Research Topics
Species | E L CavalieriSummaryAffiliation: University of Nebraska Medical Center Country: USA Publications
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Publications
Ortho-quinones of benzene and estrogens induce hyperproliferation of human peripheral blood mononuclear cellsDhrubajyoti Chakravarti
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, NE 68198, USA
Leuk Lymphoma 47:2635-44. 2006..Thus, DNA damage induced by the ortho-quinones of benzene and estradiol may promote the growth of human blood mononuclear cells, including those that appear in large numbers in leukemia and lymphoma...- Novel biomarkers for risk of prostate cancer: results from a case-control studyLi Yang
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, Nebraska 68198 6805, USA
Prostate 69:41-8. 2009..The goal of this case-control study was to detect and identify potential biomarkers of Pca... - Molecular origin of cancer: catechol estrogen-3,4-quinones as endogenous tumor initiatorsE L Cavalieri
Eppley Institute for Research in Cancer, University of Nebraska Medical Center, 600 South 42nd Street, Omaha, NE 68198 6805, USA
Proc Natl Acad Sci U S A 94:10937-42. 1997..4 micromol of adduct/mol DNA-phosphate. In each case, the level of stable adducts was negligible. These results, complemented by other data, strongly support the hypothesis that CE-3,4-Q are endogenous tumor initiators... - A unifying mechanism in the initiation of cancer and other diseases by catechol quinonesErcole L Cavalieri
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, NE 68198 6805, USA
Ann N Y Acad Sci 1028:247-57. 2004..In summary, the apurinic sites produced in DNA from the loss of these depurinating adducts can be converted into mutations by error-prone repair, which may initiate cancer and other diseases... - Catechol estrogen quinones as initiators of breast and other human cancers: implications for biomarkers of susceptibility and cancer preventionErcole Cavalieri
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, NE 68198 6805, USA
Biochim Biophys Acta 1766:63-78. 2006..Initiated cells may be promoted by a number of processes, including hormone receptor stimulated proliferation. These results lay the groundwork for assessing risk and preventing disease... - Catechol quinones of estrogens in the initiation of breast, prostate, and other human cancers: keynote lectureErcole Cavalieri
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, NE 8198 6805, USA
Ann N Y Acad Sci 1089:286-301. 2006..This unifying mechanism of the origin of cancer and other diseases suggests preventive strategies based on the level of depurinating DNA adducts that generate the first critical step in the initiation of diseases... - Mechanism of DNA depurination by carcinogens in relation to cancer initiationErcole Cavalieri
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, NE 68198 6805, USA
IUBMB Life 64:169-79. 2012..Understanding why the depurinating estrogen-DNA adducts come from DNA, and not from RNA, underscores the critical role that these adducts play in initiating cancer... - Catechol ortho-quinones: the electrophilic compounds that form depurinating DNA adducts and could initiate cancer and other diseasesErcole L Cavalieri
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, NE 68198 6805, USA
Carcinogenesis 23:1071-7. 2002..These data suggest that there is a unifying molecular mechanism, namely, formation of specific depurinating DNA adducts at the N-7 of guanine and N-3 of adenine, that could initiate many cancers and neurodegenerative diseases... - Initiation of cancer and other diseases by catechol ortho-quinones: a unifying mechanismE L Cavalieri
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha 68198 6805, USA
Cell Mol Life Sci 59:665-81. 2002..These data suggest a unifying molecular mechanism of initiation for many cancers and neurodegenerative diseases and lay the groundwork for designing strategies to assess risk and prevent these diseases... - Depurinating estrogen-DNA adducts in the etiology and prevention of breast and other human cancersErcole L Cavalieri
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, NE 68198 6805, USA
