Igor A Butovich

Summary

Affiliation: University of Texas Southwestern Medical Center
Country: USA

Publications

  1. pmc Tear film lipids
    Igor A Butovich
    Department of Ophthalmology and the Graduate School of Biomedical Sciences, University of Texas Southwestern Medical Center, Dallas, TX 75390 9057, USA Electronic address
    Exp Eye Res 117:4-27. 2013
  2. pmc Comparative HPLC-MS analysis of canine and human meibomian lipidomes: many similarities, a few differences
    Igor A Butovich
    University of Texas Southwestern Medical Center, Department of Ophthalmology and the Graduate School of Biomedical Sciences, Dallas, TX, USA
    Sci Rep 1:24. 2011
  3. pmc Toward an animal model of the human tear film: biochemical comparison of the mouse, canine, rabbit, and human meibomian lipidomes
    Igor A Butovich
    Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Invest Ophthalmol Vis Sci 53:6881-96. 2012
  4. doi request reprint Evaluation and quantitation of intact wax esters of human meibum by gas-liquid chromatography-ion trap mass spectrometry
    Igor A Butovich
    Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9057, USA
    Invest Ophthalmol Vis Sci 53:3766-81. 2012
  5. pmc Lipidomics of human Meibomian gland secretions: Chemistry, biophysics, and physiological role of Meibomian lipids
    Igor A Butovich
    Department of Ophthalmology and the Graduate School of Biomedical Sciences, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390 9057, USA
    Prog Lipid Res 50:278-301. 2011
  6. pmc Inhibition of lipoxygenases and cyclooxygenases by linoleyl hydroxamic acid: comparative in vitro studies
    Igor A Butovich
    Department of Ophthalmology and Graduate School of Biomedical Sciences, University of Texas Southwestern Medical Center, Dallas, TX, USA
    J Lipid Res 49:1284-94. 2008
  7. pmc On the lipid composition of human meibum and tears: comparative analysis of nonpolar lipids
    Igor A Butovich
    Department of Ophthalmology and Graduate School of Biomedical Sciences, University of Texas Southwestern Medical Center, Dallas, Texas, USA
    Invest Ophthalmol Vis Sci 49:3779-89. 2008
  8. pmc Cholesteryl esters as a depot for very long chain fatty acids in human meibum
    Igor A Butovich
    Department of Ophthalmology and Graduate School of Biomedical Sciences, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    J Lipid Res 50:501-13. 2009
  9. pmc Meibomian lipid films and the impact of temperature
    Igor A Butovich
    Department of Ophthalmology, Graduate School of Biomedical Sciences, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9057, USA
    Invest Ophthalmol Vis Sci 51:5508-18. 2010
  10. pmc Human tear film and meibum. Very long chain wax esters and (O-acyl)-omega-hydroxy fatty acids of meibum
    Igor A Butovich
    Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    J Lipid Res 50:2471-85. 2009

