E Fidelma Boyd

Summary

Affiliation: University of Delaware
Country: USA

Publications

  1. pmc Sialic acid catabolism and transport gene clusters are lineage specific in Vibrio vulnificus
    Jean Bernard Lubin
    Microbiology Research Group, Institute of Integrative Biology, University of Liverpool, Liverpool, United Kingdom
    Appl Environ Microbiol 78:3407-15. 2012
  2. doi request reprint Genomic islands are dynamic, ancient integrative elements in bacterial evolution
    E Fidelma Boyd
    Department of Biological Sciences, University of Delaware, Newark, DE 19716, USA
    Trends Microbiol 17:47-53. 2009
  3. pmc Rapid multiplex PCR and real-time TaqMan PCR assays for detection of Salmonella enterica and the highly virulent serovars Choleraesuis and Paratyphi C
    David F Woods
    Department of Biological Sciences, University of Delaware, Newark, DE 19716
    J Clin Microbiol 46:4018-22. 2008
  4. pmc Molecular analysis of the emergence of pandemic Vibrio parahaemolyticus
    E Fidelma Boyd
    Department of Biological Sciences, University of Delaware, Newark, DE 19716, USA
    BMC Microbiol 8:110. 2008
  5. pmc Insights into the evolution of sialic acid catabolism among bacteria
    Salvador Almagro-Moreno
    Department of Biological Sciences, University of Delaware, Newark, DE 19716, USA
    BMC Evol Biol 9:118. 2009
  6. pmc The Vibrio parahaemolyticus ToxRS regulator is required for stress tolerance and colonization in a novel orogastric streptomycin-induced adult murine model
    W Brian Whitaker
    Department of Biological Sciences, University of Delaware, Newark, Delaware, USA
    Infect Immun 80:1834-45. 2012
  7. pmc Modulation of responses of Vibrio parahaemolyticus O3:K6 to pH and temperature stresses by growth at different salt concentrations
    W Brian Whitaker
    Department of Biological Sciences, University of Delaware, Newark, DE 19711, USA
    Appl Environ Microbiol 76:4720-9. 2010
  8. doi request reprint Dichotomy in the evolution of pathogenicity island and bacteriophage encoded integrases from pathogenic Escherichia coli strains
    Michael G Napolitano
    Department of Biological Sciences, University of Delaware, Newark, DE 19716, USA
    Infect Genet Evol 11:423-36. 2011
  9. pmc Excision dynamics of Vibrio pathogenicity island-2 from Vibrio cholerae: role of a recombination directionality factor VefA
    Salvador Almagro-Moreno
    Department of Biological Sciences, University of Delaware, Newark, DE 19716, USA
    BMC Microbiol 10:306. 2010
  10. doi request reprint The VC1777-VC1779 proteins are members of a sialic acid-specific subfamily of TRAP transporters (SiaPQM) and constitute the sole route of sialic acid uptake in the human pathogen Vibrio cholerae
    Nityananda Chowdhury
    Department of Biological Sciences, University of Delaware, Newark, DE 19716, USA
    Microbiology 158:2158-67. 2012

