Yan Chen

Summary

Affiliation: University of Minnesota
Country: USA

Publications

  1. pmc Dual-color photon counting histogram analysis of mRFP1 and EGFP in living cells
    Lindsey N Hillesheim
    School of Physics and Astronomy, University of Minnesota, Minneapolis, MN 55455, USA
    Biophys J 91:4273-84. 2006
  2. pmc Molecular brightness characterization of EGFP in vivo by fluorescence fluctuation spectroscopy
    Yan Chen
    Department of Physics, University of Illinois at Urbana Champaign, Urbana, Illinois 61801, USA
    Biophys J 82:133-44. 2002
  3. ncbi request reprint Probing nucleocytoplasmic transport by two-photon activation of PA-GFP
    Yan Chen
    School of Physics and Astronomy, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Microsc Res Tech 69:220-6. 2006
  4. pmc Unraveling protein-protein interactions in living cells with fluorescence fluctuation brightness analysis
    Yan Chen
    School of Physics and Astronomy, University of Minnesota, Minneapolis, USA
    Biophys J 88:4366-77. 2005
  5. pmc Fluorescence fluctuation spectroscopy on viral-like particles reveals variable gag stoichiometry
    Yan Chen
    School of Physics and Astronomy, University of Minnesota, SE Minneapolis, Minnesota 55455, USA
    Biophys J 96:1961-9. 2009
  6. pmc Probing protein oligomerization in living cells with fluorescence fluctuation spectroscopy
    Yan Chen
    School of Physics and Astronomy, University of Minnesota, 116 Church Street Southeast, Minneapolis, MN 55455, USA
    Proc Natl Acad Sci U S A 100:15492-7. 2003
  7. pmc Fluorescence fluctuation spectroscopy in the presence of immobile fluorophores
    Joseph P Skinner
    School of Physics and Astronomy, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Biophys J 94:2349-60. 2008
  8. pmc Characterization of cytoplasmic Gag-gag interactions by dual-color z-scan fluorescence fluctuation spectroscopy
    Keir H Fogarty
    Institute for Molecular Virology, University of Minnesota, Minneapolis, Minnesota, USA
    Biophys J 100:1587-95. 2011
  9. pmc Molecular brightness determined from a generalized form of Mandel's Q-parameter
    Alvaro Sanchez-Andres
    School of Physics and Astronomy, University of Minnesota, 116 Church Street SE, Minneapolis, MN 55455, USA
    Biophys J 89:3531-47. 2005
  10. pmc Fluorescence fluctuation spectroscopy of mCherry in living cells
    Bin Wu
    School of Physics and Astronomy, University of Minnesota, Minneapolis, Minnesota, USA
    Biophys J 96:2391-404. 2009

Research Grants

Collaborators

Detail Information

Publications76

  1. pmc Dual-color photon counting histogram analysis of mRFP1 and EGFP in living cells
    Lindsey N Hillesheim
    School of Physics and Astronomy, University of Minnesota, Minneapolis, MN 55455, USA
    Biophys J 91:4273-84. 2006
    ..We successfully resolved this mixture of fusion proteins with a single dual-color PCH measurement. These results highlight the potential of dual-color PCH to directly detect and quantify protein mixtures in living cells...
  2. pmc Molecular brightness characterization of EGFP in vivo by fluorescence fluctuation spectroscopy
    Yan Chen
    Department of Physics, University of Illinois at Urbana Champaign, Urbana, Illinois 61801, USA
    Biophys J 82:133-44. 2002
    ..We found the molecular brightness of EGFP to be a very robust parameter, and anticipate the use of PCH analysis for the study of oligomerization processes in vivo...
  3. ncbi request reprint Probing nucleocytoplasmic transport by two-photon activation of PA-GFP
    Yan Chen
    School of Physics and Astronomy, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Microsc Res Tech 69:220-6. 2006
    ..This application demonstrates the attractive features of two-photon photoactivation over traditional techniques, such as photobleaching, for studying transport processes in cells...
  4. pmc Unraveling protein-protein interactions in living cells with fluorescence fluctuation brightness analysis
    Yan Chen
    School of Physics and Astronomy, University of Minnesota, Minneapolis, USA
    Biophys J 88:4366-77. 2005
    ..We explore binding between these proteins in the presence and absence of RXR agonist. Our results demonstrate that brightness analysis offers a quantitative method for determining protein interactions in cells...
