Nadja B Cech

Summary

Affiliation: University of North Carolina at Greensboro
Country: USA

Publications

  1. pmc Quorum quenching and antimicrobial activity of goldenseal (Hydrastis canadensis) against methicillin-resistant Staphylococcus aureus (MRSA)
    Nadja B Cech
    Department of Chemistry Biochemistry, The University of North Carolina Greensboro, Greensboro, NC, USA
    Planta Med 78:1556-61. 2012
  2. ncbi request reprint High performance liquid chromatography/electrospray ionization mass spectrometry for simultaneous analysis of alkamides and caffeic acid derivatives from Echinacea purpurea extracts
    Nadja B Cech
    Department of Chemistry and Biochemistry, University of North Carolina Greensboro, 435 New Science Bldg, Greensboro, NC 27402, USA
    J Chromatogr A 1103:219-28. 2006
  3. ncbi request reprint Liver enzyme-mediated oxidation of Echinacea purpurea alkylamides: production of novel metabolites and changes in immunomodulatory activity
    Nadja B Cech
    Department of Chemistry and Biochemistry, The University of North Carolina Greensboro, Greensboro, NC 27402, USA
    Planta Med 72:1372-7. 2006
  4. doi request reprint Echinacea and its alkylamides: effects on the influenza A-induced secretion of cytokines, chemokines, and PGE₂ from RAW 264.7 macrophage-like cells
    Nadja B Cech
    Department of Chemistry and Biochemistry, The University of North Carolina Greensboro, Box 26170, Greensboro, NC 27402, USA
    Int Immunopharmacol 10:1268-78. 2010
  5. doi request reprint Comparison of electrospray ionization and atmospheric pressure photoionization liquid chromatography mass spectrometry methods for analysis of ergot alkaloids from endophyte-infected sleepygrass (Achnatherum robustum)
    Alan K Jarmusch
    Department of Chemistry and Biochemistry, University of North Carolina Greensboro, PO Box 26170, Greensboro, NC 27402, United States
    J Pharm Biomed Anal 117:11-7. 2016
  6. pmc Quantitative analysis of autoinducing peptide I (AIP-I) from Staphylococcus aureus cultures using ultrahigh performance liquid chromatography-high resolving power mass spectrometry
    Hiyas A Junio
    Department of Chemistry Biochemistry, The University of North Carolina Greensboro, 435 Sullivan Bldg, Greensboro, NC 27402, USA
    J Chromatogr B Analyt Technol Biomed Life Sci 930:7-12. 2013
  7. pmc Synergy-directed fractionation of botanical medicines: a case study with goldenseal (Hydrastis canadensis)
    Hiyas A Junio
    Department of Chemistry and Biochemistry, The University of North Carolina Greensboro, P O Box 26170, Greensboro, North Carolina 27402, USA
    J Nat Prod 74:1621-9. 2011
  8. pmc Polyhydroxyanthraquinones as quorum sensing inhibitors from the guttates of Penicillium restrictum and their analysis by desorption electrospray ionization mass spectrometry
    Mario Figueroa
    Department of Chemistry and Biochemistry, University of North Carolina at Greensboro, Greensboro, North Carolina 27402, United States
    J Nat Prod 77:1351-8. 2014
  9. pmc Sarothrin from Alkanna orientalis is an antimicrobial agent and efflux pump inhibitor
    Jessica R Bame
    Department of Chemistry and Biochemistry, The University of North Carolina Greensboro, Greensboro, NC, USA
    Planta Med 79:327-9. 2013
  10. pmc Goldenseal (Hydrastis canadensis L.) extracts synergistically enhance the antibacterial activity of berberine via efflux pump inhibition
    Keivan A Ettefagh
    Department of Chemistry Biochemistry, The University of North Carolina Greensboro, Greensboro, NC 27402, USA
    Planta Med 77:835-40. 2011

