Research Topics
| Daniel ChiuSummaryAffiliation: University of Washington Country: USA Publications
Research Grants
| Collaborators
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Detail Information
Publications
A versatile method for generating semiconducting polymer dot nanocompositesWei Sun
Department of Chemistry, University of Washington, Seattle, Washington 98195, USA
Nanoscale 4:7246-9. 2012..The method we present here should be generalizable to the formation of other Pdot nanocomposites for creating the next generation of multi-functional Pdot probes...- Digital LAMP in a sample self-digitization (SD) chipAlexander Gansen
Department of Chemistry, University of Washington, Seattle, USA
Lab Chip 12:2247-54. 2012..We anticipate that the simplicity and robustness of our SD chip make it attractive as an inexpensive and easy-to-operate device for DNA amplification, for example in point-of-care settings... 
Generation of functionalized and robust semiconducting polymer dots with polyelectrolytesYuhui Jin
Department of Chemistry, University of Washington, Seattle, WA, USA
Chem Commun (Camb) 48:3161-3. 2012..We conjugated the polyelectrolyte-coated Pdots with streptavidin to demonstrate their application in specific cell labeling...
A rapid and economical method for profiling feature heights during microfabricationGloria S Yen
Department of Chemistry, University of Washington, Seattle, WA 98195 1700, USA
Lab Chip 11:974-7. 2011..This technique is based on white-light interferometry, which offers accuracy on the submicrometre scale...- Interfacing droplet microfluidics with chemical separation for cellular analysisDaniel T Chiu
Department of Chemistry, University of Washington, Seattle, WA 98195 1700, USA
Anal Bioanal Chem 397:3179-83. 2010..This article reviews the current state-of-the-art in this new area and provides an outlook on the role of this new technique in cellular analysis... - Cellular manipulations in microvorticesDaniel T Chiu
Department of Chemistry, University of Washington, Seattle, WA 98195-1700, USA
Anal Bioanal Chem 387:17-20. 2007 - A compact and highly fluorescent orange-emitting polymer dot for specific subcellular imagingFangmao Ye
Department of Chemistry, University of Washington, Seattle, WA, USA
Chem Commun (Camb) 48:1778-80. 2012..The small particle size, high brightness, and the ability to highly specifically target subcellular structures make the CN-PPV dots promising probes for biological imaging and bioanalytical applications... - Droplets for ultrasmall-volume analysisDaniel T Chiu
University of Washington Seattle, USA
Anal Chem 81:5111-8. 2009..To listen to a podcast about this feature, please go to the Analytical Chemistry Web site at pubs.acs.org/ancham.)... - Chemistry and biology in femtoliter and picoliter volume dropletsDaniel T Chiu
Department of Chemistry, University of Washington, Box 351700, Seattle, Washington 98195 1700, USA
Acc Chem Res 42:649-58. 2009..With progress in these areas, we hope to cement our technique as a new tool for studying single cells and organelles with unprecedented molecular detail... - Disposable microfluidic substrates: transitioning from the research laboratory into the clinicJason S Kuo
Department of Chemistry, University of Washington, Seattle, WA 98195 1700, USA
Lab Chip 11:2656-65. 2011..We also review the recent trend in microfluidic devices constructed from a hybrid of substrates that takes advantage of each material's attributes... - Controlling mass transport in microfluidic devicesJason S Kuo
Department of Chemistry, University of Washington, Seattle, Washington 98195 1700, USA
Annu Rev Anal Chem (Palo Alto Calif) 4:275-96. 2011..We also highlight several in vitro biological studies in the areas of organ engineering, cancer, and blood coagulation that have benefited from accurate control of mass transfer... - Systematic investigation of droplet generation at T-junctionsThomas Schneider
Department of Chemistry, University of Washington, Seattle, WA 98195 1700, USA
Lab Chip 11:2055-9. 2011..Our experimental results, together with fluid dynamic simulations, allowed us to highlight the importance of physical fluid properties when employing theoretical models... - Copper(II) and iron(II) ion sensing with semiconducting polymer dotsYang Hsiang Chan
Department of Chemistry, University of Washington, Seattle, WA, USA