  5. pmc Fluorescence fluctuation spectroscopy on viral-like particles reveals variable gag stoichiometry
    Yan Chen
    School of Physics and Astronomy, University of Minnesota, SE Minneapolis, Minnesota 55455, USA
    Biophys J 96:1961-9. 2009
    ..This study shows that brightness analysis has the potential to become an important tool for investigating large molecular complexes by providing quantitative information about their size and composition...
  6. pmc Probing protein oligomerization in living cells with fluorescence fluctuation spectroscopy
    Yan Chen
    School of Physics and Astronomy, University of Minnesota, 116 Church Street Southeast, Minneapolis, MN 55455, USA
    Proc Natl Acad Sci U S A 100:15492-7. 2003
    ..Resolving protein interactions in a cell is an important step in understanding cellular function on a molecular level. Brightness analysis promises to develop into an important tool for determining protein complex formation in cells...
  7. pmc Fluorescence fluctuation spectroscopy in the presence of immobile fluorophores
    Joseph P Skinner
    School of Physics and Astronomy, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Biophys J 94:2349-60. 2008
    ..We successfully recovered the brightness of the mobile fraction of H2B-EGFP...
  8. pmc Characterization of cytoplasmic Gag-gag interactions by dual-color z-scan fluorescence fluctuation spectroscopy
    Keir H Fogarty
    Institute for Molecular Virology, University of Minnesota, Minneapolis, Minnesota, USA
    Biophys J 100:1587-95. 2011
    ..HIV-1 Gag exhibits concentration-dependent oligomerization in the cytoplasm, whereas HTLV-1 Gag lacks significant cytoplasmic Gag-Gag interactions...
  9. pmc Molecular brightness determined from a generalized form of Mandel's Q-parameter
    Alvaro Sanchez-Andres
    School of Physics and Astronomy, University of Minnesota, 116 Church Street SE, Minneapolis, MN 55455, USA
    Biophys J 89:3531-47. 2005
    ..In addition, we monitor protein association of the ligand-binding domain of retinoid X receptor in the presence and absence of 9-cis-retinoic acid by Q(tau) analysis...
  10. pmc Fluorescence fluctuation spectroscopy of mCherry in living cells
    Bin Wu
    School of Physics and Astronomy, University of Minnesota, Minneapolis, Minnesota, USA
    Biophys J 96:2391-404. 2009
    ..In addition, we resolved a mixture of interacting and noninteracting proteins labeled with EGFP and mCherry. These results provide the foundation for quantitative applications of mCherry in FFS studies...
  11. pmc Dual-color time-integrated fluorescence cumulant analysis
    Bin Wu
    School of Physics and Astronomy, University of Minnesota, Minneapolis, Minnesota, USA
    Biophys J 91:2687-98. 2006
    ..Finally we demonstrate the sensitivity of dual-color TIFCA by resolving EGFP/EYFP binary mixtures in living cells with a single measurement...
  12. doi request reprint Observing protein interactions and their stoichiometry in living cells by brightness analysis of fluorescence fluctuation experiments
    Yan Chen
    School of Physics and Astronomy, University of Minnesota, Minneapolis, Minnesota, USA
    Methods Enzymol 472:345-63. 2010
    ..Protein interactions and stoichiometry are determined from a brightness titration. Experimental details of brightness titration measurements are described together with the necessary calibration and control experiments...
  13. pmc Chromophore maturation and fluorescence fluctuation spectroscopy of fluorescent proteins in a cell-free expression system
    Patrick J MacDonald
    Department of Biomedical Engineering, University of Minnesota, Minneapolis, MN 55455, USA
    Anal Biochem 421:291-8. 2012
    ..We conclude that combining cell-free expression and FFS provides a powerful technique for quick, quantitative study of chromophore maturation and protein-protein interaction...
  14. pmc New insights into HTLV-1 particle structure, assembly, and Gag-Gag interactions in living cells
    Keir H Fogarty
    Institute for Molecular Virology, University of Minnesota, Minneapolis, Minneapolis, MN 55455, USA
    Viruses 3:770-93. 2011
    ..The application of these and other novel biophysical approaches promise to provide exciting new insights into HTLV-1 replication...
  15. pmc Cellular characterization of adenylate kinase and its isoform: two-photon excitation fluorescence imaging and fluorescence correlation spectroscopy
    Qiaoqiao Ruan
    Laboratory for Fluorescence Dynamics, Department, University of Illinois in Urbana Champaign, 1110 W Green Street, Urbana, IL 61801, USA
    Biophys J 83:3177-87. 2002
    ..This technology offers advantages in studying protein interactions and function in the cell...
