Charleen Chu

Summary

Affiliation: University of Pittsburgh
Country: USA

Publications

  1. pmc Role of autophagy in G2019S-LRRK2-associated neurite shortening in differentiated SH-SY5Y cells
    Edward D Plowey
    Department of Pathology, Division of Neuropathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    J Neurochem 105:1048-56. 2008
  2. pmc Cardiolipin externalization to the outer mitochondrial membrane acts as an elimination signal for mitophagy in neuronal cells
    Charleen T Chu
    Department of Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    Nat Cell Biol 15:1197-205. 2013
  3. pmc Impaired mitochondrial biogenesis contributes to depletion of functional mitochondria in chronic MPP+ toxicity: dual roles for ERK1/2
    J H Zhu
    Department of Pathology, Division of Neuropathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    Cell Death Dis 3:e312. 2012
  4. pmc Diversity in the regulation of autophagy and mitophagy: lessons from Parkinson's disease
    Charleen T Chu
    Division of Neuropathology, Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    Parkinsons Dis 2011:789431. 2011
  5. pmc Location, location, location: altered transcription factor trafficking in neurodegeneration
    Charleen T Chu
    Department of Pathology, Division of Neuropathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    J Neuropathol Exp Neurol 66:873-83. 2007
  6. pmc Beclin 1-independent pathway of damage-induced mitophagy and autophagic stress: implications for neurodegeneration and cell death
    Charleen T Chu
    Department of Pathology, Center for Neuroscience, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Autophagy 3:663-6. 2007
  7. pmc Tickled PINK1: mitochondrial homeostasis and autophagy in recessive Parkinsonism
    Charleen T Chu
    Department of Pathology Division of Neuropathology, Center for Neuroscience and McGowan Institute for Regenerative Medicine, University of Pittsburgh School of Medicine, 200 Lothrop St, Pittsburgh, PA, USA
    Biochim Biophys Acta 1802:20-8. 2010
  8. ncbi request reprint Reactive oxygen/nitrogen species at the fulcrum of life-death decisions: a commentary on "peroxynitrite transforms nerve growth factor into an apoptotic factor for motor neurons"
    Charleen T Chu
    Department of Pathology, University of Pittsburgh, Pittsburgh, PA 15213, USA
    Free Radic Biol Med 41:1629-31. 2006
  9. pmc A pivotal role for PINK1 and autophagy in mitochondrial quality control: implications for Parkinson disease
    Charleen T Chu
    Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
    Hum Mol Genet 19:R28-37. 2010
  10. pmc Autophagic stress in neuronal injury and disease
    Charleen T Chu
    Department of Pathology Division of Neuropathology, Department of Ophthalmology, Center for Neuroscience CNUP, Pittsburgh Institute for Neurodegenerative Diseases PIND, University of Pittsburgh School of Medicine, Pennsylvania, USA
    J Neuropathol Exp Neurol 65:423-32. 2006