Collaborators

  • Kevin Spelman
  • Nicholas H Oberlies
  • Stanley H Faeth
  • G M Raner
  • Dean Gray
  • Tamam El-Elimat
  • Brandie M Ehrmann
  • Hiyas A Junio
  • Keivan A Ettefagh
  • Daniel A Todd
  • Tyler N Graf
  • Alan K Jarmusch
  • Tatsiana Shymanovich
  • Mario Figueroa
  • Emily R Britton
  • Huzefa A Raja
  • Scott A Jarmusch
  • Alexander R Horswill
  • Joshua J Kellogg
  • Ashleigh M Musso
  • Susanna Saari
  • Mary E Lovin
  • Joseph M Egan
  • Adam R Brown
  • Clint M Alfaro
  • Robert O Bussey
  • Kaira M Wagoner
  • Joseph O Falkinham
  • Jeffrey S Kavanaugh
  • Glenn W Kaatz
  • Jessica R Bame
  • Karen M Vandermolen
  • Arlene A Sy-Cordero
  • Johnna T Burns
  • Stacy S Bae
  • Masa Sasagawa
  • David E Nichols
  • Olav M Kvalheim
  • Miranda J Weavil
  • Daniel H Foil
  • Martha Leyte-Lugo
  • Scott M Laster
  • Monika Juzumaite
  • Laura F Grubbs
  • Martina Oberhofer
  • Daniel Todd
  • Nikki D Charlton
  • Bryan D Schindler
  • Patrick S Cole
  • Carolyn A Young
  • Carolyn B Ibberson
  • Travis V Gulledge
  • Ashley N Moody
  • Diana L Huestis
  • R Graham Cooks
  • Agbo Oma Uwakweh
  • Tovi Lehmann
  • Fredrick S Carter
  • Gideon Wasserberg
  • Richard A Cech
  • Cedric J Pearce
  • Mary F Paine
  • R Owen Bussey
  • Kathryn T Micko
  • Scott J Richter
  • Robert E Cannon
  • Cynthia A Wenner
  • Gary W Elmer

