 3204, USA
    Mol Cell Biol 25:4442-54. 2005
    ..These findings demonstrate nontemplate functions of TER that are critical for the telomerase holoenzyme catalytic cycle and for proper telomere length maintenance in vivo...
  12. ncbi request reprint Human telomerase reverse transcriptase motifs required for elongation of a telomeric substrate
    Suzanne R Lee
    Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720 3204, USA
    J Biol Chem 278:52531-6. 2003
    ..Our findings suggest that non-template interactions of the telomerase ribonucleoprotein with telomeric DNA play a critical role in supporting telomerase function on its appropriate cellular substrates...
  13. pmc A novel RNA binding domain in tetrahymena telomerase p65 initiates hierarchical assembly of telomerase holoenzyme
    Catherine M O'Connor
    Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720 3204, USA
    Mol Cell Biol 26:2029-36. 2006
    ..These findings elucidate an extensive network of p65-TER recognition specificity and define a novel p65 RNA binding domain that initiates telomerase holoenyzme biogenesis...
  14. pmc The biogenesis and regulation of telomerase holoenzymes
    Kathleen Collins
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 3204, USA
    Nat Rev Mol Cell Biol 7:484-94. 2006
    ..Insights that have been gained into the cellular pathways for biogenesis and regulation of telomerase ribonucleoproteins raise new questions, particularly concerning the dynamic nature of this unique polymerase...
  15. pmc Physiological assembly and activity of human telomerase complexes
    Kathleen Collins
    Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720 3200, United States
    Mech Ageing Dev 129:91-8. 2008
    ..This review describes the current understanding of telomerase in humans, with particular focus on telomerase biogenesis and regulation in its cellular context...
  16. pmc Disease-associated human telomerase RNA variants show loss of function for telomere synthesis without dominant-negative interference
    Timothy M Errington
    Molecular and Cell Biology, University of California, Berkeley, CA 94720 3200, USA
    Mol Cell Biol 28:6510-20. 2008
    ..These findings support loss of function of telomerase RNA as a general mechanism of human disease...
  17. pmc Investigation of human telomerase holoenzyme assembly, activity, and processivity using disease-linked subunit variants
    Aaron R Robart
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 3200, USA
    J Biol Chem 285:4375-86. 2010
    ..Combined with additional hTR structure/function studies, our results establish a new resolution of insight into hTR structural requirements for hTR-TERT interaction and for the catalytic cycle of human telomerase holoenzyme...
  18. pmc An RPA-related sequence-specific DNA-binding subunit of telomerase holoenzyme is required for elongation processivity and telomere maintenance
    Bosun Min
    University of California, Berkeley, 94720 3200, USA
    Mol Cell 36:609-19. 2009
    ..The RPA-like p82 subunit binds sequence specifically to multiple telomeric repeats. These discoveries establish the existence of a telomerase holoenzyme processivity subunit with sequence-specific DNA binding...
  19. pmc A telomerase holoenzyme protein enhances telomerase RNA assembly with telomerase reverse transcriptase
    Ramadevi Prathapam
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA
    Nat Struct Mol Biol 12:252-7. 2005
    ..These findings reveal a pathway for telomerase holoenzyme biogenesis that preassembles TER for TERT recruitment...
  20. ncbi request reprint Structure and function of telomerase
    K Collins
    Department of Molecular and Cell Biology, University of California, Berkeley 94720 3204, USA
    Curr Opin Cell Biol 8:374-80. 1996
    ..In the past year, three areas of telomerase research have been particularly intense: assays of telomerase activity, isolation of telomerase components, and studies of the regulation of telomerase and telomere length in vivo...
  21. ncbi request reprint Subnuclear shuttling of human telomerase induced by transformation and DNA damage
    Judy M Y Wong
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 3204, USA
    Nat Cell Biol 4:731-6. 2002
    ..These findings show that transformation and DNA damage have opposite effects on the cellular regulation of active telomerase, affecting the enzyme's access to both telomeric and nontelomeric substrates...
