Research Topics
| David CampbellSummaryAffiliation: University of California Country: USA Publications
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Detail Information
Publications
The promoter and transcribed regions of the Leishmania tarentolae spliced leader RNA gene array are devoid of nucleosomesRobert A Hitchcock
Molecular Biology Institute, University of California at Los Angeles, Los Angeles, California 90095 1489, USA
BMC Microbiol 7:44. 2007..To explore these possibilities we examined the nucleoplasmic localization of the SL RNA genes as well as their nucleosomal architecture...- Trypanosoma cruzi mitochondrial maxicircles display species- and strain-specific variation and a conserved element in the non-coding regionScott J Westenberger
Department of Microbiology, Immunology and Molecular Genetics, David Geffen School of Medicine, University of California at Los Angeles 90095, USA
BMC Genomics 7:60. 2006..Many maxicircle-encoded mRNAs cannot be translated without this post-transcriptional sequence modification... - Histone acetylations mark origins of polycistronic transcription in Leishmania majorSean Thomas
Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA
BMC Genomics 10:152. 2009..However, the mechanism by which Kinetoplastidae initiate transcription is unclear, and promoter sequences are undefined... 
The determinants of Chagas disease: connecting parasite and host geneticsDavid A Campbell
Department of Microbiology, Immunology and Molecular Genetics, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, California 90095 1489, USA
Curr Mol Med 4:549-62. 2004..At some point in the future, these disparate areas of study will have to come together. It is to be hoped that this scientific fusion will result in better prognosis and treatment of Chagas disease...- Transcription in kinetoplastid protozoa: why be normal?David A Campbell
Department of Microbiology, Immunology and Molecular Genetics, University of California at Los Angeles, 609 Charles E Young Drive East, Los Angeles, CA 90095 1489, USA
Microbes Infect 5:1231-40. 2003..Here, we present a broad overview of what is known about transcription in the kinetoplastids and what has yet to be determined... - Transcription of the kinetoplastid spliced leader RNA geneD A Campbell
Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA 90095 1747, USA
Parasitol Today 16:78-82. 2000..In this article, David Campbell, Nancy Sturm and Michael Yu review the field of kinetoplastid SL RNA gene transcription, address past .. - The untranslated regions of genes from Trypanosoma cruzi: perspectives for functional characterization of strains and isolatesDavid A Campbell
Department of Microbiology, Immunology, and Molecular Genetics, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA
Mem Inst Oswaldo Cruz 102:125-6. 2007 - Two distinct functional spliced leader RNA gene arrays in Leishmania tarentolae are found in several lizard Leishmania speciesMichael C Yu
Department of Microbiology, Immunology and Molecular Genetics, School of Medicine, 10833 Le Conte Avenue, University of California, Los Angeles, CA 90095-1747, USA
Int J Parasitol 32:1411-22. 2002.... - Discovery and barcoding by analysis of spliced leader RNA gene sequences of new isolates of Trypanosomatidae from Heteroptera in Costa Rica and EcuadorDmitri A Maslov
Department of Biology, University of California, Riverside, California 92521, USA
J Eukaryot Microbiol 54:57-65. 2007..Investigations of trypanosomatid diversity, host-specificity, and biogeography have become feasible using the approach described herein... - Trypanosoma cruzi 5S rRNA arrays define five groups and indicate the geographic origins of an ancestor of the heterozygous hybridsScott J Westenberger
Department of Microbiology, Immunology, and Molecular Genetics, University of California at Los Angeles, 609 Charles E. Young Drive East, Los Angeles, CA 90095-1489, USA
Int J Parasitol 36:337-46. 2006.... 
