Alberto Chiarugi

Summary

Affiliation: University of Massachusetts Medical School
Country: USA

Publications

  1. ncbi request reprint Cell biology. PARP-1--a perpetrator of apoptotic cell death?
    Alberto Chiarugi
    Department of Preclinical and Clinical Pharmacology, University of Florence, 50139 Florence, Italy
    Science 297:200-1. 2002
  2. pmc Inhibitors of poly(ADP-ribose) polymerase-1 suppress transcriptional activation in lymphocytes and ameliorate autoimmune encephalomyelitis in rats
    Alberto Chiarugi
    Department of Preclinical and Clinical Pharmacology, University of Florence, Florence, Italy
    Br J Pharmacol 137:761-70. 2002
  3. pmc Glucose deprivation converts poly(ADP-ribose) polymerase-1 hyperactivation into a transient energy-producing process
    Daniela Buonvicino
    From the Department of Health Sciences, Section of Clinical Pharmacology and Oncology, University of Florence, 50139 Florence, Italy and
    J Biol Chem 288:36530-7. 2013
  4. ncbi request reprint Histone deacetylase (HDAC) inhibitors reduce the glial inflammatory response in vitro and in vivo
    Giuseppe Faraco
    Department of Preclinical and Clinical Pharmacology, University of Florence, Florence, Italy
    Neurobiol Dis 36:269-79. 2009
  5. pmc Inhibition of nicotinamide phosphoribosyltransferase: cellular bioenergetics reveals a mitochondrial insensitive NAD pool
    Maria Pittelli
    Department of Preclinical and Clinical Pharmacology, University of Florence, 50139 Florence, Italy
    J Biol Chem 285:34106-14. 2010
  6. doi request reprint Pharmacological effects of exogenous NAD on mitochondrial bioenergetics, DNA repair, and apoptosis
    Maria Pittelli
    Department of Preclinical and Clinical Pharmacology, University of Florence, Florence, Italy
    Mol Pharmacol 80:1136-46. 2011
  7. ncbi request reprint Poly(ADP-ribose) accumulation and enhancement of postischemic brain damage in 110-kDa poly(ADP-ribose) glycohydrolase null mice
    Andrea Cozzi
    Department of Pharmacology, University of Florence, Firenze, Italy
    J Cereb Blood Flow Metab 26:684-95. 2006
  8. pmc Poly(ADP-ribose) catabolism triggers AMP-dependent mitochondrial energy failure
    Laura Formentini
    Department of Preclinical and Clinical Pharmacology, University of Florence, 50139 Firenze, Italy
    J Biol Chem 284:17668-76. 2009
  9. doi request reprint PARP-1 inhibition prevents CNS migration of dendritic cells during EAE, suppressing the encephalitogenic response and relapse severity
    Leonardo Cavone
    Department of Pharmacology, University of Florence, Florence, Italy
    Mult Scler 17:794-807. 2011
  10. ncbi request reprint Neither energy collapse nor transcription underlie in vitro neurotoxicity of poly(ADP-ribose) polymerase hyper-activation
    Silvia Fossati
    Department of Pharmacology, University of Florence, Florence, Viale Pieraccini 6, 50139 Firenze, Italy
    Neurochem Int 50:203-10. 2007

Collaborators

Detail Information

Publications44

  1. ncbi request reprint Cell biology. PARP-1--a perpetrator of apoptotic cell death?
    Alberto Chiarugi
    Department of Preclinical and Clinical Pharmacology, University of Florence, 50139 Florence, Italy
    Science 297:200-1. 2002
  2. pmc Inhibitors of poly(ADP-ribose) polymerase-1 suppress transcriptional activation in lymphocytes and ameliorate autoimmune encephalomyelitis in rats
    Alberto Chiarugi
    Department of Preclinical and Clinical Pharmacology, University of Florence, Florence, Italy
    Br J Pharmacol 137:761-70. 2002
    ..5. These findings indicate that PARP-1 promotes transcriptional activation in lymphocytes and inhibitors of its enzymatic activity are useful for the treatment of autoimmune disorders of the central nervous system...
  3. pmc Glucose deprivation converts poly(ADP-ribose) polymerase-1 hyperactivation into a transient energy-producing process
    Daniela Buonvicino
    From the Department of Health Sciences, Section of Clinical Pharmacology and Oncology, University of Florence, 50139 Florence, Italy and
    J Biol Chem 288:36530-7. 2013
    ..Overall, the present study identifies basal ADP content and adenylate kinase as key determinants of bioenergetics during PARP-1 hyperactivation and unequivocally demonstrates that ATP loss is not metabolically related to NAD depletion. ..
