Research Topics
Species | W L ChandlerSummaryAffiliation: University of Washington Country: USA Publications
| Collaborators
|
Detail Information
Publications
Effects of surgical trauma and cardiopulmonary bypass on active thrombin concentrations and the rate of thrombin inhibition in vivoTomas Velan
Department of Laboratory Medicine, University of Washington, Seattle, Wash. 98195-7110, USA
Pathophysiol Haemost Thromb 33:144-56. 2003..Both factors had returned to normal 2 h after surgery. We conclude that CPB with heparinization results in a rapid increase in thrombin inhibition leading to decreased active thrombin levels in vivo...
A community study of the effect of particulate matter on blood measures of inflammation and thrombosis in an elderly populationJeffrey H Sullivan
University of Washington, Department of Environmental and Occupational Health Sciences, Seattle, WA, USA
Environ Health 6:3. 2007..We also hypothesized that elevated PM would increase levels of cytokines in individuals with heart disease...- Plasmin generation and D-dimer formation during cardiopulmonary bypassWayne L Chandler
Department of Laboratory Medicine, University of Washington, Seattle, Washington 98104, USA
Blood Coagul Fibrinolysis 15:583-91. 2004.... - Elevated hemostatic factor levels as potential risk factors for thrombosisWayne L Chandler
Department of Laboratory Medicine, University of Washington, Seattle 98195, USA
Arch Pathol Lab Med 126:1405-14. 2002.... - Factor XIIIA and clot strength after cardiopulmonary bypassW L Chandler
Department of Laboratory Medicine, University of Washington, Seattle 98195, USA
Blood Coagul Fibrinolysis 12:101-8. 2001..Maintenance of adequate platelet counts and factor XIIIA levels at the end of CPB may play a role in maintaining clot strength and reducing blood loss... - Effects of hemodilution, blood loss, and consumption on hemostatic factor levels during cardiopulmonary bypassWayne L Chandler
Department of Laboratory Medicine, University of Washington, Harborview Medical Center, 325 9th Avenue, Seattle, WA 98104, USA
J Cardiothorac Vasc Anesth 19:459-67. 2005..The purpose of this study was to determine quantitatively the effects of consumption, hemodilution, and blood loss on coagulation and fibrinolytic factor levels during cardiopulmonary bypass... - Formation, inhibition and clearance of plasmin in vivoW L Chandler
Department of Laboratory Medicine, University of Washington, Seattle, Wash, USA
Haemostasis 30:204-18. 2000..The half-life of PAP was 4.5 +/- 1.6 h. In healthy subjects, the PAP level in plasma represents the average rate of plasminogen activation over the last 2-8 h... - Measurement of different forms of tissue plasminogen activator in plasmaW L Chandler
Department of Laboratory Medicine, University of Washington, Seattle, WA 98195, USA
Clin Chem 46:38-46. 2000..We evaluated assays to measure both total tissue plasminogen activator (tPA) and the three principle forms of tPA in plasma: active tPA, tPA complexed with plasminogen activator inhibitor type 1 (PAI-1), and tPA complexed with C1-inhibitor... 
