JEFFREY BRODSKY

Summary

Affiliation: University of Pittsburgh
Country: USA

Publications

  1. pmc Protein folding and quality control in the endoplasmic reticulum: Recent lessons from yeast and mammalian cell systems
    Jeffrey L Brodsky
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Curr Opin Cell Biol 23:464-75. 2011
  2. pmc Expression of a malarial Hsp70 improves defects in chaperone-dependent activities in ssa1 mutant yeast
    Samantha L Bell
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 6:e20047. 2011
  3. pmc The threads that tie protein-folding diseases
    Jeffrey L Brodsky
    Department of Biological Sciences, A320 Langley Hall, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Dis Model Mech 7:3-4. 2014
  4. doi request reprint Just a trim, please: refining ER degradation through deubiquitination
    Jeffrey L Brodsky
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Cell 154:479-81. 2013
  5. pmc Cleaning up: ER-associated degradation to the rescue
    Jeffrey L Brodsky
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Cell 151:1163-7. 2012
  6. ncbi request reprint Hsp70 molecular chaperones: emerging roles in human disease and identification of small molecule modulators
    Jeffrey L Brodsky
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260 USA
    Curr Top Med Chem 6:1215-25. 2006
  7. pmc Substrate-specific mediators of ER associated degradation (ERAD)
    Jeffrey L Brodsky
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Curr Opin Cell Biol 21:516-21. 2009
  8. pmc The protective and destructive roles played by molecular chaperones during ERAD (endoplasmic-reticulum-associated degradation)
    Jeffrey L Brodsky
    Department of Biological Sciences, 274A Crawford Hall, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Biochem J 404:353-63. 2007
  9. pmc The many intersecting pathways underlying apolipoprotein B secretion and degradation
    Jeffrey L Brodsky
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Trends Endocrinol Metab 19:254-9. 2008
  10. ncbi request reprint An in vitro assay for the selective endoplasmic reticulum associated degradation of an unglycosylated secreted protein
    Jeffrey L Brodsky
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Methods 35:354-9. 2005

Collaborators

Detail Information

Publications61

  1. pmc Protein folding and quality control in the endoplasmic reticulum: Recent lessons from yeast and mammalian cell systems
    Jeffrey L Brodsky
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Curr Opin Cell Biol 23:464-75. 2011
    ..Not surprisingly, a growing number of human diseases are linked to various facets of ER function. Each of these topics will be discussed in this article, with an emphasis on recent reports in the literature that employed diverse models...
  2. pmc Expression of a malarial Hsp70 improves defects in chaperone-dependent activities in ssa1 mutant yeast
    Samantha L Bell
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 6:e20047. 2011
    ..Together, we conclude that yeast can be co-opted to rapidly uncover specific cellular activities mediated by malarial chaperones...
  3. pmc The threads that tie protein-folding diseases
    Jeffrey L Brodsky
    Department of Biological Sciences, A320 Langley Hall, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Dis Model Mech 7:3-4. 2014
    ....
  4. doi request reprint Just a trim, please: refining ER degradation through deubiquitination
    Jeffrey L Brodsky
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Cell 154:479-81. 2013
    ..Only a minor increase in the interaction between a protein and a ubiquitin ligase is sufficient to signal substrate degradation. Zhang et al. have identified deubiquitination as a signal amplifier. ..
  5. pmc Cleaning up: ER-associated degradation to the rescue
    Jeffrey L Brodsky
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Cell 151:1163-7. 2012
    ..This process is termed endoplasmic-reticulum (ER)-associated degradation (ERAD)...
  6. ncbi request reprint Hsp70 molecular chaperones: emerging roles in human disease and identification of small molecule modulators
    Jeffrey L Brodsky
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260 USA
    Curr Top Med Chem 6:1215-25. 2006
    ..Finally, we will review the recent discovery of small molecules that alter Hsp70 expression and function...
  7. pmc Substrate-specific mediators of ER associated degradation (ERAD)
    Jeffrey L Brodsky
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Curr Opin Cell Biol 21:516-21. 2009
    ..Other substrates fold and function normally but can be selected for ERAD by protein adaptors that identify and transmit regulatory cues...
