Research Topics
Species | K CollinsSummaryAffiliation: University of California Country: USA Publications
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Publications
Tetrahymena thermophilaKathleen Collins
Department of Molecular and Cell Biology, University of California, Berkeley, California 94720-3204, USA
Curr Biol 15:R317-8. 2005- Telomerase in the human organismKathleen Collins
Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California, CA 94720 3204, USA
Oncogene 21:564-79. 2002..We discuss how our current perspectives on telomerase function could be applied to improving human health... 
The reverse transcriptase component of the Tetrahymena telomerase ribonucleoprotein complexK Collins
Department of Molecular and Cell Biology, 401 Barker Hall, University of California, Berkeley, CA 94720 3204, USA
Proc Natl Acad Sci U S A 95:8485-90. 1998..These experiments suggest a complexity of telomerase structure and function...- Ciliate telomerase biochemistryK Collins
Department of Molecular and Cell Biology, University of California at Berkeley 94720 3204, USA
Annu Rev Biochem 68:187-218. 1999..Telomerase components are being identified and beginning to be produced in recombinant form. This review focuses on the enzyme mechanism of telomerases from ciliate species, thus far the most intensively studied systems... - Mammalian telomeres and telomeraseK Collins
Division of Biochemistry and Molecular Biology, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720 3204, USA
Curr Opin Cell Biol 12:378-83. 2000..Essential roles for telomerase in the sustained viability of cultured tumor cells and in the normal proliferative capacity of human somatic cells have been demonstrated... - Human telomerase activation requires two independent interactions between telomerase RNA and telomerase reverse transcriptaseJ R Mitchell
Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA
Mol Cell 6:361-71. 2000..Our results establish fundamental differences between vertebrate and ciliate telomerase ribonucleoprotein architectures and also suggest strategies for the pharmaceutical development of telomerase-based anticancer therapies... - Interaction of recombinant Tetrahymena telomerase proteins p80 and p95 with telomerase RNA and telomeric DNA substratesL Gandhi
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California 94720 3204, USA
Genes Dev 12:721-33. 1998.... - RNA binding domain of telomerase reverse transcriptaseC K Lai
Division of Biochemistry and Molecular Biology, Department of Molecular and Cell Biology, University of California, Berkeley, California 94720-3204, USA
Mol Cell Biol 21:990-1000. 2001..Our results reveal at least one evolutionarily conserved molecular mechanism by which the telomerase reverse transcriptase is functionally specialized for obligate use of an internal RNA template... - A telomerase component is defective in the human disease dyskeratosis congenitaJ R Mitchell
Department of Molecular and Cell Biology, University of California, Berkeley 94720 3204, USA
Nature 402:551-5. 1999..The pathology of DKC is consistent with compromised telomerase function leading to a defect in telomere maintenance, which may limit the proliferative capacity of human somatic cells in epithelia and blood... - Requirements for the dGTP-dependent repeat addition processivity of recombinant Tetrahymena telomeraseC D Hardy
Division of Biochemistry and Molecular Biology, Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720-3204, USA
J Biol Chem 276:4863-71. 2001..Finally, we show that a novel, nascent product DNA binding site establishes dGTP-dependent repeat addition processivity... - Biological and biochemical functions of RNA in the tetrahymena telomerase holoenzymeDoreen D Cunningham
Department of Molecular and Cell Biology, 16 Barker Hall, University of California, Berkeley, CA 94720-3204, USA
Mol Cell Biol 25:4442-54. 2005..These findings demonstrate nontemplate functions of TER that are critical for the telomerase holoenzyme catalytic cycle and for proper telomere length maintenance in vivo... - Human telomerase reverse transcriptase motifs required for elongation of a telomeric substrateSuzanne R Lee
Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720-3204, USA
J Biol Chem 278:52531-6. 2003..Our findings suggest that non-template interactions of the telomerase ribonucleoprotein with telomeric DNA play a critical role in supporting telomerase function on its appropriate cellular substrates... - A novel RNA binding domain in tetrahymena telomerase p65 initiates hierarchical assembly of telomerase holoenzymeKathleen Collins
Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720-3204, USA
Mol Cell Biol 26:2029-36. 2006..These findings elucidate an extensive network of p65-TER recognition specificity and define a novel p65 RNA binding domain that initiates telomerase holoenyzme biogenesis... - The biogenesis and regulation of telomerase holoenzymesKathleen Collins
Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 3204, USA
Nat Rev Mol Cell Biol 7:484-94. 2006..Insights that have been gained into the cellular pathways for biogenesis and regulation of telomerase ribonucleoproteins raise new questions, particularly concerning the dynamic nature of this unique polymerase... - Physiological assembly and activity of human telomerase complexesKathleen Collins
Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720 3200, United States
Mech Ageing Dev 129:91-8. 2008..This review describes the current understanding of telomerase in humans, with particular focus on telomerase biogenesis and regulation in its cellular context... - Disease-associated human telomerase RNA variants show loss of function for telomere synthesis without dominant-negative interferenceTimothy M Errington
Molecular and Cell Biology, University of California, Berkeley, CA 94720 3200, USA
Mol Cell Biol 28:6510-20. 2008..These findings support loss of function of telomerase RNA as a general mechanism of human disease... - Investigation of human telomerase holoenzyme assembly, activity, and processivity using disease-linked subunit variantsAaron R Robart
Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 3200, USA
J Biol Chem 285:4375-86. 2010..Combined with additional hTR structure/function studies, our results establish a new resolution of insight into hTR structural requirements for hTR-TERT interaction and for the catalytic cycle of human telomerase holoenzyme... - An RPA-related sequence-specific DNA-binding subunit of telomerase holoenzyme is required for elongation processivity and telomere maintenanceBosun Min
University of California, Berkeley, 94720 3200, USA
Mol Cell 36:609-19. 2009..The RPA-like p82 subunit binds sequence specifically to multiple telomeric repeats. These discoveries establish the existence of a telomerase holoenzyme processivity subunit with sequence-specific DNA binding... - A telomerase holoenzyme protein enhances telomerase RNA assembly with telomerase reverse transcriptaseRamadevi Prathapam
Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA
Nat Struct Mol Biol 12:252-7. 2005..These findings reveal a pathway for telomerase holoenzyme biogenesis that preassembles TER for TERT recruitment... - Structure and function of telomeraseK Collins
Department of Molecular and Cell Biology, University of California, Berkeley 94720 3204, USA
Curr Opin Cell Biol 8:374-80. 1996..In the past year, three areas of telomerase research have been particularly intense: assays of telomerase activity, isolation of telomerase components, and studies of the regulation of telomerase and telomere length in vivo... - Subnuclear shuttling of human telomerase induced by transformation and DNA damageJudy M Y Wong
Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 3204, USA
Nat Cell Biol 4:731-6. 2002..These findings show that transformation and DNA damage have opposite effects on the cellular regulation of active telomerase, affecting the enzyme's access to both telomeric and nontelomeric substrates... - Two purified domains of telomerase reverse transcriptase reconstitute sequence-specific interactions with RNACary K Lai
Department of Molecular and Cell Biology, University of California, Berkeley, California 94720-3204, USA
J Biol Chem 280:17533-9. 2005..Our findings demonstrate telomerase functional specialization by an elaborate ribonucleoprotein architecture physically separable from the active site... - Holoenzyme proteins required for the physiological assembly and activity of telomeraseKeren L Witkin
Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720-3204, USA
Genes Dev 18:1107-18. 2004..These findings demonstrate that telomerase holoenzyme proteins other than TERT play critical roles in RNP biogenesis and function... - Telomerase recognizes its template by using an adjacent RNA motifMichael C Miller
Department of Molecular and Cell Biology, University of California, 401 Barker Hall, Berkeley, CA 94720-3204, USA
Proc Natl Acad Sci U S A 99:6585-90. 2002..Our findings reveal molecular requirements for template selection by telomerase and physically resolve templating from other RNA functions in catalysis... - Telomerase RNA level limits telomere maintenance in X-linked dyskeratosis congenitaJudy M Y Wong
Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720, USA
Genes Dev 20:2848-58. 2006..These findings support the significance of telomerase deficiency in the pathology of X-linked DC... - Specificity and stoichiometry of subunit interactions in the human telomerase holoenzyme assembled in vivoEmily D Egan
Department of Molecular and Cell Biology, 331 LSA, University of California, Berkeley, CA 94720 3200, USA
Mol Cell Biol 30:2775-86. 2010..This study uncovers unexpected similarities but also differences between telomerase and other H/ACA RNPs that allow a unique specificity of telomerase biogenesis and regulation... - Human telomerase and Cajal body ribonucleoproteins share a unique specificity of Sm protein associationDragony Fu
Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720, USA
Genes Dev 20:531-6. 2006..We show that a Cajal body RNA localization motif determines this specificity. These discoveries expand the cellular repertoire of Sm protein assemblies and their involvement in ribonucleoprotein localization and function... - Purification of human telomerase complexes identifies factors involved in telomerase biogenesis and telomere length regulationDragony Fu
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720 3200, USA
Mol Cell 28:773-85. 2007..Our findings suggest that endogenous human telomerase complexes are more heterogeneous than those of single-celled eukaryotes, have predominantly shared rather than telomerase-specific proteins, and make numerous regulatory interactions... - Distinct biogenesis pathways for human telomerase RNA and H/ACA small nucleolar RNAsDragony Fu
Department of Molecular and Cell Biology, 401 Barker Hall, University of California at Berkeley, 97420, USA
Mol Cell 11:1361-72. 2003..Our results reveal differences between the biogenesis of hTR and other H/ACA-motif RNAs and demonstrate distinct mechanisms of telomerase inhibition in human disease... - Ciliate telomerase RNA loop IV nucleotides promote hierarchical RNP assembly and holoenzyme stabilityAaron R Robart
Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720 3200, USA
RNA 16:563-71. 2010..Our results point to a unifying model of a conformational activation role for this TER motif in the telomerase RNP enzyme... - Telomere maintenance and diseaseJudy M Y Wong
Department of Molecular and Cell Biology, University of California at Berkeley, 401 Barker Hall, Berkeley, CA 94720-3204, USA
Lancet 362:983-8. 2003..We review the evidence for premature telomere attrition in proliferative deficiencies of the human haemopoietic system, and discuss the potential use of telomerase activation in telomere-restorative gene therapy... - Multiple mechanisms for elongation processivity within the reconstituted tetrahymena telomerase holoenzymeBosun Min
Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 3200, USA
J Biol Chem 285:16434-43. 2010..Overall, our findings reveal multiple mechanisms and multiple surfaces of protein-DNA and protein-protein interaction that give rise to elongation processivity in the synthesis of a single-stranded nucleic acid product... - Telomerase RNA deficiency in peripheral blood mononuclear cells in X-linked dyskeratosis congenitaJudy M Y Wong
Department of Molecular and Cell Biology, University of California, Berkeley 94720-3204, USA
Hum Genet 115:448-55. 2004..Our findings support the clinical significance of telomerase deficiency and encourage the use of telomerase activation as a disease therapy... - Biochemical properties of Trypanosoma cruzi telomeraseDenise P Muñoz
Department of Molecular and Cell Biology, 16 Barker Hall, University of California at Berkeley, Berkeley, CA 94720 3204, USA
Nucleic Acids Res 32:5214-22. 2004..These results expand our understanding of telomerase enzymology and should encourage the development of parasite-specific telomerase inhibitors as a method for disease therapy... - Starvation-induced cleavage of the tRNA anticodon loop in Tetrahymena thermophilaSuzanne R Lee
Department of Molecular and Cell Biology, University of California, Berkeley, California 94720-3204, USA
J Biol Chem 280:42744-9. 2005..Our findings suggest that anticodon loop cleavage may reduce the accumulation of uncharged tRNAs as part of a specific response induced by amino acid starvation... - Sequence, biogenesis, and function of diverse small RNA classes bound to the Piwi family proteins of Tetrahymena thermophilaMary T Couvillion
Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720, USA
Genes Dev 23:2016-32. 2009..These studies greatly expand the structural and functional repertoire of endogenous sRNAs and RNPs... - Effect of pseudouridylation on the structure and activity of the catalytically essential P6.1 hairpin in human telomerase RNANak Kyoon Kim
Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, USA
Nucleic Acids Res 38:6746-56. 2010..1 slightly attenuates telomerase activity but slightly increases processivity in vitro. Our results suggest that Ψs could have a subtle influence on human telomerase activity via impact on TER-TERT or TER-TER interactions... - Roles for RNA in telomerase nucleotide and repeat addition processivityCary K Lai
Department of Molecular and Cell Biology, University of California-Berkeley, Berkeley, CA 94720, USA
Mol Cell 11:1673-83. 2003..These findings demonstrate RNA elements with roles in telomerase elongation processivity that are distinct from RNA elements that specify the internal template... - Positive and negative regulation of Tetrahymena telomerase holoenzymeKeren L Witkin
Department of Molecular and Cell Biology, University of California at Berkeley, CA 94720 3204, USA
