Pawel Ciborowski

Summary

Affiliation: University of Nebraska Medical Center
Country: USA

Publications

  1. ncbi request reprint Diminished matrix metalloproteinase 9 secretion in human immunodeficiency virus-infected mononuclear phagocytes: modulation of innate immunity and implications for neurological disease
    Pawel Ciborowski
    Center for Neurovirology and Neurodegenerative Disorders, 985215 Nebraska Medical Center, University of Nebraska Medical Center, Omaha, NE 68198 5215, U SA
    J Neuroimmunol 157:11-6. 2004
  2. pmc HIV-1-infected astrocytes and the microglial proteome
    Tong Wang
    Center for Neurovirology and Neurodegenerative Disorders, University of Nebraska Medical Center, Omaha, NE 68198 5880, USA
    J Neuroimmune Pharmacol 3:173-86. 2008
  3. pmc Nitrated alpha-synuclein and microglial neuroregulatory activities
    Ashley D Reynolds
    Center for Neurovirology and Neurodegenerative Disorders, University of Nebraska Medical Center, Omaha, NE 68198, USA
    J Neuroimmune Pharmacol 3:59-74. 2008
  4. doi request reprint Spatial learning impairment, enhanced CDK5/p35 activity, and downregulation of NMDA receptor expression in transgenic mice expressing tau-tubulin kinase 1
    Shinji Sato
    Department of Pharmacology and Experimental Neuroscience and Center for Neurovirology and Neurodegenerative Disorders, University of Nebraska Medical Center, Omaha, Nebraska 68198 5880, USA
    J Neurosci 28:14511-21. 2008
  5. pmc HIV-1 infected monocyte-derived macrophages affect the human brain microvascular endothelial cell proteome: new insights into blood-brain barrier dysfunction for HIV-1-associated dementia
    Mary Ricardo-Dukelow
    Center for Neurovirology and Neurodegenerative Disorders, Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE 68198 5215, USA
    J Neuroimmunol 185:37-46. 2007
  6. pmc HIV-1 transforms the monocyte plasma membrane proteome
    Irena Kadiu
    Department of Pharmacology and Experimental Neuroscience, Center for Neurovirology and Neurodegenerative Disorders, University of Nebraska Medical Center, Omaha, NE 68198 5880, USA
    Cell Immunol 258:44-58. 2009
  7. ncbi request reprint Cytoskeletal protein transformation in HIV-1-infected macrophage giant cells
    Irena Kadiu
    Laboratory of Neuroregeneration, Department of Pharmacology and Experimental Neuroscience, Center for Neurovirology and Neurodegenerative Disorders, University of Nebraska Medical Center, Omaha 68198, USA
    J Immunol 178:6404-15. 2007
  8. ncbi request reprint Cerebrospinal fluid proteomic profiling of HIV-1-infected patients with cognitive impairment
    Wojciech Rozek
    Center for Neurovirology and Neurodegenerative Disorders, Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska 68198 5800, USA
    J Proteome Res 6:4189-99. 2007
  9. pmc Proteomic modeling for HIV-1 infected microglia-astrocyte crosstalk
    Tong Wang
    Center for Neurovirology and Neurodegenerative Disorders, Department of Pharmacology and Experimental Neuroscience, Omaha, Nebraska, United States of America
    PLoS ONE 3:e2507. 2008
  10. pmc Comparison of 4-plex to 8-plex iTRAQ quantitative measurements of proteins in human plasma samples
    Gwenael Pottiez
    Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, Nebraska 68198 5800, USA
    J Proteome Res 11:3774-81. 2012

