David Campbell

Summary

Affiliation: University of California
Country: USA

Publications

  1. pmc The promoter and transcribed regions of the Leishmania tarentolae spliced leader RNA gene array are devoid of nucleosomes
    Robert A Hitchcock
    Molecular Biology Institute, University of California at Los Angeles, Los Angeles, California 90095 1489, USA
    BMC Microbiol 7:44. 2007
  2. pmc Trypanosoma cruzi mitochondrial maxicircles display species- and strain-specific variation and a conserved element in the non-coding region
    Scott J Westenberger
    Department of Microbiology, Immunology and Molecular Genetics, David Geffen School of Medicine, University of California at Los Angeles 90095, USA
    BMC Genomics 7:60. 2006
  3. pmc Histone acetylations mark origins of polycistronic transcription in Leishmania major
    Sean Thomas
    Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA
    BMC Genomics 10:152. 2009
  4. ncbi request reprint The determinants of Chagas disease: connecting parasite and host genetics
    David A Campbell
    Department of Microbiology, Immunology and Molecular Genetics, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, California 90095 1489, USA
    Curr Mol Med 4:549-62. 2004
  5. ncbi request reprint Transcription in kinetoplastid protozoa: why be normal?
    David A Campbell
    Department of Microbiology, Immunology and Molecular Genetics, University of California at Los Angeles, 609 Charles E Young Drive East, Los Angeles, CA 90095 1489, USA
    Microbes Infect 5:1231-40. 2003
  6. ncbi request reprint Transcription of the kinetoplastid spliced leader RNA gene
    D A Campbell
    Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA 90095 1747, USA
    Parasitol Today 16:78-82. 2000
  7. ncbi request reprint The untranslated regions of genes from Trypanosoma cruzi: perspectives for functional characterization of strains and isolates
    David A Campbell
    Department of Microbiology, Immunology, and Molecular Genetics, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA
    Mem Inst Oswaldo Cruz 102:125-6. 2007
  8. ncbi request reprint Two distinct functional spliced leader RNA gene arrays in Leishmania tarentolae are found in several lizard Leishmania species
    Michael C Yu
    Department of Microbiology, Immunology and Molecular Genetics, School of Medicine, 10833 Le Conte Avenue, University of California, Los Angeles, CA 90095 1747, USA
    Int J Parasitol 32:1411-22. 2002
  9. ncbi request reprint Discovery and barcoding by analysis of spliced leader RNA gene sequences of new isolates of Trypanosomatidae from Heteroptera in Costa Rica and Ecuador
    Dmitri A Maslov
    Department of Biology, University of California, Riverside, California 92521, USA
    J Eukaryot Microbiol 54:57-65. 2007
  10. ncbi request reprint Trypanosoma cruzi 5S rRNA arrays define five groups and indicate the geographic origins of an ancestor of the heterozygous hybrids
    Scott J Westenberger
    Department of Microbiology, Immunology, and Molecular Genetics, University of California at Los Angeles, 609 Charles E Young Drive East, Los Angeles, CA 90095 1489, USA
    Int J Parasitol 36:337-46. 2006

Research Grants

  1. Kinetoplastid SL RNA biogenesis
    David Campbell; Fiscal Year: 2007
  2. EXPRESSION OF THE LEISHMANIA TARENTOLAE SL RNA GENE
    David Campbell; Fiscal Year: 2003
  3. Kinetoplastid SL RNA biogenesis
    David A Campbell; Fiscal Year: 2010