  8. pmc The protective and destructive roles played by molecular chaperones during ERAD (endoplasmic-reticulum-associated degradation)
    Jeffrey L Brodsky
    Department of Biological Sciences, 274A Crawford Hall, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Biochem J 404:353-63. 2007
    ....
  9. pmc The many intersecting pathways underlying apolipoprotein B secretion and degradation
    Jeffrey L Brodsky
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Trends Endocrinol Metab 19:254-9. 2008
    ....
  10. ncbi request reprint An in vitro assay for the selective endoplasmic reticulum associated degradation of an unglycosylated secreted protein
    Jeffrey L Brodsky
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Methods 35:354-9. 2005
    ..The purpose of this chapter is first to discuss the experimental findings from the use of the in vitro assay, and then to describe the assay in detail. Finally, future potential uses of the in vitro system are illustrated...
  11. pmc The recognition and retrotranslocation of misfolded proteins from the endoplasmic reticulum
    Kunio Nakatsukasa
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Traffic 9:861-70. 2008
    ....
  12. pmc One step at a time: endoplasmic reticulum-associated degradation
    Shruthi S Vembar
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    Nat Rev Mol Cell Biol 9:944-57. 2008
    ..Here, we summarize our current understanding of each step during ERAD, with emphasis on the factors that catalyse distinct activities...
  13. pmc The Hsp110 molecular chaperone stabilizes apolipoprotein B from endoplasmic reticulum-associated degradation (ERAD)
    Stacy L Hrizo
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    J Biol Chem 282:32665-75. 2007
    ..This study indicates that chaperones within distinct complexes can play unique roles during ER-associated degradation (ERAD), establishes a role for Sse1/Hsp110 in ERAD, and identifies Hsp110 as a target to lower cholesterol...
  14. ncbi request reprint Degradation of mutated bovine pancreatic trypsin inhibitor in the yeast vacuole suggests post-endoplasmic reticulum protein quality control
    Christina M Coughlan
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    J Biol Chem 279:15289-97. 2004
    ..Because only the mutant protein is quantitatively proteolyzed these data suggest that a post-ER quality control check-point diverts unstable proteins to the vacuole for degradation...
  15. pmc J domain co-chaperone specificity defines the role of BiP during protein translocation
    Shruthi S Vembar
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    J Biol Chem 285:22484-94. 2010
    ..Together, our results indicate that residues within the Hsp70 J domain-interacting surface help confer Hsp40 specificity, in turn influencing distinct chaperone-mediated cellular activities...
  16. ncbi request reprint Cysteine string protein monitors late steps in cystic fibrosis transmembrane conductance regulator biogenesis
    Hui Zhang
    Department of Cell Biology and Physiology, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 281:11312-21. 2006
    ....
  17. ncbi request reprint Checkpoints in ER-associated degradation: excuse me, which way to the proteasome?
    Annette Ahner
    Department of Biological Sciences, University of Pittsburgh, 274 Crawford Hall, 4249 Fifth Avenue, Pittsburgh, PA 15260, USA
    Trends Cell Biol 14:474-8. 2004
    ..Two recent studies indicate that ERAD substrates are targeted to different pathways depending on the topology of the substrate and the subcellular location of the misfolded domain...
  18. pmc Dissecting the ER-associated degradation of a misfolded polytopic membrane protein
    Kunio Nakatsukasa
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Cell 132:101-12. 2008
    ..These data indicate that polytopic membrane proteins can be extracted from the ER, and define the point of action of chaperones and the requirement for Ufd2p during membrane protein quality control...
  19. pmc The activities and function of molecular chaperones in the endoplasmic reticulum
    Teresa M Buck
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, United States
    Semin Cell Dev Biol 18:751-61. 2007
    ..Nevertheless, we are only beginning to understand how chaperones function, how they are recruited to specific substrates and assist in folding/degradation, and how unique chaperone classes make quality control "decisions"...
  20. pmc ADD66, a gene involved in the endoplasmic reticulum-associated degradation of alpha-1-antitrypsin-Z in yeast, facilitates proteasome activity and assembly
    Craig M Scott
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Mol Biol Cell 18:3776-87. 2007
    ..These data identify Add66p as a proteasome assembly chaperone (PAC), and they provide the first link between PAC activity and ERAD...
