James Casanova

Summary

Affiliation: University of Virginia
Country: USA

Publications

  1. pmc Activation of ARF6 by ARNO stimulates epithelial cell migration through downstream activation of both Rac1 and phospholipase D
    L C Santy
    Department of Cell Biology, University of Virginia Health Sciences Center, Charlottesville, VA 22908, USA
    J Cell Biol 154:599-610. 2001
  2. pmc The guanine nucleotide exchange factor ARNO mediates the activation of ARF and phospholipase D by insulin
    Hai Sheng Li
    Department of Pharmacology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    BMC Cell Biol 4:13. 2003
  3. pmc PARtitioning numb
    James E Casanova
    Department of Cell Biology, University of Virginia Health System, Charlottesville, Virginia 22908 0732, USA
    EMBO Rep 8:233-5. 2007
  4. ncbi request reprint Epithelial cell cytoskeleton and intracellular trafficking V. Confluence of membrane trafficking and motility in epithelial cell models
    James E Casanova
    Department of Cell Biology, University of Virginia Health System, Charlottesville, Virginia 22908 0732, USA
    Am J Physiol Gastrointest Liver Physiol 283:G1015-9. 2002
  5. ncbi request reprint ARFs
    James E Casanova
    Department of Cell Biology, University of Virginia Health System, 1300 Jefferson Park Ave, Charlottesville, VA 22908 0732, USA
    Curr Biol 13:R123. 2003
  6. ncbi request reprint Regulation of Arf activation: the Sec7 family of guanine nucleotide exchange factors
    James E Casanova
    Department of Cell Biology, University of Virginia Health System, Charlottesville, VA 22908 0732, USA
    Traffic 8:1476-85. 2007
  7. pmc SCAMP2 interacts with Arf6 and phospholipase D1 and links their function to exocytotic fusion pore formation in PC12 cells
    Lixia Liu
    Department of Cell Biology, University of Virginia Health System, School of Medicine, Charlottesville, VA 22908, USA
    Mol Biol Cell 16:4463-72. 2005
  8. ncbi request reprint Nuclear functions of the Arf guanine nucleotide exchange factor BRAG2
    Jillian L Dunphy
    Department of Cell Biology, University of Virginia Health Sciences Center, Box 800732, Charlottesville, VA 22908, USA
    Traffic 8:661-72. 2007
  9. ncbi request reprint WAVE2 signaling mediates invasion of polarized epithelial cells by Salmonella typhimurium
    Jing Shi
    Department of Cell Biology, University of Virginia Health Sciences Center, Charlottesville, 22908 0732, USA
    J Biol Chem 280:29849-55. 2005
  10. pmc The Arf6 GEF GEP100/BRAG2 regulates cell adhesion by controlling endocytosis of beta1 integrins
    Jillian L Dunphy
    Department of Cell Biology, University of Virginia Health Sciences Center, Box 800732, Charlottesville, Virginia 22908, USA
    Curr Biol 16:315-20. 2006