Research Grants

Collaborators

Detail Information

Publications35

  1. pmc Tear film lipids
    Igor A Butovich
    Department of Ophthalmology and the Graduate School of Biomedical Sciences, University of Texas Southwestern Medical Center, Dallas, TX 75390 9057, USA Electronic address
    Exp Eye Res 117:4-27. 2013
    ..A map of the meibomian lipidome (as it is seen in reverse phase liquid chromatography/mass spectrometry experiments) is presented. Directions of future efforts in the area are outlined. ..
  2. pmc Comparative HPLC-MS analysis of canine and human meibomian lipidomes: many similarities, a few differences
    Igor A Butovich
    University of Texas Southwestern Medical Center, Department of Ophthalmology and the Graduate School of Biomedical Sciences, Dallas, TX, USA
    Sci Rep 1:24. 2011
    ..The lipidomes of cMGS and hMGS were found to be qualitatively similar, which implies similar biosynthetic and biodegradation pathways in canines and humans. However, some quantitative differences between the two were observed...
  3. pmc Toward an animal model of the human tear film: biochemical comparison of the mouse, canine, rabbit, and human meibomian lipidomes
    Igor A Butovich
    Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Invest Ophthalmol Vis Sci 53:6881-96. 2012
    ....
  4. doi request reprint Evaluation and quantitation of intact wax esters of human meibum by gas-liquid chromatography-ion trap mass spectrometry
    Igor A Butovich
    Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9057, USA
    Invest Ophthalmol Vis Sci 53:3766-81. 2012
    ..Their complete characterization on the level of individual intact lipid species has not been completed yet. We obtained detailed structural information on previously uncharacterized meibomian WE...
  5. pmc Lipidomics of human Meibomian gland secretions: Chemistry, biophysics, and physiological role of Meibomian lipids
    Igor A Butovich
    Department of Ophthalmology and the Graduate School of Biomedical Sciences, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390 9057, USA
    Prog Lipid Res 50:278-301. 2011
    ..It is concluded that despite a substantial progress in the area of Meibomian glands lipidomics, there are large areas of uncertainty that need to be addressed in future experiments...
  6. pmc Inhibition of lipoxygenases and cyclooxygenases by linoleyl hydroxamic acid: comparative in vitro studies
    Igor A Butovich
    Department of Ophthalmology and Graduate School of Biomedical Sciences, University of Texas Southwestern Medical Center, Dallas, TX, USA
    J Lipid Res 49:1284-94. 2008
    ..g., inflammation, cardiovascular disorders, cancer, asthma, allergies, psoriasis, and stroke...
  7. pmc On the lipid composition of human meibum and tears: comparative analysis of nonpolar lipids
    Igor A Butovich
    Department of Ophthalmology and Graduate School of Biomedical Sciences, University of Texas Southwestern Medical Center, Dallas, Texas, USA
    Invest Ophthalmol Vis Sci 49:3779-89. 2008
    ....
  8. pmc Cholesteryl esters as a depot for very long chain fatty acids in human meibum
    Igor A Butovich
    Department of Ophthalmology and Graduate School of Biomedical Sciences, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    J Lipid Res 50:501-13. 2009
    ..Other Chl-containing compounds were detected but not identified at the time. Therefore, Chl-E are a depot for very long chain saturated and monounsaturated fatty acids in human meibum...
  9. pmc Meibomian lipid films and the impact of temperature
    Igor A Butovich
    Department of Ophthalmology, Graduate School of Biomedical Sciences, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9057, USA
    Invest Ophthalmol Vis Sci 51:5508-18. 2010
    ..This may have a detrimental impact on the stability and functionality of the human tear film and the tear film lipid layer. The goal of this project was to quantitatively examine the possible impact of temperature on the latter...
  10. pmc Human tear film and meibum. Very long chain wax esters and (O-acyl)-omega-hydroxy fatty acids of meibum
    Igor A Butovich
    Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    J Lipid Res 50:2471-85. 2009
    ..and the aqueous subphase beneath it, a role previously attributed to phospholipids...
  11. ncbi request reprint Lipids of human meibum: mass-spectrometric analysis and structural elucidation
    Igor A Butovich
    Department of Ophthalmology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390 9057, USA
    J Lipid Res 48:2220-35. 2007
    ..These observations suggest that MGS are a major source of nonpolar lipids of the WE and Chl-E families for the tear film lipid layer, but not of its previously reported (phospho)lipid, Cer, and fatty acid amide components...
  12. ncbi request reprint Liquid chromatography-mass spectrometric analysis of lipids present in human meibomian gland secretions
    Igor A Butovich
    Department of Ophthalmology, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, TX 75390 9057, USA
