David A Brafman

Summary

Affiliation: University of California
Country: USA

Publications

  1. doi request reprint Generation, Expansion, and Differentiation of Human Pluripotent Stem Cell (hPSC) Derived Neural Progenitor Cells (NPCs)
    David A Brafman
    Department of Cellular and Molecular Medicine, Stem Cell Program, UCSD, 9500 Gilman Drive, La Jolla, CA, 92093 0395, USA
    Methods Mol Biol 1212:87-102. 2015
  2. pmc Regulation of endodermal differentiation of human embryonic stem cells through integrin-ECM interactions
    D A Brafman
    Department of Cellular and Molecular Medicine, Stem Cell Program, University of California, La Jolla, CA 92093, USA
    Cell Death Differ 20:369-81. 2013
  3. doi request reprint Investigating the role of the extracellular environment in modulating hepatic stellate cell biology with arrayed combinatorial microenvironments
    David A Brafman
    Department of Bioengineering, University of California, San Diego, USA
    Integr Biol (Camb) 1:513-24. 2009
  4. doi request reprint Arrayed cellular microenvironments for identifying culture and differentiation conditions for stem, primary and rare cell populations
    David A Brafman
    Cellular and Molecular Medicine, Stem Cell Program, University of California, San Diego, California, USA
    Nat Protoc 7:703-17. 2012
  5. doi request reprint Defining long-term maintenance conditions of human embryonic stem cells with arrayed cellular microenvironment technology
    David A Brafman
    Department of Bioengineering, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0412, USA
    Stem Cells Dev 18:1141-54. 2009
  6. pmc Long-term human pluripotent stem cell self-renewal on synthetic polymer surfaces
    David A Brafman
    Department of Bioengineering, University of California San Diego, 9500 Gilman Dr, La Jolla, CA 92093 0695, United States
    Biomaterials 31:9135-44. 2010
  7. pmc Endogenous WNT Signaling Regulates hPSC-Derived Neural Progenitor Cell Heterogeneity and Specifies Their Regional Identity
    Noel Moya
    Department of Cellular and Molecular Medicine, Stem Cell Program, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0695, USA
    Stem Cell Reports 3:1015-28. 2014
  8. pmc Analysis of SOX2-expressing cell populations derived from human pluripotent stem cells
    David A Brafman
    Stem Cell Program, Department of Cellular and Molecular Medicine, UCSD, 9500 Gilman Drive, La Jolla, CA 92093 0695, USA
    Stem Cell Reports 1:464-78. 2013
  9. doi request reprint Constructing stem cell microenvironments using bioengineering approaches
    David A Brafman
    Department of Cellular and Molecular Medicine, Stem Cell Program, University of California at San Diego, La Jolla, California
    Physiol Genomics 45:1123-35. 2013

Collaborators

Detail Information

Publications9

  1. doi request reprint Generation, Expansion, and Differentiation of Human Pluripotent Stem Cell (hPSC) Derived Neural Progenitor Cells (NPCs)
    David A Brafman
    Department of Cellular and Molecular Medicine, Stem Cell Program, UCSD, 9500 Gilman Drive, La Jolla, CA, 92093 0395, USA
    Methods Mol Biol 1212:87-102. 2015
    ..Finally, this protocol allows for the robust differentiation of NPCs into microtubule-associated protein 2 (MAP2) and β-Tubulin-III (β3T) positive neurons...
  2. pmc Regulation of endodermal differentiation of human embryonic stem cells through integrin-ECM interactions
    D A Brafman
    Department of Cellular and Molecular Medicine, Stem Cell Program, University of California, La Jolla, CA 92093, USA
    Cell Death Differ 20:369-81. 2013
    ..These data provide evidence that FN and VTN promote endoderm differentiation of hESCs through interaction with ITGA5 and ITGAV, and that ECMP-integrin interactions are required for hESC differentiation into functionally mature cells...
  3. doi request reprint Investigating the role of the extracellular environment in modulating hepatic stellate cell biology with arrayed combinatorial microenvironments
    David A Brafman
    Department of Bioengineering, University of California, San Diego, USA
    Integr Biol (Camb) 1:513-24. 2009
    ..Our results indicate that array-based screens may provide an efficient means for identifying candidate signaling pathways to be targeted for anti-fibrotic therapies...
  4. doi request reprint Arrayed cellular microenvironments for identifying culture and differentiation conditions for stem, primary and rare cell populations
    David A Brafman
    Cellular and Molecular Medicine, Stem Cell Program, University of California, San Diego, California, USA
    Nat Protoc 7:703-17. 2012
    ..A standard ACME screen uses the technologies previously applied to the manufacture and analysis of DNA microarrays, requires standard cell-culture facilities and can be performed from beginning to end within 5-10 days...
  5. doi request reprint Defining long-term maintenance conditions of human embryonic stem cells with arrayed cellular microenvironment technology
    David A Brafman
    Department of Bioengineering, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0412, USA
    Stem Cells Dev 18:1141-54. 2009
    ..In the future, the novel array platform and analysis procedure presented here will be applied toward the directed differentiation of hESCs and maintenance of other stem and progenitor cell populations...
  6. pmc Long-term human pluripotent stem cell self-renewal on synthetic polymer surfaces
    David A Brafman
    Department of Bioengineering, University of California San Diego, 9500 Gilman Dr, La Jolla, CA 92093 0695, United States
    Biomaterials 31:9135-44. 2010
    ....
  7. pmc Endogenous WNT Signaling Regulates hPSC-Derived Neural Progenitor Cell Heterogeneity and Specifies Their Regional Identity
    Noel Moya
    Department of Cellular and Molecular Medicine, Stem Cell Program, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0695, USA
    Stem Cell Reports 3:1015-28. 2014
    ..The ability to manipulate WNT signaling to generate regionally specific NPCs and neurons will be useful for studying human neural development and will greatly enhance the translational potential of hPSCs for neural-related therapies. ..
  8. pmc Analysis of SOX2-expressing cell populations derived from human pluripotent stem cells
    David A Brafman
    Stem Cell Program, Department of Cellular and Molecular Medicine, UCSD, 9500 Gilman Drive, La Jolla, CA 92093 0695, USA
    Stem Cell Reports 1:464-78. 2013
    ..In AFE, we used transcriptome-wide expression analysis to identify cell surface markers with elevated expression in this population, thereby facilitating isolation and purification of this hPSC-derived cell population. ..
  9. doi request reprint Constructing stem cell microenvironments using bioengineering approaches
    David A Brafman
    Department of Cellular and Molecular Medicine, Stem Cell Program, University of California at San Diego, La Jolla, California
    Physiol Genomics 45:1123-35. 2013
    ..Finally, the emerging trend of using high-throughput, combinatorial methods for the stepwise engineering of stem cell microenvironments will be explored. ..