DAVID BRAUTIGAN

Summary

Affiliation: University of Virginia
Country: USA

Publications

  1. pmc Activation of Aurora-A kinase by protein phosphatase inhibitor-2, a bifunctional signaling protein
    David L Satinover
    Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    Proc Natl Acad Sci U S A 101:8625-30. 2004
  2. ncbi request reprint Chromium and vanadate combination increases insulin-induced glucose uptake by 3T3-L1 adipocytes
    David L Brautigan
    Center for Cell Signaling and Department of Microbiology, University of Virginia School of Medicine, Charlottesville, 22908, USA
    Biochem Biophys Res Commun 347:769-73. 2006
  3. pmc α-SNAP inhibits AMPK signaling to reduce mitochondrial biogenesis and dephosphorylates Thr172 in AMPKα in vitro
    Lifu Wang
    Department of Microbiology, Immunology and Cancer Biology, Center for Cell Signaling, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
    Nat Commun 4:1559. 2013
  4. pmc Protein Ser/Thr phosphatase-6 is required for maintenance of E-cadherin at adherens junctions
    Takashi Ohama
    Center for Cell Signaling and Department of Microbiology, Immunology, and Cancer Biology, University of Virginia School of Medicine, Box 800577, West Complex MSB 7225, Charlottesville, Virginia 22908, USA
    BMC Cell Biol 14:42. 2013
  5. pmc Identification and characterization of two distinct PPP1R2 isoforms in human spermatozoa
    Luís Korrodi-Gregório
    Laboratory of Signal Transduction, Centre for Cell Biology, Biology Department, University of Aveiro, 3810 193 Aveiro, Portugal
    BMC Cell Biol 14:15. 2013
  6. doi request reprint Protein Ser/Thr phosphatases--the ugly ducklings of cell signalling
    David L Brautigan
    Department of Microbiology, Immunology and Cancer Biology, Center for Cell Signaling, University of Virginia, School of Medicine, Charlottesville, VA 22908, USA
    FEBS J 280:324-45. 2013
  7. pmc Mitotic phosphorylation activates hepatoma-derived growth factor as a mitogen
    Allen D Everett
    Department of Pediatrics, Cardiology Division, Johns Hopkins University, 600 N Wolfe Street, Baltimore, MD 21287, USA
    BMC Cell Biol 12:15. 2011
  8. pmc Phosphatase inhibitor 2 promotes acetylation of tubulin in the primary cilium of human retinal epithelial cells
    Weiping Wang
    Center for Cell Signaling, University of Virginia, School of Medicine, Charlottesville, 22908, USA
    BMC Cell Biol 9:62. 2008
  9. pmc Mapping of protein phosphatase-6 association with its SAPS domain regulatory subunit using a model of helical repeats
    Julien Guergnon
    Center for Cell Signalling, University of Virginia School of Medicine, Charlottesville, VA 22908 USA
    BMC Biochem 10:24. 2009
  10. ncbi request reprint Allosteric activation of protein phosphatase 2C by D-chiro-inositol-galactosamine, a putative mediator mimetic of insulin action
    D L Brautigan
    Center for Cell Signaling, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
    Biochemistry 44:11067-73. 2005

Research Grants

  1. CHROMIUM ENHANCEMENT OF INSULIN SIGNALING
    DAVID BRAUTIGAN; Fiscal Year: 2005
  2. Phosphorylation and Function of Inhibitor-2
    DAVID BRAUTIGAN; Fiscal Year: 2005
  3. RAPAMYCIN BLOCKADE OF PROTEIN PHOSPHATASE SIGNALING
    DAVID BRAUTIGAN; Fiscal Year: 2005
  4. CHROMIUM ENHANCEMENT OF INSULIN SIGNALING
    DAVID BRAUTIGAN; Fiscal Year: 2006
  5. RAPAMYCIN BLOCKADE OF PROTEIN PHOSPHATASE SIGNALING
    DAVID BRAUTIGAN; Fiscal Year: 2006
  6. RAPAMYCIN BLOCKADE OF PROTEIN PHOSPHATASE SIGNALING
    DAVID BRAUTIGAN; Fiscal Year: 2007
  7. CORE--MICROSCOPY
    DAVID BRAUTIGAN; Fiscal Year: 2007
  8. Phosphorylation & Function of Inhibitor-2
    DAVID BRAUTIGAN; Fiscal Year: 2009
  9. Phosphorylation & Function of Inhibitor-2
    DAVID BRAUTIGAN; Fiscal Year: 2009
  10. RAPAMYCIN BLOCKADE OF PROTEIN PHOSPHATASE SIGNALING
    DAVID BRAUTIGAN; Fiscal Year: 2004

Collaborators

Detail Information

Publications67

  1. pmc Activation of Aurora-A kinase by protein phosphatase inhibitor-2, a bifunctional signaling protein
    David L Satinover
    Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    Proc Natl Acad Sci U S A 101:8625-30. 2004
    ..These results suggest that both I-2 and TPX2 function as allosteric activators of Aurora-A. This implies that I-2 is a bifunctional signaling protein with separate domains to inhibit PP1 and directly stimulate Aurora-A kinase...