Alternative lifestyles: the population structure of Trypanosoma cruziNancy R Sturm
Department of Microbiology, Immunology and Molecular Genetics, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, CA 90095, USA
Acta Trop 115:35-43. 2010..Thus, the total number of alleles may increase to five in another few million years...- Two hybridization events define the population structure of Trypanosoma cruziScott J Westenberger
Department of Microbiology, Immunology and Molecular Genetics, David Geffen School of Medicine, University of California, Los Angeles, 90095, USA
Genetics 171:527-43. 2005..The second hybridization between DTU IIb and IIc strains that generated DTUs IId and IIe resulted in extensive heterozygosity with subsequent recombination of parental genotypes... - Intragenomic spliced leader RNA array analysis of kinetoplastids reveals unexpected transcribed region diversity in Trypanosoma cruziSean Thomas
Molecular Biology Institute, University of California, Los Angeles, CA, USA
Gene 352:100-8. 2005.... - Exportin 1 mediates nuclear export of the kinetoplastid spliced leader RNAGusti M Zeiner
Department of Microbiology, University of California at Los Angeles, Los Angeles, California 90095 1489, USA
Eukaryot Cell 2:222-30. 2003..This study demonstrates spatial and mechanistic similarities between the posttranscriptional trafficking of the kinetoplastid protozoan SL RNA and the metazoan cis-spliceosomal small nuclear RNAs... - 3'-End polishing of the kinetoplastid spliced leader RNA is performed by SNIP, a 3'-->5' exonuclease with a Motley assortment of small RNA substratesGusti M Zeiner
Department of Microbiology, Immunology, and Molecular Genetics, University of California at Los Angeles, 609 Charles E. Young Dr. East, Los Angeles, CA 90095-1489, USA
Mol Cell Biol 24:10390-6. 2004..We speculate that SNIP is part of an organized nucleoplasmic machinery responsible for processing of SL RNA... - The TbMTr1 spliced leader RNA cap 1 2'-O-ribose methyltransferase from Trypanosoma brucei acts with substrate specificityBidyottam Mittra
Department of Microbiology, Immunology and Molecular Genetics, David Geffen School of Medicine, University of California, Los Angeles, California 90095, USA
J Biol Chem 283:3161-72. 2008..With similarities to human cap 1 methyltransferase activity, TbMTr1 is an excellent model for higher eukaryotic cap 1 methyltransferases and the consequences of cap 1 modification... - Complete cap 4 formation is not required for viability in Trypanosoma bruceiJesse R Zamudio
Department of Microbiology, Immunology and Molecular Genetics, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, CA 90095-1489, USA
Eukaryot Cell 5:905-15. 2006..As mature cap 4 is dispensable for translation, cap 1 modifications and/or SL sequences are implicated in ribosomal interaction... - The 2'-O-ribose methyltransferase for cap 1 of spliced leader RNA and U1 small nuclear RNA in Trypanosoma bruceiJesse R Zamudio
Department of Microbiology, Immunology and Molecular Genetics, David Geffen School of Medicine, 609 Charles E Young Drive East, University of California at Los Angeles, Los Angeles, CA 90095 1489, USA
Mol Cell Biol 27:6084-92. 2007..Accumulation of 3'-extended SL RNA substrate indicates a delay in processing and suggests a synergistic role for cap 1 in maturation... - Trypanosoma brucei spliced leader RNA maturation by the cap 1 2'-O-ribose methyltransferase and SLA1 H/ACA snoRNA pseudouridine synthase complexJesse R Zamudio
Department of Microbiology, Immunology, and Molecular Genetics, University of California at Los Angeles, Los Angeles, CA 90095 1489, USA
Mol Cell Biol 29:1202-11. 2009..The TbMTr1 complex specializes the SLA1 H/ACA snoRNP for efficient processing of multiple modifications on the SL RNA substrate... - A non-universal transcription factor? The Leishmania tarentolae TATA box-binding protein LtTBP associates with a subset of promotersSean Thomas
Molecular Biology Institute, University of California, Los Angeles, CA 90095 1489, USA
Int J Parasitol 36:1217-26. 2006..The LtTBP appears to interact with a subset of promoters in kinetoplastids with an affinity for short transcription units... - Evidence for multiple hybrid groups in Trypanosoma cruziNancy R Sturm
Department of Microbiology, Immunology and Molecular Genetics, University of California at Los Angeles, 609 Charles E. Young Drive East, Los Angeles, CA 90095-1489, USA
Int J Parasitol 33:269-79. 2003..These data demonstrate for the first time that IIa/IIc are hybrid, consistent with the hypothesis that genetic recombination has occurred more than once within the T. cruzi lines... - SmD1 is required for spliced leader RNA biogenesisGusti M Zeiner
Department of Microbiology, Immunology and Molecular Genetics, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, California 90095-1489, USA
Eukaryot Cell 3:241-4. 2004.... - Hypermethylated cap 4 maximizes Trypanosoma brucei translationJesse R Zamudio
Department of Microbiology, Immunology and Molecular Genetics, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, CA 90095 1489, USA
