Zhe Chen

Summary

Affiliation: University of Texas Southwestern Medical Center
Country: USA

Publications

  1. pmc Activation of p115-RhoGEF requires direct association of Gα13 and the Dbl homology domain
    Zhe Chen
    Department of Biochemistry, The University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390, USA
    J Biol Chem 287:25490-500. 2012
  2. pmc Activated RhoA binds to the pleckstrin homology (PH) domain of PDZ-RhoGEF, a potential site for autoregulation
    Zhe Chen
    Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    J Biol Chem 285:21070-81. 2010
  3. pmc Modulation of a GEF switch: autoinhibition of the intrinsic guanine nucleotide exchange activity of p115-RhoGEF
    Zhe Chen
    Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Protein Sci 20:107-17. 2011
  4. pmc Recognition of the activated states of Galpha13 by the rgRGS domain of PDZRhoGEF
    Zhe Chen
    Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Structure 16:1532-43. 2008
  5. ncbi request reprint Structure of the p115RhoGEF rgRGS domain-Galpha13/i1 chimera complex suggests convergent evolution of a GTPase activator
    Zhe Chen
    Department of Biochemistry, The University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390, USA
    Nat Struct Mol Biol 12:191-7. 2005
  6. pmc Activated RhoA is a positive feedback regulator of the Lbc family of Rho guanine nucleotide exchange factor proteins
    Frank Medina
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    J Biol Chem 288:11325-33. 2013
  7. ncbi request reprint Mapping the Galpha13 binding interface of the rgRGS domain of p115RhoGEF
    Zhe Chen
    Department of Biochemistry, The University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    J Biol Chem 278:9912-9. 2003
  8. ncbi request reprint Regulation of Rho guanine nucleotide exchange factors by G proteins
    Paul C Sternweis
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Adv Protein Chem 74:189-228. 2007
  9. ncbi request reprint Structural basis of effector regulation and signal termination in heterotrimeric Galpha proteins
    Stephen R Sprang
    Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Adv Protein Chem 74:1-65. 2007

Collaborators

  • S R Sprang
  • Xinlin Du
  • Paul C Sternweis
  • Frank Medina
  • Angela M Carter
  • Stephen Gutowski
  • Olugbenga Dada
  • Jana Hadas
  • William D Singer
  • Ying Fan Hsiung
  • Shahab M Danesh

Detail Information

Publications9

  1. pmc Activation of p115-RhoGEF requires direct association of Gα13 and the Dbl homology domain
    Zhe Chen
    Department of Biochemistry, The University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390, USA
    J Biol Chem 287:25490-500. 2012
    ..This study provides new insight into the mechanism of regulation of the RGS-RhoGEF and broadens our understanding of G protein signaling...
  2. pmc Activated RhoA binds to the pleckstrin homology (PH) domain of PDZ-RhoGEF, a potential site for autoregulation
    Zhe Chen
    Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    J Biol Chem 285:21070-81. 2010
    ..PH bound to both forms of RhoA can be isolated by size-exclusion chromatography. This novel interaction between activated RhoA and PH could play a key role in regulation of RhoGEF activity in vivo...
  3. pmc Modulation of a GEF switch: autoinhibition of the intrinsic guanine nucleotide exchange activity of p115-RhoGEF
    Zhe Chen
    Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Protein Sci 20:107-17. 2011
    ..It is possible that Gα subunits activate p115 in part by relieving autoinhibition imposed by the linker region...
  4. pmc Recognition of the activated states of Galpha13 by the rgRGS domain of PDZRhoGEF
    Zhe Chen
    Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Structure 16:1532-43. 2008
    ..Replacement of key residues in this motif with their counterparts in p115RhoGEF confers GAP activity...
  5. ncbi request reprint Structure of the p115RhoGEF rgRGS domain-Galpha13/i1 chimera complex suggests convergent evolution of a GTPase activator
    Zhe Chen
    Department of Biochemistry, The University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390, USA
    Nat Struct Mol Biol 12:191-7. 2005
    ..The interface between the RGS module of rgRGS and Galpha is similar to that of a Galpha-effector complex, suggesting a role for the rgRGS domain in the stimulation of the GEF activity of p115RhoGEF by Galpha13...
  6. pmc Activated RhoA is a positive feedback regulator of the Lbc family of Rho guanine nucleotide exchange factor proteins
    Frank Medina
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    J Biol Chem 288:11325-33. 2013
    ..This mutation is even more dramatic in the context of full-length p115RhoGEF. The utilization of this mechanism by multiple RhoGEFs suggests that this regulatory paradigm may be a common feature in the broader family of RhoGEFs...
  7. ncbi request reprint Mapping the Galpha13 binding interface of the rgRGS domain of p115RhoGEF
    Zhe Chen
    Department of Biochemistry, The University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    J Biol Chem 278:9912-9. 2003
    ..This suggests that the rgRGS domain may serve a structural or allosteric role in the regulation of the nucleotide exchange activity of p115RhoGEF on Rho by Galpha(13)...
  8. ncbi request reprint Regulation of Rho guanine nucleotide exchange factors by G proteins
    Paul C Sternweis
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Adv Protein Chem 74:189-228. 2007
    ..An overview of identified interactions with other signaling proteins suggests the growing spectrum of their involvement in numerous signaling pathways...
  9. ncbi request reprint Structural basis of effector regulation and signal termination in heterotrimeric Galpha proteins
    Stephen R Sprang
    Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Adv Protein Chem 74:1-65. 2007
    ..Unlike GAPs for small GTPases, Galpha GAPs supply no catalytic residues, but rather appear to reduce the activation energy for catalytic activation of the Galpha catalytic site...