Research Topics
Genomes and Genes | Guillaume F ChanfreauSummaryAffiliation: University of California Country: USA Publications
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Publications
Recognition of a conserved class of RNA tetraloops by Saccharomyces cerevisiae RNase IIIG Chanfreau
Unité de Génétique des Interactions Macromoléculaires, URA1300 Centre National de la Recherche Scientifique, Departement des Biotechnologies, Institut Pasteur, Paris, France
Proc Natl Acad Sci U S A 97:3142-7. 2000..These results also indicate that proteins belonging to the same class of RNA endonucleases require different structural elements for RNA cleavage...
InvIGOrating mRNAs during QuiescenceGuillaume Chanfreau
Department of Chemistry and Biochemistry and the Molecular Biology Institute, University of California, Los Angeles, Box 951569, Los Angeles, CA 90095 1569, USA
Mol Cell 38:317-8. 2010..In this issue of Molecular Cell, De Virgilio and colleagues (Talarek et al., 2010) identify the Igo1/2 proteins as factors that protect mRNAs from degradation during quiescence...- Conservation of RNase III processing pathways and specificity in hemiascomycetesGuillaume Chanfreau
Department of Chemistry and Biochemistry and the Molecular Biology Institute, University of California Los Angeles, Los Angeles, California 90095 1569, USA
Eukaryot Cell 2:901-9. 2003..This analysis shows that RNase III processing signals and RNase III cleavage specificity are conserved in most Hemiascomycetes species but probably not in the evolutionarily more distant species Y. lipolytica... - CUTting genetic noise by polyadenylation-induced RNA degradationGuillaume F Chanfreau
Department of Chemistry and Biochemistry and the Molecular Biology Institute, University of California Los Angeles, Los Angeles, CA 90095 1569, USA
Trends Cell Biol 15:635-7. 2005..These cryptic transcripts are polyadenylated at their 3'-end by a poly(A) polymerase complex distinct from that used by the mRNA factory, which serves to tag these aberrant transcripts for nuclear degradation... - Quality control of MATa1 splicing and exon skipping by nuclear RNA degradationDefne E Egecioglu
Department of Chemistry and Biochemistry and the Molecular Biology Institute, University of California, Los Angeles, CA 90095 1569, USA
Nucleic Acids Res 40:1787-96. 2012.... - Cryptic transcription mediates repression of subtelomeric metal homeostasis genesIsabelle Toesca
Department of Chemistry and Biochemistry and the Molecular Biology Institute, University of California Los Angeles, Los Angeles, California, United States of America
PLoS Genet 7:e1002163. 2011.... - Sequential RNA degradation pathways provide a fail-safe mechanism to limit the accumulation of unspliced transcripts in Saccharomyces cerevisiaeShakir Sayani
Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California 90095, USA
RNA 18:1563-72. 2012..The presence of these two sequential degradation pathways for unspliced pre-mRNAs underscores the importance of limiting their accumulation and might serve as a fail-safe mechanism to prevent the expression of these nonfunctional RNAs... - Biochemical and genomic analysis of substrate recognition by the double-stranded RNA binding domain of yeast RNase IIIAnthony K Henras
Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095 1569, USA
RNA 11:1225-37. 2005..This study underscores the importance of a few amino acid contacts for positioning of a dsRBD onto its RNA target, and implicates the specific orientation of helix 1 on the RNA for proper positioning of the catalytic domain... - Contributions of Trf4p- and Trf5p-dependent polyadenylation to the processing and degradative functions of the yeast nuclear exosomeDefne E Egecioglu
Department of Chemistry and Biochemistry and the Molecular Biology Institute, University of California Los Angeles, Box 951569, Los Angeles, CA 90095 1569, USA
RNA 12:26-32. 2006..These results suggest that polyadenylation of RNA processing intermediates plays a functional role in RNA processing pathways and is not limited to RNA surveillance functions... - Nonsense-mediated mRNA decay mutes the splicing defects of spliceosome component mutationsTadashi Kawashima
Department of Chemistry and Biochemistry and the Molecular Biology Institute, University of California at Los Angeles, Los Angeles, California 90095 1569, USA
RNA 15:2236-47. 2009.... - Widespread impact of nonsense-mediated mRNA decay on the yeast intronomeShakir Sayani
Department of Chemistry and Biochemistry and the Molecular Biology Institute, University of California, Los Angeles, Los Angeles, CA 90095 1569, USA
Mol Cell 31:360-70. 2008..These results show that NMD has a wider impact than previously thought on the degradation of yeast-unspliced transcripts and plays an important role in discarding precursors of regulated or suboptimally spliced transcripts... - A conserved major groove antideterminant for Saccharomyces cerevisiae RNase III recognitionMui Sam
Department of Chemistry and Biochemistry and Molecular Biology Institute, Box 951569, University of California, Los Angeles, California 90095 1569, USA
Biochemistry 44:4181-7. 2005..cerevisiae RNase III activity, and suggest a rationale for their apparent counter selection in RNA processing sites... - Structural basis for recognition of the AGNN tetraloop RNA fold by the double-stranded RNA-binding domain of Rnt1p RNase IIIHaihong Wu
Department of Chemistry and Biochemistry and Molecular Biology Institute, University of California, Los Angeles, CA 90095-1569, USA
Proc Natl Acad Sci U S A 101:8307-12. 2004..These results show how a single dsRBD can convey specificity for particular RNA targets, by structure specific recognition of a conserved tetraloop fold... 