Collaborators

  • Sean Thomas
  • Nancy R Sturm
  • Janusz M Bujnicki
  • Scott J Westenberger
  • Dmitri A Maslov
  • Marcin Feder
  • Christian Barnabe
  • Aldons Jake Lusis
  • Keith Gull
  • Marcela Ferella
  • Senjie Lin
  • PETER JOHN MYLER
  • Richard McCulloch
  • Frederic Bringaud
  • David Horn
  • Ivan Fiala
  • Marilyn Parsons
  • Erik Arner
  • Jesse R Zamudio
  • Gusti M Zeiner
  • Bidyottam Mittra
  • Robert A Hitchcock
  • Julius Lukes
  • Silvie Foldynová-Trantírková
  • Tereza Cristina Orlando
  • Huan Zhang
  • Kum Kum Bhasin
  • Najib M El-Sayed
  • Sergei A Podlipaev
  • Otávio T Nóbrega
  • Tereza C Orlando
  • Michael C Yu
  • Lucile M Floeter-Winter
  • James A Wohlschlegel
  • Arnab Chattopadhyay
  • Edward Darzynkiewicz
  • Janusz Stepinski
  • Lucile Maria Floeter-Winter
  • Lilibeth Miranda
  • Mario Gustavo Mayer
  • Yubo Hou
  • Terry Gaasterland
  • Janet M Yu
  • Diana Shih
  • Aaron Tward
  • Claire M Fraser
  • Peter Burton
  • Hernan Lorenzi
  • Lihua Hou
  • Gustavo C Cerqueira
  • Philip Attipoe
  • Tamara Feldblyum
  • Alan McKenna
  • Kimberly Edwards
  • Pauline N Ward
  • Hean Koo
  • Daniel O Sanchez
  • Daniella C Bartholomeu
  • Anjana J Simpson
  • Eithon Cadag
  • Brian Haas
  • Lena Aslund
  • Bill Wickstead
  • Ellen Kindlund
  • Ellen Sisk
  • Carole Branche
  • Yiting Huang
  • Esteban Bontempi
  • Steven L Salzberg
  • Elizabeth A Worthey
  • Hamid Darban
  • Tin Louie
  • Elisabet Caler
  • Santuza Teixeira
  • Jyoti Shetty
  • Bjorn Andersson
  • Daniela Lacerda
  • Arthur L Delcher
  • Yumi Mizuno
  • Martin Pentony
  • Rick Tarleton
  • Siri Nelson
  • Laura Robertson
  • Atashi Anupama
  • Martti T Tammi
  • Anh Nhi Tran
  • Ulf Pettersson
  • Stephen Ochaya
  • Gholam Fazelina
  • Christy Vogt

