Roderick Capaldi

Summary

Affiliation: University of Oregon
Country: USA

Publications

  1. ncbi request reprint Investigating mitochondrial redox potential with redox-sensitive green fluorescent protein indicators
    George T Hanson
    Department of Biology, Institute of Molecular Biology, University of Oregon, Eugene, OR 97403 1229, USA
    J Biol Chem 279:13044-53. 2004
  2. ncbi request reprint Immunological approaches to the characterization and diagnosis of mitochondrial disease
    Roderick A Capaldi
    Institute of Molecular Biology, University of Oregon, Eugene, OR 97403 1229, USA
    Mitochondrion 4:417-26. 2004
  3. ncbi request reprint Cross-linking and electron microscopy studies of the structure and functioning of the Escherichia coli ATP synthase
    R A Capaldi
    Institute of Molecular Biology, University of Oregon, Eugene, OR 97403 1229, USA
    J Exp Biol 203:29-33. 2000
  4. ncbi request reprint Mechanism of the F(1)F(0)-type ATP synthase, a biological rotary motor
    Roderick A Capaldi
    Institute of Molecular Biology, University of Oregon, Eugene, OR 97403 1229, USA
    Trends Biochem Sci 27:154-60. 2002
  5. ncbi request reprint A replicating module as the unit of mitochondrial structure and functioning
    Roderick A Capaldi
    Institute of Molecular Biology, University of Oregon, Eugene, OR 97403 1229, USA
    Biochim Biophys Acta 1555:192-5. 2002
  6. ncbi request reprint The mitochondrial inner membrane protein mitofilin exists as a complex with SAM50, metaxins 1 and 2, coiled-coil-helix coiled-coil-helix domain-containing protein 3 and 6 and DnaJC11
    Jing Xie
    Institute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA
    FEBS Lett 581:3545-9. 2007
  7. ncbi request reprint Immunocapture and microplate-based activity measurement of mammalian pyruvate dehydrogenase complex
    Margarita Lib
    Institute of Molecular Biology, University of Oregon, Eugene, Orgon 97403 1229, USA
    Anal Biochem 314:121-7. 2003
  8. ncbi request reprint Energy substrate modulates mitochondrial structure and oxidative capacity in cancer cells
    Rodrigue Rossignol
    Institute of Molecular Biology, University of Oregon, Eugene, Oregon, USA
    Cancer Res 64:985-93. 2004
  9. doi request reprint Lateral-flow immunoassay for detecting drug-induced inhibition of mitochondrial DNA replication and mtDNA-encoded protein synthesis
    Sashi Nadanaciva
    MitoSciences Inc, Eugene, OR 97403, USA
    J Immunol Methods 343:1-12. 2009
  10. ncbi request reprint The subunit composition of the human NADH dehydrogenase obtained by rapid one-step immunopurification
    James Murray
    Department of Molecular Biology, University of Oregon, Eugene 97403, USA
    J Biol Chem 278:13619-22. 2003

Research Grants

  1. ATP SYNTHASE
    Roderick Capaldi; Fiscal Year: 1999
  2. STALK AND FO OF THE ATP SYNTHASE
    Roderick Capaldi; Fiscal Year: 2001
  3. STUDIES OF ATP SYNTHETASE FROM HEART MITOCHONDRIA
    Roderick Capaldi; Fiscal Year: 1980
  4. STUDIES OF ATP SYNTHASE
    Roderick Capaldi; Fiscal Year: 1993
  5. STUDIES OF ATP SYNTHASE
    Roderick Capaldi; Fiscal Year: 2003

