Affiliation: University of Oklahoma Health Sciences Center
- Real-time measurement of in vitro peptide binding to soluble HLA-A*0201 by fluorescence polarizationRico Buchli
Pure Protein L L C, Oklahoma City, Oklahoma 73104 3698, USA
Biochemistry 43:14852-63. 2004..This assay therefore represents a versatile tool for characterizing the binding of peptide epitopes during the development of class I HLA-based vaccines and immune therapies...
- Identification of breast cancer peptide epitopes presented by HLA-A*0201Oriana E Hawkins
University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, Pure Protein, LLC, Oklahoma City, Oklahoma 73104, USA
J Proteome Res 7:1445-57. 2008..The identification and characterization of peptides unique to the class I of breast cancer cells provide putative targets for the development of immune diagnostic tools and therapeutics...
- Development and validation of a fluorescence polarization-based competitive peptide-binding assay for HLA-A*0201--a new tool for epitope discoveryRico Buchli
Pure Protein L L C, Oklahoma City, Oklahoma 73104 3698, USA
Biochemistry 44:12491-507. 2005..Furthermore, the assay also features high-throughput screening capacity, providing uniquely efficient means of identifying and evaluating immune target molecules...
- Critical factors in the development of fluorescence polarization-based peptide binding assays: an equilibrium study monitoring specific peptide binding to soluble HLA-A*0201Rico Buchli
Pure Protein L L C, Oklahoma City, OK 73104 3698, United States
J Immunol Methods 314:38-53. 2006..With the need for both increased throughput and miniaturized volumes, this fully homogenous, fluorescent-type binding assay is expected to be useful for routine analysis of peptide binding to MHC class I as well as class II molecules...
- HLA class I molecules consistently present internal influenza epitopesAngela Wahl
Department of Microbiology and Immunology, Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, 975 NE 10th Street, Oklahoma City, OK 73104, USA
Proc Natl Acad Sci U S A 106:540-5. 2009..These data demonstrate an emerging pattern whereby class I HLA reveal a handful of internal viral ligands and whereby CTL recognize consistently presented influenza ligands...
- T-cell tolerance for variability in an HLA class I-presented influenza A virus epitopeAngela Wahl
Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, 73104, USA
J Virol 83:9206-14. 2009..These data demonstrate that substitutions at P4 and/or P6 facilitate influenza virus escape from T-cell recognition and provide a model for the number, nature, and location of viral mutations that influence T-cell cross-recognition...
- Toward a definition of self: proteomic evaluation of the class I peptide repertoireHeather D Hickman
Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
J Immunol 172:2944-52. 2004..Importantly, virus- and tumor-induced changes in virtually any cellular compartment or to any chromosome can be expected to be presented by class I molecules for immune recognition...
- Cutting edge: class I presentation of host peptides following HIV infectionHeather D Hickman
Department of Microbiology and Immunology and William K Warren Medical Research Institute, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
J Immunol 171:22-6. 2003..Therefore, comprehensive analysis of the B*0702 peptide repertoire demonstrates that marked differences in host protein presentation occur after HIV infection...
- Epitope discovery in West Nile virus infection: Identification and immune recognition of viral epitopesCurtis P McMurtrey
Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, 975 NE 10th Street, Oklahoma City, OK 73104, USA
Proc Natl Acad Sci U S A 105:2981-6. 2008..Detection and recovery from WNV infection are therefore functions of the ability of class I HLA molecules to reveal conserved WNV epitopes to an intact cellular immune system that subsequently recognizes infected cells...
- Motif inference reveals optimal CTL epitopes presented by HLA class I alleles highly prevalent in southern AfricaIsobella Honeyborne
Department of Paediatrics, Nuffield Department of Medicine, Peter Medawar Building for Pathogen Research, Oxford, United Kingdom
J Immunol 176:4699-705. 2006..These data indicate that the previously anomalous position of HLA-A*2902 among HLA-A alleles, outside any recognized HLA-A supertype, is artifactual, and the true position of the A*2902 motif overlaps those of the A1 and A24 supertypes...
- MHC class I-associated peptides identified from normal platelets and from individuals with idiopathic thrombocytopenic purpuraLeann M Hopkins
Department of Pathobiology and Diagnostic Investigation, Michigan State University, East Lansing, MI, USA
Hum Immunol 66:874-83. 2005..In conclusion, platelets from normal and ITP individuals can present peptides from general cellular proteins and platelet specific proteins, such as GPIb, to the immune system via MHC class I...
- Assessing vaccine potency using TCRmimic antibodiesFrancisca A Neethling
Receptor Logic Ltd, Abilene, TX 79601, United States
Vaccine 26:3092-102. 2008..TCRm staining of vaccine-treated mDCs showed detection of the TMT and GVL peptide-HLA-A2 complexes. These findings demonstrate that TCRms may be important tools for determining the potency of DC-based vaccines...
- Processing and presentation of exogenous HLA class I peptides by dendritic cells from human immunodeficiency virus type 1-infected personsXiao li Huang
University of Pittsburgh Graduate School of Public Health, 130 DeSoto St, Pittsburgh, PA 15261, USA
J Virol 79:3052-62. 2005..This conclusion supports the use of DCs loaded with synthetic peptides in immunotherapy of HIV-1 infection...