Wenhan Chang

Summary

Affiliation: University of California
Country: USA

Publications

  1. pmc The extracellular calcium-sensing receptor (CaSR) is a critical modulator of skeletal development
    Wenhan Chang
    Endocrine Research Unit, Department of Veterans Affairs Medical Center, Department of Medicine, University of California, San Francisco, CA 94121, USA
    Sci Signal 1:ra1. 2008
  2. ncbi request reprint Complex formation with the Type B gamma-aminobutyric acid receptor affects the expression and signal transduction of the extracellular calcium-sensing receptor. Studies with HEK-293 cells and neurons
    Wenhan Chang
    Endocrine Research Unit, Department of Veterans Affairs Medical Center, Department of Medicine, University of California, San Francisco, California 94121, USA
    J Biol Chem 282:25030-40. 2007
  3. ncbi request reprint Type B gamma-aminobutyric acid receptors modulate the function of the extracellular Ca2+-sensing receptor and cell differentiation in murine growth plate chondrocytes
    Zhiqiang Cheng
    Endocrine Research Unit, Department of Veterans Affairs Medical Center, 4150 Clement Street, San Francisco, CA 94121, USA
    Endocrinology 148:4984-92. 2007
  4. pmc IGF-1R signaling in chondrocytes modulates growth plate development by interacting with the PTHrP/Ihh pathway
    Yongmei Wang
    Endocrine Unit, University of California, Veterans Affairs Medical Center, San Francisco, CA, USA
    J Bone Miner Res 26:1437-46. 2011
  5. ncbi request reprint Expression and functional assessment of an alternatively spliced extracellular Ca2+-sensing receptor in growth plate chondrocytes
    Luis Rodriguez
    Endocrine Research Unit, Department of Medicine, Department of Veterans Affairs Medical Center, University of California, San Francisco, 94121, USA
    Endocrinology 146:5294-303. 2005
  6. pmc Osteoblast extracellular Ca2+ -sensing receptor regulates bone development, mineralization, and turnover
    Melita M Dvorak-Ewell
    Endocrine Research Unit, Department of Veterans Affairs Medical Center, Department of Medicine, University of California, San Francisco, CA 94121, USA
    J Bone Miner Res 26:2935-47. 2011
  7. ncbi request reprint Inactivation of the calcium sensing receptor inhibits E-cadherin-mediated cell-cell adhesion and calcium-induced differentiation in human epidermal keratinocytes
    Chia Ling Tu
    Endocrine Unit, Veteran Affairs Medical Center, University of California, San Francisco, California 94121, USA
    J Biol Chem 283:3519-28. 2008
  8. ncbi request reprint Constitutive activity of the osteoblast Ca2+-sensing receptor promotes loss of cancellous bone
    Melita M Dvorak
    Endocrine Research Unit, Department of Veterans Affairs Medical Center, 4150 Clement Street, University of California, San Francisco, California 94121, USA
    Endocrinology 148:3156-63. 2007
  9. pmc Ablation of the calcium-sensing receptor in keratinocytes impairs epidermal differentiation and barrier function
    Chia Ling Tu
    Endocrine Unit, Veterans Affairs Medical Center, University of California, San Francisco, San Francisco, California 94121, USA
    J Invest Dermatol 132:2350-9. 2012
  10. ncbi request reprint Insulin-like growth factor-I is essential for embryonic bone development
    Yongmei Wang
    Department of Medicine, Endocrine Unit, 111N, Veterans Affairs Medical Center, 4150 Clement Street, San Francisco, California 94121, USA
    Endocrinology 147:4753-61. 2006