Collaborators

Detail Information

Publications26

  1. ncbi request reprint Diminished matrix metalloproteinase 9 secretion in human immunodeficiency virus-infected mononuclear phagocytes: modulation of innate immunity and implications for neurological disease
    Pawel Ciborowski
    Center for Neurovirology and Neurodegenerative Disorders, 985215 Nebraska Medical Center, University of Nebraska Medical Center, Omaha, NE 68198 5215, U SA
    J Neuroimmunol 157:11-6. 2004
    ..These data further demonstrate immunoregulatory activities of HIV-1-infected MDM providing unique insights into cellular function in disease...
  2. pmc HIV-1-infected astrocytes and the microglial proteome
    Tong Wang
    Center for Neurovirology and Neurodegenerative Disorders, University of Nebraska Medical Center, Omaha, NE 68198 5880, USA
    J Neuroimmune Pharmacol 3:173-86. 2008
    ..The observations confirmed the potential of astrocytes to influence the neuropathogenesis of HIV-1 infection by specifically altering the neurotoxic potential of infected microglia and regulating viral maturation...
  3. pmc Nitrated alpha-synuclein and microglial neuroregulatory activities
    Ashley D Reynolds
    Center for Neurovirology and Neurodegenerative Disorders, University of Nebraska Medical Center, Omaha, NE 68198, USA
    J Neuroimmune Pharmacol 3:59-74. 2008
    ..Inhibition of cathepsin B attenuated, in part, N-alpha-syn microglial neurotoxicity. These data support multifaceted microglia functions in PD-associated neurodegeneration...
  4. doi request reprint Spatial learning impairment, enhanced CDK5/p35 activity, and downregulation of NMDA receptor expression in transgenic mice expressing tau-tubulin kinase 1
    Shinji Sato
    Department of Pharmacology and Experimental Neuroscience and Center for Neurovirology and Neurodegenerative Disorders, University of Nebraska Medical Center, Omaha, Nebraska 68198 5880, USA
    J Neurosci 28:14511-21. 2008
    ..These data suggest that TTBK1 in AD brain may be one of the underlying mechanisms inducing CDK5 and calpain activation, NR2B downregulation, and subsequent memory dysfunction...
  5. pmc HIV-1 infected monocyte-derived macrophages affect the human brain microvascular endothelial cell proteome: new insights into blood-brain barrier dysfunction for HIV-1-associated dementia
    Mary Ricardo-Dukelow
    Center for Neurovirology and Neurodegenerative Disorders, Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE 68198 5215, USA
    J Neuroimmunol 185:37-46. 2007
    ..Results were validated by Western blot analysis. We conclude that HIV-1-infected MDM affect the HBMEC proteome and, in this way, affect BBB dysfunction and the development of HIV-1 CNS disease...
  6. pmc HIV-1 transforms the monocyte plasma membrane proteome
    Irena Kadiu
    Department of Pharmacology and Experimental Neuroscience, Center for Neurovirology and Neurodegenerative Disorders, University of Nebraska Medical Center, Omaha, NE 68198 5880, USA
    Cell Immunol 258:44-58. 2009
    ..These results demonstrate that HIV-1 uniquely affects monocyte activation and oxidative stress. These do not affect viral infection dynamics but are linked to stress-induced cell death...
  7. ncbi request reprint Cytoskeletal protein transformation in HIV-1-infected macrophage giant cells
    Irena Kadiu
    Laboratory of Neuroregeneration, Department of Pharmacology and Experimental Neuroscience, Center for Neurovirology and Neurodegenerative Disorders, University of Nebraska Medical Center, Omaha 68198, USA
    J Immunol 178:6404-15. 2007
    ..Such events favor the virus over the host cell and provide insights into macrophage defense mechanisms used to contain viral growth and how they may be affected during progressive HIV-1 infection...
  8. ncbi request reprint Cerebrospinal fluid proteomic profiling of HIV-1-infected patients with cognitive impairment
    Wojciech Rozek
    Center for Neurovirology and Neurodegenerative Disorders, Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska 68198 5800, USA
    J Proteome Res 6:4189-99. 2007
    ..We posit that these proteins, alone or together, are potential HAD biomarkers...
  9. pmc Proteomic modeling for HIV-1 infected microglia-astrocyte crosstalk
    Tong Wang
    Center for Neurovirology and Neurodegenerative Disorders, Department of Pharmacology and Experimental Neuroscience, Omaha, Nebraska, United States of America
    PLoS ONE 3:e2507. 2008
    ..In contrast, astrocytes can affect disease by modulating the nervous system's microenvironment. Interestingly, little is known how astrocytes communicate with MP to influence disease...
  10. pmc Comparison of 4-plex to 8-plex iTRAQ quantitative measurements of proteins in human plasma samples
    Gwenael Pottiez
    Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, Nebraska 68198 5800, USA
    J Proteome Res 11:3774-81. 2012
    ..0 software. We observed that 8-plex tagging provides more consistent ratios than 4-plex without compromising protein identification, thus allowing investigation of eight experimental conditions in one analytical experiment...
  11. pmc Pulsed stable isotope labeling of amino acids in cell culture uncovers the dynamic interactions between HIV-1 and the monocyte-derived macrophage
    Stephanie D Kraft-Terry
    Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, Nebraska 68198 5880, USA
    J Proteome Res 10:2852-62. 2011
    ..We conclude that HIV-1 regulates a range of host MDM proteins that affect its survival and abilities to contain infection...
  12. pmc Proteomic analyses of monocytes obtained from Hispanic women with HIV-associated dementia show depressed antioxidants
    Stephanie Kraft-Terry
    Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE, USA
    Proteomics Clin Appl 4:706-14. 2010
