Xuequn Chen

Summary

Affiliation: University of Michigan
Country: USA

Publications

  1. ncbi request reprint Dominant negative Rab3D inhibits amylase release from mouse pancreatic acini
    Xuequn Chen
    Department of Physiology, The University of Michigan, Ann Arbor, Michigan 48109 0622, USA
    J Biol Chem 277:18002-9. 2002
  2. ncbi request reprint Dominant negative Rab3D mutants reduce GTP-bound endogenous Rab3D in pancreatic acini
    Xuequn Chen
    Department of Molecular and Integrative Physiology, The University of Michigan, Ann Arbor, Michigan 48109, USA
    J Biol Chem 278:50053-60. 2003
  3. ncbi request reprint Rab27b localizes to zymogen granules and regulates pancreatic acinar exocytosis
    Xuequn Chen
    Department of Molecular and Integrative Physiology, The University of Michigan, Ann Arbor, MI, USA
    Biochem Biophys Res Commun 323:1157-62. 2004
  4. ncbi request reprint Organellar proteomics: analysis of pancreatic zymogen granule membranes
    Xuequn Chen
    Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48109, USA
    Mol Cell Proteomics 5:306-12. 2006
  5. pmc Global topology analysis of pancreatic zymogen granule membrane proteins
    Xuequn Chen
    Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA
    Mol Cell Proteomics 7:2323-36. 2008
  6. pmc Quantitative organellar proteomics analysis of rough endoplasmic reticulum from normal and acute pancreatitis rat pancreas
    Xuequn Chen
    Departments of Molecular and Integrative Physiology, The University of Michigan, Ann Arbor, Michigan 48109, USA
    J Proteome Res 9:885-96. 2010
  7. pmc Quantitative analysis of liver Golgi proteome in the cell cycle
    Xuequn Chen
    Department of Physiology, Wayne State University, Detroit, MI, USA
    Methods Mol Biol 909:125-40. 2012
  8. pmc Quantitative proteomics analysis of cell cycle-regulated Golgi disassembly and reassembly
    Xuequn Chen
    Department of Molecular and Integrative Physiology, The University of Michigan, Ann Arbor, Michigan 48109 1048, USA
    J Biol Chem 285:7197-207. 2010
  9. pmc Molecular characterization of the endoplasmic reticulum: insights from proteomic studies
    Xuequn Chen
    Department of Molecular and Integrative Physiology, The University of Michigan, Ann Arbor, MI, USA
    Proteomics 10:4040-52. 2010
  10. doi request reprint Quantitative proteomics analysis of pancreatic zymogen granule membrane proteins
    Xuequn Chen
    Department of Biological Chemistry, University of Michigan, Ann Arbor, MI, USA
    Methods Mol Biol 528:327-38. 2009

