Jose A Cancelas

Summary

Affiliation: University of Cincinnati
Country: USA

Publications

  1. ncbi Infusion of P-Capt prion-filtered red blood cell products demonstrate acceptable in vivo viability and no evidence of neoantigen formation
    Jose A Cancelas
    Hoxworth Blood Center, University of Cincinnati, Cincinnati, Ohio 45267 0055, USA
    Transfusion 51:2228-36. 2011
  2. ncbi Stored red blood cell viability is maintained after treatment with a second-generation S-303 pathogen inactivation process
    Jose A Cancelas
    Hoxworth Blood Center, University of Cincinnati, Cincinnati, Ohio 45267 0055, USA
    Transfusion 51:2367-76. 2011
  3. ncbi In vivo viability of stored red blood cells derived from riboflavin plus ultraviolet light-treated whole blood
    Jose A Cancelas
    Research Division, Hoxworth Blood Center, Cincinnati, Ohio 45267, USA
    Transfusion 51:1460-8. 2011
  4. ncbi On how Rac controls hematopoietic stem cell activity
    J A Cancelas
    Hoxworth Blood Center, University of Cincinnati Academic Health Center, and Stem Cell Program, Division of Experimental Hematology and Cancer Biology, Cincinnati Children s Hospital Medical Center, University of Cincinnati Medical Center, Cincinnati, Ohio, USA
    Transfusion 51:153S-159S. 2011
  5. ncbi Rac2 GTPase deficiency depletes BCR-ABL+ leukemic stem cells and progenitors in vivo
    Amitava Sengupta
    Stem Cell Biology Program, Division of Experimental Hematology and Cancer Biology, Cincinnati Children s Hospital Medical Center, OH
    Blood 116:81-4. 2010
  6. ncbi Neuroblastoma cell lines contain pluripotent tumor initiating cells that are susceptible to a targeted oncolytic virus
    Yonatan Y Mahller
    Division of Hematology and Oncology, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio, United States of America
    PLoS ONE 4:e4235. 2009
  7. ncbi Connexin-43 prevents hematopoietic stem cell senescence through transfer of reactive oxygen species to bone marrow stromal cells
    Eri Taniguchi Ishikawa
    Research Division, Hoxworth Blood Center, University of Cincinnati, Cincinnati, OH 45267, USA
    Proc Natl Acad Sci U S A 109:9071-6. 2012
  8. ncbi Nf1 mutation expands an EGFR-dependent peripheral nerve progenitor that confers neurofibroma tumorigenic potential
    Jon P Williams
    Division of Experimental Hematology and Cancer Biology, Cincinnati Children s Hospital Research Foundation, Cincinnati Children s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229, USA
    Cell Stem Cell 3:658-69. 2008
  9. ncbi Cdc42 critically regulates the balance between myelopoiesis and erythropoiesis
    Linda Yang
    Division of Experimental Hematology, Children s Hospital Medical Center, Cincinnati, OH 45229, USA
    Blood 110:3853-61. 2007
  10. ncbi Rac GTPase isoforms Rac1 and Rac2 play a redundant and crucial role in T-cell development
    Fukun Guo
    Division of Experimental Hematology and Cancer Biology, Children s Hospital Medical Center, OH, USA
    Blood 112:1767-75. 2008

Collaborators

Detail Information

Publications45

  1. ncbi Infusion of P-Capt prion-filtered red blood cell products demonstrate acceptable in vivo viability and no evidence of neoantigen formation
    Jose A Cancelas
    Hoxworth Blood Center, University of Cincinnati, Cincinnati, Ohio 45267 0055, USA
    Transfusion 51:2228-36. 2011
    ..Transmission of variant Creutzfeldt-Jacob disease (vCJD) is a major concern in blood transfusion. The P-Capt filter has been shown to remove around 4 log ID(50) prion infectivity from prion-spiked human red blood cells (RBCs)...
