Daniel Chiu

Summary

Affiliation: University of Washington
Country: USA

Publications

  1. ncbi request reprint Microfluidics for single-cell genetic analysis
    A M Thompson
    Department of Chemistry, University of Washington, Seattle, WA, USA
    Lab Chip 14:3135-42. 2014
  2. ncbi request reprint Semiconducting polymer dots with monofunctional groups
    Fangmao Ye
    Department of Chemistry, University of Washington, Seattle, Washington 98195, USA
    Chem Commun (Camb) 50:5604-7. 2014
  3. pmc Effects of aliasing on the fidelity of a two dimensional array of foci generated with a kinoform
    D R Burnham
    Department of Chemistry, University of Washington, Seattle, Washington 98195 170, USA
    Opt Express 19:17121-6. 2011
  4. doi request reprint Highly luminescent, fluorinated semiconducting polymer dots for cellular imaging and analysis
    Yong Zhang
    Department of Chemistry, University of Washington, Seattle, Washington, USA
    Chem Commun (Camb) 49:8256-8. 2013
  5. pmc A versatile method for generating semiconducting polymer dot nanocomposites
    Wei Sun
    Department of Chemistry, University of Washington, Seattle, Washington 98195, USA
    Nanoscale 4:7246-9. 2012
  6. pmc Digital LAMP in a sample self-digitization (SD) chip
    Alexander Gansen
    Department of Chemistry, University of Washington, Seattle, USA
    Lab Chip 12:2247-54. 2012
  7. pmc Generation of functionalized and robust semiconducting polymer dots with polyelectrolytes
    Yuhui Jin
    Department of Chemistry, University of Washington, Seattle, WA, USA
    Chem Commun (Camb) 48:3161-3. 2012
  8. ncbi request reprint Interfacing droplet microfluidics with chemical separation for cellular analysis
    Daniel T Chiu
    Department of Chemistry, University of Washington, Seattle, WA 98195 1700, USA
    Anal Bioanal Chem 397:3179-83. 2010
  9. ncbi request reprint A rapid and economical method for profiling feature heights during microfabrication
    Gloria S Yen
    Department of Chemistry, University of Washington, Seattle, WA 98195 1700, USA
    Lab Chip 11:974-7. 2011
  10. ncbi request reprint Droplets for ultrasmall-volume analysis
    Daniel T Chiu
    University of Washington Seattle, USA
    Anal Chem 81:5111-8. 2009

Collaborators

  • Wei Sun
  • Luke P Lee
  • Jason S Kuo
  • Gina S Fiorini
  • Peter B Allen
  • Fangmao Ye
  • Christopher L Kuyper
  • Yang Hsiang Chan
  • J Patrick Shelby
  • Changfeng Wu
  • Yuhui Jin
  • Perry G Schiro
  • Jiangbo Yu
  • Gavin D M Jeffries
  • Thomas Schneider
  • Mingyan He
  • Robert M Lorenz
  • David S W Lim
  • Allyson E Sgro
  • A M Thompson
  • Sarah A Mutch
  • Yu Rong
  • Yong Zhang
  • Alexander Gansen
  • Gloria S Yen
  • D R Burnham
  • Bryant S Fujimoto
  • Jennifer C Gadd
  • Tao Pan
  • J Scott Edgar
  • Johan Pihl
  • Xuanjun Zhang
  • I Che Wu
  • Yue Zhang
  • J P Radich
  • J E Kreutz
  • A L Paguirigan
  • Maria Elena Gallina
  • Alison M Herrick
  • Sarah Hayden
  • Meng Wang
  • Ivan K Dimov
  • Jaylen Vanorden
  • Daniel R Burnham
  • T Schneider
  • Desmond T K Huynh
  • Laiying Ng
  • Yongxi Zhao
  • Richard W Allen
  • Byron E Doepker
  • Kang Shen
  • Daniel L Chao
  • Adam T Woolley
  • Moonbin Yim
  • Kristi L Budzinski
  • Mattias Karlsson