  21. ncbi request reprint Small molecule modulators of endogenous and co-chaperone-stimulated Hsp70 ATPase activity
    Sheara W Fewell
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    J Biol Chem 279:51131-40. 2004
    ..Together, these data indicate the potential for continued screening of small molecule Hsp70 effectors and that specific modulators of Hsp70-Hsp40 interaction can be obtained, potentially for future therapeutic use...
  22. pmc Nucleotide exchange factor for the yeast Hsp70 molecular chaperone Ssa1p
    Mehdi Kabani
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    Mol Cell Biol 22:4677-89. 2002
    ..In support of this hypothesis, Fes1p was found to be associated with ribosomes...
  23. pmc Uncoupling retro-translocation and degradation in the ER-associated degradation of a soluble protein
    Robert J Lee
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    EMBO J 23:2206-15. 2004
    ..Moreover, addition of only the 19S cap of the proteasome catalyzed ATP-dependent export of the polypeptide substrate, which was degraded upon subsequent addition of the 20S particle...
  24. pmc Tipping the delicate balance: defining how proteasome maturation affects the degradation of a substrate for autophagy and endoplasmic reticulum associated degradation (ERAD)
    Jeffrey L Brodsky
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    Autophagy 3:623-5. 2007
    ..Because PACs are conserved, it will be critical to analyze whether these dedicated chaperones are implicated in other diseases associated with ERAD and autophagy...
  25. pmc Overexpression of yeast Hsp110 homolog Sse1p suppresses ydj1-151 thermosensitivity and restores Hsp90-dependent activity
    Jennifer L Goeckeler
    Department of Biological Sciences, University of Pittsburgh, Pennsylvania 15260, USA
    Mol Biol Cell 13:2760-70. 2002
    ..Because the folding of both v-Src kinase and human androgen receptor in yeast requires the Hsp90 complex, these data suggest that Ydj1p and Sse1p are interacting cochaperones in the Hsp90 complex and facilitate Hsp90-dependent activity...
  26. ncbi request reprint Reconstitution of endoplasmic reticulum-associated degradation using yeast membranes and cytosol
    Robert J Lee
    Department of Biological Sciences, University of Pittsburgh, PA, USA
    Methods Mol Biol 301:175-84. 2005
    ..This chapter describes this in vitro ERAD assay in detail, and special considerations when performing the assay are noted...
  27. pmc in vitro reconstitution of the selection, ubiquitination, and membrane extraction of a polytopic ERAD substrate
    Kunio Nakatsukasa
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA, USA
    Methods Mol Biol 619:365-76. 2010
    ..These techniques provide a powerful tool to further dissect the ERAD pathway into elementary steps...
  28. pmc Dependence of endoplasmic reticulum-associated degradation on the peptide binding domain and concentration of BiP
    Mehdi Kabani
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    Mol Biol Cell 14:3437-48. 2003
    ....
  29. pmc Distinct roles for the Hsp40 and Hsp90 molecular chaperones during cystic fibrosis transmembrane conductance regulator degradation in yeast
    Robert T Youker
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Mol Biol Cell 15:4787-97. 2004
    ..These results indicate that Hsp90, but not the Hsp90 complex, maintains CFTR structural integrity, whereas Ydj1p/Hlj1p catalyze CFTR degradation...
  30. pmc Small heat shock protein alphaA-crystallin regulates epithelial sodium channel expression
    Ossama B Kashlan
    Renal Electrolyte Division, Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 282:28149-56. 2007
    ..These data are consistent with the hypothesis that small heat shock proteins recognize ENaC subunits at ER quality control checkpoints and can target ENaC subunits for ER-associated degradation...
  31. ncbi request reprint Localization of the BiP molecular chaperone with respect to endoplasmic reticulum foci containing the cystic fibrosis transmembrane conductance regulator in yeast
    Mara L Sullivan
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    J Histochem Cytochem 51:545-8. 2003
    ..We propose that these sites are Russell bodies, an ER subcompartment in which misfolded proteins are stored and can be targeted for degradation...