  2. ncbi Stored red blood cell viability is maintained after treatment with a second-generation S-303 pathogen inactivation process
    Jose A Cancelas
    Hoxworth Blood Center, University of Cincinnati, Cincinnati, Ohio 45267 0055, USA
    Transfusion 51:2367-76. 2011
    ..S-303 is a frangible anchor-linker-effector with labile alkylating activity and a robust pathogen reduction profile. This study characterized the viability of RBCs prepared with a second-generation S-303 process and stored for 35 days...
  3. ncbi In vivo viability of stored red blood cells derived from riboflavin plus ultraviolet light-treated whole blood
    Jose A Cancelas
    Research Division, Hoxworth Blood Center, Cincinnati, Ohio 45267, USA
    Transfusion 51:1460-8. 2011
    ..We evaluated stored red blood cell (RBC) metabolic status and viability, in vitro and in vivo, of riboflavin/UV light-treated WB (IMPROVE study)...
  4. ncbi On how Rac controls hematopoietic stem cell activity
    J A Cancelas
    Hoxworth Blood Center, University of Cincinnati Academic Health Center, and Stem Cell Program, Division of Experimental Hematology and Cancer Biology, Cincinnati Children s Hospital Medical Center, University of Cincinnati Medical Center, Cincinnati, Ohio, USA
    Transfusion 51:153S-159S. 2011
    ..The development of small molecule inhibitors with the ability to interfere with Rac GTPase activation offers new therapeutic strategies to manipulate the function of HSC in vivo and ex vivo...
  5. ncbi Rac2 GTPase deficiency depletes BCR-ABL+ leukemic stem cells and progenitors in vivo
    Amitava Sengupta
    Stem Cell Biology Program, Division of Experimental Hematology and Cancer Biology, Cincinnati Children s Hospital Medical Center, OH
    Blood 116:81-4. 2010
    ..In summary, Rac2 deficiency exhausts the LSC pool in vivo through impairment of oncogene-induced proliferation and survival signals...
  6. ncbi Neuroblastoma cell lines contain pluripotent tumor initiating cells that are susceptible to a targeted oncolytic virus
    Yonatan Y Mahller
    Division of Hematology and Oncology, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio, United States of America
    PLoS ONE 4:e4235. 2009
    ..Based on cues from normal stem cells, evidence for tumor populating progenitor cells has been found in a variety of cancers...
  7. ncbi Connexin-43 prevents hematopoietic stem cell senescence through transfer of reactive oxygen species to bone marrow stromal cells
    Eri Taniguchi Ishikawa
    Research Division, Hoxworth Blood Center, University of Cincinnati, Cincinnati, OH 45267, USA
    Proc Natl Acad Sci U S A 109:9071-6. 2012
    ..These results indicate that Cx43 exerts a protective role and regulates the HSC/P ROS content through ROS transfer to the HM, resulting in HSC protection during stress hematopoietic regeneration...
  8. ncbi Nf1 mutation expands an EGFR-dependent peripheral nerve progenitor that confers neurofibroma tumorigenic potential
    Jon P Williams
    Division of Experimental Hematology and Cancer Biology, Cincinnati Children s Hospital Research Foundation, Cincinnati Children s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229, USA
    Cell Stem Cell 3:658-69. 2008
    ..We suggest that expansion of an EGFR-expressing early glial progenitor contributes to neurofibroma formation...
  9. ncbi Cdc42 critically regulates the balance between myelopoiesis and erythropoiesis
    Linda Yang
    Division of Experimental Hematology, Children s Hospital Medical Center, Cincinnati, OH 45229, USA
    Blood 110:3853-61. 2007
    ..Thus, Cdc42 is an essential regulator of the balance between myelopoiesis and erythropoiesis...