Detail Information

Publications37

  1. ncbi request reprint Microfluidics for single-cell genetic analysis
    A M Thompson
    Department of Chemistry, University of Washington, Seattle, WA, USA
    Lab Chip 14:3135-42. 2014
    ....
  2. ncbi request reprint Semiconducting polymer dots with monofunctional groups
    Fangmao Ye
    Department of Chemistry, University of Washington, Seattle, Washington 98195, USA
    Chem Commun (Camb) 50:5604-7. 2014
    ..This communication describes an approach for preparing monovalent semiconducting polymer dots (mPdots) with a size of 5 nm where each mPdot was composed of precisely a single active functional group. ..
  3. pmc Effects of aliasing on the fidelity of a two dimensional array of foci generated with a kinoform
    D R Burnham
    Department of Chemistry, University of Washington, Seattle, Washington 98195 170, USA
    Opt Express 19:17121-6. 2011
    ..This study provides a reference for applications where it is important to understand how the fidelity and overall quality of the foci array changes as the number of foci increases and as the spacing between foci decreases...
  4. doi request reprint Highly luminescent, fluorinated semiconducting polymer dots for cellular imaging and analysis
    Yong Zhang
    Department of Chemistry, University of Washington, Seattle, Washington, USA
    Chem Commun (Camb) 49:8256-8. 2013
    ..The fluorinated Pdot was eight times brighter in cell-labeling applications than its non-fluorinated counterpart, and was rod shaped rather than spherical. ..
  5. pmc A versatile method for generating semiconducting polymer dot nanocomposites
    Wei Sun
    Department of Chemistry, University of Washington, Seattle, Washington 98195, USA
    Nanoscale 4:7246-9. 2012
    ..The method we present here should be generalizable to the formation of other Pdot nanocomposites for creating the next generation of multi-functional Pdot probes...
  6. pmc Digital LAMP in a sample self-digitization (SD) chip
    Alexander Gansen
    Department of Chemistry, University of Washington, Seattle, USA
    Lab Chip 12:2247-54. 2012
    ..We anticipate that the simplicity and robustness of our SD chip make it attractive as an inexpensive and easy-to-operate device for DNA amplification, for example in point-of-care settings...
  7. pmc Generation of functionalized and robust semiconducting polymer dots with polyelectrolytes
    Yuhui Jin
    Department of Chemistry, University of Washington, Seattle, WA, USA
    Chem Commun (Camb) 48:3161-3. 2012
    ..We conjugated the polyelectrolyte-coated Pdots with streptavidin to demonstrate their application in specific cell labeling...
  8. ncbi request reprint Interfacing droplet microfluidics with chemical separation for cellular analysis
    Daniel T Chiu
    Department of Chemistry, University of Washington, Seattle, WA 98195 1700, USA
    Anal Bioanal Chem 397:3179-83. 2010
    ..This article reviews the current state-of-the-art in this new area and provides an outlook on the role of this new technique in cellular analysis...
  9. ncbi request reprint A rapid and economical method for profiling feature heights during microfabrication
    Gloria S Yen
    Department of Chemistry, University of Washington, Seattle, WA 98195 1700, USA
    Lab Chip 11:974-7. 2011
    ..This technique is based on white-light interferometry, which offers accuracy on the submicrometre scale...
  10. ncbi request reprint Droplets for ultrasmall-volume analysis
    Daniel T Chiu
    University of Washington Seattle, USA
    Anal Chem 81:5111-8. 2009
    ..To listen to a podcast about this feature, please go to the Analytical Chemistry Web site at pubs.acs.org/ancham.)...
  11. ncbi request reprint Cellular manipulations in microvortices
    Daniel T Chiu
    Department of Chemistry, University of Washington, Seattle, WA 98195 1700, USA
    Anal Bioanal Chem 387:17-20. 2007
  12. pmc Chemistry and biology in femtoliter and picoliter volume droplets
    Daniel T Chiu
    Department of Chemistry, University of Washington, Box 351700, Seattle, Washington 98195 1700, USA
    Acc Chem Res 42:649-58. 2009
    ..With progress in these areas, we hope to cement our technique as a new tool for studying single cells and organelles with unprecedented molecular detail...
  13. pmc A compact and highly fluorescent orange-emitting polymer dot for specific subcellular imaging
    Fangmao Ye
    Department of Chemistry, University of Washington, Seattle, WA, USA
    Chem Commun (Camb) 48:1778-80. 2012
