Daniel L Clemens

Summary

Affiliation: University of California
Country: USA

Publications

  1. pmc Targeted intracellular delivery of antituberculosis drugs to Mycobacterium tuberculosis-infected macrophages via functionalized mesoporous silica nanoparticles
    Daniel L Clemens
    Division of Infectious Diseases, University of California, Los Angeles, California, USA
    Antimicrob Agents Chemother 56:2535-45. 2012
  2. pmc O-antigen-deficient Francisella tularensis Live Vaccine Strain mutants are ingested via an aberrant form of looping phagocytosis and show altered kinetics of intracellular trafficking in human macrophages
    Daniel L Clemens
    Division of Infectious Diseases, Department of Medicine, University of California Los Angeles School of Medicine, Center for Health Sciences, Los Angeles, California, USA
    Infect Immun 80:952-67. 2012
  3. ncbi request reprint Uptake and intracellular fate of Francisella tularensis in human macrophages
    Daniel L Clemens
    Department of Medicine, Division of Infectious Diseases, University of California, 37 121 CHS, 10833 Le Conte Avenue, Los Angeles, CA 90095, USA
    Ann N Y Acad Sci 1105:160-86. 2007
  4. pmc Francisella tularensis phagosomal escape does not require acidification of the phagosome
    Daniel L Clemens
    Division of Infectious Diseases, Department of Medicine, UCLA School of Medicine, CHS 37 121, 10833 LeConte Avenue, Los Angeles, CA 90095 1688, USA
    Infect Immun 77:1757-73. 2009
  5. pmc Francisella tularensis enters macrophages via a novel process involving pseudopod loops
    Daniel L Clemens
    Division of Infectious Diseases, Dept of Medicine, UCLA School of Medicine, CHS 37 121, 10833 LeConte Ave, Los Angeles, CA 90095 1688, USA
    Infect Immun 73:5892-902. 2005
  6. pmc Virulent and avirulent strains of Francisella tularensis prevent acidification and maturation of their phagosomes and escape into the cytoplasm in human macrophages
    Daniel L Clemens
    Division of Infectious Diseases, Department of Medicine, UCLA School of Medicine, Los Angeles, California 90095 1688, USA
    Infect Immun 72:3204-17. 2004
  7. pmc The Mycobacterium tuberculosis phagosome in human macrophages is isolated from the host cell cytoplasm
    Daniel L Clemens
    Division of Infectious Diseases, Department of Medicine, UCLA School of Medicine, Center for Health Sciences, Los Angeles, California 90095, USA
    Infect Immun 70:5800-7. 2002
  8. pmc Mycobacterium tuberculosis and Legionella pneumophila phagosomes exhibit arrested maturation despite acquisition of Rab7
    D L Clemens
    Division of Infectious Diseases, Department of Medicine, UCLA School of Medicine, Center for Health Sciences, Los Angeles, California 90095, USA
    Infect Immun 68:5154-66. 2000
  9. pmc Deviant expression of Rab5 on phagosomes containing the intracellular pathogens Mycobacterium tuberculosis and Legionella pneumophila is associated with altered phagosomal fate
    D L Clemens
    Division of Infectious Diseases, Department of Medicine, UCLA School of Medicine, Center for Health Sciences, Los Angeles, California 90095, USA
    Infect Immun 68:2671-84. 2000
  10. pmc The metabolic activity of Mycobacterium tuberculosis, assessed by use of a novel inducible GFP expression system, correlates with its capacity to inhibit phagosomal maturation and acidification in human macrophages
    Bai Yu Lee
    Division of Infectious Diseases, Department of Medicine, University of California Los Angeles, School of Medicine, Center for Health Sciences, Los Angeles, CA 90095 1688, USA
    Mol Microbiol 68:1047-60. 2008

Collaborators

  • Genhong Cheng
  • David G Brooks
  • Jeffrey I Zink
  • Jinhai Wang
  • MARCUS HORWITZ
  • Christopher C Dascher
  • Bai Yu Lee
  • Eugene J Gillespie
  • Qingmei Jia
  • Batcha Tamilselvam
  • Shandee D Dixon
  • Gang Deng
  • Kavitha Balaji
  • Michael E Jung
  • Brian T Chamberlain
  • Chi Lee C Ho
  • Heidi J Elsaesser
  • Amandeep Gargi
  • Steven R Blanke
  • Robert Damoiseaux
  • Kenneth A Bradley
  • Bryan France
  • Juan Carlos de la Torre
  • John Colicelli
  • Yao E Wang
  • Deepa Jethwaney
  • G Harth
  • Bradford W Gibson
  • Birgit Schilling
  • Richard A Bowen
  • B Y Lee

