Research Topics
| Igor A ButovichSummaryAffiliation: University of Texas Southwestern Medical Center Country: USA Publications
Research Grants
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Detail Information
Publications
Comparative HPLC-MS analysis of canine and human meibomian lipidomes: many similarities, a few differencesIgor A Butovich
University of Texas Southwestern Medical Center, Department of Ophthalmology and the Graduate School of Biomedical Sciences, Dallas, TX, USA
Sci Rep 1:24. 2011..The lipidomes of cMGS and hMGS were found to be qualitatively similar, which implies similar biosynthetic and biodegradation pathways in canines and humans. However, some quantitative differences between the two were observed...- Toward an animal model of the human tear film: biochemical comparison of the mouse, canine, rabbit, and human meibomian lipidomesIgor A Butovich
Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
Invest Ophthalmol Vis Sci 53:6881-96. 2012.... 
Evaluation and quantitation of intact wax esters of human meibum by gas-liquid chromatography-ion trap mass spectrometryIgor A Butovich
Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9057, USA
Invest Ophthalmol Vis Sci 53:3766-81. 2012..Their complete characterization on the level of individual intact lipid species has not been completed yet. We obtained detailed structural information on previously uncharacterized meibomian WE...- Cholesteryl esters as a depot for very long chain fatty acids in human meibumIgor A Butovich
Department of Ophthalmology and Graduate School of Biomedical Sciences, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
J Lipid Res 50:501-13. 2009..Other Chl-containing compounds were detected but not identified at the time. Therefore, Chl-E are a depot for very long chain saturated and monounsaturated fatty acids in human meibum... - Meibomian lipid films and the impact of temperatureIgor A Butovich
Department of Ophthalmology, Graduate School of Biomedical Sciences, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9057, USA
Invest Ophthalmol Vis Sci 51:5508-18. 2010..This may have a detrimental impact on the stability and functionality of the human tear film and the tear film lipid layer. The goal of this project was to quantitatively examine the possible impact of temperature on the latter... - Human tear film and meibum. Very long chain wax esters and (O-acyl)-omega-hydroxy fatty acids of meibumIgor A Butovich
Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
J Lipid Res 50:2471-85. 2009..and the aqueous subphase beneath it, a role previously attributed to phospholipids... - On the lipid composition of human meibum and tears: comparative analysis of nonpolar lipidsIgor A Butovich
Department of Ophthalmology and Graduate School of Biomedical Sciences, University of Texas Southwestern Medical Center, Dallas, Texas, USA
Invest Ophthalmol Vis Sci 49:3779-89. 2008.... - Inhibition of lipoxygenases and cyclooxygenases by linoleyl hydroxamic acid: comparative in vitro studiesIgor A Butovich
Department of Ophthalmology and Graduate School of Biomedical Sciences, University of Texas Southwestern Medical Center, Dallas, TX, USA
J Lipid Res 49:1284-94. 2008..g., inflammation, cardiovascular disorders, cancer, asthma, allergies, psoriasis, and stroke... 