Future Oncol 6:75-91. 2010..Two antioxidants, N-acetylcysteine and resveratrol, are prime candidates to prevent breast and other human cancers because in various M in vitro and in vivo experiments, they reduce the formation of estrogen-DNA adducts... - Identification and quantification of the depurinating DNA adducts formed in mouse skin treated with dibenzo[a,l]pyrene (DB[a,l]P) or its metabolites and in rat mammary gland treated with DB[a,l]PErcole L Cavalieri
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, NE 68198 6805, USA
Chem Res Toxicol 18:976-83. 2005..Only one depurinating diol epoxide adduct was formed. Tumorigenicity, mutations, and DNA adduct data suggest that depurinating Ade adducts play a major role in the initiation of tumors by DB[a,l]P... - Is bisphenol A a weak carcinogen like the natural estrogens and diethylstilbestrol?Ercole L Cavalieri
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, NE 68198 6805, USA
IUBMB Life 62:746-51. 2010..Knowledge of these mechanisms would allow us to begin to understand how BPA may act as a weak carcinogen and would be useful for regulating its use... - Imbalance of estrogen homeostasis in kidney and liver of hamsters treated with estradiol: implications for estrogen-induced initiation of renal tumorsE L Cavalieri
The Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, NE 68198 6805, USA
Chem Res Toxicol 14:1041-50. 2001..Thus, formation of depurinating DNA adducts by CE-3,4-Q may be the first critical event in the initiation of estrogen-induced kidney tumors... - Unbalanced metabolism of endogenous estrogens in the etiology and prevention of human cancerErcole L Cavalieri
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, NE 68198 6805, United States
J Steroid Biochem Mol Biol 125:169-80. 2011..Specific antioxidants, such as N-acetylcysteine and resveratrol, can block the oxidation of catechol estrogens to their quinones and their reaction with DNA. As a result, the initiation of cancer can be prevented... - A unified mechanism in the initiation of cancerErcole L Cavalieri
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, Nebraska 68198 6805, USA
Ann N Y Acad Sci 959:341-54. 2002..Thus, a unifying mechanism, namely formation of catechol quinones and reaction with DNA, could initiate not only cancer by oxidation of specific endogenous estrogen metabolites, but also leukemia by oxidation of benzene... - Catechol estrogen conjugates and DNA adducts in the kidney of male Syrian golden hamsters treated with 4-hydroxyestradiol: potential biomarkers for estrogen-initiated cancerP Devanesan
Eppley Institute for Research in Cancer and Allied Diseases, 986805 Nebraska Medical Center, Omaha, NE 68198-6805, USA
Carcinogenesis 22:489-97. 2001..We conclude that GSH and Cys conjugates of the 4-OHE and the 4-OHE-N7Gua adducts can be utilized as biomarkers to detect estrogenic imbalance and potential susceptibility to tumor initiation... - Metabolic activation and formation of DNA adducts of hexestrol, a synthetic nonsteroidal carcinogenic estrogenS T Jan
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 600 South 42nd Street, Omaha, Nebraska 68198 6805, USA
Chem Res Toxicol 11:412-9. 1998.... - Evidence that a burst of DNA depurination in SENCAR mouse skin induces error-prone repair and forms mutations in the H-ras geneD Chakravarti
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, Nebraska 68198 6805, USA
Oncogene 20:7945-53. 2001..These results also suggest that E(2)-3,4-Q, a suspected endogenous carcinogen, is a genotoxic compound and could cause mutations... - Evidence that error-prone DNA repair converts dibenzo[a,l]pyrene-induced depurinating lesions into mutations: formation, clonal proliferation and regression of initiated cells carrying H-ras oncogene mutations in early preneoplasiaD Chakravarti
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, NE 68198 6805, USA
Mutat Res 456:17-32. 2000..The multiplication of initiated stem cells during transient clonoplasia may be a factor determining the tumor-initiating potential of some PAH carcinogens... - Analysis of potential biomarkers of estrogen-initiated cancer in the urine of Syrian golden hamsters treated with 4-hydroxyestradiolR Todorovic
