S Chien

Summary

Affiliation: University of California
Country: USA

Publications

  1. ncbi Effects of disturbed flow on endothelial cells
    Shu Chien
    Department of Bioengineering, University of California, San Diego, PFBH room 134, 9500 Gilman Drive, La Jolla, CA 92093 0412, USA
    Ann Biomed Eng 36:554-62. 2008
  2. ncbi Multiple cis-elements mediate shear stress-induced gene expression
    J Y Shyy
    Department of Bioengineering, University of California, La Jolla 92093 0412, USA
    J Biomech 28:1451-7. 1995
  3. ncbi Molecular and mechanical bases of focal lipid accumulation in arterial wall
    Shu Chien
    Department of Bioengineering and Medicine, The Whitaker Institute for Biomedical Engineering, University of California, San Diego, 9500 Gilman Drive, Mail Code 0427, SERF Room 221, La Jolla, CA 92093 0427, USA
    Prog Biophys Mol Biol 83:131-51. 2003
  4. ncbi Effects of mechanical forces on signal transduction and gene expression in endothelial cells
    S Chien
    Department of Bioengineering and Institute for Biomedical Engineering, University of California, San Diego, La Jolla 92093 0412, USA
    Hypertension 31:162-9. 1998
  5. ncbi Integrin-mediated mechanotransduction requires its dynamic interaction with specific extracellular matrix (ECM) ligands
    S Jalali
    Department of Bioengineering and The Whitaker Institute of Biomedical Engineering, University of California, San Diego, La Jolla, CA 92093-0427, USA
    Proc Natl Acad Sci U S A 98:1042-6. 2001
  6. ncbi Effects of morphological patterning on endothelial cell migration
    S Li
    Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA
    Biorheology 38:101-8. 2001
  7. ncbi Effects of flow patterns on endothelial cell migration into a zone of mechanical denudation
    P P Hsu
    Department of Bioengineering, University of California at San Diego, La Jolla, California, 92093-0427, USA
    Biochem Biophys Res Commun 285:751-9. 2001
  8. ncbi LDL-activated p38 in endothelial cells is mediated by Ras
    Y Zhu
    Division of Biomedical Sciences, University of California, Riverside, CA 92521, USA
    Arterioscler Thromb Vasc Biol 21:1159-64. 2001
  9. ncbi DNA microarray analysis of gene expression in endothelial cells in response to 24-h shear stress
    B P Chen
    Department of Bioengineering and The Whitaker Institute of Biomedical Engineering, University of California, San Diego, La Jolla 92093-0427, USA
    Physiol Genomics 7:55-63. 2001
  10. ncbi Shear stress induces a time- and position-dependent increase in endothelial cell membrane fluidity
    P J Butler
    The Whitaker Institute of Biomedical Engineering and Department of Bioengineering, University of California-San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0427, USA
    Am J Physiol Cell Physiol 280:C962-9. 2001

Research Grants

  1. Mechanical and Molecular Bases of Endothelial Remodeling
    Shu Chien; Fiscal Year: 2006

Collaborators

Detail Information

Publications97

  1. ncbi Effects of disturbed flow on endothelial cells
    Shu Chien
    Department of Bioengineering, University of California, San Diego, PFBH room 134, 9500 Gilman Drive, La Jolla, CA 92093 0412, USA
    Ann Biomed Eng 36:554-62. 2008
    ..Understanding of the EC responses to different flow patters helps to elucidate the mechanism of the region-specific localization of atherosclerosis in the arterial system...
  2. ncbi Multiple cis-elements mediate shear stress-induced gene expression
    J Y Shyy
    Department of Bioengineering, University of California, La Jolla 92093 0412, USA
    J Biomech 28:1451-7. 1995
    ..The results of our investigations indicate that the shear stress-induced IE gene expression can be mediated through multiple cis-elements...
  3. ncbi Molecular and mechanical bases of focal lipid accumulation in arterial wall
    Shu Chien
    Department of Bioengineering and Medicine, The Whitaker Institute for Biomedical Engineering, University of California, San Diego, 9500 Gilman Drive, Mail Code 0427, SERF Room 221, La Jolla, CA 92093 0427, USA
    Prog Biophys Mol Biol 83:131-51. 2003
    ....
  4. ncbi Effects of mechanical forces on signal transduction and gene expression in endothelial cells
    S Chien
    Department of Bioengineering and Institute for Biomedical Engineering, University of California, San Diego, La Jolla 92093 0412, USA
    Hypertension 31:162-9. 1998
    ..Studies on the effects of mechanical forces on signal transduction and gene expression provide insights into the molecular mechanisms by which hemodynamic factors regulate vascular physiology, and pathophysiology...
  5. ncbi Integrin-mediated mechanotransduction requires its dynamic interaction with specific extracellular matrix (ECM) ligands
    S Jalali
    Department of Bioengineering and The Whitaker Institute of Biomedical Engineering, University of California, San Diego, La Jolla, CA 92093-0427, USA
    Proc Natl Acad Sci U S A 98:1042-6. 2001
    ..Our results indicate that the dynamic formation of new connections between integrins and their specific ECM ligands is critical in relaying the signals induced by shear stress to intracellular pathways...
  6. ncbi Effects of morphological patterning on endothelial cell migration
    S Li
    Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA
    Biorheology 38:101-8. 2001
    ..The present study provides direct evidence on the role of cell morphology in EC migration, and will help us to understand the mechanisms of EC migration during angiogenesis and wound healing...