Development of a rapid emergency hemorrhage panelWayne L Chandler
Department of Laboratory Medicine, University of Washington, Seattle, Washington 98195, USA
Transfusion 50:2547-52. 2010..Evaluation of hemostasis in bleeding patients requires both accuracy and speed...- Estimating the rate of thrombin and fibrin generation in vivo during cardiopulmonary bypassWayne L Chandler
Department of Laboratory Medicine, University of Washington, Seattle, WA 98195 7110, USA
Blood 101:4355-62. 2003.... - Reducing hemostatic activation during cardiopulmonary bypass: a combined approachMichael J Eisses
Department of Anesthesiology, University of Washington School of Medicine, Seattle, 98105, USA
Anesth Analg 98:1208-16, table of contents. 2004.... - The kinetics of plasmin inhibition by aprotinin in vivoHyoung-Min Kang
Department of Laboratory Medicine, Box 357110, University of Washington, Seattle, WA 98195-7110, USA
Thromb Res 115:327-40. 2005..CONCLUSIONS: The rise in plasmin generation and fibrin degradation that occurs during standard CPB is suppressed by the addition of aprotinin, which returns the patient to near baseline fibrin degradation rates during CPB... - Strategies to reduce hemostatic activation during cardiopulmonary bypassMichael J Eisses
Department of Anesthesiology, Children's Hospital and Regional Medical Center, University of Washington, Seattle, USA
Thromb Res 117:689-703. 2006..Kinetic modeling of hemostasis in individual patients showed that a modified CPB protocol could reduce excessive thrombin generation during CPB and suppress fibrin degradation moving hemostatic regulation back towards normal... - Cardiopulmonary bypass parameters and hemostatic response to cardiopulmonary bypass in infants versus childrenMichael J Eisses
Department of Anesthesiology and Pain Medicine, University of Washington School of Medicine, Seattle, WA, USA
J Cardiothorac Vasc Anesth 22:53-9. 2008.... - Clearance of tissue plasminogen activator (TPA) and TPA/plasminogen activator inhibitor type 1 (PAI-1) complex: relationship to elevated TPA antigen in patients with high PAI-1 activity levelsW L Chandler
Department of Laboratory Medicine, University of Washington, Seattle 98195 7110, USA
Circulation 96:761-8. 1997.... - Does the type of surgery effect systemic response following cardiopulmonary bypass?Hiroo Takayama
Department of Surgery, Division of Cardiothoracic Surgery, University of Washington School of Medicine, Seattle, Washington 98195, USA
J Card Surg 22:307-13. 2007..We hypothesized that valve surgery induces more prominent systemic reaction compared to isolated on-pump CABG... - Prothrombotic coagulation abnormalities preceding the hemolytic-uremic syndromeWayne L Chandler
Department of Laboratory Medicine, University of Washington School of Medicine, Seattle 98105, USA
N Engl J Med 346:23-32. 2002..80; P<0.001). CONCLUSIONS: In the hemolytic-uremic syndrome, thrombin generation (probably due to accelerated thrombogenesis) and inhibition of fibrinolysis precede renal injury and may be the cause of such injury... - Effect of polymer coating (poly 2-methoxyethylacrylate) of the oxygenator on hemostatic markers during cardiopulmonary bypass in childrenMichael J Eisses
Department of Anesthesiology and Pain Medicine, University of Washington School of Medicine, Seattle, WA 98105, USA
J Cardiothorac Vasc Anesth 21:28-34. 2007.... - Thrombogenic alleles, Escherichia coli O157:H7 infections, and hemolytic uremic syndromeJ T Sprouse
Department of Laboratory Medicine, University of Washington School of Medicine, Seattle, Washington, USA
Blood Coagul Fibrinolysis 12:283-8. 2001..Serum PAI-1 levels did not correlate with the PAI-1 genotype. We conclude that the alleles studied are not major risk factors for the acquisition of E. coli O157:H7 infection, or of E. coli O157:H7-related HUS... - Activation, aggregation and adhesion of platelets exposed to high-intensity focused ultrasoundS L Poliachik
Center for Industrial and Medical Ultrasound, Applied Physics Laboratory, University of Washington, 1013 NE 430th Street, Seattle, WA 98105, USA
Ultrasound Med Biol 27:1567-76. 2001..Regression analysis shows a weak correlation (r(2) = 0.11) between aggregation and ultrasound intensity, but a substantial correlation (r(2) = 0.76) between aggregation and cavitation occurrence... - Coagulation factor VII gene haplotypes, obesity-related traits, and cardiovascular risk in young womenA P Reiner
Department of Epidemiology, University of Washington, Seattle, WA, USA