Collaborators

Detail Information

Publications32

  1. ncbi request reprint Investigating mitochondrial redox potential with redox-sensitive green fluorescent protein indicators
    George T Hanson
    Department of Biology, Institute of Molecular Biology, University of Oregon, Eugene, OR 97403 1229, USA
    J Biol Chem 279:13044-53. 2004
    ..T. Dooley, T. M. Dore, G. Hanson, W. C. Jackson, S. J. Remington, and R. Y. Tsien, submitted for publication), it is shown that the cytosol of HeLa cells is also unusually reducing but somewhat less so than the mitochondrial matrix...
  2. ncbi request reprint Immunological approaches to the characterization and diagnosis of mitochondrial disease
    Roderick A Capaldi
    Institute of Molecular Biology, University of Oregon, Eugene, OR 97403 1229, USA
    Mitochondrion 4:417-26. 2004
    ..Here we provide details of the mAbs currently available and describe optimized protocols for both immunohistochemistry using patient fibroblasts as well as Western blotting using either cell culture or biopsy material...
  3. ncbi request reprint Cross-linking and electron microscopy studies of the structure and functioning of the Escherichia coli ATP synthase
    R A Capaldi
    Institute of Molecular Biology, University of Oregon, Eugene, OR 97403 1229, USA
    J Exp Biol 203:29-33. 2000
    ..The utility of these two approaches in particular, and the important insights they give into the structure and mechanism of the ATP synthase, are reviewed...
  4. ncbi request reprint Mechanism of the F(1)F(0)-type ATP synthase, a biological rotary motor
    Roderick A Capaldi
    Institute of Molecular Biology, University of Oregon, Eugene, OR 97403 1229, USA
    Trends Biochem Sci 27:154-60. 2002
    ..Evidence for the function of the rotary motor, from structural, genetic and biophysical studies, is reviewed here, and some uncertainties and remaining mysteries of the enzyme mechanism are also discussed...
  5. ncbi request reprint A replicating module as the unit of mitochondrial structure and functioning
    Roderick A Capaldi
    Institute of Molecular Biology, University of Oregon, Eugene, OR 97403 1229, USA
    Biochim Biophys Acta 1555:192-5. 2002
    ..This leads us to propose that a replicating module is the repeating unit of mitochondrial structure. Studies to examine heterogeneity of functioning within the organelle mass are briefly reviewed...
  6. ncbi request reprint The mitochondrial inner membrane protein mitofilin exists as a complex with SAM50, metaxins 1 and 2, coiled-coil-helix coiled-coil-helix domain-containing protein 3 and 6 and DnaJC11
    Jing Xie
    Institute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA
    FEBS Lett 581:3545-9. 2007
    ....
  7. ncbi request reprint Immunocapture and microplate-based activity measurement of mammalian pyruvate dehydrogenase complex
    Margarita Lib
    Institute of Molecular Biology, University of Oregon, Eugene, Orgon 97403 1229, USA
    Anal Biochem 314:121-7. 2003
    ....
  8. ncbi request reprint Energy substrate modulates mitochondrial structure and oxidative capacity in cancer cells
    Rodrigue Rossignol
    Institute of Molecular Biology, University of Oregon, Eugene, Oregon, USA
    Cancer Res 64:985-93. 2004
    ..We compared our finding on HeLa cells with those for nontransformed fibroblasts to help distinguish the regulatory pathways...
  9. doi request reprint Lateral-flow immunoassay for detecting drug-induced inhibition of mitochondrial DNA replication and mtDNA-encoded protein synthesis
    Sashi Nadanaciva
    MitoSciences Inc, Eugene, OR 97403, USA
    J Immunol Methods 343:1-12. 2009
    ..The assay has high reproducibility, requires small amounts of sample, is quantitative, and is able to identify drugs which ultimately lead to a decrease in mtDNA-encoded proteins...
  10. ncbi request reprint The subunit composition of the human NADH dehydrogenase obtained by rapid one-step immunopurification
    James Murray
    Department of Molecular Biology, University of Oregon, Eugene 97403, USA
    J Biol Chem 278:13619-22. 2003
    ..The concordance of data from human and bovine complex I isolated by different procedures adds to the certainty that these novel proteins of seemingly diverse function are a part of complex I...
  11. pmc Cytoskeletal-assisted dynamics of the mitochondrial reticulum in living cells
    Michelle K Knowles
    Department of Chemistry and Materials Science Institute, University of Oregon, Eugene 97403, USA
    Proc Natl Acad Sci U S A 99:14772-7. 2002
    ..This study quantitatively characterizes organelle dynamics in terms of collective cytoskeletal interactions in living cells...