  7. ncbi Effects of flow patterns on endothelial cell migration into a zone of mechanical denudation
    P P Hsu
    Department of Bioengineering, University of California at San Diego, La Jolla, California, 92093-0427, USA
    Biochem Biophys Res Commun 285:751-9. 2001
    ..This study has demonstrated that different flow patterns differentially affect EC monolayer migration into the denuded zone involving multiple mechanisms...
  8. ncbi LDL-activated p38 in endothelial cells is mediated by Ras
    Y Zhu
    Division of Biomedical Sciences, University of California, Riverside, CA 92521, USA
    Arterioscler Thromb Vasc Biol 21:1159-64. 2001
    ..Thus, LDL activates both p38 and JNK signaling pathways through Ras activation, and furthermore, these events may play an important role in LDL-induced endothelial activation...
  9. ncbi DNA microarray analysis of gene expression in endothelial cells in response to 24-h shear stress
    B P Chen
    Department of Bioengineering and The Whitaker Institute of Biomedical Engineering, University of California, San Diego, La Jolla 92093-0427, USA
    Physiol Genomics 7:55-63. 2001
    ..These results provide information on the profile of gene expression in shear-adapted ECs, which is the case for the native ECs in the straight part of the aorta in vivo...
  10. ncbi Shear stress induces a time- and position-dependent increase in endothelial cell membrane fluidity
    P J Butler
    The Whitaker Institute of Biomedical Engineering and Department of Bioengineering, University of California-San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0427, USA
    Am J Physiol Cell Physiol 280:C962-9. 2001
    ..These results demonstrate shear-induced changes in membrane fluidity that are time dependent and spatially heterogeneous. These changes in membrane fluidity may have important implications in shear-induced membrane protein modulation...
  11. ncbi Activation of integrins in endothelial cells by fluid shear stress mediates Rho-dependent cytoskeletal alignment
    E Tzima
    Department of Vascular Biology, The Scripps Research Institute, 10550 N.Torrey Pines Road, La Jolla, CA 92037, USA
    EMBO J 20:4639-47. 2001
    ..This transient inhibition is necessary for cytoskeletal alignment in the direction of flow. The results therefore define the role of integrins and Rho in a pathway leading to endothelial cell adaptation to flow...
  12. ncbi Role of Cbl in shear-activation of PI 3-kinase and JNK in endothelial cells
    Hui Miao
    Department of Bioengineering, University of California at San Diego, La Jolla, California 92093 0427, USA
    Biochem Biophys Res Commun 292:892-9. 2002
    ....
  13. ncbi Fluid shear stress activation of focal adhesion kinase. Linking to mitogen-activated protein kinases
    S Li
    Department of Bioengineering and Institute for Biomedical Engineering, University of California, San Diego, La Jolla, California 92093, USA
    J Biol Chem 272:30455-62. 1997
    ..These results demonstrate that FAK signaling is critical in the shear stress-induced dual activation of ERK and JNK...
  14. ncbi Shear stress, SIRT1, and vascular homeostasis
    Zhen Chen
    Division of Biomedical Sciences, Biochemistry and Molecular Biology Graduate Program, University of California, Riverside, CA 92521 0121, USA
    Proc Natl Acad Sci U S A 107:10268-73. 2010
    ..These results suggest that atheroprotective flow, via AMPK and SIRT1, increases NO bioavailability in endothelium...
  15. ncbi Role of integrins in cellular responses to mechanical stress and adhesion
    J Y Shyy
    Department of Bioengineering, University of California, San Diego, La Jolla 92093 0412, USA
    Curr Opin Cell Biol 9:707-13. 1997
    ..Recent findings suggest that integrins may function as mechanotransducers in cells...
  16. ncbi The Dictyostelium LIM domain-containing protein LIM2 is essential for proper chemotaxis and morphogenesis
    S Chien
    Section of Cell and Developmental Biology, Division of Biology, Center for Molecular Genetics, University of California, San Diego, USA
    Mol Biol Cell 11:1275-91. 2000
    ..Our findings may be generally applicable to understanding pathways that control cell movement and morphogenesis in all multicellular organisms. Structure function studies on the LIM domains are presented...
  17. ncbi Leukocyte rolling in rat mesentery venules: distribution of adhesion bonds and the effects of cytoactive agents
    Y Zhao
    The Whitaker Institute of Biomedical Engineering and Department of Bioengineering, University of California, San Diego, La Jolla 92093-0427, USA
    Ann Biomed Eng 29:360-72. 2001
    ..The findings suggest that, in the case of "spontaneous" rolling, there are on average approximately 2-3 clusters of adhesion bonds between a rolling cell and the vessel wall, and approximately five bonds in each cluster...
  18. ncbi Dynamic contact forces on leukocyte microvilli and their penetration of the endothelial glycocalyx
    Y Zhao
    Department of Bioengineering and The Whitaker Institute of Biomedical Engineering, University of California, San Diego, La Jolla 92093, USA
    Biophys J 80:1124-40. 2001
    ..Once tethering is initiated, rolling tends to ensue because F(m)(z) and contact duration will both increase substantially to facilitate glycocalyx penetration by the shorter microvilli...
  19. ncbi Molecular basis of rheological modulation of endothelial functions: importance of stress direction
    Shu Chien
    Department of Bioengineering, Whitaker Institute for Biomedical Engineering, University of California, San Diego, La Jolla, CA 92093 0412, USA
    Biorheology 43:95-116. 2006
    ..Therefore, the direction of stress has important influences on the biorheological effects of flow and deformation on vascular endothelium in health and disease...
