Research Topics
Species | James CollawnSummaryAffiliation: University of Alabama at Birmingham Country: USA Publications
Research Grants
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Publications
The CFTR and ENaC debate: how important is ENaC in CF lung disease?James F Collawn
Department of Cell, Developmental and Integrative Biology, School of Medicine, University of Alabama at Birmingham, 35294 0005, USA
Am J Physiol Lung Cell Mol Physiol 302:L1141-6. 2012..Here we discuss the arguments for and against the role of the epithelial sodium channel, ENaC, in the development of CF lung disease...- Targets for cystic fibrosis therapy: proteomic analysis and correction of mutant cystic fibrosis transmembrane conductance regulatorJames F Collawn
University of Alabama at Birmingham, Department of Cell Biology, Birmingham, AL, USA
Expert Rev Proteomics 7:495-506. 2010.... 
Endoplasmic reticulum stress and the unfolded protein response regulate genomic cystic fibrosis transmembrane conductance regulator expressionAndras Rab
Dept of Cell Biology, Univ of Alabama at Birmingham, 1918 Univ Blvd, MCLM 760, Birmingham, AL 35294 0005, USA
Am J Physiol Cell Physiol 292:C756-66. 2007....
CFTR expression regulation by the unfolded protein responseRafal Bartoszewski
Department of Cell Biology, University of Alabama at Birmingham, Birmingham, Alabama, USA
Methods Enzymol 491:3-24. 2011..Here, we provide a detailed description of the methods we employ to study CFTR expression regulation by the UPR. Similar approaches are applicable in studies on other genes and how they are affected by the UPR...- Activation of the unfolded protein response by deltaF508 CFTRRafal Bartoszewski
Department of Cell Biology, University of Alabama at Birmingham, Birmingham, AL 35294 0005, USA
Am J Respir Cell Mol Biol 39:448-57. 2008.... - Efficient intracellular processing of the endogenous cystic fibrosis transmembrane conductance regulator in epithelial cell linesKaroly Varga
Department of Cell Biology, Genetics and Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
J Biol Chem 279:22578-84. 2004..Our results question the structural instability model of wild type CFTR and indicate that epithelial cells endogenously expressing CFTR efficiently process this protein to post-Golgi compartments... - A synonymous single nucleotide polymorphism in DeltaF508 CFTR alters the secondary structure of the mRNA and the expression of the mutant proteinRafal A Bartoszewski
Department of Cell Biology, University of Alabama at Birmingham, Birmingham, Alabama 35294 0005, USA
J Biol Chem 285:28741-8. 2010..Our studies suggest that in addition to modifier genes, SNPs may also contribute to the differences observed in the symptoms of various DeltaF508 homozygous CF patients... - VCP/p97 AAA-ATPase does not interact with the endogenous wild-type cystic fibrosis transmembrane conductance regulatorRebecca F Goldstein
Department of Cell Biology, Comprehensive Cancer Center, and Gregory Fleming James Cystic Fibrosis Center, University of Alabama at Birmingham, Birmingham, Alabama 35294 0005, USA
Am J Respir Cell Mol Biol 36:706-14. 2007.... - Ablation of internalization signals in the carboxyl-terminal tail of the cystic fibrosis transmembrane conductance regulator enhances cell surface expressionKrisztina Peter
Department of Cell Biology, Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama at Birmingham, 1918 University Boulevard, Birmingham, AL 35294-0005, USA
J Biol Chem 277:49952-7. 2002..This signal regulates CFTR surface expression but not CFTR biogenesis, degradation, or chloride channel function... - Several PATCHED1 missense mutations display activity in patched1-deficient fibroblastsEvans C Bailey
Department of Cell Biology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
J Biol Chem 277:33632-40. 2002..These studies suggest that some tumors and disease phenotypes may arise from small reductions in PTCH1 activity... - Functional stability of rescued delta F508 cystic fibrosis transmembrane conductance regulator in airway epithelial cellsAsta Jurkuvenaite
Department of Cell Biology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