  2. ncbi request reprint Chromium and vanadate combination increases insulin-induced glucose uptake by 3T3-L1 adipocytes
    David L Brautigan
    Center for Cell Signaling and Department of Microbiology, University of Virginia School of Medicine, Charlottesville, 22908, USA
    Biochem Biophys Res Commun 347:769-73. 2006
    ..These data show that chromium enhances glucose uptake even when Tyr phosphorylation levels are elevated by vanadate plus insulin, suggesting separate mechanisms of action for vanadate and chromium...
  3. pmc α-SNAP inhibits AMPK signaling to reduce mitochondrial biogenesis and dephosphorylates Thr172 in AMPKα in vitro
    Lifu Wang
    Department of Microbiology, Immunology and Cancer Biology, Center for Cell Signaling, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
    Nat Commun 4:1559. 2013
    ..The mouse α-SNAP mutant hyh (hydrocephalus with hop gait) shows enhanced binding and inhibition of AMPK. By negatively controlling AMPK, α-SNAP therefore potentially coordinates membrane trafficking and metabolism...
  4. pmc Protein Ser/Thr phosphatase-6 is required for maintenance of E-cadherin at adherens junctions
    Takashi Ohama
    Center for Cell Signaling and Department of Microbiology, Immunology, and Cancer Biology, University of Virginia School of Medicine, Box 800577, West Complex MSB 7225, Charlottesville, Virginia 22908, USA
    BMC Cell Biol 14:42. 2013
    ..Signaling pathways regulate E-cadherin function and cellular distribution via phosphorylation of the cytoplasmic region by kinases such as casein kinases but the protein phosphatases involved have not been identified...
  5. pmc Identification and characterization of two distinct PPP1R2 isoforms in human spermatozoa
    Luís Korrodi-Gregório
    Laboratory of Signal Transduction, Centre for Cell Biology, Biology Department, University of Aveiro, 3810 193 Aveiro, Portugal
    BMC Cell Biol 14:15. 2013
    ..Preliminary results have shown a protein phosphatase inhibitor activity resembling PPP1R2 in bovine and primate spermatozoa...
  6. doi request reprint Protein Ser/Thr phosphatases--the ugly ducklings of cell signalling
    David L Brautigan
    Department of Microbiology, Immunology and Cancer Biology, Center for Cell Signaling, University of Virginia, School of Medicine, Charlottesville, VA 22908, USA
    FEBS J 280:324-45. 2013
    ..Signalling networks and modelling schemes need to incorporate the full gamut of protein Ser/Thr phosphatases and their interconnections...
  7. pmc Mitotic phosphorylation activates hepatoma-derived growth factor as a mitogen
    Allen D Everett
    Department of Pediatrics, Cardiology Division, Johns Hopkins University, 600 N Wolfe Street, Baltimore, MD 21287, USA
    BMC Cell Biol 12:15. 2011
    ..Mass spectrometry based methods have identified HDGF as a phosphoprotein without validation or a functional consequence of this post-translational modification...
  8. pmc Phosphatase inhibitor 2 promotes acetylation of tubulin in the primary cilium of human retinal epithelial cells
    Weiping Wang
    Center for Cell Signaling, University of Virginia, School of Medicine, Charlottesville, 22908, USA
    BMC Cell Biol 9:62. 2008
    ..Microtubules make up the axoneme of cilia and are selectively acetylated and this is thought to contribute to the stability of the structure. However, mechanisms to regulate tubulin acetylation in cilia are poorly understood...
  9. pmc Mapping of protein phosphatase-6 association with its SAPS domain regulatory subunit using a model of helical repeats
    Julien Guergnon
    Center for Cell Signalling, University of Virginia School of Medicine, Charlottesville, VA 22908 USA
    BMC Biochem 10:24. 2009
    ..These subunits are conserved, and three human SAPS-related proteins are known to associate with PP6 phosphatase, the Sit4 human homologue...
  10. ncbi request reprint Allosteric activation of protein phosphatase 2C by D-chiro-inositol-galactosamine, a putative mediator mimetic of insulin action
    D L Brautigan
    Center for Cell Signaling, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
    Biochemistry 44:11067-73. 2005
    ..We propose that INS2 allosterically activates PP2C, fulfilling the role of a putative mediator mimetic of insulin signaling to promote protein dephosphorylation and metabolic responses...
  11. ncbi request reprint A novel transmembrane Ser/Thr kinase complexes with protein phosphatase-1 and inhibitor-2
    Hong Wang
    Center for Cell Signaling, University of Virginia School of Medicine, Charlottesville, VA 22908 0577, USA
    J Biol Chem 277:49605-12. 2002
    ..Inh2 associated with KPI-2 C-terminal domain with and without PP1C. Thus, KPI-2 is a kinase with sites to associate with PP1C and Inh2 to form a regulatory complex that is localized to membranes...
  12. ncbi request reprint Phosphorylation of phosphatase inhibitor-2 at centrosomes during mitosis
    Craig Leach
    Center for Cell Signaling, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
    J Biol Chem 278:26015-20. 2003
    ..Together, the data show dynamic changes in I-2 phosphorylation during mitosis and localization of phosphorylated I-2 at centrosomes, suggesting involvement in mammalian cell division...
