Research Topics
| Yan ChenSummaryAffiliation: University of Minnesota Country: USA Publications
Research Grants
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Detail Information
Publications
Dual-color photon counting histogram analysis of mRFP1 and EGFP in living cellsLindsey N Hillesheim
School of Physics and Astronomy, University of Minnesota, Minneapolis, MN 55455, USA
Biophys J 91:4273-84. 2006..We successfully resolved this mixture of fusion proteins with a single dual-color PCH measurement. These results highlight the potential of dual-color PCH to directly detect and quantify protein mixtures in living cells...- Molecular brightness characterization of EGFP in vivo by fluorescence fluctuation spectroscopyYan Chen
Department of Physics, University of Illinois at Urbana Champaign, Urbana, Illinois 61801, USA
Biophys J 82:133-44. 2002..We found the molecular brightness of EGFP to be a very robust parameter, and anticipate the use of PCH analysis for the study of oligomerization processes in vivo... 
Probing nucleocytoplasmic transport by two-photon activation of PA-GFPYan Chen
School of Physics and Astronomy, University of Minnesota, Minneapolis, Minnesota 55455, USA
Microsc Res Tech 69:220-6. 2006..This application demonstrates the attractive features of two-photon photoactivation over traditional techniques, such as photobleaching, for studying transport processes in cells...- Unraveling protein-protein interactions in living cells with fluorescence fluctuation brightness analysisYan Chen
School of Physics and Astronomy, University of Minnesota, Minneapolis, USA
Biophys J 88:4366-77. 2005..We explore binding between these proteins in the presence and absence of RXR agonist. Our results demonstrate that brightness analysis offers a quantitative method for determining protein interactions in cells... - Fluorescence fluctuation spectroscopy on viral-like particles reveals variable gag stoichiometryYan Chen
School of Physics and Astronomy, University of Minnesota, SE Minneapolis, Minnesota 55455, USA
Biophys J 96:1961-9. 2009..This study shows that brightness analysis has the potential to become an important tool for investigating large molecular complexes by providing quantitative information about their size and composition... - Fluorescence fluctuation spectroscopy in the presence of immobile fluorophoresJoseph P Skinner
School of Physics and Astronomy, University of Minnesota, Minneapolis, Minnesota 55455, USA
Biophys J 94:2349-60. 2008..We successfully recovered the brightness of the mobile fraction of H2B-EGFP... - Probing protein oligomerization in living cells with fluorescence fluctuation spectroscopyYan Chen
School of Physics and Astronomy, University of Minnesota, 116 Church Street Southeast, Minneapolis, MN 55455, USA
Proc Natl Acad Sci U S A 100:15492-7. 2003..Resolving protein interactions in a cell is an important step in understanding cellular function on a molecular level. Brightness analysis promises to develop into an important tool for determining protein complex formation in cells... - Molecular brightness determined from a generalized form of Mandel's Q-parameterAlvaro Sanchez-Andres
School of Physics and Astronomy, University of Minnesota, 116 Church Street SE, Minneapolis, MN 55455, USA
Biophys J 89:3531-47. 2005..In addition, we monitor protein association of the ligand-binding domain of retinoid X receptor in the presence and absence of 9-cis-retinoic acid by Q(tau) analysis... - Characterization of cytoplasmic Gag-gag interactions by dual-color z-scan fluorescence fluctuation spectroscopyKeir H Fogarty
Institute for Molecular Virology, University of Minnesota, Minneapolis, Minnesota, USA
Biophys J 100:1587-95. 2011..HIV-1 Gag exhibits concentration-dependent oligomerization in the cytoplasm, whereas HTLV-1 Gag lacks significant cytoplasmic Gag-Gag interactions... - Dual-color time-integrated fluorescence cumulant analysisBin Wu
School of Physics and Astronomy, University of Minnesota, Minneapolis, Minnesota, USA
Biophys J 91:2687-98. 2006..Finally we demonstrate the sensitivity of dual-color TIFCA by resolving EGFP/EYFP binary mixtures in living cells with a single measurement... - Fluorescence fluctuation spectroscopy of mCherry in living cellsBin Wu
School of Physics and Astronomy, University of Minnesota, Minneapolis, Minnesota, USA
Biophys J 96:2391-404. 2009..In addition, we resolved a mixture of interacting and noninteracting proteins labeled with EGFP and mCherry. These results provide the foundation for quantitative applications of mCherry in FFS studies... 