  22. pmc Two purified domains of telomerase reverse transcriptase reconstitute sequence-specific interactions with RNA
    Catherine M O'Connor
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 3204, USA
    J Biol Chem 280:17533-9. 2005
    ..Our findings demonstrate telomerase functional specialization by an elaborate ribonucleoprotein architecture physically separable from the active site...
  23. pmc Holoenzyme proteins required for the physiological assembly and activity of telomerase
    Keren L Witkin
    Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720 3204, USA
    Genes Dev 18:1107-18. 2004
    ..These findings demonstrate that telomerase holoenzyme proteins other than TERT play critical roles in RNP biogenesis and function...
  24. pmc Telomerase recognizes its template by using an adjacent RNA motif
    Michael C Miller
    Department of Molecular and Cell Biology, University of California, 401 Barker Hall, Berkeley, CA 94720 3204, USA
    Proc Natl Acad Sci U S A 99:6585-90. 2002
    ..Our findings reveal molecular requirements for template selection by telomerase and physically resolve templating from other RNA functions in catalysis...
  25. pmc Telomerase RNA level limits telomere maintenance in X-linked dyskeratosis congenita
    Judy M Y Wong
    Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720, USA
    Genes Dev 20:2848-58. 2006
    ..These findings support the significance of telomerase deficiency in the pathology of X-linked DC...
  26. pmc Specificity and stoichiometry of subunit interactions in the human telomerase holoenzyme assembled in vivo
    Emily D Egan
    Department of Molecular and Cell Biology, 331 LSA, University of California, Berkeley, CA 94720 3200, USA
    Mol Cell Biol 30:2775-86. 2010
    ..This study uncovers unexpected similarities but also differences between telomerase and other H/ACA RNPs that allow a unique specificity of telomerase biogenesis and regulation...
  27. pmc Human telomerase and Cajal body ribonucleoproteins share a unique specificity of Sm protein association
    Dragony Fu
    Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720, USA
    Genes Dev 20:531-6. 2006
    ..We show that a Cajal body RNA localization motif determines this specificity. These discoveries expand the cellular repertoire of Sm protein assemblies and their involvement in ribonucleoprotein localization and function...
  28. pmc Purification of human telomerase complexes identifies factors involved in telomerase biogenesis and telomere length regulation
    Dragony Fu
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720 3200, USA
    Mol Cell 28:773-85. 2007
    ..Our findings suggest that endogenous human telomerase complexes are more heterogeneous than those of single-celled eukaryotes, have predominantly shared rather than telomerase-specific proteins, and make numerous regulatory interactions...
  29. ncbi request reprint Distinct biogenesis pathways for human telomerase RNA and H/ACA small nucleolar RNAs
    Dragony Fu
    Department of Molecular and Cell Biology, 401 Barker Hall, University of California at Berkeley, 97420, USA
    Mol Cell 11:1361-72. 2003
    ..Our results reveal differences between the biogenesis of hTR and other H/ACA-motif RNAs and demonstrate distinct mechanisms of telomerase inhibition in human disease...
  30. pmc Ciliate telomerase RNA loop IV nucleotides promote hierarchical RNP assembly and holoenzyme stability
    Aaron R Robart
    Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720 3200, USA
    RNA 16:563-71. 2010
    ..Our results point to a unifying model of a conformational activation role for this TER motif in the telomerase RNP enzyme...
  31. ncbi request reprint Telomere maintenance and disease
    Judy M Y Wong
    Department of Molecular and Cell Biology, University of California at Berkeley, 401 Barker Hall, Berkeley, CA 94720 3204, USA
    Lancet 362:983-8. 2003
    ..We review the evidence for premature telomere attrition in proliferative deficiencies of the human haemopoietic system, and discuss the potential use of telomerase activation in telomere-restorative gene therapy...