  32. ncbi request reprint Vesicular trafficking of hepatic apolipoprotein B100 and its maturation to very low-density lipoprotein particles; studies from cells and cell-free systems
    Jeffrey L Brodsky
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Trends Cardiovasc Med 14:127-32. 2004
    ....
  33. pmc Small heat-shock proteins select deltaF508-CFTR for endoplasmic reticulum-associated degradation
    Annette Ahner
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Mol Biol Cell 18:806-14. 2007
    ....
  34. pmc The mammalian Hsp40 ERdj3 requires its Hsp70 interaction and substrate-binding properties to complement various yeast Hsp40-dependent functions
    Shruthi S Vembar
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    J Biol Chem 284:32462-71. 2009
    ..Taken together, these data suggest that the substrate binding properties of certain JDPs, not simply the formation of unique Hsp70-JDP pairs, are critical to specify in vivo function...
  35. ncbi request reprint Molecular pathogenesis of alpha-1-antitrypsin deficiency-associated liver disease: a meeting review
    David H Perlmutter
    Department of Pediatrics, University of Pittsburgh School of Medicine, Children s Hospital of Pittsburgh, Pittsburgh, PA 15213, USA
    Hepatology 45:1313-23. 2007
    ..A session of the meeting was devoted to novel therapeutic strategies being developed for AT deficiency as well as to strategies either in development or already being applied to the class of diseases associated with mutant proteins...
  36. ncbi request reprint Yeast as a model system to investigate protein conformational diseases
    Christina M Coughlan
    Department of Biological Sciences, University of Pittsburgh, PA, USA
    Methods Mol Biol 232:77-90. 2003
  37. ncbi request reprint HspBP1, a homologue of the yeast Fes1 and Sls1 proteins, is an Hsc70 nucleotide exchange factor
    Mehdi Kabani
    Department of Biological Sciences, 274 Crawford Hall, University of Pittsburgh, Pittsburgh, PA 15260, USA
    FEBS Lett 531:339-42. 2002
    ..Together, our data indicate that HspBP1 is a member of this new class of nucleotide exchange factors that exhibit varying degrees of compartment and species specificity...
  38. pmc The endoplasmic reticulum-associated degradation of the epithelial sodium channel requires a unique complement of molecular chaperones
    Teresa M Buck
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Mol Biol Cell 21:1047-58. 2010
    ..Our data indicate that Hsp40s can act independently of Hsp70 to select substrates for ERAD...
  39. doi request reprint Identification of Hsp70 modulators through modeling of the substrate binding domain
    Conor M Haney
    Department of Pharmacy, University of Pittsburgh, Pittsburgh, USA
    Bioorg Med Chem Lett 19:3828-31. 2009
    ..The design, synthesis and preliminary activity of small molecular weight modulators of the heat shock protein 70 (Hsp70) are described. The compounds provide a starting point for the synthesis of novel tools to decipher Hsp70 biology...
  40. pmc Saccharomyces cerivisiae as a model system for kidney disease: what can yeast tell us about renal function?
    Alexander R Kolb
    University of Pittsburgh, 4249 Fifth Ave, A320 Langley Hall, Pittsburgh, PA 15260, USA
    Am J Physiol Renal Physiol 301:F1-11. 2011
    ..These attributes validate yeast as a model system to explore renal physiology and suggest that research initiated using this system may lead to novel therapeutics...
  41. pmc The yeast Hsp110, Sse1p, exhibits high-affinity peptide binding
    Jennifer L Goeckeler
    University of Pittsburgh, Department of Biological Sciences, 274A Crawford Hall, Pittsburgh, PA 15260, USA
    FEBS Lett 582:2393-6. 2008
    ..Moreover, an Hsp70-binding peptide is unable to associate with Sse1p, suggesting that Hsp70s and Hsp110s possess partially distinct peptide recognition motifs...
  42. pmc Pyrimidinone-peptoid hybrid molecules with distinct effects on molecular chaperone function and cell proliferation
    Christine M Wright
    Department of Biological Sciences, University of Pittsburgh, 274 Crawford Hall, Pittsburgh, PA 15260, USA
    Bioorg Med Chem 16:3291-301. 2008
    ..In many cases, anti-proliferative activity correlated with inhibition of J domain stimulation of Hsp70...