  12. ncbi request reprint Target identification of drug induced mitochondrial toxicity using immunocapture based OXPHOS activity assays
    Sashi Nadanaciva
    MitoSciences Inc, 1850 Millrace Drive, Eugene, OR 97403, USA
    Toxicol In Vitro 21:902-11. 2007
    ..A major advantage of the immunocapture approach is that it can be used throughout drug screening from early compound evaluation to clinical trials...
  13. ncbi request reprint The F(1)F(0) ATP synthase and mitochondrial respiratory chain complexes are present on the plasma membrane of an osteosarcoma cell line: An immunocytochemical study
    Sarah K Yonally
    Institute of Molecular Biology, University of Oregon, Eugene, OR 97403 1229, United States
    Mitochondrion 6:305-14. 2006
    ..A specific mechanism by which the mitochondrion and plasma membrane fuse to deliver organellar proteins is suggested...
  14. ncbi request reprint Small-scale immunopurification of cytochrome c oxidase for a high-throughput multiplexing analysis of enzyme activity and amount
    James Murray
    MitoSciences Inc, Eugene, OR 97403 2095, USA
    Biotechnol Appl Biochem 48:167-78. 2007
    ..We show the utility of this approach by example of the analysis of fibroblasts from patients with COX activity deficiency and the effect of the antiretroviral drug ddC (2',3'-dideoxycytidine) on the biogenesis of the enzyme...
  15. ncbi request reprint Early developmental pathology due to cytochrome c oxidase deficiency is revealed by a new zebrafish model
    Katrina N Baden
    Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403 1229, USA
    J Biol Chem 282:34839-49. 2007
    ..The zebrafish model has revealed tissue-specific responses to COX deficiency and holds promise for discovery of new therapies to treat mitochondrial diseases in humans...
  16. ncbi request reprint Focused proteomics: towards a high throughput monoclonal antibody-based resolution of proteins for diagnosis of mitochondrial diseases
    James Murray
    Institute of Molecular Biology, Department of Biology, University of Oregon, Eugene OR 97403 1229, USA
    Biochim Biophys Acta 1659:206-11. 2004
    ....
  17. ncbi request reprint Quantitative proteomics: the copy number of pyruvate dehydrogenase is more than 10(2)-fold lower than that of complex III in human mitochondria
    James Murray
    Institute of Molecular Biology, University of Oregon, Eugene, OR 97403 1229, USA
    FEBS Lett 529:173-8. 2002
    ..In Rho0 fibroblasts there is a 64% reduction of complex III but the concentration of PDH remains the same as wild-type...
  18. ncbi request reprint An immunocytochemical approach to detection of mitochondrial disorders
    Bonnie J Hanson
    Molecular Probes, Inc, University of Oregon, Eugene, Oregon 97403, USA
    J Histochem Cytochem 50:1281-8. 2002
    ..Immunocytochemical analysis as described here should be considered as an initial screen for mitochondrial disorders by which to direct (and limit) the subsequent enzymatic and genetic tests required to make an unambiguous diagnosis...
  19. ncbi request reprint A functionally active human F1F0 ATPase can be purified by immunocapture from heart tissue and fibroblast cell lines. Subunit structure and activity studies
    Robert Aggeler
    Institute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA
    J Biol Chem 277:33906-12. 2002
    ..The system is therefore suitable to screen patient-derived samples for alterations in amount or functionality of both the F(1)F(0) ATPase and IF(1)...
  20. doi request reprint Screening for the metabolic basis of neurodegeneration: developing a focused proteomic approach
    James Murray
    MitoSciences Inc, Eugene, OR, USA
    Ann N Y Acad Sci 1147:348-57. 2008
    ....
  21. doi request reprint Novel antibody-based strategies for the rapid diagnosis of mitochondrial disease and dysfunction
    Michael F Marusich
    MitoSciences, Inc, 1850 Millrace Dr, Eugene, OR 97403, USA
    Int J Biochem Cell Biol 41:2081-8. 2009
    ....
  22. ncbi request reprint Analysis of steady-state protein phosphorylation in mitochondria using a novel fluorescent phosphosensor dye
    Birte Schulenberg
    Proteomics Section, Molecular Probes, Inc, Eugene, Oregon 97402 9144, USA
    J Biol Chem 278:27251-5. 2003
    ..One novel and prominent phosphoprotein identified in this manner was determined to be the 42-kDa subunit of mitochondrial complex I...
  23. ncbi request reprint The cristal membrane of mitochondria is the principal site of oxidative phosphorylation
    Robert W Gilkerson
    Institute of Molecular Biology, University of Oregon, Eugene, OR 97403 1229, USA
    FEBS Lett 546:355-8. 2003
    ..Our results argue for restricted diffusion of complexes through the cristal junctions and indicate that the mitochondrial cristae comprise a regulated submitochondrial compartment specialized for ATP production...