Collaborators

Detail Information

Publications18

  1. ncbi request reprint Dominant negative Rab3D inhibits amylase release from mouse pancreatic acini
    Xuequn Chen
    Department of Physiology, The University of Michigan, Ann Arbor, Michigan 48109 0622, USA
    J Biol Chem 277:18002-9. 2002
    ..These results suggest that Rab3D plays an important role in regulating the terminal steps of acinar exocytosis and that this effect is greatest on the early phase of amylase release...
  2. ncbi request reprint Dominant negative Rab3D mutants reduce GTP-bound endogenous Rab3D in pancreatic acini
    Xuequn Chen
    Department of Molecular and Integrative Physiology, The University of Michigan, Ann Arbor, Michigan 48109, USA
    J Biol Chem 278:50053-60. 2003
    ..6 +/- 3.3%. The two dominant negative Rab3D mutants, therefore, interfere with endogenous Rab3D function by blocking the GDP/GTP exchange but not zymogen granule targeting of endogenous Rab3D...
  3. ncbi request reprint Rab27b localizes to zymogen granules and regulates pancreatic acinar exocytosis
    Xuequn Chen
    Department of Molecular and Integrative Physiology, The University of Michigan, Ann Arbor, MI, USA
    Biochem Biophys Res Commun 323:1157-62. 2004
    ..This enhancement and inhibition occurred at all points on the CCK dose-response curve and over a 30min time course. These results demonstrate that Rab27b is present on ZGs and plays an important role in regulating acinar exocytosis...
  4. ncbi request reprint Organellar proteomics: analysis of pancreatic zymogen granule membranes
    Xuequn Chen
    Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48109, USA
    Mol Cell Proteomics 5:306-12. 2006
    ....
  5. pmc Global topology analysis of pancreatic zymogen granule membrane proteins
    Xuequn Chen
    Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA
    Mol Cell Proteomics 7:2323-36. 2008
    ..This model contributes to a firm foundation for developing a higher order architecture model of the ZGM and for future functional studies of individual ZGM proteins...
  6. pmc Quantitative organellar proteomics analysis of rough endoplasmic reticulum from normal and acute pancreatitis rat pancreas
    Xuequn Chen
    Departments of Molecular and Integrative Physiology, The University of Michigan, Ann Arbor, Michigan 48109, USA
    J Proteome Res 9:885-96. 2010
    ..These results suggest that the early stages of AP involve changes of multiple RER proteins that may affect the synthesis and processing of digestive enzymes...
  7. pmc Quantitative analysis of liver Golgi proteome in the cell cycle
    Xuequn Chen
    Department of Physiology, Wayne State University, Detroit, MI, USA
    Methods Mol Biol 909:125-40. 2012
    ..Altogether, this systematic quantitative proteomic study revealed candidate proteins of the molecular machinery that controls the Golgi disassembly and reassembly processes in the cell cycle...
  8. pmc Quantitative proteomics analysis of cell cycle-regulated Golgi disassembly and reassembly
    Xuequn Chen
    Department of Molecular and Integrative Physiology, The University of Michigan, Ann Arbor, Michigan 48109 1048, USA
    J Biol Chem 285:7197-207. 2010
    ..Altogether, this systematic quantitative proteomic study revealed candidate proteins of the molecular machinery that control the Golgi disassembly and reassembly processes in the cell cycle...
  9. pmc Molecular characterization of the endoplasmic reticulum: insights from proteomic studies
    Xuequn Chen
    Department of Molecular and Integrative Physiology, The University of Michigan, Ann Arbor, MI, USA
    Proteomics 10:4040-52. 2010
    ....
  10. doi request reprint Quantitative proteomics analysis of pancreatic zymogen granule membrane proteins
    Xuequn Chen
    Department of Biological Chemistry, University of Michigan, Ann Arbor, MI, USA
    Methods Mol Biol 528:327-38. 2009
    ..The proteins with 117:114 ratios greater than one correlated well with known or predicted intrinsic membrane proteins...
  11. pmc EPI64B acts as a GTPase-activating protein for Rab27B in pancreatic acinar cells
    Yanan Hou
    Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, Michigan 48109, USA
    J Biol Chem 288:19548-57. 2013
    ..These data suggest that EPI64B can serve as a potential physiological GAP for Rab27B and thereby participate in the regulation of exocytosis in pancreatic acinar cells. ..
  12. pmc Rap1 activation plays a regulatory role in pancreatic amylase secretion
    Maria E Sabbatini
    Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, MI 48109 0622, USA
    J Biol Chem 283:23884-94. 2008
    ..Rap1 activation not only mediates the cAMP-evoked response via Epac1 but is also involved in CCK- and carbachol-induced amylase release, with their action most likely mediated by CalDAG-GEF III...
  13. ncbi request reprint Regulation of CCK-induced amylase release by PKC-delta in rat pancreatic acinar cells
    Chenwei Li
    Dept of Molecular and Integrative Physiology, Univ of Michigan, 7744 Medical Science II, Ann Arbor, MI 48109 0622, USA
    Am J Physiol Gastrointest Liver Physiol 287:G764-71. 2004
    ..GF109203X totally blocked the enhancement of amylase release by PKC-delta but had no further effect in the presence of dominant-negative PKC-delta. These results indicate that PKC-delta is the PKC isoform involved with amylase secretion...
  14. doi request reprint Aquaporin-assisted and ER-mediated mitochondrial fission: a hypothesis
    Jin Sook Lee
    Department of Physiology, School of Medicine, Wayne State University, Detroit, MI 48201, USA
    Micron 47:50-8. 2013
    ....
  15. doi request reprint Purification and proteomics analysis of pancreatic zymogen granule membranes
    Xuequn Chen
    Department of Biological Chemistry, The University of Michigan, Ann Arbor, MI, USA
    Methods Mol Biol 432:275-87. 2008
    ..The proteins with 117:114 ratios greater than one correlated well with intrinsic membrane proteins that contain either known or predicted transmembrane domains...
  16. pmc Small G proteins as key regulators of pancreatic digestive enzyme secretion
    John A Williams
    Dept of Molecular and Integrative Physiology, Univ of Michigan, Ann Arbor, MI 48109, USA
    Am J Physiol Endocrinol Metab 296:E405-14. 2009
    ..The Rho family small G proteins RhoA and Rac1 also regulate secretion through remodeling of the actin cytoskeleton. Possible mechanisms for regulation of these G proteins and their effector molecules are considered...
  17. doi request reprint Neuronal porosome proteome: Molecular dynamics and architecture
    Jin Sook Lee
    Department of Physiology, Wayne State University School of Medicine, Detroit, MI 48201, USA
    J Proteomics 75:3952-62. 2012
    ..The association and dissociation of proteins at the porosome following stimulation of cell secretion demonstrate the dynamic nature of the organelle...
  18. doi request reprint Direct interaction between Rab3D and the polymeric immunoglobulin receptor and trafficking through regulated secretory vesicles in lacrimal gland acinar cells
    Eunbyul Evans
    Dept of Pharmacology and Pharmaceutical Sciences, Univ of Southern California, Los Angeles, CA 90089 9121, USA
    Am J Physiol Cell Physiol 294:C662-74. 2008
    ..These data suggest that the novel localization of pIgR to the regulated secretory pathway of LGACs and its secretion therefrom may be affected by its novel interaction with Rab3D...