Research Grants

Detail Information

Publications39

  1. pmc Role of autophagy in G2019S-LRRK2-associated neurite shortening in differentiated SH-SY5Y cells
    Edward D Plowey
    Department of Pathology, Division of Neuropathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    J Neurochem 105:1048-56. 2008
    ..These results indicate an active role for autophagy in neurite remodeling induced by pathogenic mutation of LRRK2...
  2. pmc Cardiolipin externalization to the outer mitochondrial membrane acts as an elimination signal for mitophagy in neuronal cells
    Charleen T Chu
    Department of Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    Nat Cell Biol 15:1197-205. 2013
    ..These data indicate that redistribution of cardiolipin serves as an 'eat-me' signal for the elimination of damaged mitochondria from neuronal cells. ..
  3. pmc Impaired mitochondrial biogenesis contributes to depletion of functional mitochondria in chronic MPP+ toxicity: dual roles for ERK1/2
    J H Zhu
    Department of Pathology, Division of Neuropathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    Cell Death Dis 3:e312. 2012
    ..These data indicate a key, limiting role for mitochondrial biogenesis in determining the outcome of injuries associated with elevated mitophagy...
  4. pmc Diversity in the regulation of autophagy and mitophagy: lessons from Parkinson's disease
    Charleen T Chu
    Division of Neuropathology, Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    Parkinsons Dis 2011:789431. 2011
    ....
  5. pmc Location, location, location: altered transcription factor trafficking in neurodegeneration
    Charleen T Chu
    Department of Pathology, Division of Neuropathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    J Neuropathol Exp Neurol 66:873-83. 2007
    ..Studying the pathophysiologic mechanisms regulating transcription factor localization should facilitate strategies to bypass deficits and restore adaptive neuroprotective transcriptional responses...
  6. pmc Beclin 1-independent pathway of damage-induced mitophagy and autophagic stress: implications for neurodegeneration and cell death
    Charleen T Chu
    Department of Pathology, Center for Neuroscience, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Autophagy 3:663-6. 2007
    ....
  7. pmc Tickled PINK1: mitochondrial homeostasis and autophagy in recessive Parkinsonism
    Charleen T Chu
    Department of Pathology Division of Neuropathology, Center for Neuroscience and McGowan Institute for Regenerative Medicine, University of Pittsburgh School of Medicine, 200 Lothrop St, Pittsburgh, PA, USA
    Biochim Biophys Acta 1802:20-8. 2010
    ....
  8. ncbi request reprint Reactive oxygen/nitrogen species at the fulcrum of life-death decisions: a commentary on "peroxynitrite transforms nerve growth factor into an apoptotic factor for motor neurons"
    Charleen T Chu
    Department of Pathology, University of Pittsburgh, Pittsburgh, PA 15213, USA
    Free Radic Biol Med 41:1629-31. 2006
  9. pmc A pivotal role for PINK1 and autophagy in mitochondrial quality control: implications for Parkinson disease
    Charleen T Chu
    Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
    Hum Mol Genet 19:R28-37. 2010
    ....
  10. pmc Autophagic stress in neuronal injury and disease
    Charleen T Chu
    Department of Pathology Division of Neuropathology, Department of Ophthalmology, Center for Neuroscience CNUP, Pittsburgh Institute for Neurodegenerative Diseases PIND, University of Pittsburgh School of Medicine, Pennsylvania, USA
    J Neuropathol Exp Neurol 65:423-32. 2006
    ..The concept of autophagic stress may reconcile apparently conflicting roles of autophagy-related processes in adaptive, homeostatic responses and in pathways of neurodegeneration and cell death...
  11. pmc Eaten alive: autophagy and neuronal cell death after hypoxia-ischemia
    Charleen T Chu
    Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    Am J Pathol 172:284-7. 2008
  12. pmc Apoptosis inducing factor mediates caspase-independent 1-methyl-4-phenylpyridinium toxicity in dopaminergic cells
    Charleen T Chu
    Department of Pathology, Division of Neuropathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
    J Neurochem 94:1685-95. 2005
    ..Studies of primary mouse midbrain cultures further supported a role for AIF in caspase-independent cell death in MPP+-treated dopaminergic neurons...
  13. pmc Functional repression of cAMP response element in 6-hydroxydopamine-treated neuronal cells
    Elisabeth M Chalovich
    Department of Pathology, Division of Neuropathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    J Biol Chem 281:17870-81. 2006
    ....
  14. pmc Regulation of autophagy by extracellular signal-regulated protein kinases during 1-methyl-4-phenylpyridinium-induced cell death
    Jian Hui Zhu
    Department of Pathology Division of Neuropathology, Pittsburgh Institute for Neurodegenerative Diseases, Center for Biologic Imaging, Pittsburgh, PA, USA
    Am J Pathol 170:75-86. 2007
    ..Moreover, pathological stimulation of beclin 1-independent autophagy is associated with neuronal cell death...