  4. ncbi request reprint Histone deacetylase (HDAC) inhibitors reduce the glial inflammatory response in vitro and in vivo
    Giuseppe Faraco
    Department of Preclinical and Clinical Pharmacology, University of Florence, Florence, Italy
    Neurobiol Dis 36:269-79. 2009
    ..Data demonstrate that mouse glial cells have ongoing HDAC activity, and its inhibition suppresses the neuroinflammatory response because of a direct impairment of the transcriptional machinery...
  5. pmc Inhibition of nicotinamide phosphoribosyltransferase: cellular bioenergetics reveals a mitochondrial insensitive NAD pool
    Maria Pittelli
    Department of Preclinical and Clinical Pharmacology, University of Florence, 50139 Florence, Italy
    J Biol Chem 285:34106-14. 2010
    ..For the first time, our data indicate that mitochondria lack the canonical NAD rescue pathway, broadening current understanding of cellular bioenergetics...
  6. doi request reprint Pharmacological effects of exogenous NAD on mitochondrial bioenergetics, DNA repair, and apoptosis
    Maria Pittelli
    Department of Preclinical and Clinical Pharmacology, University of Florence, Florence, Italy
    Mol Pharmacol 80:1136-46. 2011
    ....
  7. ncbi request reprint Poly(ADP-ribose) accumulation and enhancement of postischemic brain damage in 110-kDa poly(ADP-ribose) glycohydrolase null mice
    Andrea Cozzi
    Department of Pharmacology, University of Florence, Firenze, Italy
    J Cereb Blood Flow Metab 26:684-95. 2006
    ..Our findings show that PAR accumulation worsens ischemic brain injury, and highlight the therapeutic potential of strategies capable of maintaining PAR homeostasis...
  8. pmc Poly(ADP-ribose) catabolism triggers AMP-dependent mitochondrial energy failure
    Laura Formentini
    Department of Preclinical and Clinical Pharmacology, University of Florence, 50139 Firenze, Italy
    J Biol Chem 284:17668-76. 2009
    ..Also, these data disclose unanticipated AMP-dependent impairment of mitochondrial exchange of adenine nucleotides, which can be of relevance to organelle functioning and disease pathogenesis...
  9. doi request reprint PARP-1 inhibition prevents CNS migration of dendritic cells during EAE, suppressing the encephalitogenic response and relapse severity
    Leonardo Cavone
    Department of Pharmacology, University of Florence, Florence, Italy
    Mult Scler 17:794-807. 2011
    ....
  10. ncbi request reprint Neither energy collapse nor transcription underlie in vitro neurotoxicity of poly(ADP-ribose) polymerase hyper-activation
    Silvia Fossati
    Department of Pharmacology, University of Florence, Florence, Viale Pieraccini 6, 50139 Firenze, Italy
    Neurochem Int 50:203-10. 2007
    ....
  11. doi request reprint Mild activation of poly(ADP-ribose) polymerase (PARP) is neuroprotective in rat hippocampal slice models of ischemic tolerance
    Elisabetta Gerace
    Department of Preclinical and Clinical Pharmacology, University of Florence, Viale G Pieraccini 6, 50139 Florence, Italy
    Eur J Neurosci 36:1993-2005. 2012
    ..Our results suggest that preconditioning with appropriate pharmacological stimuli may promote neuroprotective mechanisms triggered by the sublethal activation of two otherwise deleterious executioners such as PARP and caspase-3/7...
  12. doi request reprint Brain ischemic preconditioning does not require PARP-1
    Giuseppe Faraco
    Department of Pharmacology, University of Florence, Italy
    Stroke 41:181-3. 2010
    ..PARP-1 KO mice were similarly protected by the 5-minute BCCAO...
  13. pmc Neurological basis of AMP-dependent thermoregulation and its relevance to central and peripheral hyperthermia
    Mirko Muzzi
    Department of Preclinical and Clinical Pharmacology, University of Florence, Florence, Italy
    J Cereb Blood Flow Metab 33:183-90. 2013
    ..Together, data disclose the role of AMP, 5'-nucleotidase, and A1R in hypothalamic thermoregulation, as well and their therapeutic relevance to treatment of febrile illness...