Detail Information

Publications26

  1. pmc Quorum quenching and antimicrobial activity of goldenseal (Hydrastis canadensis) against methicillin-resistant Staphylococcus aureus (MRSA)
    Nadja B Cech
    Department of Chemistry Biochemistry, The University of North Carolina Greensboro, Greensboro, NC, USA
    Planta Med 78:1556-61. 2012
    ..These findings lend support for the traditional application of crude H. canadensis extracts in the prevention of infection...
  2. ncbi request reprint High performance liquid chromatography/electrospray ionization mass spectrometry for simultaneous analysis of alkamides and caffeic acid derivatives from Echinacea purpurea extracts
    Nadja B Cech
    Department of Chemistry and Biochemistry, University of North Carolina Greensboro, 435 New Science Bldg, Greensboro, NC 27402, USA
    J Chromatogr A 1103:219-28. 2006
    ..purpurea extracts that have been stored for 18 months indicate that they contain caftaric acid, cichoric acid, and undeca-2Z,4E-diene-8,10-diynoic acid isobutylamide at concentrations of 0.7, 0.71 and 2.0mg/mL, respectively...
  3. ncbi request reprint Liver enzyme-mediated oxidation of Echinacea purpurea alkylamides: production of novel metabolites and changes in immunomodulatory activity
    Nadja B Cech
    Department of Chemistry and Biochemistry, The University of North Carolina Greensboro, Greensboro, NC 27402, USA
    Planta Med 72:1372-7. 2006
    ..These findings highlight the importance of considering the influence of liver enzyme metabolism when evaluating the immunomodulatory effects of alkylamides...
  4. doi request reprint Echinacea and its alkylamides: effects on the influenza A-induced secretion of cytokines, chemokines, and PGE₂ from RAW 264.7 macrophage-like cells
    Nadja B Cech
    Department of Chemistry and Biochemistry, The University of North Carolina Greensboro, Box 26170, Greensboro, NC 27402, USA
    Int Immunopharmacol 10:1268-78. 2010
    ....
  5. doi request reprint Comparison of electrospray ionization and atmospheric pressure photoionization liquid chromatography mass spectrometry methods for analysis of ergot alkaloids from endophyte-infected sleepygrass (Achnatherum robustum)
    Alan K Jarmusch
    Department of Chemistry and Biochemistry, University of North Carolina Greensboro, PO Box 26170, Greensboro, NC 27402, United States
    J Pharm Biomed Anal 117:11-7. 2016
    ..143±0.051 ppm and 0.2822±0.0071 ppm, respectively. There was no statistically significant difference between these concentrations and those determined using ESI for the same samples...
  6. pmc Quantitative analysis of autoinducing peptide I (AIP-I) from Staphylococcus aureus cultures using ultrahigh performance liquid chromatography-high resolving power mass spectrometry
    Hiyas A Junio
    Department of Chemistry Biochemistry, The University of North Carolina Greensboro, 435 Sullivan Bldg, Greensboro, NC 27402, USA
    J Chromatogr B Analyt Technol Biomed Life Sci 930:7-12. 2013
    ..Finally, it was determined that virulence of S. aureus was correlated with AIP-I production in some (but not all) strains analyzed...
  7. pmc Synergy-directed fractionation of botanical medicines: a case study with goldenseal (Hydrastis canadensis)
    Hiyas A Junio
    Department of Chemistry and Biochemistry, The University of North Carolina Greensboro, P O Box 26170, Greensboro, North Carolina 27402, USA
    J Nat Prod 74:1621-9. 2011
    ..canadensis roots. Thus, it may be possible to produce an extract with optimal activity against S. aureus using a combination of goldenseal roots and leaves...
  8. pmc Polyhydroxyanthraquinones as quorum sensing inhibitors from the guttates of Penicillium restrictum and their analysis by desorption electrospray ionization mass spectrometry
    Mario Figueroa
    Department of Chemistry and Biochemistry, University of North Carolina at Greensboro, Greensboro, North Carolina 27402, United States
    J Nat Prod 77:1351-8. 2014
    ....
  9. pmc Sarothrin from Alkanna orientalis is an antimicrobial agent and efflux pump inhibitor
    Jessica R Bame
    Department of Chemistry and Biochemistry, The University of North Carolina Greensboro, Greensboro, NC, USA
    Planta Med 79:327-9. 2013
    ..orientalis. Our findings suggest that the effectiveness of A. orientalis extracts is due to a combination of multiple constituents, including sarothrin...
  10. pmc Goldenseal (Hydrastis canadensis L.) extracts synergistically enhance the antibacterial activity of berberine via efflux pump inhibition
    Keivan A Ettefagh
    Department of Chemistry Biochemistry, The University of North Carolina Greensboro, Greensboro, NC 27402, USA
    Planta Med 77:835-40. 2011
    ..The three most abundant H. canadensis alkaloids, berberine, hydrastine, and canadine, are not responsible for the efflux pump inhibitory activity of the extracts from H. canadensis aerial portions...
  11. pmc Effects of herbal products and their constituents on human cytochrome P450(2E1) activity
    Gregory M Raner
    The University of North Carolina at Greensboro, Department of Chemistry and Biochemistry, 414 Science Building, Greensboro, NC 27402, USA
    Food Chem Toxicol 45:2359-65. 2007
    ..Commercial green tea preparations, along with four of the individual tea catechins, were also examined and were found to have no effect on the activity of P450(2E1)...
  12. pmc Comparison of alkylamide yield in ethanolic extracts prepared from fresh versus dry Echinacea purpurea utilizing HPLC-ESI-MS
    Kevin Spelman
    Department of Chemistry and Biochemistry, The University of North Carolina Greensboro, P O Box 26170, Greensboro, NC 27402, USA
    J Pharm Biomed Anal 49:1141-9. 2009
    ..These results demonstrate the differences of the described extractions and utility of the analytical methods used to determine the wide-ranging individual alkylamide content of commonly consumed Echinacea extracts...
  13. doi request reprint Role for PPARgamma in IL-2 inhibition in T cells by Echinacea-derived undeca-2E-ene-8,10-diynoic acid isobutylamide
    Kevin Spelman
    University of North Carolina Greensboro, Department of Chemistry and Biochemistry, P O Box 26170, Greensboro, NC 27405, USA
    Int Immunopharmacol 9:1260-4. 2009
    ..These experiments demonstrate that PPARgamma is involved in T cell IL-2 inhibition by undeca-2-ene-8,10-diynoic acid and suggest that cytokine modulation by the alkylamides is due to polyvalent activity...
