Research Topics
| Jennifer BrodbeltSummaryAffiliation: University of Texas Country: USA Publications
Research Grants
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Detail Information
Publications
Screening of threading bis-intercalators binding to duplex DNA by electrospray ionization tandem mass spectrometryCarolyn L Mazzitelli
Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, Texas 78712 0165, USA
J Am Soc Mass Spectrom 18:311-21. 2007....
Evaluation of DNA/Ligand interactions by electrospray ionization mass spectrometryJennifer S Brodbelt
Department of Chemistry and Biochemistry, University of Texas, Austin, 78712, USA
Annu Rev Anal Chem (Palo Alto Calif) 3:67-87. 2010..Chemical probe methods applied to DNA/ligand complexes with ESI-MS detection afford information about ligand-binding sites and conformational changes of DNA that occur upon ligand binding...- Shedding light on the frontier of photodissociationJennifer S Brodbelt
Department of Chemistry and Biochemistry, University of Texas, Austin, TX 78712, USA
J Am Soc Mass Spectrom 22:197-206. 2011..Moreover, the discussion will emphasize photodissociation in quadrupole ion traps, because that platform has been used for one of the greatest arrays of new applications over the past decade... - Infrared multiphoton dissociation in quadrupole ion trapsJennifer S Brodbelt
Department of Chemistry and Biochemistry, University of Texas, Austin, TX 78712, USA
Mass Spectrom Rev 28:390-424. 2009..Numerous applications of IRMPD are discussed as well, including ones related to the analysis of drugs, peptides, nucleic acids, and oligosaccharides... 
Novel guanosine-cytidine dinucleoside that self-assembles into a trimeric supramoleculeJonathan L Sessler
Department of Chemistry and Biochemistry and Institute of Cellular and Molecular Biology, 1 University Station A5300, The University of Texas at Austin, 78712 0165, USA
Org Lett 5:2627-30. 2003..Dinucleoside 1 was obtained by utilizing two consecutive palladium-catalyzed cross-coupling reactions. Ensemble I was analyzed by ESI-MS, NMR spectroscopies, size exclusion chromatography (SEC), and vapor pressure osmometry (VPO)...- Enhancing Hoogsteen interactions: a pyrrole-containing purine nucleoside that competes with guanosine self-assemblyJonathan L Sessler
Department of Chemistry and Biochemistry and Institute for Cellular and Molecular Biology, 1 University Station A5300, The University of Texas at Austin, Austin, TX 78712 0165, USA
J Am Chem Soc 126:11460-1. 2004..Nucleoside 1 is shown to bind guanosine 10 (a classic ADD motif) to form ensemble I. This interaction competes effectively with guanosine self-assembly and, as such, is capable of disrupting guanosine quadruplex formation... - Duplex and quadruplex DNA binding and photocleavage by trioxatriangulenium ionArti Pothukuchy
Division of Medicinal Chemistry and Institute for Cellular and Molecular Biology, The University of Texas, Austin, Texas 78712, USA
Biochemistry 44:2163-72. 2005..Analysis by denaturing polyacrylamide gel electrophoresis reveals significantly less TOTA photocleavage of these quadruplex telomeric repeats when compared to the duplex repeats... - Probing ligand binding to duplex DNA using KMnO4 reactions and electrospray ionization tandem mass spectrometryCarolyn L Mazzitelli
Department of Chemistry and Biochemistry, 1 University Station A5300, University of Texas at Austin, Austin, Texas 78712, USA
Anal Chem 79:4636-47. 2007..Collisional activated dissociation (CAD) and infrared multiphoton dissociation (IRMPD) experiments were used to determine the site of oxidation based on oligonucleotide fragmentation patterns... - Investigation of quadruplex oligonucleotide-drug interactions by electrospray ionization mass spectrometryWendi M David
Department of Chemistry and Biochemistry, College of Pharmacy, University of Texas at Austin, 78712, USA
Anal Chem 74:2029-33. 2002..These ESI-MS results are applicable to both the identification and characterization of G-quadruplex interactive agents and may also be useful in probing unusual DNA structures... - ESI-MS characterization of a novel pyrrole-inosine nucleoside that interacts with guanine basesSarah E Pierce
Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, TX 78712 1167, United States
Anal Chim Acta 627:129-35. 2008..The specific binding of 1 to guanine-rich DNA emphasizes the necessity of careful ligand design for specific sequence recognition... - Infrared multiphoton dissociation of duplex DNA/drug complexes in a quadrupole ion trapJeffrey J Wilson
Department of Chemistry and Biochemistry, 1 University Station A5300, University of Texas at Austin, Austin, Texas 78712, USA
Anal Chem 79:2067-77. 2007.... - Top-down protein fragmentation by infrared multiphoton dissociation in a dual pressure linear ion trapJames A Madsen
Department of Chemistry and Biochemistry, The University of Texas at Austin, 1 University Station A5300, Austin, Texas 78712, USA
Anal Chem 81:8677-86. 2009.... - Evaluation of binding of perylene diimide and benzannulated perylene diimide ligands to DNA by electrospray ionization mass spectrometryCarolyn L Mazzitelli
Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin, Texas 58712-0165, USA
J Am Soc Mass Spectrom 17:593-604. 2006..Our results indicate that Tel01, Tel12, and Tel34 are the most selective for G-quadruplex DNA... - Differentiation of phosphorylated and unphosphorylated peptides by high-performance liquid chromatography-electrospray ionization-infrared multiphoton dissociation in a quadrupole ion trapMatthew C Crowe
Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, Texas 78712, USA
Anal Chem 77:5726-34. 2005..The ability of this technique to differentiate peptides based on phosphorylation state is unaffected by whether the peptides are protonated or sodium-cationized... - Infrared multiphoton dissociation of peptide cations in a dual pressure linear ion trap mass spectrometerMyles W Gardner
Department of Chemistry and Biochemistry, The University of Texas at Austin, 1 University Station A5300, Austin, Texas 78751, USA
Anal Chem 81:8109-18. 2009..Highly charged primary product ions also underwent efficient photodissociation to yield singly charged secondary product ions, thus simplifying the IRMPD product ion mass spectra... - Interactions of sulfur-containing acridine ligands with DNA by ESI-MSSuncerae I Smith
Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, TX 78712, USA
Analyst 134:2058-66. 2009..At higher irradiation times, a variety of sequence ions were observed, some retaining the AS4/AN1 ligand, which was indicative of covalent binding... - Impact of proline and aspartic acid residues on the dissociation of intermolecularly crosslinked peptidesMyles W Gardner
Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin, Texas 78712, USA
J Am Soc Mass Spectrom 19:344-57. 2008.... - Photodissociation of non-covalent peptide-crown ether complexesJeffrey J Wilson
Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin, Texas 78712, USA
J Am Soc Mass Spectrom 19:257-60. 2008.... - Sequencing and characterization of oligosaccharides using infrared multiphoton dissociation and boronic acid derivatization in a quadrupole ion trapMichael Pikulski
Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin, Texas 78712 0165, USA
J Am Soc Mass Spectrom 18:2094-106. 2007..We demonstrate this approach for two series of oligosaccharides, the lacto-N-fucopentaoses (LNFPs) and the lacto-N-difucohexaoses (LNDFHs)... - MS/MS simplification by 355 nm ultraviolet photodissociation of chromophore-derivatized peptides in a quadrupole ion trapJeffrey J Wilson
Department of Chemistry and Biochemistry, University of Texas at Austin, 1 University Station A5300, Austin, Texas 78712, USA
Anal Chem 79:7883-92. 2007..The abundances of chromophore-containing a/b fragment ions follow a quadratic trend due to the extensive secondary fragmentation at higher laser energies or multiple pulses... - Evaluation of metal-mediated DNA binding of benzoxazole ligands by electrospray ionization mass spectrometryCarolyn L Mazzitelli
Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin, Texas 78712, USA
J Am Soc Mass Spectrom 19:209-18. 2008..Metal ions also enhanced the DNA binding of the ligands with the long side chains, especially for 9, which also exhibited the highest level of cytotoxicity of the long side-chain compounds... - Asymmetric charge partitioning upon dissociation of DNA duplexesJames A Madsen
Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin, Texas 78712 0165, USA
J Am Soc Mass Spectrom 21:1144-50. 2010..Comparisons were made with the dissociation behavior previously studied for protein noncovalent complexes and past studies of the gas-phase conformations and dissociation of DNA complexes... - Electrospray ionization of nucleic acid aptamer/small molecule complexes for screening aptamer selectivityKarin M Keller
Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin TX 78712, USA
J Mass Spectrom 40:1327-37. 2005..These results indicate that in at least some cases, mass spectrometric data on aptamer/ligand binding affinities should be used in conjunction with complementary techniques to fully assess aptamer molecular recognition properties... - Influence of initial charge state on fragmentation patterns for noncovalent drug/DNA duplex complexesKarin M Keller
Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin TX 78712, USA
J Mass Spectrom 40:1362-71. 2005..The dissociation pathways for the lower charge state complexes are probably more reflective of specific drug-DNA interactions because Coulombic and/or conformational effects are less marked for these precursors... - Infrared multiphoton dissociation of small-interfering RNA anions and cationsMyles W Gardner
Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin, Texas 78712 0165, USA
J Am Soc Mass Spectrom 21:580-91. 2010..With longer irradiation times, however, the individual single-strands underwent secondary dissociation to yield informative sequence ions not obtained by CID... - Collisionally activated dissociation and infrared multiphoton dissociation of oligonucleotides in a quadrupole ion trapKarin M Keller
Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin, TX 78712, USA
Anal Biochem 326:200-10. 2004..For this reason IRMPD can sometimes facilitate analysis of sequences containing modified bases... - Reduction of chemical noise in electrospray ionization mass spectrometry by supplemental IR activationMyles W Gardner
Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin, Texas 78712 0165, USA
J Am Soc Mass Spectrom 20:2206-10. 2009..Collision activation was not as effective for desolvating the ions present in the chemical noise... - Characterization of erythromycin analogs by collisional activated dissociation and infrared multiphoton dissociation in a quadrupole ion trapMatthew C Crowe
Department of Chemistry and Biochemistry, University of Texas at Austin 78712-1167, USA
J Am Soc Mass Spectrom 13:630-49. 2002..Multi-step IRMPD allows mapping of the fragmentation genealogies in greater detail and supports the proposed structures of the fragment ions... - Ultrafast ultraviolet photodissociation at 193 nm and its applicability to proteomic workflowsJames A Madsen
Department of Chemistry and Biochemistry, The University of Texas at Austin, 1 University Station A5300, Austin, Texas 78712, USA
J Proteome Res 9:4205-14. 2010.... - Preferential cleavage of N-N hydrazone bonds for sequencing bis-arylhydrazone conjugated peptides by electron transfer dissociationMyles W Gardner
Department of Chemistry and Biochemistry, The University of Texas at Austin, 1 University Station A5300, Austin, Texas 78712, USA
Anal Chem 82:5751-9. 2010.... - Rapid and selective screening for sulfhydryl analytes in plasma and urine using surface-enhanced transmission mode desorption electrospray ionization mass spectrometryJoseph E Chipuk
Department of Chemistry and Biochemistry, University of Texas at Austin, University Station A5300, Austin, Texas 78712, USA