  16. pmc Determining the stoichiometry of protein heterocomplexes in living cells with fluorescence fluctuation spectroscopy
    Yan Chen
    School of Physics and Astronomy, University of Minnesota, 116 Church Street Southeast, Minneapolis, MN 55455, USA
    Proc Natl Acad Sci U S A 104:3147-52. 2007
    ..This study provides proof-of-principle experiments that illustrate the potential of brightness analysis as a powerful tool for quantifying protein interactions in living cells...
  17. pmc Heterospecies partition analysis reveals binding curve and stoichiometry of protein interactions in living cells
    Bin Wu
    School of Physics and Astronomy, University of Minnesota, Minneapolis, MN 55455, USA
    Proc Natl Acad Sci U S A 107:4117-22. 2010
    ....
  18. pmc Brightness analysis by Z-scan fluorescence fluctuation spectroscopy for the study of protein interactions within living cells
    Patrick J MacDonald
    School of Physics and Astronomy, University of Minnesota, Minneapolis, Minnesota, USA
    Biophys J 99:979-88. 2010
    ..We demonstrate that z-scan FFS abolishes the brightness artifact and use the method to determine the oligomerization of cytoplasmic nuclear transport factor 2...
  19. pmc Purification of prenylated proteins by affinity chromatography on cyclodextrin-modified agarose
    Jinhwa A Chung
    Department of Chemistry, University of Minnesota, Minneapolis, 55455, USA
    Anal Biochem 386:1-8. 2009
    ..We propose that this method could be generally useful to the community of researchers studying protein prenylation...
  20. pmc Characterization of brightness and stoichiometry of bright particles by flow-fluorescence fluctuation spectroscopy
    Jolene Johnson
    School of Physics and Astronomy, University of Minnesota, Minneapolis, Minnesota, USA
    Biophys J 99:3084-92. 2010
    ..Our results demonstrate that flow-FFS is a sensitive method for the characterization of complex macromolecular particles at low concentrations...
  21. doi request reprint Brightness analysis
    Patrick Macdonald
    Department of Biomedical Engineering, University of Minnesota, Minneapolis, Minnesota, USA
    Methods Enzymol 518:71-98. 2013
    ..Brightness measurements in thin sample geometries, frequently encountered in cells, may be biased. We use z-scan FFS to characterize sample geometry and correct any resulting bias in the brightness...
  22. pmc Biophysical analysis of HTLV-1 particles reveals novel insights into particle morphology and Gag stochiometry
    Iwen F Grigsby
    Institute for Molecular Virology, University of Minnesota, Minneapolis, MN 55455, USA
    Retrovirology 7:75. 2010
    ..In this study, we created a codon-optimized HTLV-1 Gag fused to an EYFP reporter as a model system to quantitatively analyze HTLV-1 particles released from producer cells...
  23. pmc Position-sensitive scanning fluorescence correlation spectroscopy
    Joseph P Skinner
    School of Physics and Astronomy, University of Minnesota, Minneapolis, 55455, USA
    Biophys J 89:1288-301. 2005
    ..To demonstrate the feasibility of the technique for applications in living cells we present data of enhanced green fluorescent protein measured in the nucleus of COS cells...
  24. ncbi request reprint Fluorescence correlation spectroscopy
    Joachim D Müller
    Department of Physics and Astronomy, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Methods Enzymol 361:69-92. 2003
  25. pmc Fluorescence correlation spectroscopy of finite-sized particles
    Bin Wu
    School of Physics and Astronomy, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Biophys J 94:2800-8. 2008
    ..In addition, experiments are performed on fluorescent spheres of different radii to validate the model by comparing the results to theoretical predictions...
  26. pmc Molecular brightness analysis reveals phosphatidylinositol 4-Kinase IIβ association with clathrin-coated vesicles in living cells
    Jinhui Li
    Physics Department, University of Minnesota, Minneapolis, Minnesota, USA
    Biophys J 103:1657-65. 2012
    ..Moreover, we establish molecular brightness as a powerful tool for characterizing cellular cytosolic vesicles that are otherwise difficult to characterize by other techniques...
