Alexander Cole

Summary

Affiliation: University of Central Florida
Country: USA

Publications

  1. ncbi request reprint Mechanisms and modifications of naturally occurring host defense peptides for anti-HIV microbicide development
    Colleen R Eade
    Department of Molecular Biology and Microbiology, Burnett School of Biomedical Sciences, University of Central Florida College of Medicine, Orlando, FL 32816, USA
    Curr HIV Res 10:61-72. 2012
  2. pmc Genome sequencing and analysis reveals possible determinants of Staphylococcus aureus nasal carriage
    Karthikeyan Sivaraman
    Department of Molecular Biology and Microbiology, Biomolecular Science Center, Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, USA
    BMC Genomics 9:433. 2008
  3. pmc Preformulation and stability in biological fluids of the retrocyclin RC-101, a potential anti-HIV topical microbicide
    Alexandra B Sassi
    Magee Womens Research Institute, 204 Craft Avenue, Pittsburgh, PA, 15213, USA
    AIDS Res Ther 8:27. 2011
  4. ncbi request reprint Decreased clearance of Pseudomonas aeruginosa from airways of mice deficient in lysozyme M
    Alexander M Cole
    Department of Molecular Biology and Microbiology, Biomolecular Science Center, University of Central Florida, Orlando 32816, USA
    J Leukoc Biol 78:1081-5. 2005
  5. ncbi request reprint HIV-1 adapts to a retrocyclin with cationic amino acid substitutions that reduce fusion efficiency of gp41
    Amy L Cole
    Department of Molecular Biology and Microbiology, Burnett College of Biomedical Sciences, University of Central Florida, FL 32816, USA
    J Immunol 176:6900-5. 2006
  6. ncbi request reprint Innate host defense of human vaginal and cervical mucosae
    A M Cole
    Department of Molecular Biology and Microbiology, Biomolecular Science Center, University of Central Florida, Orlando 32816 2364, USA
    Curr Top Microbiol Immunol 306:199-230. 2006
  7. pmc The retrocyclin analogue RC-101 prevents human immunodeficiency virus type 1 infection of a model human cervicovaginal tissue construct
    Amy L Cole
    Department of Molecular Biology and Microbiology, Biomolecular Science Center, Burnett College of Biomedical Sciences at University of Central Florida, Orlando, FL 32816, USA
    Immunology 121:140-5. 2007
  8. ncbi request reprint Minidefensins and other antimicrobial peptides: candidate anti-HIV microbicides
    Alexander M Cole
    UCLA School of Medicine, Department of Medicine, Division of Pulmonary and Critical Care Medicine, Los Angeles, CA 90095, USA
    Expert Opin Ther Targets 7:329-41. 2003
  9. ncbi request reprint Antimicrobial polypeptides are key anti-HIV-1 effector molecules of cervicovaginal host defense
    Alexander M Cole
    Department of Molecular Biology and Microbiology, University of Central Florida, Orlando, FL, USA
    Am J Reprod Immunol 59:27-34. 2008
  10. pmc The formulated microbicide RC-101 was safe and antivirally active following intravaginal application in pigtailed macaques
    Alexander M Cole
    Department of Molecular Biology and Microbiology, Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, Florida, USA
    PLoS ONE 5:e15111. 2010

