Dana Carroll

Summary

Affiliation: University of Utah
Country: USA

Publications

  1. ncbi request reprint Zinc-finger nucleases: a panoramic view
    Dana Carroll
    Department of Biochemistry, University of Utah School of Medicine, 15 N Medical Drive East, Salt Lake City, UT 84112 5650, USA
    Curr Gene Ther 11:2-10. 2011
  2. ncbi request reprint Design, construction and in vitro testing of zinc finger nucleases
    Dana Carroll
    Department of Biochemistry, University of Utah School of Medicine, 15 N Medical Drive East, Room 4100, Salt Lake City, Utah 84112, USA
    Nat Protoc 1:1329-41. 2006
  3. pmc Genome engineering with zinc-finger nucleases
    Dana Carroll
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah 84112 5650, USA
    Genetics 188:773-82. 2011
  4. pmc Progress and prospects: zinc-finger nucleases as gene therapy agents
    D Carroll
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT 84112 5650, USA
    Gene Ther 15:1463-8. 2008
  5. pmc Efficient gene targeting in Drosophila by direct embryo injection with zinc-finger nucleases
    Kelly J Beumer
    Department of Biochemistry, University of Utah School of Medicine, 15 North Medical Drive East, Salt Lake City, UT 84112 5650, USA
    Proc Natl Acad Sci U S A 105:19821-6. 2008
  6. pmc Genetic analysis of zinc-finger nuclease-induced gene targeting in Drosophila
    Ana Bozas
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah 84112 5650, USA
    Genetics 182:641-51. 2009
  7. doi request reprint Gene targeting in Drosophila and Caenorhabditis elegans with zinc-finger nucleases
    Dana Carroll
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT, USA
    Methods Mol Biol 435:63-77. 2008
  8. pmc Efficient gene targeting in Drosophila with zinc-finger nucleases
    Kelly Beumer
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah 84112, USA
    Genetics 172:2391-403. 2006
  9. ncbi request reprint Enhancing gene targeting with designed zinc finger nucleases
    Marina Bibikova
    Department of Biochemistry, University of Utah School of Medicine, 20 North 1900 East, Salt Lake City, UT 84132 3201, USA
    Science 300:764. 2003
  10. doi request reprint High-efficiency gene targeting in Drosophila with zinc finger nucleases
    Dana Carroll
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT, USA
    Methods Mol Biol 649:271-80. 2010

Research Grants

  1. Gene Targeting in Flies with ZFNs
    Dana Carroll; Fiscal Year: 2010
  2. Gene Targeting in Flies and Worms with ZFNs
    Dana Carroll; Fiscal Year: 2009
  3. Stimulating Gene Targeting with Zinc Finger Nucleases
    Dana Carroll; Fiscal Year: 2004
  4. Chimeric Nucleases for Gene Targeting in Drosophila
    Dana Carroll; Fiscal Year: 2003
  5. CHIMERIC NUCLEASES FOR GENE TARGETING
    Dana Carroll; Fiscal Year: 2000
  6. Gene Targeting in Flies and Worms with ZFNs
    Dana Carroll; Fiscal Year: 2009