  14. doi request reprint 18β-glycyrrhetic acid inhibits immune activation triggered by HMGB1, a pro-inflammatory protein found in the tear fluid during conjunctivitis and blepharitis
    Leonardo Cavone
    Department of Preclinical and Clinical Pharmacology, University of Florence, Florence, Italy
    Ocul Immunol Inflamm 19:180-5. 2011
    ..Evidence indicates that extracellularly released high-mobility group box 1 protein (HMGB1) behaves as a cytokine, promoting inflammation and disease pathogenesis. HMGB1 release occurs during endophtalmitis or uveoretinitis...
  15. doi request reprint Detection and pharmacological modulation of nicotinamide mononucleotide (NMN) in vitro and in vivo
    Laura Formentini
    Department of Preclinical and Clinical Pharmacology, University of Florence, Italy
    Biochem Pharmacol 77:1612-20. 2009
    ..Data provide new information on the biochemistry and pharmacology of NAD biosynthesis, allowing a better understanding of pyridine nucleotide metabolism...
  16. ncbi request reprint Nuclear poly(ADP-ribose) polymerase-1 rapidly triggers mitochondrial dysfunction
    Giulia Cipriani
    Department of Pharmacology, University of Florence, 50139 Florence, Italy
    J Biol Chem 280:17227-34. 2005
    ..Together, our data provide evidence for rapid nucleus-mitochondria cross-talk during hyper-poly(ADP-ribosyl)ation-dependent cell death...
  17. ncbi request reprint Pharmacological inhibition of histone deacetylases by suberoylanilide hydroxamic acid specifically alters gene expression and reduces ischemic injury in the mouse brain
    Giuseppe Faraco
    Department of Preclinical and Pharmacology, University of Florence, Viale Pieraccini 6, 50139 Firenze, Italy
    Mol Pharmacol 70:1876-84. 2006
    ..Data demonstrate that pharmacological inhibition of HDACs promotes expression of neuroprotective proteins within the ischemic brain and underscores the therapeutic potential of molecules inhibiting HDACs for stroke therapy...
  18. pmc Ischemic neuroprotection by TRPV1 receptor-induced hypothermia
    Mirko Muzzi
    Department of Pharmacology, University of Florence, Viale Pieraccini 6, Florence, Italy
    J Cereb Blood Flow Metab 32:978-82. 2012
    ..Data indicate that TRPV1 receptor agonists are promising candidates for hypothermic treatment of stroke...
  19. ncbi request reprint Carboxymethyl beta-glucan binds to corneal epithelial cells and increases cell adhesion to laminin and resistance to oxidative stress
    Marco Porcu
    Department of Pharmacology, University of Florence, Florence, Italy
    Cornea 26:73-9. 2007
    ..In this study, we investigated the effects of carboxymethyl beta-glucan (CMG), a water-soluble derivative of yeast beta-glucan, on cultured rabbit corneal epithelial cells...
  20. doi request reprint Post-ischemic brain damage: targeting PARP-1 within the ischemic neurovascular units as a realistic avenue to stroke treatment
    Flavio Moroni
    Department of Preclinical and Clinical Pharmacology, University of Florence, Italy
    FEBS J 276:36-45. 2009
    ....
  21. pmc Insight into molecular and functional properties of NMNAT3 reveals new hints of NAD homeostasis within human mitochondria
    Roberta Felici
    Department of Health Sciences, University of Florence, Florence, Italy
    PLoS ONE 8:e76938. 2013
    ..Data of the present study modify the scenario of the origin of mitochondrial NAD by showing that, in human cells, NMNAT3 is absent in mitochondria, and, akin to plants and yeast, cytosolic NAD maintains the mitochondrial NAD pool. ..
  22. pmc AMP-dependent hypothermia affords protection from ischemic brain injury
    Mirko Muzzi
    Department of Preclinical and Clinical Pharmacology University of Florence, Florence, Italy
    J Cereb Blood Flow Metab 33:171-4. 2013
    ..Data disclose that AMP-dependent hypothermia is of therapeutic relevance to treatment of brain ischemia...
  23. doi request reprint Nicotinamide phosphoribosyltransferase (NAMPT) activity is essential for survival of resting lymphocytes
    Maria Pittelli
    Section of Clinical Pharmacology and Oncology, Department of Health Sciences, University of Florence, Florence, Italy
    Immunol Cell Biol 92:191-9. 2014
    ..Data demonstrate a unique sensitivity of resting lymphocytes to NAD-depleting agents, providing new hints of relevance to lymphocyte biology and therapeutic interventions with NAMPT inhibitors. ..