Research Grants

Collaborators

Detail Information

Publications37

  1. ncbi request reprint Mechanisms and modifications of naturally occurring host defense peptides for anti-HIV microbicide development
    Colleen R Eade
    Department of Molecular Biology and Microbiology, Burnett School of Biomedical Sciences, University of Central Florida College of Medicine, Orlando, FL 32816, USA
    Curr HIV Res 10:61-72. 2012
    ....
  2. pmc Genome sequencing and analysis reveals possible determinants of Staphylococcus aureus nasal carriage
    Karthikeyan Sivaraman
    Department of Molecular Biology and Microbiology, Biomolecular Science Center, Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, USA
    BMC Genomics 9:433. 2008
    ..Herein, we have set a genomic foundation for unraveling the bacterial determinants of nasal carriage in S. aureus...
  3. pmc Preformulation and stability in biological fluids of the retrocyclin RC-101, a potential anti-HIV topical microbicide
    Alexandra B Sassi
    Magee Womens Research Institute, 204 Craft Avenue, Pittsburgh, PA, 15213, USA
    AIDS Res Ther 8:27. 2011
    ..abstract:..
  4. ncbi request reprint Decreased clearance of Pseudomonas aeruginosa from airways of mice deficient in lysozyme M
    Alexander M Cole
    Department of Molecular Biology and Microbiology, Biomolecular Science Center, University of Central Florida, Orlando 32816, USA
    J Leukoc Biol 78:1081-5. 2005
    ..aeruginosa compared with their lys M+/- or lys M+/+ littermates. Lysozyme contributes to optimal clearance of P. aeruginosa from the murine airways...
  5. ncbi request reprint HIV-1 adapts to a retrocyclin with cationic amino acid substitutions that reduce fusion efficiency of gp41
    Amy L Cole
    Department of Molecular Biology and Microbiology, Burnett College of Biomedical Sciences, University of Central Florida, FL 32816, USA
    J Immunol 176:6900-5. 2006
    ..These findings also suggest that interactions between theta-defensins and gp41 may contribute to the ability of these cyclic minidefensins to prevent HIV-1 entry into target cells...
  6. ncbi request reprint Innate host defense of human vaginal and cervical mucosae
    A M Cole
    Department of Molecular Biology and Microbiology, Biomolecular Science Center, University of Central Florida, Orlando 32816 2364, USA
    Curr Top Microbiol Immunol 306:199-230. 2006
    ..On the contrary, the abundance and multiplicity of antimicrobial peptides and proteins suggest protection of the cervicovaginal mucosa may be best realized from the aggregate effector molecules...
  7. pmc The retrocyclin analogue RC-101 prevents human immunodeficiency virus type 1 infection of a model human cervicovaginal tissue construct
    Amy L Cole
    Department of Molecular Biology and Microbiology, Biomolecular Science Center, Burnett College of Biomedical Sciences at University of Central Florida, Orlando, FL 32816, USA
    Immunology 121:140-5. 2007
    ..Collectively, these data support the potential development of RC-101 as a topical microbicide to prevent HIV-1 infection and transmission...
  8. ncbi request reprint Minidefensins and other antimicrobial peptides: candidate anti-HIV microbicides
    Alexander M Cole
    UCLA School of Medicine, Department of Medicine, Division of Pulmonary and Critical Care Medicine, Los Angeles, CA 90095, USA
    Expert Opin Ther Targets 7:329-41. 2003
    ..Strategies to develop potent peptide antibiotics based on defensin and minidefensin templates are promising in the development of antiviral therapeutics and preventatives...
  9. ncbi request reprint Antimicrobial polypeptides are key anti-HIV-1 effector molecules of cervicovaginal host defense
    Alexander M Cole
    Department of Molecular Biology and Microbiology, University of Central Florida, Orlando, FL, USA
    Am J Reprod Immunol 59:27-34. 2008
    ..Emphasis is placed on the theta-defensin (retrocyclin) class of anti-HIV-1 peptides and their potential for development as topical microbicides to prevent HIV-1 transmission...
  10. pmc The formulated microbicide RC-101 was safe and antivirally active following intravaginal application in pigtailed macaques
    Alexander M Cole
    Department of Molecular Biology and Microbiology, Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, Florida, USA
    PLoS ONE 5:e15111. 2010
    ..Herein, film-formulated RC-101 was assessed for its antiviral activity in vitro, safety in vivo, retention in the cervix and vagina, and ability to remain active against HIV-1 and SHIV after intravaginal application in macaques...
