Research Topics
Species | Alexander ColeSummaryAffiliation: University of Central Florida Country: USA Publications
Research Grants
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Publications
Mechanisms and modifications of naturally occurring host defense peptides for anti-HIV microbicide developmentColleen R Eade
Department of Molecular Biology and Microbiology, Burnett School of Biomedical Sciences, University of Central Florida College of Medicine, Orlando, FL 32816, USA
Curr HIV Res 10:61-72. 2012....
Genome sequencing and analysis reveals possible determinants of Staphylococcus aureus nasal carriageKarthikeyan Sivaraman
Department of Molecular Biology and Microbiology, Biomolecular Science Center, Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, USA
BMC Genomics 9:433. 2008..Herein, we have set a genomic foundation for unraveling the bacterial determinants of nasal carriage in S. aureus...- Preformulation and stability in biological fluids of the retrocyclin RC-101, a potential anti-HIV topical microbicideAlexandra B Sassi
Magee Womens Research Institute, 204 Craft Avenue, Pittsburgh, PA, 15213, USA
AIDS Res Ther 8:27. 2011..abstract:.. - Decreased clearance of Pseudomonas aeruginosa from airways of mice deficient in lysozyme MAlexander M Cole
Department of Molecular Biology and Microbiology, Biomolecular Science Center, University of Central Florida, Orlando 32816, USA
J Leukoc Biol 78:1081-5. 2005..aeruginosa compared with their lys M+/- or lys M+/+ littermates. Lysozyme contributes to optimal clearance of P. aeruginosa from the murine airways... - HIV-1 adapts to a retrocyclin with cationic amino acid substitutions that reduce fusion efficiency of gp41Amy L Cole
Department of Molecular Biology and Microbiology, Burnett College of Biomedical Sciences, University of Central Florida, FL 32816, USA
J Immunol 176:6900-5. 2006..These findings also suggest that interactions between theta-defensins and gp41 may contribute to the ability of these cyclic minidefensins to prevent HIV-1 entry into target cells... - Innate host defense of human vaginal and cervical mucosaeA M Cole
Department of Molecular Biology and Microbiology, Biomolecular Science Center, University of Central Florida, Orlando 32816 2364, USA
Curr Top Microbiol Immunol 306:199-230. 2006..On the contrary, the abundance and multiplicity of antimicrobial peptides and proteins suggest protection of the cervicovaginal mucosa may be best realized from the aggregate effector molecules... - The retrocyclin analogue RC-101 prevents human immunodeficiency virus type 1 infection of a model human cervicovaginal tissue constructAmy L Cole
Department of Molecular Biology and Microbiology, Biomolecular Science Center, Burnett College of Biomedical Sciences at University of Central Florida, Orlando, FL 32816, USA
Immunology 121:140-5. 2007..Collectively, these data support the potential development of RC-101 as a topical microbicide to prevent HIV-1 infection and transmission... - Minidefensins and other antimicrobial peptides: candidate anti-HIV microbicidesAlexander M Cole
UCLA School of Medicine, Department of Medicine, Division of Pulmonary and Critical Care Medicine, Los Angeles, CA 90095, USA
Expert Opin Ther Targets 7:329-41. 2003..Strategies to develop potent peptide antibiotics based on defensin and minidefensin templates are promising in the development of antiviral therapeutics and preventatives... - Antimicrobial polypeptides are key anti-HIV-1 effector molecules of cervicovaginal host defenseAlexander M Cole
Department of Molecular Biology and Microbiology, University of Central Florida, Orlando, FL, USA
Am J Reprod Immunol 59:27-34. 2008..Emphasis is placed on the theta-defensin (retrocyclin) class of anti-HIV-1 peptides and their potential for development as topical microbicides to prevent HIV-1 transmission... - The formulated microbicide RC-101 was safe and antivirally active following intravaginal application in pigtailed macaquesAlexander M Cole
Department of Molecular Biology and Microbiology, Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, Florida, USA