Observing protein interactions and their stoichiometry in living cells by brightness analysis of fluorescence fluctuation experimentsYan Chen
School of Physics and Astronomy, University of Minnesota, Minneapolis, Minnesota, USA
Methods Enzymol 472:345-63. 2010..Protein interactions and stoichiometry are determined from a brightness titration. Experimental details of brightness titration measurements are described together with the necessary calibration and control experiments...- Chromophore maturation and fluorescence fluctuation spectroscopy of fluorescent proteins in a cell-free expression systemPatrick J MacDonald
Department of Biomedical Engineering, University of Minnesota, Minneapolis, MN 55455, USA
Anal Biochem 421:291-8. 2012..We conclude that combining cell-free expression and FFS provides a powerful technique for quick, quantitative study of chromophore maturation and protein-protein interaction... - New insights into HTLV-1 particle structure, assembly, and Gag-Gag interactions in living cellsKeir H Fogarty
Institute for Molecular Virology, University of Minnesota, Minneapolis, Minneapolis, MN 55455, USA
Viruses 3:770-93. 2011..The application of these and other novel biophysical approaches promise to provide exciting new insights into HTLV-1 replication... - Determining the stoichiometry of protein heterocomplexes in living cells with fluorescence fluctuation spectroscopyYan Chen
School of Physics and Astronomy, University of Minnesota, 116 Church Street Southeast, Minneapolis, MN 55455, USA
Proc Natl Acad Sci U S A 104:3147-52. 2007..This study provides proof-of-principle experiments that illustrate the potential of brightness analysis as a powerful tool for quantifying protein interactions in living cells... - Heterospecies partition analysis reveals binding curve and stoichiometry of protein interactions in living cellsBin Wu
School of Physics and Astronomy, University of Minnesota, Minneapolis, MN 55455, USA
Proc Natl Acad Sci U S A 107:4117-22. 2010.... - Cellular characterization of adenylate kinase and its isoform: two-photon excitation fluorescence imaging and fluorescence correlation spectroscopyQiaoqiao Ruan
Laboratory for Fluorescence Dynamics, Department, University of Illinois in Urbana-Champaign, 1110 W. Green Street, Urbana, IL 61801, USA
Biophys J 83:3177-87. 2002..This technology offers advantages in studying protein interactions and function in the cell... - Brightness analysis by Z-scan fluorescence fluctuation spectroscopy for the study of protein interactions within living cellsPatrick J MacDonald
School of Physics and Astronomy, University of Minnesota, Minneapolis, Minnesota, USA
Biophys J 99:979-88. 2010..We demonstrate that z-scan FFS abolishes the brightness artifact and use the method to determine the oligomerization of cytoplasmic nuclear transport factor 2... - Purification of prenylated proteins by affinity chromatography on cyclodextrin-modified agaroseJinhwa A Chung
Department of Chemistry, University of Minnesota, Minneapolis, 55455, USA
Anal Biochem 386:1-8. 2009..We propose that this method could be generally useful to the community of researchers studying protein prenylation... - Characterization of brightness and stoichiometry of bright particles by flow-fluorescence fluctuation spectroscopyJolene Johnson
School of Physics and Astronomy, University of Minnesota, Minneapolis, Minnesota, USA
Biophys J 99:3084-92. 2010..Our results demonstrate that flow-FFS is a sensitive method for the characterization of complex macromolecular particles at low concentrations... - Biophysical analysis of HTLV-1 particles reveals novel insights into particle morphology and Gag stochiometryIwen F Grigsby
Institute for Molecular Virology, University of Minnesota, Minneapolis, MN 55455, USA
Retrovirology 7:75. 2010..In this study, we created a codon-optimized HTLV-1 Gag fused to an EYFP reporter as a model system to quantitatively analyze HTLV-1 particles released from producer cells... - Position-sensitive scanning fluorescence correlation spectroscopyJoseph P Skinner
School of Physics and Astronomy, University of Minnesota, Minneapolis, 55455, USA
Biophys J 89:1288-301. 2005..To demonstrate the feasibility of the technique for applications in living cells we present data of enhanced green fluorescent protein measured in the nucleus of COS cells... - Fluorescence correlation spectroscopyJoachim D Müller