J Thromb Haemost 5:42-9. 2007..Associations between common F7 haplotypes, plasma factor VII (FVII) levels, and cardiovascular risk have recently been reported in population studies involving predominantly European men... - Heritability of C-reactive protein and association with apolipoprotein E genotypes in Japanese AmericansM A Austin
Department of Epidemiology, School of Public Health and Community Medicine, Box 357236, University of Washington, Seattle, WA 98195 7236, USA
Ann Hum Genet 68:179-88. 2004..07). These results demonstrate the presence of both genetic and environmental effects on CRP levels among Asian Americans, and additional studies are needed to determine if the APOE gene contributes to these genetic influences... - The relation between cavitation and platelet aggregation during exposure to high-intensity focused ultrasoundSandra L Poliachik
Center for Industrial and Medical Ultrasound, Applied Physics Laboratory, University of Washington, Seattle, USA
Ultrasound Med Biol 30:261-9. 2004..Our results show that increased cavitation activity lowers the intensity threshold to produce platelet aggregation and decreased cavitation activity in the overpressure system raises the intensity threshold for platelet aggregation... - Heritability of multivariate factors of the metabolic syndrome in nondiabetic Japanese americansMelissa A Austin
Department of Epidemiology and Institute for Public Health Genetics, School of Public Health and Community Medicine, University of Washington, Seattle, 98195 7236, USA
Diabetes 53:1166-9. 2004..25, P = 0.026). This analysis shows that independent, multivariate factors of the metabolic syndrome are heritable, demonstrating genetic influences on the underlying pathophysiological mechanisms of the syndrome... - Effect of valproate on hemostatic function in patients with traumatic brain injuryGail D Anderson
Department of Pharmacy, University of Washington, Box 357630, Seattle, WA 98195, USA
Epilepsy Res 57:111-9. 2003..To evaluate whether valproate (VPA) resulted in laboratory or clinical evidence of a change in bleeding-related measures associated with VPA therapy in patients sustaining a traumatic brain injury... - Secretion of tissue plasminogen activator and plasminogen activator inhibitor 1 during cardiopulmonary bypassWayne L Chandler
Department of Laboratory Medicine, University of Washington, Box 357110, Seattle, WA 98195 7110, USA
Thromb Res 112:185-92. 2003..The goal of this study was to estimate the rate of t-PA and PAI-1 secretion in vivo, before, during and after CPB... - Venous thromboembolism in trauma: a local manifestation of systemic hypercoagulability?Mark H Meissner
Department of Surgery, Harborview Medical Center, University of Washington School of Medicine, Seattle, 98195, USA
J Trauma 54:224-31. 2003..The purpose of this study was to evaluate the relative importance of systemic hypercoagulability, preexisting and acquired risk factors, and specific injury patterns in the development of venous thromboembolism (VTE) after injury... - Limitation of thrombin generation, platelet activation, and inflammation by elimination of cardiotomy suction in patients undergoing coronary artery bypass grafting treated with heparin-bonded circuitsGabriel S Aldea
Division of Cardiothoracic Surgery, Department of Surgery, University of Washington School of Medicine, Seattle, WA 98195 3166, USA
J Thorac Cardiovasc Surg 123:742-55. 2002..A prospective, randomized study was undertaken to evaluate the specific effects of cardiotomy suction... - Coagulation, fibrinolysis, and recanalization after acute deep venous thrombosisMark H Meissner
Department of Surgery, University of Washington School of Medicine, Seattle, USA
J Vasc Surg 35:278-85. 2002..The purpose of this study was to examine the relationship between recanalization and plasma markers of coagulation and fibrinolysis... - Thrombin generation in trauma patientsNancy M Dunbar
Department of Laboratory Medicine, University of Washington, Seattle, Washington 98195, USA
Transfusion 49:2652-60. 2009..ACT is incompletely understood, but is similar to disseminated intravascular coagulation (DIC) and is associated with poor outcome... - Lack of depression effect on platelet activation in patients with heart failureJagoda Pasic
Department of Psychiatry and Behavioral Sciences, University of Washington, Harborview Medical Center, 325 Ninth Avenue, Box 350896, Seattle, WA 98104 2499, USA
Psychiatry Res 129:289-92. 2004..Our data show no group differences in P-selectin values, which suggests that comorbid depression has no additive effect on platelet activation in CHF patient... - Comparison of the lipoprotein, carbohydrate, and hemostatic effects of phasic oral contraceptives containing desogestrel or levonorgestrelR H Knopp