Anal Chem 82:4130-9. 2010..Integration of the chemoselective capture materials with ambient ionization and tandem mass spectrometry results in a powerful combination of speed and selectivity for targeted analyte screening... - Differentiation of flavonoid glycoside isomers by using metal complexation and electrospray ionization mass spectrometryMichael Pikulski
Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin, Texas 78712-0165, USA
J Am Soc Mass Spectrom 14:1437-53. 2003..Using this technique, three groups of flavonoid glycoside isomers are differentiated, including glycosides of apigenin, quercetin, and luteolin... - Characterization of aziridinylbenzoquinone DNA cross-links by liquid chromatography-infrared multiphoton dissociation-mass spectrometrySarah E Pierce
Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin, Texas 78712, USA
Chem Res Toxicol 23:1097-104. 2010.... - Ultraviolet photodissociation mass spectrometry of bis-aryl hydrazone conjugated peptidesMyles W Gardner
Department of Chemistry and Biochemistry, The University of Texas at Austin, 1 University Station A5300, Austin, Texas 78712 1167, USA
Anal Chem 81:4864-72. 2009..Ultraviolet photodissociation of BAH-cross-linked peptides also yielded more diagnostic sequence ions than CID to unambiguously locate the site of conjugation... - Simplifying fragmentation patterns of multiply charged peptides by N-terminal derivatization and electron transfer collision activated dissociationJames A Madsen
Department of Chemistry and Biochemistry, The University of Texas at Austin, 1 University Station A5300, Austin, Texas 78712, USA
Anal Chem 81:3645-53. 2009..This improved LC-MS(n) strategy was demonstrated for a variety of multiply charged model peptides and a tryptic digest of myoglobin... - Gas-phase hydrogen/deuterium exchange and conformations of deprotonated flavonoids and gas-phase acidities of flavonoidsJunmei Zhang
Department of Chemistry and Biochemistry, University of Texas, Austin, Texas 78712, USA
J Am Chem Soc 126:5906-19. 2004.... - MS(n) characterization of protonated cyclic peptides and metal complexesSheldon M Williams
Department of Chemistry and Biochemistry, The University of Texas at Austin, 78712, USA
J Am Soc Mass Spectrom 15:1039-54. 2004..For cyclic lipopeptides, complexes with silver, nickel and strontium ions provided enhanced abundances of key fragment ions... - Infrared multiphoton dissociation for enhanced de novo sequence interpretation of N-terminal sulfonated peptides in a quadrupole ion trapJeffrey J Wilson
Department of Chemistry and Biochemistry, 1 University Station A5300, University of Texas at Austin, Austin, Texas 78712, USA
Anal Chem 78:6855-62. 2006..Energy-variable CAD studies confirmed a 30-40% reduction in the critical energies of the N-terminal sulfonated peptides relative to unmodified peptides. This reduction in dissociation energy facilitates IRMPD in a quadrupole ion trap... - Threshold dissociation and molecular modeling of transition metal complexes of flavonoidsJunmei Zhang
Department of Chemistry and Biochemistry, University of Texas, Austin, Texas 78712-0165, USA
J Am Soc Mass Spectrom 16:139-51. 2005..The conformations were combined with the point charges and helium accessible surface areas to explain qualitatively the differences in threshold energies for isomeric flavonoids... - Tunable transition metal-ligand complexation for enhanced elucidation of flavonoid diglycosides by electrospray ionization mass spectrometryMichael Pikulski
Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin, Texas 78712 0165, USA
J Am Soc Mass Spectrom 18:422-31. 2007..Upon collisionally activated dissociation, the complexes yield fragmentation patterns in which the abundances of key diagnostic ions are enhanced, thus facilitating isomer differentiation... - Evaluation of complexes of DNA duplexes and novel benzoxazoles or benzimidazoles by electrospray ionization mass spectrometryLeon Oehlers
Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin, Texas 78712-0165, USA