  13. pmc Cytokine activation of p38 mitogen-activated protein kinase and apoptosis is opposed by alpha-4 targeting of protein phosphatase 2A for site-specific dephosphorylation of MEK3
    Todd D Prickett
    Center for Cell Signaling and Department of Microbiology, University of Virginia School of Medicine, Jefferson Park Avenue, Hospital West Complex, Room 7225, Charlottesville, VA 22908 1400, USA
    Mol Cell Biol 27:4217-27. 2007
    ..The results show that alpha-4 targets PP2A activity to MEK3 to suppress p38 MAPK activation by cytokines, thereby inhibiting apoptosis and anoikis...
  14. pmc Phosphatase inhibitor-2 balances protein phosphatase 1 and aurora B kinase for chromosome segregation and cytokinesis in human retinal epithelial cells
    Weiping Wang
    Center for Cell Signaling, Departments of Microbiology and Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    Mol Biol Cell 19:4852-62. 2008
    ..Conserved together throughout eukaryotic evolution, I-2, PP1 and Aurora B function interdependently during mitosis...
  15. ncbi request reprint Cellular chromium enhances activation of insulin receptor kinase
    Hong Wang
    Center for Cell Signaling, University of Virginia School of Medicine, Charlottesville, Virginia 22908 0577, USA
    Biochemistry 44:8167-75. 2005
    ..Our results suggest that nutritional and pharmacological therapies may complement one another to combat insulin resistance, a hallmark of type 2 diabetes...
  16. ncbi request reprint ATM-dependent dissociation of B55 regulatory subunit from nuclear PP2A in response to ionizing radiation
    Chang Y Guo
    Department of Radiation Oncology University of Virginia Health Science System, Charlottesville, Virginia 22908, USA
    J Biol Chem 277:4839-44. 2002
    ..We conclude a novel ATM-dependent mechanism is regulating association of B55 subunits with nuclear PP2A in response to IR...
  17. ncbi request reprint Overlapping binding sites in protein phosphatase 2A for association with regulatory A and alpha-4 (mTap42) subunits
    Todd D Prickett
    Center for Cell Signaling, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
    J Biol Chem 279:38912-20. 2004
    ..Our results reveal that the A subunit and alpha-4 (mTap42) require charged residues in separate but overlapping surface regions to associate with the back side of PP2Ac and modulate phosphatase activity...
  18. ncbi request reprint Protein phosphatase PP6 N terminal domain restricts G1 to S phase progression in human cancer cells
    Bjarki Stefansson
    Center for Cell Signaling, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
    Cell Cycle 6:1386-92. 2007
    ..Thus, our results provide evidence that PP6 regulates cell cycle progression in human cells at least in part through control of cyclin D1 and the function of PP6 is distinct from its homolog Sit4 in yeast...
  19. pmc Simian virus 40 small t antigen mediates conformation-dependent transfer of protein phosphatase 2A onto the androgen receptor
    Chun Song Yang
    Center for Cell Signaling, University of Virginia, Box 800577 Health Systems, Charlottesville, VA 22908, USA
    Mol Cell Biol 25:1298-308. 2005
    ..Thus, ST functions as a transfer factor to specify PP2A targeting in the cell and modulates the transcriptional activity of AR...
  20. ncbi request reprint Binding of phosphatase inhibitor-2 to prolyl isomerase Pin1 modifies specificity for mitotic phosphoproteins
    Mingguang Li
    Center for Cell Signaling, University of Virginia School of Medicine Charlottesville, Virginia 22908, USA
    Biochemistry 47:292-300. 2008
    ..The results reveal a novel function for I-2, and suggest phosphorylation-dependent regulation of Pin1 specificity during entry and exit of mitosis, in other phases of the cell cycle, and in multiple cell signaling processes...
  21. ncbi request reprint Migration and retraction of endothelial and epithelial cells require PHI-1, a specific protein-phosphatase-1 inhibitor protein
    Nikolaos A Tountas
    Center for Cell Signaling, University of Virginia School of Medicine, PO Box 800577, Charlottesville, VA 22908, USA
    J Cell Sci 117:5905-12. 2004
    ..Nonetheless, the results show that PHI-1 participates in regulatory events at the trailing edge of migrating cells and modulates retraction of endothelial and epithelial cells...
  22. ncbi request reprint Protein phosphatase-1alpha regulates centrosome splitting through Nek2
    Jun Mi
    Department of Radiation Oncology, University of Virginia Health System, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    Cancer Res 67:1082-9. 2007
    ..The identification of a PP1-specific isoform mediating a checkpoint response opens up the possibility of selectively targeting phosphatases as novel radiation sensitizers...
  23. ncbi request reprint Assembly of MYPT1 with protein phosphatase-1 in fibroblasts redirects localization and reorganizes the actin cytoskeleton
    Masumi Eto
    Department of Molecular Physiology and Biological Physics, Center for Cell Signaling, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
    Cell Motil Cytoskeleton 62:100-9. 2005
    ..Based on these responses, we conclude that the MYPT1 C-terminus functions as an auto-inhibitory domain, and a central domain in MYPT1 can mediate extensive reorganization of the actin cytoskeleton...
