BRUCE BOWLER

Summary

Affiliation: University of Montana
Country: USA

Publications

  1. pmc Residual structure in unfolded proteins
    Bruce E Bowler
    Department of Chemistry and Biochemistry and Center for Biomolecular Structure and Dynamics, The University of Montana, Missoula, MT 59812, USA
    Curr Opin Struct Biol 22:4-13. 2012
  2. ncbi request reprint Thermodynamics of protein denatured states
    Bruce E Bowler
    Department of Chemistry, University of Montana, Missoula, MT 59812, USA
    Mol Biosyst 3:88-99. 2007
  3. ncbi request reprint Alkaline conformational transition and gated electron transfer with a Lys 79 --> his variant of iso-1-cytochrome c
    Swati Bandi
    Department of Chemistry and Center for Biomolecular Structure and Dynamics, The University of Montana, Missoula, Montana 59812, USA
    Biochemistry 46:10643-54. 2007
  4. ncbi request reprint Mutation of asparagine 52 to glycine promotes the alkaline form of iso-1-cytochrome c and causes loss of cooperativity in acid unfolding
    Saritha Baddam
    Department of Chemistry and Biochemistry, University of Denver, 2190 East Iliff Avenue, Denver, Colorado 80208 2436, USA
    Biochemistry 45:4611-9. 2006
  5. ncbi request reprint Effects of topology and excluded volume on protein denatured state conformational properties
    Christopher R Smith
    Department of Chemistry and Biochemistry, University of Denver, 2190 East Iliff Avenue, Denver, CO 80208, USA
    Biochemistry 41:10173-81. 2002
  6. ncbi request reprint Role of hydrogen bond networks and dynamics in positive and negative cooperative stabilization of a protein
    Jasmina S Redzic
    Department of Chemistry and Biochemistry, 2190 East Iliff Avenue, University of Denver, Denver, Colorado 80208 2436, USA
    Biochemistry 44:2900-8. 2005
  7. ncbi request reprint Communication of stabilizing energy between substructures of a protein
    Richard Kristinsson
    Department of Chemistry and Biochemistry, 2190 E Iliff Avenue, University of Denver, Denver, Colorado 80208 2436, USA
    Biochemistry 44:2349-59. 2005
  8. ncbi request reprint Conformational properties of the iso-1-cytochrome C denatured state: dependence on guanidine hydrochloride concentration
    Eydiejo Wandschneider
    Department of Chemistry and Biochemistry, 2190 E Iliff Avenue, University of Denver, Denver, CO 80208 2436, USA
    J Mol Biol 339:185-97. 2004
  9. ncbi request reprint Evaluation of cooperative interactions between substructures of iso-1-cytochrome c using double mutant cycles
    Eydiejo Wandschneider
    Department of Chemistry and Biochemistry, 2190 East Iliff Avenue, University of Denver, Denver, Colorado 80208 2436, USA
    Biochemistry 42:10659-66. 2003
  10. ncbi request reprint Proton-mediated dynamics of the alkaline conformational transition of yeast iso-1-cytochrome c
    Robert E Martinez
    Department of Chemistry and Biochemistry, University of Denver, 2190 East Iliff Avenue, Denver, Colorado 80208 2436, USA
    J Am Chem Soc 126:6751-8. 2004

Collaborators

  • Heinrich Roder
  • Saritha Baddam
  • Swati Bandi
  • Eydiejo Wandschneider
  • Christopher R Smith
  • Eydiejo Kurchan
  • Franco O Tzul
  • Richard Kristinsson
  • Jasmina S Redzic
  • Miao Miao Zhang
  • Robert E Martinez
  • Kenneth J Kise
  • Christine D Ford
  • Barbara N Hammack
  • Natasa Mateljevic