  10. ncbi Rac GTPase isoforms Rac1 and Rac2 play a redundant and crucial role in T-cell development
    Fukun Guo
    Division of Experimental Hematology and Cancer Biology, Children s Hospital Medical Center, OH, USA
    Blood 112:1767-75. 2008
    ..Thus, Rac1 and Rac2 have unique roles in CLP production and share a redundant but essential role in later stages of T-cell development by regulating survival and proliferation signals...
  11. ncbi R-Ras and Rac GTPase cross-talk regulates hematopoietic progenitor cell migration, homing, and mobilization
    Xun Shang
    Division of Experimental Hematology and Cancer Biology, Children s Hospital Medical Center, University of Cincinnati, Cincinnati, Ohio 45229, USA
    J Biol Chem 286:24068-78. 2011
    ..These results indicate that R-Ras is a critical regulator of Rac signaling required for HPC migration, homing, and mobilization...
  12. ncbi Signaling and cytoskeletal requirements in erythroblast enucleation
    Diamantis G Konstantinidis
    Cancer and Blood Diseases Institute, Cincinnati Children s Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, OH 45229, USA
    Blood 119:6118-27. 2012
    ....
  13. ncbi Rac GTPases differentially integrate signals regulating hematopoietic stem cell localization
    Jose A Cancelas
    Hoxworth Blood Center, University of Cincinnati Medical Center, 3130 Highland Avenue, Cincinnati, Ohio, 45267, USA
    Nat Med 11:886-91. 2005
    ....
  14. ncbi Inhibition of RhoA GTPase activity enhances hematopoietic stem and progenitor cell proliferation and engraftment
    Gabriel Ghiaur
    Division of Experimental Hematology, Cincinnati Children's Hospital Medical Center, 3333 Burnet Avenue, MLC 7013, Cincinnati, OH 45229, USA
    Blood 108:2087-94. 2006
    ..This mechanism could provide a novel therapeutic target to enhance HSC therapies...
  15. ncbi Fanca-/- hematopoietic stem cells demonstrate a mobilization defect which can be overcome by administration of the Rac inhibitor NSC23766
    Michael D Milsom
    Division of Experimental Hematology, Cincinnati Children s Hospital Research Foundation, Cincinnati, OH, USA
    Haematologica 94:1011-5. 2009
    ..In view of these data, we propose that targeting Rac signaling may enhance G-CSF-induced HSC mobilization in Fanconi anemia...
  16. ncbi Microenvironment determines lineage fate in a human model of MLL-AF9 leukemia
    Junping Wei
    Division of Experimental Hematology, Cincinnati Children s Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, OH 45229, USA
    Cancer Cell 13:483-95. 2008
    ..Targeting the Rac signaling pathway by pharmacologic or genetic means resulted in rapid and specific apoptosis of MLL-AF9 cells, suggesting that the Rac signaling pathway may be a valid therapeutic target in MLL-rearranged AML...
  17. ncbi Rho GTPases and regulation of hematopoietic stem cell localization
    David A Williams
    Division of Experimental Hematology, Cincinnati Children s Research Foundation, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio, USA
    Methods Enzymol 439:365-93. 2008
    ..In addition, it reviews abnormalities of Rho GTPases implicated in human immunohematopoietic diseases and in leukemia/lymphoma...
  18. ncbi FIP1L1/PDGFRalpha synergizes with SCF to induce systemic mastocytosis in a murine model of chronic eosinophilic leukemia/hypereosinophilic syndrome
    Yoshiyuki Yamada
    Division of Allergy and Immunology, Department of Pediatrics, Cincinnati Children s Hospital Medical Center, OH 45229 3039, USA
    Blood 112:2500-7. 2008
    ..The increased proliferation and survival correlated with increased SCF-induced Akt activation. In summary, F/P synergistically promotes MC development, activation, and survival in vivo and in vitro in response to SCF...