    ..The small particle size, high brightness, and the ability to highly specifically target subcellular structures make the CN-PPV dots promising probes for biological imaging and bioanalytical applications...
  14. doi request reprint Disposable microfluidic substrates: transitioning from the research laboratory into the clinic
    Jason S Kuo
    Department of Chemistry, University of Washington, Seattle, WA 98195 1700, USA
    Lab Chip 11:2656-65. 2011
    ..We also review the recent trend in microfluidic devices constructed from a hybrid of substrates that takes advantage of each material's attributes...
  15. doi request reprint Controlling mass transport in microfluidic devices
    Jason S Kuo
    Department of Chemistry, University of Washington, Seattle, Washington 98195 1700, USA
    Annu Rev Anal Chem (Palo Alto Calif) 4:275-96. 2011
    ..We also highlight several in vitro biological studies in the areas of organ engineering, cancer, and blood coagulation that have benefited from accurate control of mass transfer...
  16. ncbi request reprint Systematic investigation of droplet generation at T-junctions
    Thomas Schneider
    Department of Chemistry, University of Washington, Seattle, WA 98195 1700, USA
    Lab Chip 11:2055-9. 2011
    ..Our experimental results, together with fluid dynamic simulations, allowed us to highlight the importance of physical fluid properties when employing theoretical models...
  17. doi request reprint Copper(II) and iron(II) ion sensing with semiconducting polymer dots
    Yang Hsiang Chan
    Department of Chemistry, University of Washington, Seattle, WA, USA
    Chem Commun (Camb) 47:2820-2. 2011
    ....
  18. ncbi request reprint Deformability considerations in filtration of biological cells
    Jason S Kuo
    Department of Chemistry, University of Washington, Seattle, WA 98195 1700, USA
    Lab Chip 10:837-42. 2010
    ..We describe the underlying biophysics of cellular stress and general solutions to scale up filtration processes for biological cells...
  19. ncbi request reprint Controlled rotation of biological micro- and nano-particles in microvortices
    J Patrick Shelby
    Department of Chemistry, University of Washington, Seattle, WA 98195 1700, USA
    Lab Chip 4:168-70. 2004
    ..We demonstrate in such microvortices the orientation control of single DNA molecules, and the axial rotation of biological cells in which the cellular contents were visibly affected by rotation...
  20. ncbi request reprint Direct manipulation and observation of the rotational motion of single optically trapped microparticles and biological cells in microvortices
    J Patrick Shelby
    Department of Chemistry, University of Washington, Seattle, Washington 98195 1700, USA
    Anal Chem 76:2492-7. 2004
    ..This technique provides a simple and sensitive method for controlling and monitoring the rotational motion of microparticles in a microfluidic environment...
  21. doi request reprint Calcium-assisted glass-to-glass bonding for fabrication of glass microfluidic devices
    Peter B Allen
    Department of Chemistry, University of Washington, Box 351700, Seattle, Washington 98195, USA
    Anal Chem 80:7153-7. 2008
    ..This technique is compatible with inexpensive unpolished glass and is limited in resolution by the PDMS etch guide used and the intrinsic properties of isotropic etching...
  22. ncbi request reprint Fabrication of thermoset polyester microfluidic devices and embossing masters using rapid prototyped polydimethylsiloxane molds
    Gina S Fiorini
    Department of Chemistry, University of Washington, Seattle, WA, USA
    Lab Chip 3:158-63. 2003
    ..Examples of enclosed polyester and PMMA microchannels are presented, and we discuss the electroosmotic properties of both types of channels, which are important for analytical applications such as capillary electrophoresis...
  23. ncbi request reprint Rapid prototyping of thermoset polyester microfluidic devices
    Gina S Fiorini
    Department of Chemistry, University of Washington, Seattle, Washington 98195 1700, USA
    Anal Chem 76:4697-704. 2004
    ....
  24. ncbi request reprint Selective encapsulation of single cells and subcellular organelles into picoliter- and femtoliter-volume droplets
    Mingyan He
    Department of Chemistry, University of Washington, Seattle, Washington 98195 1700, USA
    Anal Chem 77:1539-44. 2005
    ..This ability to entrap individual selected cells or subcellular organelles should open new possibilities for carrying out single-cell studies and single-organelle measurements...