  27. pmc Dual-color photon-counting histogram
    Yan Chen
    School of Physics and Astronomy, University of Minnesota, 116 Church St, SE Minneapolis, MN 55455, USA
    Biophys J 88:2177-92. 2005
    ..We quantify the influence of these filters on dual-color PCH analysis and determine the optimal transition wavelength of the dichroic mirror for the CFP-YFP pair...
  28. ncbi request reprint A universal method for the preparation of covalent protein-DNA conjugates for use in creating protein nanostructures
    Benjamin P Duckworth
    Department of Chemistry, University of Minnesota, Minneapolis, MN 55455, USA
    Angew Chem Int Ed Engl 46:8819-22. 2007
  29. ncbi request reprint Domain mapping studies reveal that the M domain of hsp90 serves as a molecular scaffold to regulate Akt-dependent phosphorylation of endothelial nitric oxide synthase and NO release
    Jason Fontana
    Department of Pharmacology and Molecular Cardiobiology Program, Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, Conn 06536, USA
    Circ Res 90:866-73. 2002
    ....
  30. ncbi request reprint Phosphorylation regulation of the interaction between Smad7 and activin type I receptor
    Xubao Liu
    Department of Medical and Molecular Genetics and the Walther Oncology Center, Indiana University School of Medicine, and the Walther Cancer Institute, 975 West Walnut Street, IB130, Indianapolis, IN 46202, USA
    FEBS Lett 519:93-8. 2002
    ..These studies not only illustrated the counter regulatory function of Smad7 on activin signaling, but also indicated the involvement of phosphorylation at activin type I receptor in the inhibitory action of Smad7...
  31. ncbi request reprint Using magnetic force to enhance immune response to DNA vaccine
    Xian feng Zhou
    College of Life Science, Jilin University, Huoju Road 1260, Changchun 130012, PR China
    Small 3:1707-13. 2007
  32. ncbi request reprint Latent transforming growth factor beta-binding protein 1 interacts with fibrillin and is a microfibril-associated protein
    Zenzo Isogai
    Department of Biochemistry and Molecular Biology, Shriners Hospital for Children, Oregon Health and Science University, Portland 97239, USA
    J Biol Chem 278:2750-7. 2003
    ..In tissues where LTBP-1 is not expressed, LTBP-4 may substitute for LTBP-1, because the C-terminal end of LTBP-4 binds equally well to fibrillin. A model depicting the relationship between LTBP-1 and fibrillin microfibrils is proposed...
  33. ncbi request reprint Application of Multivariate curve resolution-alternating least square methods on the resolution of overlapping CE peaks from different separation conditions
    Fang Zhang
    Institute of Analytical Science, Northwest University, Xi an, People s Republic of China
    Electrophoresis 28:3674-83. 2007
    ....
  34. ncbi request reprint Overexpressed human mitochondrial thioredoxin confers resistance to oxidant-induced apoptosis in human osteosarcoma cells
    Yan Chen
    Program of Biochemistry, Cell, and Developmental Biology, Emory University, Atlanta, Georgia 30322, USA
    J Biol Chem 277:33242-8. 2002
    ..Thus, human mtTrx is a member of the thioredoxin family of proteins localized to mitochondria and may play important roles in protection against oxidant-induced apoptosis...
  35. ncbi request reprint Latent TGF-beta binding protein-3 (LTBP-3) requires binding to TGF-beta for secretion
    Yan Chen
    Department of Cell Biology, New York University School of Medicine, New York, NY 10016, USA
    FEBS Lett 517:277-80. 2002
    ..The secretion of LTBP-3 requires complexing of LTBP-3 with Cys33 of the TGF-beta propeptide...
  36. ncbi request reprint [Effect of Epstein-Barr virus latent membrane protein 1 (LMP1) on apoptosis of nasopharyngeal carcinoma cell line CNE1]
    Yan Chen
    Department of Pathology, Guangdong Medical College, Zhanjiang 524023, P R China
    Ai Zheng 21:498-503. 2002
    ..This study was designed to investigate the effect of EBV-LMP1 on apoptosis of NPC CNE1 cell line and to explore the possible mechanism of the function of EBV-LMP1 in the genesis and development of NPC...
  37. pmc Characterization of a Bcl-XL-interacting protein FKBP8 and its splice variant in human RPE cells
    Yan Chen
    Vanderbilt Eye Institute, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA
    Invest Ophthalmol Vis Sci 49:1721-7. 2008
    ..The purpose of this study was to define the expression of the FKBP8 gene in cultured human RPE cells and explore its involvement in the control of apoptosis...