Eppley Institute for Research in Cancer and Allied Diseases, 986805 Nebraska Medical Center, Omaha, NE 68198-6805, USA
Carcinogenesis 22:905-11. 2001..The estrogen metabolites, conjugates and adducts can be used as biomarkers for detecting enzymatic oxidation of estrogens to reactive electrophilic metabolites and possible susceptibility to estrogen-induced cancer... - Benzene and dopamine catechol quinones could initiate cancer or neurogenic diseaseMuhammad Zahid
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, NE 68198 6805, USA
Free Radic Biol Med 48:318-24. 2010..Reaction of catechol quinones of natural and synthetic estrogens, benzene, naphthalene, and dopamine with DNA to form depurinating adducts is a common feature that may lead to initiation of cancer or neurodegenerative disease... - Profiles of cytokine mRNAs in the skin and lymph nodes of SENCAR mice treated epicutaneously with dibenzo[a,l]pyrene or dimethylbenz[a]anthracene reveal a direct correlation between carcinogen-induced contact hypersensitivity and epidermal hyperplasiaG P Casale
Eppley Institute for Research in Cancer, University of Nebraska Medical Center, Omaha 68198 6805, USA
Mol Carcinog 27:125-40. 2000..In conclusion, our data support carcinogen-specific CHS as a mechanism by which the very potent carcinogen DB[a,l]P induces epidermal hyperplasia, a requirement for tumor promotion in mouse skin... - Reduction of estrogen-induced transformation of mouse mammary epithelial cells by N-acetylcysteineDivya Venugopal
Eppley Institute for Research in Cancer and Allied Diseases, 986805 Nebraska Medical Center, Omaha, NE 68198 6805, United States
J Steroid Biochem Mol Biol 109:22-30. 2008..These results strongly implicate estrogen genotoxicity in mammary cell transformation. Since N-acetylcysteine is well tolerated in clinical studies, it may be a promising candidate for breast cancer prevention... - N-acetylcysteine blocks formation of cancer-initiating estrogen-DNA adducts in cellsMuhammad Zahid
Eppley Institute for Research in Cancer and Allied Diseases, College of Public Health, University of Nebraska Medical Center, Omaha, NE 68198, USA
Free Radic Biol Med 49:392-400. 2010..This finding suggests that NAcCys, a common dietary supplement, could be used as a potential chemopreventive agent to block the initial step in the genotoxicity caused by catechol estrogen quinones... - Role of human cytochrome P450 1A1, 1A2, 1B1, and 3A4 in the 2-, 4-, and 16alpha-hydroxylation of 17beta-estradiolA F Badawi
Eppley Institute for Research in Cancer, University of Nebraska Medical Center, Omaha, NE 68198 6805, USA
Metabolism 50:1001-3. 2001.... - Large-scale synthesis of the catechol metabolites of diethylstilbestrol and hexestrolS T Jan
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 600 South 42nd Street, Omaha, Nebraska 68198 6805, USA
Chem Res Toxicol 11:408-11. 1998..Using commercially inexpensive starting materials, this compound was synthesized in four steps, and the cis and trans isomers were separated and identified. Following the same synthetic route, 3'-OH-HES was synthesized in five steps... - Relative imbalances in the expression of estrogen-metabolizing enzymes in the breast tissue of women with breast carcinomaSeema Singh
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, NE 68198-6805, USA
Oncol Rep 14:1091-6. 2005..These unbalances appear to be instrumental in causing excessive formation of catechol estrogen quinones that, by reacting with DNA, initiate the series of events leading to breast cancer... - Evidence for NQO2-mediated reduction of the carcinogenic estrogen ortho-quinonesNilesh W Gaikwad
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, NE 68198 6805, USA
Free Radic Biol Med 46:253-62. 2009..Both UV and liquid chromatography-tandem mass spectrometry assays unequivocally corroborate the reduction of estrogen ortho-quinones by NQO2, indicating that it could be a novel target for prevention of breast cancer initiation... - Prevention of estrogen-DNA adduct formation in MCF-10F cells by resveratrolMuhammad Zahid
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, NE 68198 6805, USA