Collaborators

Detail Information

Publications31

  1. pmc Activation of ARF6 by ARNO stimulates epithelial cell migration through downstream activation of both Rac1 and phospholipase D
    L C Santy
    Department of Cell Biology, University of Virginia Health Sciences Center, Charlottesville, VA 22908, USA
    J Cell Biol 154:599-610. 2001
    ....
  2. pmc The guanine nucleotide exchange factor ARNO mediates the activation of ARF and phospholipase D by insulin
    Hai Sheng Li
    Department of Pharmacology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    BMC Cell Biol 4:13. 2003
    ..Here we tested the hypothesis that ARF-guanine nucleotide exchange factors (ARF-GEFs) of the cytohesin/ARNO family mediate the activation of ARF and PLD by insulin...
  3. pmc PARtitioning numb
    James E Casanova
    Department of Cell Biology, University of Virginia Health System, Charlottesville, Virginia 22908 0732, USA
    EMBO Rep 8:233-5. 2007
  4. ncbi request reprint Epithelial cell cytoskeleton and intracellular trafficking V. Confluence of membrane trafficking and motility in epithelial cell models
    James E Casanova
    Department of Cell Biology, University of Virginia Health System, Charlottesville, Virginia 22908 0732, USA
    Am J Physiol Gastrointest Liver Physiol 283:G1015-9. 2002
    ..In this themes article, I review current hypotheses about how these processes are integrated and attempt to identify fruitful areas for future research...
  5. ncbi request reprint ARFs
    James E Casanova
    Department of Cell Biology, University of Virginia Health System, 1300 Jefferson Park Ave, Charlottesville, VA 22908 0732, USA
    Curr Biol 13:R123. 2003
  6. ncbi request reprint Regulation of Arf activation: the Sec7 family of guanine nucleotide exchange factors
    James E Casanova
    Department of Cell Biology, University of Virginia Health System, Charlottesville, VA 22908 0732, USA
    Traffic 8:1476-85. 2007
    ..Here we review recent progress in our understanding of the structure, localization and biology of the different classes of Arf GEFs...
  7. pmc SCAMP2 interacts with Arf6 and phospholipase D1 and links their function to exocytotic fusion pore formation in PC12 cells
    Lixia Liu
    Department of Cell Biology, University of Virginia Health System, School of Medicine, Charlottesville, VA 22908, USA
    Mol Biol Cell 16:4463-72. 2005
    ..However, mutant SCAMP2 also uniquely inhibits fusion pore dilation. Thus, SCAMP2 couples Arf6-stimulated PLD activity to exocytosis and links this process to formation of fusion pores...
  8. ncbi request reprint Nuclear functions of the Arf guanine nucleotide exchange factor BRAG2
    Jillian L Dunphy
    Department of Cell Biology, University of Virginia Health Sciences Center, Box 800732, Charlottesville, VA 22908, USA
    Traffic 8:661-72. 2007
    ..These observations suggest that, in addition to its role in endocytic regulation at the plasma membrane, BRAG2 also functions within the nucleus...
  9. ncbi request reprint WAVE2 signaling mediates invasion of polarized epithelial cells by Salmonella typhimurium
    Jing Shi
    Department of Cell Biology, University of Virginia Health Sciences Center, Charlottesville, 22908 0732, USA
    J Biol Chem 280:29849-55. 2005
    ..Although infection leads to the formation of an IRSp53/WAVE2 complex, it is the association of WAVE2 with the Abi1/Nap1/PIR121/HSPC300 complex that regulates bacterial internalization...
  10. pmc The Arf6 GEF GEP100/BRAG2 regulates cell adhesion by controlling endocytosis of beta1 integrins
    Jillian L Dunphy
    Department of Cell Biology, University of Virginia Health Sciences Center, Box 800732, Charlottesville, Virginia 22908, USA
    Curr Biol 16:315-20. 2006
    ..These findings suggest that Arf6 regulates both endocytosis and recycling of beta1 integrins and that BRAG2 functions selectively to activate Arf6 during integrin internalization...
  11. pmc Regulation of Arf6 and ACAP1 signaling by the PTB-domain-containing adaptor protein GULP
    Zhong Ma
    Carter Immunology Center, Department of Microbiology, University of Virginia, Charlottesville, Virginia 22908, USA
    Curr Biol 17:722-7. 2007