  38. ncbi request reprint Smad3 mediates activin-induced transcription of follicle-stimulating hormone beta-subunit gene
    Magdalena I Suszko
    Department of Neurobiology and Physiology, Northwestern University, 2205 Tech Drive, Evanston, IL 60208 USA
    Mol Endocrinol 19:1849-58. 2005
    ....
  39. ncbi request reprint Amino acid requirements for formation of the TGF-beta-latent TGF-beta binding protein complexes
    Yan Chen
    Department of Cell Biology, New York University School of Medicine, 550 First Avenue, New York, NY 10016, USA
    J Mol Biol 345:175-86. 2005
    ....
  40. ncbi request reprint Fibronectin is required for integrin alphavbeta6-mediated activation of latent TGF-beta complexes containing LTBP-1
    Laura Fontana
    Department of Cell Biology, New York University School of Medicine, New York, NY 10016, USA
    FASEB J 19:1798-808. 2005
    ..The results indicate specificity for integrin-mediated latent TGF-beta activation that include unique sequences in LTBP-1 and an appropriate matrix molecule...
  41. ncbi request reprint Comparison of diafiltration and tangential flow filtration for purification of nanoparticle suspensions
    Gautam Dalwadi
    Western Australian Biomedical Research Institute, School of Pharmacy, Curtin University of Technology, Perth, Australia
    Pharm Res 22:2152-62. 2005
    ..The study reports evaluation of different purification processes for removing surplus surfactant and formulating stable nanoparticle dispersions...
  42. doi request reprint Using aptamers to study protein-protein interactions
    Parag Parekh
    Department of Chemistry and Department of Physiology and Functional Genomics, Shands Cancer Center, Center for Research at the Bio Nano Interface, University of Florida, Gainesville, FL 32611 7200, USA
    Adv Biochem Eng Biotechnol 110:177-94. 2008
    ..To accomplish this objective, we report the use of DNA/RNA aptamers as a novel tool for the study and elucidation of protein-protein interactions, both in vivo and in vitro...
  43. doi request reprint Characterization of the topology and functional domains of RKTG
    Xiaolin Luo
    Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Graduate School of the Chinese Academy of Sciences, 294 Taiyuan Road, Shanghai 200031, China
    Biochem J 414:399-406. 2008
    ....
  44. doi request reprint RKTG sequesters B-Raf to the Golgi apparatus and inhibits the proliferation and tumorigenicity of human malignant melanoma cells
    Fengjuan Fan
    Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031, China
    Carcinogenesis 29:1157-63. 2008
    ..Collectively, our results suggest that RKTG may play a suppressive role in human melanoma that harbors an oncogenic B-Raf mutation via its antagonistic action on B-Raf...
  45. doi request reprint Endostar suppresses invasion through downregulating the expression of matrix metalloproteinase-2/9 in MDA-MB-435 human breast cancer cells
    Na Lu
    Jiangsu Key Laboratory of Carcinogenesis and Intervention, China Pharmaceutical University, 210009 Nanjing, People s Republic of China
    Exp Biol Med (Maywood) 233:1013-20. 2008
    ..Additionally, endostar exerted an inhibitory effect on the phosphorylation of ERK1/2. Collectively, these data provided a molecular basis for the anti-invasive effects of endostar...
  46. ncbi request reprint The association of calmodulin with central spindle regulates the initiation of cytokinesis in HeLa cells
    Yue Yue Yu
    Jiangsu Key Laboratory for Molecular and Medical Biotechnology, School of Life Sciences, Nanjing Normal University, Nanjing 210097, Jiangsu Province, China
    Int J Biochem Cell Biol 36:1562-72. 2004
    ....
  47. ncbi request reprint [Expression of histone-based fusion protein HNHG in E.coli]
    Fei Han Dai
    State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai 200032, China
    Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 34:800-3. 2002
    ..This specific ability of the fusion protein HNHG of binding and condensing plasmids could be utilized to construct novel exogenous gene delivery systems. HNHG would be a promising candidate for gene delivery...
  48. pmc Large-scale cDNA transfection screening for genes related to cancer development and progression
    Dafang Wan
    National Laboratory for Oncogenes and Related Genes, Cancer Institute of Shanghai Jiao Tong University, 25 Ln 2200 Xie Tu Road, Shanghai 200032, People s Republic of China
    Proc Natl Acad Sci U S A 101:15724-9. 2004
    ..It offers a better understanding of the basic machinery of oncogenesis, including previously undescribed systemic regulatory mechanisms, and also provides a tool for gene discovery with potential clinical applications...