Lipids of human meibum: mass-spectrometric analysis and structural elucidationIgor A Butovich
Department of Ophthalmology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390 9057, USA
J Lipid Res 48:2220-35. 2007..These observations suggest that MGS are a major source of nonpolar lipids of the WE and Chl-E families for the tear film lipid layer, but not of its previously reported (phospho)lipid, Cer, and fatty acid amide components...- Liquid chromatography-mass spectrometric analysis of lipids present in human meibomian gland secretionsIgor A Butovich
Department of Ophthalmology, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, TX 75390 9057, USA
Lipids 42:765-76. 2007..These observations suggest that MGS are a major source of the nonpolar lipids of the WE and Chl-E families for the tear film lipid layer (TFLL), but not of the previously reported phospholipid components of the TFLL... - Lipidomics of human Meibomian gland secretions: Chemistry, biophysics, and physiological role of Meibomian lipidsIgor A Butovich
Department of Ophthalmology and the Graduate School of Biomedical Sciences, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390 9057, USA
Prog Lipid Res 50:278-301. 2011..It is concluded that despite a substantial progress in the area of Meibomian glands lipidomics, there are large areas of uncertainty that need to be addressed in future experiments... - Novel oxylipins formed from docosahexaenoic acid by potato lipoxygenase--10(S)-hydroxydocosahexaenoic acid and 10,20-dihydroxydocosahexaenoic acidIgor A Butovich
Division of Physiological Chemistry II, Department of Medical Biochemistry and Biophysics, Karolinska Institute, S 171 77, Stockholm, Sweden
Lipids 40:249-57. 2005..Our observation that ptLOX could convert 17-HDHA to 10,17-diH(P)DHA indicates that this dihydroxylated derivative of DHA also can be formed via a double lipoxygenation mechanism... - On the structure and synthesis of neuroprotectin D1, a novel anti-inflammatory compound of the docosahexaenoic acid familyIgor A Butovich
Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9057, USA
J Lipid Res 46:2311-4. 2005.... - A one-step method of 10,17-dihydro(pero)xydocosahexa-4Z,7Z,11E,13Z,15E,19Z-enoic acid synthesis by soybean lipoxygenaseIgor A Butovich
Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9057, USA
J Lipid Res 47:854-63. 2006..Compared with the previously described two-enzyme method that involved sLOX and ptLOX, the current simplified one-enzyme procedure uses only sLOX as the catalyst of both dioxygenation steps... - Dihydroxydocosahexaenoic acids of the neuroprotectin D family: synthesis, structure, and inhibition of human 5-lipoxygenaseIgor A Butovich
Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9057, USA
J Lipid Res 47:2462-74. 2006..These findings suggest that 7,17(S)-diHDHA along with its 10,17(S) counterpart might have anti-inflammatory and anticancer activities, which could be exerted, at least in part, through direct inhibition of 5LOX and 15LOX... - Epidermal expression of an Elovl4 transgene rescues neonatal lethality of homozygous Stargardt disease-3 miceAnne McMahon
Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
J Lipid Res 52:1128-38. 2011..In addition to the skin, Elovl4 is also expressed in other tissues, including the retina, brain, and testes. Thus, these mice will facilitate future studies to define the roles of C28-C36 fatty acids in the Elovl4-expressing tissues... - Impact on ocular surface evaporation of an artificial tear solution containing hydroxypropyl guarEduardo Uchiyama
Department of Ophthalmology, The University of Texas Southwestern Medical Center at Dallas, TX, USA
Eye Contact Lens 34:331-4. 2008.... - Disruption and destabilization of meibomian lipid films caused by increasing amounts of ceramides and cholesterolJuan C Arciniega
Department of Ophthalmology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9057, USA
Invest Ophthalmol Vis Sci 54:1352-60. 2013..We evaluated quantitatively direct effects of ceramide (Cer) and free cholesterol (FC) on meibomian lipid films (MLF) using a Langmuir trough (LT) and a Brewster angle microscope (BAM)... - Impact of evaporation on aqueous tear lossJames P McCulley
Department of Ophthalmology, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas, USA
Trans Am Ophthalmol Soc 104:121-8. 2006..The percent contribution is dependent on environmental conditions such as RH. There was a trend toward increased contribution to AT loss in dry eye patients vs controls, but statistical significance was not reached... - Increased evaporative rates in laboratory testing conditions simulating airplane cabin relative humidity: an important factor for dry eye syndromeEduardo Uchiyama
Department of Ophthalmology, The University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390 9057, USA