Collaborators

Detail Information

Publications31

  1. pmc Sialic acid catabolism and transport gene clusters are lineage specific in Vibrio vulnificus
    Jean Bernard Lubin
    Microbiology Research Group, Institute of Integrative Biology, University of Liverpool, Liverpool, United Kingdom
    Appl Environ Microbiol 78:3407-15. 2012
    ..Overall, our study demonstrates that the ability to catabolize and transport sialic acid is predominately lineage specific in V. vulnificus and that the TRAP transporter is essential for sialic acid uptake...
  2. doi request reprint Genomic islands are dynamic, ancient integrative elements in bacterial evolution
    E Fidelma Boyd
    Department of Biological Sciences, University of Delaware, Newark, DE 19716, USA
    Trends Microbiol 17:47-53. 2009
    ..In addition, we explore the diversity of genomic islands and their insertion sites among Gram-negative bacteria and discuss why they integrate at a limited number of tRNA genes...
  3. pmc Rapid multiplex PCR and real-time TaqMan PCR assays for detection of Salmonella enterica and the highly virulent serovars Choleraesuis and Paratyphi C
    David F Woods
    Department of Biological Sciences, University of Delaware, Newark, DE 19716
    J Clin Microbiol 46:4018-22. 2008
    ..The technique was also adapted for a real-time PCR rapid-detection assay for both serovars Choleraesuis and Paratyphi C that complements the current procedures for Salmonella sp. isolation and serotyping...
  4. pmc Molecular analysis of the emergence of pandemic Vibrio parahaemolyticus
    E Fidelma Boyd
    Department of Biological Sciences, University of Delaware, Newark, DE 19716, USA
    BMC Microbiol 8:110. 2008
    ..This new highly virulent strain is now globally disseminated...
  5. pmc Insights into the evolution of sialic acid catabolism among bacteria
    Salvador Almagro-Moreno
    Department of Biological Sciences, University of Delaware, Newark, DE 19716, USA
    BMC Evol Biol 9:118. 2009
    ..Sialic acids from the human host are used by a number of pathogens as an energy source. Here we explore the evolution of the genes involved in the catabolism of sialic acid...
  6. pmc The Vibrio parahaemolyticus ToxRS regulator is required for stress tolerance and colonization in a novel orogastric streptomycin-induced adult murine model
    W Brian Whitaker
    Department of Biological Sciences, University of Delaware, Newark, Delaware, USA
    Infect Immun 80:1834-45. 2012
    ..Thus, the defect in stress tolerance and colonization in ΔtoxRS is solely due to OmpU. To our knowledge, the orogastric adult murine model reported here is the first showing sustained intestinal colonization by V. parahaemolyticus...
  7. pmc Modulation of responses of Vibrio parahaemolyticus O3:K6 to pH and temperature stresses by growth at different salt concentrations
    W Brian Whitaker
    Department of Biological Sciences, University of Delaware, Newark, DE 19711, USA
    Appl Environ Microbiol 76:4720-9. 2010
    ..The effect of V. parahaemolyticus growth in 1% or 3% NaCl was examined using a cytotoxicity assay, and we found that V. parahaemolyticus grown in 1% NaCl was significantly more toxic than that grown in 3% NaCl...
  8. doi request reprint Dichotomy in the evolution of pathogenicity island and bacteriophage encoded integrases from pathogenic Escherichia coli strains
    Michael G Napolitano
    Department of Biological Sciences, University of Delaware, Newark, DE 19716, USA
    Infect Genet Evol 11:423-36. 2011
    ..coli PAIs. Overall this work is the first to demonstrate a dichotomy in the evolution of integrases encoded on PAIs and phages from pathogenic E. coli suggesting that PAIs are an evolutionary distinct genetic element...
  9. pmc Excision dynamics of Vibrio pathogenicity island-2 from Vibrio cholerae: role of a recombination directionality factor VefA
    Salvador Almagro-Moreno
    Department of Biological Sciences, University of Delaware, Newark, DE 19716, USA
    BMC Microbiol 10:306. 2010
    ..IntV2 is required for VPI-2 excision from chromosome 1, which occurs at very low levels, and formation of a non-replicative circular intermediate...
  10. doi request reprint The VC1777-VC1779 proteins are members of a sialic acid-specific subfamily of TRAP transporters (SiaPQM) and constitute the sole route of sialic acid uptake in the human pathogen Vibrio cholerae
    Nityananda Chowdhury
    Department of Biological Sciences, University of Delaware, Newark, DE 19716, USA
    Microbiology 158:2158-67. 2012
    ..Together these data demonstrate unequivocally that the siaPQM-encoded TRAP transporter within VPI-2 is the sole sialic acid transporter in V. cholerae...
  11. pmc Osmoadaptation among Vibrio species and unique genomic features and physiological responses of Vibrio parahaemolyticus
    Lynn M Naughton