Detail Information

Publications18

  1. pmc Targeted intracellular delivery of antituberculosis drugs to Mycobacterium tuberculosis-infected macrophages via functionalized mesoporous silica nanoparticles
    Daniel L Clemens
    Division of Infectious Diseases, University of California, Los Angeles, California, USA
    Antimicrob Agents Chemother 56:2535-45. 2012
    ..These data demonstrate that MSNP provide a versatile platform that can be functionalized to optimize the loading and intracellular release of specific drugs for the treatment of tuberculosis...
  2. pmc O-antigen-deficient Francisella tularensis Live Vaccine Strain mutants are ingested via an aberrant form of looping phagocytosis and show altered kinetics of intracellular trafficking in human macrophages
    Daniel L Clemens
    Division of Infectious Diseases, Department of Medicine, University of California Los Angeles School of Medicine, Center for Health Sciences, Los Angeles, California, USA
    Infect Immun 80:952-67. 2012
    ..Thus, the O antigen plays a role in the morphology of uptake in the presence of complement and the kinetics of intracellular growth but is not essential for escape, survival, altered membrane trafficking, or intramacrophage growth...
  3. ncbi request reprint Uptake and intracellular fate of Francisella tularensis in human macrophages
    Daniel L Clemens
    Department of Medicine, Division of Infectious Diseases, University of California, 37 121 CHS, 10833 Le Conte Avenue, Los Angeles, CA 90095, USA
    Ann N Y Acad Sci 1105:160-86. 2007
    ..Further research is needed to determine the mechanisms underlying looping phagocytosis, and the maturational arrest, fibrillar coat formation, and disruption of the phagosome...
  4. pmc Francisella tularensis phagosomal escape does not require acidification of the phagosome
    Daniel L Clemens
    Division of Infectious Diseases, Department of Medicine, UCLA School of Medicine, CHS 37 121, 10833 LeConte Avenue, Los Angeles, CA 90095 1688, USA
    Infect Immun 77:1757-73. 2009
    ..tularensis subsp. tularensis strain Schu S4 and a recent clinical isolate) or by "F. tularensis subsp. novicida," indicating that F. tularensis disrupts its phagosomal membrane by a mechanism that does not require acidification...
  5. pmc Francisella tularensis enters macrophages via a novel process involving pseudopod loops
    Daniel L Clemens
    Division of Infectious Diseases, Dept of Medicine, UCLA School of Medicine, CHS 37 121, 10833 LeConte Ave, Los Angeles, CA 90095 1688, USA
    Infect Immun 73:5892-902. 2005
    ..These findings have significant implications for understanding the intracellular biology and virulence of this extremely infectious pathogen...
  6. pmc Virulent and avirulent strains of Francisella tularensis prevent acidification and maturation of their phagosomes and escape into the cytoplasm in human macrophages
    Daniel L Clemens
    Division of Infectious Diseases, Department of Medicine, UCLA School of Medicine, Los Angeles, California 90095 1688, USA
    Infect Immun 72:3204-17. 2004
    ..The capacity of F. tularensis to alter the maturation of its phagosome and to enter the cytoplasm is likely an important element of its capacity to parasitize macrophages and has major implications for vaccine development...
  7. pmc The Mycobacterium tuberculosis phagosome in human macrophages is isolated from the host cell cytoplasm
    Daniel L Clemens
    Division of Infectious Diseases, Department of Medicine, UCLA School of Medicine, Center for Health Sciences, Los Angeles, California 90095, USA
    Infect Immun 70:5800-7. 2002
    ..Our study shows that M. tuberculosis resides in an isolated phagosome that is relatively impermeable to cytoplasmic constituents...
  8. pmc Mycobacterium tuberculosis and Legionella pneumophila phagosomes exhibit arrested maturation despite acquisition of Rab7
    D L Clemens
    Division of Infectious Diseases, Department of Medicine, UCLA School of Medicine, Center for Health Sciences, Los Angeles, California 90095, USA
    Infect Immun 68:5154-66. 2000
    ..These results demonstrate that L. pneumophila and M. tuberculosis phagosomes have receptors for Rab7 and that the altered maturation of these phagosomes is not due to a failure to acquire Rab7...
  9. pmc Deviant expression of Rab5 on phagosomes containing the intracellular pathogens Mycobacterium tuberculosis and Legionella pneumophila is associated with altered phagosomal fate
    D L Clemens
    Division of Infectious Diseases, Department of Medicine, UCLA School of Medicine, Center for Health Sciences, Los Angeles, California 90095, USA
    Infect Immun 68:2671-84. 2000
    ..The persistence of functional Rab5 on the M. tuberculosis phagosomes may enable the phagosome to retard its own maturation at an early endosomal stage...