  24. ncbi request reprint The alpha4 regulatory subunit exerts opposing allosteric effects on protein phosphatases PP6 and PP2A
    Todd D Prickett
    Center for Cell Signaling and Department of Microbiology, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
    J Biol Chem 281:30503-11. 2006
    ..Transient expression of alpha4 differentially increased or decreased phosphorylation of endogenous phosphoproteins, consistent with opposing effects on PP2A and PP6...
  25. ncbi request reprint Juxtamembrane localization of the protein phosphatase-1 inhibitor protein PHI-1 in smooth muscle cells
    Nikolaos A Tountas
    Center for Cell Signaling, University of Virginia School of Medicine, PO Box 800577, Charlottesville, VA 22908, USA
    Histochem Cell Biol 121:343-50. 2004
    ..The common tissue distribution but different cellular localization of PHI-1 and CPI-17 suggest distinctive physiological roles for these two PP1 inhibitors...
  26. ncbi request reprint Phosphoprotein inhibitors of protein phosphatase-1
    Masumi Eto
    Center for Cell Signaling, Department of Molecular Physiology and Biological Physics, University of Virginia, School of Medicine, Charlottesville, Virginia 22908, USA
    Methods Enzymol 366:243-60. 2003
  27. pmc Ligand binding to the androgen receptor induces conformational changes that regulate phosphatase interactions
    Chun Song Yang
    Center for Cell Signaling, University of Virginia, Charlottesville, VA 22908, USA
    Mol Cell Biol 27:3390-404. 2007
    ..SV40 ST subverts this mechanism by promoting the direct transfer of PP2A onto androgen-bound AR, resulting in multisite dephosphorylation...
  28. pmc D-chiro-inositol glycans in insulin signaling and insulin resistance
    Joseph Larner
    Allomed Pharmaceuticals, Charlottesville, Virginia, United States of America
    Mol Med 16:543-52. 2010
    ..The metabolism of DCI is associated with insulin sensitivity and resistance, supporting the concept that second messengers have a role in responses to and resistance to insulin...
  29. ncbi request reprint Association of the tensin N-terminal protein-tyrosine phosphatase domain with the alpha isoform of protein phosphatase-1 in focal adhesions
    Masumi Eto
    Center for Cell Signaling, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
    J Biol Chem 282:17806-15. 2007
    ..Thus, the protein-tyrosine phosphatase domain of tensin exhibits isoform-specific association with PP1alpha in a restricted spatial region of adhesions that are formed during cell migration...
  30. ncbi request reprint Protein phosphatase 6 subunit with conserved Sit4-associated protein domain targets IkappaBepsilon
    Bjarki Stefansson
    Center for Cell Signaling, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
    J Biol Chem 281:22624-34. 2006
    ..The results show SAPS domain subunits recruit substrates such as IkappaBepsilon as one way to determine specific functions for PP6...
  31. ncbi request reprint Domains of type 1 protein phosphatase inhibitor-2 required for nuclear and cytoplasmic localization in response to cell-cell contact
    Craig Leach
    Center for Cell Signaling, University of Virginia School of Medicine, Box 800577 MSB7225, Charlottesville VA 22908, USA
    J Cell Sci 115:3739-45. 2002
    ..This change in inhibitor-2 localization may direct inhibitor-2 to different forms of protein phosphatase 1 or change the localization of protein phosphatase, as part of the cellular response to cell-cell contacts at high density...
  32. ncbi request reprint Inhibitor-2 regulates protein phosphatase-1 complexed with NimA-related kinase to induce centrosome separation
    Masumi Eto
    Center for Cell Signaling, University of Virginia School of Medicine, Charlottesville 22908, USA
    J Biol Chem 277:44013-20. 2002
    ..PP1C complex. Thus, Inh2 can enhance the kinase activity of the Nek2.PP1C complex via inhibition of phosphatase activity to initiate centrosome separation...
  33. ncbi request reprint Cerebellar long-term synaptic depression requires PKC-mediated activation of CPI-17, a myosin/moesin phosphatase inhibitor
    Masumi Eto
    Center for Cell Signaling, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    Neuron 36:1145-58. 2002
    ..Thus, a biochemical circuit that involves PKC-mediated activation of CPI-17 modulates the distinct physiological processes of vascular contractility and cerebellar LTD...
  34. ncbi request reprint Fluorescence lifetime imaging (FLIM) of green fluorescent fusion proteins in living cells
    Ammasi Periasamy
    Department of Biology, W M Keck Center for Cellular Imaging, University of Virginia, Charlottesville, VA, USA
    Methods Mol Biol 183:89-100. 2002
  35. ncbi request reprint Transgenic expression of protein phosphatase 2A regulatory subunit B56gamma disrupts distal lung differentiation
    Allen D Everett
    Department of Pediatrics, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
    Am J Physiol Lung Cell Mol Physiol 282:L1266-71. 2002
    ..We propose that B56gamma is expressed at a particular stage of lung development to modulate PP2A action on the Wnt/beta-catenin signaling pathway during lung airway morphogenesis...