Detail Information

Publications35

  1. pmc The promoter and transcribed regions of the Leishmania tarentolae spliced leader RNA gene array are devoid of nucleosomes
    Robert A Hitchcock
    Molecular Biology Institute, University of California at Los Angeles, Los Angeles, California 90095 1489, USA
    BMC Microbiol 7:44. 2007
    ..To explore these possibilities we examined the nucleoplasmic localization of the SL RNA genes as well as their nucleosomal architecture...
  2. pmc Trypanosoma cruzi mitochondrial maxicircles display species- and strain-specific variation and a conserved element in the non-coding region
    Scott J Westenberger
    Department of Microbiology, Immunology and Molecular Genetics, David Geffen School of Medicine, University of California at Los Angeles 90095, USA
    BMC Genomics 7:60. 2006
    ..Many maxicircle-encoded mRNAs cannot be translated without this post-transcriptional sequence modification...
  3. pmc Histone acetylations mark origins of polycistronic transcription in Leishmania major
    Sean Thomas
    Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA
    BMC Genomics 10:152. 2009
    ..However, the mechanism by which Kinetoplastidae initiate transcription is unclear, and promoter sequences are undefined...
  4. ncbi request reprint The determinants of Chagas disease: connecting parasite and host genetics
    David A Campbell
    Department of Microbiology, Immunology and Molecular Genetics, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, California 90095 1489, USA
    Curr Mol Med 4:549-62. 2004
    ..At some point in the future, these disparate areas of study will have to come together. It is to be hoped that this scientific fusion will result in better prognosis and treatment of Chagas disease...
  5. ncbi request reprint Transcription in kinetoplastid protozoa: why be normal?
    David A Campbell
    Department of Microbiology, Immunology and Molecular Genetics, University of California at Los Angeles, 609 Charles E Young Drive East, Los Angeles, CA 90095 1489, USA
    Microbes Infect 5:1231-40. 2003
    ..Here, we present a broad overview of what is known about transcription in the kinetoplastids and what has yet to be determined...
  6. ncbi request reprint Transcription of the kinetoplastid spliced leader RNA gene
    D A Campbell
    Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA 90095 1747, USA
    Parasitol Today 16:78-82. 2000
    ..In this article, David Campbell, Nancy Sturm and Michael Yu review the field of kinetoplastid SL RNA gene transcription, address past ..
  7. ncbi request reprint The untranslated regions of genes from Trypanosoma cruzi: perspectives for functional characterization of strains and isolates
    David A Campbell
    Department of Microbiology, Immunology, and Molecular Genetics, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA
    Mem Inst Oswaldo Cruz 102:125-6. 2007
  8. ncbi request reprint Two distinct functional spliced leader RNA gene arrays in Leishmania tarentolae are found in several lizard Leishmania species
    Michael C Yu
    Department of Microbiology, Immunology and Molecular Genetics, School of Medicine, 10833 Le Conte Avenue, University of California, Los Angeles, CA 90095 1747, USA
    Int J Parasitol 32:1411-22. 2002
    ....
  9. ncbi request reprint Discovery and barcoding by analysis of spliced leader RNA gene sequences of new isolates of Trypanosomatidae from Heteroptera in Costa Rica and Ecuador
    Dmitri A Maslov
    Department of Biology, University of California, Riverside, California 92521, USA
    J Eukaryot Microbiol 54:57-65. 2007
    ..Investigations of trypanosomatid diversity, host-specificity, and biogeography have become feasible using the approach described herein...
  10. ncbi request reprint Trypanosoma cruzi 5S rRNA arrays define five groups and indicate the geographic origins of an ancestor of the heterozygous hybrids
    Scott J Westenberger
    Department of Microbiology, Immunology, and Molecular Genetics, University of California at Los Angeles, 609 Charles E Young Drive East, Los Angeles, CA 90095 1489, USA
    Int J Parasitol 36:337-46. 2006
    ....
  11. doi request reprint Alternative lifestyles: the population structure of Trypanosoma cruzi
    Nancy R Sturm
    Department of Microbiology, Immunology and Molecular Genetics, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, CA 90095, USA
    Acta Trop 115:35-43. 2010
    ..Thus, the total number of alleles may increase to five in another few million years...
  12. pmc Two hybridization events define the population structure of Trypanosoma cruzi
    Scott J Westenberger
    Department of Microbiology, Immunology and Molecular Genetics, David Geffen School of Medicine, University of California, Los Angeles, 90095, USA
    Genetics 171:527-43. 2005
    ..The second hybridization between DTU IIb and IIc strains that generated DTUs IId and IIe resulted in extensive heterozygosity with subsequent recombination of parental genotypes...
  13. ncbi request reprint Intragenomic spliced leader RNA array analysis of kinetoplastids reveals unexpected transcribed region diversity in Trypanosoma cruzi
    Sean Thomas