PLoS ONE 5:e15111. 2010..Herein, film-formulated RC-101 was assessed for its antiviral activity in vitro, safety in vivo, retention in the cervix and vagina, and ability to remain active against HIV-1 and SHIV after intravaginal application in macaques... - Antimicrobial peptide microbicides targeting HIVAlexander M Cole
Department of Molecular Biology and Microbiology, Biomolecular Science Center, University of Central Florida, Bldg 20, Room 136, 4000 Central Florida Blvd, Orlando, FL 32816 2364, USA
Protein Pept Lett 12:41-7. 2005..Particular emphasis is placed on the defensin family of peptides... - Retrocyclin RC-101 overcomes cationic mutations on the heptad repeat 2 region of HIV-1 gp41Christopher A Fuhrman
Molecular Biology and Microbiology, Biomolecular Science Center, Burnett College of Biomedical Sciences at University of Central Florida, Orlando, FL 32816, USA
FEBS J 274:6477-87. 2007.... - Reawakening retrocyclins: ancestral human defensins active against HIV-1Nitya Venkataraman
Department of Molecular Biology and Microbiology, Burnett College of Biomedical Sciences, University of Central Florida, Orlando, Florida, United States of America
PLoS Biol 7:e95. 2009..The ability to reawaken retrocyclin genes from their 7 million years of slumber using aminoglycosides could provide a novel way to secure enhanced resistance to HIV-1 infection... - Retrocyclin: a primate peptide that protects cells from infection by T- and M-tropic strains of HIV-1Alexander M Cole
Department of Medicine, AIDS Institute, University of California School of Medicine, Los Angeles, CA 90095, USA
Proc Natl Acad Sci U S A 99:1813-8. 2002..These findings suggest that retrocyclin interferes with an early stage of HIV-1 infection and that retrocyclin-like agents might be useful topical agents to prevent sexually acquired HIV-1 infections... - Retrocyclin, an antiretroviral theta-defensin, is a lectinWei Wang
Department of Medicine, University of California, Los Angeles, CA 90095, USA
J Immunol 170:4708-16. 2003..Retrocyclin's ability to recognize and bind carbohydrate-containing surface molecules is integrally related to its ability to protect cells from HIV-1 infection... - Cationic polypeptides are required for anti-HIV-1 activity of human vaginal fluidNitya Venkataraman
Department of Molecular Biology and Microbiology, Biomolecular Science Center, University of Central Florida, Orlando, FL 32816-2364, USA
J Immunol 175:7560-7. 2005..These results suggest that synergism between cationic polypeptides is complex, and full anti-HIV-1 activity probably involves the aggregate of the cationic peptides and proteins in vaginal fluid... - PRO 2000 elicits a decline in genital tract immune mediators without compromising intrinsic antimicrobial activityMarla J Keller
Department of Medicine, Mount Sinai School of Medicine, New York, New York 10029, USA
AIDS 21:467-76. 2007..The objective of this study was to examine the effect of 14 daily applications of 0.5% PRO 2000 or placebo gel on mediators of mucosal immunity and intrinsic antimicrobial activity... - The role of defensins in lung biology and therapyAlexander M Cole
Department of Medicine, Division of Pulmonary and Critical Care Medicine, UCLA School of Medicine, Los Angeles, California 90095, USA
Am J Respir Med 1:249-59. 2002..Strategies to develop potent peptide-based microbicides are promising in the struggle against increasingly resistant pathogens... - Cationic polypeptides are required for antibacterial activity of human airway fluidAlexander M Cole
Department of Medicine, Division of Pulmonary and Critical Care Medicine, and Will Rogers Institute Pulmonary Research Laboratory, University of California, Los Angeles, School of Medicine, Los Angeles, CA 90095, USA
J Immunol 169:6985-91. 2002..aeruginosa... - Increased inflammation in lysozyme M-deficient mice in response to Micrococcus luteus and its peptidoglycanTomas Ganz
Department of Medicine, David Geffen School of Medicine, Los Angeles, CA 90095 1690, USA
Blood 101:2388-92. 2003..Our data indicate that tissue injury is normally limited by prompt degradation of bacterial macromolecules that trigger innate immunity and inflammation... - Suppression of innate immunity by a nasal carriage strain of Staphylococcus aureus increases its colonization on nasal epitheliumGerry A Quinn