  24. doi request reprint Poly(ADP-ribose) polymerase-1 is a nuclear epigenetic regulator of mitochondrial DNA repair and transcription
    Andrea Lapucci
    Department of Preclinical and Clinical Pharmacology, University of Florence, Florence, Italy
    Mol Pharmacol 79:932-40. 2011
    ..These data might have important implications in pharmacology of PARP-1 inhibitors as well as clinical oncology and aging...
  25. ncbi request reprint Poly(ADP-ribosyl)ation and stroke
    Alberto Chiarugi
    Department of Pharmacology, University of Florence, Viale Pieraccini 6, 50139 Firenze, Italy
    Pharmacol Res 52:15-24. 2005
    ....
  26. ncbi request reprint Intrinsic mechanisms of poly(ADP-ribose) neurotoxicity: three hypotheses
    Alberto Chiarugi
    Department of Pharmacology, University of Florence, Viale Pieraccini 6, 50139 Firenze, Italy
    Neurotoxicology 26:847-55. 2005
    ..This review provides an appraisal of the possible molecular mechanisms underlying PAR neurotoxicity, highlighting the pleiotypic effects of the polymer on neural cells exposed to different stressful conditions...
  27. ncbi request reprint A key role for poly(ADP-ribose) polymerase-1 activity during human dendritic cell maturation
    Alessandra Aldinucci
    Department of Pharmacology, University of Florence, Viale Pieraccini 6, 50139 Florence, Italy
    J Immunol 179:305-12. 2007
    ..Together, data provide evidence for a key role of PARP-1 and poly ADP-ribosylation in DC immunocompetence and underscore the relevance of PARP-1 inhibitors to treatment of immune disorders...
  28. ncbi request reprint "Simple but not simpler": toward a unified picture of energy requirements in cell death
    Alberto Chiarugi
    Department of Pharmacology, University of Florence, Florence, Italy
    FASEB J 19:1783-8. 2005
    ....
  29. ncbi request reprint Relevance of high-mobility group protein box 1 to neurodegeneration
    Silvia Fossati
    Department of Preclinical and Clinical Pharmacology, University of Florence, Florence, Italy
    Int Rev Neurobiol 82:137-48. 2007
    ....
  30. ncbi request reprint Inhibition of poly(ADP-ribose) glycohydrolase by gallotannin selectively up-regulates expression of proinflammatory genes
    Elena Rapizzi
    Department of Biological Chemistry, University of Florence, Italy
    Mol Pharmacol 66:890-8. 2004
    ..Overall, our data provide the first evidence that pharmacological inhibition of PARG leads to PAR-dependent alteration of gene expression profiles in macrophages...
  31. ncbi request reprint Induction of CD83+CD14+ nondendritic antigen-presenting cells by exposure of monocytes to IFN-alpha
    Gianni Gerlini
    Plastic Surgery Unit, Regional Melanoma Referral Center, Tuscan Tumor Institute ITT, Santa Maria Annunziata Hospital, Florence, Italy
    J Immunol 181:2999-3008. 2008
    ..The present findings suggest that IFN-alpha alone promptly generates nondendritic APCs able to stimulate memory immune responses. This may represent an additional mode of action of IFN-alpha in vivo...
  32. doi request reprint The NAD metabolome--a key determinant of cancer cell biology
    Alberto Chiarugi
    Department of Preclinical and Clinical Pharmacology, University of Firenze, 50139, Italy
    Nat Rev Cancer 12:741-52. 2012
    ..This necessity supports the targeting of NAD metabolism as a new therapeutic concept for cancer treatment...
  33. doi request reprint Targeting poly(ADP-ribose) polymerase-1 as a promising approach for immunomodulation in multiple sclerosis?
    Leonardo Cavone
    Department of Preclinical and Clinical Pharmacology, Viale Pieraccini 6, University of Florence, 50139 Florence, Italy
    Trends Mol Med 18:92-100. 2012
    ..PARP-1 inhibitors have already reached the clinical arena as cancer treatments, and observations made in treating these patients could help advance treatments for MS...
  34. ncbi request reprint Tryptophan availability selectively limits NO-synthase induction in macrophages
    Alberto Chiarugi
    Department of Preclinical and Clinical Pharmacology, University of Florence, Viale Pieraccini 6, 50139 Florence, Italy
    J Leukoc Biol 73:172-7. 2003
    ..Our results suggest that a reduced local TRP availability may modify macrophage function and possibly the outcome of immune responses...