  36. pmc Phosphoprotein inhibitor CPI-17 specificity depends on allosteric regulation of protein phosphatase-1 by regulatory subunits
    Masumi Eto
    Center for Cell Signaling, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    Proc Natl Acad Sci U S A 101:8888-93. 2004
    ..PP1C.P-CPI-17, leading to an increase in smooth muscle contraction...
  37. ncbi request reprint Developmental expression of alpha4 protein phosphatase regulatory subunit in tissues affected by Opitz syndrome
    Allen D Everett
    Department of Pediatrics and the Cardiovascular Research Center, University of Virginia Health System, Charlottesville, Virginia 22908 1356, USA
    Dev Dyn 224:461-4. 2002
    ..The alpha4 gene lies in the linkage interval for FG syndrome, characterized by skeletal muscle and brain defects that coincide closely to the expression pattern of alpha4...
  38. pmc Maternal phosphatase inhibitor-2 is required for proper chromosome segregation and mitotic synchrony during Drosophila embryogenesis
    Weiping Wang
    Center for Cell Signaling and Department of Microbiology, School of Medicine, University of Virginia, Charlottesville, Virginia 22908, USA
    Genetics 179:1823-33. 2008
    ..We propose that I-2 is required for proper chromosome segregation during Drosophila embryogenesis through the coordinated regulation of PP1 and Aurora B...
  39. pmc Midzone activation of aurora B in anaphase produces an intracellular phosphorylation gradient
    Brian G Fuller
    Department of Biochemistry, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
    Nature 453:1132-6. 2008
    ..We propose that feedback between aurora B kinase activation and midzone microtubules generates a gradient of post-translational marks that provides spatial information for events in anaphase and cytokinesis...
  40. ncbi request reprint Aurora-A kinase and inhibitor-2 regulate the cyclin threshold for mitotic entry in Xenopus early embryonic cell cycles
    David L Satinover
    Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
    Cell Cycle 5:2268-74. 2006
    ....
  41. ncbi request reprint Expression of ral GTPases, their effectors, and activators in human bladder cancer
    Steven Christopher Smith
    Department of Molecular Physiology, University of Virginia, Charlottesville, Virginia 22908, USA
    Clin Cancer Res 13:3803-13. 2007
    ....
  42. pmc TAB4 stimulates TAK1-TAB1 phosphorylation and binds polyubiquitin to direct signaling to NF-kappaB
    Todd D Prickett
    Center for Cell Signaling and Department of Microbiology, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
    J Biol Chem 283:19245-54. 2008
    ..The results show that TAB4 binds TAK1 and polyubiquitin chains to promote specific sites of phosphorylation in TAK1-TAB1, which activates IKK signaling to NF-kappaB...
  43. pmc Tensin1 requires protein phosphatase-1alpha in addition to RhoGAP DLC-1 to control cell polarization, migration, and invasion
    Emily H Hall
    Center for Cell Signaling and Department of Microbiology, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
    J Biol Chem 284:34713-22. 2009
    ..Our results show the importance of PP1alpha binding to tensin1 for the regulation of cell polarization, migration, and invasion...
  44. pmc Phosphorylation of RalB is important for bladder cancer cell growth and metastasis
    Hong Wang
    Department of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, Charlottesville, Virginia, USA
    Cancer Res 70:8760-9. 2010
    ..Our data show that RalA and RalB are phosphorylated by different kinases, and RalB phosphorylation is necessary for in vitro cellular functions and in vivo tumor growth and metastasis...
  45. doi request reprint Protein phosphatase 6 regulatory subunits composed of ankyrin repeat domains
    Bjarki Stefansson
    Center for Cell Signaling and Department of Microbiology, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
    Biochemistry 47:1442-51. 2008
    ..We propose that the SAPS and ankyrin repeat regulatory subunits determine the function and specificity of PP6...
  46. ncbi request reprint Phosphorylation and functions of inhibitor-2 family of proteins
    Mingguang Li
    Center for Cell Signaling and Department of Microbiology, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
    Biochemistry 46:2380-9. 2007
    ..The results show species specificity among the I-2 family within the context of conserved PP1 inhibitory activity and variable phosphorylation by Pro-directed kinases...
  47. ncbi request reprint Activation of glycogen synthase in myocardium induced by intermittent hypoxia is much lower in fasted than in fed rats
    Yangsong Wu
    Division of Endocrinology and Metabolism, Department of Internal Medicine, University of Virginia Health System, P O Box 801410, Charlottesville, VA 22908 1410, USA
    Am J Physiol Endocrinol Metab 292:E469-75. 2007
    ..We conclude that hypoxia activates GS in fed rat myocardium through a combination of rapid glycogenolysis, elevated local G-6-P content, and increased PP1 activity, and fasting attenuates this action independent of local G-6-P content...
  48. ncbi request reprint Peptide microarray analysis of substrate specificity of the transmembrane Ser/Thr kinase KPI-2 reveals reactivity with cystic fibrosis transmembrane conductance regulator and phosphorylase
    Hong Wang
    Center for Cell Signaling, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
    Mol Cell Proteomics 5:2124-30. 2006
    ..The results demonstrate the utility of the peptide library to probe specificity and discover kinase substrates and offer a specific assay that reveals hormonal regulation of the activity of this unusual transmembrane kinase...