Collaborators

Detail Information

Publications23

  1. pmc The extracellular calcium-sensing receptor (CaSR) is a critical modulator of skeletal development
    Wenhan Chang
    Endocrine Research Unit, Department of Veterans Affairs Medical Center, Department of Medicine, University of California, San Francisco, CA 94121, USA
    Sci Signal 1:ra1. 2008
    ..Mice in which chondrocyte-specific deletion of Casr was induced between E16 and E18 were viable but showed delayed growth plate development. Our data show a critical role for the CaSR in early embryogenesis and skeletal development...
  2. ncbi request reprint Complex formation with the Type B gamma-aminobutyric acid receptor affects the expression and signal transduction of the extracellular calcium-sensing receptor. Studies with HEK-293 cells and neurons
    Wenhan Chang
    Endocrine Research Unit, Department of Veterans Affairs Medical Center, Department of Medicine, University of California, San Francisco, California 94121, USA
    J Biol Chem 282:25030-40. 2007
    ....
  3. ncbi request reprint Type B gamma-aminobutyric acid receptors modulate the function of the extracellular Ca2+-sensing receptor and cell differentiation in murine growth plate chondrocytes
    Zhiqiang Cheng
    Endocrine Research Unit, Department of Veterans Affairs Medical Center, 4150 Clement Street, San Francisco, CA 94121, USA
    Endocrinology 148:4984-92. 2007
    ..These results support the idea that physical interactions between CaRs and GABA-B-R1s modulate the growth and differentiation of GPCs, potentially by altering the function of CaRs...
  4. pmc IGF-1R signaling in chondrocytes modulates growth plate development by interacting with the PTHrP/Ihh pathway
    Yongmei Wang
    Endocrine Unit, University of California, Veterans Affairs Medical Center, San Francisco, CA, USA
    J Bone Miner Res 26:1437-46. 2011
    ..Our data indicate that the IGF-1R in chondrocytes controls cell growth, survival, and differentiation in embryonic and postnatal GPs in part by suppression of Pthrp expression...
  5. ncbi request reprint Expression and functional assessment of an alternatively spliced extracellular Ca2+-sensing receptor in growth plate chondrocytes
    Luis Rodriguez
    Endocrine Research Unit, Department of Medicine, Department of Veterans Affairs Medical Center, University of California, San Francisco, 94121, USA
    Endocrinology 146:5294-303. 2005
    ..Taken together, our data support the idea that expression of (Exon5(-))CaRs may compensate for the loss of full-length CaRs and be responsible for sensing changes in [Ca(2+)](e) in GPCs in CaR(-/-) mice...
  6. pmc Osteoblast extracellular Ca2+ -sensing receptor regulates bone development, mineralization, and turnover
    Melita M Dvorak-Ewell
    Endocrine Research Unit, Department of Veterans Affairs Medical Center, Department of Medicine, University of California, San Francisco, CA 94121, USA
    J Bone Miner Res 26:2935-47. 2011
    ....
  7. ncbi request reprint Inactivation of the calcium sensing receptor inhibits E-cadherin-mediated cell-cell adhesion and calcium-induced differentiation in human epidermal keratinocytes
    Chia Ling Tu
    Endocrine Unit, Veteran Affairs Medical Center, University of California, San Francisco, California 94121, USA
    J Biol Chem 283:3519-28. 2008
    ..Our results indicate that the CaR regulates cell survival and Ca(2+)(o)-induced differentiation in keratinocytes at least in part by activating the E-cadherin/PI3K pathway through a Src family tyrosine kinase-mediated signaling...
  8. ncbi request reprint Constitutive activity of the osteoblast Ca2+-sensing receptor promotes loss of cancellous bone
    Melita M Dvorak
    Endocrine Research Unit, Department of Veterans Affairs Medical Center, 4150 Clement Street, University of California, San Francisco, California 94121, USA
    Endocrinology 148:3156-63. 2007
    ....
  9. pmc Ablation of the calcium-sensing receptor in keratinocytes impairs epidermal differentiation and barrier function
    Chia Ling Tu
    Endocrine Unit, Veterans Affairs Medical Center, University of California, San Francisco, San Francisco, California 94121, USA
    J Invest Dermatol 132:2350-9. 2012
    ..3%) diet. Deleting CaR perturbs the epidermal Ca(2+) gradient and impairs keratinocyte differentiation and permeability barrier homeostasis, indicating a key role for the CaR in normal epidermal development...
  10. ncbi request reprint Insulin-like growth factor-I is essential for embryonic bone development
    Yongmei Wang
    Department of Medicine, Endocrine Unit, 111N, Veterans Affairs Medical Center, 4150 Clement Street, San Francisco, California 94121, USA
    Endocrinology 147:4753-61. 2006
    ..Our results indicate that IGF-I plays an important role in skeletal development by promoting chondrocyte proliferation and maturation while inhibiting apoptosis to form bones of appropriate size and strength...
  11. pmc The calcium-sensing receptor-dependent regulation of cell-cell adhesion and keratinocyte differentiation requires Rho and filamin A
    Chia Ling Tu
    Endocrine Unit, Veteran Affairs Medical Center and University of California, San Francisco, California 94121, USA
    J Invest Dermatol 131:1119-28. 2011
    ..These results indicate that Rho is a downstream mediator of CaR in the regulation of Ca(2+)(o)-induced E-cadherin-mediated cell-cell adhesion and keratinocyte differentiation...
  12. pmc Sex and age modify biochemical and skeletal manifestations of chronic hyperparathyroidism by altering target organ responses to Ca2+ and parathyroid hormone in mice
    Zhiqiang Cheng