    Department of Biological Sciences, University of Delaware, Newark, DE 19716, USA
    Appl Environ Microbiol 75:2802-10. 2009
    ..We complemented E. coli MKH13, a compatible solute transporter-negative strain, with bcct2 and demonstrated uptake of betaine at high salt concentrations...
  12. pmc Genomic and metabolic profiling of nonulosonic acids in Vibrionaceae reveal biochemical phenotypes of allelic divergence in Vibrio vulnificus
    Amanda L Lewis
    Department of Molecular Microbiology, Washington University, St Louis, Missouri 63110, USA
    Appl Environ Microbiol 77:5782-93. 2011
    ..Taken together, the data implicate the family Vibrionaceae as a "hot spot" of NulO evolution and suggest that these molecules may have diverse roles in environmental persistence and/or animal virulence...
  13. doi request reprint Murine macrophage inflammatory cytokine production and immune activation in response to Vibrio parahaemolyticus infection
    Stephanie Waters
    Department of Biological Sciences, Wolf Hall, College of Arts and Sciences, University of Delaware, Newark, Delaware 19716, USA
    Microbiol Immunol 57:323-8. 2013
    ..The data presented here begin to identify the immune variables required to eliminate V. parahaemolyticus from infected host tissues...
  14. doi request reprint Bacteriophage-encoded bacterial virulence factors and phage-pathogenicity island interactions
    E Fidelma Boyd
    Department of Biological Sciences, University of Delaware, Newark, Delaware, USA
    Adv Virus Res 82:91-118. 2012
    ..A detailed examination of the filamentous phage CTXφ that encodes cholera toxin is given as the sole example to date of a single-stranded DNA phage that encodes a bacterial toxin...
  15. pmc Emergence of a virulent clade of Vibrio vulnificus and correlation with the presence of a 33-kilobase genomic island
    Ana Luisa V Cohen
    Department of Biological Sciences, University of Delaware, Newark, DE 19716, USA
    Appl Environ Microbiol 73:5553-65. 2007
    ..Our data suggest that lineage I may have a higher pathogenic potential and that region XII, along with other regions, may give isolates a selective advantage either in the human host or in the aquatic environment or both...
  16. pmc Three pathogenicity islands of Vibrio cholerae can excise from the chromosome and form circular intermediates
    Ronan A Murphy
    Department of Biological Sciences, University of Delaware, Newark, DE 19716, USA
    J Bacteriol 190:636-47. 2008
    ..These results show that all three pathogenicity islands can excise from the chromosome, which is likely a first step in their horizontal transfer...
  17. doi request reprint Loss of Sigma Factor RpoN Increases Intestinal Colonization of Vibrio parahaemolyticus in an Adult Mouse Model
    W Brian Whitaker
    Department of Biological Sciences, University of Delaware, Newark, Delaware, USA
    Infect Immun 82:544-56. 2014
    ..These data suggest that in V. parahaemolyticus, RpoN plays an important role in carbon utilization regulation, which may significantly affect host colonization. ..
  18. pmc Biosynthesis of the osmoprotectant ectoine, but not glycine betaine, is critical for survival of osmotically stressed Vibrio parahaemolyticus cells
    Serge Y Ongagna-Yhombi
    Department of Biological Sciences, University of Delaware, Newark, Delaware, USA
    Appl Environ Microbiol 79:5038-49. 2013
    ..The presence of both synthesis systems was the predominant distribution pattern among members of the Vibrionaceae family, suggesting this is the ancestral state. ..
  19. doi request reprint An atomic force microscopy method for the detection of binding forces between bacteria and a lipid bilayer containing higher order gangliosides
    Elizabeth L Adams
    Delaware Biotechnology Institute, University of Delaware, Newark, DE, USA
    J Microbiol Methods 84:352-4. 2011
    ..V. cholerae cells were covalently attached to AFM probes and forces were determined against a lipid bilayer containing sialic acid (N-acetylneuraminic acid) molecules as well as several control surfaces...
  20. pmc Sialic acid catabolism confers a competitive advantage to pathogenic vibrio cholerae in the mouse intestine
    Salvador Almagro-Moreno
    Department of Biological Sciences, University of Delaware, Newark, Delaware 19716, USA
    Infect Immun 77:3807-16. 2009
    ..Our data indicate an important relationship between the catabolism of sialic acid and bacterial pathogenesis, stressing the relevance of the utilization of the resources found in the host's environment...
  21. ncbi request reprint Mobilization of the Vibrio pathogenicity island between Vibrio cholerae isolates mediated by CP-T1 generalized transduction
    Yvonne A O'Shea
    Department of Microbiology, National University of Ireland, University College Cork, Cork, Ireland
    FEMS Microbiol Lett 214:153-7. 2002
