Karen L Christman

Summary

Affiliation: University of California
Country: USA

Publications

  1. pmc Increased infarct wall thickness by a bio-inert material is insufficient to prevent negative left ventricular remodeling after myocardial infarction
    Aboli A Rane
    Department of Bioengineering, University of California San Diego, La Jolla, California, United States of America
    PLoS ONE 6:e21571. 2011
  2. pmc Injectable materials for the treatment of myocardial infarction and heart failure: the promise of decellularized matrices
    Jennifer M Singelyn
    Department of Bioengineering, University of California, 9500 Gilman Dr, MC 0412, La Jolla, San Diego, CA 92093, USA
    J Cardiovasc Transl Res 3:478-86. 2010
  3. doi request reprint Surface patterning for generating defined nanoscale matrices
    Karen L Christman
    Department of Bioengineering, University of California, San Diego, CA, USA
    Methods Mol Biol 660:255-63. 2010
  4. pmc A co-culture device with a tunable stiffness to understand combinatorial cell-cell and cell-matrix interactions
    Nikhil Rao
    Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA
    Integr Biol (Camb) 5:1344-54. 2013
  5. pmc Injectable extracellular matrix derived hydrogel provides a platform for enhanced retention and delivery of a heparin-binding growth factor
    Sonya B Seif-Naraghi
    University of California, San Diego, La Jolla, CA, USA
    Acta Biomater 8:3695-703. 2012
  6. pmc Modulation of material properties of a decellularized myocardial matrix scaffold
    Jennifer M Singelyn
    Department of Bioengineering, University of California, San Diego, La Jolla, California 92093, USA
    Macromol Biosci 11:731-8. 2011
  7. pmc Catheter-deliverable hydrogel derived from decellularized ventricular extracellular matrix increases endogenous cardiomyocytes and preserves cardiac function post-myocardial infarction
    Jennifer M Singelyn
    Department of Bioengineering, University of California, San Diego, La Jolla, California, USA
    J Am Coll Cardiol 59:751-63. 2012
  8. ncbi request reprint Injectable myocardial matrix as a scaffold for myocardial tissue engineering
    Jennifer M Singelyn
    Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA
    Conf Proc IEEE Eng Med Biol Soc 2009:2406-8. 2009
  9. pmc Tailoring material properties of a nanofibrous extracellular matrix derived hydrogel
    Todd D Johnson
    Department of Bioengineering, University of California, San Diego 9500 Gilman Drive MC 0412, La Jolla, CA 92093 0412, USA
    Nanotechnology 22:494015. 2011
  10. pmc Positioning multiple proteins at the nanoscale with electron beam cross-linked functional polymers
    Karen L Christman
    Department of Chemistry and Biochemistry, University of California, Los Angeles, 607 Charles E Young Drive East, Los Angeles, California 90095 1569, USA
    J Am Chem Soc 131:521-7. 2009

