Dana Carroll

Summary

Affiliation: University of Utah
Country: USA

Publications

  1. ncbi request reprint Zinc-finger nucleases: a panoramic view
    Dana Carroll
    Department of Biochemistry, University of Utah School of Medicine, 15 N Medical Drive East, Salt Lake City, UT 84112 5650, USA
    Curr Gene Ther 11:2-10. 2011
  2. ncbi request reprint Design, construction and in vitro testing of zinc finger nucleases
    Dana Carroll
    Department of Biochemistry, University of Utah School of Medicine, 15 N Medical Drive East, Room 4100, Salt Lake City, Utah 84112, USA
    Nat Protoc 1:1329-41. 2006
  3. ncbi request reprint Genome editing by targeted chromosomal mutagenesis
    Dana Carroll
    Department of Biochemistry, School of Medicine, University of Utah, Emma Eccles Jones Medical Research Building, Rm 4520, 15N Medical Drive East, Salt Lake City, UT, 84112 5650, USA
    Methods Mol Biol 1239:1-13. 2015
  4. ncbi request reprint Genome engineering with TALENs and ZFNs: repair pathways and donor design
    Dana Carroll
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT 84112 5650, USA Electronic address
    Methods 69:137-41. 2014
  5. ncbi request reprint Genome engineering with targetable nucleases
    Dana Carroll
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah 84112 email
    Annu Rev Biochem 83:409-39. 2014
  6. pmc Genome engineering with zinc-finger nucleases
    Dana Carroll
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah 84112 5650, USA
    Genetics 188:773-82. 2011
  7. pmc Progress and prospects: zinc-finger nucleases as gene therapy agents
    D Carroll
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT 84112 5650, USA
    Gene Ther 15:1463-8. 2008
  8. ncbi request reprint Gene targeting in Drosophila and Caenorhabditis elegans with zinc-finger nucleases
    Dana Carroll
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT, USA
    Methods Mol Biol 435:63-77. 2008
  9. pmc Genetic analysis of zinc-finger nuclease-induced gene targeting in Drosophila
    Ana Bozas
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah 84112 5650, USA
    Genetics 182:641-51. 2009
  10. pmc Efficient gene targeting in Drosophila by direct embryo injection with zinc-finger nucleases
    Kelly J Beumer
    Department of Biochemistry, University of Utah School of Medicine, 15 North Medical Drive East, Salt Lake City, UT 84112 5650, USA
    Proc Natl Acad Sci U S A 105:19821-6. 2008