  11. ncbi request reprint Antimicrobial peptide microbicides targeting HIV
    Alexander M Cole
    Department of Molecular Biology and Microbiology, Biomolecular Science Center, University of Central Florida, Bldg 20, Room 136, 4000 Central Florida Blvd, Orlando, FL 32816 2364, USA
    Protein Pept Lett 12:41-7. 2005
    ..Particular emphasis is placed on the defensin family of peptides...
  12. ncbi request reprint Retrocyclin RC-101 overcomes cationic mutations on the heptad repeat 2 region of HIV-1 gp41
    Christopher A Fuhrman
    Molecular Biology and Microbiology, Biomolecular Science Center, Burnett College of Biomedical Sciences at University of Central Florida, Orlando, FL 32816, USA
    FEBS J 274:6477-87. 2007
    ....
  13. pmc Reawakening retrocyclins: ancestral human defensins active against HIV-1
    Nitya Venkataraman
    Department of Molecular Biology and Microbiology, Burnett College of Biomedical Sciences, University of Central Florida, Orlando, Florida, United States of America
    PLoS Biol 7:e95. 2009
    ..The ability to reawaken retrocyclin genes from their 7 million years of slumber using aminoglycosides could provide a novel way to secure enhanced resistance to HIV-1 infection...
  14. pmc Retrocyclin: a primate peptide that protects cells from infection by T- and M-tropic strains of HIV-1
    Alexander M Cole
    Department of Medicine, AIDS Institute, University of California School of Medicine, Los Angeles, CA 90095, USA
    Proc Natl Acad Sci U S A 99:1813-8. 2002
    ..These findings suggest that retrocyclin interferes with an early stage of HIV-1 infection and that retrocyclin-like agents might be useful topical agents to prevent sexually acquired HIV-1 infections...
  15. ncbi request reprint Retrocyclin, an antiretroviral theta-defensin, is a lectin
    Wei Wang
    Department of Medicine, University of California, Los Angeles, CA 90095, USA
    J Immunol 170:4708-16. 2003
    ..Retrocyclin's ability to recognize and bind carbohydrate-containing surface molecules is integrally related to its ability to protect cells from HIV-1 infection...
  16. ncbi request reprint Cationic polypeptides are required for anti-HIV-1 activity of human vaginal fluid
    Nitya Venkataraman
    Department of Molecular Biology and Microbiology, Biomolecular Science Center, University of Central Florida, Orlando, FL 32816 2364, USA
    J Immunol 175:7560-7. 2005
    ..These results suggest that synergism between cationic polypeptides is complex, and full anti-HIV-1 activity probably involves the aggregate of the cationic peptides and proteins in vaginal fluid...
  17. ncbi request reprint PRO 2000 elicits a decline in genital tract immune mediators without compromising intrinsic antimicrobial activity
    Marla J Keller
    Department of Medicine, Mount Sinai School of Medicine, New York, New York 10029, USA
    AIDS 21:467-76. 2007
    ..The objective of this study was to examine the effect of 14 daily applications of 0.5% PRO 2000 or placebo gel on mediators of mucosal immunity and intrinsic antimicrobial activity...
  18. ncbi request reprint The role of defensins in lung biology and therapy
    Alexander M Cole
    Department of Medicine, Division of Pulmonary and Critical Care Medicine, UCLA School of Medicine, Los Angeles, California 90095, USA
    Am J Respir Med 1:249-59. 2002
    ..Strategies to develop potent peptide-based microbicides are promising in the struggle against increasingly resistant pathogens...
  19. ncbi request reprint Cationic polypeptides are required for antibacterial activity of human airway fluid
    Alexander M Cole
    Department of Medicine, Division of Pulmonary and Critical Care Medicine, and Will Rogers Institute Pulmonary Research Laboratory, University of California, Los Angeles, School of Medicine, Los Angeles, CA 90095, USA
    J Immunol 169:6985-91. 2002
    ..aeruginosa...
  20. ncbi request reprint Increased inflammation in lysozyme M-deficient mice in response to Micrococcus luteus and its peptidoglycan
    Tomas Ganz
    Department of Medicine, David Geffen School of Medicine, Los Angeles, CA 90095 1690, USA
    Blood 101:2388-92. 2003
    ..Our data indicate that tissue injury is normally limited by prompt degradation of bacterial macromolecules that trigger innate immunity and inflammation...
