Research Topics
Species | Charleen ChuSummaryAffiliation: University of Pittsburgh Country: USA Publications
Research Grants
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Detail Information
Publications
Role of autophagy in G2019S-LRRK2-associated neurite shortening in differentiated SH-SY5Y cellsEdward D Plowey
Department of Pathology, Division of Neuropathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
J Neurochem 105:1048-56. 2008..These results indicate an active role for autophagy in neurite remodeling induced by pathogenic mutation of LRRK2...- Location, location, location: altered transcription factor trafficking in neurodegenerationCharleen T Chu
Department of Pathology, Division of Neuropathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
J Neuropathol Exp Neurol 66:873-83. 2007..Studying the pathophysiologic mechanisms regulating transcription factor localization should facilitate strategies to bypass deficits and restore adaptive neuroprotective transcriptional responses... 
Reactive oxygen/nitrogen species at the fulcrum of life-death decisions: a commentary on "peroxynitrite transforms nerve growth factor into an apoptotic factor for motor neurons"Charleen T Chu
Department of Pathology, University of Pittsburgh, Pittsburgh, PA 15213, USA
Free Radic Biol Med 41:1629-31. 2006- Beclin 1-independent pathway of damage-induced mitophagy and autophagic stress: implications for neurodegeneration and cell deathCharleen T Chu
Department of Pathology, Center for Neuroscience, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
Autophagy 3:663-6. 2007.... - Autophagic stress in neuronal injury and diseaseCharleen T Chu
Department of Pathology Division of Neuropathology, Department of Ophthalmology, Center for Neuroscience CNUP, Pittsburgh Institute for Neurodegenerative Diseases PIND, University of Pittsburgh School of Medicine, Pennsylvania, USA
J Neuropathol Exp Neurol 65:423-32. 2006..quot; The concept of autophagic stress may reconcile apparently conflicting roles of autophagy-related processes in adaptive, homeostatic responses and in pathways of neurodegeneration and cell death... - Apoptosis inducing factor mediates caspase-independent 1-methyl-4-phenylpyridinium toxicity in dopaminergic cellsCharleen T Chu
Department of Pathology, Division of Neuropathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
J Neurochem 94:1685-95. 2005..Studies of primary mouse midbrain cultures further supported a role for AIF in caspase-independent cell death in MPP+-treated dopaminergic neurons... - A pivotal role for PINK1 and autophagy in mitochondrial quality control: implications for Parkinson diseaseCharleen T Chu
Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
Hum Mol Genet 19:R28-37. 2010.... - Tickled PINK1: mitochondrial homeostasis and autophagy in recessive ParkinsonismCharleen T Chu
Department of Pathology Division of Neuropathology, Center for Neuroscience and McGowan Institute for Regenerative Medicine, University of Pittsburgh School of Medicine, 200 Lothrop St, Pittsburgh, PA, USA
Biochim Biophys Acta 1802:20-8. 2010.... - Eaten alive: autophagy and neuronal cell death after hypoxia-ischemiaCharleen T Chu
Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
Am J Pathol 172:284-7. 2008 - Functional repression of cAMP response element in 6-hydroxydopamine-treated neuronal cellsElisabeth M Chalovich
Department of Pathology, Division of Neuropathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
J Biol Chem 281:17870-81. 2006.... - Regulation of autophagy by extracellular signal-regulated protein kinases during 1-methyl-4-phenylpyridinium-induced cell deathJian Hui Zhu
Department of Pathology Division of Neuropathology, Pittsburgh Institute for Neurodegenerative Diseases, Center for Biologic Imaging, Pittsburgh, PA, USA
Am J Pathol 170:75-86. 2007..Moreover, pathological stimulation of beclin 1-independent autophagy is associated with neuronal cell death... - Peroxidase mechanism of lipid-dependent cross-linking of synuclein with cytochrome C: protection against apoptosis versus delayed oxidative stress in Parkinson diseaseHulya Bayir
Center for Free Radical and Antioxidant Health, Pittsburgh Institute for Neurodegenerative Diseases, University of Pittsburgh, Pittsburgh, Pennsylvania 15219 3130, USA
J Biol Chem 284:15951-69. 2009..This immediate protective function of Syn is associated with the formation of the peroxidase complex representing a source of oxidative stress and postponed damage... - Autophagy, mitochondria and cell death in lysosomal storage diseasesKirill Kiselyov
Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