    Lipids 42:765-76. 2007
    ..These observations suggest that MGS are a major source of the nonpolar lipids of the WE and Chl-E families for the tear film lipid layer (TFLL), but not of the previously reported phospholipid components of the TFLL...
  13. ncbi request reprint Dihydroxydocosahexaenoic acids of the neuroprotectin D family: synthesis, structure, and inhibition of human 5-lipoxygenase
    Igor A Butovich
    Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9057, USA
    J Lipid Res 47:2462-74. 2006
    ..These findings suggest that 7,17(S)-diHDHA along with its 10,17(S) counterpart might have anti-inflammatory and anticancer activities, which could be exerted, at least in part, through direct inhibition of 5LOX and 15LOX...
  14. ncbi request reprint A one-step method of 10,17-dihydro(pero)xydocosahexa-4Z,7Z,11E,13Z,15E,19Z-enoic acid synthesis by soybean lipoxygenase
    Igor A Butovich
    Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9057, USA
    J Lipid Res 47:854-63. 2006
    ..Compared with the previously described two-enzyme method that involved sLOX and ptLOX, the current simplified one-enzyme procedure uses only sLOX as the catalyst of both dioxygenation steps...
  15. ncbi request reprint On the structure and synthesis of neuroprotectin D1, a novel anti-inflammatory compound of the docosahexaenoic acid family
    Igor A Butovich
    Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9057, USA
    J Lipid Res 46:2311-4. 2005
    ....
  16. ncbi request reprint Novel oxylipins formed from docosahexaenoic acid by potato lipoxygenase--10(S)-hydroxydocosahexaenoic acid and 10,20-dihydroxydocosahexaenoic acid
    Igor A Butovich
    Division of Physiological Chemistry II, Department of Medical Biochemistry and Biophysics, Karolinska Institute, S 171 77, Stockholm, Sweden
    Lipids 40:249-57. 2005
    ..Our observation that ptLOX could convert 17-HDHA to 10,17-diH(P)DHA indicates that this dihydroxylated derivative of DHA also can be formed via a double lipoxygenation mechanism...
  17. pmc Epidermal expression of an Elovl4 transgene rescues neonatal lethality of homozygous Stargardt disease-3 mice
    Anne McMahon
    Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    J Lipid Res 52:1128-38. 2011
    ..In addition to the skin, Elovl4 is also expressed in other tissues, including the retina, brain, and testes. Thus, these mice will facilitate future studies to define the roles of C28-C36 fatty acids in the Elovl4-expressing tissues...
  18. pmc The spectrophotometric sulfo-phospho-vanillin assessment of total lipids in human meibomian gland secretions
    Anne McMahon
    Department of Ophthalmology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390 9057, USA
    Lipids 48:513-25. 2013
    ..The SPV method can also be adopted for analyzing other types of complex lipids secretions, such as sebum, as well as whole lipid extracts from other lipid-enriched organs and tissues, if proper standards are chosen...
  19. pmc Impact on ocular surface evaporation of an artificial tear solution containing hydroxypropyl guar
    Eduardo Uchiyama
    Department of Ophthalmology, The University of Texas Southwestern Medical Center at Dallas, TX, USA
    Eye Contact Lens 34:331-4. 2008
    ....
  20. pmc Biophysical and morphological evaluation of human normal and dry eye meibum using hot stage polarized light microscopy
    Igor A Butovich
    Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas, Texas
    Invest Ophthalmol Vis Sci 55:87-101. 2014
    ..To study melting characteristics and the morphology of human and mouse meibum...
  21. pmc Disruption and destabilization of meibomian lipid films caused by increasing amounts of ceramides and cholesterol
    Juan C Arciniega
    Department of Ophthalmology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9057, USA
    Invest Ophthalmol Vis Sci 54:1352-60. 2013
    ..We evaluated quantitatively direct effects of ceramide (Cer) and free cholesterol (FC) on meibomian lipid films (MLF) using a Langmuir trough (LT) and a Brewster angle microscope (BAM)...
  22. ncbi request reprint Increased evaporative rates in laboratory testing conditions simulating airplane cabin relative humidity: an important factor for dry eye syndrome
    Eduardo Uchiyama
    Department of Ophthalmology, The University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390 9057, USA
    Eye Contact Lens 33:174-6. 2007
    ....
  23. pmc Impact of evaporation on aqueous tear loss
    James P McCulley
    Department of Ophthalmology, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas, USA
    Trans Am Ophthalmol Soc 104:121-8. 2006
    ..To determine the impact of evaporation on preocular aqueous tear (AT) loss in normal subjects (controls) and patients with keratoconjunctivitis sicca (KCS)...
  24. ncbi request reprint Pattern of vital staining and its correlation with aqueous tear deficiency and meibomian gland dropout
    Eduardo Uchiyama
    Department of Ophthalmology, The University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390 9057, USA