    Endocrine Research Unit, Department of Veterans Affairs Medical Center, Department of Medicine, University of California, San Francisco, CA, USA
    J Bone Miner Res 28:1087-100. 2013
    ..Restraining the ability of the PTG to upregulate CaSRs by heterozygous gene deletion contributes to biochemical and skeletal manifestations of HPT, especially in aging females...
  13. ncbi request reprint The role of the calcium sensing receptor in regulating intracellular calcium handling in human epidermal keratinocytes
    Chia Ling Tu
    Endocrine Unit, Department of Medicine, Veteran Affairs Medical Center and University of California, San Francisco, California 94121, USA
    J Invest Dermatol 127:1074-83. 2007
    ..Our results indicate that CaR regulates keratinocyte differentiation in part by modulating Ca2+(i) stores via interactions with Ca2+ pumps and channels that regulate those stores...
  14. ncbi request reprint Extracellular calcium and parathyroid hormone-related peptide signaling modulate the pace of growth plate chondrocyte differentiation
    Luis Rodriguez
    Endocrine Research Unit, Department of Veterans Affairs Medical Center, University of California, San Francisco, California 94121, USA
    Endocrinology 146:4597-608. 2005
    ....
  15. pmc Negative cross-talk between calcium-sensing receptor and β-catenin signaling systems in colonic epithelium
    Osvaldo Rey
    Unit of Signal Transduction and Gastrointestinal Cancer, Division of Digestive Diseases, Department of Medicine, CURE Digestive Diseases Research Center, David Geffen School of Medicine, UCLA, Los Angeles, California 90095, USA
    J Biol Chem 287:1158-67. 2012
    ..These studies demonstrate that signaling pathways emanating from the CaSR control colonic epithelial cell proliferation in vivo and suggest that the mechanism involves regulation of β-catenin phosphorylation...
  16. pmc Naturally-occurring mutation in the calcium-sensing receptor reveals the significance of extracellular domain loop III region for class C G-protein-coupled receptor function
    Qing Dong
    Department of Pediatrics, University of California, San Francisco, San Francisco, California 94143 0434, USA
    J Clin Endocrinol Metab 95:E245-52. 2010
    ..Most mutations are clustered in the N-terminal and Cys-rich regions of the extracellular domain (ECD) and seven-transmembrane domain. Disease-causing mutations are uncommon in the C terminus of ECD...
  17. ncbi request reprint Extracellular Ca(2+)-sensing receptors modulate matrix production and mineralization in chondrogenic RCJ3.1C5.18 cells
    Wenhan Chang
    Endocrine Research Unit, Department of Veterans Affairs Medical Center, University of California, San Francisco, California 94121, USA
    Endocrinology 143:1467-74. 2002
    ..These data support a role for CaRs in mediating high [Ca(2+)](o)-induced differentiation of C5.18 cells...
  18. pmc Assessing constitutive activity of extracellular calcium-sensing receptors in vitro and in bone
    Wenhan Chang
    Endocrine Research Unit, Department of Veterans Affairs Medical Center, Department of Medicine, University of California, San Francisco, California, USA
    Methods Enzymol 484:253-66. 2010
    ..These studies indicate that this approach can be readily adapted to assess CaSR actions in other cell systems...
  19. ncbi request reprint Extracellular Ca2+-sensing receptors--an overview
    Wenhan Chang
    Endocrine Research Unit, Department of Medicine, San Francisco Department of Veterans Affairs Medical Center, University of California, San Francisco, CA, USA
    Cell Calcium 35:183-96. 2004
    ..Studies of CaR-knockout mice confirm the importance of CaRs in the parathyroid gland and kidney. The functions of CaRs in tissues other than kidney and parathyroid gland, however, remain to be elucidated...
  20. ncbi request reprint Ephrin B2/EphB4 Mediates the Actions of IGF-I Signaling in Regulating Endochondral Bone Formation
    Yongmei Wang
    Endocrine Unit, University of California, Veterans Affairs Medical Center, San Francisco, CA, USA
    J Bone Miner Res 29:1900-13. 2014
    ..Our data indicate that IGF-I/IGF-IR signaling promotes OB, OCL, and chondrocyte differentiation via ephrin B2/EphB4 mediated cell-cell communication. © 2014 American Society for Bone and Mineral Research. ..
  21. pmc Hypothermia and pharmacological regimens that prevent overexpression and overactivity of the extracellular calcium-sensing receptor protect neurons against traumatic brain injury
    Jong Youl Kim
    University of California, San Francisco, California, USA
    J Neurotrauma 30:1170-6. 2013
    ....
  22. ncbi request reprint Phospholipase cgamma1 is required for activation of store-operated channels in human keratinocytes
    Chia Ling Tu
    Endocrine Unit, Veteran Affairs Medical Center and Department of Medicine, University of California, San Francisco, California 94121, USA
    J Invest Dermatol 124:187-97. 2005
    ..Our results indicate that the activation of SOC in keratinocytes depends, at least partly, on the interaction of TRPC with PLCgamma1 and IP3R...
  23. ncbi request reprint cDNA cloning and functional expression of a Ca2+-sensing receptor with truncated C-terminal tail from the Mozambique tilapia (Oreochromis mossambicus)
    Christopher A Loretz
    National Science Foundation Tokyo Regional Office, American Embassy, 1 10 5, Akasaka, Minato ku, Tokyo 107 8420, Japan
    J Biol Chem 279:53288-97. 2004
    ..Sensitivity of phospholipase C activation to [Ca(2+)](o) was dependent on the ionic strength of the bathing medium, supporting a role in salinity sensing...