  20. ncbi Mechanotransduction and endothelial cell homeostasis: the wisdom of the cell
    Shu Chien
    Dept of Bioengineering, PFBH, Rm 134, Univ of California, San Diego, La Jolla, CA 92093 0412, USA
    Am J Physiol Heart Circ Physiol 292:H1209-24. 2007
    ....
  21. ncbi Molecular basis of the effects of shear stress on vascular endothelial cells
    Yi-Shuan J Li
    Department of Bioengineering, University of California, San Diego, La Jolla, 92093-0412, USA
    J Biomech 38:1949-71. 2005
    ..The knowledge gained on mechano-transduction, with verifications under in vivo conditions, will advance our understanding of the physiological and pathological processes in vascular remodeling and adaptation in health and disease...
  22. ncbi Biochemistry and biomechanics of cell motility
    Song Li
    Department of Bioengineering and Center for Tissue Engineering, University of California, Berkeley, CA 94720, USA
    Annu Rev Biomed Eng 7:105-50. 2005
    ....
  23. ncbi The National Institute of Biomedical Imaging and Bioengineering
    Shu Chien
    Department of Bioengineering, and The Whitaker Institute of Biomedical Engineering, University of California, San Diego, La Jolla, California 92093-0427, USA
    Annu Rev Biomed Eng 6:1-26. 2004
  24. ncbi Molecular basis of mechanical modulation of endothelial cell migration
    Shu Chien
    Department of Bioengineering and Whitaker Institute of Biomedical Engineering, University of California, San Diego, La Jolla, CA, USA
    Front Biosci 10:1985-2000. 2005
    ..Investigations of EC migration under shearing can provide valuable knowledge on vascular remodeling process under physiological and pathological conditions...
  25. ncbi mTOR is the rapamycin-sensitive kinase that confers mechanically-induced phosphorylation of the hydrophobic motif site Thr(389) in p70(S6k)
    Troy Alan Hornberger
    Department of Bioengineering, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0412, USA
    FEBS Lett 581:4562-6. 2007
    ..The results indicate that mTOR is the RS element and reveal that mTOR signaling can be activated through a PI3K-independent mechanism...
  26. ncbi Pulsatile equibiaxial stretch inhibits thrombin-induced RhoA and NF-kappaB activation
    Jason H Haga
    Department of Bioengineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 00435, USA
    Biochem Biophys Res Commun 372:216-20. 2008
    ..These data suggest that stretch, acting through NO and cGMP, can prevent the ability of thrombin to stimulate Rho signaling pathways that contribute to pathophysiological proliferative and inflammatory responses...
  27. ncbi Investigating the role of the extracellular environment in modulating hepatic stellate cell biology with arrayed combinatorial microenvironments
    David A Brafman
    Department of Bioengineering, University of California, San Diego, USA
    Integr Biol (Camb) 1:513-24. 2009
    ..Our results indicate that array-based screens may provide an efficient means for identifying candidate signaling pathways to be targeted for anti-fibrotic therapies...
  28. ncbi Interplay between integrins and FLK-1 in shear stress-induced signaling
    Yingxiao Wang
    Department of Bioengineering and Whitaker Institute of Biomedical Engineering, University of California at San Diego, La Jolla, California 92093, USA
    Am J Physiol Cell Physiol 283:C1540-7. 2002
    ..Integrins are essential for the mechanical activation of Flk-1 by shear stress but not for the chemical activation of Flk-1 by VEGF...
  29. ncbi Rate sensitivity of shear-induced changes in the lateral diffusion of endothelial cell membrane lipids: a role for membrane perturbation in shear-induced MAPK activation
    Peter J Butler
    Department of Bioengineering and The Whitaker Institute of Biomedical Engineering, University of California, San Diego, La Jolla, California 92093, USA
    FASEB J 16:216-8. 2002
    ..These results have implications for understanding the role of t in modulating vascular functions in health and disease...
  30. ncbi Rho mediates the shear-enhancement of endothelial cell migration and traction force generation
    Yan-Ting Shiu
    Department of Bioengineering and The Whitaker Institute of Biomedical Engineering, University of California at San Diego, La Jolla, California 92093-0427, USA
    Biophys J 86:2558-65. 2004
    ..Our results indicate that shear stress enhances the migration speed of endothelial cells by modulating the biophysical force of tractions through the biochemical pathway of Rho-p160ROCK...
  31. ncbi Visualizing the mechanical activation of Src
    Yingxiao Wang
    Department of Bioengineering and The Whitaker Institute of Biomedical Engineering, La Jolla, California 92093, USA
    Nature 434:1040-5. 2005
    ..We find that the transmission of mechanically induced Src activation is a dynamic process that directs signals via the cytoskeleton to spatial destinations...
  32. ncbi The role of the dynamics of focal adhesion kinase in the mechanotaxis of endothelial cells
    Song Li
    Department of Bioengineering and The Whitaker Institute of Biomedical Engineering, University of California at San Diego, La Jolla, CA 92093-0427, USA
    Proc Natl Acad Sci U S A 99:3546-51. 2002
    ..Our results demonstrate the dynamics of FAK at FAs during the directional migration of EC in response to mechanical force, and suggest that mechanotaxis is an important mechanism controlling EC migration...
  33. ncbi Signal transduction in matrix contraction and the migration of vascular smooth muscle cells in three-dimensional matrix
    Song Li
    Department of Bioengineering, University of California, Berkeley, CA 92093, USA
    J Vasc Res 40:378-88. 2003
    ....