Free Radic Biol Med 45:136-45. 2008..We conclude that Resv can protect breast cells from carcinogenic estrogen metabolites, suggesting that it could be used in breast cancer prevention... - Urinary biomarkers suggest that estrogen-DNA adducts may play a role in the aetiology of non-Hodgkin lymphomaNilesh W Gaikwad
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, NE 68198 6805, USA
Biomarkers 14:502-12. 2009..Thus, formation of estrogen-DNA adducts may play a critical role in the aetiology of NHL, and these adducts could be potential biomarkers of NHL risk... - Metabolism and DNA binding studies of 4-hydroxyestradiol and estradiol-3,4-quinone in vitro and in female ACI rat mammary gland in vivoKai-Ming Li
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, NE 68198-6805, USA
Carcinogenesis 25:289-97. 2004..These adducts can generate the critical mutations that initiate cancer (Chakravarti et al., Oncogene, 2001, 20, 7945; Chakravarti et al., Proc. Am. Assoc. Cancer Res., 2003, 44, 180)... - Detection and quantification of depurinated benzo[a]pyrene-adducted DNA bases in the urine of cigarette smokers and women exposed to household coal smokeG P Casale
Eppley Institute for Research in Cancer, 986805, University of Nebraska Medical Center, Omaha, Nebraska 68198-6805, USA
Chem Res Toxicol 14:192-201. 2001..These results justify more extensive studies of depurinated BP-adducted DNA bases as potential biomarkers of PAH-associated cancer risk... - Relative imbalances in estrogen metabolism and conjugation in breast tissue of women with carcinoma: potential biomarkers of susceptibility to cancerEleanor G Rogan
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha 68198 6805, USA
Carcinogenesis 24:697-702. 2003..The levels of 4-CE and quinone conjugates were highly significant predictors of breast cancer. These results suggest that some catechol estrogen metabolites and conjugates could serve as biomarkers to predict risk of breast cancer... - Resveratrol prevents estrogen-DNA adduct formation and neoplastic transformation in MCF-10F cellsFang Lu
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, NE 68198 6805, USA
Cancer Prev Res (Phila) 1:135-45. 2008..Resveratrol also suppressed TCDD and/or estradiol-induced cell transformation. Thus, resveratrol can prevent breast cancer initiation by blocking multiple sites in the estrogen genotoxicity pathway... - Catechol estrogen metabolites and conjugates in different regions of the prostate of Noble rats treated with 4-hydroxyestradiol: implications for estrogen-induced initiation of prostate cancerErcole L Cavalieri
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, NE 68198 6805, USA
Carcinogenesis 23:329-33. 2002.... - Inhibition of depurinating estrogen-DNA adduct formation by natural compoundsMuhammad Zahid
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha 68198 6805, USA
Chem Res Toxicol 20:1947-53. 2007..This approach is highly useful for identifying agents to prevent the initiation of human cancers, especially breast and prostate cancer... - The role of polycyclic aromatic hydrocarbon-DNA adducts in inducing mutations in mouse skinDhrubajyoti Chakravarti
Eppley Institute for Research in Cancer and Allied Diseases, Omaha, NE 68198 6805, USA
Mutat Res 649:161-78. 2008..In conclusion, this study suggests that depurinating adducts play a major role in PAH mutagenesis... - Estrogen metabolites, conjugates, and DNA adducts: possible biomarkers for risk of breast, prostate, and other human cancersEleanor G Rogan
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA
Adv Clin Chem 38:135-49. 2004 - Estrogen metabolism and formation of estrogen-DNA adducts in estradiol-treated MCF-10F cells. The effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin induction and catechol-O-methyltransferase inhibitionFang Lu
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, NE 68198 6805, USA
J Steroid Biochem Mol Biol 105:150-8. 2007..We conclude that low COMT activity and increased formation of depurinating adducts can be critical factors leading to initiation of breast cancer... - Improved measurement of dibenzo[a,l]pyrene-induced abasic sites by the aldehyde-reactive probe assayDhrubajyoti Chakravarti
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, NE 68198 6805, USA