Detail Information

Publications19

  1. pmc Residual structure in unfolded proteins
    Bruce E Bowler
    Department of Chemistry and Biochemistry and Center for Biomolecular Structure and Dynamics, The University of Montana, Missoula, MT 59812, USA
    Curr Opin Struct Biol 22:4-13. 2012
    ....
  2. ncbi request reprint Thermodynamics of protein denatured states
    Bruce E Bowler
    Department of Chemistry, University of Montana, Missoula, MT 59812, USA
    Mol Biosyst 3:88-99. 2007
    ..New insights into protein folding and novel methods to manipulate protein stability are emerging from this work...
  3. ncbi request reprint Alkaline conformational transition and gated electron transfer with a Lys 79 --> his variant of iso-1-cytochrome c
    Swati Bandi
    Department of Chemistry and Center for Biomolecular Structure and Dynamics, The University of Montana, Missoula, Montana 59812, USA
    Biochemistry 46:10643-54. 2007
    ..Both the position and the nature of the alkaline state ligand modulate the dynamics of ET gating...
  4. ncbi request reprint Mutation of asparagine 52 to glycine promotes the alkaline form of iso-1-cytochrome c and causes loss of cooperativity in acid unfolding
    Saritha Baddam
    Department of Chemistry and Biochemistry, University of Denver, 2190 East Iliff Avenue, Denver, Colorado 80208 2436, USA
    Biochemistry 45:4611-9. 2006
    ..Both the Lys 79 --> Ala and Asn 52 --> Gly mutations are expected to affect the buried hydrogen bond network of cytochrome c, suggesting that this network is an important modulator of the acid unfolding of cytochrome c...
  5. ncbi request reprint Effects of topology and excluded volume on protein denatured state conformational properties
    Christopher R Smith
    Department of Chemistry and Biochemistry, University of Denver, 2190 East Iliff Avenue, Denver, CO 80208, USA
    Biochemistry 41:10173-81. 2002
    ..These data provide direct evidence that denatured state topology affects contact probability, and thus probable folding pathways, in a disordered protein...
  6. ncbi request reprint Role of hydrogen bond networks and dynamics in positive and negative cooperative stabilization of a protein
    Jasmina S Redzic
    Department of Chemistry and Biochemistry, 2190 East Iliff Avenue, University of Denver, Denver, Colorado 80208 2436, USA
    Biochemistry 44:2900-8. 2005
    ..9 +/-0.7 kcal/mol) that combined with cancellation of cooperative and anticooperative pairwise interactions produce apparent additivity for the stabilizing effects of the single mutations in the triple mutant variant...
  7. ncbi request reprint Communication of stabilizing energy between substructures of a protein
    Richard Kristinsson
    Department of Chemistry and Biochemistry, 2190 E Iliff Avenue, University of Denver, Denver, Colorado 80208 2436, USA
    Biochemistry 44:2349-59. 2005
    ....
  8. ncbi request reprint Conformational properties of the iso-1-cytochrome C denatured state: dependence on guanidine hydrochloride concentration
    Eydiejo Wandschneider
    Department of Chemistry and Biochemistry, 2190 E Iliff Avenue, University of Denver, Denver, CO 80208 2436, USA
    J Mol Biol 339:185-97. 2004
    ..The data from these studies provide direct insight into the relative probability with which loops of different sizes will form, as well as the factors which affect loop formation...
  9. ncbi request reprint Evaluation of cooperative interactions between substructures of iso-1-cytochrome c using double mutant cycles
    Eydiejo Wandschneider
    Department of Chemistry and Biochemistry, 2190 East Iliff Avenue, University of Denver, Denver, Colorado 80208 2436, USA
    Biochemistry 42:10659-66. 2003
    ..The data are consistent with an entropic stabilization of the intersubstructure hydrogen bond between His 26 and Glu 44 by the Ile 52 mutation...
  10. ncbi request reprint Proton-mediated dynamics of the alkaline conformational transition of yeast iso-1-cytochrome c
    Robert E Martinez
    Department of Chemistry and Biochemistry, University of Denver, 2190 East Iliff Avenue, Denver, Colorado 80208 2436, USA
    J Am Chem Soc 126:6751-8. 2004
    ..6 and has unmasked two other ionizable groups, besides the ligand replacing Met 80, that modulate the kinetics of this process. The results are discussed in terms of the impact of ionization equilibria on protein folding mechanisms...
  11. doi request reprint Probing the bottom of a folding funnel using conformationally gated electron transfer reactions