  49. ncbi request reprint Surface amplification of invasive cleavage products
    Yan Chen
    Department of Chemistry, University of Wisconsin Madison, 1101 University Avenue, Madison, Wisconsin 53706 1396, USA
    J Am Chem Soc 126:3016-7. 2004
    ..This combination of very specific surface cleavage and highly sensitive surface detection will make possible the rapid and parallel analysis of genetic variations across large populations...
  50. ncbi request reprint A molecular phylogeny of eurytomid wasps inferred from DNA sequence data of 28S, 18S, 16S, and COI genes
    Yan Chen
    Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China
    Mol Phylogenet Evol 31:300-7. 2004
    ..Subsequent taxonomic revisions include elevating the subfamily Rileyinae to a family status and the divisions of the genera Eurytoma and Bruchophagus...
  51. pmc Suppression of matrix metalloproteinase-9 transcription by transforming growth factor-beta is mediated by a nuclear factor-kappaB site
    Kenji Ogawa
    Department of Medical and Molecular Genetics, Walther Oncology Center, Indiana University School of Medicine and the Walther Cancer Institute, Indianapolis, IN 46202, USA
    Biochem J 381:413-22. 2004
    ..Taken together, these studies indicate that the NF-kappaB site is indispensable for the suppressive activity of TGF-beta in the regulation of MMP-9 transcription...
  52. ncbi request reprint Spatiotemporal pattern of calmodulin and [Ca2+]i is related to resumption of meiosis in mouse oocytes
    Chun Ming Bi
    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China
    Cell Biol Int 28:317-22. 2004
    ..Taking the data from calcium chelation and CaM inhibition together, our results suggest that the dynamic distribution patterns of calcium and activated CaM are crucial for oocyte maturation...
  53. ncbi request reprint [Construction and expression of eukaryotic expression vector containing hVEGF165 gene in rat bone marrow stroma cell]
    Quan wen Gao
    Department of Oral Histology and Pathology, College of Stomatology, Fourth Military Medical University, Xi an 710032, China
    Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 19:32-4. 2003
    ..To construct the eukaryotic expression vector containing human vascular endothelial growth factor 165(VEGF165) gene and express it in rat bone marrow stroma cells(rMSCs)...
  54. ncbi request reprint Sequences of B-chain/domain 1-10/1-9 of insulin and insulin-like growth factor 1 determine their different folding behavior
    Yan Chen
    Key Laboratory of Proteomics, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Graduate School, The Chinese Academy of Sciences, Shanghai 200031, China
    Biochemistry 43:9225-33. 2004
    ....
  55. ncbi request reprint A deterministic iterative least-squares algorithm for beam weight optimization in conformal radiotherapy
    Yan Chen
    Department of Radiation Oncology, Kimmel Cancer Center, Jefferson Medical College of Thomas Jefferson University, Philadelphia, PA 19107, USA
    Phys Med Biol 47:1647-58. 2002
    ..The method is robust in solving difficult clinical cases using either aperture or pencil beam based planning techniques for intensity-modulated radiation therapy...
  56. ncbi request reprint The cyclopentenone product of lipid peroxidation, 15-A(2t)-isoprostane (8-isoprostaglandin A(2)), is efficiently conjugated with glutathione by human and rat glutathione transferase A4-4
    Ina Hubatsch
    Department of Biochemistry, Uppsala University, SE 75123 Uppsala, Sweden
    Chem Res Toxicol 15:1114-8. 2002
    ..These studies have therefore defined a potentially important route by which cyclopentenone IsoPs are metabolized that may serve as a mechanism for the inactivation of these highly reactive compounds...
  57. doi request reprint A highly specific BODIPY-based fluorescent probe for the detection of hypochlorous acid
    Zhen Ning Sun
    Department of Chemistry, The University of Hong Kong, Hong Kong, P R China
    Org Lett 10:2171-4. 2008
    ..This new probe might be used as an efficient tool for probing the roles HOCl plays in biological systems...