  19. ncbi Rac1 and Rac2 GTPases are necessary for early erythropoietic expansion in the bone marrow but not in the spleen
    Theodosia A Kalfa
    Division of Hematology Oncology, Oncology, Cincinnati Children s Research Foundation, Cincinnati Children s Hospital Medical Center, MLC 7015 Cincinnati, OH 45229 3039, USA
    Haematologica 95:27-35. 2010
    ..The role of these Rac GTPases in erythropoiesis has not yet been fully elucidated...
  20. ncbi Rac guanosine triphosphatases represent integrating molecular therapeutic targets for BCR-ABL-induced myeloproliferative disease
    Emily K Thomas
    Division of Experimental Hematology, Cincinnati Children s Research Foundation, Cincinnati Children s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229, USA
    Cancer Cell 12:467-78. 2007
    ..These data demonstrate that Rac is an additional therapeutic target in p210-BCR-ABL-mediated MPD...
  21. ncbi The role of chemokine activation of Rac GTPases in hematopoietic stem cell marrow homing, retention, and peripheral mobilization
    Jose A Cancelas
    Division of Experimental Hematology, Cincinnati Children s Research Foundation, Cincinnati, Ohio 45215, USA
    Exp Hematol 34:976-85. 2006
    ....
  22. ncbi Connexin-43 in the osteogenic BM niche regulates its cellular composition and the bidirectional traffic of hematopoietic stem cells and progenitors
    Daniel González-Nieto
    Division of Experimental Hematology and Cancer Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, OH 45267 0055, USA
    Blood 119:5144-54. 2012
    ..Cx43 controls the cellular content of the BM osteogenic microenvironment and is required for homing of HSC/Ps in myeloablated animals...
  23. ncbi Murine model of hypereosinophilic syndromes/chronic eosinophilic leukemia
    Yoshiyuki Yamada
    Division of Allergy and Immunology, Department of Pediatrics, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio, USA
    Int Arch Allergy Immunol 149:102-7. 2009
    ....
  24. ncbi Atypical protein kinase C (aPKCzeta and aPKClambda) is dispensable for mammalian hematopoietic stem cell activity and blood formation
    Amitava Sengupta
    Division of Experimental Hematology and Cancer Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, OH 45229, USA
    Proc Natl Acad Sci U S A 108:9957-62. 2011
    ....
  25. ncbi Bone marrow connexin-43 expression is critical for hematopoietic regeneration after chemotherapy
    Cynthia A Presley
    Hoxworth Blood Center, University of Cincinnati Medical Center, Cincinnati, OH 45267, USA
    Cell Commun Adhes 12:307-17. 2005
    ..Cx43 expression within the BM appears to be crucial in the development of an efficient response to hematopoietic stress...
  26. ncbi Bmi1 reprograms CML B-lymphoid progenitors to become B-ALL-initiating cells
    Amitava Sengupta
    Stem Cell Program, Division of Experimental Hematology and Cancer Biology, Cincinnati Children s Hospital Medical Center, OH, USA
    Blood 119:494-502. 2012
    ..Our data indicate that BCR-ABL targeting itself is required to eradicate Ph(+)/Bmi1(+) B-ALL-initiating cells and confirm their addiction to BCR-ABL signaling...
  27. ncbi Adhesion, migration, and homing of murine hematopoietic stem cells and progenitors
    Jose A Cancelas
    Division of Experimental Hematology and Cancer Biology, Children s Hospital Medical Center, University of Cincinnati, Cincinnati, OH, USA
    Methods Mol Biol 750:187-96. 2011
    ..In this chapter, we analyze some of the most frequently used assays in our laboratory to explore the ability of HSC to migrate, adhere, and home in in vitro and in vivo assays...
  28. ncbi Rac2-deficient hematopoietic stem cells show defective interaction with the hematopoietic microenvironment and long-term engraftment failure
    Michael Jansen
    Division of Experimental Hematology, Cincinnati Children s Hospital Research Foundation, Cincinnati, OH 45215, USA
    Stem Cells 23:335-46. 2005
    ..Taken together, these data suggest that Rac2(-/-) stem/progenitor cells exhibit abnormal interaction with the hematopoietic microenvironment, which leads to defective long-term engraftment...