  24. ncbi request reprint Rapid analysis of mitochondrial DNA depletion by fluorescence in situ hybridization and immunocytochemistry: potential strategies for HIV therapeutic monitoring
    Michael S Janes
    Molecular Probes, Inc, 29851 Willow Creek Road, Eugene, Oregon 97402, USA
    J Histochem Cytochem 52:1011-8. 2004
    ....
  25. ncbi request reprint Characterization of dynamic and steady-state protein phosphorylation using a fluorescent phosphoprotein gel stain and mass spectrometry
    Birte Schulenberg
    Proteomics Department, Molecular Probes, Inc, Eugene, OR 97402, USA
    Electrophoresis 25:2526-32. 2004
    ....
  26. pmc Mitochondrial oxidative phosphorylation protein levels in peripheral blood mononuclear cells correlate with levels in subcutaneous adipose tissue within samples differing by HIV and lipoatrophy status
    Cecilia M Shikuma
    Hawaii AIDS Clinical Research Program, John A Burns School of Medicine, University of Hawaii Manoa, Honolulu, Hawaii 96816, USA
    AIDS Res Hum Retroviruses 24:1255-62. 2008
    ..We conclude that CI and CIV levels in PBMCs correlate to their respective levels in fat and may have utility as surrogate markers of mitochondrial dysfunction in lipoatrophy...
  27. ncbi request reprint Characterization of the human heart mitochondrial proteome
    Steven W Taylor
    MitoKor, 11494 Sorrento Valley Road, San Diego, California 92121, USA
    Nat Biotechnol 21:281-6. 2003
    ..The biochemical roles of 19% of the identified proteins have not been defined. This database of proteins provides a comprehensive resource for the discovery of novel mitochondrial functions and pathways...
  28. ncbi request reprint Angiostatin-like activity of a monoclonal antibody to the catalytic subunit of F1F0 ATP synthase
    Sulene L Chi
    Department of Pathology, Duke University Medical Center, Durham, NC 27710, USA
    Cancer Res 67:4716-24. 2007
    ..Thus, MAb3D5AB1 shows angiostatin-like properties superior to angiostatin and may be exploited in cancer chemotherapy...
  29. pmc An Inhibitor of the F1 subunit of ATP synthase (IF1) modulates the activity of angiostatin on the endothelial cell surface
    Nick R Burwick
    Department of Pathology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 280:1740-5. 2005
    ..These data suggest that there is a relationship between the binding sites of IF1 and angiostatin on ATP synthase and that IF1 could be employed to modulate angiogenesis...
  30. ncbi request reprint Oxidative post-translational modification of tryptophan residues in cardiac mitochondrial proteins
    Steven W Taylor
    MitoKor, San Diego, California 92121, USA
    J Biol Chem 278:19587-90. 2003
    ....
  31. ncbi request reprint An alternative strategy to determine the mitochondrial proteome using sucrose gradient fractionation and 1D PAGE on highly purified human heart mitochondria
    Steven W Taylor
    MitoKor, 11494 Sorrento Valley Road, San Diego California 92121, USA
    J Proteome Res 1:451-8. 2002
    ..The technique described here should also be useful for the identification of new protein-protein associations as exemplified by the validation of a recently discovered complex that involves proteins belonging to the prohibitin family...
  32. ncbi request reprint Rapid purification and mass spectrometric characterization of mitochondrial NADH dehydrogenase (Complex I) from rodent brain and a dopaminergic neuronal cell line
    Birgit Schilling
    Buck Institute for Age Research, Novato, CA 94945, USA
    Mol Cell Proteomics 4:84-96. 2005
    ....

Research Grants31

  1. ATP SYNTHASE
    Roderick Capaldi; Fiscal Year: 1999
    ..These introduced cysteine residues are used to covalently attach reporter groups of conformational changes. In this way, kinetic analyses of conformational changes at various sites in the enzyme complex can be performed. ..
  2. STALK AND FO OF THE ATP SYNTHASE
    Roderick Capaldi; Fiscal Year: 2001
    ..Our idea is that this mobile element links the three different catalytic sites, in turn, with a single proton channel in the F0 to maximize the efficiency of energy coupling. ..
  3. STUDIES OF ATP SYNTHETASE FROM HEART MITOCHONDRIA
    Roderick Capaldi; Fiscal Year: 1980
    ..Special attention will be paid to the arrangement of the DCCD binding proteolipid as this component of the ATP synthetase has been implicated in proton translocation...
  4. STUDIES OF ATP SYNTHASE
    Roderick Capaldi; Fiscal Year: 1993
    ....
  5. STUDIES OF ATP SYNTHASE
    Roderick Capaldi; Fiscal Year: 2003
    ..Studies are planned to examine the enzymatic properties and the assembly of F1F0 in cells of patients harboring subunit 6 mutations. ..