Research Grants

Collaborators

Detail Information

Publications33

  1. pmc Increased infarct wall thickness by a bio-inert material is insufficient to prevent negative left ventricular remodeling after myocardial infarction
    Aboli A Rane
    Department of Bioengineering, University of California San Diego, La Jolla, California, United States of America
    PLoS ONE 6:e21571. 2011
    ....
  2. pmc Injectable materials for the treatment of myocardial infarction and heart failure: the promise of decellularized matrices
    Jennifer M Singelyn
    Department of Bioengineering, University of California, 9500 Gilman Dr, MC 0412, La Jolla, San Diego, CA 92093, USA
    J Cardiovasc Transl Res 3:478-86. 2010
    ..This review will focus on the requirements of an ideal scaffold for catheter-based delivery as well as highlight the promise of decellularized matrices as injectable materials for cardiac repair...
  3. doi request reprint Surface patterning for generating defined nanoscale matrices
    Karen L Christman
    Department of Bioengineering, University of California, San Diego, CA, USA
    Methods Mol Biol 660:255-63. 2010
    ..We have developed a highly flexible nanopatterning technique, employing protein and peptide reactive polymers and electron beam lithography, which can be utilized for studying matrix effects on stem cell renewal and differentiation...
  4. pmc A co-culture device with a tunable stiffness to understand combinatorial cell-cell and cell-matrix interactions
    Nikhil Rao
    Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA
    Integr Biol (Camb) 5:1344-54. 2013
    ..As this example highlights, this technology better controls the in vitro microenvironment, allowing the user to develop a more thorough understanding of the combined effects of cell-cell and cell-matrix interactions. ..
  5. pmc Injectable extracellular matrix derived hydrogel provides a platform for enhanced retention and delivery of a heparin-binding growth factor
    Sonya B Seif-Naraghi
    University of California, San Diego, La Jolla, CA, USA
    Acta Biomater 8:3695-703. 2012
    ..Thus, the sulfated GAG content of the decellularized ECM hydrogel provides a platform for incorporation of heparin-binding growth factors for prolonged retention and delivery...
  6. pmc Modulation of material properties of a decellularized myocardial matrix scaffold
    Jennifer M Singelyn
    Department of Bioengineering, University of California, San Diego, La Jolla, California 92093, USA
    Macromol Biosci 11:731-8. 2011
    ..These results demonstrate that the material properties of myocardial matrix can be tuned via cross-linking, while maintaining appropriate viscosity for catheter injectability...
  7. pmc Catheter-deliverable hydrogel derived from decellularized ventricular extracellular matrix increases endogenous cardiomyocytes and preserves cardiac function post-myocardial infarction
    Jennifer M Singelyn
    Department of Bioengineering, University of California, San Diego, La Jolla, California, USA
    J Am Coll Cardiol 59:751-63. 2012
    ..This study evaluated the use of an injectable hydrogel derived from ventricular extracellular matrix (ECM) for treating myocardial infarction (MI) and its ability to be delivered percutaneously...
  8. ncbi request reprint Injectable myocardial matrix as a scaffold for myocardial tissue engineering
    Jennifer M Singelyn
    Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA
    Conf Proc IEEE Eng Med Biol Soc 2009:2406-8. 2009
    ..We have also demonstrated that this material may be delivered minimally invasively through a catheter...
  9. pmc Tailoring material properties of a nanofibrous extracellular matrix derived hydrogel
    Todd D Johnson
    Department of Bioengineering, University of California, San Diego 9500 Gilman Drive MC 0412, La Jolla, CA 92093 0412, USA
    Nanotechnology 22:494015. 2011
    ..These results demonstrate how the material properties of ECM hydrogels could be tailored for future in vitro and in vivo applications...
  10. pmc Positioning multiple proteins at the nanoscale with electron beam cross-linked functional polymers
    Karen L Christman
    Department of Chemistry and Biochemistry, University of California, Los Angeles, 607 Charles E Young Drive East, Los Angeles, California 90095 1569, USA
    J Am Chem Soc 131:521-7. 2009
    ..Moreover, multilayer PEG fabrication produced micron- and nanometer-sized patterns of one functional group on top of the other. Precise three-dimensional arrangements of different proteins were then realized...
  11. pmc Safety and efficacy of an injectable extracellular matrix hydrogel for treating myocardial infarction
    Sonya B Seif-Naraghi
    University of California, San Diego, La Jolla, CA 92093, USA
    Sci Transl Med 5:173ra25. 2013
    ..This work therefore provides a strong platform to move forward in clinical studies with this cardiac-specific biomaterial that can be delivered by catheter...
  12. doi request reprint Injectable hydrogel therapies and their delivery strategies for treating myocardial infarction