Department of Physics and Astronomy, University of Minnesota, Minneapolis, Minnesota 55455, USA
Methods Enzymol 361:69-92. 2003 - Fluorescence correlation spectroscopy of finite-sized particlesBin Wu
School of Physics and Astronomy, University of Minnesota, Minneapolis, Minnesota 55455, USA
Biophys J 94:2800-8. 2008..In addition, experiments are performed on fluorescent spheres of different radii to validate the model by comparing the results to theoretical predictions... - Molecular brightness analysis reveals phosphatidylinositol 4-Kinase IIβ association with clathrin-coated vesicles in living cellsJinhui Li
Physics Department, University of Minnesota, Minneapolis, Minnesota, USA
Biophys J 103:1657-65. 2012..Moreover, we establish molecular brightness as a powerful tool for characterizing cellular cytosolic vesicles that are otherwise difficult to characterize by other techniques... - Brightness analysisPatrick Macdonald
Department of Biomedical Engineering, University of Minnesota, Minneapolis, Minnesota, USA
Methods Enzymol 518:71-98. 2013..Brightness measurements in thin sample geometries, frequently encountered in cells, may be biased. We use z-scan FFS to characterize sample geometry and correct any resulting bias in the brightness... - A universal method for the preparation of covalent protein-DNA conjugates for use in creating protein nanostructuresBenjamin P Duckworth
Department of Chemistry, University of Minnesota, Minneapolis, MN 55455, USA
Angew Chem Int Ed Engl 46:8819-22. 2007 - Dual-color photon-counting histogramYan Chen
School of Physics and Astronomy, University of Minnesota, 116 Church St, SE Minneapolis, MN 55455, USA
Biophys J 88:2177-92. 2005..We quantify the influence of these filters on dual-color PCH analysis and determine the optimal transition wavelength of the dichroic mirror for the CFP-YFP pair... - Smad3 mediates activin-induced transcription of follicle-stimulating hormone beta-subunit geneMagdalena I Suszko
Department of Neurobiology and Physiology, Northwestern University, 2205 Tech Drive, Evanston, IL 60208 USA
Mol Endocrinol 19:1849-58. 2005.... - Latent transforming growth factor beta-binding protein 1 interacts with fibrillin and is a microfibril-associated proteinZenzo Isogai
Department of Biochemistry and Molecular Biology, Shriners Hospital for Children, Oregon Health and Science University, Portland 97239, USA
J Biol Chem 278:2750-7. 2003..In tissues where LTBP-1 is not expressed, LTBP-4 may substitute for LTBP-1, because the C-terminal end of LTBP-4 binds equally well to fibrillin. A model depicting the relationship between LTBP-1 and fibrillin microfibrils is proposed... - Application of Multivariate curve resolution-alternating least square methods on the resolution of overlapping CE peaks from different separation conditionsFang Zhang
Institute of Analytical Science, Northwest University, Xi an, People s Republic of China
Electrophoresis 28:3674-83. 2007.... - Characterization of a Bcl-XL-interacting protein FKBP8 and its splice variant in human RPE cellsYan Chen
Vanderbilt Eye Institute, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA
Invest Ophthalmol Vis Sci 49:1721-7. 2008..The purpose of this study was to define the expression of the FKBP8 gene in cultured human RPE cells and explore its involvement in the control of apoptosis... - Latent TGF-beta binding protein-3 (LTBP-3) requires binding to TGF-beta for secretionYan Chen
Department of Cell Biology, New York University School of Medicine, New York, NY 10016, USA
FEBS Lett 517:277-80. 2002..The secretion of LTBP-3 requires complexing of LTBP-3 with Cys33 of the TGF-beta propeptide... - Overexpressed human mitochondrial thioredoxin confers resistance to oxidant-induced apoptosis in human osteosarcoma cellsYan Chen
Program of Biochemistry, Cell, and Developmental Biology, Emory University, Atlanta, Georgia 30322, USA
J Biol Chem 277:33242-8. 2002..Thus, human mtTrx is a member of the thioredoxin family of proteins localized to mitochondria and may play important roles in protection against oxidant-induced apoptosis... - Phosphorylation regulation of the interaction between Smad7 and activin type I receptorXubao Liu
