Zhe Chen

Summary

Affiliation: University of Texas Southwestern Medical Center
Country: USA

Publications

  1. ncbi Activation of p115-RhoGEF requires direct association of Gα13 and the Dbl homology domain
    Zhe Chen
    Department of Biochemistry, The University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390, USA
    J Biol Chem 287:25490-500. 2012
  2. ncbi Activated RhoA binds to the pleckstrin homology (PH) domain of PDZ-RhoGEF, a potential site for autoregulation
    Zhe Chen
    Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    J Biol Chem 285:21070-81. 2010
  3. ncbi Modulation of a GEF switch: autoinhibition of the intrinsic guanine nucleotide exchange activity of p115-RhoGEF
    Zhe Chen
    Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Protein Sci 20:107-17. 2011
  4. ncbi Recognition of the activated states of Galpha13 by the rgRGS domain of PDZRhoGEF
    Zhe Chen
    Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Structure 16:1532-43. 2008
  5. ncbi Structure of the p115RhoGEF rgRGS domain-Galpha13/i1 chimera complex suggests convergent evolution of a GTPase activator
    Zhe Chen
    Department of Biochemistry, The University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390, USA
    Nat Struct Mol Biol 12:191-7. 2005
  6. ncbi Mapping the Galpha13 binding interface of the rgRGS domain of p115RhoGEF
    Zhe Chen
    Department of Biochemistry, The University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    J Biol Chem 278:9912-9. 2003
  7. ncbi Regulation of Rho guanine nucleotide exchange factors by G proteins
    Paul C Sternweis
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Adv Protein Chem 74:189-228. 2007
  8. ncbi Structural basis of effector regulation and signal termination in heterotrimeric Galpha proteins
    Stephen R Sprang
    Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Adv Protein Chem 74:1-65. 2007

Collaborators

  • S R Sprang
  • Xinlin Du
  • Paul C Sternweis
  • Angela M Carter
  • Ying-Fan Hsiung
  • Ying Fan Hsiung
  • William D Singer
  • Shahab M Danesh

Detail Information

Publications8

  1. ncbi Activation of p115-RhoGEF requires direct association of Gα13 and the Dbl homology domain
    Zhe Chen
    Department of Biochemistry, The University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390, USA
    J Biol Chem 287:25490-500. 2012
    ..This study provides new insight into the mechanism of regulation of the RGS-RhoGEF and broadens our understanding of G protein signaling...
  2. ncbi Activated RhoA binds to the pleckstrin homology (PH) domain of PDZ-RhoGEF, a potential site for autoregulation
    Zhe Chen
    Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    J Biol Chem 285:21070-81. 2010
    ..PH bound to both forms of RhoA can be isolated by size-exclusion chromatography. This novel interaction between activated RhoA and PH could play a key role in regulation of RhoGEF activity in vivo...
  3. ncbi Modulation of a GEF switch: autoinhibition of the intrinsic guanine nucleotide exchange activity of p115-RhoGEF
    Zhe Chen
    Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Protein Sci 20:107-17. 2011
    ..It is possible that Gα subunits activate p115 in part by relieving autoinhibition imposed by the linker region...
  4. ncbi Recognition of the activated states of Galpha13 by the rgRGS domain of PDZRhoGEF
    Zhe Chen
    Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Structure 16:1532-43. 2008
    ..Replacement of key residues in this motif with their counterparts in p115RhoGEF confers GAP activity...
  5. ncbi Structure of the p115RhoGEF rgRGS domain-Galpha13/i1 chimera complex suggests convergent evolution of a GTPase activator
    Zhe Chen
    Department of Biochemistry, The University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390, USA
    Nat Struct Mol Biol 12:191-7. 2005
    ..The interface between the RGS module of rgRGS and Galpha is similar to that of a Galpha-effector complex, suggesting a role for the rgRGS domain in the stimulation of the GEF activity of p115RhoGEF by Galpha13...
  6. ncbi Mapping the Galpha13 binding interface of the rgRGS domain of p115RhoGEF
    Zhe Chen
    Department of Biochemistry, The University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    J Biol Chem 278:9912-9. 2003
    ..This suggests that the rgRGS domain may serve a structural or allosteric role in the regulation of the nucleotide exchange activity of p115RhoGEF on Rho by Galpha(13)...
  7. ncbi Regulation of Rho guanine nucleotide exchange factors by G proteins
    Paul C Sternweis
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Adv Protein Chem 74:189-228. 2007
    ..An overview of identified interactions with other signaling proteins suggests the growing spectrum of their involvement in numerous signaling pathways...
  8. ncbi Structural basis of effector regulation and signal termination in heterotrimeric Galpha proteins
    Stephen R Sprang
    Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Adv Protein Chem 74:1-65. 2007
    ..Unlike GAPs for small GTPases, Galpha GAPs supply no catalytic residues, but rather appear to reduce the activation energy for catalytic activation of the Galpha catalytic site...