    Todd D Johnson
    University of California San Diego, Sanford Consortium of Regenerative Medicine, Department of Bioengineering, 2880 Torrey Pines Scenic Drive, La Jolla, CA 92037, USA
    Expert Opin Drug Deliv 10:59-72. 2013
    ..These injectable biomaterials can either be synthetic or naturally derived, and have the potential to be delivered minimally invasively...
  13. pmc Protein nanopatterns by oxime bond formation
    Karen L Christman
    Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California 90095, USA
    Langmuir 27:1415-8. 2011
    ..Protein immobilization was confirmed by fluorescence microscopy. Control studies on protected surfaces and using proteins presaturated with O-methoxyamine indicated that attachment occurred via oxime bond formation...
  14. pmc Tunable protein release from acetalated dextran microparticles: a platform for delivery of protein therapeutics to the heart post-MI
    Sophia Suarez
    Department of Bioengineering and Sanford Consortium of Regenerative Medicine and Skaggs School of Pharmacy and Pharmaceutical Sciences and KACST UCSD Center of Excellence in Nanomedicine, University of California, San Diego, La Jolla, California, United States
    Biomacromolecules 14:3927-35. 2013
    ..The release was shown in low pH environments, similar to what is found in an infarcted heart. bFGF maintained activity after release from the microparticles. Finally, biocompatibility of the microparticles was assessed. ..
  15. pmc Design and characterization of an injectable pericardial matrix gel: a potentially autologous scaffold for cardiac tissue engineering
    Sonya B Seif-Naraghi
    Department of Bioengineering, University of California, San Diego, La Jolla, California 92093 0412, USA
    Tissue Eng Part A 16:2017-27. 2010
    ..With this work, the pericardium is identified as a novel source for an autologous scaffold for treating myocardial infarction...
  16. pmc Naturally derived myocardial matrix as an injectable scaffold for cardiac tissue engineering
    Jennifer M Singelyn
    Department of Bioengineering, University of California, San Diego, 9500 Gilman Dr, La Jolla, CA 92093 0412, USA
    Biomaterials 30:5409-16. 2009
    ..Thus, we have demonstrated the initial feasibility and potential of a naturally derived myocardial matrix as an injectable scaffold for cardiac tissue engineering...
  17. pmc Antibacterial and cell-adhesive polypeptide and poly(ethylene glycol) hydrogel as a potential scaffold for wound healing
    Airong Song
    Bioengineering Department, University of California, San Diego, CA 92093, USA
    Acta Biomater 8:41-50. 2012
    ..Thus, we have developed a novel, cell-adhesive hydrogel with inherent antibacterial activity as a potential scaffold for cutaneous wound healing...
  18. pmc Injectable skeletal muscle matrix hydrogel promotes neovascularization and muscle cell infiltration in a hindlimb ischemia model
    Jessica A DeQuach
    Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA
    Eur Cell Mater 23:400-12; discussion 412. 2012
    ..Our results indicate that this tissue-specific injectable hydrogel may be a potential therapy for treating ischemia related to PAD, as well as have potential beneficial effects on restoring muscle mass that is typically lost in CLI...
  19. pmc Stimulation of adipogenesis of adult adipose-derived stem cells using substrates that mimic the stiffness of adipose tissue
    D Adam Young
    Department of Bioengineering, University of California, San Diego, Sanford Consortium for Regenerative Medicine, 2880 Torrey Pines Scenic Dr, La Jolla, CA 92037, USA
    Biomaterials 34:8581-8. 2013
    ..Together these data imply that as with other lineages, mechanical cues are capable of regulating adipogenesis in ASCs. ..
  20. pmc Nanoscale growth factor patterns by immobilization on a heparin-mimicking polymer
    Karen L Christman
    Department of Chemistry and Biochemistry, University of California, Los Angeles, 607 Charles E Young Drive East, Los Angeles, California 90095 1569, USA
    J Am Chem Soc 130:16585-91. 2008
    ..Fluorescence microscopy confirmed that bFGF and VEGF were subsequently immobilized to the polymer micro- and nanopatterns...
  21. pmc Fibroblasts influence muscle progenitor differentiation and alignment in contact independent and dependent manners in organized co-culture devices
    Nikhil Rao
    Department of Bioengineering, University of California, San Diego 2880 Torrey Pines Scenic Drive, La Jolla, CA 92037, USA
    Biomed Microdevices 15:161-9. 2013
    ..These findings could have direct implications on cell therapies for repairing skeletal muscle, which have only utilized skeletal myoblasts or stem cell populations alone...
  22. pmc Decellularized porcine brain matrix for cell culture and tissue engineering scaffolds
    Jessica A DeQuach
    Department of Bioengineering, University of California, San Diego, La Jolla, California 92092, USA
    Tissue Eng Part A 17:2583-92. 2011
