N A Bradbury

Summary

Affiliation: University of Pittsburgh
Country: USA

Publications

  1. ncbi request reprint Characterization of the internalization pathways for the cystic fibrosis transmembrane conductance regulator
    N A Bradbury
    Cystic Fibrosis Research Center, Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    Am J Physiol 276:L659-68. 1999
  2. ncbi request reprint Mu 2 binding directs the cystic fibrosis transmembrane conductance regulator to the clathrin-mediated endocytic pathway
    K M Weixel
    Cystic Fibrosis Research Center, Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 276:46251-9. 2001
  3. ncbi request reprint Trafficking of the Ca2+-activated K+ channel, hIK1, is dependent upon a C-terminal leucine zipper
    Colin A Syme
    Department of Cell Biology and Physiology, University of Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 278:8476-86. 2003
  4. ncbi request reprint Analysis of CFTR endocytosis by cell surface biotinylation
    Kelly Weixel
    Cystic Fibrosis Research Centre, Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
    Methods Mol Med 70:323-40. 2002
  5. ncbi request reprint Role of the NH2 terminus in the assembly and trafficking of the intermediate conductance Ca2+-activated K+ channel hIK1
    Heather M Jones
    Department of Cell Biology and Physiology, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 279:15531-40. 2004
  6. ncbi request reprint Endocytic adaptor complexes bind the C-terminal domain of CFTR
    K M Weixel
    Cystic Fibrosis Research Centre, S306 BST South, Department of Cell Biology and Physiology, 3500 Terrace Street, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    Pflugers Arch 443:S70-4. 2001
  7. pmc p27Kip1 localizes to detergent-insoluble microdomains within lymphocyte membranes
    B B Yaroslavskiy
    Department of Medicine, University of Pittsburgh School of Medicine, Pennsylvania 15213, USA
    Mol Med 7:49-58. 2001
  8. ncbi request reprint E3KARP mediates the association of ezrin and protein kinase A with the cystic fibrosis transmembrane conductance regulator in airway cells
    F Sun
    Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 275:29539-46. 2000
  9. ncbi request reprint Protein kinase A associates with cystic fibrosis transmembrane conductance regulator via an interaction with ezrin
    F Sun
    Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 275:14360-6. 2000
  10. pmc Exocyst requirement for endocytic traffic directed toward the apical and basolateral poles of polarized MDCK cells
    Asli Oztan
    Laboratory of Epithelial Cell Biology Renal Electrolyte Division of the Department of Medicine, University of Pittsburgh, Pittsburgh, PA 15261, USA
    Mol Biol Cell 18:3978-92. 2007