Mol Microbiol 72:1100-10. 2009..A minimal level of mRNA cap ribose methylation is essential for trypanosome viability, providing the first functional role for the cap 4... - The Leishmania tarentolae spliced leader contains determinants for association with polysomesGusti M Zeiner
Department of Microbiology, Immunology, and Molecular Genetics, University of California at Los Angeles, Los Angeles, California 90095-1489, USA
J Biol Chem 278:38269-75. 2003..This is the first report demonstrating that the spliced leader contains critical structural or sequence determinants for association with polysomes and, hence, translation... - The 3' termini of small RNAs in Trypanosoma bruceiRobert A Hitchcock
Department of Microbiology, Immunology, and Molecular Genetics, David Geffen School of Medicine, University of California at Los Angeles, 4825 Molecular Sciences Building, 609 Charles E. Young Drive, 90095-1489, USA
FEMS Microbiol Lett 236:73-8. 2004..Multiple mechanisms and structural features are likely to influence 3' end maturation of RNAs... - Trypanosoma congolense: paraoxonase 1 prolongs survival of infected miceKum Kum Bhasin
Department of Medicine, University of California, Los Angeles, CA 90095, USA
Exp Parasitol 114:240-5. 2006..Our data are consistent with an in vivo role of HDL protection against trypanosome infection... - Intergenic and external transcribed spacers of ribosomal RNA genes in lizard-infecting Leishmania: molecular structure and phylogenetic relationship to mammal-infecting Leishmania in the subgenus Leishmania (Leishmania)Tereza C Orlando
Departamento de Parasitologia, , , , SP, 05508-900, Brasil
Mem Inst Oswaldo Cruz 97:695-701. 2002.... - The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas diseaseNajib M El-Sayed
Department of Parasite Genomics, Institute for Genomic Research, Rockville, MD 20850, USA
Science 309:409-15. 2005.... - Diversity of insect trypanosomatids assessed from the spliced leader RNA and 5S rRNA genes and intergenic regionsSergei A Podlipaev
Institute of Parasitology, Czech Academy of Sciences, , Czech Republic
J Eukaryot Microbiol 51:283-90. 2004..Our data revealed additional discrepancies between molecular phylogenetic data and cell morphology, rendering current trypanosomatid taxonomy unreliable... - The Trypanosoma brucei La protein is a candidate poly(U) shield that impacts spliced leader RNA maturation and tRNA intron removalSilvie Foldynová-Trantírková
Institute of Parasitology, Czech Academy of Sciences, Faculty of Biology, University of South Bohemia, 37005 Ceske Budejovice, Czech Republic
Int J Parasitol 35:359-66. 2005.... - Rational sub-division of plant trypanosomes (Phytomonas spp.) based on minicircle conserved region analysisNancy R Sturm
CIRAD, UPR29 Etiologie Dépérissements, Département Systèmes Biologiques, TA A 29 F, Campus International de Baillarguet, 34398 Montpellier Cedex 5, France
Infect Genet Evol 7:570-6. 2007..The remainder of the isolates fell into a heterogeneous cluster. At least eight different groups in the plant trypanosomatids were identified... - Spliced leader RNA trans-splicing in dinoflagellatesHuan Zhang
Department of Marine Sciences, University of Connecticut, 1080 Shennecossett Road, Groton, CT 06340, USA
Proc Natl Acad Sci U S A 104:4618-23. 2007..AF 512889, DQ 864761-DQ 864971, DQ 867053-DQ 867070, DQ 884413-DQ 884451, EF 133854-EF 133905, EF 133961-EF 134003, EF 134083-EF 134402, EF 141835, and EF 143070-EF 143105)... - RNA polymerase I promoter and splice acceptor site recognition affect gene expression in non-pathogenic Leishmania speciesTereza Cristina Orlando
Departamento de Fisiologia, Instituto de Biociencias, Sao Paulo, SP, 05508 900, Brazil
Mem Inst Oswaldo Cruz 102:891-4. 2007..In relation to the promoter sequence the reporter expression was higher in heterologous lizard-infecting species than in the homologous L. tarentolae or in the mammalian-infecting L. (Leishmania) amazonensis... - Etiologics ltdDavid A Campbell
Etiologics Ltd, Medical Research Council, Harwell, Oxfordshire, OX11 0RD, UK
Pharmacogenomics 4:351-7. 2003..Etiologics also offers partners access to its ENU technology and/or preclinical respiratory models/expertise to enable them to identify/validate new targets and enhance the value of in-house programs... - HygR and PurR plasmid vectors for episomal transfection of Trypanosoma cruziOtávio T Nóbrega
Laboratorio Multidisciplinar de Pesquisa em Doenca de Chagas, Faculdade de Medicina, Universidade de Brasilia, Brasilia, DF, Brazil
Mem Inst Oswaldo Cruz 99:513-6. 2004..The ability to modify pHygD and the availability of the complete DNA sequence make these plasmids useful tools for the genetic manipulation of T. cruzi...
Research Grants
- Kinetoplastid SL RNA biogenesisDavid Campbell; Fiscal Year: 2007..Specific inhibition of the trans-splicing pathway is lethal to the cell and thus a prime target for anti-parasite intervention. ..
- EXPRESSION OF THE LEISHMANIA TARENTOLAE SL RNA GENEDavid Campbell; Fiscal Year: 2003..These unique features represent potential targets for selective therapeutic intervention. ..
- Kinetoplastid SL RNA biogenesisDavid A Campbell; Fiscal Year: 2010..The goal of this study is to continue the identification of spliced leader specific processing components that have the potential to be specific targets for anti-parasite chemotherapy. ..