Department of Medical and Molecular Genetics and the Walther Oncology Center, Indiana University School of Medicine, and the Walther Cancer Institute, 975 West Walnut Street, IB130, Indianapolis, IN 46202, USA
FEBS Lett 519:93-8. 2002..These studies not only illustrated the counter regulatory function of Smad7 on activin signaling, but also indicated the involvement of phosphorylation at activin type I receptor in the inhibitory action of Smad7... - Amino acid requirements for formation of the TGF-beta-latent TGF-beta binding protein complexesYan Chen
Department of Cell Biology, New York University School of Medicine, 550 First Avenue, New York, NY 10016, USA
J Mol Biol 345:175-86. 2005.... - Fibronectin is required for integrin alphavbeta6-mediated activation of latent TGF-beta complexes containing LTBP-1Laura Fontana
Department of Cell Biology, New York University School of Medicine, New York, NY 10016, USA
FASEB J 19:1798-808. 2005..The results indicate specificity for integrin-mediated latent TGF-beta activation that include unique sequences in LTBP-1 and an appropriate matrix molecule... - Domain mapping studies reveal that the M domain of hsp90 serves as a molecular scaffold to regulate Akt-dependent phosphorylation of endothelial nitric oxide synthase and NO releaseJason Fontana
Department of Pharmacology and Molecular Cardiobiology Program, Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, Conn 06536, USA
Circ Res 90:866-73. 2002.... - [Effect of Epstein-Barr virus latent membrane protein 1 (LMP1) on apoptosis of nasopharyngeal carcinoma cell line CNE1]Yan Chen
Department of Pathology, Guangdong Medical College, Zhanjiang 524023, P. R. China
Ai Zheng 21:498-503. 2002..CONCLUSIONS: The LMP1 gene was transfected successfully into CNE1 cells which were stably expressing LMP1. LMP1 could increase apoptosis occurring of CNE1 cells in the process of inducing apoptosis... - Using magnetic force to enhance immune response to DNA vaccineXian-Feng Zhou
College of Life Science, Jilin University, Huoju Road 1260, Changchun 130012, PR China
Small 3:1707-13. 2007 - Endostar suppresses invasion through downregulating the expression of matrix metalloproteinase-2/9 in MDA-MB-435 human breast cancer cellsNa Lu
Jiangsu Key Laboratory of Carcinogenesis and Intervention, China Pharmaceutical University, 210009 Nanjing, People s Republic of China
Exp Biol Med (Maywood) 233:1013-20. 2008..Additionally, endostar exerted an inhibitory effect on the phosphorylation of ERK1/2. Collectively, these data provided a molecular basis for the anti-invasive effects of endostar... - A highly specific BODIPY-based fluorescent probe for the detection of hypochlorous acidZhen Ning Sun
Department of Chemistry, The University of Hong Kong, Hong Kong, P R China
Org Lett 10:2171-4. 2008..This new probe might be used as an efficient tool for probing the roles HOCl plays in biological systems... - [Interleukin-12 enhanced tumor necrosis factor related apoptosis-inducing ligand TRAIL-induced apoptosis in human hepatocellular carcinoma by inhibiting expression of survivin]Song-qing He
Hepatic Surgery Center, Affiliated Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
Zhonghua Wai Ke Za Zhi 41:453-7. 2003..IL-12 may sensitize HCC cells to TRAIL-induced apoptosis by preventing survivin. Combining gene therapy strategy such as combining gene therapy of TRAIL with IL-12 may be a promising maneuver to HCC... - Designing chitosan-dextran sulfate nanoparticles using charge ratiosYan Chen
School of Pharmacy, Curtin University of Technology, GPO Box U1987, Perth, Western Australia
AAPS PharmSciTech 8:E98. 2007..The CS-DS nanoparticles developed based on the modulation of charge ratio show promise as a system for controlled delivery of both small and large molecules, including proteins... - RKTG sequesters B-Raf to the Golgi apparatus and inhibits the proliferation and tumorigenicity of human malignant melanoma cellsFengjuan Fan
Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031, China
Carcinogenesis 29:1157-63. 2008..Collectively, our results suggest that RKTG may play a suppressive role in human melanoma that harbors an oncogenic B-Raf mutation via its antagonistic action on B-Raf... - Characterization of the topology and functional domains of RKTGXiaolin Luo
Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Graduate School of the Chinese Academy of Sciences, 294 Taiyuan Road, Shanghai 200031, China