Northwest Lipid Research Clinic in the Department of Medicine, University of Washington, Seattle, USA
Contraception 63:1-11. 2001..The lipoprotein, SHBG, and protein S and factor V differences are likely due to the lesser androgenicity of DSG allowing for a greater expression of the dose of estrogen... - The circulatory regulation of TPA and UPA secretion, clearance, and inhibition during exercise and during the infusion of isoproterenol and phenylephrineW L Chandler
Department of Laboratory Medicine, University of Washington, Seattle 98195, USA
Circulation 92:2984-94. 1995..Exercise to exhaustion and infusions of isoproterenol and phenylephrine were used to study interactions between plasminogen activator regulation and the control of regional blood flow in 10 healthy males... - Failure of peripheral arterial thrombolysis due to elevated plasminogen activator inhibitor type 1Stephen C Nicholls
Division of Vascular Surgery, Department of Surgery, Harborview Medical Center, University of Washington, Seattle, Washington 98104, USA
Blood Coagul Fibrinolysis 14:729-33. 2003..We conclude that elevated PAI-1 during acute peripheral arterial thrombolysis is associated with an increased risk of lysis failure due to reduced levels of circulating active t-PA or urokinase... - More on: calibration for the measurement of microparticles: the authors respondW L Chandler
Department of Laboratory Medicine, University of Washington, Seattle, WA, USA
J Thromb Haemost 9:1681-2. 2011..This issue, pp 1679-81.DOI: 10.1111/j.1538-7836.2011.04384.x... - A new microparticle size calibration standard for use in measuring smaller microparticles using a new flow cytometerW L Chandler
Department of Laboratory Medicine, University of Washington, Seattle, WA 98195, USA
J Thromb Haemost 9:1216-24. 2011..Microparticle size measurements are often calibrated on flow cytometers using polystyrene microspheres that forward scatter more light vs. particle diameter than cellular microparticles... - False negative factor V Leiden assay following allogeneic stem cell transplantK P Crookston
Department of Laboratory Medicine, University of Washington, Seattle 98195, USA
Br J Haematol 100:600-2. 1998..The patient had undergone a stem cell transplant several months before, and the leucocytes in her blood were derived from the stem cell donor, which lacked the FV defect... - Comparing the prothrombin time INR versus the APTT to evaluate the coagulopathy of acute traumaShan Yuan
Department of Laboratory Medicine, Box 357110, University of Washington, Seattle, WA 98195 7110, USA
Thromb Res 120:29-37. 2007..In trauma patients, PT/INR or aPTT cutoffs of > or =1.5x normal are often used as triggers for the transfusion of plasma... - Ventricular cerebrospinal fluid hypocretin-1 inversely correlates with glucose levels in cerebrospinal fluid and serum from patients with neurological injuriesMichael J Doherty
Swedish Epilepsy Center, Swedish Medical Center, Seattle, WA, USA
Neurocrit Care 3:230-3. 2005.... - Cerebrospinal fluid creatine kinase-BB isoenzyme activity and outcome after subarachnoid hemorrhageW M Coplin
Department of Neurology, School of Medicine, University of Washington, Seattle, USA
Arch Neurol 56:1348-52. 1999..Results of previous studies have shown that CK-BB can be detected in the CSF of patients with aneurysmal subarachnoid hemorrhage (SAH), but whether CK-BB levels correlate with patients' neurologic outcomes is unknown... - Monitoring direct thrombin inhibitors with a plasma diluted thrombin timeJason E Love
Department of Laboratory Medicine, Box 357110, University of Washington, Seattle, WA 98195, USA
Thromb Haemost 98:234-42. 2007..In conclusion, the plasma diluted thrombin time appears to be a viable alternative to the aPTT for monitoring DTI levels, especially in patients with lupus inhibitors or low levels of vitamin K-dependent factors... - Comparison of three methods for measuring factor VIII levels in plasmaWayne L Chandler
Dept of Laboratory Medicine, Box 357110, University of Washington, Seattle, WA 98195, USA
Am J Clin Pathol 120:34-9. 2003.... - Procoagulant activity in trauma patientsWayne L Chandler
Dept of Laboratory Medicine, University of Washington, Seattle, 98195, USA
Am J Clin Pathol 134:90-6. 2010..Tissue factor activity was 3- to 4-fold higher in trauma patients with coagulopathy vs patients without coagulopathy (P = .002). Trauma patients with coagulopathy have increased circulating tissue factor activity... 