J Am Soc Mass Spectrom 15:1593-603. 2004..Complexation between DNA and the biologically inactive analog 1 was not observed, either in the absence or presence of metal cations... - Evaluation of relative DNA binding affinities of anthrapyrazoles by electrospray ionization mass spectrometrySuncerae I Smith
Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, TX 78712, USA
J Mass Spectrom 42:681-8. 2007..However, for complexes containing AP2 or mitoxantrone, strand separation with the ligand remaining on one of the single strands was observed, indicative of a different binding mode or stronger binding... - Infrared multiphoton dissociation (IRMPD) and collisionally activated dissociation of peptides in a quadrupole ion trap with selective IRMPD of phosphopeptidesMatthew C Crowe
Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin, Texas 78712-0165, USA
J Am Soc Mass Spectrom 15:1581-92. 2004..Finally, the selectivity of the IRMPD technique for phosphorylated species in complex mixtures is confirmed with the analysis of a mock peptide mixture and a tryptic digest of alpha-casein... - Determination of the glycosylation site of flavonoid monoglucosides by metal complexation and tandem mass spectrometryBarry D Davis
Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin, Texas 78712-0165, USA
J Am Soc Mass Spectrom 15:1287-99. 2004.... - Chromogenic cross-linker for the characterization of protein structure by infrared multiphoton dissociation mass spectrometryMyles W Gardner
Department of Chemistry and Biochemistry, The University of Texas at Austin, 1 University Station A5300, Austin, Texas, USA 78712, USA
Anal Chem 80:4807-19. 2008.... - Gas-phase stability of G-quadruplex DNA determined by electrospray ionization tandem mass spectrometry and molecular dynamics simulationsCarolyn L Mazzitelli
Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, Texas 78712, USA
J Am Soc Mass Spectrom 18:1760-73. 2007..This discrepancy is attributed to differences in the fragmentation pathway of the quadruplex... - Improved infrared multiphoton dissociation of peptides through N-terminal phosphonite derivatizationLisa A Vasicek
Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin, Texas 78712, USA
J Am Soc Mass Spectrom 20:377-84. 2009..We compare the effectiveness of this derivatization/IRMPD approach to that of a common N-terminal sulfonation reaction that utilizes 4-sulfophenylisothiocyanate (SPITC) in conjunction with CID and IRMPD... - Comparison of infrared multiphoton dissociation and collision-induced dissociation of supercharged peptides in ion trapsJames A Madsen
Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin, Texas 78712 0165, USA
J Am Soc Mass Spectrom 20:349-58. 2009..The enhanced sequence coverage afforded by IRMPD of supercharged ions was demonstrated for a variety of model peptides, as well as for a tryptic digest of cytochrome c... - Enhanced electron transfer dissociation through fixed charge derivatization of cysteinesLisa Vasicek
Department of Chemistry and Biochemistry, The University of Texas at Austin, 1 University Station A5300, Austin, Texas 78712, USA
Anal Chem 81:7876-84. 2009..In addition, the formation of product ions in multiple charge states upon ETD is minimized for the APTA-modified peptides...
Research Grants
- Design and Characterization of DNA Interactive AgentsJennifer S Brodbelt; Fiscal Year: 2010..This research enables the design of new anticancer aziridinylbenzoquinones and advances the analysis of DNA-drug adducts by innovative mass spectrometric strategies. ..
- Design and Characterization of DNA Interactive AgentsJennifer Brodbelt; Fiscal Year: 2005..The anticancer and antibacterial effects of UK-1 and A-62176 analogs will be examined in a range of human cancer cell lines and Gram negative and Gram positive bacteria. ..
- Analysis of Phenolic Phytochemical by ESI-MSJennifer Brodbelt; Fiscal Year: 2003....
- Design and Characterization of DNA Interactive AgentsJennifer S Brodbelt; Fiscal Year: 2011..This research enables the design of new anticancer aziridinylbenzoquinones and advances the analysis of DNA-drug adducts by innovative mass spectrometric strategies. ..