IRVIN SY CHEN

..This is problematic for comparing hot-spot distributions across data sets and patients, where the VIS numbers may vary...

..These results have implications for the application of RNAi technology for therapeutic purposes...

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..We propose to model siRNA to HIV-1 and CCR5 using lentiviral vectors for stable delivery. ..

..abstract_text> ..

..PUBLIC HEALTH RELEVANCE: Relevance RNA interference is a means to block the function of genes. We will investigate whether RNA interference can be used as a potential therapy for HIV-1 disease. ..

..This understanding will, in turn, provide us with critical information needed to understand the role of Vpr/HHR23A interaction and to develop potential AIDS therapeutic agents based upon the interaction between these proteins. ..

..3) Based upon the results of Aims 1 and 2, test the effectiveness of the best anti-HIV gene therapeutic vectors to inhibit HIV-1 1/SIV replication in in vivo model systems of SCID-hu mice and rhesus macaque. ..

..Given our long-standing interest in retroviral biology, recently applied to gene therapy vectors, and the novel targeting approach described here, we feel that we are well positioned to address the questions posed in this application. ..

..Given our long-standing interest in retroviral biology, recent development of gene therapy vectors and studies in mice and non-human primates, we feel that we are well positioned to address the questions posed in this application. ..

..The proposed studies address fundamental questions regarding the mode of action of the targeting vector and its utility for molecular imaging. ..

..At UCLA, this rigorous training program with emphasis on vertical integration of basic sciences and therapeutic applications will produce scientists required for long-term development of gene therapy. ..

..The proposed studies address fundamental questions regarding the mode of action of the targeting vector and its utility for molecular imaging. ..

..Aim 2) To characterize the newly described process of Vpr mediated transactivation of unintegrated HIV-1 DNA. Aim 3) To further characterize the role of Vpr in viral pathogenesis. ..

..Ideally, structures of both the free Vpr and the complex will be determined. However, since Vpr is known to oligomerize, it may only be possible to determine its structure in the complex. ..

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..The specific aims are: Aim 1. To further characterize cell cycle arrest by HIV-1 Vpr. Aim 2. To further characterize Vpr mediated apoptosis Aim 3. To identify cellular factors involved in Vpr-mediated cell cycle arrest and apoptosis. ..