Eye Contact Lens 33:174-6. 2007.... - Pattern of vital staining and its correlation with aqueous tear deficiency and meibomian gland dropoutEduardo Uchiyama
Department of Ophthalmology, The University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390 9057, USA
Eye Contact Lens 33:177-9. 2007.... - Historical brief on composition of human meibum lipidsJadwiga C Wojtowicz
Department of Ophthalmology, The University of Texas Southwestern Medical Center at Dallas, Texas 75390 9057, USA
Ocul Surf 7:145-53. 2009..Historical analytical inconsistencies may be partly explained by limitations of past analytical procedures and by the consideration that the tear film lipid layer may have contributing sources other than meibum... - Effects of free fatty acids on meibomian lipid filmsJuan C Arciniega
Department of Ophthalmology, University of Texas Southwestern Medical Center at Dallas, TX 75390 9057, USA
Exp Eye Res 93:452-9. 2011.... - Understanding and analyzing meibomian lipids--a reviewIgor A Butovich
Department of Ophthalmology and Graduate School of Biomedical Sciences, University of Texas Southwestern Medical Center, Dallas, Texas, USA
Curr Eye Res 33:405-20. 2008..This review is intended to bring to the informed reader the current state of knowledge about meibomian lipids and the art for analyzing them... - Fatty acid composition of cholesteryl esters of human meibomian gland secretionsIgor A Butovich
Department of Ophthalmology and the Graduate School of Biomedical Sciences, University of Texas Southwestern Medical Center, Dallas, TX, USA
Steroids 75:726-33. 2010..We conclude that the FA composition of CE can be successfully established in LC-MS experiments conducted in NIM. Meibomian CE have a large presence of both saturated and unsaturated FA with an average molar ratio of 4 to 1, respectively... - The Meibomian puzzle: combining pieces togetherIgor A Butovich
Department of Ophthalmology and Graduate School of Biomedical Sciences, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390 9057, USA
Prog Retin Eye Res 28:483-98. 2009..Thus, these compounds may become an important target in biochemistry and (patho)physiology of ocular surface and dry eye research... - The spectrophotometric sulfo-phospho-vanillin assessment of total lipids in human meibomian gland secretionsAnne McMahon
Department of Ophthalmology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX, 75390 9057, USA
Lipids 48:513-25. 2013..The SPV method can also be adopted for analyzing other types of complex lipids secretions, such as sebum, as well as whole lipid extracts from other lipid-enriched organs and tissues, if proper standards are chosen... - Retinal pathology and skin barrier defect in mice carrying a Stargardt disease-3 mutation in elongase of very long chain fatty acids-4Anne McMahon
Department of Ophthalmology, The University of Texas Southwestern Medical Center, Dallas, TX 75390 9057, USA
Mol Vis 13:258-72. 2007..The goal of this study was to generate and characterize heterozygous and homozygous knockin-mice that carry a human STGD3 pathogenic mutation in the mouse Elovl4 gene... - Lipidomic analysis of human meibum using HPLC-MSnIgor A Butovich
Department of Ophthalmology, University of Texas, Southwestern Medical Center, Dallas, TX, USA
Methods Mol Biol 579:221-46. 2009.... - Correlations in a change in aqueous tear evaporation with a change in relative humidity and the impactJames P McCulley
Department of Ophthalmology, The University of Texas Southwestern Medical Center, Dallas, Texas 75390 9057, USA
Am J Ophthalmol 141:758-60. 2006..To establish scientific relationship between relative humidity (RH) and aqueous tear evaporation to elucidate possible significance of this relationship in normals and aqueous tear deficiency patients... - Inhibition of potato lipoxygenase by linoleyl hydroxamic acid: kinetic and EPR spectral evidence for a two-step reactionIgor A Butovich
Department of Pharmaceutical Sciences, College of Pharmacy, Medical University of South Carolina, Charleston, SC 29425, U S A
Biochem J 365:865-71. 2002..35 signal. The quenching is attributed to the reduction of Fe(3+)-ptLOX by LHA, with concomitant formation of its oxidation product(s). A kinetic scheme for the inhibition is proposed... - The biosynthetic gene cluster for the antitumor rebeccamycin: characterization and generation of indolocarbazole derivativesCesar Sanchez
Departamento de Biología Funcional e Instituto, Universitario de Oncología del Principado de Asturias, Universidad de Oviedo, 33006 Oviedo, Spain
Chem Biol 9:519-31. 2002..The cloned genes should help to elucidate the molecular basis for indolocarbazole biosynthesis and set the stage for the generation of novel indolocarbazole analogues by genetic engineering...
Research Grants
- Biochemistry and Biophysics of the Preocular Tear FilmIgor A Butovich; Fiscal Year: 2010....
- Biochemistry and Biophysics of the Preocular Tear FilmIgor A Butovich; Fiscal Year: 2010....