Autophagy 3:259-62. 2007..The resulting accumulation of dysfunctional mitochondria showing impaired Ca2+ buffering increases the vulnerability of the cells to pro-apoptotic signals... - Loss of PINK1 function promotes mitophagy through effects on oxidative stress and mitochondrial fissionRuben K Dagda
Department of Pathology and Center for Neuroscience, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
J Biol Chem 284:13843-55. 2009..Furthermore, PINK1 and Parkin may cooperate through different mechanisms to maintain mitochondrial homeostasis... - A novel transferrin/TfR2-mediated mitochondrial iron transport system is disrupted in Parkinson's diseasePier Giorgio Mastroberardino
Department of Neurology, University of Pittsburgh, 3501 Fifth Avenue, Pittsburgh, PA 15260, USA
Neurobiol Dis 34:417-31. 2009..Thus, we have discovered a novel mitochondrial iron transport system that goes awry in PD, and which may provide a new target for therapeutic intervention... - Mitochondrial quality control: insights on how Parkinson's disease related genes PINK1, parkin, and Omi/HtrA2 interact to maintain mitochondrial homeostasisRuben K Dagda
Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
J Bioenerg Biomembr 41:473-9. 2009..We propose that PINK1, Omi/HtrA2 and parkin participate at different levels in mitochondrial quality control, converging through some overlapping and some distinct steps to maintain a common phenotype of healthy mitochondrial networks... - Role of reactive oxygen species in extracellular signal-regulated protein kinase phosphorylation and 6-hydroxydopamine cytotoxicityScott M Kulich
Department of Pathology, Division of Neuropathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
J Biosci 28:83-9. 2003..This correlation between antioxidant protection and inhibition of 6-OHDA-induced sustained ERK phosphorylation suggests that redox regulation of ERK signalling cascades may contribute to neuronal toxicity... - Oxidative neuronal injury. The dark side of ERK1/2Charleen T Chu
Department of Pathology, Division of Neuropathology, University of Pittsburgh School of Medicine, Room A 516 UPMC Presbyterian, 200 Lothrop Street, Pittsburgh, PA 15213, USA
Eur J Biochem 271:2060-6. 2004.... - Mitochondrially localized ERK2 regulates mitophagy and autophagic cell stress: implications for Parkinson's diseaseRuben K Dagda
Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
Autophagy 4:770-82. 2008..These data indicate that mitochondrial localization of ERK2 activity is sufficient to recapitulate the effects of 6-OHDA on mitophagy and autophagic cell death... - Localization of phosphorylated ERK/MAP kinases to mitochondria and autophagosomes in Lewy body diseasesJian Hui Zhu
Department of Pathology, Division of Neuropathology, University of Pittsburgh, PA, USA
Brain Pathol 13:473-81. 2003..As mitochondrial pathology may play a pivotal role in Parkinson and other related neurodegenerative diseases, these studies suggest a potential interaction between dysfunctional mitochondria, autophagy, and ERK signaling pathways... 
Mitochondrial dysfunction in Parkinson's diseaseJianhui Zhu
Department of Pathology Division of Neuropathology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
J Alzheimers Dis 20:S325-34. 2010....- Autophagy is increased in mice after traumatic brain injury and is detectable in human brain after trauma and critical illnessRobert S B Clark
Department of Critical Care Medicine, The Safar Center for Resuscitation Research and the Brain Trauma Research Center, Pittsburgh, Pennsylvania 15260, USA
Autophagy 4:88-90. 2008..Taken together, we show that autophagy occurs after both experimental and clinical TBI, and that oxidative stress contributes to overall neuropathology after TBI in mice, at least in part by initiating or influencing autophagy... - Mitochondrial aberrations in mucolipidosis Type IVJohn J Jennings
Department of Biological Science, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
J Biol Chem 281:39041-50. 2006..Deficient Ca2+ homeostasis may represent a common mechanism of degenerative cell death in several lysosomal storage diseases... - Mitochondrial autophagy as a compensatory response to PINK1 deficiencySalvatore J Cherra
Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
Autophagy 5:1213-4. 2009..These data offer additional insights that broaden the spectrum of potential interactions between PINK1 and parkin with respect to the regulation of mitochondrial homeostasis and mitophagy... - Expression of Nrf2 in neurodegenerative diseasesChenere P Ramsey
Department of Pathology, University of Pennsylvania, School of Dental Medicine, Philadelphia, Pennsylvania, USA
J Neuropathol Exp Neurol 66:75-85. 2007..In PD, nuclear localization of Nrf2 is strongly induced, but this response may be insufficient to protect neurons from degeneration... - Mitochondrial kinases in Parkinson's disease: converging insights from neurotoxin and genetic modelsRuben K Dagda