Collaborators

Detail Information

Publications15

  1. ncbi request reprint Zinc-finger nucleases: a panoramic view
    Dana Carroll
    Department of Biochemistry, University of Utah School of Medicine, 15 N Medical Drive East, Salt Lake City, UT 84112 5650, USA
    Curr Gene Ther 11:2-10. 2011
    ..This article reviews discoveries that led to the development of ZFNs, cites examples of successes in genome engineering, and projects how ZFNs may be used in the future, particularly in applications to humans...
  2. ncbi request reprint Design, construction and in vitro testing of zinc finger nucleases
    Dana Carroll
    Department of Biochemistry, University of Utah School of Medicine, 15 N Medical Drive East, Room 4100, Salt Lake City, Utah 84112, USA
    Nat Protoc 1:1329-41. 2006
    ..If all goes smoothly, design, construction and cloning can be completed in about two weeks, with expression and testing completed in one additional week...
  3. pmc Genome engineering with zinc-finger nucleases
    Dana Carroll
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah 84112 5650, USA
    Genetics 188:773-82. 2011
    ..The bright prospects for future applications of ZFNs, including human gene therapy, are discussed...
  4. pmc Progress and prospects: zinc-finger nucleases as gene therapy agents
    D Carroll
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT 84112 5650, USA
    Gene Ther 15:1463-8. 2008
    ..New tools are available for the design and testing of ZFNs for new target genes. Applications of ZFNs to stem cells have been described, and genuine gene therapy trials appear to be on the immediate horizon...
  5. pmc Efficient gene targeting in Drosophila by direct embryo injection with zinc-finger nucleases
    Kelly J Beumer
    Department of Biochemistry, University of Utah School of Medicine, 15 North Medical Drive East, Salt Lake City, UT 84112 5650, USA
    Proc Natl Acad Sci U S A 105:19821-6. 2008
    ..The injection procedure is much more rapid than earlier approaches and makes possible the generation and recovery of targeted gene alterations at essentially any locus within 2 fly generations...
  6. pmc Genetic analysis of zinc-finger nuclease-induced gene targeting in Drosophila
    Ana Bozas
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah 84112 5650, USA
    Genetics 182:641-51. 2009
    ..They also demonstrate that the outcome can be biased toward gene replacement by disabling the major NHEJ pathway and toward simple mutagenesis by interfering with the major HR process...
  7. doi request reprint Gene targeting in Drosophila and Caenorhabditis elegans with zinc-finger nucleases
    Dana Carroll
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT, USA
    Methods Mol Biol 435:63-77. 2008
    ..We describe the construction of ZFNs for novel targets and their application to targeted mutagenesis and targeted gene replacement in Drosophila melanogaster and Caenorhabditis elegans...
  8. pmc Efficient gene targeting in Drosophila with zinc-finger nucleases
    Kelly Beumer
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah 84112, USA
    Genetics 172:2391-403. 2006
    ....
  9. ncbi request reprint Enhancing gene targeting with designed zinc finger nucleases
    Marina Bibikova
    Department of Biochemistry, University of Utah School of Medicine, 20 North 1900 East, Salt Lake City, UT 84132 3201, USA
    Science 300:764. 2003
  10. doi request reprint High-efficiency gene targeting in Drosophila with zinc finger nucleases
    Dana Carroll
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT, USA
    Methods Mol Biol 649:271-80. 2010
    ..A donor DNA carrying desired sequence changes can be incorporated in the injection mix and leads to targeted gene replacement, with particularly good efficiency when the recipient embryos are defective for nonhomologous end joining...
  11. ncbi request reprint Using nucleases to stimulate homologous recombination
    Dana Carroll
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, USA
    Methods Mol Biol 262:195-207. 2004
    ..Zinc-finger nucleases have the potential of being targetable to arbitrarily selected sites, owing to the flexibility of zinc finger recognition of DNA...
  12. pmc Targeted mutagenesis using zinc-finger nucleases in Arabidopsis
    Alan Lloyd
    Department of Biology, University of Utah, 257 South 1400 East, Salt Lake City, UT 84112 0840, USA
    Proc Natl Acad Sci U S A 102:2232-7. 2005
    ..These data indicate that ZFNs can form the basis of a highly efficient method for targeted mutagenesis of plant genes...
  13. pmc Induction and repair of zinc-finger nuclease-targeted double-strand breaks in Caenorhabditis elegans somatic cells
    Jason Morton
    Department of Biochemistry, University of Utah School of Medicine, 15 North Medical Drive East, Salt Lake City, UT 84112, USA
    Proc Natl Acad Sci U S A 103:16370-5. 2006
    ..DNA ligase IV is required for efficient end joining, particularly of blunt ends. In its absence, a secondary end-joining pathway relies more heavily on microhomologies in producing deletions...
  14. pmc Targeted chromosomal cleavage and mutagenesis in Drosophila using zinc-finger nucleases
    Marina Bibikova
    Department of Biochemistry, University of Utah School of Medicine, Medical Research and Education Building, 20 North 1900 East, Salt Lake City, UT 84132, USA
    Genetics 161:1169-75. 2002
    ..This approach promises to permit generation of directed mutations in many types of cells and organisms...
  15. ncbi request reprint Gene targeting using zinc finger nucleases
    Matthew H Porteus
    Department of Pediatrics, University of Texas Southwestern Medical Center, USA
    Nat Biotechnol 23:967-73. 2005
    ..Future work will be needed to translate these in vitro findings to in vivo applications and to determine whether zinc finger nucleases create undesired genomic instability...

Research Grants14

  1. Gene Targeting in Flies with ZFNs
    Dana Carroll; Fiscal Year: 2010
    ..They will provide information on the best methods for creating models of human disease in experimental organisms and for modifying genes in applications to human gene therapy. ..
  2. Gene Targeting in Flies and Worms with ZFNs
    Dana Carroll; Fiscal Year: 2009
    ..Gene targeting can be used to create animal models for human disease and, ultimately, for human gene therapy. ..
  3. Stimulating Gene Targeting with Zinc Finger Nucleases
    Dana Carroll; Fiscal Year: 2004
    ..To demonstrate the generality of this approach to gene targeting, it will be applied to another mouse locus, the Rpo2-1 gene. ..
  4. Chimeric Nucleases for Gene Targeting in Drosophila
    Dana Carroll; Fiscal Year: 2003
    ..To test the generality of the method, it will be applied to a second Drosophila locus, the rosy (ry) gene. ..
  5. CHIMERIC NUCLEASES FOR GENE TARGETING
    Dana Carroll; Fiscal Year: 2000
    ..This will prepare the way for targeting of specific sites in complex genomes. ..
  6. Gene Targeting in Flies and Worms with ZFNs
    Dana Carroll; Fiscal Year: 2009
    ..Gene targeting can be used to create animal models for human disease and, ultimately, for human gene therapy. ..