Collaborators

Detail Information

Publications16

  1. ncbi request reprint Characterization of the internalization pathways for the cystic fibrosis transmembrane conductance regulator
    N A Bradbury
    Cystic Fibrosis Research Center, Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    Am J Physiol 276:L659-68. 1999
    ..These results suggest that CFTR is constitutively internalized from the apical plasma membrane via clathrin-coated pits and that CFTR is excluded from caveolae...
  2. ncbi request reprint Mu 2 binding directs the cystic fibrosis transmembrane conductance regulator to the clathrin-mediated endocytic pathway
    K M Weixel
    Cystic Fibrosis Research Center, Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 276:46251-9. 2001
    ..Furthermore, this interaction is mediated by the mu 2 subunit of AP-2 and mutations in mu 2 that block its interaction with YDSI inhibit the incorporation of CFTR into the clathrin-mediated endocytic pathway...
  3. ncbi request reprint Trafficking of the Ca2+-activated K+ channel, hIK1, is dependent upon a C-terminal leucine zipper
    Colin A Syme
    Department of Cell Biology and Physiology, University of Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 278:8476-86. 2003
    ..In conclusion, we demonstrate that the C-terminal leucine zipper is critical to facilitate correct folding and plasma membrane trafficking of hIK1, whereas this function is not conserved in other gene family members...
  4. ncbi request reprint Analysis of CFTR endocytosis by cell surface biotinylation
    Kelly Weixel
    Cystic Fibrosis Research Centre, Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
    Methods Mol Med 70:323-40. 2002
  5. ncbi request reprint Role of the NH2 terminus in the assembly and trafficking of the intermediate conductance Ca2+-activated K+ channel hIK1
    Heather M Jones
    Department of Cell Biology and Physiology, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 279:15531-40. 2004
    ..In conclusion, we demonstrate that the NH(2) terminus of hIK1 contains overlapping leucine zipper and dileucine motifs essential for channel assembly and trafficking to the plasma membrane...
  6. ncbi request reprint Endocytic adaptor complexes bind the C-terminal domain of CFTR
    K M Weixel
    Cystic Fibrosis Research Centre, S306 BST South, Department of Cell Biology and Physiology, 3500 Terrace Street, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    Pflugers Arch 443:S70-4. 2001
    ..Thus, we demonstrate that CFTR is endocytosed via clathrin-coated vesicles, and that targeting of CFTR to these structures is mediated by binding of the AP-2 adaptor complex to the C-terminal domain of CFTR...
  7. pmc p27Kip1 localizes to detergent-insoluble microdomains within lymphocyte membranes
    B B Yaroslavskiy
    Department of Medicine, University of Pittsburgh School of Medicine, Pennsylvania 15213, USA
    Mol Med 7:49-58. 2001
    ..Because some cell surface signaling complexes lose p27Kip1 upon cellular activation, p27Kip1 may play a functional role in modulating membrane signaling...
  8. ncbi request reprint E3KARP mediates the association of ezrin and protein kinase A with the cystic fibrosis transmembrane conductance regulator in airway cells
    F Sun
    Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 275:29539-46. 2000
    ..Since ezrin is also an actin-binding protein, the formation of a CFTR.E3KARP.ezrin complex may be important also in stabilizing CFTR at the apical membrane domain of airway cells...
  9. ncbi request reprint Protein kinase A associates with cystic fibrosis transmembrane conductance regulator via an interaction with ezrin
    F Sun
    Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 275:14360-6. 2000
    ..Taken together, these data demonstrate that PKA II is linked physically and functionally to CFTR by an AKAP interaction, and they suggest that ezrin serves as an AKAP for PKA-mediated phosphorylation of CFTR...
  10. pmc Exocyst requirement for endocytic traffic directed toward the apical and basolateral poles of polarized MDCK cells
    Asli Oztan
    Laboratory of Epithelial Cell Biology Renal Electrolyte Division of the Department of Medicine, University of Pittsburgh, Pittsburgh, PA 15261, USA
    Mol Biol Cell 18:3978-92. 2007
    ....
  11. ncbi request reprint Protein kinase-A-mediated secretion of mucin from human colonic epithelial cells
    N A Bradbury
    Cystic Fibrosis Research Center, Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    J Cell Physiol 185:408-15. 2000
    ..Subsequent addition of forskolin was unable to further increase mucin secretion. Thus, activation of either type I or type II PKA is able to maximally stimulate secretion of mucins from T84 human colonic epithelial cells...
  12. ncbi request reprint cAMP signaling cascades and CFTR: is there more to learn?
    N A Bradbury
    Cystic Fibrosis Research Centre, Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh PA 15261, USA
    Pflugers Arch 443:S85-91. 2001
    ..The intent of this review is to evaluate our current understanding of the cAMP mediated signaling as it relates to the secretion of mucin and chloride, two compounds whose regulated secretion is altered in cystic fibrosis...
  13. ncbi request reprint Rme-1 regulates the recycling of the cystic fibrosis transmembrane conductance regulator
    John A Picciano
    Cystic Fibrosis Research Center, Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, 3500 Terrace Street, Pittsburgh, PA 15261, USA
    Am J Physiol Cell Physiol 285:C1009-18. 2003
    ....
  14. ncbi request reprint A mutation in the cystic fibrosis transmembrane conductance regulator generates a novel internalization sequence and enhances endocytic rates
    Mark R Silvis
    Cystic Fibrosis Research Center, Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 278:11554-60. 2003
    ....
  15. pmc Rab11b regulates the apical recycling of the cystic fibrosis transmembrane conductance regulator in polarized intestinal epithelial cells
    Mark R Silvis
    Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, PA 15261, USA
    Mol Biol Cell 20:2337-50. 2009
    ..This is the first report detailing apical CFTR recycling in a native expression system and to demonstrate that Rab11b regulates apical recycling in polarized epithelial cells...
  16. pmc Endocytic trafficking of CFTR in health and disease
    Nadia Ameen
    Department of Paediatrics, University of Pittsburgh School of Medicine, PA, USA
    J Cyst Fibros 6:1-14. 2007
    ....