Am J Respir Cell Mol Biol 42:363-72. 2010.... - DETANO and nitrated lipids increase chloride secretion across lung airway cellsLan Chen
Department of Anesthesiology, University of Alabama at Birmingham, Birmingham, AL 35205 3703, USA
Am J Respir Cell Mol Biol 39:150-62. 2008..These findings suggest that small concentrations of NO donors may prove beneficial in stimulating Cl- secretion across airway cells without promoting alveolar edema... - Enhanced cell-surface stability of rescued DeltaF508 cystic fibrosis transmembrane conductance regulator (CFTR) by pharmacological chaperonesKaroly Varga
Department of Cell Biology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
Biochem J 410:555-64. 2008..The results indicate that small molecular correctors may reproduce the effect of incubation at the permissive temperature, not only by rescuing DeltaF508 CFTR from ERAD, but also by enhancing its cell-surface stability... - Reactive oxygen nitrogen species decrease cystic fibrosis transmembrane conductance regulator expression and cAMP-mediated Cl- secretion in airway epitheliaZsuzsa Bebok
Department of Medicine, and The Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama, Birmingham, Alabama 35233, USA
J Biol Chem 277:43041-9. 2002..Decreased levels and function of normal CFTR may account for some of the cystic fibrosis-like symptoms that occur in chronic inflammatory lung diseases associated with increased NO production... - Mutations in the amino terminus of the cystic fibrosis transmembrane conductance regulator enhance endocytosisAsta Jurkuvenaite
Department of Cell Biology, University of Alabama at Birmingham, AL 35294, USA
J Biol Chem 281:3329-34. 2006..These two additive effects contribute to the loss of surface expression and the associated defect in chloride conductance that is consistent with a disease phenotype... - Failure of cAMP agonists to activate rescued deltaF508 CFTR in CFBE41o- airway epithelial monolayersZsuzsa Bebok
Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama at Birmingham, Birmingham, AL 35233, USA
J Physiol 569:601-15. 2005.... - Plasma membrane CFTR regulates RANTES expression via its C-terminal PDZ-interacting motifKim Estell
Department of Physiology and Biophysics The Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
Mol Cell Biol 23:594-606. 2003..Together, these data suggest that CFTR modulates gene expression in airway epithelial cells while located in a macromolecular signaling complex at the plasma membrane... - Transient transfection of polarized epithelial monolayers with CFTR and reporter genes using efficacious lipidsTorry A Tucker
Department of Physiology and Biophysics, University of Alabama at Birmingham, 35294, USA
Am J Physiol Cell Physiol 284:C791-804. 2003..Transient transfection of epithelial monolayers provides a powerful system in which to express the cDNA of any epithelium-specific protein transiently in a native polarized epithelium to study protein function... - AP2 adaptor complex-dependent internalization of CD5: differential regulation in T and B cellsXianghuai Lu
Division of Clinical Immunology and Rheumatology, Departments of Medicine, Cell Biology, and Microbiology, University of Alabama, Birmingham, AL 35294
J Immunol 168:5612-20. 2002..The ability to differentially alter posttranslational CD5 expression in T and B cells is likely to be key in regulation of Ag receptor signaling and generation of tolerance in T and B lymphocytes...
Research Grants
- Cell Biology of CFTR in Polarized EpitheliaJames Collawn; Fiscal Year: 2003....
- Cell Biology of CFTR in Polarized EpitheliaJames Collawn; Fiscal Year: 2006..abstract_text> ..
- Cell Biology of CFTR in Polarized EpitheliaJames Collawn; Fiscal Year: 2007..abstract_text> ..
- Cell Biology of CFTR in Polarized EpitheliaJames Collawn; Fiscal Year: 2009..In the proposed studies, we are examining the cell surface stability of this chloride channel and the cellular mechanisms that regulate its cell surface stability. ..
- Cell Biology of CFTR in Polarized EpitheliaJames F Collawn; Fiscal Year: 2010..In the proposed studies, we are examining the cell surface stability of this chloride channel and the cellular mechanisms that regulate its cell surface stability. ..