Chem Commun (Camb) 47:2820-2. 2011.... - Deformability considerations in filtration of biological cellsJason S Kuo
Department of Chemistry, University of Washington, Seattle, WA 98195 1700, USA
Lab Chip 10:837-42. 2010..We describe the underlying biophysics of cellular stress and general solutions to scale up filtration processes for biological cells... - Controlled rotation of biological micro- and nano-particles in microvorticesJ Patrick Shelby
Department of Chemistry, University of Washington, Seattle, WA 98195-1700, USA
Lab Chip 4:168-70. 2004..We demonstrate in such microvortices the orientation control of single DNA molecules, and the axial rotation of biological cells in which the cellular contents were visibly affected by rotation... - Direct manipulation and observation of the rotational motion of single optically trapped microparticles and biological cells in microvorticesJ Patrick Shelby
Department of Chemistry, University of Washington, Seattle, Washington 98195-1700, USA
Anal Chem 76:2492-7. 2004..This technique provides a simple and sensitive method for controlling and monitoring the rotational motion of microparticles in a microfluidic environment... - Calcium-assisted glass-to-glass bonding for fabrication of glass microfluidic devicesPeter B Allen
Department of Chemistry, University of Washington, Box 351700, Seattle, Washington 98195, USA
Anal Chem 80:7153-7. 2008..This technique is compatible with inexpensive unpolished glass and is limited in resolution by the PDMS etch guide used and the intrinsic properties of isotropic etching... - Single-synapse ablation and long-term imaging in live C. elegansPeter B Allen
Department of Chemistry, University of Washington, Box 351700, Seattle, WA 98195 1700, United States
J Neurosci Methods 173:20-6. 2008..elegans to facilitate long-term fluorescence imaging and observation of neuronal development. With this technique, we directly demonstrated the existence of competition between developing synapses in the HSNL motor neuron... - Sizing subcellular organelles and nanoparticles confined within aqueous dropletsJennifer C Gadd
Department of Chemistry, University of Washington, Box 351700, Seattle, Washington 98195 1700, USA
Anal Chem 80:3450-7. 2008.... - Fabrication of thermoset polyester microfluidic devices and embossing masters using rapid prototyped polydimethylsiloxane moldsGina S Fiorini
Department of Chemistry, University of Washington, Seattle, WA, USA
Lab Chip 3:158-63. 2003..Examples of enclosed polyester and PMMA microchannels are presented, and we discuss the electroosmotic properties of both types of channels, which are important for analytical applications such as capillary electrophoresis... - Rapid prototyping of thermoset polyester microfluidic devicesGina S Fiorini
Department of Chemistry, University of Washington, Seattle, Washington 98195-1700, USA
Anal Chem 76:4697-704. 2004.... - Selective encapsulation of single cells and subcellular organelles into picoliter- and femtoliter-volume dropletsMingyan He
Department of Chemistry, University of Washington, Seattle, Washington 98195-1700, USA
Anal Chem 77:1539-44. 2005..This ability to entrap individual selected cells or subcellular organelles should open new possibilities for carrying out single-cell studies and single-organelle measurements... - Disposable microfluidic devices: fabrication, function, and applicationGina S Fiorini
University of Washington, Seattle, WA 98195-1700, USA
Biotechniques 38:429-46. 2005..Finally, a few select biotechnological applications of microfluidics are presented to illustrate both the utility of this technology and its potential for development in the future... - Microfluidic technologies in drug discoveryJohan Pihl
Cellectricon AB, Fabriksgatan 7, SE 412 50 Gothenburg, Sweden
Drug Discov Today 10:1377-83. 2005..This article will focus on the implementation of microfluidic systems and technologies in the process of drug discovery... - Real-time sizing of nanoparticles in microfluidic channels using confocal correlation spectroscopyChristopher L Kuyper
Department of Chemistry, University of Washington, Seattle, Washington 98195-1700, USA
J Am Chem Soc 128:730-1. 2006..With advances in nonfluorescent modes of single-particle and single-molecule detection, we anticipate CCS will find broad use in the real-time measurements of nanoparticles and macromolecules... - Proton permeation into single vesicles occurs via a sequential two-step mechanism and is heterogeneousChristopher L Kuyper