  14. doi request reprint Biochemometrics for Natural Products Research: Comparison of Data Analysis Approaches and Application to Identification of Bioactive Compounds
    Joshua J Kellogg
    Department of Chemistry and Biochemistry, University of North Carolina Greensboro, Greensboro, North Carolina 27402, United States
    J Nat Prod 79:376-86. 2016
    ..A benefit of this approach is the ability to integrate multiple stages of fractionation and bioassay data into a single analysis. ..
  15. doi request reprint Flavonolignans from Aspergillus iizukae, a fungal endophyte of milk thistle (Silybum marianum)
    Tamam El-Elimat
    Department of Chemistry and Biochemistry, University of North Carolina at Greensboro, Greensboro, North Carolina 27402, United States
    J Nat Prod 77:193-9. 2014
    ..marianum were added. The cycle of attenuation followed by resumed biosynthesis of these flavonolignans was replicated in triplicate. ..
  16. pmc Antimycobacterial furofuran lignans from the roots of Anemopsis californica
    Robert O Bussey
    Department of Chemistry and Biochemistry, University of North Carolina Greensboro, Greensboro, NC, USA
    Planta Med 80:498-501. 2014
    ..This paper is the first to report the isolation of these compounds from a member of the Saururaceae family, and the first to describe their activity against nontuberculous mycobacteria. ..
  17. pmc High-resolution MS, MS/MS, and UV database of fungal secondary metabolites as a dereplication protocol for bioactive natural products
    Tamam El-Elimat
    Department of Chemistry and Biochemistry, University of North Carolina at Greensboro, P O Box 26170, Greensboro, North Carolina 27402, United States
    J Nat Prod 76:1709-16. 2013
    ..The specific attributes of this dereplication methodology include a focus on bioactive secondary metabolites from fungi, the use of a 10 min chromatographic method, and the inclusion of both HRMS and MS/MS data. ..
  18. doi request reprint A mass spectrometry-based assay for improved quantitative measurements of efflux pump inhibition
    Adam R Brown
    Department of Chemistry Biochemistry, The University of North Carolina Greensboro, Greensboro, North Carolina, United States of America
    PLoS ONE 10:e0124814. 2015
    ..The assay described herein could be useful in future screening efforts to identify efflux pump inhibitors, particularly in situations where optical interference precludes the application of methods that rely on fluorescence. ..
  19. doi request reprint Alkaloid variation among epichloid endophytes of sleepygrass (Achnatherum robustum) and consequences for resistance to insect herbivores
    Tatsiana Shymanovich
    Department of Biology, University of North Carolina Greensboro, 312 Eberhart Bldg, Greensboro, NC, 27412, USA
    J Chem Ecol 41:93-104. 2015
    ..The results of this study suggest that alkaloids produced by the Cloudcroft endophyte, specifically ergonovine, have insecticidal properties. ..
  20. doi request reprint A validated liquid chromatography-electrospray ionization-mass spectrometry method for quantification of spilanthol in Spilanthes acmella (L.) Murr
    Stacy S Bae
    Department of Chemistry and Biochemistry, The University of North Carolina Greensboro, 435 Sullivan Science Bldg, 301 McIver St, Greensboro, NC 27412, USA
    Phytochem Anal 21:438-43. 2010
    ..Recently this plant has been of interest due to its potential for the treatment and prevention of malaria...
  21. doi request reprint A new mass spectrometry based bioassay for the direct assessment of hyaluronidase activity and inhibition
    Emily R Britton
    Department of Chemistry and Biochemistry, University of North Carolina at Greensboro, Greensboro, NC, USA
    J Microbiol Methods 119:163-7. 2015
    ..4±9.8 μM. In conclusion, we describe a new, direct, and reproducible method for assessing hyaluronidase activity using mass spectrometry that can facilitate the discovery of inhibitors...
  22. doi request reprint Investigations of analyte-specific response saturation and dynamic range limitations in atmospheric pressure ionization mass spectrometry
    Clint M Alfaro
    Department of Chemistry and Biochemistry, University of North Carolina at Greensboro, P O Box 26170, Greensboro, North Carolina 27402, United States
    Anal Chem 86:10639-45. 2014
    ..Among the sources investigated herein, the HESI source achieved the best analytical performance (widest linear dynamic range, lowest LOD), but the APCI source was less subject to saturation in response at high concentration. ..
  23. pmc Ethanolic Echinacea purpurea Extracts Contain a Mixture of Cytokine-Suppressive and Cytokine-Inducing Compounds, Including Some That Originate from Endophytic Bacteria
    Daniel A Todd
    Department of Chemistry and Biochemistry, University of North Carolina at Greensboro, Greensboro, North Carolina, United States of America
    PLoS ONE 10:e0124276. 2015
    ..purpurea extracts can originate from endophytic bacteria. Together, our findings indicate that ethanolic E. purpurea extracts contain multiple constituents that differentially regulate cytokine production by macrophages. ..
  24. pmc Rapid Quantitation of Furanocoumarins and Flavonoids in Grapefruit Juice using Ultra-Performance Liquid Chromatography
    Karen M Vandermolen
    Department of Chemistry and Biochemistry, The University of North Carolina at Greensboro, Patricia A Sullivan Science Building, PO Box 26170, Greensboro, NC, 27402, USA
    Phytochem Anal 24:654-60. 2013
    ..The potential for drug interactions with a food product necessitates an understanding of the expected concentrations of a suite of structurally diverse and potentially bioactive compounds...
  25. pmc Identification of morphological and chemical markers of dry- and wet-season conditions in female Anopheles gambiae mosquitoes
    Kaira M Wagoner
    Department of Biology, University of North Carolina Greensboro, 235 Eberhart Bldg, Greensboro, NC 27402, USA
    Parasit Vectors 7:294. 2014
    ..In this study, we examined the effect of photoperiod and relative humidity on morphologic and chemical traits known to control water loss in mosquitoes...
  26. ncbi request reprint Echinacea alkylamides inhibit interleukin-2 production by Jurkat T cells
    Masa Sasagawa
    Bastyr University, Department of Basic Sciences, School of Natural Health Sciences, Kenmore, WA 98028, USA
    Int Immunopharmacol 6:1214-21. 2006
    ..Thus, alkylamides present in E. purpurea suppress the ability of activated Jurkat T cells to produce IL-2 independently of direct, cytotoxic effects...