Biochem J 414:399-406. 2008.... - Transcriptional regulation of tristetraprolin by transforming growth factor-beta in human T cellsKenji Ogawa
Department of Medical and Molecular Genetics, Walther Oncology Center, Indiana University School of Medicine and the Walther Cancer Institute, Indianapolis, Indiana 46202, USA
J Biol Chem 278:30373-81. 2003.... - A deterministic iterative least-squares algorithm for beam weight optimization in conformal radiotherapyYan Chen
Department of Radiation Oncology, Kimmel Cancer Center, Jefferson Medical College of Thomas Jefferson University, Philadelphia, PA 19107, USA
Phys Med Biol 47:1647-58. 2002..The method is robust in solving difficult clinical cases using either aperture or pencil beam based planning techniques for intensity-modulated radiation therapy... - The cyclopentenone product of lipid peroxidation, 15-A(2t)-isoprostane (8-isoprostaglandin A(2)), is efficiently conjugated with glutathione by human and rat glutathione transferase A4-4Ina Hubatsch
Department of Biochemistry, Uppsala University, SE-75123 Uppsala, Sweden
Chem Res Toxicol 15:1114-8. 2002..These studies have therefore defined a potentially important route by which cyclopentenone IsoPs are metabolized that may serve as a mechanism for the inactivation of these highly reactive compounds... - [Expression of histone-based fusion protein HNHG in E.coli]Fei Han Dai
State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai 200032, China
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 34:800-3. 2002..This specific ability of the fusion protein HNHG of binding and condensing plasmids could be utilized to construct novel exogenous gene delivery systems. HNHG would be a promising candidate for gene delivery... - Large-scale cDNA transfection screening for genes related to cancer development and progressionDafang Wan
National Laboratory for Oncogenes and Related Genes, Cancer Institute of Shanghai Jiao Tong University, 25/Ln 2200 Xie-Tu Road, Shanghai 200032, People's Republic of China
Proc Natl Acad Sci U S A 101:15724-9. 2004..It offers a better understanding of the basic machinery of oncogenesis, including previously undescribed systemic regulatory mechanisms, and also provides a tool for gene discovery with potential clinical applications... - [Apoptosis of Fas(+) Yac-1 cells induced with Fas ligand-transfected COS-7 cells]Ling-Bo Liu
Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
Zhongguo Shi Yan Xue Ye Xue Za Zhi 10:327-31. 2002..01), in which the COS-7 cell was transfected by pBillneo (not carrying mFasL gene). It was concluded that lymphoma cells highly expressing Fas can be effectively killed through single Fas-FasL way in vitro... - An optimization algorithm for intensity modulated radiotherapy--the simulated dynamics with dose-volume constraintsQing Hou
Key Lab for Radiation Physics and Technology, Institute of Nuclear Science and Technology, Sichuan University, Chengdu 610064, China
Med Phys 30:61-8. 2003..In addition to the convenience of implementation, our method can achieve a high computational efficiency with the understanding of the dynamic behavior of the system... - Using aptamers to study protein-protein interactionsParag Parekh
Department of Chemistry and Department of Physiology and Functional Genomics, Shands Cancer Center, Center for Research at the Bio Nano Interface, University of Florida, Gainesville, FL 32611 7200, USA
Adv Biochem Eng Biotechnol 110:177-94. 2008..To accomplish this objective, we report the use of DNA/RNA aptamers as a novel tool for the study and elucidation of protein-protein interactions, both in vivo and in vitro... - Light-switching excimer beacon assays for ribonuclease H kinetic studyYan Chen
Center for Research at the Bio/Nano Interface, Department of Chemistry, UF Genetics Institute, Shands Cancer Center and McKnight Brain Institute, University of Florida, Gainesville, FL 32611-7200, USA
Chembiochem 9:355-9. 2008 - Beam orientation optimization for IMRT by a hybrid method of the genetic algorithm and the simulated dynamicsQing Hou
Key Lab for Radiation Physics and Technology, Institute of Nuclear Science and Technology, Sichuan University, Chengdu 610064, China