  25. ncbi request reprint Disposable microfluidic devices: fabrication, function, and application
    Gina S Fiorini
    University of Washington, Seattle, WA 98195 1700, USA
    Biotechniques 38:429-46. 2005
    ..Finally, a few select biotechnological applications of microfluidics are presented to illustrate both the utility of this technology and its potential for development in the future...
  26. ncbi request reprint Microfluidic technologies in drug discovery
    Johan Pihl
    Cellectricon AB, Fabriksgatan 7, SE 412 50 Gothenburg, Sweden
    Drug Discov Today 10:1377-83. 2005
    ..This article will focus on the implementation of microfluidic systems and technologies in the process of drug discovery...
  27. ncbi request reprint Real-time sizing of nanoparticles in microfluidic channels using confocal correlation spectroscopy
    Christopher L Kuyper
    Department of Chemistry, University of Washington, Seattle, Washington 98195 1700, USA
    J Am Chem Soc 128:730-1. 2006
    ..With advances in nonfluorescent modes of single-particle and single-molecule detection, we anticipate CCS will find broad use in the real-time measurements of nanoparticles and macromolecules...
  28. ncbi request reprint Proton permeation into single vesicles occurs via a sequential two-step mechanism and is heterogeneous
    Christopher L Kuyper
    Department of Chemistry, University of Washington, Seattle, Washington 98195 1700, USA
    J Am Chem Soc 128:3233-40. 2006
    ....
  29. pmc Effects of ultrasmall orifices on the electrogeneration of femtoliter-volume aqueous droplets
    Mingyan He
    Department of Chemistry, University of Washington, Seattle, Washington 98195, USA
    Langmuir 22:6408-13. 2006
    ....
  30. pmc Thermoelectric manipulation of aqueous droplets in microfluidic devices
    Allyson E Sgro
    Department of Chemistry, University of Washington, Seattle, Washington 98195 1700, USA
    Anal Chem 79:4845-51. 2007
    ....
  31. ncbi request reprint Continuous-flow single-molecule CE with high detection efficiency
    Perry G Schiro
    Department of Chemistry, University of Washington, Seattle, WA 98195 1700, USA
    Electrophoresis 28:2430-8. 2007
    ..We successfully separated single molecules of FITC, FITC-tagged glutamate, and FITC-tagged glycine...
  32. ncbi request reprint Fabrication improvements for thermoset polyester (TPE) microfluidic devices
    Gina S Fiorini
    Department of Chemistry, University of Washington, Seattle, WA, USA
    Lab Chip 7:923-6. 2007
    ..We also describe plasma-bonding of TPE to glass to create hybrid TPE-glass devices. We further present a simple master-pretreatment method to replace our original technique that required the use of specialized equipment...
  33. pmc In-channel atom-transfer radical polymerization of thermoset polyester microfluidic devices for bioanalytical applications
    Tao Pan
    Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602 5700, USA
    Electrophoresis 28:2904-11. 2007
    ..Our results indicate that PEG-grafted TPE microchips have broad potential application in biomolecular analysis...
  34. pmc Alzheimer's disease protein Abeta1-42 does not disrupt isolated synaptic vesicles
    Peter B Allen
    University of Washington, Department of Chemistry, Box 351700, Seattle, WA 98195, USA
    Biochim Biophys Acta 1782:326-34. 2008
    ..From these experiments, we conclude that isolated synaptic vesicles are not disrupted by Abeta1-42...
  35. pmc Sizing subcellular organelles and nanoparticles confined within aqueous droplets
    Jennifer C Gadd
    Department of Chemistry, University of Washington, Box 351700, Seattle, Washington 98195 1700, USA
    Anal Chem 80:3450-7. 2008
    ....
  36. pmc Single-synapse ablation and long-term imaging in live C. elegans
    Peter B Allen
    Department of Chemistry, University of Washington, Box 351700, Seattle, WA 98195 1700, United States
    J Neurosci Methods 173:20-6. 2008
    ..elegans to facilitate long-term fluorescence imaging and observation of neuronal development. With this technique, we directly demonstrated the existence of competition between developing synapses in the HSNL motor neuron...
  37. ncbi request reprint Parametric investigation on the effect of channel topologies on electrophoretic separations
    David S W Lim
    Department of Chemistry, University of Washington, Seattle, WA 98195 1700, USA
    J Chromatogr A 1027:237-44. 2004
    ....