  49. pmc Endotoxin conditioning induces VCP/p97-mediated and inducible nitric-oxide synthase-dependent Tyr284 nitration in protein phosphatase 2A
    Takashi Ohama
    Department of Microbiology, University of Virginia School of Medicine, Center for Cell Signaling, Charlottesville, Virginia 22908, USA
    J Biol Chem 285:8711-8. 2010
    ..We propose that VCP/p97-mediated Tyr nitration of PP2A increases the levels of phosphatases PP2A and DUSP1 to contribute to the refractory response of conditioned cells...
  50. ncbi request reprint Phosphorylation of the myosin-binding subunit of myosin phosphatase by Raf-1 and inhibition of phosphatase activity
    Constantinos G Broustas
    Department of Radiation Medicine, Lombardi Cancer Center, Georgetown University, Washington, DC 20007, USA
    J Biol Chem 277:3053-9. 2002
    ..Our studies identify MBS as a new substrate of Raf-1 and implicate a role for Raf-1 in the regulation of pathways involving myosin phosphatase activity...
  51. ncbi request reprint ATM regulates ionizing radiation-induced disruption of HDAC1:PP1:Rb complexes
    Changyue Guo
    Department of Radiation Oncology, University of Virginia Health System, Charlottesville, VA 22908, United States
    Cell Signal 19:504-10. 2007
    ..These results suggest that IR regulates HDAC1 phosphorylation and activity through ATM-dependent activation of PP1...
  52. ncbi request reprint Neurabins recruit protein phosphatase-1 and inhibitor-2 to the actin cytoskeleton
    Ryan T Terry-Lorenzo
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 277:46535-43. 2002
    ..I-2 complex to actin cytoskeleton and regulate cell morphology...
  53. ncbi request reprint The specificity of extracellular signal-regulated kinase 2 dephosphorylation by protein phosphatases
    Bo Zhou
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 277:31818-25. 2002
    ....
  54. pmc Targeting protein phosphatase 1 (PP1) to the actin cytoskeleton: the neurabin I/PP1 complex regulates cell morphology
    Carey J Oliver
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Mol Cell Biol 22:4690-701. 2002
    ..These studies provided new insights into the assembly and regulation of a neurabin I/PP1 complex that controls actin rearrangement to promote spine development in mammalian neurons...
  55. ncbi request reprint Phosphorylation of protein phosphatase type-1 inhibitory proteins by integrin-linked kinase and cyclic nucleotide-dependent protein kinases
    Ferenc Erdodi
    Department of Medical Chemistry, Medical and Health Science Center, University of Debrecen, H 4012 Debrecen, 18 B, Bem ter, Hungary
    Biochem Biophys Res Commun 306:382-7. 2003
    ..1 nM) and MPH (IC(50)=8 nM). These results suggest that the regulatory phosphorylation of CPI-17 and KEPI may involve distinct kinases and signaling pathways...
  56. pmc Protein phosphatase 6 down-regulates TAK1 kinase activation in the IL-1 signaling pathway
    Taisuke Kajino
    Department of Molecular Biology, Graduate School of Science, Nagoya University, Nagoya 464 8602, Japan
    J Biol Chem 281:39891-6. 2006
    ..Our results demonstrate that PP6 specifically down-regulates TAK1 through dephosphorylation of Thr-187 in the activation loop, which is likely important for suppressing inflammatory responses via TAK1 signaling pathways...
  57. pmc Phosphorylation of the myosin phosphatase inhibitors, CPI-17 and PHI-1, by integrin-linked kinase
    Jing Ti Deng
    Smooth Muscle Research Group and Canadian Institutes of Health Research Group in Regulation of Vascular Contractility, University of Calgary Faculty of Medicine, 3330 Hospital Drive N W, Calgary, Alberta T2N 4N1, Canada
    Biochem J 367:517-24. 2002
    ..We conclude that ILK may activate smooth-muscle contraction both directly, via phosphorylation of myosin, and indirectly, via phosphorylation and activation of CPI-17 and PHI-1, leading to inhibition of MLCP...
  58. ncbi request reprint Ionizing radiation activates nuclear protein phosphatase-1 by ATM-dependent dephosphorylation
    Chang Y Guo
    Department of Radiation Oncology, University of Virginia Health System, Charlottesville 22908, USA
    J Biol Chem 277:41756-61. 2002
    ..IR-induced PP1 activation in the nucleus may be a critical component in an ATM-mediated pathway controlling checkpoint activation...
  59. ncbi request reprint Distinctive solution conformation of phosphatase inhibitor CPI-17 substituted with aspartate at the phosphorylation-site threonine residue
    Shin ya Ohki
    CREST and Graduate School of Science, Tokyo Metropolitan University, 1 1 Minami Ohsawa, Hachioji, Tokyo 192 0397, Japan
    J Mol Biol 326:1539-47. 2003
    ..We propose that for this inhibitor, a negative charge at residue 38 is sufficient to trigger an active conformation, but a phosphoryl group is required for full inhibitory potency against protein phosphatase-1...