Adipokines, inflammation, and visceral adiposity across the menopausal transition: a prospective studyChristine G Lee
Department of Medicine, Division of Metabolism, Endocrinology, and Nutrition, University of Washington, Seattle, Washington 98195 4626, USA
J Clin Endocrinol Metab 94:1104-10. 2009..Postmenopausal women have greater visceral adiposity compared with premenopausal women. Adipokines are associated with increased adiposity, insulin resistance, and atherosclerosis...- Cardiac ischemia during hemolytic uremic syndromeMeena Thayu
Department of Pediatrics, University of Washington School of Medicine, Seattle, Washington, USA
Pediatr Nephrol 18:286-9. 2003..It is possible that myocardial ischemia and secondary arrhythmias account for some sudden deaths that occur during acute HUS... - Comparison of plasma with whole blood prothrombin time and fibrinogen on the same instrumentTIMOTHY K AMUKELE
Department of Laboratory Medicine, University of Washington, Seattle, USA
Am J Clin Pathol 133:550-6. 2010..Accuracy is limited by variations in hematocrit with worsening accuracy for samples with high INRs or low fibrinogen levels... - Optimization of plasma fluorogenic thrombin-generation assaysWayne L Chandler
Department of Laboratory Medicine, Box 357110, University of Washington, Seattle, WA 98195, USA
Am J Clin Pathol 132:169-79. 2009.... - Quality assurance in hemostasis: the perspective from the College of American Pathologists proficiency testing programMark T Cunningham
Department of Pathology, University of Kansas Medical Center, Kansas City, Kansas 66160, USA
Semin Thromb Hemost 33:250-8. 2007.... - Laboratory reporting of the international normalized ratio: progress and problemsJohn D Olson
Department of Pathology, University of Texas Health Science Center, San Antonio, TX 78229 3900, USA
Arch Pathol Lab Med 131:1641-7. 2007..The international normalized ratio (INR) is widely used to monitor oral anticoagulation and to evaluate patients with coagulation disorders... - Thrombotic microangiopathiesPhillip I Tarr
N Engl J Med 347:2171-3; author reply 2171-3. 2002 - Effect of fondaparinux on coagulation assays: results of College of American Pathologists proficiency testingAgata Smogorzewska
Department of Pathology and Laboratory Medicine, Harvard Medical School, Massachusetts General Hospital, Boston 02114, USA
Arch Pathol Lab Med 130:1605-11. 2006..A fondaparinux standard curve should be used for reporting fondaparinux levels using an anti-factor Xa assay... - Antithrombin reduces monocyte and neutrophil CD11b up regulation in addition to blocking platelet activation during extracorporeal circulationChristine S Rinder
Department of Anesthesiology, Yale University School of Medicine, New Haven, Connecticut 06520 8051, USA
Transfusion 46:1130-7. 2006.... - The effect of submaximal exercise on fibrinolysisZlatko Fras
University Medical Center Ljubljana, Department for Vascular Medicine, Ljubljana, Slovenia
Blood Coagul Fibrinolysis 15:227-34. 2004.... - Coagulation analyzersKandice Kottke-Marchant
Department of Clinical Pathology, Cleveland Clinic Foundation, USA
CAP Today 17:18-22, 26-8, 30-3. 2003