    ..Because PTB-domain-containing adaptor proteins influence endocytosis and trafficking of membrane proteins and cell migration [15, 16], our data support a model wherein PTB-domain-containing adaptor proteins regulate Arf family proteins...
  12. pmc Coordinate regulation of Salmonella enterica serovar Typhimurium invasion of epithelial cells by the Arp2/3 complex and Rho GTPases
    Alison K Criss
    Department of Cell Biology, University of Virginia Health Sciences Center, Charlottesville, Virginia 22908 0732, USA
    Infect Immun 71:2885-91. 2003
    ..enterica serovar Typhimurium SopE are individually required for basolateral internalization. These results underscore that the specific factors required for Salmonella invasion differ between membrane domains of polarized epithelia...
  13. ncbi request reprint The DOCK180/Elmo complex couples ARNO-mediated Arf6 activation to the downstream activation of Rac1
    Lorraine C Santy
    Department of Cell Biology, University of Virginia, Charlottesville, Virginia 22908, USA
    Curr Biol 15:1749-54. 2005
    ..Together, these data suggest that ARNO and ARF6 coordinate with the Dock180/Elmo complex to promote Rac activation at the leading edge of migrating cells...
  14. pmc Regulation of actin cytoskeleton dynamics by Arf-family GTPases
    Kenneth R Myers
    Department of Cell Biology, University of Virginia Health System, Charlottesville, VA 22908 0732, USA
    Trends Cell Biol 18:184-92. 2008
    ..Here, we review recent progress in our understanding of how Arfs and their interacting proteins function to integrate membrane and cytoskeletal dynamics...
  15. pmc A fluorescence resonance energy transfer activation sensor for Arf6
    Brian Hall
    Robert M Berne Cardiovascular Research Center, University of Virginia, Charlottesville, VA 22908, USA
    Anal Biochem 374:243-9. 2008
    ..The addition of platelet-derived growth factor (PDGF) to fibroblasts triggered a rapid and transient increase in FRET, indicative of Arf6 activation. These reagents should be useful for investigations of Arf6 activation and function...
  16. ncbi request reprint Substrate specificities and activities of AZAP family Arf GAPs in vivo
    Ellen J Cuthbert
    Department of Cell Biology, University of Virginia Health System, Charlottesville, VA 22908 0732, USA
    Am J Physiol Cell Physiol 294:C263-70. 2008
    ..Finally, we found that expression of AGAP1 induces a surprising reciprocal activation of Arf6, which suggests that regulatory cross talk exists among Arf isoforms...
  17. ncbi request reprint GTPase signaling: bridging the GAP between ARF and Rho
    Lorraine C Santy
    Department of Cell Biology, University of Virginia, Health Sciences Center, P O Box 800732, Charlottesville, Virginia 22908 0732, USA
    Curr Biol 12:R360-2. 2002
    ..Recent studies have identified a family of GTPase activating proteins (GAPs) that contain both ARF-GAP and Rho-GAP domains, providing the first direct link between these two signaling pathways...
  18. pmc Invasion of host cells by Salmonella typhimurium requires focal adhesion kinase and p130Cas
    Jing Shi
    Department of Cell Biology, University of Virginia Health Sciences Center, Charlottesville, VA 22908 0732, USA
    Mol Biol Cell 17:4698-708. 2006
    ..Together, these findings reveal a novel role for focal adhesion proteins in the invasion of host cells by Salmonella...
  19. ncbi request reprint Mechanisms of Salmonella entry into host cells
    Kim Thien Ly
    Department of Microbiology, University of Virginia Health System, Charlottesville, VA 22908 0732, USA
    Cell Microbiol 9:2103-11. 2007
    ..This review is intended to summarize our current understanding of the tools used by Salmonella to enter host cells, with a focus on effectors that modulate the actin cytoskeleton...
  20. pmc Abelson tyrosine kinase facilitates Salmonella enterica serovar Typhimurium entry into epithelial cells
    Kim Thien Ly
    Department of Microbiology, University of Virginia Health System, Charlottesville, Virginia 22908 0732, USA
    Infect Immun 77:60-9. 2009
    ..Taken together, these findings indicate that c-Abl is activated during S. enterica serovar Typhimurium infection and that its phosphorylation of multiple downstream targets is functionally important in bacterial internalization...