  34. ncbi Anisotropic rheology and directional mechanotransduction in vascular endothelial cells
    Juan C Del Alamo
    Department of Mechanical and Aerospace Engineering, University of California at San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 105:15411-6. 2008
    ..The resulting directional anisotropy and spatial inhomogeneity of cytoplasmic rheology may play an important role in mechanotransduction in adherent cells by providing a means to sense the direction of mechanical stimuli...
  35. ncbi Roles of microtubule dynamics and small GTPase Rac in endothelial cell migration and lamellipodium formation under flow
    Ying-Li Hu
    Department of Bioengineering, Whitaker Institute of Biomedical Engineering, University of California-San Diego, La Jolla, CA 92093-0427, USA
    J Vasc Res 39:465-76. 2002
    ..Our findings suggest that MT dynamics and optimum Rac activation are required for the polarized protrusion of lamellipodia that drives the directional EC migration under flow...
  36. ncbi Regulation of endothelial cell cycle by laminar versus oscillatory flow: distinct modes of interactions of AMP-activated protein kinase and Akt pathways
    Deliang Guo
    Division of Biomedical Sciences, University of California, Riverside, CA 92521 0121, USA
    Circ Res 100:564-71. 2007
    ..In contrast, AMPK phosphorylation was higher in the thoracic aorta. These results provide a molecular mechanism by which laminar versus oscillatory flow regulates the endothelial cell cycle...
  37. ncbi Chronotropic response of cultured neonatal rat ventricular myocytes to short-term fluid shear
    Ilka Lorenzen-Schmidt
    Department of Medicine, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0613, USA
    Cell Biochem Biophys 46:113-22. 2006
    ..The results suggest that the beta-adrenergic signaling pathway and integrin activation, which are known to interact, may play an important role in the response mechanism...
  38. ncbi Influence of temperature on rheology of human erythrocytes
    K L Sung
    Institute for Biomedical Engineering, University of California, San Diego, La Jolla 92093 0412
    Chin J Physiol 35:81-94. 1992
    ..These responses to temperature variations provide insights into the viscoelastic properties of the erythrocyte membrane...
  39. ncbi Roles of microfilaments and microtubules in paxillin dynamics
    Ying-Li Hu
    Department of Bioengineering and The Whitaker Institute of Biomedical Engineering, University of California, San Diego, La Jolla, CA 92093, USA
    Biochem Biophys Res Commun 348:1463-71. 2006
    ..Our findings also suggest that tyrosine phosphorylation is an important element in paxillin dynamics at FAs...
  40. ncbi Cooperative effects of Rho and mechanical stretch on stress fiber organization
    Roland Kaunas
    Department of Bioengineering, The Whitaker Institute of Biomedical Engineering, University of California at San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 102:15895-900. 2005
    ..Our results demonstrate that the stretch-induced stress fiber orientation is a function of the interplay between Rho pathway activity and the magnitude of stretching...
  41. ncbi Mechanical stimuli and nutrients regulate rapamycin-sensitive signaling through distinct mechanisms in skeletal muscle
    Troy A Hornberger
    Department of Bioengineering and Whitaker Institute of Biomedical Engineering, University of California, San Diego, 9500 Gilman Dr, La Jolla, California 92093-0412, USA
    J Cell Biochem 97:1207-16. 2006
    ..Taken together, these results indicate that exogenous nutrients are not required for mechanically induced RS signaling and that nutrient and mechanical stimuli activate RS signaling through distinct upstream mechanisms...
  42. ncbi Flow activation of AMP-activated protein kinase in vascular endothelium leads to Krüppel-like factor 2 expression
    Angela Young
    Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093 0412, USA
    Arterioscler Thromb Vasc Biol 29:1902-8. 2009
    ..We investigated whether the PS-induced expression of the transcription factor Krüppel-Like Factor 2 (KLF2) in cultured ECs and its expression in the mouse aorta is regulated by AMP-activated protein kinase (AMPK)...
  43. ncbi Molecular basis of the effects of mechanical stretch on vascular smooth muscle cells
    Jason H Haga
    Department of Bioengineering and Medicine, Whitaker Institute of Biomedical Engineering, University of California, San Diego, La Jolla, CA 92093 0412, USA
    J Biomech 40:947-60. 2007
    ....
  44. ncbi Directional shear flow and Rho activation prevent the endothelial cell apoptosis induced by micropatterned anisotropic geometry
    Chia Ching Wu
    Department of Bioengineering and Whitaker Institute of Biomedical Engineering, University of California at San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 104:1254-9. 2007
    ..These extracellular microenvironment factors affect cell survival through alterations in Rho GTPase activity, stress fiber organization, and FAK phosphorylation...
  45. ncbi Role of shear stress direction in endothelial mechanotransduction
    Shu Chien
    UCSD, La Jolla, CA, USA
    Mol Cell Biomech 5:1-8. 2008
    ..At branch points, the complex flow patterns with little net direction are atherogenic. Thus, the direction of shear stress has important physiological and pathophysiological effects on vascular ECs...
  46. ncbi Rho-ROCK-LIMK-cofilin pathway regulates shear stress activation of sterol regulatory element binding proteins
    Tong Lin
    Division of Biomedical Sciences, University of California, Riverside, Riverside, Calif 92521-0121, USA
    Circ Res 92:1296-304. 2003
    ..By enhancing the SREBP-mediated cholesterol metabolism, this unique mechanism may contribute to endothelial cell functions under flow...