  32. pmc Multiple mechanisms for elongation processivity within the reconstituted tetrahymena telomerase holoenzyme
    Bosun Min
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 3200, USA
    J Biol Chem 285:16434-43. 2010
    ..Overall, our findings reveal multiple mechanisms and multiple surfaces of protein-DNA and protein-protein interaction that give rise to elongation processivity in the synthesis of a single-stranded nucleic acid product...
  33. ncbi request reprint Telomerase RNA deficiency in peripheral blood mononuclear cells in X-linked dyskeratosis congenita
    Judy M Y Wong
    Department of Molecular and Cell Biology, University of California, Berkeley 94720 3204, USA
    Hum Genet 115:448-55. 2004
    ..Our findings support the clinical significance of telomerase deficiency and encourage the use of telomerase activation as a disease therapy...
  34. pmc Biochemical properties of Trypanosoma cruzi telomerase
    Denise P Muñoz
    Department of Molecular and Cell Biology, 16 Barker Hall, University of California at Berkeley, Berkeley, CA 94720 3204, USA
    Nucleic Acids Res 32:5214-22. 2004
    ..These results expand our understanding of telomerase enzymology and should encourage the development of parasite-specific telomerase inhibitors as a method for disease therapy...
  35. ncbi request reprint Starvation-induced cleavage of the tRNA anticodon loop in Tetrahymena thermophila
    Suzanne R Lee
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 3204, USA
    J Biol Chem 280:42744-9. 2005
    ..Our findings suggest that anticodon loop cleavage may reduce the accumulation of uncharged tRNAs as part of a specific response induced by amino acid starvation...
  36. pmc Sequence, biogenesis, and function of diverse small RNA classes bound to the Piwi family proteins of Tetrahymena thermophila
    Mary T Couvillion
    Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720, USA
    Genes Dev 23:2016-32. 2009
    ..These studies greatly expand the structural and functional repertoire of endogenous sRNAs and RNPs...
  37. pmc Effect of pseudouridylation on the structure and activity of the catalytically essential P6.1 hairpin in human telomerase RNA
    Nak Kyoon Kim
    Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, USA
    Nucleic Acids Res 38:6746-56. 2010
    ..1 slightly attenuates telomerase activity but slightly increases processivity in vitro. Our results suggest that Ψs could have a subtle influence on human telomerase activity via impact on TER-TERT or TER-TER interactions...
  38. pmc Roles for RNA in telomerase nucleotide and repeat addition processivity
    Cary K Lai
    Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, CA 94720, USA
    Mol Cell 11:1673-83. 2003
    ..These findings demonstrate RNA elements with roles in telomerase elongation processivity that are distinct from RNA elements that specify the internal template...
  39. pmc Positive and negative regulation of Tetrahymena telomerase holoenzyme
    Keren L Witkin
    Department of Molecular and Cell Biology, University of California at Berkeley, CA 94720 3204, USA
    Mol Cell Biol 27:2074-83. 2007
    ..These studies reveal additional complexity in the positive and negative regulation of Tetrahymena telomerase function...
  40. pmc RNA-based affinity purification reveals 7SK RNPs with distinct composition and regulation
    J Robert Hogg
    Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720 3200, USA
    RNA 13:868-80. 2007
    ..Our results establish the utility of a generalizable RNA-based RNP affinity purification method and provide insight into 7SK RNP dynamics...
  41. pmc Constitutive secretion in Tetrahymena thermophila
    Catherine L Madinger
    Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720 3200, USA
    Eukaryot Cell 9:674-81. 2010
    ..Our work provides the first comprehensive analysis of secreted proteins in T. thermophila and establishes the groundwork for future studies of constitutive protein secretion biology and biotechnology in ciliates...
  42. pmc Template boundary definition in Tetrahymena telomerase
    Cary K Lai
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 3204, USA
    Genes Dev 16:415-20. 2002
    ..Our results indicate that sequence-specific interaction of a telomerase RNA element with the TERT RNA binding domain, not the active site motifs, defines the template boundary...