Med Phys 30:2360-7. 2003..g., the smoothness of intensity profiles, for the selection of the optimal plan among the degenerate configurations for treatment delivery... - A molecular phylogeny of eurytomid wasps inferred from DNA sequence data of 28S, 18S, 16S, and COI genesYan Chen
Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China
Mol Phylogenet Evol 31:300-7. 2004..Subsequent taxonomic revisions include elevating the subfamily Rileyinae to a family status and the divisions of the genera Eurytoma and Bruchophagus... - Atrial natriuretic peptide promotes cardiomyocyte survival by cGMP-dependent nuclear accumulation of zyxin and AktTakahiro Kato
San Diego State University Heart Institute, Department of Biology, San Diego, California 92182, USA
J Clin Invest 115:2716-30. 2005..Nuclear accumulation of zyxin and activated Akt may represent a fundamental mechanism that facilitates nuclear-signal transduction and potentiates cell survival... - Comparison of diafiltration and tangential flow filtration for purification of nanoparticle suspensionsGautam Dalwadi
Western Australian Biomedical Research Institute, School of Pharmacy, Curtin University of Technology, Perth, Australia
Pharm Res 22:2152-62. 2005..The DCD technique can be an alternative purification method for nanoparticle dispersions of small volumes... - Suppression of matrix metalloproteinase-9 transcription by transforming growth factor-beta is mediated by a nuclear factor-kappaB siteKenji Ogawa
Department of Medical and Molecular Genetics, Walther Oncology Center, Indiana University School of Medicine and the Walther Cancer Institute, Indianapolis, IN 46202, USA
Biochem J 381:413-22. 2004..Taken together, these studies indicate that the NF-kappaB site is indispensable for the suppressive activity of TGF-beta in the regulation of MMP-9 transcription... - Spatiotemporal pattern of calmodulin and [Ca2+]i is related to resumption of meiosis in mouse oocytesChun-Ming Bi
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China
Cell Biol Int 28:317-22. 2004..Taking the data from calcium chelation and CaM inhibition together, our results suggest that the dynamic distribution patterns of calcium and activated CaM are crucial for oocyte maturation... - [Construction and expression of eukaryotic expression vector containing hVEGF165 gene in rat bone marrow stroma cell]Quan wen Gao
Department of Oral Histology and Pathology, College of Stomatology, Fourth Military Medical University, Xi an 710032, China
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 19:32-4. 2003..To construct the eukaryotic expression vector containing human vascular endothelial growth factor 165(VEGF165) gene and express it in rat bone marrow stroma cells(rMSCs)... - The association of calmodulin with central spindle regulates the initiation of cytokinesis in HeLa cellsYue Yue Yu
Jiangsu Key Laboratory for Molecular and Medical Biotechnology, School of Life Sciences, Nanjing Normal University, Nanjing 210097, Jiangsu Province, China
Int J Biochem Cell Biol 36:1562-72. 2004.... - Characterization of HPV16 L1 loop domains in the formation of a type-specific, conformational epitopeVanessa A Olcese
Department of Pathology, Georgetown University Medical Center, Washington, DC 20057, USA
BMC Microbiol 4:29. 2004.... - Surface amplification of invasive cleavage productsYan Chen
Department of Chemistry, University of Wisconsin-Madison, 1101 University Avenue, Madison, Wisconsin 53706-1396, USA
J Am Chem Soc 126:3016-7. 2004..This combination of very specific surface cleavage and highly sensitive surface detection will make possible the rapid and parallel analysis of genetic variations across large populations... - IDGraphs: intrusion detection and analysis using stream compositingPin Ren
Department of Electrical Engineering and Computer Science, Northwestern University, USA
IEEE Comput Graph Appl 26:28-39. 2006 - Antagonists to human and mouse vascular endothelial growth factor receptor 2 generated by directed protein evolution in vitroElena V Getmanova
Phylos, Inc, succeeded by Compound Therapeutics, Inc, 100 Beaver Street, Waltham, Massachusetts 02453, USA
Chem Biol 13:549-56. 2006..Proteins encoded by the selected clones bound VEGFR2-expressing cells and inhibited their VEGF-dependent proliferation. Our results demonstrate the potential of these inhibitors in the development of anti-angiogenesis therapeutics... - Tumor-derived C-terminal mutations of Smad4 with decreased DNA binding activity and enhanced intramolecular interactionChenzhong Kuang