Mutat Res 588:158-65. 2005..In summary, the inclusion of ARP in the DB[a,l]P-DNA adduct-forming reaction traps and protects the nascent abasic sites, allowing an improved quantification of abasic sites... - Cytochrome P450 isoforms catalyze formation of catechol estrogen quinones that react with DNAYan Zhang
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, NE 68198 6805, USA
Metabolism 56:887-94. 2007.... - NAD(P)H:quinone oxidoreductase 1 Arg139Trp and Pro187Ser polymorphisms imbalance estrogen metabolism towards DNA adduct formation in human mammary epithelial cellsSeema Singh
Eppley Institute for Research in Cancer and Allied Diseases, 986805 Nebraska Medical Center, Omaha, NE 68198 6805, United States
J Steroid Biochem Mol Biol 117:56-66. 2009..Therefore, the inheritance of these NQO1 polymorphisms may favor the estrogen genotoxic mechanism of breast cancer... - Repair of short oligodeoxyribonucleotides containing a stable adduct and an apurinic site by extracts of MCF-10A1 cellsKimberly A Chapman
Eppley Institute for Research in Cancer and Allied Diseases and Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska 68198-6805, USA
Anticancer Res 24:117-21. 2004..CONCLUSION: The ability of human breast MCF-10A1 cells to repair DNA adducts and AP sites is affected by other near-by lesions... - The molecular etiology of breast cancer: evidence from biomarkers of riskNilesh W Gaikwad
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, NE 68198 6805, USA
Int J Cancer 122:1949-57. 2008..Thus, the depurinating estrogen-DNA adducts are possible biomarkers for early detection of breast cancer risk and response to preventive treatment... - Evidence from ESI-MS for NQO1-catalyzed reduction of estrogen ortho-quinonesNilesh W Gaikwad
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, NE 68198 6805, USA
Free Radic Biol Med 43:1289-98. 2007..Thus, clear evidence for the catalytic reduction of estrogen ortho-quinones by NQO1 has been obtained; its mechanism and implications are discussed... - Fluorobenzo[a]pyrenes as probes of the mechanism of cytochrome P450-catalyzed oxygen transfer in aromatic oxygenationsPatrick P J Mulder
Leiden Institute of Chemistry, Gorlaeus Laboratories, Leiden University, Leiden, The Netherlands
Free Radic Biol Med 34:734-45. 2003.... - Possible genotoxic modes of action for naphthaleneDavid Brusick
Department of Environmental Engineering, Carnegie Mellon University, USA
Regul Toxicol Pharmacol 51:S43-50. 2008..The report also outlines a set of six studies that could resolve some of these key uncertainties... - Mutagenic activity of 4-hydroxyestradiol, but not 2-hydroxyestradiol, in BB rat2 embryonic cells, and the mutational spectrum of 4-hydroxyestradiolZhonglin Zhao
New York University Dental School, New York, New York 10010, USA
Chem Res Toxicol 19:475-9. 2006..Thus, the results of this study support the proposal that estradiol can contribute to carcinogenesis via a genotoxic pathway... - Spectroscopic characterization of the 4-hydroxy catechol estrogen quinones-derived GSH and N-acetylated Cys conjugatesRyszard Jankowiak
Ames Laboratory, US Department of Energy and Department of Chemistry, Iowa State University, Ames, Iowa 50011, USA
Chem Res Toxicol 16:304-11. 2003..The concentration LOD is approximately 10(-9) M. Therefore, we propose that capillary electrophoresis interfaced with low temperature phosphorescence detection can be used to test for human exposure to CEQs by analyzing urine...
Research Grants
- MOLECULAR ORIGIN OF CANCER--CATECHOL/SEMIQUINOE/QUINONEErcole Cavalieri; Fiscal Year: 2003..In addition, we will identify biomarkers for use in diagnosis and in studies of prevention of breast cancer and other diseases. ..
- MECHANISMS OF CARCINOGENESIS OF DIBENZO(A,1)PYRENEErcole Cavalieri; Fiscal Year: 1993..The data obtained from the chemistry, tumorigenicity, DNA-binding studies and oncogene activation will provide significant clues concerning the mechanism of carcinogenesis of DB[a,l]P...
- Molecular Orgin Cancer--Catechol Estrogen 3 4 QuinonesErcole Cavalieri; Fiscal Year: 2004..The knowledge acquired from these studies will be essential for developing preventive strategies. ..