  15. pmc Peroxidase mechanism of lipid-dependent cross-linking of synuclein with cytochrome C: protection against apoptosis versus delayed oxidative stress in Parkinson disease
    Hulya Bayir
    Center for Free Radical and Antioxidant Health, Pittsburgh Institute for Neurodegenerative Diseases, University of Pittsburgh, Pittsburgh, Pennsylvania 15219 3130, USA
    J Biol Chem 284:15951-69. 2009
    ..This immediate protective function of Syn is associated with the formation of the peroxidase complex representing a source of oxidative stress and postponed damage...
  16. pmc Autophagy, mitochondria and cell death in lysosomal storage diseases
    Kirill Kiselyov
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    Autophagy 3:259-62. 2007
    ..The resulting accumulation of dysfunctional mitochondria showing impaired Ca2+ buffering increases the vulnerability of the cells to pro-apoptotic signals...
  17. pmc Loss of PINK1 function promotes mitophagy through effects on oxidative stress and mitochondrial fission
    Ruben K Dagda
    Department of Pathology and Center for Neuroscience, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    J Biol Chem 284:13843-55. 2009
    ..Furthermore, PINK1 and Parkin may cooperate through different mechanisms to maintain mitochondrial homeostasis...
  18. pmc Mitochondrial quality control: insights on how Parkinson's disease related genes PINK1, parkin, and Omi/HtrA2 interact to maintain mitochondrial homeostasis
    Ruben K Dagda
    Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    J Bioenerg Biomembr 41:473-9. 2009
    ..We propose that PINK1, Omi/HtrA2 and parkin participate at different levels in mitochondrial quality control, converging through some overlapping and some distinct steps to maintain a common phenotype of healthy mitochondrial networks...
  19. pmc Role of reactive oxygen species in extracellular signal-regulated protein kinase phosphorylation and 6-hydroxydopamine cytotoxicity
    Scott M Kulich
    Department of Pathology, Division of Neuropathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    J Biosci 28:83-9. 2003
    ..This correlation between antioxidant protection and inhibition of 6-OHDA-induced sustained ERK phosphorylation suggests that redox regulation of ERK signalling cascades may contribute to neuronal toxicity...
  20. pmc A novel transferrin/TfR2-mediated mitochondrial iron transport system is disrupted in Parkinson's disease
    Pier Giorgio Mastroberardino
    Department of Neurology, University of Pittsburgh, 3501 Fifth Avenue, Pittsburgh, PA 15260, USA
    Neurobiol Dis 34:417-31. 2009
    ..Thus, we have discovered a novel mitochondrial iron transport system that goes awry in PD, and which may provide a new target for therapeutic intervention...
  21. pmc Oxidative neuronal injury. The dark side of ERK1/2
    Charleen T Chu
    Department of Pathology, Division of Neuropathology, University of Pittsburgh School of Medicine, Room A 516 UPMC Presbyterian, 200 Lothrop Street, Pittsburgh, PA 15213, USA
    Eur J Biochem 271:2060-6. 2004
    ....
  22. ncbi request reprint Mitochondrial dysfunction in Parkinson's disease
    Jianhui Zhu
    Department of Pathology Division of Neuropathology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
    J Alzheimers Dis 20:S325-34. 2010
    ....
  23. pmc Mitochondrial autophagy as a compensatory response to PINK1 deficiency
    Salvatore J Cherra
    Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
    Autophagy 5:1213-4. 2009
    ..These data offer additional insights that broaden the spectrum of potential interactions between PINK1 and parkin with respect to the regulation of mitochondrial homeostasis and mitophagy...
  24. pmc Mitochondrially localized ERK2 regulates mitophagy and autophagic cell stress: implications for Parkinson's disease
    Ruben K Dagda
    Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    Autophagy 4:770-82. 2008
    ..These data indicate that mitochondrial localization of ERK2 activity is sufficient to recapitulate the effects of 6-OHDA on mitophagy and autophagic cell death...
  25. pmc Expression of Nrf2 in neurodegenerative diseases
    Chenere P Ramsey
    Department of Pathology, University of Pennsylvania, School of Dental Medicine, Philadelphia, Pennsylvania, USA
    J Neuropathol Exp Neurol 66:75-85. 2007
    ..In PD, nuclear localization of Nrf2 is strongly induced, but this response may be insufficient to protect neurons from degeneration...
  26. pmc Localization of phosphorylated ERK/MAP kinases to mitochondria and autophagosomes in Lewy body diseases
    Jian Hui Zhu
    Department of Pathology, Division of Neuropathology, University of Pittsburgh, PA, USA
    Brain Pathol 13:473-81. 2003
    ..As mitochondrial pathology may play a pivotal role in Parkinson and other related neurodegenerative diseases, these studies suggest a potential interaction between dysfunctional mitochondria, autophagy, and ERK signaling pathways...
  27. ncbi request reprint Autophagy is increased in mice after traumatic brain injury and is detectable in human brain after trauma and critical illness
    Robert S B Clark