  21. pmc Suppression of innate immunity by a nasal carriage strain of Staphylococcus aureus increases its colonization on nasal epithelium
    Gerry A Quinn
    Laboratory of Innate Host Defense, Department of Molecular Biology and Microbiology, Burnett College of Biomedical Sciences, University of Central Florida, Orlando, FL 32816, USA
    Immunology 122:80-9. 2007
    ..These results suggest that carrier strains of S. aureus retain a competitive advantage over non-carrier strains by delaying the host's innate response to epithelial colonization and infection...
  22. pmc Cationic polypeptides contribute to the anti-HIV-1 activity of human seminal plasma
    Julie A Martellini
    Department of Molecular Biology and Microbiology, Biomolecular Science Center, Burnett School of Biomedical Sciences at University of Central Florida, Orlando, Florida, USA
    FASEB J 23:3609-18. 2009
    ..Collectively, these results indicate that the cationic polypeptide fraction of SP is active against HIV-1, and that semenogelin-derived peptides contribute to the intrinsic anti-HIV-1 activity of SP...
  23. pmc Subversion of interleukin-1-mediated host defence by a nasal carrier strain of Staphylococcus aureus
    Gerry A Quinn
    Department of Molecular Biology and Microbiology, Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, FL 32816, USA
    Immunology 128:e222-9. 2009
    ..aureus are sensitive to IL-1, they display a significant colonization advantage by both preventing the host from expressing IL-1 and elaborating a protective biofilm...
  24. ncbi request reprint Minidefensins: antimicrobial peptides with activity against HIV-1
    Alexander M Cole
    David Geffen School of Medicine at UCLA, Department of Medicine, Division of Pulmonary and Critical Care Medicine, Los Angeles, CA 90095, USA
    Curr Pharm Des 9:1463-73. 2003
    ..In addition to describing critical elements of their structure and unusual mode of formation, we will venture some predictions about using theta-defensins as antiviral agents...
  25. ncbi request reprint Antimicrobial peptides and proteins in the CF airway
    Alexander M Cole
    Department of Medicine, Will Rogers Institute Pulmonary Research Laboratory, UCLA School of Medicine, Los Angeles, CA, USA
    Methods Mol Med 70:447-64. 2002
  26. ncbi request reprint Activity of alpha- and theta-defensins against primary isolates of HIV-1
    Wei Wang
    Department of Medicine, David Geffen School of Medicine at University of California Los Angeles, 10833 LeConte Avenue, Los Angeles, CA 90095, USA
    J Immunol 173:515-20. 2004
    ....
  27. ncbi request reprint Antibacterial activity of peptides derived from envelope glycoproteins of HIV-1
    Alexander M Cole
    Department of Medicine, Division of Pulmonary and Critical Care Medicine, UCLA School of Medicine, 10833 Le Conte Ave, Room CHS 37 055, Los Angeles, CA 90095 1690, USA
    FEBS Lett 535:195-9. 2003
    ..The strategies employed herein could uncover additional antimicrobial peptides from envelope proteins of other lytic viruses...
  28. pmc Pathogenesis gene families in the common minimal genome of Staphylococcus aureus are hypervariable
    Karthikeyan Sivaraman
    Department of Molecular Biology and Microbiology, Biomolecular Science Center, Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, 4000 Central Florida Blvd, Orlando, FL 32816, USA
    FEBS Lett 583:1304-8. 2009
    ..Variation in key pathogenesis genes in S. aureus might predispose them to functional modulation, thereby playing an important role in evasion of host immunity...
  29. pmc Codon choice in genes depends on flanking sequence information--implications for theoretical reverse translation
    Karthikeyan Sivaraman
    Department of Molecular Biology and Microbiology, Burnett School of Biomedical Sciences, University of Central Florida, Orlando, FL, 32816, USA
    Nucleic Acids Res 36:e16. 2008
    ..Furthermore, we revealed a significant increase in coverage, at 80% identity, from < 20% (CSM) to > 75% (c-NCM). The algorithms, their applications and implications are discussed herein...
  30. ncbi request reprint Retrocyclins: using past as prologue
    Alexander M Cole
    Department of Molecular Biology, Biomolecular Science Center, University of Central Florida, Orlando, FL 32816 2364, USA
    Curr Protein Pept Sci 5:373-81. 2004
    ..This unusual combination makes Theta-defensins intriguing molecular prototypes that could be used to design novel carbohydrate-binding or antiviral agents...
