Pawel S Ciborowski

Summary

Affiliation: University of Nebraska Medical Center
Country: USA

Publications

  1. pmc Plasma proteomic profiling in HIV-1 infected methamphetamine abusers
    Gwenael Pottiez
    Department of Pharmacology and Experimental Neuroscience, Omaha, Nebraska, United States of America
    PLoS ONE 7:e31031. 2012
  2. pmc Comparison of 4-plex to 8-plex iTRAQ quantitative measurements of proteins in human plasma samples
    Gwenael Pottiez
    Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, Nebraska 68198 5800, USA
    J Proteome Res 11:3774-81. 2012
  3. pmc Immunoreactivity of anti-gelsolin antibodies: implications for biomarker validation
    Nicole Haverland
    Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE 68198, USA
    J Transl Med 8:137. 2010
  4. pmc Biomarkers of HIV-1-associated neurocognitive disorders: challenges of proteomic approaches
    Pawel Ciborowski
    Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE 68198 5800, USA
    Biomark Med 3:771-85. 2009
  5. pmc Biomarkers of HIV-1 associated dementia: proteomic investigation of sera
    Jayme Wiederin
    Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE 68198 5800, USA
    Proteome Sci 7:8. 2009
  6. pmc Investigating the human immunodeficiency virus type 1-infected monocyte-derived macrophage secretome
    Pawel Ciborowski
    Center for Neurovirology and Neurodegenerative Disorders, University of Nebraska Medical Center, Omaha, NE 68198 5800, USA
    Virology 363:198-209. 2007
  7. ncbi request reprint Human immunodeficiency virus-mononuclear phagocyte interactions: emerging avenues of biomarker discovery, modes of viral persistence and disease pathogenesis
    Pawel Ciborowski
    Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE 68198 5800, USA
    Curr HIV Res 4:279-91. 2006
  8. pmc Plasma proteomic analysis of simian immunodeficiency virus infection of rhesus macaques
    Jayme L Wiederin
    University of Nebraska Medical Center, Omaha, Nebraska 68198 5880, USA
    J Proteome Res 9:4721-31. 2010
  9. ncbi request reprint Genomic and proteomic microglial profiling: pathways for neuroprotective inflammatory responses following nerve fragment clearance and activation
    Jason G Glanzer
    Center for Neurovirology and Neurodegenerative Disorders, University of Nebraska Medical Center, Omaha, Nebraska 68198 5880, USA
    J Neurochem 102:627-45. 2007
  10. pmc HIV-1 infected monocyte-derived macrophages affect the human brain microvascular endothelial cell proteome: new insights into blood-brain barrier dysfunction for HIV-1-associated dementia
    Mary Ricardo-Dukelow
    Center for Neurovirology and Neurodegenerative Disorders, Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE 68198 5215, USA
    J Neuroimmunol 185:37-46. 2007