Research Grants

Collaborators

Detail Information

Publications22

  1. ncbi request reprint Zinc-finger nucleases: a panoramic view
    Dana Carroll
    Department of Biochemistry, University of Utah School of Medicine, 15 N Medical Drive East, Salt Lake City, UT 84112 5650, USA
    Curr Gene Ther 11:2-10. 2011
    ..This article reviews discoveries that led to the development of ZFNs, cites examples of successes in genome engineering, and projects how ZFNs may be used in the future, particularly in applications to humans...
  2. ncbi request reprint Design, construction and in vitro testing of zinc finger nucleases
    Dana Carroll
    Department of Biochemistry, University of Utah School of Medicine, 15 N Medical Drive East, Room 4100, Salt Lake City, Utah 84112, USA
    Nat Protoc 1:1329-41. 2006
    ..If all goes smoothly, design, construction and cloning can be completed in about two weeks, with expression and testing completed in one additional week...
  3. ncbi request reprint Genome editing by targeted chromosomal mutagenesis
    Dana Carroll
    Department of Biochemistry, School of Medicine, University of Utah, Emma Eccles Jones Medical Research Building, Rm 4520, 15N Medical Drive East, Salt Lake City, UT, 84112 5650, USA
    Methods Mol Biol 1239:1-13. 2015
    ..These reagents are being used to introduce favorable characteristics into organisms of economic significance, and the prospects for enhancing human gene therapy appear very bright. ..
  4. ncbi request reprint Genome engineering with TALENs and ZFNs: repair pathways and donor design
    Dana Carroll
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT 84112 5650, USA Electronic address
    Methods 69:137-41. 2014
    ..This article reviews the influence of repair mechanisms on the results of gene targeting experiments, with an emphasis on lessons learned from experiments with Drosophila...
  5. ncbi request reprint Genome engineering with targetable nucleases
    Dana Carroll
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah 84112 email
    Annu Rev Biochem 83:409-39. 2014
    ..This review discusses the properties, advantages, and limitations of each system, as well as the specific considerations required for their use in different biological systems. ..
  6. pmc Genome engineering with zinc-finger nucleases
    Dana Carroll
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah 84112 5650, USA
    Genetics 188:773-82. 2011
    ..The bright prospects for future applications of ZFNs, including human gene therapy, are discussed...
  7. pmc Progress and prospects: zinc-finger nucleases as gene therapy agents
    D Carroll
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT 84112 5650, USA
    Gene Ther 15:1463-8. 2008
    ..New tools are available for the design and testing of ZFNs for new target genes. Applications of ZFNs to stem cells have been described, and genuine gene therapy trials appear to be on the immediate horizon...
  8. ncbi request reprint Gene targeting in Drosophila and Caenorhabditis elegans with zinc-finger nucleases
    Dana Carroll
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT, USA
    Methods Mol Biol 435:63-77. 2008
    ..We describe the construction of ZFNs for novel targets and their application to targeted mutagenesis and targeted gene replacement in Drosophila melanogaster and Caenorhabditis elegans...
  9. pmc Genetic analysis of zinc-finger nuclease-induced gene targeting in Drosophila
    Ana Bozas
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah 84112 5650, USA
    Genetics 182:641-51. 2009
    ..They also demonstrate that the outcome can be biased toward gene replacement by disabling the major NHEJ pathway and toward simple mutagenesis by interfering with the major HR process...
  10. pmc Efficient gene targeting in Drosophila by direct embryo injection with zinc-finger nucleases
    Kelly J Beumer
    Department of Biochemistry, University of Utah School of Medicine, 15 North Medical Drive East, Salt Lake City, UT 84112 5650, USA
    Proc Natl Acad Sci U S A 105:19821-6. 2008
    ..The injection procedure is much more rapid than earlier approaches and makes possible the generation and recovery of targeted gene alterations at essentially any locus within 2 fly generations...
  11. pmc Efficient gene targeting in Drosophila with zinc-finger nucleases
    Kelly Beumer
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah 84112, USA
    Genetics 172:2391-403. 2006
    ....
  12. ncbi request reprint Enhancing gene targeting with designed zinc finger nucleases
    Marina Bibikova
    Department of Biochemistry, University of Utah School of Medicine, 20 North 1900 East, Salt Lake City, UT 84132 3201, USA
    Science 300:764. 2003
  13. pmc Gene activation using FLP recombinase in C. elegans
    M Wayne Davis
    Howard Hughes Medical Institute, University of Utah, Salt Lake City, Utah, United States of America
    PLoS Genet 4:e1000028. 2008
    ..We show that we can use this to inactivate synaptic transmission in all neurons or a subset of neurons in a FLP-dependent manner...
  14. ncbi request reprint High-efficiency gene targeting in Drosophila with zinc finger nucleases
    Dana Carroll
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT, USA
    Methods Mol Biol 649:271-80. 2010
    ..A donor DNA carrying desired sequence changes can be incorporated in the injection mix and leads to targeted gene replacement, with particularly good efficiency when the recipient embryos are defective for nonhomologous end joining...
  15. pmc Comparing zinc finger nucleases and transcription activator-like effector nucleases for gene targeting in Drosophila
    Kelly J Beumer
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah 84112
    G3 (Bethesda) 3:1717-25. 2013
    ..Knockout mutations were isolated in several genes in which such alleles were not previously available. TALENs are an effective tool for targeted genome manipulation in Drosophila. ..
  16. ncbi request reprint Using nucleases to stimulate homologous recombination
    Dana Carroll
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, USA
    Methods Mol Biol 262:195-207. 2004
    ..Zinc-finger nucleases have the potential of being targetable to arbitrarily selected sites, owing to the flexibility of zinc finger recognition of DNA...
  17. pmc Targeted mutagenesis using zinc-finger nucleases in Arabidopsis
    Alan Lloyd
    Department of Biology, University of Utah, 257 South 1400 East, Salt Lake City, UT 84112 0840, USA
    Proc Natl Acad Sci U S A 102:2232-7. 2005
    ..These data indicate that ZFNs can form the basis of a highly efficient method for targeted mutagenesis of plant genes...
  18. pmc Induction and repair of zinc-finger nuclease-targeted double-strand breaks in Caenorhabditis elegans somatic cells
    Jason Morton
    Department of Biochemistry, University of Utah School of Medicine, 15 North Medical Drive East, Salt Lake City, UT 84112, USA
    Proc Natl Acad Sci U S A 103:16370-5. 2006
    ..DNA ligase IV is required for efficient end joining, particularly of blunt ends. In its absence, a secondary end-joining pathway relies more heavily on microhomologies in producing deletions...
  19. pmc Targeted chromosomal cleavage and mutagenesis in Drosophila using zinc-finger nucleases
    Marina Bibikova
    Department of Biochemistry, University of Utah School of Medicine, Medical Research and Education Building, 20 North 1900 East, Salt Lake City, UT 84132, USA
    Genetics 161:1169-75. 2002
    ..This approach promises to permit generation of directed mutations in many types of cells and organisms...
  20. pmc Targeted genome engineering techniques in Drosophila
    Kelly J Beumer
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT 84112 5650, United States Electronic address
    Methods 68:29-37. 2014
    ..In lieu of simply providing a protocol for gene targeting, we direct the researcher to resources that will allow access to the latest research in this rapidly evolving field. ..
  21. ncbi request reprint Gene targeting using zinc finger nucleases
    Matthew H Porteus
    Department of Pediatrics, University of Texas Southwestern Medical Center, USA
    Nat Biotechnol 23:967-73. 2005
    ..Future work will be needed to translate these in vitro findings to in vivo applications and to determine whether zinc finger nucleases create undesired genomic instability...

Research Grants2

  1. Chimeric Nucleases for Gene Targeting in Drosophila
    Dana Carroll; Fiscal Year: 2003
    ..To test the generality of the method, it will be applied to a second Drosophila locus, the rosy (ry) gene. ..
  2. Stimulating Gene Targeting with Zinc Finger Nucleases
    Dana Carroll; Fiscal Year: 2004
    ..To demonstrate the generality of this approach to gene targeting, it will be applied to another mouse locus, the Rpo2-1 gene. ..