    Molecular Biology Institute, University of California, Los Angeles, CA, USA
    Gene 352:100-8. 2005
    ....
  14. pmc Exportin 1 mediates nuclear export of the kinetoplastid spliced leader RNA
    Gusti M Zeiner
    Department of Microbiology, University of California at Los Angeles, Los Angeles, California 90095 1489, USA
    Eukaryot Cell 2:222-30. 2003
    ..This study demonstrates spatial and mechanistic similarities between the posttranscriptional trafficking of the kinetoplastid protozoan SL RNA and the metazoan cis-spliceosomal small nuclear RNAs...
  15. pmc 3'-End polishing of the kinetoplastid spliced leader RNA is performed by SNIP, a 3'-->5' exonuclease with a Motley assortment of small RNA substrates
    Gusti M Zeiner
    Department of Microbiology, Immunology, and Molecular Genetics, University of California at Los Angeles, 609 Charles E Young Dr East, Los Angeles, CA 90095 1489, USA
    Mol Cell Biol 24:10390-6. 2004
    ..We speculate that SNIP is part of an organized nucleoplasmic machinery responsible for processing of SL RNA...
  16. ncbi request reprint The TbMTr1 spliced leader RNA cap 1 2'-O-ribose methyltransferase from Trypanosoma brucei acts with substrate specificity
    Bidyottam Mittra
    Department of Microbiology, Immunology and Molecular Genetics, David Geffen School of Medicine, University of California, Los Angeles, California 90095, USA
    J Biol Chem 283:3161-72. 2008
    ..With similarities to human cap 1 methyltransferase activity, TbMTr1 is an excellent model for higher eukaryotic cap 1 methyltransferases and the consequences of cap 1 modification...
  17. pmc Complete cap 4 formation is not required for viability in Trypanosoma brucei
    Jesse R Zamudio
    Department of Microbiology, Immunology and Molecular Genetics, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, CA 90095 1489, USA
    Eukaryot Cell 5:905-15. 2006
    ..As mature cap 4 is dispensable for translation, cap 1 modifications and/or SL sequences are implicated in ribosomal interaction...
  18. pmc The 2'-O-ribose methyltransferase for cap 1 of spliced leader RNA and U1 small nuclear RNA in Trypanosoma brucei
    Jesse R Zamudio
    Department of Microbiology, Immunology and Molecular Genetics, David Geffen School of Medicine, 609 Charles E Young Drive East, University of California at Los Angeles, Los Angeles, CA 90095 1489, USA
    Mol Cell Biol 27:6084-92. 2007
    ..Accumulation of 3'-extended SL RNA substrate indicates a delay in processing and suggests a synergistic role for cap 1 in maturation...
  19. pmc Trypanosoma brucei spliced leader RNA maturation by the cap 1 2'-O-ribose methyltransferase and SLA1 H/ACA snoRNA pseudouridine synthase complex
    Jesse R Zamudio
    Department of Microbiology, Immunology, and Molecular Genetics, University of California at Los Angeles, Los Angeles, CA 90095 1489, USA
    Mol Cell Biol 29:1202-11. 2009
    ..The TbMTr1 complex specializes the SLA1 H/ACA snoRNP for efficient processing of multiple modifications on the SL RNA substrate...
  20. ncbi request reprint A non-universal transcription factor? The Leishmania tarentolae TATA box-binding protein LtTBP associates with a subset of promoters
    Sean Thomas
    Molecular Biology Institute, University of California, Los Angeles, CA 90095 1489, USA
    Int J Parasitol 36:1217-26. 2006
    ..The LtTBP appears to interact with a subset of promoters in kinetoplastids with an affinity for short transcription units...
  21. ncbi request reprint Evidence for multiple hybrid groups in Trypanosoma cruzi
    Nancy R Sturm
    Department of Microbiology, Immunology and Molecular Genetics, University of California at Los Angeles, 609 Charles E Young Drive East, Los Angeles, CA 90095 1489, USA
    Int J Parasitol 33:269-79. 2003
    ..These data demonstrate for the first time that IIa/IIc are hybrid, consistent with the hypothesis that genetic recombination has occurred more than once within the T. cruzi lines...
  22. pmc SmD1 is required for spliced leader RNA biogenesis
    Gusti M Zeiner
    Department of Microbiology, Immunology and Molecular Genetics, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, California 90095 1489, USA
    Eukaryot Cell 3:241-4. 2004
    ....
  23. pmc Hypermethylated cap 4 maximizes Trypanosoma brucei translation
    Jesse R Zamudio
    Department of Microbiology, Immunology and Molecular Genetics, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, CA 90095 1489, USA
    Mol Microbiol 72:1100-10. 2009
    ..A minimal level of mRNA cap ribose methylation is essential for trypanosome viability, providing the first functional role for the cap 4...
  24. ncbi request reprint The Leishmania tarentolae spliced leader contains determinants for association with polysomes
    Gusti M Zeiner
    Department of Microbiology, Immunology, and Molecular Genetics, University of California at Los Angeles, Los Angeles, California 90095 1489, USA
    J Biol Chem 278:38269-75. 2003
    ..This is the first report demonstrating that the spliced leader contains critical structural or sequence determinants for association with polysomes and, hence, translation...
  25. ncbi request reprint The 3' termini of small RNAs in Trypanosoma brucei