Laboratory of Innate Host Defense, Department of Molecular Biology and Microbiology, Burnett College of Biomedical Sciences, University of Central Florida, Orlando, FL 32816, USA
Immunology 122:80-9. 2007..These results suggest that carrier strains of S. aureus retain a competitive advantage over non-carrier strains by delaying the host's innate response to epithelial colonization and infection... - Cationic polypeptides contribute to the anti-HIV-1 activity of human seminal plasmaJulie A Martellini
Department of Molecular Biology and Microbiology, Biomolecular Science Center, Burnett School of Biomedical Sciences at University of Central Florida, Orlando, Florida, USA
FASEB J 23:3609-18. 2009..Collectively, these results indicate that the cationic polypeptide fraction of SP is active against HIV-1, and that semenogelin-derived peptides contribute to the intrinsic anti-HIV-1 activity of SP... - Subversion of interleukin-1-mediated host defence by a nasal carrier strain of Staphylococcus aureusGerry A Quinn
Department of Molecular Biology and Microbiology, Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, FL 32816, USA
Immunology 128:e222-9. 2009..aureus are sensitive to IL-1, they display a significant colonization advantage by both preventing the host from expressing IL-1 and elaborating a protective biofilm... - Minidefensins: antimicrobial peptides with activity against HIV-1Alexander M Cole
David Geffen School of Medicine at UCLA, Department of Medicine, Division of Pulmonary and Critical Care Medicine, Los Angeles, CA 90095, USA
Curr Pharm Des 9:1463-73. 2003..In addition to describing critical elements of their structure and unusual mode of formation, we will venture some predictions about using theta-defensins as antiviral agents... - Antimicrobial peptides and proteins in the CF airwayAlexander M Cole
Department of Medicine, Will Rogers Institute Pulmonary Research Laboratory, UCLA School of Medicine, Los Angeles, CA, USA
Methods Mol Med 70:447-64. 2002 - Activity of alpha- and theta-defensins against primary isolates of HIV-1Wei Wang
Department of Medicine, David Geffen School of Medicine at University of California-Los Angeles, 10833 LeConte Avenue, Los Angeles, CA 90095, USA
J Immunol 173:515-20. 2004.... - Antibacterial activity of peptides derived from envelope glycoproteins of HIV-1Alexander M Cole
Department of Medicine, Division of Pulmonary and Critical Care Medicine, UCLA School of Medicine, 10833 Le Conte Ave, Room CHS 37 055, Los Angeles, CA 90095 1690, USA
FEBS Lett 535:195-9. 2003..The strategies employed herein could uncover additional antimicrobial peptides from envelope proteins of other lytic viruses... - Pathogenesis gene families in the common minimal genome of Staphylococcus aureus are hypervariableKarthikeyan Sivaraman
Department of Molecular Biology and Microbiology, Biomolecular Science Center, Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, 4000 Central Florida Blvd, Orlando, FL 32816, USA
FEBS Lett 583:1304-8. 2009..Variation in key pathogenesis genes in S. aureus might predispose them to functional modulation, thereby playing an important role in evasion of host immunity... - Codon choice in genes depends on flanking sequence information--implications for theoretical reverse translationKarthikeyan Sivaraman
Department of Molecular Biology and Microbiology, Burnett School of Biomedical Sciences, University of Central Florida, Orlando, FL, 32816, USA
Nucleic Acids Res 36:e16. 2008..Furthermore, we revealed a significant increase in coverage, at 80% identity, from < 20% (CSM) to > 75% (c-NCM). The algorithms, their applications and implications are discussed herein... - Retrocyclins: using past as prologueAlexander M Cole
Department of Molecular Biology, Biomolecular Science Center, University of Central Florida, Orlando, FL 32816-2364, USA