  43. ncbi request reprint Use of yeast as a model system to investigate protein conformational diseases
    Christina M Coughlan
    Department of Biological Sciences, University of Denver, Denver, CO 80208, USA
    Mol Biotechnol 30:171-80. 2005
    ..In this review, we describe what has been learned from these studies, and speculate on future uses of yeast expression systems...
  44. ncbi request reprint CFTR expression and ER-associated degradation in yeast
    Yimao Zhang
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA, USA
    Methods Mol Med 70:257-65. 2002
  45. ncbi request reprint Roles of molecular chaperones in endoplasmic reticulum (ER) quality control and ER-associated degradation (ERAD)
    Shuh ichi Nishikawa
    Department of Chemistry, Graduate School of Science, Nagoya University, Nagoya 464 8602
    J Biochem 137:551-5. 2005
    ..One important role for chaperones during ERAD is to prevent substrate protein aggregation. Substrate selection is another important role for molecular chaperones during ERAD...
  46. ncbi request reprint Distinct machinery is required in Saccharomyces cerevisiae for the endoplasmic reticulum-associated degradation of a multispanning membrane protein and a soluble luminal protein
    Gregory Huyer
    Department of Cell Biology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 279:38369-78. 2004
    ....
  47. ncbi request reprint Autophagy: an ER protein quality control process
    Kristina B Kruse
    Biology Department, University of Nevada, Reno, Nevada 89557, USA
    Autophagy 2:135-7. 2006
    ....
  48. pmc Real-time fluorescence detection of ERAD substrate retrotranslocation in a mammalian in vitro system
    Judit Wahlman
    Department of Biochemistry and Biophysics, Texas A and M University, College Station, TX 77843, USA
    Cell 129:943-55. 2007
    ..In addition, pro-alpha factor photocrosslinked derlin-1, but not Sec61alpha. Thus, derlin-1 appears to be involved in pro-alpha factor retrotranslocation...
  49. ncbi request reprint Apolipoprotein B100 exit from the endoplasmic reticulum (ER) is COPII-dependent, and its lipidation to very low density lipoprotein occurs post-ER
    Viktoria Gusarova
    Department of Medicine and The Cardiovascular Institute, Mount Sinai School of Medicine, New York, New York 10029, USA
    J Biol Chem 278:48051-8. 2003
    ..We conclude that apoB100-lipoproteins exit the ER in COPII vesicles, but under conditions favorable for VLDL formation final lipid loading occurs post-ER...
  50. pmc The transmembrane domain is sufficient for Sbh1p function, its association with the Sec61 complex, and interaction with Rtn1p
    Dejiang Feng
    VTT Biotechnology, FI 02044 VTT, Espoo, Finland
    J Biol Chem 282:30618-28. 2007
    ....
  51. ncbi request reprint Evolving questions and paradigm shifts in endoplasmic-reticulum-associated degradation (ERAD)
    Ardythe A McCracken
    Biology Department, University of Nevada, Reno, Nevada 89557, USA
    Bioessays 25:868-77. 2003
    ..Here we review the molecular mechanisms of ERAD, its role in metabolic regulation and biomedical implications, and the unanswered questions regarding this process...
  52. pmc The molecular mechanisms underlying BiP-mediated gating of the Sec61 translocon of the endoplasmic reticulum
    Nathan N Alder
    Department of Medical Biochemistry and Genetics, Texas A and M University System Health Science Center, College Station, TX 77843, USA
    J Cell Biol 168:389-99. 2005
    ..The mechanism by which BiP mediates translocon pore closure and opening is therefore similar to that in which Hsp70 chaperones associate with and dissociate from substrates...
  53. ncbi request reprint The function of the yeast molecular chaperone Sse1 is mechanistically distinct from the closely related hsp70 family
    Lance Shaner
    Department of Microbiology and Molecular Genetics, University of Texas Houston Medical School, Houston, Texas 77030, USA
    J Biol Chem 279:21992-2001. 2004
    ..These data represent the first structure-function analysis of this abundant but ill defined chaperone, and establish several novel aspects of Sse1/Hsp110 function relative to Hsp70...