Sphingolipid signaling mediates iron toxicityYueh Jung Lee
Department of Chemistry and Biochemistry and the Molecular Biology Institute, University of California, Los Angeles, Los Angeles, CA 90095 1569, USA
Cell Metab 16:90-6. 2012..These results demonstrate an unexpected connection between sphingolipid flux and iron toxicity and show that activation of a signal transduction cascade contributes to iron-mediated cellular toxicity...- The Shq1p.Naf1p complex is required for box H/ACA small nucleolar ribonucleoprotein particle biogenesisPok Kwan Yang
Department of Chemistry and Biochemistry and the Molecular Biology Institute, UCLA, Los Angeles, California 90095 1569, USA
J Biol Chem 277:45235-42. 2002..Shq1p and Naf1p form a complex, but they are not strongly associated with box H/ACA snoRNPs. We propose that Shq1p and Naf1p are involved in the early biogenesis steps of box H/ACA snoRNP assembly... - RNAse III-mediated degradation of unspliced pre-mRNAs and lariat intronsMichal Danin-Kreiselman
Department of Chemistry and Biochemistry and the Molecular Biology Institute, University of California, Los Angeles, Box 951569, Los Angeles, CA 90095, USA
Mol Cell 11:1279-89. 2003..These results identify an alternative discard pathway for precursors and products of the splicing machinery and a physiological function for dsRNA in eukaryotic RNA catabolism... - The roles of endonucleolytic cleavage and exonucleolytic digestion in the 5'-end processing of S. cerevisiae box C/D snoRNAsChrissie Young Lee
Department of Chemistry and Biochemistry and the Molecular Biology Institute, University of California Los Angeles, Los Angeles, California 90095-1569, USA
RNA 9:1362-70. 2003.... - A cotranscriptional model for 3'-end processing of the Saccharomyces cerevisiae pre-ribosomal RNA precursorAnthony K Henras
Department of Chemistry and Biochemistry and the Molecular Biology Institute, University of California Los Angeles, Box 951569, Los Angeles, CA 90095-1569, USA
RNA 10:1572-85. 2004..These results show that a large fraction of Rnt1p is localized at the site of transcription of the rDNA, suggesting that the cleavage of the primary pre-rRNA transcript to generate the 35S pre-rRNA is a cotranscriptional event... - Structure and functional studies of the CS domain of the essential H/ACA ribonucleoparticle assembly protein SHQ1Mahavir Singh
Department of Chemistry and Biochemistry, UCLA, Los Angeles, California 90095, USA
J Biol Chem 284:1906-16. 2009..These results show that the CS domain is essential for Shq1p function in H/ACA snoRNP biogenesis in vivo, possibly in an Hsp90-independent manner... - Regulation and surveillance of normal and 3'-extended forms of the yeast aci-reductone dioxygenase mRNA by RNase III cleavage and exonucleolytic degradationCindy Zer
Department of Chemistry and Biochemistry and the Molecular Biology Institute, University of California, Los Angeles, CA 90095, USA
J Biol Chem 280:28997-9003. 2005.... - Cotranscriptional recruitment of the pseudouridylsynthetase Cbf5p and of the RNA binding protein Naf1p during H/ACA snoRNP assemblyPok Kwan Yang
Department of Chemistry and Biochemistry and Molecular Biology Institute, UCLA, Box 951569, Los Angeles, CA 90095-1569, USA
Mol Cell Biol 25:3295-304. 2005.... - The Chlamydomonas genome reveals the evolution of key animal and plant functionsSabeeha S Merchant
Department of Chemistry and Biochemistry, University of California at Los Angeles, Los Angeles, CA 90095, USA
Science 318:245-50. 2007.... - Multiple RNA surveillance pathways limit aberrant expression of iron uptake mRNAs and prevent iron toxicity in S. cerevisiaeAlbert Lee
Department of Chemistry and Biochemistry and the Molecular Biology Institute, University of California, Los Angeles, Box 951569, Los Angeles, California 90095, USA
Mol Cell 19:39-51. 2005....
Research Grants
- THE CONTROL OF GENE EXPRESSION BY EUKARYOTIC RNASE IIIGuillaume Chanfreau; Fiscal Year: 2009..These studies will contribute to our understanding of the roles of RNase III in the control of gene expression and of the mechanisms of adaptation of eukaryotic cell metabolism to iron deficiency. ..
- THE CONTROL OF GENE EXPRESSION BY EUKARYOTIC RNASE IIIGuillaume Chanfreau; Fiscal Year: 2007..These studies will contribute to our understanding of the roles of RNase III in the control of gene expression and of the mechanisms of adaptation of eukaryotic cell metabolism to iron deficiency. ..
- THE CONTROL OF GENE EXPRESSION BY EUKARYOTIC RNASE IIIGuillaume Chanfreau; Fiscal Year: 2004..In particular, provocative hypotheses suggesting that Rnt1p activity is modulated by N- terminal acetylation and that Rnt1p is involved in the sexual process will be tested. ..
- The Control of Gene Expression by Eukaryotic RibonucleasesGuillaume F Chanfreau; Fiscal Year: 2010..Our studies of the turnover of unspliced and mis-spliced RNAs will shed light on the mechanisms by which RNA degradation controls the expression of genes mutated in the context of a large number of genetic diseases. ..