David A Brafman
Affiliation: University of California
- Regulation of endodermal differentiation of human embryonic stem cells through integrin-ECM interactionsD A Brafman
Department of Cellular and Molecular Medicine, Stem Cell Program, University of California, La Jolla, CA 92093, USA
Cell Death Differ 20:369-81. 2013..These data provide evidence that FN and VTN promote endoderm differentiation of hESCs through interaction with ITGA5 and ITGAV, and that ECMP-integrin interactions are required for hESC differentiation into functionally mature cells...
- Investigating the role of the extracellular environment in modulating hepatic stellate cell biology with arrayed combinatorial microenvironmentsDavid A Brafman
Department of Bioengineering, University of California, San Diego, USA
Integr Biol (Camb) 1:513-24. 2009..Our results indicate that array-based screens may provide an efficient means for identifying candidate signaling pathways to be targeted for anti-fibrotic therapies...
- Arrayed cellular microenvironments for identifying culture and differentiation conditions for stem, primary and rare cell populationsDavid A Brafman
Cellular and Molecular Medicine, Stem Cell Program, University of California, San Diego, California, USA
Nat Protoc 7:703-17. 2012..A standard ACME screen uses the technologies previously applied to the manufacture and analysis of DNA microarrays, requires standard cell-culture facilities and can be performed from beginning to end within 5-10 days...
- Defining long-term maintenance conditions of human embryonic stem cells with arrayed cellular microenvironment technologyDavid A Brafman
Department of Bioengineering, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0412, USA
Stem Cells Dev 18:1141-54. 2009..In the future, the novel array platform and analysis procedure presented here will be applied toward the directed differentiation of hESCs and maintenance of other stem and progenitor cell populations...
- Long-term human pluripotent stem cell self-renewal on synthetic polymer surfacesDavid A Brafman
Department of Bioengineering, University of California San Diego, 9500 Gilman Dr, La Jolla, CA 92093 0695, United States
Biomaterials 31:9135-44. 2010....
- Constructing stem cell microenvironments using bioengineering approachesDavid A Brafman
Department of Cellular and Molecular Medicine, Stem Cell Program, University of California at San Diego, La Jolla, California
Physiol Genomics 45:1123-35. 2013..Finally, the emerging trend of using high-throughput, combinatorial methods for the stepwise engineering of stem cell microenvironments will be explored. ..