  43. pmc A single RNA-dependent RNA polymerase assembles with mutually exclusive nucleotidyl transferase subunits to direct different pathways of small RNA biogenesis
    Suzanne Rebecca Lee
    Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720 3200, USA
    RNA 15:1363-74. 2009
    ..The biochemical and biological phenotypes of RDRC subunit disruption reveal a previously unanticipated complexity of Rdr-dependent sRNA biogenesis in vivo...
  44. pmc Human Y5 RNA specializes a Ro ribonucleoprotein for 5S ribosomal RNA quality control
    J Robert Hogg
    Department of Molecular and Cell Biology, University of California at Berkely, Berkeley, California 94720, USA
    Genes Dev 21:3067-72. 2007
    ..These findings reveal a contribution of Y5 to 5S surveillance and suggest that interactions between Ro-Y5 and L5-5S RNPs establish 5S RNA as a target of quality control...
  45. ncbi request reprint Physical and functional coupling of RNA-dependent RNA polymerase and Dicer in the biogenesis of endogenous siRNAs
    Suzanne R Lee
    Department of Molecular and Cell Biology, University of California Berkeley, 142 Life Sciences Addition 3200, Berkeley, California 94720 3200, USA
    Nat Struct Mol Biol 14:604-10. 2007
    ..These findings suggest that sRNA strand bias arises from Dcr2 processing polarity, conferred by physical and functional coupling of RDRC and Dicer enzymes...
  46. pmc Two classes of endogenous small RNAs in Tetrahymena thermophila
    Suzanne R Lee
    Department of Molecular and Cell Biology, University of California at Berkeley, 94720 3204, USA
    Genes Dev 20:28-33. 2006
    ..These findings reveal the existence of distinct endogenous RNAi pathways in the unicellular T. thermophila, a complexity previously demonstrated only in multicellular organisms...
  47. pmc The telomerase reverse transcriptase regulates chromatin state and DNA damage responses
    Kenkichi Masutomi
    Department of Medical Oncology and Biostatistical Science, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 102:8222-7. 2005
    ..Cells lacking hTERT exhibit increased radiosensitivity, diminished capacity for DNA repair, and fragmented chromosomes, demonstrating that loss of hTERT impairs the DNA damage response...
  48. pmc Structural study of elements of Tetrahymena telomerase RNA stem-loop IV domain important for function
    Rebecca J Richards
    Department of Molecular Biology and Biochemistry, University of South Bohemia, Czech Republic
    RNA 12:1475-85. 2006
    ..The results suggest that during holoenzyme assembly the protein p65 recognizes a bend in stem IV, and this binding to central stem IV helps to position the structured loop IV for interaction with TERT and other region(s) of TER...
  49. pmc Stepwise protein-mediated RNA folding directs assembly of telomerase ribonucleoprotein
    Michael D Stone
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Nature 446:458-61. 2007
    ..These results identify the RNA folding pathway during telomerase biogenesis and define the mechanism of action for an essential telomerase holoenzyme protein...
  50. pmc Macronuclear genome sequence of the ciliate Tetrahymena thermophila, a model eukaryote
    Jonathan A Eisen
    The Institute for Genomic Research, Rockville, Maryland, United States of America
    PLoS Biol 4:e286. 2006
    ..thermophila an ideal model for functional genomic studies to address biological, biomedical, and biotechnological questions of fundamental importance...
  51. pmc High-resolution physical and functional mapping of the template adjacent DNA binding site in catalytically active telomerase
    Erez Romi
    Department of Biology, Technion Israel Institute of Technology, Haifa 32000, Israel
    Proc Natl Acad Sci U S A 104:8791-6. 2007
    ..Our data also suggest a movement of the TERT active site relative to the template-adjacent single-stranded DNA binding site within a cycle of repeat synthesis...