    ..Thus, our work demonstrates the ability to use decellularized brain ECM for cell culture and tissue engineering applications...
  23. pmc Patient-to-patient variability in autologous pericardial matrix scaffolds for cardiac repair
    Sonya B Seif-Naraghi
    University of California, San Diego, La Jolla, CA, USA
    J Cardiovasc Transl Res 4:545-56. 2011
    ....
  24. doi request reprint Biomaterials for the treatment of myocardial infarction: a 5-year update
    Aboli A Rane
    Department of Bioengineering, University of California San Diego, La Jolla, USA
    J Am Coll Cardiol 58:2615-29. 2011
    ..This review focuses on the progress made in the field of cardiac biomaterial treatments for MI over the last 5 years...
  25. doi request reprint Restoration of left ventricular geometry and improvement of left ventricular function in a rodent model of chronic ischemic cardiomyopathy
    Jiashing Yu
    University of California Berkeley and University of California San Francisco Joint Bioengineering Graduate Group, Berkeley San Francisco, CA, USA
    J Thorac Cardiovasc Surg 137:180-7. 2009
    ..Various approaches to myocardial reconstruction have been developed for the treatment of congestive heart failure resulting from ischemic cardiomyopathy...
  26. pmc In vivo response to dynamic hyaluronic acid hydrogels
    Jennifer L Young
    Department of Bioengineering, University of California, San Diego, CA 92093, USA
    Acta Biomater 9:7151-7. 2013
    ..Altogether these data suggest that the HA-SH hydrogel responds appropriately in a less vascularized niche and stiffens as had been demonstrated in vitro, but in more vascularized tissues, in vivo applicability appears limited...
  27. pmc Human cardiomyogenesis and the need for systems biology analysis
    D Adam Young
    Department of Bioengineering, University of California, San Diego, CA, USA
    Wiley Interdiscip Rev Syst Biol Med 3:666-80. 2011
    ..Investigating this phenomenon from a systems biology perspective could unveil the abstruse mechanisms controlling cardiomyogenesis that would otherwise require extensive in vitro testing...
  28. pmc Simple and high yielding method for preparing tissue specific extracellular matrix coatings for cell culture
    Jessica A DeQuach
    Department of Bioengineering, University of California San Diego, La Jolla, California, United States of America
    PLoS ONE 5:e13039. 2010
    ..Despite the complex nature of the ECM, in vitro cell-based studies traditionally assess cell behavior on single ECM component substrates, which do not adequately mimic the in vivo extracellular milieu...
  29. pmc Injectable hydrogel scaffold from decellularized human lipoaspirate
    D Adam Young
    Department of Bioengineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0412, USA
    Acta Biomater 7:1040-9. 2011
    ..The development of an injectable hydrogel from human lipoaspirate represents a minimally invasive option for adipose tissue engineering in terms of both the collection of source material and delivery of the scaffold...
  30. ncbi request reprint Biomaterials for the treatment of myocardial infarction
    Karen L Christman
    University of California Berkeley, Berkeley, California, USA
    J Am Coll Cardiol 48:907-13. 2006
    ..This review gives an overview of the current progress in the growing field of biomaterials for the treatment of MI...
  31. pmc Myocardial matrix-polyethylene glycol hybrid hydrogels for tissue engineering
    Gregory N Grover
    Department of Bioengineering, Sanford Consortium for Regenerative Medicine, University of California, San Diego, 92093, USA
    Nanotechnology 25:014011. 2014
    ..This work demonstrates that PEG incorporation into ECM-based hydrogels can expand material properties, thereby opening up new possibilities for in vitro and in vivo applications...
  32. doi request reprint Treating the leading killer
    Karen L Christman
    Department of Bioengineering, University of California San Diego, La Jolla, CA 92093, USA
    Sci Transl Med 4:146fs26. 2012
    ..Injection of a growth factor in a self-assembling nanofiber scaffold has the potential to create a suitable microenvironment and recruit endogenous cells for cardiac repair (Lin et al., this issue)...
  33. ncbi request reprint Submicron streptavidin patterns for protein assembly
    Karen L Christman
    Department of Chemistry and Biochemistry, Department of Microbiology, Immunology, and Molecular Genetics, California NanoSystems Institute, University of California, Los Angeles, Los Angeles, California 90095, USA
    Langmuir 22:7444-50. 2006
    ..AFM demonstrated that 500 nm protein features were achieved. Because of the abundance of biotinylated proteins, this methodology provides a platform for protein immobilization and assembly for various applications in biotechnology...

Research Grants1

  1. Selective patterning of biomolecules into protein arrays
    Karen Christman; Fiscal Year: 2006
    ..This technology may also be applied to the detection and diagnosis of a variety of other diseases. ..