Curr Protein Pept Sci 5:373-81. 2004..This unusual combination makes Theta-defensins intriguing molecular prototypes that could be used to design novel carbohydrate-binding or antiviral agents... - Lysozyme levels in the nasal secretions of patients with perennial allergic rhinitis and recurrent sinusitisV Cuneyt Kalfa
Division of Pediatric Immunology, Allergy, Rheumatology, Department of Pediatrics, University of California at Los Angeles, Los Angeles, California, USA
Ann Allergy Asthma Immunol 93:288-92. 2004..CONCLUSIONS: The presence of eosinophils and their products and reduced lysozyme concentrations may be critical factors that predispose the airways of patients with PAR to RS... - Increased bronchoalveolar lavage human beta-defensin type 2 in bronchiolitis obliterans syndrome after lung transplantationDavid J Ross
Division of Pulmonary and Critical Care Medicine and the Will Rogers Institute for Pulmonary Research, David Geffen School of Medicine U C L A Medical Center, Los Angeles, CA 90095 1690, USA
Transplantation 78:1222-4. 2004..We therefore speculate that complex interactions between adaptive and innate immunity may contribute to the propagation of airway inflammation in BOS... - The theta-defensin, retrocyclin, inhibits HIV-1 entryCarsten Munk
Infectious Disease Laboratory, Salk Institute for Biological Studies, San Diego, CA 92037, USA
AIDS Res Hum Retroviruses 19:875-81. 2003..In the aggregate, these results suggest that retrocyclin blocks an entry step in HIV-1 replication. Retrocyclin represents a new class of small molecule HIV-1 entry inhibitors... - RC-101, a retrocyclin-1 analogue with enhanced activity against primary HIV type 1 isolatesSherry M Owen
HIV Immunology and Diagnostic Branch, Division of AIDS, STD, and TB Laboratory Research, National Center for HIV, STD and TB Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
AIDS Res Hum Retroviruses 20:1157-65. 2004..The performance of RC-101 suggests that additional "engineering" could further enhance the antiviral properties of theta-defensins... - Theta-defensins prevent HIV-1 Env-mediated fusion by binding gp41 and blocking 6-helix bundle formationStephen A Gallo
Center for Cancer Research Nanobiology Program, NCI-Frederick, National Institutes of Health, Frederick, Maryland 21702, USA
J Biol Chem 281:18787-92. 2006..This mode of action, although novel for an innate effector molecule, resembles the mechanism of peptidic entry inhibitors based on portions of the gp41 sequence... - theta-Defensin pseudogenes in HIV-1-exposed, persistently seronegative female sex-workers from ThailandChunfu Yang
Division of AIDS, STD, and TB Laboratory Research, National Center for HIV, STD, and TB Prevention, Centers for Disease Control and Prevention CDC, Mail Stop D 12, 1600 Clifton Road, Atlanta, GA 30333, USA
Infect Genet Evol 5:11-5. 2005.... 
Antimicrobial peptides in innate immune responsesOle E Sørensen
Division of Infection Medicine, Department of Clinical Sciences, Lund University, Lund, Sweden
Contrib Microbiol 15:61-77. 2008..AMPs are attractive candidates for development of novel antibiotics due to their in vivo activity profile and some peptides may serve as templates for further drug development...
Research Grants
- Retrocyclins: Circular Defensins Active Against HIV-1Alexander M Cole; Fiscal Year: 2010..Availability of such agents, in the form of a cream or gel, would empower vulnerable sexual partners by providing them with an invisible, effective means of protection. ..
- Nasal carriage of S. aureus: host-pathogen interactionsAlexander M Cole; Fiscal Year: 2010..abstract_text> ..
- Nasal carriage of S. aureus: host-pathogen interactionsAlexander Cole; Fiscal Year: 2007..Our studies will characterize factors responsible for SA carriage, and will continue to develop a very useful and natural model for studying the interactions of bacteria with a readily accessible mucosal surface in humans. ..
- Retrocyclins: Circular Defensins Active Against HIV-1Alexander Cole; Fiscal Year: 2005..Availability of such agents, in the form of retrocyclin-containing creams or gels, would empower vulnerable sexual partners by providing them with an invisible, effective means of protection. ..
- Proteomics of Staphylococcus aureus nasal carriageAlexander Cole; Fiscal Year: 2003....