Karl J Clark

Summary

Affiliation: University of Minnesota
Country: USA

Publications

  1. doi request reprint Sleeping Beauty-mediated correction of Fanconi anemia type C
    Kendra A Hyland
    Discovery Genomics, Inc, Minneapolis, MN, USA
    J Gene Med 13:462-9. 2011
  2. pmc Enzymatic engineering of the porcine genome with transposons and recombinases
    Karl J Clark
    Department of Animal Science, University of Minnesota, St Paul, MN, USA
    BMC Biotechnol 7:42. 2007
  3. doi request reprint Strategies for selection marker-free swine transgenesis using the Sleeping Beauty transposon system
    Daniel F Carlson
    The Center for Genome Engineering, University of Minnesota, Minneapolis, MN 55455, USA
    Transgenic Res 20:1125-37. 2011
  4. pmc Conditional gene expression in the mouse using a Sleeping Beauty gene-trap transposon
    Aron M Geurts
    Department of Genetics, Cell Biology, and Development, The Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota, Minneapolis, MN 55455, USA
    BMC Biotechnol 6:30. 2006
  5. pmc Passport, a native Tc1 transposon from flatfish, is functionally active in vertebrate cells
    Karl J Clark
    Department of Animal Science, University of Minnesota, St Paul, MN 55108, USA
    Nucleic Acids Res 37:1239-47. 2009
  6. ncbi request reprint Phenotypic correction and long-term expression of factor VIII in hemophilic mice by immunotolerization and nonviral gene transfer using the Sleeping Beauty transposon system
    John R Ohlfest
    Department of Genetics, Cell Biology, and Development, University of Minnesota Cancer Center, Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota, 6 160 Jackson Hall, 321 Church St SE, Minneapolis, MN 55455, USA
    Blood 105:2691-8. 2005
  7. pmc Development and application of bovine and porcine oligonucleotide arrays with protein-based annotation
    John R Garbe
    Department of Animal Science, University of Minnesota, St Paul, MN 55108, USA
    J Biomed Biotechnol 2010:453638. 2010
  8. pmc Mojo Hand, a TALEN design tool for genome editing applications
    Kevin L Neff
    Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, MN, USA
    BMC Bioinformatics 14:1. 2013
  9. pmc Nicotine response genetics in the zebrafish
    Andrew M Petzold
    University of Minnesota, Minneapolis, MN 55455, USA
    Proc Natl Acad Sci U S A 106:18662-7. 2009
  10. ncbi request reprint Gene transfer into genomes of human cells by the sleeping beauty transposon system
    Aron M Geurts
    Department of Genetics, Cell Biology and Development and The Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Mol Ther 8:108-17. 2003

Collaborators

Detail Information

Publications10

  1. doi request reprint Sleeping Beauty-mediated correction of Fanconi anemia type C
    Kendra A Hyland
    Discovery Genomics, Inc, Minneapolis, MN, USA
    J Gene Med 13:462-9. 2011
    ..Our goal is to develop the SB system for nonviral complementation of Fanconi anemia (FA), a rare autosomal recessive disorder accompanied by progressive bone marrow failure...
  2. pmc Enzymatic engineering of the porcine genome with transposons and recombinases
    Karl J Clark
    Department of Animal Science, University of Minnesota, St Paul, MN, USA
    BMC Biotechnol 7:42. 2007
    ..Realizing the full potential of pig genetic engineering requires translation of the complete repertoire of genetic tools currently employed in smaller model organisms to practical use in pigs...
  3. doi request reprint Strategies for selection marker-free swine transgenesis using the Sleeping Beauty transposon system
    Daniel F Carlson
    The Center for Genome Engineering, University of Minnesota, Minneapolis, MN 55455, USA
    Transgenic Res 20:1125-37. 2011
    ..Two strategies were employed to facilitate elimination of antibiotic genes from transgenic pigs, one based on Cre-recombinase and the other by segregation of independently transposed transgenes upon breeding...
  4. pmc Conditional gene expression in the mouse using a Sleeping Beauty gene-trap transposon
    Aron M Geurts
    Department of Genetics, Cell Biology, and Development, The Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota, Minneapolis, MN 55455, USA
    BMC Biotechnol 6:30. 2006
    ..We sought to generate a transposon system that would be capable of reporting the expression pattern of a mouse gene while allowing for conditional expression of a gene of interest in a tissue- or temporal-specific pattern...
  5. pmc Passport, a native Tc1 transposon from flatfish, is functionally active in vertebrate cells
    Karl J Clark
    Department of Animal Science, University of Minnesota, St Paul, MN 55108, USA
    Nucleic Acids Res 37:1239-47. 2009
    ..The Passport transposon system thus complements the available genetic tools for the manipulation of vertebrate genomes, and may provide a unique system for studying the infiltration of vertebrate genomes by Tc1 elements...
  6. ncbi request reprint Phenotypic correction and long-term expression of factor VIII in hemophilic mice by immunotolerization and nonviral gene transfer using the Sleeping Beauty transposon system
    John R Ohlfest
    Department of Genetics, Cell Biology, and Development, University of Minnesota Cancer Center, Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota, 6 160 Jackson Hall, 321 Church St SE, Minneapolis, MN 55455, USA
    Blood 105:2691-8. 2005
    ..Thus, SB provides a nonviral means for sustained FVIII gene delivery in a mouse model of hemophilia A if the immune response is prevented...
  7. pmc Development and application of bovine and porcine oligonucleotide arrays with protein-based annotation
    John R Garbe
    Department of Animal Science, University of Minnesota, St Paul, MN 55108, USA
    J Biomed Biotechnol 2010:453638. 2010
    ..The porcine array contains 19,980 probes targeting 15,204 genes, about 76% of the genes in the Ensembl annotation of the pig genome. An initial experiment using the bovine array demonstrates the specificity and sensitivity of the array...
  8. pmc Mojo Hand, a TALEN design tool for genome editing applications
    Kevin L Neff
    Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, MN, USA
    BMC Bioinformatics 14:1. 2013
    ....
  9. pmc Nicotine response genetics in the zebrafish
    Andrew M Petzold
    University of Minnesota, Minneapolis, MN 55455, USA
    Proc Natl Acad Sci U S A 106:18662-7. 2009
    ....
  10. ncbi request reprint Gene transfer into genomes of human cells by the sleeping beauty transposon system
    Aron M Geurts
    Department of Genetics, Cell Biology and Development and The Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Mol Ther 8:108-17. 2003
    ..With the recently described improvements to the transposon itself, the SB system appears to be a potential gene-transfer tool for human gene therapy...