Research Grants

  1. Macrophage Protein Fingerprints in HIV-1 Dementia
    Pawel Ciborowski; Fiscal Year: 2006

Collaborators

Detail Information

Publications22

  1. pmc Plasma proteomic profiling in HIV-1 infected methamphetamine abusers
    Gwenael Pottiez
    Department of Pharmacology and Experimental Neuroscience, Omaha, Nebraska, United States of America
    PLoS ONE 7:e31031. 2012
    ..These proteins were involved in complement, coagulation pathways and oxidative stress. Continuous METH use is an unstable condition, altering levels of a number of plasma proteins...
  2. pmc Comparison of 4-plex to 8-plex iTRAQ quantitative measurements of proteins in human plasma samples
    Gwenael Pottiez
    Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, Nebraska 68198 5800, USA
    J Proteome Res 11:3774-81. 2012
    ..0 software. We observed that 8-plex tagging provides more consistent ratios than 4-plex without compromising protein identification, thus allowing investigation of eight experimental conditions in one analytical experiment...
  3. pmc Immunoreactivity of anti-gelsolin antibodies: implications for biomarker validation
    Nicole Haverland
    Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE 68198, USA
    J Transl Med 8:137. 2010
    ..Gelsolin is one such biomarker candidate in HIV-associated neurocognitive disorders...
  4. pmc Biomarkers of HIV-1-associated neurocognitive disorders: challenges of proteomic approaches
    Pawel Ciborowski
    Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE 68198 5800, USA
    Biomark Med 3:771-85. 2009
    ....
  5. pmc Biomarkers of HIV-1 associated dementia: proteomic investigation of sera
    Jayme Wiederin
    Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE 68198 5800, USA
    Proteome Sci 7:8. 2009
    ....
  6. pmc Investigating the human immunodeficiency virus type 1-infected monocyte-derived macrophage secretome
    Pawel Ciborowski
    Center for Neurovirology and Neurodegenerative Disorders, University of Nebraska Medical Center, Omaha, NE 68198 5800, USA
    Virology 363:198-209. 2007
    ..This study, using a unique proteomics platform, provides novel insights into virus-host cell interactions that likely affect the functional role of macrophages in HIV disease...
  7. ncbi request reprint Human immunodeficiency virus-mononuclear phagocyte interactions: emerging avenues of biomarker discovery, modes of viral persistence and disease pathogenesis
    Pawel Ciborowski
    Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE 68198 5800, USA
    Curr HIV Res 4:279-91. 2006
    ....
  8. pmc Plasma proteomic analysis of simian immunodeficiency virus infection of rhesus macaques
    Jayme L Wiederin
    University of Nebraska Medical Center, Omaha, Nebraska 68198 5880, USA
    J Proteome Res 9:4721-31. 2010
    ..Observed changes in plasma proteins expression likely represent a compensatory host response to persistent viral infection...
  9. ncbi request reprint Genomic and proteomic microglial profiling: pathways for neuroprotective inflammatory responses following nerve fragment clearance and activation
    Jason G Glanzer
    Center for Neurovirology and Neurodegenerative Disorders, University of Nebraska Medical Center, Omaha, Nebraska 68198 5880, USA
    J Neurochem 102:627-45. 2007
    ..Most importantly, this molecular and biochemical signature supports a broad range of microglial functions for debris clearance and promotion of neural repair after injury...
  10. pmc HIV-1 infected monocyte-derived macrophages affect the human brain microvascular endothelial cell proteome: new insights into blood-brain barrier dysfunction for HIV-1-associated dementia
    Mary Ricardo-Dukelow
    Center for Neurovirology and Neurodegenerative Disorders, Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE 68198 5215, USA
    J Neuroimmunol 185:37-46. 2007
    ..Results were validated by Western blot analysis. We conclude that HIV-1-infected MDM affect the HBMEC proteome and, in this way, affect BBB dysfunction and the development of HIV-1 CNS disease...
  11. ncbi request reprint Proteomic fingerprints distinguish microglia, bone marrow, and spleen macrophage populations
    Yoshimi Enose
    Center for Neurovirology and Neurodegenerative Disorders, University of Nebraska Medical Center, Omaha, Nebraska 68198 5880, USA
    Glia 51:161-72. 2005
    ..The results provide a unique means to distinguish microglia from other MP subtypes...
  12. ncbi request reprint Diminished matrix metalloproteinase 9 secretion in human immunodeficiency virus-infected mononuclear phagocytes: modulation of innate immunity and implications for neurological disease
    Pawel Ciborowski
    Center for Neurovirology and Neurodegenerative Disorders, 985215 Nebraska Medical Center, University of Nebraska Medical Center, Omaha, NE 68198 5215, U SA
    J Neuroimmunol 157:11-6. 2004
    ..These data further demonstrate immunoregulatory activities of HIV-1-infected MDM providing unique insights into cellular function in disease...