  29. ncbi Rho GTPase Cdc42 coordinates hematopoietic stem cell quiescence and niche interaction in the bone marrow
    Linda Yang
    Division of Experimental Hematology and Pathology, Cincinnati Children s Research Foundation, Molecular Developmental Biology Graduate Program, University of Cincinnati, Cincinnati, OH 45229, USA
    Proc Natl Acad Sci U S A 104:5091-6. 2007
    ..Thus, Cdc42 is a critical coordinator of HSC quiescence maintenance and interaction with the BM niche...
  30. ncbi Vav3 collaborates with p190-BCR-ABL in lymphoid progenitor leukemogenesis, proliferation, and survival
    Kyung Hee Chang
    Division of Experimental Hematology, Cincinnati Children s Research Foundation, Cincinnati Children s Hospital Medical Center, Cincinnati, OH
    Blood 120:800-11. 2012
    ..We conclude that Vav3 represents a novel specific molecular leukemic effector for multitarget therapy in p190-BCR-ABL-expressing acute lymphoblastic leukemia...
  31. ncbi Pharmacological targeting of the thrombomodulin-activated protein C pathway mitigates radiation toxicity
    Hartmut Geiger
    Division of Experimental Hematology and Cancer Biology, Cancer and Blood Diseases Institute, Cincinnati Children s Hospital Medical Center, University of Cincinnati, Cincinnati, Ohio, USA
    Nat Med 18:1123-9. 2012
    ..These findings suggest that pharmacologic augmentation of the activity of the Thbd-aPC pathway by recombinant Thbd or aPC might offer a rational approach to the mitigation of tissue injury and lethality caused by ionizing radiation...
  32. ncbi Pharmacological inhibition of EGFR signaling enhances G-CSF-induced hematopoietic stem cell mobilization
    Marnie A Ryan
    Division of Experimental Hematology and Cancer Biology, Cincinnati Children s Hospital Medical Center CCHMC, Cincinnati, Ohio, USA
    Nat Med 16:1141-6. 2010
    ..Our findings reveal a previously unknown signaling pathway regulating stem cell mobilization and provide a new pharmacological approach for improving HSPC mobilization and thereby transplantation outcomes...
  33. ncbi Children are not little adults: just ask their hematopoietic stem cells
    David A Williams
    Division of Experimental Hematology, Cincinnati Children s Research Foundation and Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio 45229, USA
    J Clin Invest 116:2593-6. 2006
    ..Such agents are currently clinically available, suggesting that this approach could be used to improve stem cell transplantation and engraftment...
  34. ncbi A randomized controlled trial evaluating recovery and survival of 6% dimethyl sulfoxide-frozen autologous platelets in healthy volunteers
    Larry J Dumont
    Pathology, The Geisel School of Medicine at Dartmouth, and Pathology, Dartmouth Hitchcock Medical Center, Lebanon, NH 03756, USA
    Transfusion 53:128-37. 2013
    ....
  35. ncbi Perinatal or adult Nf1 inactivation using tamoxifen-inducible PlpCre each cause neurofibroma formation
    Debra A Mayes
    Cincinnati Children s Hospital Medical Center Hoxworth Blood Center, University of Cincinnati, Cincinnati, Ohio, USA
    Cancer Res 71:4675-85. 2011
    ..Together these findings define a tumor suppressor role for Nf1 in the adult and narrow the range of potential neurofibroma-initiating cell populations...
  36. ncbi Rho GTPases in hematopoietic stem cell functions
    Jose A Cancelas
    Division of Experimental Hematology, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio, USA
    Curr Opin Hematol 16:249-54. 2009
    ..Rho GTPases are now known to be crucial in HSCs response to hematopoietic microenvironment cues. This article will review the known HSC functions, which are regulated by Rho GTPases...