    Robert A Hitchcock
    Department of Microbiology, Immunology, and Molecular Genetics, David Geffen School of Medicine, University of California at Los Angeles, 4825 Molecular Sciences Building, 609 Charles E Young Drive, 90095 1489, USA
    FEMS Microbiol Lett 236:73-8. 2004
    ..Multiple mechanisms and structural features are likely to influence 3' end maturation of RNAs...
  26. ncbi request reprint Trypanosoma congolense: paraoxonase 1 prolongs survival of infected mice
    Kum Kum Bhasin
    Department of Medicine, University of California, Los Angeles, CA 90095, USA
    Exp Parasitol 114:240-5. 2006
    ..Our data are consistent with an in vivo role of HDL protection against trypanosome infection...
  27. ncbi request reprint Intergenic and external transcribed spacers of ribosomal RNA genes in lizard-infecting Leishmania: molecular structure and phylogenetic relationship to mammal-infecting Leishmania in the subgenus Leishmania (Leishmania)
    Tereza C Orlando
    Departamento de Parasitologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Sao Paulo, SP, 05508 900, Brasil
    Mem Inst Oswaldo Cruz 97:695-701. 2002
    ....
  28. ncbi request reprint The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease
    Najib M El-Sayed
    Department of Parasite Genomics, Institute for Genomic Research, Rockville, MD 20850, USA
    Science 309:409-15. 2005
    ....
  29. ncbi request reprint Diversity of insect trypanosomatids assessed from the spliced leader RNA and 5S rRNA genes and intergenic regions
    Sergei A Podlipaev
    Institute of Parasitology, Czech Academy of Sciences, Ceske Budejovice, Czech Republic
    J Eukaryot Microbiol 51:283-90. 2004
    ..Our data revealed additional discrepancies between molecular phylogenetic data and cell morphology, rendering current trypanosomatid taxonomy unreliable...
  30. ncbi request reprint The Trypanosoma brucei La protein is a candidate poly(U) shield that impacts spliced leader RNA maturation and tRNA intron removal
    Silvie Foldynová-Trantírková
    Institute of Parasitology, Czech Academy of Sciences, Faculty of Biology, University of South Bohemia, 37005 Ceske Budejovice, Czech Republic
    Int J Parasitol 35:359-66. 2005
    ....
  31. ncbi request reprint Rational sub-division of plant trypanosomes (Phytomonas spp.) based on minicircle conserved region analysis
    Nancy R Sturm
    CIRAD, UPR29 Etiologie Dépérissements, Département Systèmes Biologiques, TA A 29 F, Campus International de Baillarguet, 34398 Montpellier Cedex 5, France
    Infect Genet Evol 7:570-6. 2007
    ..The remainder of the isolates fell into a heterogeneous cluster. At least eight different groups in the plant trypanosomatids were identified...
  32. pmc Spliced leader RNA trans-splicing in dinoflagellates
    Huan Zhang
    Department of Marine Sciences, University of Connecticut, 1080 Shennecossett Road, Groton, CT 06340, USA
    Proc Natl Acad Sci U S A 104:4618-23. 2007
    ..AF 512889, DQ 864761-DQ 864971, DQ 867053-DQ 867070, DQ 884413-DQ 884451, EF 133854-EF 133905, EF 133961-EF 134003, EF 134083-EF 134402, EF 141835, and EF 143070-EF 143105)...
  33. ncbi request reprint RNA polymerase I promoter and splice acceptor site recognition affect gene expression in non-pathogenic Leishmania species
    Tereza Cristina Orlando
    Departamento de Fisiologia, Instituto de Biociencias, Sao Paulo, SP, 05508 900, Brazil
    Mem Inst Oswaldo Cruz 102:891-4. 2007
    ..In relation to the promoter sequence the reporter expression was higher in heterologous lizard-infecting species than in the homologous L. tarentolae or in the mammalian-infecting L. (Leishmania) amazonensis...
  34. ncbi request reprint Etiologics ltd
    David A Campbell
    Etiologics Ltd, Medical Research Council, Harwell, Oxfordshire, OX11 0RD, UK
    Pharmacogenomics 4:351-7. 2003
    ..Etiologics also offers partners access to its ENU technology and/or preclinical respiratory models/expertise to enable them to identify/validate new targets and enhance the value of in-house programs...
  35. ncbi request reprint HygR and PurR plasmid vectors for episomal transfection of Trypanosoma cruzi
    Otávio T Nóbrega
    Laboratorio Multidisciplinar de Pesquisa em Doenca de Chagas, Faculdade de Medicina, Universidade de Brasilia, Brasilia, DF, Brazil
    Mem Inst Oswaldo Cruz 99:513-6. 2004
    ..The ability to modify pHygD and the availability of the complete DNA sequence make these plasmids useful tools for the genetic manipulation of T. cruzi...

Research Grants15

  1. Kinetoplastid SL RNA biogenesis
    David Campbell; Fiscal Year: 2007
    ..Specific inhibition of the trans-splicing pathway is lethal to the cell and thus a prime target for anti-parasite intervention. ..
  2. EXPRESSION OF THE LEISHMANIA TARENTOLAE SL RNA GENE
    David Campbell; Fiscal Year: 2003
    ..These unique features represent potential targets for selective therapeutic intervention. ..
  3. Kinetoplastid SL RNA biogenesis
    David A Campbell; Fiscal Year: 2010
    ..The goal of this study is to continue the identification of spliced leader specific processing components that have the potential to be specific targets for anti-parasite chemotherapy. ..