    Department of Critical Care Medicine, The Safar Center for Resuscitation Research and the Brain Trauma Research Center, Pittsburgh, Pennsylvania 15260, USA
    Autophagy 4:88-90. 2008
    ..Taken together, we show that autophagy occurs after both experimental and clinical TBI, and that oxidative stress contributes to overall neuropathology after TBI in mice, at least in part by initiating or influencing autophagy...
  28. ncbi request reprint Mitochondrial aberrations in mucolipidosis Type IV
    John J Jennings
    Department of Biological Science, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    J Biol Chem 281:39041-50. 2006
    ..Deficient Ca2+ homeostasis may represent a common mechanism of degenerative cell death in several lysosomal storage diseases...
  29. pmc Regulation of the autophagy protein LC3 by phosphorylation
    Salvatore J Cherra
    Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    J Cell Biol 190:533-9. 2010
    ..These data demonstrate a role for phosphorylation in regulating LC3 activity...
  30. ncbi request reprint Kinase signaling cascades in the mitochondrion: a matter of life or death
    Craig Horbinski
    Division of Neuropathology, Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    Free Radic Biol Med 38:2-11. 2005
    ....
  31. pmc Cytoplasmic aggregates of phosphorylated extracellular signal-regulated protein kinases in Lewy body diseases
    Jian Hui Zhu
    Department of Pathology, University of Pittsburgh School of Medicine, 200 Lothrop Street, Pittsburgh, PA 15213, USA
    Am J Pathol 161:2087-98. 2002
    ..Moreover, granular cytoplasmic P-ERK was associated with alterations in the distribution of downstream targets such as P-RSK1, but not of P-Elk-1, suggesting functional diversion of ERK-signaling pathways in Lewy body diseases...
  32. pmc 6-Hydroxydopamine induces mitochondrial ERK activation
    Scott M Kulich
    Department of Pathology, VA Pittsburgh Healthcare System, Pittsburgh, PA 15240, USA
    Free Radic Biol Med 43:372-83. 2007
    ....
  33. pmc Mitochondrial kinases in Parkinson's disease: converging insights from neurotoxin and genetic models
    Ruben K Dagda
    Dept of Pathology, University of Pittsburgh, Pittsburgh, PA 15261, United States of America
    Mitochondrion 9:289-98. 2009
    ..Converging mechanisms from different model systems support the concept of common pathways in parkinsonian neurodegeneration that may be amenable to future therapeutic interventions...
  34. ncbi request reprint Autophagy is increased after traumatic brain injury in mice and is partially inhibited by the antioxidant gamma-glutamylcysteinyl ethyl ester
    Yichen Lai
    Department of Critical Care Medicine, The Safar Center for Resuscitation Research and the Brain Trauma Research Center, University of Pittsburgh, Pittsburgh, PA 15260, USA
    J Cereb Blood Flow Metab 28:540-50. 2008
    ..Taken together, these data show that autophagy occurs after experimental TBI, and that oxidative stress contributes to overall neuropathology, in part by initiating or influencing autophagy...
  35. pmc Autophagy in neurite injury and neurodegeneration: in vitro and in vivo models
    Charleen T Chu
    Department of Pathology, Division of Neuropathology, University of Pittsburgh School of Medicine and Center for Neuroscience, Pittsburgh, Pennsylvania, USA
    Methods Enzymol 453:217-49. 2009
    ..Future challenges include application of automated computer-assisted image analysis to the axodendritic tree of individual neurons and improving methods for measuring neuronal autophagic flux...
  36. pmc Starving neurons show sex difference in autophagy
    Lina Du
    Department of Critical Care Medicine, Safar Center for Resuscitation Research, University of Pittsburgh School of Medicine, and Children s Hospital of Pittsburgh, Pittsburgh, PA 15260, USA
    J Biol Chem 284:2383-96. 2009
    ..Thus, during starvation, neurons from males more readily undergo autophagy and die, whereas neurons from females mobilize fatty acids, accumulate triglycerides, form lipid droplets, and survive longer...
  37. pmc The p75 neurotrophin receptor can induce autophagy and death of cerebellar Purkinje neurons
    Maria L Florez-McClure
    Department of Pharmacology, University of Colorado Health Sciences Center, and Denver Veterans Affairs Medical Center, Denver, Colorado 80262, USA
    J Neurosci 24:4498-509. 2004
    ..The autophagic morphology resembles that found in neurodegenerative disorders, suggesting a potential role for this pathway in neurological disease...
  38. pmc Hippocampal long-term potentiation, memory, and longevity in mice that overexpress mitochondrial superoxide dismutase
    Daoying Hu
    Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX 77030, USA
    Neurobiol Learn Mem 87:372-84. 2007
    ..These findings increase our knowledge of the role of mitochondrial superoxide in physiological and pathological processes in the brain...
  39. pmc Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes
    Daniel J Klionsky
    Life Sciences Institute, University of Michigan, Ann Arbor, Michigan 48109 2216, USA
    Autophagy 4:151-75. 2008
    ..In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to verify an autophagic response...

