Amander Clark


Affiliation: University of California
Country: USA


  1. Chen D, Liu W, Zimmerman J, Pastor W, Kim R, Hosohama L, et al. The TFAP2C-Regulated OCT4 Naive Enhancer Is Involved in Human Germline Formation. Cell Rep. 2018;25:3591-3602.e5 pubmed publisher
    ..Using CRISPR/Cas9, we show that deleting the TFAP2C-bound naive enhancer at the OCT4 locus (also called POU5F1) results in impaired OCT4 expression and a negative effect on hPGCLC identity. ..
  2. Sosa E, Chen D, Rojas E, Hennebold J, Peters K, Wu Z, et al. Differentiation of primate primordial germ cell-like cells following transplantation into the adult gonadal niche. Nat Commun. 2018;9:5339 pubmed publisher
  3. Gell J, Clark A. Restoring Fertility with Human Induced Pluripotent Stem Cells: Are We There Yet?. Cell Stem Cell. 2018;23:777-779 pubmed publisher
    ..For those young women who no longer make functional eggs, the hope of conceiving a biological child just got one step closer with a recent publication in Science from Yamashiro et al. (2018). ..
  4. Gkountela S, Clark A. A big surprise in the little zygote: the curious business of losing methylated cytosines. Cell Stem Cell. 2014;15:393-394 pubmed publisher
    ..Recent investigations have sought to understand the mechanisms of how 5mCs are added and in particular how 5mCs are removed from DNA during embryogenesis. ..
  5. Clark A. DNA methylation remodeling in vitro and in vivo. Curr Opin Genet Dev. 2015;34:82-7 pubmed publisher
    ..Taken together understanding DNA methylation remodeling is critical for understanding the epigenetic foundations of life, and the quality of stem cells for regenerative medicine. ..
  6. Oliveros Etter M, Li Z, Nee K, Hosohama L, Hargan Calvopina J, Lee S, et al. PGC Reversion to Pluripotency Involves Erasure of DNA Methylation from Imprinting Control Centers followed by Locus-Specific Re-methylation. Stem Cell Reports. 2015;5:337-49 pubmed publisher
    ..Therefore, launching PGC differentiation from ESC lines with appropriately methylated ICCs is critical to the generation of germline cells that recapitulate endogenous ICC erasure. ..
  7. Gkountela S, Zhang K, Shafiq T, Liao W, Hargan Calvopiña J, Chen P, et al. DNA Demethylation Dynamics in the Human Prenatal Germline. Cell. 2015;161:1425-36 pubmed publisher
    ..Taken together, the human germline serves as a valuable in vivo tool for monitoring the epigenome of cells that have emerged from a global DNA demethylation event. ..
  8. Tao Y, Yen M, Chitiashvili T, Nakano H, Kim R, Hosohama L, et al. TRIM28-Regulated Transposon Repression Is Required for Human Germline Competency and Not Primed or Naive Human Pluripotency. Stem Cell Reports. 2018;10:243-256 pubmed publisher
    ..Taken together, our study shows that abnormal TE expression is tolerated by self-renewing human pluripotent cells, whereas germline competency is not. ..
  9. Gell J, Zhao J, Chen D, Hunt T, Clark A. PRDM14 is expressed in germ cell tumors with constitutive overexpression altering human germline differentiation and proliferation. Stem Cell Res. 2018;27:46-56 pubmed publisher
    ..Instead, we show that elevated PRDM14 in PGCLCs causes proliferation and differentiation defects in the germline. ..

More Information


  1. Pastor W, Liu W, Chen D, Ho J, Kim R, Hunt T, et al. TFAP2C regulates transcription in human naive pluripotency by opening enhancers. Nat Cell Biol. 2018;20:553-564 pubmed publisher
    ..Taken together, TFAP2C establishes and maintains naive human pluripotency and regulates OCT4 expression by mechanisms that are distinct from mouse. ..
  2. Li Z, Yu J, Hosohama L, Nee K, Gkountela S, Chaudhari S, et al. The Sm protein methyltransferase PRMT5 is not required for primordial germ cell specification in mice. EMBO J. 2015;34:748-58 pubmed publisher
    ..We show that PRMT5 regulates gene expression by promoting methylation of the Sm spliceosomal proteins and significantly altering the spliced repertoire of RNAs in mammalian embryonic cells and primordial cells. ..
  3. Hargan Calvopina J, Taylor S, Cook H, Hu Z, Lee S, Yen M, et al. Stage-Specific Demethylation in Primordial Germ Cells Safeguards against Precocious Differentiation. Dev Cell. 2016;39:75-86 pubmed publisher
    ..Therefore, bypassing stage-specific DNA demethylation has significant consequences for progenitor germ cell differentiation and the ability to transmit DNA from parent to offspring. ..
  4. Sosa E, Kim R, Rojas E, Hosohama L, Hennebold J, Orwig K, et al. An integration-free, virus-free rhesus macaque induced pluripotent stem cell line (riPSC89) from embryonic fibroblasts. Stem Cell Res. 2016;17:444-447 pubmed publisher
    ..The riPSC89 line may be a useful non-human primate resource to uncover developmental origins of disease, or used as a basic model to understand lineage specification in the primate embryo. ..
  5. Chen D, Gell J, Tao Y, Sosa E, Clark A. Modeling human infertility with pluripotent stem cells. Stem Cell Res. 2017;21:187-192 pubmed publisher
  6. Clark A, Gkountela S, Chen D, Liu W, Sosa E, Sukhwani M, et al. Primate Primordial Germ Cells Acquire Transplantation Potential by Carnegie Stage 23. Stem Cell Reports. 2017;9:329-341 pubmed publisher
    ..These results provide a blueprint for differentiating pluripotent stem cells to transplantable PGC-like cells in a species that is amenable to transplantation and fertility studies. ..
  7. Sosa E, Kim R, Rojas E, Hosohama L, Hennebold J, Orwig K, et al. An integration-free, virus-free rhesus macaque induced pluripotent stem cell line (riPSC90) from embryonic fibroblasts. Stem Cell Res. 2017;21:5-8 pubmed publisher
    ..riPSC90 could be used in parallel with riPSC89, which was derived from REFs cultured from a different rhesus macaque embryo (Sosa et al. 2016). ..
  8. request reprint
    Clark A. The stem cell identity of testicular cancer. Stem Cell Rev. 2007;3:49-59 pubmed
    ..At the conclusion of this review, a model for the molecular events involved in germ cell tumor formation and the relationship between germ cell tumorigenesis and stem cell biology will be presented. ..