Department of Chemistry, University of Washington, Seattle, Washington 98195-1700, USA
J Am Chem Soc 128:3233-40. 2006.... - Effects of ultrasmall orifices on the electrogeneration of femtoliter-volume aqueous dropletsMingyan He
Department of Chemistry, University of Washington, Seattle, Washington 98195, USA
Langmuir 22:6408-13. 2006.... - Thermoelectric manipulation of aqueous droplets in microfluidic devicesAllyson E Sgro
Department of Chemistry, University of Washington, Seattle, Washington 98195 1700, USA
Anal Chem 79:4845-51. 2007.... - Continuous-flow single-molecule CE with high detection efficiencyPerry G Schiro
Department of Chemistry, University of Washington, Seattle, WA 98195 1700, USA
Electrophoresis 28:2430-8. 2007..We successfully separated single molecules of FITC, FITC-tagged glutamate, and FITC-tagged glycine... - Fabrication improvements for thermoset polyester (TPE) microfluidic devicesGina S Fiorini
Department of Chemistry, University of Washington, Seattle, WA, USA
Lab Chip 7:923-6. 2007..We also describe plasma-bonding of TPE to glass to create hybrid TPE-glass devices. We further present a simple master-pretreatment method to replace our original technique that required the use of specialized equipment... - In-channel atom-transfer radical polymerization of thermoset polyester microfluidic devices for bioanalytical applicationsTao Pan
Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602 5700, USA
Electrophoresis 28:2904-11. 2007..Our results indicate that PEG-grafted TPE microchips have broad potential application in biomolecular analysis... - Alzheimer's disease protein Abeta1-42 does not disrupt isolated synaptic vesiclesPeter B Allen
University of Washington, Department of Chemistry, Box 351700, Seattle, WA 98195, USA
Biochim Biophys Acta 1782:326-34. 2008..From these experiments, we conclude that isolated synaptic vesicles are not disrupted by Abeta1-42... - Parametric investigation on the effect of channel topologies on electrophoretic separationsDavid S W Lim
Department of Chemistry, University of Washington, Seattle, WA 98195-1700, USA
J Chromatogr A 1027:237-44. 2004....
Research Grants
- Profiling Biochemical Contents of Single MitochondriaDaniel Chiu; Fiscal Year: 2009..While our focus here is on the analysis of individual mitochondria, we believe our method can be applied equally well to the chemical analysis of other subcellular organelles and compartments. ..
- Single-Molecule Studies of Synaptic VesiclesDaniel Chiu; Fiscal Year: 2009....
- High-Resolution Analysis of Synaptic ProteinsDaniel Chiu; Fiscal Year: 2009..4. To examine the effects of aberrant synaptic activity on vesicular protein composition. ..
- Single-Molecule Studies of Synaptic VesiclesDaniel T Chiu; Fiscal Year: 2010..abstract_text> ..
- High-Resolution Analysis of Synaptic ProteinsDaniel T Chiu; Fiscal Year: 2010....
- Super-Resolution Imaging with Difference Deconvolution MicroscopyDaniel T Chiu; Fiscal Year: 2010..DDM uses comparable experimental parameters (e.g. similar laser powers) as confocal microscopy, and thus is inherently suited to use with fragile samples, such as live mammalian cells. ..
- Profiling individual synaptic vesicles with nanofluidicsDaniel Chiu; Fiscal Year: 2003....
- Probing single proteins in nanofabricated bioenvironmentDaniel Chiu; Fiscal Year: 2006..Gaining a better understanding of the true in vivo activities of proteins inside cells is critical for developing new drugs that target these proteins for the treatment of devastating diseases. ..
- Profiling Biochemical Contents of Single MitochondriaDaniel Chiu; Fiscal Year: 2007..While our focus here is on the analysis of individual mitochondria, we believe our method can be applied equally well to the chemical analysis of other subcellular organelles and compartments. ..
- Spatially and Temporally Resolved Perturbation of CellsDaniel Chiu; Fiscal Year: 2007..This method will facilitate the detailed study of signaling pathways that underlie disease processes, such as Alzheimer that is a devastating neurological disease associated with aging. ..
- High-Resolution Analysis of Synaptic ProteinsDaniel T Chiu; Fiscal Year: 2010....