    ..Although activated monocyte phenotypes emerge in disease, the functional correlates of these cells remain unresolved...
  13. pmc Investigating the human immunodeficiency virus type 1-infected monocyte-derived macrophage secretome
    Pawel Ciborowski
    Center for Neurovirology and Neurodegenerative Disorders, University of Nebraska Medical Center, Omaha, NE 68198 5800, USA
    Virology 363:198-209. 2007
    ..This study, using a unique proteomics platform, provides novel insights into virus-host cell interactions that likely affect the functional role of macrophages in HIV disease...
  14. pmc Nitrated alpha-synuclein immunity accelerates degeneration of nigral dopaminergic neurons
    Eric J Benner
    Center for Neurovirology and Neurodegenerative Disorders, Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, Nebraska, USA
    PLoS ONE 3:e1376. 2008
    ..We reasoned that PD-associated oxidative protein modifications create novel antigenic epitopes capable of peripheral adaptive T cell responses that could affect nigrostriatal degeneration...
  15. ncbi request reprint Nitrated alpha-synuclein-activated microglial profiling for Parkinson's disease
    Ashley D Reynolds
    Center for Neurovirology and Neurodegenerative Disorders, University of Nebraska Medical Center, Omaha, Nebraska 68198 5880, USA
    J Neurochem 104:1504-25. 2008
    ..Taken together, the transcriptome and proteome of nitrated alpha-syn activated microglia, shown herein, provide new potential insights into disease mechanisms...
  16. pmc Biomarkers of HIV-1-associated neurocognitive disorders: challenges of proteomic approaches
    Pawel Ciborowski
    Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE 68198 5800, USA
    Biomark Med 3:771-85. 2009
    ....
  17. pmc Changes in the plasma proteome follows chronic opiate administration in simian immunodeficiency virus infected rhesus macaques
    Jayme L Wiederin
    Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, 985800 Nebraska Medical Center, Omaha, NE 68198 5880, USA
    Drug Alcohol Depend 120:105-12. 2012
    ..To this end, we investigated the effect of opiate administration on the plasma proteome of Indian-strain rhesus monkeys infected with simian immunodeficiency virus (SIV) strain smm9...
  18. pmc Calpain and proteasomal regulation of antiretroviral zinc finger protein OTK18 in human macrophages: visualization in live cells by intramolecular FRET
    Lindsey B Martinez
    Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE 68198 5880, USA
    J Neuroimmune Pharmacol 4:116-28. 2009
    ..These data suggest that calpain and proteasome are involved in OTK18 endoproteolysis and degradation. Additionally, intramolecular FRET has proven to be a useful tool for monitoring the processing in live cells...
  19. pmc Elucidating protein inter- and intramolecular interacting domains using chemical cross-linking and matrix-assisted laser desorption ionization-time of flight/time of flight mass spectrometry
    Gwenael Pottiez
    Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE 68198 5800, USA
    Anal Biochem 421:712-8. 2012
    ....
  20. pmc Biomarkers of HIV-1 associated dementia: proteomic investigation of sera
    Jayme Wiederin
    Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE 68198 5800, USA
    Proteome Sci 7:8. 2009
    ....
  21. pmc Immunoreactivity of anti-gelsolin antibodies: implications for biomarker validation
    Nicole Haverland
    Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE 68198, USA
    J Transl Med 8:137. 2010
    ..Gelsolin is one such biomarker candidate in HIV-associated neurocognitive disorders...
  22. ncbi request reprint Human immunodeficiency virus-mononuclear phagocyte interactions: emerging avenues of biomarker discovery, modes of viral persistence and disease pathogenesis
    Pawel Ciborowski
    Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE 68198 5800, USA
    Curr HIV Res 4:279-91. 2006
    ....
  23. ncbi request reprint Proteomic fingerprinting of HIV-1-infected human monocyte-derived macrophages: a preliminary report
    Kimberly A Carlson
    Center for Neurovirology and Neurodegenerative Disorders, Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE 68198 5215, USA
    J Neuroimmunol 147:35-42. 2004
    ..Moreover, this evolving technology can be utilized to unravel changes in immune activities initiated by interactions between virus, environmental cues and drugs of abuse...
  24. pmc Sera proteomic biomarker profiling in HIV-1 infected subjects with cognitive impairment
    Wojciech Rozek
    Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE, USA Department of Virology, National Veterinary Research Institute, Pulawy, Poland
    Proteomics Clin Appl 2:1498-507. 2008
    ..Differential expression of two proteins was validated by Western blot tests identifying afamin and ceruloplasmin as a potential biomarkers for CI associated with advanced HIV-1 infection...
  25. pmc CSF proteomic fingerprints for HIV-associated cognitive impairment
    Juliana Pérez Laspiur
    Specialized Neurosciences Research Program, University of Puerto Rico Medical Sciences Campus, San Juan, Puerto Rico
    J Neuroimmunol 192:157-70. 2007
    ..This work highlights, in a preliminary manner, the utility of proteomic profiling for biomarker discovery for HIV-1 associated cognitive dysfunction...
  26. ncbi request reprint The Scl1 protein of M6-type group A Streptococcus binds the human complement regulatory protein, factor H, and inhibits the alternative pathway of complement
    Clayton C Caswell
    Department of Microbiology, Immunology, and Cell Biology, School of Medicine, West Virginia University, Morgantown, WV 26506, USA
    Mol Microbiol 67:584-96. 2008
    ..In conclusion, we have identified a functional interaction between Scl1 and plasma FH, which may contribute to GAS evasion of complement-mediated opsonization and phagocytosis...