  60. ncbi request reprint The structure of Tap42/alpha4 reveals a tetratricopeptide repeat-like fold and provides insights into PP2A regulation
    Jing Yang
    Section of Structural Biology, Chester Beatty Laboratories, Institute of Cancer Research, 237 Fulham Road, London SW3 6JB, UK
    Biochemistry 46:8807-15. 2007
    ..We propose a scaffolding function for Tap42/alpha4 whereby the interaction of PP2Ac at its N-terminus promotes the dephosphorylation of substrates recruited to the C-terminal region of the molecule...
  61. pmc Subunit composition and developmental regulation of hepatic protein phosphatase 2A (PP2A)
    Sunny J S Yoo
    Department of Pediatrics, Rhode Island Hospital and Brown University, Providence, RI 02903, USA
    Arch Biochem Biophys 461:186-93. 2007
    ..In addition, a significant proportion of PP2A was in inactive forms that may involve novel regulatory subunits. Our results indicate that methylation of PP2A-C appears to be a primary determinant for the biogenesis of PP2A heterotrimers...
  62. pmc Phosphorylation-induced conformational switching of CPI-17 produces a potent myosin phosphatase inhibitor
    Masumi Eto
    Department of Molecular Physiology and Biophysics, Thomas Jefferson University, 1020 Locust Street, Philadelphia, PA 19107, USA
    Structure 15:1591-602. 2007
    ..Our results provide structural insights into transduction of kinase signals by PP1 inhibitor proteins...
  63. pmc Differential signalling by muscarinic receptors in smooth muscle: m2-mediated inactivation of myosin light chain kinase via Gi3, Cdc42/Rac1 and p21-activated kinase 1 pathway, and m3-mediated MLC20 (20 kDa regulatory light chain of myosin II) phosphorylat
    Karnam S Murthy
    Departments of Physiology and Medicine, Medical College of Virginia, Virginia Commonwealth University, Richmond, VA 23298, USA
    Biochem J 374:145-55. 2003
    ..m2-dependent phosphorylation and inactivation of MLCK precluded its involvement in sustained MLC20 phosphorylation and contraction...
  64. ncbi request reprint Assay for three-way interaction of protein phosphatase-1 (Glc7) with regulatory subunits plus phosphatase inhibitor-2
    Masumi Eto
    Department of Physiology, Thomas Jefferson College of Medicine, Philadelphia, PA, USA
    Methods Mol Biol 365:197-208. 2007
    ..Mutations in the canonical recognition motif VxF used to bind PP1 (Glc7) allow for analysis of direct from indirect (three-way) interactions...
  65. ncbi request reprint Proper chromatin condensation and maintenance of histone H3 phosphorylation during mouse oocyte meiosis requires protein phosphatase activity
    Jason E Swain
    Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, MI 48109 0617, USA
    Biol Reprod 76:628-38. 2007
    ..Furthermore, GSK3 inhibition results in aberrant chromatin condensation and appears to regulate phosphorylation of PPP1R2...
  66. ncbi request reprint Phosphorylation of the Pro-X-Thr-Pro site in phosphatase inhibitor-2 by cyclin-dependent protein kinase during M-phase of the cell cycle
    Mingguang Li
    Center for Cell Signaling and Department of Microbiology University of Virginia School of Medicine Charlottesville, VA 22908, USA
    Cell Signal 18:1318-26. 2006
    ..Thus, phosphorylation of I-2 is catalyzed by cdk1::cyclinB1 and staining with a specific antibody should prove useful as a selective marker of cells in the early stages of mitosis...
  67. pmc The alpha4-containing form of protein phosphatase 2A in liver and hepatic cells
    Sunny J S Yoo
    Department of Pediatrics, Rhode Island Hospital and Brown University, Providence, Rhode Island 02903, USA
    J Cell Biochem 105:290-300. 2008
    ..Our results indicate that the yeast Tap42/TOR paradigm is not conserved in hepatic cells...

Research Grants29

  1. CHROMIUM ENHANCEMENT OF INSULIN SIGNALING
    DAVID BRAUTIGAN; Fiscal Year: 2005
    ..abstract_text> ..
  2. Phosphorylation and Function of Inhibitor-2
    DAVID BRAUTIGAN; Fiscal Year: 2005
    ..The results of this research will provide new fundamental knowledge of mechanisms controlling centrosomes and cell cycle progression that is relevant to understanding human disease. ..
  3. RAPAMYCIN BLOCKADE OF PROTEIN PHOSPHATASE SIGNALING
    DAVID BRAUTIGAN; Fiscal Year: 2005
    ..The proposed studies will give new insights into an under-studied phosphatase-signaling pathway and provide information on the molecular basis for action of and resistance to rapamycin. ..
  4. CHROMIUM ENHANCEMENT OF INSULIN SIGNALING
    DAVID BRAUTIGAN; Fiscal Year: 2006
    ..abstract_text> ..
  5. RAPAMYCIN BLOCKADE OF PROTEIN PHOSPHATASE SIGNALING
    DAVID BRAUTIGAN; Fiscal Year: 2006
    ..The proposed studies will give new insights into an under-studied phosphatase-signaling pathway and provide information on the molecular basis for action of and resistance to rapamycin. ..