  10. pmc The metabolic activity of Mycobacterium tuberculosis, assessed by use of a novel inducible GFP expression system, correlates with its capacity to inhibit phagosomal maturation and acidification in human macrophages
    Bai Yu Lee
    Division of Infectious Diseases, Department of Medicine, University of California Los Angeles, School of Medicine, Center for Health Sciences, Los Angeles, CA 90095 1688, USA
    Mol Microbiol 68:1047-60. 2008
    ..These studies demonstrate that metabolic activity of M. tuberculosis correlates strongly with phagosomal maturation and that the inducible GFP expression system is useful for assessing metabolic activity of intracellular M. tuberculosis...
  11. pmc The Mycobacterium bovis bacille Calmette-Guerin phagosome proteome
    Bai Yu Lee
    Division of Infectious Diseases, Department of Medicine, Center for Health Sciences, University of California Los Angeles School of Medicine, Los Angeles, California 90095 1688, USA
    Mol Cell Proteomics 9:32-53. 2010
    ..Our phagosome purification procedure and initial proteomics analyses set the stage for a quantitative comparative analysis of mycobacterial and latex bead phagosome proteomes...
  12. pmc Identification, recombinant expression, immunolocalization in macrophages, and T-cell responsiveness of the major extracellular proteins of Francisella tularensis
    Bai Yu Lee
    Division of Infectious Diseases, 37 121 Center for the Health Sciences, University of California, Los Angeles, 10833 Le Conte Avenue, Los Angeles, CA 90095 1688, USA
    Infect Immun 74:4002-13. 2006
    ..Finally, we expressed the major culture filtrate proteins that are promising vaccine candidates in Escherichia coli at high levels in soluble form to facilitate study of their immunobiology and potential role in vaccines...
  13. pmc Recombinant attenuated Listeria monocytogenes vaccine expressing Francisella tularensis IglC induces protection in mice against aerosolized Type A F. tularensis
    Qingmei Jia
    Division of Infectious Diseases, Department of Medicine, 37 121 Center for Health Sciences, School of Medicine, University of California Los Angeles, 10833 Le Conte Avenue, Los Angeles, CA 90095 1688, United States
    Vaccine 27:1216-29. 2009
    ..tularensis LVS administered by the intranasal route, a route chosen to mimic airborne infection, and, most importantly, against aerosol challenge with the highly virulent Type A F. tularensis SchuS4 strain...
  14. pmc Selective inhibitor of endosomal trafficking pathways exploited by multiple toxins and viruses
    Eugene J Gillespie
    Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, CA 90095
    Proc Natl Acad Sci U S A 110:E4904-12. 2013
    ..EGA is a powerful tool for the study of membrane trafficking and represents a class of host-targeted compounds for therapeutic development to treat infectious disease. ..
  15. pmc Novel insights into the genetics, biochemistry, and immunocytochemistry of the 30-kilodalton major extracellular protein of Mycobacterium tuberculosis
    G Harth
    Division of Infectious Diseases, Department of Medicine, University of California Los Angeles School of Medicine 90095, USA
    Infect Immun 64:3038-47. 1996
    ..tuberculosis and, at the same time, raise new and fundamental questions regarding regulatory mechanisms that control coordinate expression of the proteins at a fixed ratio...
  16. pmc Glutamine synthetase of Mycobacterium tuberculosis: extracellular release and characterization of its enzymatic activity
    G Harth
    Department of Medicine, School of Medicine, University of California, Los Angeles 90024
    Proc Natl Acad Sci U S A 91:9342-6. 1994
    ..tuberculosis phagosome, which may in turn influence phagosomal pH and phagosomelysosome fusion...
  17. pmc Purification, characterization, and genetic analysis of Mycobacterium tuberculosis urease, a potentially critical determinant of host-pathogen interaction
    D L Clemens
    Department of Medicine, University of California, Los Angeles 90095, USA
    J Bacteriol 177:5644-52. 1995
    ..The nucleotide sequence of the M. tuberculosis urease gene cluster revealed open reading frames corresponding to the urease A, B, and C subunits, as well as to the urease accessory molecules F and G...
  18. ncbi request reprint Conservation of CD1 intracellular trafficking patterns between mammalian species
    Christopher C Dascher
    Division of Rheumatology, Immunology and Allergy, Department of Pathology, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    J Immunol 169:6951-8. 2002
    ..This suggests evolutionary pressure for a conserved mechanism in mammals that allows CD1 to sample lipid Ags from various subcompartments of the endocytic system...