Mol Cell Biol 27:2074-83. 2007..These studies reveal additional complexity in the positive and negative regulation of Tetrahymena telomerase function... - RNA-based affinity purification reveals 7SK RNPs with distinct composition and regulationJ Robert Hogg
Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720 3200, USA
RNA 13:868-80. 2007..Our results establish the utility of a generalizable RNA-based RNP affinity purification method and provide insight into 7SK RNP dynamics... - Constitutive secretion in Tetrahymena thermophilaCatherine L Madinger
Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720 3200, USA
Eukaryot Cell 9:674-81. 2010..Our work provides the first comprehensive analysis of secreted proteins in T. thermophila and establishes the groundwork for future studies of constitutive protein secretion biology and biotechnology in ciliates... - Template boundary definition in Tetrahymena telomeraseCary K Lai
Department of Molecular and Cell Biology, University of California, Berkeley, California 94720-3204, USA
Genes Dev 16:415-20. 2002..Our results indicate that sequence-specific interaction of a telomerase RNA element with the TERT RNA binding domain, not the active site motifs, defines the template boundary... - A single RNA-dependent RNA polymerase assembles with mutually exclusive nucleotidyl transferase subunits to direct different pathways of small RNA biogenesisSuzanne Rebecca Lee
Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720 3200, USA
RNA 15:1363-74. 2009..The biochemical and biological phenotypes of RDRC subunit disruption reveal a previously unanticipated complexity of Rdr-dependent sRNA biogenesis in vivo... - Human Y5 RNA specializes a Ro ribonucleoprotein for 5S ribosomal RNA quality controlJ Robert Hogg
Department of Molecular and Cell Biology, University of California at Berkely, Berkeley, California 94720, USA
Genes Dev 21:3067-72. 2007..These findings reveal a contribution of Y5 to 5S surveillance and suggest that interactions between Ro-Y5 and L5-5S RNPs establish 5S RNA as a target of quality control... - Physical and functional coupling of RNA-dependent RNA polymerase and Dicer in the biogenesis of endogenous siRNAsSuzanne R Lee
Department of Molecular and Cell Biology, University of California Berkeley, 142 Life Sciences Addition 3200, Berkeley, California 94720 3200, USA
Nat Struct Mol Biol 14:604-10. 2007..These findings suggest that sRNA strand bias arises from Dcr2 processing polarity, conferred by physical and functional coupling of RDRC and Dicer enzymes... - Two classes of endogenous small RNAs in Tetrahymena thermophilaSuzanne R Lee
Department of Molecular and Cell Biology, University of California at Berkeley, 94720-3204, USA
Genes Dev 20:28-33. 2006..These findings reveal the existence of distinct endogenous RNAi pathways in the unicellular T. thermophila, a complexity previously demonstrated only in multicellular organisms... - The telomerase reverse transcriptase regulates chromatin state and DNA damage responsesKenkichi Masutomi
Department of Medical Oncology and Biostatistical Science, Dana Farber Cancer Institute, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 102:8222-7. 2005..Cells lacking hTERT exhibit increased radiosensitivity, diminished capacity for DNA repair, and fragmented chromosomes, demonstrating that loss of hTERT impairs the DNA damage response... - Structural study of elements of Tetrahymena telomerase RNA stem-loop IV domain important for functionRebecca J Richards
Department of Molecular Biology and Biochemistry, University of South Bohemia, Czech Republic
RNA 12:1475-85. 2006..The results suggest that during holoenzyme assembly the protein p65 recognizes a bend in stem IV, and this binding to central stem IV helps to position the structured loop IV for interaction with TERT and other region(s) of TER... - Stepwise protein-mediated RNA folding directs assembly of telomerase ribonucleoproteinMichael D Stone
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA
Nature 446:458-61. 2007..These results identify the RNA folding pathway during telomerase biogenesis and define the mechanism of action for an essential telomerase holoenzyme protein... - Macronuclear genome sequence of the ciliate Tetrahymena thermophila, a model eukaryoteJonathan A Eisen
The Institute for Genomic Research, Rockville, Maryland, United States of America
PLoS Biol 4:e286. 2006..thermophila an ideal model for functional genomic studies to address biological, biomedical, and biotechnological questions of fundamental importance... - High-resolution physical and functional mapping of the template adjacent DNA binding site in catalytically active telomeraseErez Romi
Department of Biology, Technion Israel Institute of Technology, Haifa 32000, Israel
Proc Natl Acad Sci U S A 104:8791-6. 2007..Our data also suggest a movement of the TERT active site relative to the template-adjacent single-stranded DNA binding site within a cycle of repeat synthesis...