  35. ncbi request reprint Succinate dehydrogenase subunit B mutations modify human neuroblastoma cell metabolism and proliferation
    Elena Rapizzi
    Endocrinology Unit, Department of Experimental and Clinical Biomedical Sciences, University of Florence, Viale Pieraccini 6, 50139, Florence, Italy
    Horm Cancer 5:174-84. 2014
    ....
  36. pmc Antidromic vasodilatation and the migraine mechanism
    Pierangelo Geppetti
    Headache Centre, Careggi University Hospital, Department of Preclinical and Clinical Pharmacology, University of Florence, Florence, Italy
    J Headache Pain 13:103-11. 2012
    ....
  37. ncbi request reprint The emerging therapeutic potential of sirtuin-interacting drugs: from cell death to lifespan extension
    Marco Porcu
    Department of Preclinical and Clinical Pharmacology, University of Florence, 50139 Florence, Italy
    Trends Pharmacol Sci 26:94-103. 2005
    ..In this article, we focus on sirtuins and provide an appraisal of current compounds that either activate or inhibit sirtuin activity, highlighting their therapeutic potential for the treatment of human diseases...
  38. ncbi request reprint Poly(ADP-ribose) polymerase as a key player in excitotoxicity and post-ischemic brain damage
    Elena Meli
    Department of Preclinical and Clinical Pharmacology, University of Florence, Viale Pieraccini 6, 50135 Florence, Italy
    Toxicol Lett 139:153-62. 2003
    ..Hence, it appears reasonable to propose PARP inhibitors as useful therapeutic agents in pathological brain conditions were necrosis predominates...
  39. ncbi request reprint Characterization of the molecular events following impairment of NF-kappaB-driven transcription in neurons
    Alberto Chiarugi
    Department of Cellular and Molecular Physiology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Brain Res Mol Brain Res 109:179-88. 2002
    ....
  40. ncbi request reprint Poly(ADP-ribose) polymerase-1 activity promotes NF-kappaB-driven transcription and microglial activation: implication for neurodegenerative disorders
    Alberto Chiarugi
    Department of Neuroscience, Stroke and Neurovascular Regulation Laboratory, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachussets, USA
    J Neurochem 85:306-17. 2003
    ....
  41. ncbi request reprint Novel isoquinolinone-derived inhibitors of poly(ADP-ribose) polymerase-1: pharmacological characterization and neuroprotective effects in an in vitro model of cerebral ischemia
    Alberto Chiarugi
    Dipartimento di Farmacologia Preclinica e Clinica, Universita di Firenze, Viale Pieraccini, 6, 50139 Firenze, Italy
    J Pharmacol Exp Ther 305:943-9. 2003
    ..93, P < 0.01) was observed. Our results suggest that TIQ-A and its derivatives are a new class of neuroprotectants that may be helpful in studies aimed at understanding the involvement of PARP-1 in physiology and pathology...
  42. ncbi request reprint STAT1 is activated in neurons after ischemia and contributes to ischemic brain injury
    Yasushi Takagi
    Stroke and Neurovascular Regulation, Neuroscience Center, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Boston 02129, USA
    J Cereb Blood Flow Metab 22:1311-8. 2002
    ..These results indicate that STAT1 is activated and translocated within ischemic neurons and may contribute to brain injury by regulating transcription and phosphorylation of proteins related to apoptosis and cell death...
  43. ncbi request reprint Poly(ADP-ribosyl)ation regulates heat shock factor-1 activity and the heat shock response in murine fibroblasts
    Silvia Fossati
    International Agency for Research on Cancer, Lyon, France
    Biochem Cell Biol 84:703-12. 2006
    ..The data indicate that poly(ADP-ribosyl)ation finely regulates HSF-1 activity, and emphasize the complex role of PARP-1 in the heat-shock response of mammalian cells...
  44. ncbi request reprint Neuroprotective effects of propofol in models of cerebral ischemia: inhibition of mitochondrial swelling as a possible mechanism
    Chiara Adembri
    Dipartimento di Area Critica Medico Chirurgica, Sezione di Anestesiologia e Terapia Intensiva, Universita di Firenze, Italy
    Anesthesiology 104:80-9. 2006
    ..Moreover, the mechanisms involved in its neuroprotective effects are yet unclear...