  47. ncbi Effects of cell tension on the small GTPase Rac
    Akira Katsumi
    Division of Vascular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Cell Biol 158:153-64. 2002
    ..We hypothesize that regulation of Rac activity by tension may be important for motility, polarization, and directionality of cell movement...
  48. ncbi Shear stress-induced c-fos activation is mediated by Rho in a calcium-dependent manner
    Yan-Ting Shiu
    Department of Bioengineering and The Whitaker Institute of Biomedical Engineering, University of California at San Diego, La Jolla, CA 92093-0427, USA
    Biochem Biophys Res Commun 303:548-55. 2003
    ..The calcium chelator BAPTA-AM inhibits the shear-induction, as well as the static level, of c-fos activity...
  49. ncbi Activation of Rac1 by shear stress in endothelial cells mediates both cytoskeletal reorganization and effects on gene expression
    Eleni Tzima
    Department of Cell Biology, Division of Vascular Biology, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, CA 92037, USA
    EMBO J 21:6791-800. 2002
    ..These studies provide a unifying model linking three of the main responses to shear stress that mediate both normal adaptation to hemodynamic forces and inflammatory dysfunction of endothelial cells in atherosclerosis...
  50. ncbi Genomic analysis of smooth muscle cells in 3-dimensional collagen matrix
    Song Li
    Department of Bioengineering and The Whitaker Institute of Biomedical Engineering University of California, San Diego, La Jolla, California, USA
    FASEB J 17:97-9. 2003
    ..The information generated in this study helps to elucidate the molecular basis of the modulation of SMC phenotypes by the extracellular matrix...
  51. ncbi Shear stress and VEGF activate IKK via the Flk-1/Cbl/Akt signaling pathway
    Yingxiao Wang
    Department of Bioengineering, The Whitaker Institute of Biomedical Engineering, University of California San Diego, La Jolla 92093 0427, USA
    Am J Physiol Heart Circ Physiol 286:H685-92. 2004
    ..Therefore, our results indicate that the signaling events induced by shear stress and VEGF converge at the membrane receptor Flk-1 and that these stimuli share the Flk-1/Cbl/Akt pathway in activating IKK activation...
  52. ncbi Control of neutrophil pseudopods by fluid shear: role of Rho family GTPases
    Ayako Makino
    Dept. of Bioengineering, The Whitaker Institute of Biomedical Engineering, University of California, San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA
    Am J Physiol Cell Physiol 288:C863-71. 2005
    ..These results suggest that deactivation of Rac activity by fluid shear plays an important role in stable circulation of leukocytes...
  53. ncbi The role of phospholipase D and phosphatidic acid in the mechanical activation of mTOR signaling in skeletal muscle
    T A Hornberger
    Department of Bioengineering and Whitaker Institute of Biomedical Engineering, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0412, USA
    Proc Natl Acad Sci U S A 103:4741-6. 2006
    ..Because rapamycin and PA compete for binding to the FRB domain on mTOR, these results suggest that mechanical stimuli activate mTOR signaling through an enhanced binding of PA to the FRB domain on mTOR...
  54. ncbi Effects of flow patterns on the localization and expression of VE-cadherin at vascular endothelial cell junctions: in vivo and in vitro investigations
    Hui Miao
    Department of Bioengineering, The Whitaker Institute of Biomedical Engineering, University of California, San Diego, La Jolla, CA 92093-0412, USA
    J Vasc Res 42:77-89. 2005
    ....
  55. ncbi In vivo knee moments and shear after total knee arthroplasty
    DARRYL D D'LIMA
    Shiley Center for Orthopaedic Research and Education, Scripps Clinic, 11025 N Torrey Pines Road, Suite 140, La Jolla, CA 92037, USA Department of Bioengineering, University of California, San Diego, CA, USA
    J Biomech 40:S11-7. 2007
    ..These findings indicate that the soft tissues around the knee absorbed most of the external shear forces. Our results highlight the importance of direct measurements of knee forces...
  56. ncbi Dynamic motion of paxillin on actin filaments in living endothelial cells
    Ying Li Hu
    Department of Bioengineering and The Whitaker Institute of Biomedical Engineering, University of California, San Diego, La Jolla, CA 92093, USA
    Biochem Biophys Res Commun 357:871-6. 2007
    ....
  57. ncbi Regulation of mTOR by mechanically induced signaling events in skeletal muscle
    Troy Alan Hornberger
    Department of Bioengineering and Whitaker Institute of Biomedical Engineering, University of California San Diego, La Jolla, California, USA
    Cell Cycle 5:1391-6. 2006
    ....
  58. ncbi Shear stress regulation of Krüppel-like factor 2 expression is flow pattern-specific
    Nanping Wang
    Department of Bioengineering, Whitaker Institute of Biomedical Engineering, University of California San Diego, La Jolla, CA 92093 0412, USA
    Biochem Biophys Res Commun 341:1244-51. 2006
    ..In conclusion, differential regulation of KLF2 may mediate the distinct vascular effects induced by various patterns of shear stress...
  59. ncbi The role of p53 deacetylation in p21Waf1 regulation by laminar flow
    Lingfang Zeng
    Division of Biomedical Sciences, University of California, Riverside 92521, USA
    J Biol Chem 278:24594-9. 2003
    ..The regulation of the p53-p21Waf1 pathway by laminar flow was further supported by observations that flow caused an increase of p21Waf1 level in the wild-type HCT116 (p53+/+) cells but not in the p53-null HCT116 cells...