  58. ncbi request reprint An optimization algorithm for intensity modulated radiotherapy--the simulated dynamics with dose-volume constraints
    Qing Hou
    Key Lab for Radiation Physics and Technology, Institute of Nuclear Science and Technology, Sichuan University, Chengdu 610064, China
    Med Phys 30:61-8. 2003
    ..In addition to the convenience of implementation, our method can achieve a high computational efficiency with the understanding of the dynamic behavior of the system...
  59. pmc Designing chitosan-dextran sulfate nanoparticles using charge ratios
    Yan Chen
    School of Pharmacy, Curtin University of Technology, GPO Box U1987, Perth, Western Australia
    AAPS PharmSciTech 8:E98. 2007
    ..The CS-DS nanoparticles developed based on the modulation of charge ratio show promise as a system for controlled delivery of both small and large molecules, including proteins...
  60. pmc Atrial natriuretic peptide promotes cardiomyocyte survival by cGMP-dependent nuclear accumulation of zyxin and Akt
    Takahiro Kato
    San Diego State University Heart Institute, Department of Biology, San Diego, California 92182, USA
    J Clin Invest 115:2716-30. 2005
    ..Nuclear accumulation of zyxin and activated Akt may represent a fundamental mechanism that facilitates nuclear-signal transduction and potentiates cell survival...
  61. ncbi request reprint [Transduction efficiency of recombinant adeno-associated virus 2 in human umbilical cord blood CD34+ hematopoietic stem/progenitor cells]
    Yan Chen
    Department of Hematology, Xiangya Hospital of Central South University, Changsha 410008, China
    Zhongguo Shi Yan Xue Ye Xue Za Zhi 11:576-8. 2003
    ..The results showed that 43% CD34(+) cells expressed the GFP gene at a multiplicity of infection of 2 x 10(5). It is concluded that the rAAV-2 can transduce human cord blood CD34(+) hematopoietic stem/progenitor cells efficiently...
  62. ncbi request reprint Tumor-derived C-terminal mutations of Smad4 with decreased DNA binding activity and enhanced intramolecular interaction
    Chenzhong Kuang
    Department of Medical and Molecular Genetics, Indiana University School of Medicine and the Walther Cancer Institute, Indianapolis, IN 46202, USA
    Oncogene 23:1021-9. 2004
    ..Therefore, these studies revealed novel mechanisms by which the Smad4 mutations utilize to abrogate their functions in transducing the signaling of TGF-beta, which plays an important role in various stages of cancer formation...
  63. ncbi request reprint IDGraphs: intrusion detection and analysis using stream compositing
    Pin Ren
    Department of Electrical Engineering and Computer Science, Northwestern University, USA
    IEEE Comput Graph Appl 26:28-39. 2006
  64. ncbi request reprint Antagonists to human and mouse vascular endothelial growth factor receptor 2 generated by directed protein evolution in vitro
    Elena V Getmanova
    Phylos, Inc, succeeded by Compound Therapeutics, Inc, 100 Beaver Street, Waltham, Massachusetts 02453, USA
    Chem Biol 13:549-56. 2006
    ..Proteins encoded by the selected clones bound VEGFR2-expressing cells and inhibited their VEGF-dependent proliferation. Our results demonstrate the potential of these inhibitors in the development of anti-angiogenesis therapeutics...
  65. ncbi request reprint Functional silencing of hepatic microsomal glucose-6-phosphatase gene expression in vivo by adenovirus-mediated delivery of short hairpin RNA
    Alan Huang
    Millennium Pharmaceuticals, 75 Sidney St, Cambridge, MA 02139, USA
    FEBS Lett 558:69-73. 2004
    ..These findings provide evidence that AdV delivery of expressed shRNA can be a productive tool to explore gene function in vivo...
  66. ncbi request reprint Augmenter of liver regeneration promotes hepatocyte proliferation induced by Kupffer cells
    Chun Ping Wang
    Department of Gastroenterology, 302 Hospital of Chinese PLA, 100 Xisihuan Middle Road, Beijing 100039, China
    World J Gastroenterol 12:4859-65. 2006
    ..To observe the effects of augmenter of liver regeneration (ALR) on Kupffer cells and to determine whether ALR promotes hepatocyte proliferation induced by Kupffer cells...
  67. ncbi request reprint Activated c-Fms recruits Vav and Rac during CSF-1-induced cytoskeletal remodeling and spreading in osteoclasts
    Hiroaki Sakai
    Yale School of Medicine, TAC S 133, PO Box 208020, New Haven, CT 06520 8020, USA
    Bone 39:1290-301. 2006
    ..These data demonstrate that Vav and Rac lie downstream from activated PI3-K in CSF-1-treated osteoclasts and that Rac is required for CSF-1-induced cytoskeletal remodeling in these cells...