  54. ncbi request reprint Differential requirements of novel A1PiZ degradation deficient (ADD) genes in ER-associated protein degradation
    Elizabeth A Palmer
    Biology Department, University of Nevada, Reno, NV 89557, USA
    J Cell Sci 116:2361-73. 2003
    ..Finally, we report on bioinformatic analyses of the new Add proteins, which will lead to testable models to elucidate their activities...
  55. ncbi request reprint Golgi-associated maturation of very low density lipoproteins involves conformational changes in apolipoprotein B, but is not dependent on apolipoprotein E
    Viktoria Gusarova
    Department of Medicine, Leon Charney Division of Cardiology, New York University School of Medicine, New York, New York 10016, USA
    J Biol Chem 282:19453-62. 2007
    ..We conclude that VLDL maturation in the Golgi involves apoB conformational changes and that the expansion of the lipoprotein does not require apoE; rather, the increase in VLDL surface area favors apoE binding...
  56. pmc Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes
    Daniel J Klionsky
    Life Sciences Institute, University of Michigan, Ann Arbor, Michigan 48109 2216, USA
    Autophagy 4:151-75. 2008
    ..In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to verify an autophagic response...
  57. pmc Cdc37 has distinct roles in protein kinase quality control that protect nascent chains from degradation and promote posttranslational maturation
    Atin K Mandal
    Department of Pharmacology and Biological Chemistry, Mount Sinai School of Medicine, New York, NY 10029, USA
    J Cell Biol 176:319-28. 2007
    ..Our studies demonstrate that Cdc37 has a general role in kinome biogenesis...
  58. ncbi request reprint Selective compounds define Hsp90 as a major inhibitor of apoptosis in small-cell lung cancer
    Anna Rodina
    Program in Molecular Pharmacology and Chemistry, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, New York 10021, USA
    Nat Chem Biol 3:498-507. 2007
    ..These results provide important evidence for a transformation-specific interplay between chaperones in regulating apoptosis in malignant cells...
  59. pmc Design of a fluorescence polarization assay platform for the study of human Hsp70
    Yanlong Kang
    Program in Molecular Pharmacology and Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Bioorg Med Chem Lett 18:3749-51. 2008
    ..Here, we present a novel assay platform based on fluorescence polarization that is suitable for investigating the yet elusive molecular mechanics of human Hsp70 allosteric regulation...
  60. pmc Characterization of an ERAD gene as VPS30/ATG6 reveals two alternative and functionally distinct protein quality control pathways: one for soluble Z variant of human alpha-1 proteinase inhibitor (A1PiZ) and another for aggregates of A1PiZ
    Kristina B Kruse
    Department of Biology, University of Nevada, Reno, NV 89557, USA
    Mol Biol Cell 17:203-12. 2006
    ..These findings illustrate the complex nature of protein quality control in the secretion pathway and reveal multiple sites that recognize and sort both soluble and aggregated forms of aberrant or misfolded proteins...
  61. pmc A precursor-specific role for Hsp40/Hsc70 during tail-anchored protein integration at the endoplasmic reticulum
    Catherine Rabu
    Faculty of Life Sciences, University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, United Kingdom
    J Biol Chem 283:27504-13. 2008
    ....

Research Grants8

  1. Endoplasmic Reticulum (ER) Associated Degradation of Membrane Proteins in Yeast
    JEFFREY BRODSKY; Fiscal Year: 2007
    ....
  2. Endoplasmic Reticulum (ER) Associated Degradation of Membrane Proteins in Yeast
    JEFFREY BRODSKY; Fiscal Year: 2009
    ....
  3. Modulators of T Antigen-Mediated Chaperone Action
    JEFFREY BRODSKY; Fiscal Year: 2004
    ..abstract_text> ..
  4. Endoplasmic Reticulum (ER) Associated Degradation of Membrane Proteins in Yeast
    Jeffrey L Brodsky; Fiscal Year: 2010
    ..The knowledge gained from these studies may lead to the development of novel therapies and provide methods to define the pathway of disease onset. ..
  5. The CFTR Degradation Pathway in Yeast
    JEFFREY BRODSKY; Fiscal Year: 2002
    ....
  6. ER Associated Degradation of Membrane Proteins in Yeast
    JEFFREY BRODSKY; Fiscal Year: 2006
    ....