  60. ncbi Improved significance test for DNA microarray data: temporal effects of shear stress on endothelial genes
    Yihua Zhao
    Department of Bioengineering and The Whitaker Institute of Biomedical Engineering, University of California, San Diego, La Jolla 92093-0427, USA
    Physiol Genomics 12:1-11. 2002
    ..This new significance test may have broad application for the analysis of gene-expression studies and, in fact, to other biological studies in general...
  61. ncbi Role of microRNA-23b in flow-regulation of Rb phosphorylation and endothelial cell growth
    Kuei Chun Wang
    Department of Bioengineering and Institute of Engineering in Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 107:3234-9. 2010
    ..Our findings indicate that PS suppresses EC proliferation through the regulation of miR-23b and provide insights into the role of miRs in mechanotransduction...
  62. ncbi Induction of apoptosis in vascular smooth muscle cells by mechanical stretch
    Mohammad Sotoudeh
    Department of Bioengineering and Whitaker Institute of Biomedical Engineering, Advanced Tissue Sciences, La Jolla, California 92093, USA
    Am J Physiol Heart Circ Physiol 282:H1709-16. 2002
    ..These results support the hypothesis that circumferential overload under hypertensive conditions induces a clustering of death receptors that cause vascular smooth muscle cell apoptosis...
  63. ncbi Stent implantation activates Akt in the vessel wall: role of mechanical stretch in vascular smooth muscle cells
    Rui-Hai Zhou
    Division of Biomedical Sciences, University of California, Riverside, Riverside, Calif 92521-0121, USA
    Arterioscler Thromb Vasc Biol 23:2015-20. 2003
    ..CONCLUSIONS: Mechanical stretch of the vascular wall during stent deployment may contribute to ISR by activating the Akt pathway...
  64. ncbi Shear stress activation of SREBP1 in endothelial cells is mediated by integrins
    Yi Liu
    Department of Bioengineering and Whitaker Institute of Biomedical Engineering, University of California at San Diego, La Jolla, USA
    Arterioscler Thromb Vasc Biol 22:76-81. 2002
    ..These results demonstrate that integrin signaling plays a critical role in the modulation of SREBP in ECs in response to shear stress...
  65. ncbi Combinatorial signaling microenvironments for studying stem cell fate
    Christopher J Flaim
    Department of Bioengineering and Medicine, University of California San Diego, La Jolla, CA 92093 0412, USA
    Stem Cells Dev 17:29-39. 2008
    ..This versatile technique is compatible with virtually any set of insoluble and soluble cues, leverages existing software and hardware, and represents a step toward developing the 'systems biology' of stem cells...
  66. ncbi Differential regulation of Rho GTPases by beta1 and beta3 integrins: the role of an extracellular domain of integrin in intracellular signaling
    Hui Miao
    Department of Bioengineering and The Whitaker Institute of Biomedical Engineering, University of California, San Diego, La Jolla 92093-0427, USA
    J Cell Sci 115:2199-206. 2002
    ....
  67. ncbi microRNA: a master regulator of cellular processes for bioengineering systems
    Wei Sun
    Division of Biomedical Sciences, University of California, Riverside, California 92521, USA
    Annu Rev Biomed Eng 12:1-27. 2010
    ....
  68. ncbi Defining long-term maintenance conditions of human embryonic stem cells with arrayed cellular microenvironment technology
    David A Brafman
    Department of Bioengineering, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0412, USA
    Stem Cells Dev 18:1141-54. 2009
    ..In the future, the novel array platform and analysis procedure presented here will be applied toward the directed differentiation of hESCs and maintenance of other stem and progenitor cell populations...
  69. ncbi Selective adapter recruitment and differential signaling networks by VEGF vs. shear stress
    Yingxiao Wang
    Department of Bioengineering and The Whitaker Institute of Biomedical Engineering, University of California at San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 104:8875-9. 2007
    ..g., ERK and JNK...
  70. ncbi The spatiotemporal pattern of Src activation at lipid rafts revealed by diffusion-corrected FRET imaging
    Shaoying Lu
    Department of Bioengineering, University of Illinois at Urbana Champaign, Urbana, Illinois, USA
    PLoS Comput Biol 4:e1000127. 2008
    ..Our FE-based method also provides an integrated platform of image analysis for studying molecular mobility and reconstructing the spatiotemporal activation maps of signaling molecules in live cells...
  71. ncbi An extracellular matrix microarray for probing cellular differentiation
    Christopher J Flaim
    Departments of Bioengineering and Medicine, University of California San Diego, 9500 Gilman Drive- MC 0412, La Jolla, California 92093-0412, USA
    Nat Methods 2:119-25. 2005
    ..This versatile technique can be easily adapted to other applications, as it is amenable to studying almost any insoluble microenvironmental cue in a combinatorial fashion and is compatible with several cell types...
  72. ncbi Integrins regulate VE-cadherin and catenins: dependence of this regulation on Src, but not on Ras
    Yingxiao Wang
    Department of Bioengineering, The Whitaker Institute of Biomedical Engineering, University of California at San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 103:1774-9. 2006
    ..The results indicate that integrin engagement disrupts VE-cadherin-containing adherens junctions via the activation of Src, but not Ras, possibly as a result of modulation of the actin network...