  68. ncbi request reprint [Interleukin-12 enhanced tumor necrosis factor related apoptosis-inducing ligand TRAIL-induced apoptosis in human hepatocellular carcinoma by inhibiting expression of survivin]
    Song Qing He
    Hepatic Surgery Center, Affiliated Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
    Zhonghua Wai Ke Za Zhi 41:453-7. 2003
    ..To investigate therapeutic potential of TRAIL in hepatocellular carcinoma (HCC) and the mechanism of sTRAIL resistance and to reverse the resistance to sTRAIL-inducing apoptosis...
  69. ncbi request reprint Transcriptional regulation of tristetraprolin by transforming growth factor-beta in human T cells
    Kenji Ogawa
    Department of Medical and Molecular Genetics, Walther Oncology Center, Indiana University School of Medicine and the Walther Cancer Institute, Indianapolis, Indiana 46202, USA
    J Biol Chem 278:30373-81. 2003
    ....
  70. ncbi request reprint Enhance immune response to DNA vaccine based on a novel multicomponent supramolecular assembly
    Xianfeng Zhou
    College of Life Science, Key Laboratory for Supramolecular Structure and Materials of Ministry of Education, Jilin University, Changchun 130012, and Sichuan Tumor Hospital and Institute, Chengdu, PR China
    Biomaterials 28:4684-92. 2007
    ..Consequently, formulation of DNA vaccines into multicomponent vectors is a powerful means of increasing vaccine potency...
  71. ncbi request reprint Controlled release of PEI/DNA complexes from PLGA microspheres as a potent delivery system to enhance immune response to HIV vaccine DNA prime/MVA boost regime
    Xianfeng Zhou
    College of Life Science, Jilin University, Changchun, PR China
    Eur J Pharm Biopharm 68:589-95. 2008
    ..m. immunization, the microspheres induced significantly enhanced serum antibody responses 2-3 orders of magnitude greater than naked DNA. Additionally, in contrast to naked DNA, the microspheres induced potent CTL responses at a low dose...
  72. ncbi request reprint The key virulence-associated genes of Streptococcus suis type 2 are upregulated and differentially expressed in vivo
    Chen Tan
    College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
    FEMS Microbiol Lett 278:108-14. 2008
    ..Characterization of the differential expression patterns of this subset of key virulence-associated genes facilitates further study into the importance of these genes in S. suis serotype 2 pathogenesis in pigs...
  73. pmc Characterization of HPV16 L1 loop domains in the formation of a type-specific, conformational epitope
    Vanessa A Olcese
    Department of Pathology, Georgetown University Medical Center, Washington, DC 20057, USA
    BMC Microbiol 4:29. 2004
    ....
  74. ncbi request reprint Beam orientation optimization for IMRT by a hybrid method of the genetic algorithm and the simulated dynamics
    Qing Hou
    Key Lab for Radiation Physics and Technology, Institute of Nuclear Science and Technology, Sichuan University, Chengdu 610064, China
    Med Phys 30:2360-7. 2003
    ..g., the smoothness of intensity profiles, for the selection of the optimal plan among the degenerate configurations for treatment delivery...
  75. doi request reprint Light-switching excimer beacon assays for ribonuclease H kinetic study
    Yan Chen
    Center for Research at the Bio Nano Interface, Department of Chemistry, UF Genetics Institute, Shands Cancer Center and McKnight Brain Institute, University of Florida, Gainesville, FL 32611 7200, USA
    Chembiochem 9:355-9. 2008
  76. ncbi request reprint [Apoptosis of Fas(+) Yac-1 cells induced with Fas ligand-transfected COS-7 cells]
    Ling Bo Liu
    Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
    Zhongguo Shi Yan Xue Ye Xue Za Zhi 10:327-31. 2002
    ..01), in which the COS-7 cell was transfected by pBillneo (not carrying mFasL gene). It was concluded that lymphoma cells highly expressing Fas can be effectively killed through single Fas-FasL way in vitro...

Research Grants1

  1. In Vivo fluorescence correlation spectroscopy
    Yan Chen; Fiscal Year: 2003
    ..In addition, GFP tagged retinoid receptors will be used to perform an in vivo study to resolve their molecular oligomerization state in the presence and absence of ligands. ..