  37. ncbi Rho GTPases in hematopoiesis and hemopathies
    James C Mulloy
    Division of Experimental Hematology and Cancer Biology, Cincinnati Children s Hospital Medical Center, University of Cincinnati College of Medicine, OH 45229, USA
    Blood 115:936-47. 2010
    ..In this review we discuss recent genetic studies of Rho GTPases in hematopoiesis and several blood lineages and the implications of Rho GTPase signaling in hematologic malignancies, immune pathology. and anemia...
  38. ncbi Hematopoietic cell regulation by Rac1 and Rac2 guanosine triphosphatases
    Yi Gu
    Division of Experimental Hematology, Cincinnati Children s Hospital Medical Center, Cincinnati, OH 45229, USA
    Science 302:445-9. 2003
    ..Thus, Rac1 and Rac2 regulate unique aspects of hematopoietic development and function...
  39. ncbi Cancer stem cells: a stride towards cancer cure?
    Amitava Sengupta
    Division of Experimental Hematology and Cancer Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio 45229, USA
    J Cell Physiol 225:7-14. 2010
    ....
  40. ncbi The FIP1L1-PDGFRA fusion gene cooperates with IL-5 to induce murine hypereosinophilic syndrome (HES)/chronic eosinophilic leukemia (CEL)-like disease
    Yoshiyuki Yamada
    Division of Allergy and Immunology, Cincinnati Children's Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA
    Blood 107:4071-9. 2006
    ..These results suggest that F/P is not sufficient to induce a HES/CEL-like disease but requires a second event associated with IL-5 overexpression...
  41. ncbi Neurofibroma-associated macrophages play roles in tumor growth and response to pharmacological inhibition
    Carlos E Prada
    Division of Human Genetics, Cincinnati Children s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229, USA
    Acta Neuropathol 125:159-68. 2013
    ..Once tumors are established, they become tumor permissive. Macrophage depletion may result in impaired tumor maintenance and represent a therapeutic strategy for neurofibroma therapy...
  42. ncbi Plexiform and dermal neurofibromas and pigmentation are caused by Nf1 loss in desert hedgehog-expressing cells
    Jianqiang Wu
    Division of Experimental Hematology and Cancer Biology, Cincinnati Children s Research Foundation, Cincinnati Children s Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, OH 45229, USA
    Cancer Cell 13:105-16. 2008
    ..Peripheral nerve and tumors contain transiently proliferating Schwann cells that lose axonal contact, providing insight into early neurofibroma formation. We suggest that timing of Nf1 mutation is critical for neurofibroma formation...
  43. ncbi A rapid method for retrovirus-mediated identification of complementation groups in Fanconi anemia patients
    Saurabh Chandra
    Division of Experimental Hematology and Fanconi Anemia Comprehensive Care Center, Cincinnati Children's Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229, USA
    Mol Ther 12:976-84. 2005
    ..This assay has now been established in a standardized fashion for complementation assignments in FA patients and the subsequent directing of rapid mutation analysis in those patients...
  44. ncbi Tissue inhibitor of metalloproteinase-3 via oncolytic herpesvirus inhibits tumor growth and vascular progenitors
    Yonatan Y Mahller
    Division of Hematology Oncology, Cincinnati Children s Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, Ohio 45229, USA
    Cancer Res 68:1170-9. 2008
    ..These findings support the further development of combined TIMP-3 and oncolytic virotherapy for cancer...
  45. ncbi Stem cell collection and gene transfer in Fanconi anemia
    Patrick F Kelly
    Fanconi Anemia Comprehensive Care Center, Divisions of Experimental Hematology and Hematology Oncology, Cincinnati Children s Hospital Medical Center, University of Cincinnati, Cincinnati, Ohio, USA
    Mol Ther 15:211-9. 2007
    ..Our early experience shows that stem cell collection is well tolerated in FA patients and suggests that collection be considered as early as possible in patients who are potential candidates for future gene transfer trials...