    ..In this paper, we show that the VPI can be transferred between O1 serogroup strains, the predominant cause of epidemic cholera, via a generalized transducing phage CP-T1...
  22. ncbi request reprint The Vibrio seventh pandemic island-II is a 26.9 kb genomic island present in Vibrio cholerae El Tor and O139 serogroup isolates that shows homology to a 43.4 kb genomic island in V. vulnificus
    Yvonne A O'Shea
    Department of Microbiology, UCC, National University of Ireland, Cork, Ireland
    Microbiology 150:4053-63. 2004
    ..vulnificus strain, CMCP6, did not contain the 43.4 kb VVI-I; in this strain two ORFs were found between the 5' and 3' flanking genes VV10636 and VV10632, showing 100 % identity to VV0508 and VV0561, respectively, which flank VVI-I...
  23. ncbi request reprint Molecular evolution of Vibrio pathogenicity island-2 (VPI-2): mosaic structure among Vibrio cholerae and Vibrio mimicus natural isolates
    William S Jermyn
    Department of Microbiology, UCC, National University of Ireland Cork, Cork, Ireland
    Microbiology 151:311-22. 2005
    ..cholerae sequences; however, on the nanH gene tree, V. mimicus clustered with V. cholerae non-O1/non-O139 isolates, suggesting horizontal transfer of this region between these species...
  24. ncbi request reprint Genomic island identification in Vibrio vulnificus reveals significant genome plasticity in this human pathogen
    A M Quirke
    Department of Microbiology, University College Cork, National University of Ireland, Cork, Ireland
    Bioinformatics 22:905-10. 2006
    ..Supplementary information: Supplementary data are available at Bioinformatics online...
  25. pmc Four genomic islands that mark post-1995 pandemic Vibrio parahaemolyticus isolates
    Catherine C Hurley
    Department of Microbiology, University College Cork, National University of Ireland, Cork, Ireland
    BMC Genomics 7:104. 2006
    ..In 1996 the first appearance of a pandemic V. parahaemolyticus clone occurred, a new O3:K6 serotype strain that has now been identified worldwide as a major cause of seafood-borne gastroenteritis...
  26. pmc Genome diversity of Pseudomonas aeruginosa isolates from cystic fibrosis patients and the hospital environment
    Shirley Finnan
    Department of Microbiology, University College Cork, National University of Ireland, Cork, Ireland
    J Clin Microbiol 42:5783-92. 2004
    ..Our data demonstrate the significant role horizontal gene transfer and recombination, together with gene loss, play in the evolution of this important human pathogen...
  27. ncbi request reprint The genomic code: inferring Vibrionaceae niche specialization
    F Jerry Reen
    F Jerry Reen and Salvador Almagro Moreno are at the Department of Microbiology, University College Cork, National University of Ireland, Cork, Ireland
    Nat Rev Microbiol 4:697-704. 2006
    ..The existence of complete genome sequences for closely related species from varied aquatic niches makes this group an excellent case study for genome comparison...
  28. pmc Evolutionary genetic analysis of the emergence of epidemic Vibrio cholerae isolates on the basis of comparative nucleotide sequence analysis and multilocus virulence gene profiles
    Yvonne A O'Shea
    Department of Microbiology, University College Cork, National University of Ireland, Cork, Ireland
    J Clin Microbiol 42:4657-71. 2004
    ..cholerae natural isolates indicate that consecutive pandemic strains arose from a common O1 serogroup progenitor through the successive acquisition of new virulence regions...
  29. ncbi request reprint Investigation of a global collection of nontyphoidal Salmonella of various serotypes cultured between 1953 and 2004 for the presence of class 1 integrons
    Brenda P Murphy
    Veterinary Department, Cork County Council, County Hall, Cork, Ireland
    FEMS Microbiol Lett 266:170-6. 2007
    ..A Salmonella Wien isolate possessing a complete class 1 integron with a dfrA5-ereA2 gene arrangement within the variable region was characterized...
  30. doi request reprint Crystal structure of VC1805, a conserved hypothetical protein from a Vibrio cholerae pathogenicity island, reveals homology to human p32
    Md Arif Sheikh
    Centre for Biomolecular Sciences, University of St Andrews, St Andrews, Fife, KY16 9ST, United Kingdom
    Proteins 71:1563-71. 2008
  31. ncbi request reprint Evolutionary and functional analyses of variants of the toxin-coregulated pilus protein TcpA from toxigenic Vibrio cholerae non-O1/non-O139 serogroup isolates
    E Fidelma Boyd
    Department of Microbiology, National University of Ireland, University College Cork, Cork, Ireland
    Microbiology 148:1655-66. 2002
    ..All the V. cholerae non-O1 serogroup isolates tested were capable of colonizing the suckling mouse small intestine, suggesting that the different TcpA variants could function as colonization factors...