Dept of Pathology, University of Pittsburgh, Pittsburgh, PA 15261, United States of America
Mitochondrion 9:289-98. 2009..Converging mechanisms from different model systems support the concept of common pathways in parkinsonian neurodegeneration that may be amenable to future therapeutic interventions... - Autophagy in neurite injury and neurodegeneration: in vitro and in vivo modelsCharleen T Chu
Department of Pathology, Division of Neuropathology, University of Pittsburgh School of Medicine and Center for Neuroscience, Pittsburgh, Pennsylvania, USA
Methods Enzymol 453:217-49. 2009..Future challenges include application of automated computer-assisted image analysis to the axodendritic tree of individual neurons and improving methods for measuring neuronal autophagic flux... - Kinase signaling cascades in the mitochondrion: a matter of life or deathCraig Horbinski
Division of Neuropathology, Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
Free Radic Biol Med 38:2-11. 2005.... - 6-Hydroxydopamine induces mitochondrial ERK activationScott M Kulich
Department of Pathology, VA Pittsburgh Healthcare System, Pittsburgh, PA 15240, USA
Free Radic Biol Med 43:372-83. 2007.... - Autophagy is increased after traumatic brain injury in mice and is partially inhibited by the antioxidant gamma-glutamylcysteinyl ethyl esterYichen Lai
Department of Critical Care Medicine, The Safar Center for Resuscitation Research and the Brain Trauma Research Center, University of Pittsburgh, Pittsburgh, PA 15260, USA
J Cereb Blood Flow Metab 28:540-50. 2008..Taken together, these data show that autophagy occurs after experimental TBI, and that oxidative stress contributes to overall neuropathology, in part by initiating or influencing autophagy... - Cytoplasmic aggregates of phosphorylated extracellular signal-regulated protein kinases in Lewy body diseasesJian Hui Zhu
Department of Pathology, University of Pittsburgh School of Medicine, 200 Lothrop Street, Pittsburgh, PA 15213, USA
Am J Pathol 161:2087-98. 2002..Moreover, granular cytoplasmic P-ERK was associated with alterations in the distribution of downstream targets such as P-RSK1, but not of P-Elk-1, suggesting functional diversion of ERK-signaling pathways in Lewy body diseases... - Regulation of the autophagy protein LC3 by phosphorylationSalvatore J Cherra
Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
J Cell Biol 190:533-9. 2010..These data demonstrate a role for phosphorylation in regulating LC3 activity... - Starving neurons show sex difference in autophagyLina Du
Department of Critical Care Medicine, Safar Center for Resuscitation Research, University of Pittsburgh School of Medicine, and Children s Hospital of Pittsburgh, Pittsburgh, PA 15260, USA
J Biol Chem 284:2383-96. 2009..Thus, during starvation, neurons from males more readily undergo autophagy and die, whereas neurons from females mobilize fatty acids, accumulate triglycerides, form lipid droplets, and survive longer... - The p75 neurotrophin receptor can induce autophagy and death of cerebellar Purkinje neuronsMaria L Florez-McClure
Department of Pharmacology, University of Colorado Health Sciences Center, and Denver Veterans Affairs Medical Center, Denver, Colorado 80262, USA
J Neurosci 24:4498-509. 2004..The autophagic morphology resembles that found in neurodegenerative disorders, suggesting a potential role for this pathway in neurological disease... - Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotesDaniel J Klionsky
Life Sciences Institute, University of Michigan, Ann Arbor, Michigan 48109 2216, USA
Autophagy 4:151-75. 2008..In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to verify an autophagic response... - Hippocampal long-term potentiation, memory, and longevity in mice that overexpress mitochondrial superoxide dismutaseDaoying Hu
Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX 77030, USA
Neurobiol Learn Mem 87:372-84. 2007..These findings increase our knowledge of the role of mitochondrial superoxide in physiological and pathological processes in the brain...
Research Grants
- Regulation of autophagy in dopaminergic cell deathCharleen T Chu; Fiscal Year: 2010....
- Regulation of PTEN-induced kinase 1 (PINK 1)Charleen Chu; Fiscal Year: 2007..In addition, a group of immunochemical and molecular reagents will have been created that can facilitate the work of other investigators studying mechanisms of Parkinson's and related diseases. ..
- Training in proteomics of novel kinase substrates for neurodegenerationCharleen Chu; Fiscal Year: 2007....
- THE PARKINSONIAN 6 HYDROXYDOPAMINE MODELCharleen Chu; Fiscal Year: 2004....
- Regulation of autophagy in dopaminergic cell deathCharleen Chu; Fiscal Year: 2009....