  21. pmc Arf6 and microtubules in adhesion-dependent trafficking of lipid rafts
    Nagaraj Balasubramanian
    Robert M Beirne Cardiovascular Research Center, Mellon Prostate Cancer Research Institute, University of Virginia, Charlottesville, Virginia 22908, USA
    Nat Cell Biol 9:1381-91. 2007
    ..Both of these steps are required for the plasma membrane targeting of Rac1 and for its activation. These data therefore define a new membrane raft trafficking pathway that is crucial for anchorage-dependent signalling...
  22. ncbi request reprint ARAP2 effects on the actin cytoskeleton are dependent on Arf6-specific GTPase-activating-protein activity and binding to RhoA-GTP
    Hye Young Yoon
    Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, Department of Health and Human Services, Building 37, Bethesda, MD 20892, USA
    J Cell Sci 119:4650-66. 2006
    ..We conclude that ARAP2 is an Arf6GAP that functions downstream of RhoA to regulate focal adhesion dynamics...
  23. ncbi request reprint ARNO through its coiled-coil domain regulates endocytosis at the apical surface of polarized epithelial cells
    Miriam Shmuel
    Department of Pharmacology, School of Pharmacy, Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem 91120, Israel
    J Biol Chem 281:13300-8. 2006
    ..We conclude that ARNO acts together with ARF6 to regulate apical endocytosis...
  24. ncbi request reprint Interaction of the Salmonella-containing vacuole with the endocytic recycling system
    Adam C Smith
    Infection, Immunity, Injury, and Repair Program, Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada
    J Biol Chem 280:24634-41. 2005
    ..We also demonstrate the utility of using Salmonella invasion as a model of endosomal recycling events...
  25. pmc The TBC (Tre-2/Bub2/Cdc16) domain protein TRE17 regulates plasma membrane-endosomal trafficking through activation of Arf6
    Lenka Martinu
    University of Pennsylvania School of Medicine, Department of Cell and Developmental Biology, 421 Curie Blvd, BRBII Room 1011, Philadelphia, PA 19104 6160, USA
    Mol Cell Biol 24:9752-62. 2004
    ..These studies identify TRE17 as a novel regulator of the Arf6-regulated plasma membrane recycling system and reveal an unexpected function for TBC domains...
  26. ncbi request reprint Salmonella typhimurium transcytoses flagellin via an SPI2-mediated vesicular transport pathway
    Sean Lyons
    Epithelial Pathobiology Division, Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA 30322, USA
    J Cell Sci 117:5771-80. 2004
    ....
  27. pmc ARNO and ARF6 regulate axonal elongation and branching through downstream activation of phosphatidylinositol 4-phosphate 5-kinase alpha
    Delia J Hernandez-Deviez
    Department of Cell Biology and Anatomy, College of Medicine, University of Arizona, Tucson, Arizona 85724, USA
    Mol Biol Cell 15:111-20. 2004
    ..These results suggest that ARNO and ARF6, through PI(4)P 5-Kinase alpha, regulate axonal elongation and branching during neuronal development...
  28. pmc Rab14 regulates apical targeting in polarized epithelial cells
    Khameeka N Kitt
    Department of Cell Biology and Anatomy, Arizona Health Sciences Center, University of Arizona, PO Box 245044, Tucson, AZ 85724, USA
    Traffic 9:1218-31. 2008
    ..These data suggest that Rab14 specifically regulates delivery of cargo from the TGN to the apical domain...
  29. ncbi request reprint ARF6: a newly appreciated player in G protein-coupled receptor desensitization
    Mary Hunzicker-Dunn
    Department of Cell and Molecular Biology, Northwestern University Medical School, 303 East Chicago Avenue, Chicago, IL 60611, USA
    FEBS Lett 521:3-8. 2002
    ..We hypothesize that ARF6 might also serve GPCRs other than the LH/CG R to regulate the availability of arrestin 2 for receptor desensitization...
  30. ncbi request reprint Regulation of dendritic development by the ARF exchange factor ARNO
    Delia J Hernandez-Deviez
    Department of Cell Biology and Anatomy, College of Medicine, University of Arizona, Box 245044, Tucson, Arizona 85718, USA
    Nat Neurosci 5:623-4. 2002
    ....