Research Grants11

  1. THE PARKINSONIAN 6 HYDROXYDOPAMINE MODEL
    Charleen Chu; Fiscal Year: 2001
    ....
  2. Regulation of autophagy in dopaminergic cell death
    Charleen T Chu; Fiscal Year: 2010
    ....
  3. Regulation of autophagy in dopaminergic cell death
    Charleen Chu; Fiscal Year: 2009
    ....
  4. Regulation of PTEN-induced kinase 1 (PINK 1)
    Charleen Chu; Fiscal Year: 2007
    ..In addition, a group of immunochemical and molecular reagents will have been created that can facilitate the work of other investigators studying mechanisms of Parkinson's and related diseases. ..
  5. Training in proteomics of novel kinase substrates for neurodegeneration
    Charleen Chu; Fiscal Year: 2007
    ....
  6. Regulation of autophagy in dopaminergic cell death
    Charleen Chu; Fiscal Year: 2007
    ....
  7. THE PARKINSONIAN 6 HYDROXYDOPAMINE MODEL
    Charleen Chu; Fiscal Year: 2004
    ....
  8. THE PARKINSONIAN 6 HYDROXYDOPAMINE MODEL
    Charleen Chu; Fiscal Year: 2003
    ....
  9. THE PARKINSONIAN 6 HYDROXYDOPAMINE MODEL
    Charleen Chu; Fiscal Year: 2002
    ....
  10. Regulation of autophagy in dopaminergic cell death
    Charleen Chu; Fiscal Year: 2009
    ....