    Eye Contact Lens 33:177-9. 2007
    ....
  25. ncbi request reprint Historical brief on composition of human meibum lipids
    Jadwiga C Wojtowicz
    Department of Ophthalmology, The University of Texas Southwestern Medical Center at Dallas, Texas 75390 9057, USA
    Ocul Surf 7:145-53. 2009
    ..Historical analytical inconsistencies may be partly explained by limitations of past analytical procedures and by the consideration that the tear film lipid layer may have contributing sources other than meibum...
  26. pmc Effects of free fatty acids on meibomian lipid films
    Juan C Arciniega
    Department of Ophthalmology, University of Texas Southwestern Medical Center at Dallas, TX 75390 9057, USA
    Exp Eye Res 93:452-9. 2011
    ....
  27. pmc Understanding and analyzing meibomian lipids--a review
    Igor A Butovich
    Department of Ophthalmology and Graduate School of Biomedical Sciences, University of Texas Southwestern Medical Center, Dallas, Texas, USA
    Curr Eye Res 33:405-20. 2008
    ..This review is intended to bring to the informed reader the current state of knowledge about meibomian lipids and the art for analyzing them...
  28. pmc Differential scanning calorimetric evaluation of human meibomian gland secretions and model lipid mixtures: transition temperatures and cooperativity of melting
    Hua Lu
    Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9057, United States
    Chem Phys Lipids 170:55-64. 2013
    ..The highly asymmetrical shape of the thermotropic peak of meibum is important for the physiology and biophysics of TFLL...
  29. pmc Fatty acid composition of cholesteryl esters of human meibomian gland secretions
    Igor A Butovich
    Department of Ophthalmology and the Graduate School of Biomedical Sciences, University of Texas Southwestern Medical Center, Dallas, TX, USA
    Steroids 75:726-33. 2010
    ..We conclude that the FA composition of CE can be successfully established in LC-MS experiments conducted in NIM. Meibomian CE have a large presence of both saturated and unsaturated FA with an average molar ratio of 4 to 1, respectively...
  30. pmc The Meibomian puzzle: combining pieces together
    Igor A Butovich
    Department of Ophthalmology and Graduate School of Biomedical Sciences, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390 9057, USA
    Prog Retin Eye Res 28:483-98. 2009
    ..Thus, these compounds may become an important target in biochemistry and (patho)physiology of ocular surface and dry eye research...
  31. pmc Retinal pathology and skin barrier defect in mice carrying a Stargardt disease-3 mutation in elongase of very long chain fatty acids-4
    Anne McMahon
    Department of Ophthalmology, The University of Texas Southwestern Medical Center, Dallas, TX 75390 9057, USA
    Mol Vis 13:258-72. 2007
    ..The goal of this study was to generate and characterize heterozygous and homozygous knockin-mice that carry a human STGD3 pathogenic mutation in the mouse Elovl4 gene...
  32. doi request reprint Lipidomic analysis of human meibum using HPLC-MSn
    Igor A Butovich
    Department of Ophthalmology, University of Texas, Southwestern Medical Center, Dallas, TX, USA
    Methods Mol Biol 579:221-46. 2009
    ....
  33. ncbi request reprint Correlations in a change in aqueous tear evaporation with a change in relative humidity and the impact
    James P McCulley
    Department of Ophthalmology, The University of Texas Southwestern Medical Center, Dallas, Texas 75390 9057, USA
    Am J Ophthalmol 141:758-60. 2006
    ..To establish scientific relationship between relative humidity (RH) and aqueous tear evaporation to elucidate possible significance of this relationship in normals and aqueous tear deficiency patients...
  34. pmc Inhibition of potato lipoxygenase by linoleyl hydroxamic acid: kinetic and EPR spectral evidence for a two-step reaction
    Igor A Butovich
    Department of Pharmaceutical Sciences, College of Pharmacy, Medical University of South Carolina, Charleston, SC 29425, U S A
    Biochem J 365:865-71. 2002
    ..35 signal. The quenching is attributed to the reduction of Fe(3+)-ptLOX by LHA, with concomitant formation of its oxidation product(s). A kinetic scheme for the inhibition is proposed...
  35. ncbi request reprint The biosynthetic gene cluster for the antitumor rebeccamycin: characterization and generation of indolocarbazole derivatives
    Cesar Sanchez
    Departamento de BiologĂ­a Funcional e Instituto, Universitario de OncologĂ­a del Principado de Asturias, Universidad de Oviedo, 33006 Oviedo, Spain
    Chem Biol 9:519-31. 2002
    ..The cloned genes should help to elucidate the molecular basis for indolocarbazole biosynthesis and set the stage for the generation of novel indolocarbazole analogues by genetic engineering...

Research Grants2

  1. Biochemistry and Biophysics of the Preocular Tear Film
    Igor A Butovich; Fiscal Year: 2010
    ....
  2. Biochemistry and Biophysics of the Preocular Tear Film
    Igor A Butovich; Fiscal Year: 2010
    ....