  31. ncbi request reprint Lysozyme levels in the nasal secretions of patients with perennial allergic rhinitis and recurrent sinusitis
    V Cuneyt Kalfa
    Division of Pediatric Immunology, Allergy, Rheumatology, Department of Pediatrics, University of California at Los Angeles, Los Angeles, California, USA
    Ann Allergy Asthma Immunol 93:288-92. 2004
    ..Anatomic abnormalities at the osteomeatal complex or ciliary dysfunction may play a significant role in some patients. However, for most patients with allergy, the determinants of RS are unknown...
  32. ncbi request reprint Increased bronchoalveolar lavage human beta-defensin type 2 in bronchiolitis obliterans syndrome after lung transplantation
    David J Ross
    Division of Pulmonary and Critical Care Medicine and the Will Rogers Institute for Pulmonary Research, David Geffen School of Medicine U C L A Medical Center, Los Angeles, CA 90095 1690, USA
    Transplantation 78:1222-4. 2004
    ..We therefore speculate that complex interactions between adaptive and innate immunity may contribute to the propagation of airway inflammation in BOS...
  33. ncbi request reprint The theta-defensin, retrocyclin, inhibits HIV-1 entry
    Carsten Munk
    Infectious Disease Laboratory, Salk Institute for Biological Studies, San Diego, CA 92037, USA
    AIDS Res Hum Retroviruses 19:875-81. 2003
    ..In the aggregate, these results suggest that retrocyclin blocks an entry step in HIV-1 replication. Retrocyclin represents a new class of small molecule HIV-1 entry inhibitors...
  34. ncbi request reprint RC-101, a retrocyclin-1 analogue with enhanced activity against primary HIV type 1 isolates
    Sherry M Owen
    HIV Immunology and Diagnostic Branch, Division of AIDS, STD, and TB Laboratory Research, National Center for HIV, STD and TB Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    AIDS Res Hum Retroviruses 20:1157-65. 2004
    ..The performance of RC-101 suggests that additional "engineering" could further enhance the antiviral properties of theta-defensins...
  35. ncbi request reprint Theta-defensins prevent HIV-1 Env-mediated fusion by binding gp41 and blocking 6-helix bundle formation
    Stephen A Gallo
    Center for Cancer Research Nanobiology Program, NCI Frederick, National Institutes of Health, Frederick, Maryland 21702, USA
    J Biol Chem 281:18787-92. 2006
    ..This mode of action, although novel for an innate effector molecule, resembles the mechanism of peptidic entry inhibitors based on portions of the gp41 sequence...
  36. ncbi request reprint theta-Defensin pseudogenes in HIV-1-exposed, persistently seronegative female sex-workers from Thailand
    Chunfu Yang
    Division of AIDS, STD, and TB Laboratory Research, National Center for HIV, STD, and TB Prevention, Centers for Disease Control and Prevention CDC, Mail Stop D 12, 1600 Clifton Road, Atlanta, GA 30333, USA
    Infect Genet Evol 5:11-5. 2005
    ....
  37. doi request reprint Antimicrobial peptides in innate immune responses
    Ole E Sørensen
    Division of Infection Medicine, Department of Clinical Sciences, Lund University, Lund, Sweden
    Contrib Microbiol 15:61-77. 2008
    ..AMPs are attractive candidates for development of novel antibiotics due to their in vivo activity profile and some peptides may serve as templates for further drug development...

Research Grants17

  1. Retrocyclins: Circular Defensins Active Against HIV-1
    Alexander M Cole; Fiscal Year: 2010
    ..Availability of such agents, in the form of a cream or gel, would empower vulnerable sexual partners by providing them with an invisible, effective means of protection. ..
  2. Nasal carriage of S. aureus: host-pathogen interactions
    Alexander M Cole; Fiscal Year: 2010
    ..abstract_text> ..
  3. Nasal carriage of S. aureus: host-pathogen interactions
    Alexander Cole; Fiscal Year: 2007
    ..Our studies will characterize factors responsible for SA carriage, and will continue to develop a very useful and natural model for studying the interactions of bacteria with a readily accessible mucosal surface in humans. ..
  4. Retrocyclins: Circular Defensins Active Against HIV-1
    Alexander Cole; Fiscal Year: 2005
    ..Availability of such agents, in the form of retrocyclin-containing creams or gels, would empower vulnerable sexual partners by providing them with an invisible, effective means of protection. ..
  5. Proteomics of Staphylococcus aureus nasal carriage
    Alexander Cole; Fiscal Year: 2003
    ....