  73. ncbi Wild type p53 gene causes reorganization of cytoskeleton and, therefore, the impaired deformability and difficult migration of murine erythroleukemia cells
    Weijuan Yao
    School of Basic Medical Sciences, Peking University, Beijing, Peoples Republic of China
    Cell Motil Cytoskeleton 56:1-12. 2003
    ..The present study on the molecular biology with biophysics of MEL cells contributes to our knowledge on the tumor suppressor gene p53...
  74. ncbi Suppression of c-Cbl tyrosine phosphorylation inhibits neointimal formation in balloon-injured rat arteries
    Zhihui Tang
    Institute of Cardiovascular Science, Peking University Health Science Center, Beijing 100083, China
    Circulation 118:764-72. 2008
    ..In the present study, we examined the role of c-Cbl tyrosine phosphorylation in vascular injury...
  75. ncbi Synergism of biochemical and mechanical stimuli in the differentiation of human placenta-derived multipotent cells into endothelial cells
    Chia Ching Wu
    Cardiovascular and Blood Research Center, National Health Research Institutes, Miaoli, Taiwan, Republic of China
    J Biomech 41:813-21. 2008
    ..Our findings suggest that the combined use of endothelial growth factors and high shear stress is synergistic for the endothelial differentiation of PDMCs...
  76. ncbi Localized alpha4 integrin phosphorylation directs shear stress-induced endothelial cell alignment
    Lawrence E Goldfinger
    Sol Sherry Thrombosis Research Center and Department of Anatomy and Cell Biology, Temple University School of Medicine, 3400 N Broad Street, OMS 415, Philadelphia, PA 19140, USA
    Circ Res 103:177-85. 2008
    ..We thereby show that shear-induced PKA-dependent alpha4 integrin phosphorylation at the downstream edge of endothelial cells promotes localized Rac1 activation, which in turn directs cytoskeletal alignment in response to shear stress...
  77. ncbi Integrin-mediated expression of bone formation-related genes in osteoblast-like cells in response to fluid shear stress: roles of extracellular matrix, Shc, and mitogen-activated protein kinase
    Ding Yu Lee
    Institute of Life Sciences, National Defense Medical Center, Division of Medical Engineering Research, National Health Research Institutes, Taiwan, China
    J Bone Miner Res 23:1140-9. 2008
    ..The beta(1)/Shc association leads to the activation of ERK, which is critical for shear induction of bone formation-related genes in osteoblast-like cells...
  78. ncbi Role of integrins in endothelial mechanosensing of shear stress
    John Y J Shyy
    Division of Biomedical Sciences, University of California Riverside, Riverside, Calif 92521 0121, USA
    Circ Res 91:769-75. 2002
    ..These studies show that integrins play an important role in mechanosensing in addition to their involvement in cell attachment and migration...
  79. ncbi Tumor cell cycle arrest induced by shear stress: Roles of integrins and Smad
    Shun Fu Chang
    Department of Biological Science and Technology, National Chiao Tung University, Hsinchu 300, Taiwan
    Proc Natl Acad Sci U S A 105:3927-32. 2008
    ....
  80. ncbi TRAIL gene reorganizes the cytoskeleton and decreases the motility of human leukemic Jurkat cells
    Yuhui Jiang
    Department of Biophysics, Health Science Center, Peking University, Beijing, People's Republic of China
    Cell Motil Cytoskeleton 63:471-82. 2006
    ..These findings contribute to the knowledge on the role of TRAIL in tumor metastasis and provide mechanistic basis for the clinical application of TRAIL and tumor therapy...
  81. ncbi Laminar flow activates peroxisome proliferator-activated receptor-gamma in vascular endothelial cells
    Yi Liu
    Division of Biomedical Sciences, University of California at Riverside, Riverside, CA 92521-0121, USA
    Circulation 110:1128-33. 2004
    ..The flow production of PPARgamma ligands is through the PLA2-CYP450 pathway, and the induced PPARgamma ligands exert antiinflammatory effects in several types of cells...
  82. ncbi Shear stress increases ICAM-1 and decreases VCAM-1 and E-selectin expressions induced by tumor necrosis factor-[alpha] in endothelial cells
    Jeng Jiann Chiu
    Division of Medical Engineering Research, National Health Research Institutes, Taipei 114, Taiwan, ROC
    Arterioscler Thromb Vasc Biol 24:73-9. 2004
    ..Vascular endothelial cells (ECs) are subjected to shear stress and cytokine stimulation. We studied the interplay between shear stress and cytokine in modulating the expression of adhesion molecule genes in ECs...
  83. ncbi Shear stress regulates gene expression in vascular endothelial cells in response to tumor necrosis factor-alpha: a study of the transcription profile with complementary DNA microarray
    Jeng Jiann Chiu
    Division of Medical Engineering Research, National Health Research Institutes, Miaoli, 350, Taiwan, ROC
    J Biomed Sci 12:481-502. 2005
    ..Our findings suggest that laminar shear stress serves protective functions against atherogenesis...
  84. ncbi Analysis of the effect of disturbed flow on monocytic adhesion to endothelial cells
    Jeng Jiann Chiu
    Division of Medical Engineering Research, National Health Research Institutes, Taipei 114, Taiwan, ROC
    J Biomech 36:1883-95. 2003
    ..Our findings demonstrate the importance of complex flow environment in modulating the adhesive properties of vascular endothelium and consequently monocyte adhesion in regions of prevalence of atherosclerotic lesions...