  6. RAPAMYCIN BLOCKADE OF PROTEIN PHOSPHATASE SIGNALING
    DAVID BRAUTIGAN; Fiscal Year: 2007
    ..The proposed studies will give new insights into an under-studied phosphatase-signaling pathway and provide information on the molecular basis for action of and resistance to rapamycin. ..
  7. CORE--MICROSCOPY
    DAVID BRAUTIGAN; Fiscal Year: 2007
    ..abstract_text> ..
  8. Phosphorylation & Function of Inhibitor-2
    DAVID BRAUTIGAN; Fiscal Year: 2009
    ..The goal is to understand how this protein acts to connect multiple signaling inputs into centrosome actions. Understanding cellular mechanisms is the basis for diagnosis and treatment of human diseases. ..
  9. Phosphorylation & Function of Inhibitor-2
    DAVID BRAUTIGAN; Fiscal Year: 2009
    ..The goal is to understand how this protein acts to connect multiple signaling inputs into centrosome actions. Understanding cellular mechanisms is the basis for diagnosis and treatment of human diseases. ..
  10. RAPAMYCIN BLOCKADE OF PROTEIN PHOSPHATASE SIGNALING
    DAVID BRAUTIGAN; Fiscal Year: 2004
    ..The proposed studies will give new insights into an under-studied phosphatase-signaling pathway and provide information on the molecular basis for action of and resistance to rapamycin. ..
  11. CHROMIUM ENHANCEMENT OF INSULIN SIGNALING
    DAVID BRAUTIGAN; Fiscal Year: 2004
    ..abstract_text> ..
  12. Phosphorylation and Function of Inhibitor-2
    DAVID BRAUTIGAN; Fiscal Year: 2004
    ..The results of this research will provide new fundamental knowledge of mechanisms controlling centrosomes and cell cycle progression that is relevant to understanding human disease. ..
  13. RAPAMYCIN BLOCKADE OF PROTEIN PHOSPHATASE SIGNALING
    DAVID BRAUTIGAN; Fiscal Year: 1999
    ..This project will discover new molecular mechanisms for control of protein synthesis and cell growth that are sensitive to rapamycin. ..
  14. PHOSPHORYLATION AND FUNCTION OF INHIBITOR 2
    DAVID BRAUTIGAN; Fiscal Year: 1999
    ..Together, these studies will elucidate new roles for Inh2 and PP1 in growth control, and expose the potential of defects in these proteins in human cancers. ..
  15. RAPAMYCIN BLOCKADE OF PROTEIN PHOSPHATASE SIGNALING
    DAVID BRAUTIGAN; Fiscal Year: 2000
    ..This project will discover new molecular mechanisms for control of protein synthesis and cell growth that are sensitive to rapamycin. ..
  16. PHOSPHORYLATION AND FUNCTION OF INHIBITOR 2
    DAVID BRAUTIGAN; Fiscal Year: 2000
    ..Together, these studies will elucidate new roles for Inh2 and PP1 in growth control, and expose the potential of defects in these proteins in human cancers. ..
  17. RAPAMYCIN BLOCKADE OF PROTEIN PHOSPHATASE SIGNALING
    DAVID BRAUTIGAN; Fiscal Year: 2001
    ..This project will discover new molecular mechanisms for control of protein synthesis and cell growth that are sensitive to rapamycin. ..
  18. RAPAMYCIN BLOCKADE OF PROTEIN PHOSPHATASE SIGNALING
    DAVID BRAUTIGAN; Fiscal Year: 2002
    ..This project will discover new molecular mechanisms for control of protein synthesis and cell growth that are sensitive to rapamycin. ..
  19. Phosphorylation and Function of Inhibitor-2
    DAVID BRAUTIGAN; Fiscal Year: 2002
    ..The results of this research will provide new fundamental knowledge of mechanisms controlling centrosomes and cell cycle progression that is relevant to understanding human disease. ..
  20. CHROMIUM ENHANCEMENT OF INSULIN SIGNALING
    DAVID BRAUTIGAN; Fiscal Year: 2002
    ..abstract_text> ..
  21. Phosphorylation and Function of Inhibitor-2
    DAVID BRAUTIGAN; Fiscal Year: 2003
    ..The results of this research will provide new fundamental knowledge of mechanisms controlling centrosomes and cell cycle progression that is relevant to understanding human disease. ..
  22. CHROMIUM ENHANCEMENT OF INSULIN SIGNALING
    DAVID BRAUTIGAN; Fiscal Year: 2003
    ..abstract_text> ..
  23. RAPAMYCIN BLOCKADE OF PROTEIN PHOSPHATASE SIGNALING
    DAVID BRAUTIGAN; Fiscal Year: 2003
    ..The proposed studies will give new insights into an under-studied phosphatase-signaling pathway and provide information on the molecular basis for action of and resistance to rapamycin. ..
  24. Phosphorylation & Function of Inhibitor-2
    David L Brautigan; Fiscal Year: 2010
    ..The goal is to understand how this protein acts to connect multiple signaling inputs into centrosome actions. Understanding cellular mechanisms is the basis for diagnosis and treatment of human diseases. ..