Department of Medical and Molecular Genetics, Indiana University School of Medicine and the Walther Cancer Institute, Indianapolis, IN 46202, USA
Oncogene 23:1021-9. 2004..Therefore, these studies revealed novel mechanisms by which the Smad4 mutations utilize to abrogate their functions in transducing the signaling of TGF-beta, which plays an important role in various stages of cancer formation... - The key virulence-associated genes of Streptococcus suis type 2 are upregulated and differentially expressed in vivoChen Tan
College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
FEMS Microbiol Lett 278:108-14. 2008..Characterization of the differential expression patterns of this subset of key virulence-associated genes facilitates further study into the importance of these genes in S. suis serotype 2 pathogenesis in pigs... - Controlled release of PEI/DNA complexes from PLGA microspheres as a potent delivery system to enhance immune response to HIV vaccine DNA prime/MVA boost regimeXianfeng Zhou
College of Life Science, Jilin University, Changchun, PR China
Eur J Pharm Biopharm 68:589-95. 2008..m. immunization, the microspheres induced significantly enhanced serum antibody responses 2-3 orders of magnitude greater than naked DNA. Additionally, in contrast to naked DNA, the microspheres induced potent CTL responses at a low dose... - Enhance immune response to DNA vaccine based on a novel multicomponent supramolecular assemblyXianfeng Zhou
College of Life Science, Key Laboratory for Supramolecular Structure and Materials of Ministry of Education, Jilin University, Changchun 130012, and Sichuan Tumor Hospital and Institute, Chengdu, PR China
Biomaterials 28:4684-92. 2007..Consequently, formulation of DNA vaccines into multicomponent vectors is a powerful means of increasing vaccine potency... - [Transduction efficiency of recombinant adeno-associated virus 2 in human umbilical cord blood CD34+ hematopoietic stem/progenitor cells]Yan Chen
Department of Hematology, Xiangya Hospital of Central South University, Changsha 410008, China
Zhongguo Shi Yan Xue Ye Xue Za Zhi 11:576-8. 2003..The results showed that 43% CD34(+) cells expressed the GFP gene at a multiplicity of infection of 2 x 10(5). It is concluded that the rAAV-2 can transduce human cord blood CD34(+) hematopoietic stem/progenitor cells efficiently... - Activated c-Fms recruits Vav and Rac during CSF-1-induced cytoskeletal remodeling and spreading in osteoclastsHiroaki Sakai
Yale School of Medicine, TAC S 133, PO Box 208020, New Haven, CT 06520 8020, USA
Bone 39:1290-301. 2006..These data demonstrate that Vav and Rac lie downstream from activated PI3-K in CSF-1-treated osteoclasts and that Rac is required for CSF-1-induced cytoskeletal remodeling in these cells... - Augmenter of liver regeneration promotes hepatocyte proliferation induced by Kupffer cellsChun Ping Wang
Department of Gastroenterology, 302 Hospital of Chinese PLA, 100 Xisihuan Middle Road, Beijing 100039, China
World J Gastroenterol 12:4859-65. 2006..To observe the effects of augmenter of liver regeneration (ALR) on Kupffer cells and to determine whether ALR promotes hepatocyte proliferation induced by Kupffer cells... - Functional silencing of hepatic microsomal glucose-6-phosphatase gene expression in vivo by adenovirus-mediated delivery of short hairpin RNAAlan Huang
Millennium Pharmaceuticals, 75 Sidney St, Cambridge, MA 02139, USA
FEBS Lett 558:69-73. 2004..These findings provide evidence that AdV delivery of expressed shRNA can be a productive tool to explore gene function in vivo... - Sequences of B-chain/domain 1-10/1-9 of insulin and insulin-like growth factor 1 determine their different folding behaviorYan Chen
Key Laboratory of Proteomics, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Graduate School, The Chinese Academy of Sciences, Shanghai 200031, China
Biochemistry 43:9225-33. 2004....
Research Grants
- In Vivo fluorescence correlation spectroscopyYan Chen; Fiscal Year: 2003..In addition, GFP tagged retinoid receptors will be used to perform an in vivo study to resolve their molecular oligomerization state in the presence and absence of ligands. ..