Research Grants26

  1. Probing single proteins in nanofabricated bioenvironment
    Daniel Chiu; Fiscal Year: 2002
    ..Gaining a better understanding of the true in vivo activities of proteins inside cells is critical for developing new drugs that target these proteins for the treatment of devastating diseases. ..
  2. Profiling Biochemical Contents of Single Mitochondria
    Daniel Chiu; Fiscal Year: 2009
    ..While our focus here is on the analysis of individual mitochondria, we believe our method can be applied equally well to the chemical analysis of other subcellular organelles and compartments. ..
  3. Single-Molecule Studies of Synaptic Vesicles
    Daniel Chiu; Fiscal Year: 2009
    ....
  4. High-Resolution Analysis of Synaptic Proteins
    Daniel Chiu; Fiscal Year: 2009
    ..4. To examine the effects of aberrant synaptic activity on vesicular protein composition. ..
  5. Super-Resolution Imaging with Difference Deconvolution Microscopy
    Daniel Chiu; Fiscal Year: 2009
    ..DDM uses comparable experimental parameters (e.g. similar laser powers) as confocal microscopy, and thus is inherently suited to use with fragile samples, such as live mammalian cells. ..
  6. Single-Molecule Studies of Synaptic Vesicles
    Daniel T Chiu; Fiscal Year: 2010
    ..abstract_text> ..
  7. High-Resolution Analysis of Synaptic Proteins
    Daniel T Chiu; Fiscal Year: 2010
    ....
  8. Super-Resolution Imaging with Difference Deconvolution Microscopy
    Daniel T Chiu; Fiscal Year: 2010
    ..DDM uses comparable experimental parameters (e.g. similar laser powers) as confocal microscopy, and thus is inherently suited to use with fragile samples, such as live mammalian cells. ..
  9. High-Resolution Analysis of Synaptic Proteins
    Daniel Chiu; Fiscal Year: 2007
    ..4. To examine the effects of aberrant synaptic activity on vesicular protein composition. ..
  10. Probing single proteins in nanofabricated bioenvironment
    Daniel Chiu; Fiscal Year: 2003
    ..Gaining a better understanding of the true in vivo activities of proteins inside cells is critical for developing new drugs that target these proteins for the treatment of devastating diseases. ..
  11. Profiling individual synaptic vesicles with nanofluidics
    Daniel Chiu; Fiscal Year: 2003
    ....
  12. Probing single proteins in nanofabricated bioenvironment
    Daniel Chiu; Fiscal Year: 2004
    ..Gaining a better understanding of the true in vivo activities of proteins inside cells is critical for developing new drugs that target these proteins for the treatment of devastating diseases. ..
  13. Probing single proteins in nanofabricated bioenvironment
    Daniel Chiu; Fiscal Year: 2003
    ..Gaining a better understanding of the true in vivo activities of proteins inside cells is critical for developing new drugs that target these proteins for the treatment of devastating diseases. ..
  14. Probing single proteins in nanofabricated bioenvironment
    Daniel Chiu; Fiscal Year: 2005
    ..Gaining a better understanding of the true in vivo activities of proteins inside cells is critical for developing new drugs that target these proteins for the treatment of devastating diseases. ..
  15. High-Resolution Analysis of Synaptic Proteins
    Daniel Chiu; Fiscal Year: 2005
    ..4. To examine the effects of aberrant synaptic activity on vesicular protein composition. ..
  16. Probing single proteins in nanofabricated bioenvironment
    Daniel Chiu; Fiscal Year: 2006
    ..Gaining a better understanding of the true in vivo activities of proteins inside cells is critical for developing new drugs that target these proteins for the treatment of devastating diseases. ..
  17. Profiling Biochemical Contents of Single Mitochondria
    Daniel Chiu; Fiscal Year: 2006
    ..While our focus here is on the analysis of individual mitochondria, we believe our method can be applied equally well to the chemical analysis of other subcellular organelles and compartments. ..
  18. High-Resolution Analysis of Synaptic Proteins
    Daniel Chiu; Fiscal Year: 2006
    ..4. To examine the effects of aberrant synaptic activity on vesicular protein composition. ..
  19. Profiling Biochemical Contents of Single Mitochondria
    Daniel Chiu; Fiscal Year: 2007
    ..While our focus here is on the analysis of individual mitochondria, we believe our method can be applied equally well to the chemical analysis of other subcellular organelles and compartments. ..
  20. Spatially and Temporally Resolved Perturbation of Cells
    Daniel Chiu; Fiscal Year: 2007
    ..This method will facilitate the detailed study of signaling pathways that underlie disease processes, such as Alzheimer that is a devastating neurological disease associated with aging. ..
  21. High-Resolution Analysis of Synaptic Proteins
    Daniel T Chiu; Fiscal Year: 2010
    ....