Research Grants10

  1. Calcium-Sensing Receptors and Growth Plate Development
    Wenhan Chang; Fiscal Year: 2002
    ....
  2. Calcium-Sensing Receptors and Growth Plate Development
    Wenhan Chang; Fiscal Year: 2009
    ..This proposal will investigate how Ca2+ acts on these chondrocytes and will help us understand how to treat disorders of growth and poor mineralization like rickets. ..
  3. INTERACTIONS BETWEEN GABA B AND CA2+-SENSING RECEPTORS
    Wenhan Chang; Fiscal Year: 2007
    ..This work can open up future studies targeted to developing therapies to modulate the function of CaRs, GABA-B-Rs, or the heteromeric complexes of these two receptors. ..
  4. Calcium-Sensing Receptors and Growth Plate Development
    Wenhan Chang; Fiscal Year: 2005
    ....
  5. Resolution of Stage-Specific Growth Plate Chondrocytes
    Wenhan Chang; Fiscal Year: 2004
    ..The effects of PTHrP on cyclic AMP (cAMP) formation, phospholipase C (PLC) activity, and intracellular Ca 2. mobilization will be examined in different subpopulations of chondrocytes. ..
  6. Calcium-Sensing Receptors and Growth Plate Development
    Wenhan Chang; Fiscal Year: 2004
    ....
  7. Calcium-Sensing Receptors and Growth Plate Development
    Wenhan Chang; Fiscal Year: 2003
    ....
  8. Calcium-Sensing Receptors and Growth Plate Development
    Wenhan Chang; Fiscal Year: 2010
    ..This proposal will investigate how Ca2+ acts on these chondrocytes and will help us understand how to treat disorders of growth and poor mineralization like rickets. ..