Research Grants23

  1. TRANSEPITHELIAL TRANSPORT OF IMMUNOGLOBULINS
    James Casanova; Fiscal Year: 2000
    ..These studies will provide insight into the mechanisms by which macromolecules are transported across epithelia, a process that is particularly important in the mucosal immunity. ..
  2. MECHANISMS OF SALMONELLA INVASION AND TRANSMIGRATION
    James Casanova; Fiscal Year: 2007
    ..These hypotheses will be tested in specifc Aim 3. The overall goal of the proposed research is to determine the mechanisms by which Salmonella enter host intestinal cells and cause disease. ..
  3. The Role of Arf GTPases in Endocytosis and Postendocytic Transport
    James Casanova; Fiscal Year: 2007
    ....
  4. MECHANISMS OF SALMONELLA INVASION AND TRANSMIGRATION
    James Casanova; Fiscal Year: 2006
    ..These hypotheses will be tested in specifc Aim 3. The overall goal of the proposed research is to determine the mechanisms by which Salmonella enter host intestinal cells and cause disease. ..
  5. The Role of Arf GTPases in Endocytosis and Postendocytic Transport
    James Casanova; Fiscal Year: 2009
    ....
  6. MECHANISMS OF SALMONELLA INVASION AND TRANSMIGRATION
    James Casanova; Fiscal Year: 2009
    ..These hypotheses will be tested in specifc Aim 3. The overall goal of the proposed research is to determine the mechanisms by which Salmonella enter host intestinal cells and cause disease. ..
  7. The Role of Arf GTPases in Endocytosis and Postendocytic Transport
    James E Casanova; Fiscal Year: 2010
    ....
  8. The Role of Arf GTPases in Endocytosis and Postendocytic Transport
    James Casanova; Fiscal Year: 2009
    ....
  9. MECHANISMS OF SALMONELLA INVASION AND TRANSMIGRATION
    James Casanova; Fiscal Year: 2006
    ..These hypotheses will be tested in specifc Aim 3. The overall goal of the proposed research is to determine the mechanisms by which Salmonella enter host intestinal cells and cause disease. ..
  10. MECHANISMS OF SALMONELLA INVASION AND TRANSMIGRATION
    James Casanova; Fiscal Year: 2005
    ..These hypotheses will be tested in specifc Aim 3. The overall goal of the proposed research is to determine the mechanisms by which Salmonella enter host intestinal cells and cause disease. ..
  11. Role of the GTPase ARF6 in Epithelial Cell Migration
    James Casanova; Fiscal Year: 2005
    ..abstract_text> ..
  12. TRANSEPITHELIAL TRANSPORT OF IMMUNOGLOBULINS
    James Casanova; Fiscal Year: 1999
    ..These studies will provide insight into the mechanisms by which macromolecules are transported across epithelia, a process that is particularly important in the mucosal immunity. ..
  13. MECHANISMS OF SALMONELLA INVASION AND TRANSMIGRATION
    James Casanova; Fiscal Year: 2001
    ..The results of these studies will provide significant insight into the mechanisms of Salmonella pathogenesis within the intestinal epithelium. ..
  14. TRANSEPITHELIAL TRANSPORT OF IMMUNOGLOBULINS
    James Casanova; Fiscal Year: 2001
    ..These studies will provide insight into the mechanisms by which macromolecules are transported across epithelia, a process that is particularly important in the mucosal immunity. ..
  15. TRANSEPITHELIAL TRANSPORT OF IMMUNOGLOBULINS
    James Casanova; Fiscal Year: 2002
    ..These studies will provide insight into the mechanisms by which macromolecules are transported across epithelia, a process that is particularly important in the mucosal immunity. ..
  16. MECHANISMS OF SALMONELLA INVASION AND TRANSMIGRATION
    James Casanova; Fiscal Year: 2002
    ..The results of these studies will provide significant insight into the mechanisms of Salmonella pathogenesis within the intestinal epithelium. ..
  17. Role of the GTPase ARF6 in Epithelial Cell Migration
    James Casanova; Fiscal Year: 2002
    ..abstract_text> ..
  18. MECHANISMS OF SALMONELLA INVASION AND TRANSMIGRATION
    James Casanova; Fiscal Year: 2003
    ..The results of these studies will provide significant insight into the mechanisms of Salmonella pathogenesis within the intestinal epithelium. ..
  19. Role of the GTPase ARF6 in Epithelial Cell Migration
    James Casanova; Fiscal Year: 2003
    ..abstract_text> ..
  20. Role of the GTPase ARF6 in Epithelial Cell Migration
    James Casanova; Fiscal Year: 2004
    ..abstract_text> ..
  21. MECHANISMS OF SALMONELLA INVASION AND TRANSMIGRATION
    James E Casanova; Fiscal Year: 2010
    ..The goals of this research are to define the host cell machinery that is subverted by Salmonella to enter the intestinal epithelium and spread systemically, and to characterize the innate immune responses to Salmonella infection. ..