  85. ncbi TFAR19 gene changes the biophysical properties of murine erythroleukemia cells
    Li Gu
    Department of Biophysics, Health Science Center, Peking University, Beijing 100083, P. R. China
    Cell Biochem Biophys 43:355-63. 2005
    ..These findings suggest the potential of TFAR19 for antitumor cell migration, and thus for antitumor gene therapy...
  86. ncbi Phosphatidylinositol 3-kinase/Akt pathway is involved in transforming growth factor-beta1-induced phenotypic modulation of 10T1/2 cells to smooth muscle cells
    Sheng Chieh Lien
    Division of Medical Engineering Research, National Health Research Institutes, Miaoli 350, Taiwan, ROC
    Cell Signal 18:1270-8. 2006
    ..These results indicate that TGF-beta1 is capable of inducing the SMC phenotype of 10T1/2 cells and that this induction is mediated through the PI3K/Akt signaling pathway...
  87. ncbi Roles of MAP kinases in the regulation of bone matrix gene expressions in human osteoblasts by oscillatory fluid flow
    Chia-Ching Wu
    Institute of Biomedical Engineering, National Cheng Kung University, Tainan, Taiwan
    J Cell Biochem 98:632-41. 2006
    ..These results suggest that the flow-induction of OPN was mediated through the ERK-Elk1-OPN pathway, and that COL1 was regulated by both the ERK-Elk1-COL1 and JNK-c-JUN-COL1 pathway...
  88. ncbi Synergistic roles of platelet-derived growth factor-BB and interleukin-1beta in phenotypic modulation of human aortic smooth muscle cells
    Cheng Nan Chen
    Division of Medical Engineering Research, National Health Research Institutes Zhunan Campus, Miaoli 350, Taiwan, Republic of China
    Proc Natl Acad Sci U S A 103:2665-70. 2006
    ..Our findings provide insights into the mechanism of SMC phenotypic modulation from contractile to synthetic, e.g., in atherosclerosis...
  89. ncbi Mechanical and chemical regulation of endothelial cell polarity
    Shu Chien
    Circ Res 98:863-5. 2006
  90. ncbi Tumor-derived factors impaired motility and immune functions of dendritic cells through derangement of biophysical characteristics and reorganization of cytoskeleton
    Zhu Zeng
    Department of Biophysics, Health Science Center, Peking University, Beijing, People s Republic of China
    Cell Motil Cytoskeleton 64:186-98. 2007
    ..These results provide insights into the importance of the reconstruction of tumor microenvironment for immunotherapy based on the anti-cancer activities of DCs...
  91. ncbi Shear stress inhibits adhesion molecule expression in vascular endothelial cells induced by coculture with smooth muscle cells
    Jeng Jiann Chiu
    Division of Medical Engineering Research, National Health Research Institutes, Taipei, Taiwan, Republic of China
    Blood 101:2667-74. 2003
    ..These coculture effects are inhibited by shear stress and require specific interaction between ECs and SMCs in close contact...
  92. ncbi Interactions of mechanotransduction pathways
    Vénus Labrador
    Mechanobiology of Cells and Tissues, UMR CNRS 7563 Medicine Faculty, Vandoeuvre, France
    Biorheology 40:47-52. 2003
    ..Second, shear-induced JNK activation is regulated by multiple mechano-sensing mechanisms such as integrin, G-protein and calcium concentration...
  93. ncbi Laminar shear stress up-regulates the expression of stearoyl-CoA desaturase-1 in vascular endothelial cells
    Xiaomei Qin
    Institute of Cardiovascular Science and Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Peking University, Beijing, 100083, PR China
    Cardiovasc Res 74:506-14. 2007
    ..Stearoyl-CoA desaturase-1 (SCD1), which catalyzes the biosynthesis of monounsaturated fatty acids, affects the lipid composition and fluidity of the cell membrane. Thus, we examined the effect of laminar flow on SCD1 expression in ECs...
  94. ncbi Mechanisms of induction of endothelial cell E-selectin expression by smooth muscle cells and its inhibition by shear stress
    Jeng Jiann Chiu
    Division of Medical Engineering Research, National Health Research Institutes, Miaoli, Taiwan, Republic of China
    Blood 110:519-28. 2007
    ..Our findings have elucidated the molecular mechanisms underlying the SMC induction of EC E-selectin expression and the shear stress protection against this SMC induction...
  95. ncbi Regulation of stretch-induced JNK activation by stress fiber orientation
    Roland Kaunas
    Whitaker Institute of Biomedical Engineering, and Department of Bioengineering, Texas A and M University, College Station, TX 77843, USA
    Cell Signal 18:1924-31. 2006
    ..These results suggest that stress fiber orientation perpendicular to the direction of stretch provides a mechanism for both structural and biochemical adaptation to cyclic mechanical stretch...
  96. ncbi The National Institute of Biomedical Imaging and Bioengineering: history, status, and potential impact
    William R Hendee
    Medical College of Wisconsin, Milwaukee 53226, USA
    Ann Biomed Eng 30:2-10. 2002
    ....
  97. ncbi Shear stress inhibits smooth muscle cell-induced inflammatory gene expression in endothelial cells: role of NF-kappaB
    Jeng Jiann Chiu
    Division of Medical Engineering Research, National Health Research Institutes, Miaoli, Taiwan, Republic of China
    Arterioscler Thromb Vasc Biol 25:963-9. 2005
    ..We investigated the inflammation-relevant gene expression in EC/SMC cocultures under static condition and in response to shear stress...

Research Grants1

  1. Mechanical and Molecular Bases of Endothelial Remodeling
    Shu Chien; Fiscal Year: 2006
    ....