  13. ncbi request reprint Cerebrospinal fluid proteomic profiling of HIV-1-infected patients with cognitive impairment
    Wojciech Rozek
    Center for Neurovirology and Neurodegenerative Disorders, Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska 68198 5800, USA
    J Proteome Res 6:4189-99. 2007
    ..We posit that these proteins, alone or together, are potential HAD biomarkers...
  14. ncbi request reprint Cytoskeletal protein transformation in HIV-1-infected macrophage giant cells
    Irena Kadiu
    Laboratory of Neuroregeneration, Department of Pharmacology and Experimental Neuroscience, Center for Neurovirology and Neurodegenerative Disorders, University of Nebraska Medical Center, Omaha 68198, USA
    J Immunol 178:6404-15. 2007
    ..Such events favor the virus over the host cell and provide insights into macrophage defense mechanisms used to contain viral growth and how they may be affected during progressive HIV-1 infection...
  15. ncbi request reprint Proteomic fingerprinting of HIV-1-infected human monocyte-derived macrophages: a preliminary report
    Kimberly A Carlson
    Center for Neurovirology and Neurodegenerative Disorders, Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE 68198 5215, USA
    J Neuroimmunol 147:35-42. 2004
    ..Moreover, this evolving technology can be utilized to unravel changes in immune activities initiated by interactions between virus, environmental cues and drugs of abuse...
  16. pmc Pressure-assisted sample preparation for proteomic analysis
    Pawel P Olszowy
    Department of Pharmacology and Experimental Neuroscience, College of Medicine, University of Nebraska Medical Center, Omaha, NE 68198, USA
    Anal Biochem 438:67-72. 2013
    ..PCT was also found to allow for unambiguous control of digestion parameters and to provide a high yield of sequence coverage compared with atmospheric pressure...
  17. pmc HIV-1 transforms the monocyte plasma membrane proteome
    Irena Kadiu
    Department of Pharmacology and Experimental Neuroscience, Center for Neurovirology and Neurodegenerative Disorders, University of Nebraska Medical Center, Omaha, NE 68198 5880, USA
    Cell Immunol 258:44-58. 2009
    ..These results demonstrate that HIV-1 uniquely affects monocyte activation and oxidative stress. These do not affect viral infection dynamics but are linked to stress-induced cell death...
  18. pmc Calpain and proteasomal regulation of antiretroviral zinc finger protein OTK18 in human macrophages: visualization in live cells by intramolecular FRET
    Lindsey B Martinez
    Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE 68198 5880, USA
    J Neuroimmune Pharmacol 4:116-28. 2009
    ..These data suggest that calpain and proteasome are involved in OTK18 endoproteolysis and degradation. Additionally, intramolecular FRET has proven to be a useful tool for monitoring the processing in live cells...
  19. pmc HIV-1-infected astrocytes and the microglial proteome
    Tong Wang
    Center for Neurovirology and Neurodegenerative Disorders, University of Nebraska Medical Center, Omaha, NE 68198 5880, USA
    J Neuroimmune Pharmacol 3:173-86. 2008
    ..The observations confirmed the potential of astrocytes to influence the neuropathogenesis of HIV-1 infection by specifically altering the neurotoxic potential of infected microglia and regulating viral maturation...
  20. pmc Proteomic modeling for HIV-1 infected microglia-astrocyte crosstalk
    Tong Wang
    Center for Neurovirology and Neurodegenerative Disorders, Department of Pharmacology and Experimental Neuroscience, Omaha, Nebraska, United States of America
    PLoS ONE 3:e2507. 2008
    ..In contrast, astrocytes can affect disease by modulating the nervous system's microenvironment. Interestingly, little is known how astrocytes communicate with MP to influence disease...
  21. pmc Nitrated alpha-synuclein and microglial neuroregulatory activities
    Ashley D Reynolds
    Center for Neurovirology and Neurodegenerative Disorders, University of Nebraska Medical Center, Omaha, NE 68198, USA
    J Neuroimmune Pharmacol 3:59-74. 2008
    ..Inhibition of cathepsin B attenuated, in part, N-alpha-syn microglial neurotoxicity. These data support multifaceted microglia functions in PD-associated neurodegeneration...
  22. ncbi request reprint Nitrated alpha-synuclein-activated microglial profiling for Parkinson's disease
    Ashley D Reynolds
    Center for Neurovirology and Neurodegenerative Disorders, University of Nebraska Medical Center, Omaha, Nebraska 68198 5880, USA
    J Neurochem 104:1504-25. 2008
    ..Taken together, the transcriptome and proteome of nitrated alpha-syn activated microglia, shown herein, provide new potential insights into disease mechanisms...

Research Grants1

  1. Macrophage Protein Fingerprints in HIV-1 Dementia
    Pawel Ciborowski; Fiscal Year: 2006
    ..The long-term goal of this aim is to uncover biomarkers specific for HAD and to see if they are based on products of immune competent virus-infected MDM as demonstrated in aim 1. ..