    Swati Bandi
    Department of Chemistry and Center for Biomolecular Structure and Dynamics, The University of Montana, Missoula, Montana 59812, USA
    J Am Chem Soc 130:7540-1. 2008
    ..Thus, funneled landscapes have evolved such that loss of global stability lowers barriers at the bottom of a folding funnel, still allowing for efficient folding...
  12. ncbi request reprint Effect of pH on the iso-1-cytochrome c denatured state: changing constraints due to heme ligation
    Christopher R Smith
    Department of Chemistry and Biochemistry, University of Denver, 2190 East Iliff Avenue, Denver, Colorado 80208, USA
    Biochemistry 42:2174-84. 2003
    ..Surprisingly, the heme-Trp 59 distance in the His 54 bound conformation only drops to approximately 21 A, consistent with an extended conformation for the short polypeptide segment separating heme and Trp 59...
  13. pmc Sequence composition effects on denatured state loop formation in iso-1-cytochrome c variants: polyalanine versus polyglycine inserts
    Franco O Tzul
    Department of Chemistry and Center for Biomolecular Structure and Dynamics, University of Montana, Missoula, MT 59812, USA
    J Mol Biol 371:577-84. 2007
    ..The underlying basis for the similar behavior of loops with polyalanine versus polyglycine inserts is discussed in terms of the current knowledge of the structure and loop formation kinetics of glycine versus alanine-rich peptides...
  14. ncbi request reprint Electron transfer through a prenucleated bimetalated alanine-based peptide helix
    Kenneth J Kise
    Department of Chemistry and Biochemistry, University of Denver, 2190 East Iliff Avenue, Denver, Colorado 80208 2436, USA
    Inorg Chem 42:3891-7. 2003
    ..19 +/- 0.4 cm(-)(1). Analysis in terms of the pathway model for electronic coupling indicates that this magnitude of H(ab) is consistent with the participation of hydrogen bonds in electronic coupling for an isolated alpha-helix...
  15. ncbi request reprint Tuning the rate and pH accessibility of a conformational electron transfer gate
    Saritha Baddam
    Department of Chemistry and Biochemistry, University of Denver, 2190 East Iliff Avenue, Denver, CO 80208 2436, USA
    Inorg Chem 45:6338-46. 2006
    ..Soc. 2005, 127, 9702-9703), the A79H73 variant allows His 73-heme-mediated ET gating to be fine-tuned from 75 to 200 ms. The slower Lys 73-heme (15-20 s time scale) ET gate for the A79G52 variant is now accessible over the pH range 6-8...
  16. ncbi request reprint Thermodynamics and kinetics of formation of the alkaline state of a Lys 79-->Ala/Lys 73-->His variant of iso-1-cytochrome c
    Saritha Baddam
    Department of Chemistry and Biochemistry, University of Denver, 2190 East Iliff Avenue, Denver, Colorado 80208 2436, USA
    Biochemistry 44:14956-68. 2005
    ..The proline isomerization phase is modulated by both pK(HL) and pK(H2) indicating that it is sensitive to protein conformation...
  17. ncbi request reprint Kinetics of loop formation and breakage in the denatured state of iso-1-cytochrome c
    Eydiejo Kurchan
    Department of Chemistry and Biochemistry, 2190 E Iliff Avenue, University of Denver, Denver, CO 80208 2436, USA
    J Mol Biol 353:730-43. 2005
    ..The implications of the kinetics of loop formation and breakage in the denatured state for the mechanism of protein folding are discussed...
  18. ncbi request reprint Estimation of the compaction of the denatured state by a protein variant involved in a reverse hydrophobic effect
    Miao Miao Zhang
    Department of Chemistry and Biochemistry, University of Denver, Denver, CO 80208 2436, USA
    Protein J 23:119-26. 2004
    ..Given that position 73 is beyond Trp 59 in the sequence, the denatured state compaction appears to be a global effect...
  19. ncbi request reprint Conformationally gated electron transfer in iso-1-cytochrome c: engineering the rate of a conformational switch
    Saritha Baddam
    Department of Chemistry and Biochemistry, University of Denver, Colorado 80208, USA
    J Am Chem Soc 127:9702-3. 2005
    ....

Research Grants6

  1. MEASUREMENT OF CHANGES IN DENATURED STATE ENERGETICS
    BRUCE BOWLER; Fiscal Year: 2003
    ..In sum, the proposed experiments use a novel thermodynamic approach to probe key parameters of protein denatured states expected to be principal modulators of early events in protein folding. ..
  2. Conformational Properties of Protein Denatured States
    BRUCE BOWLER; Fiscal Year: 2005
    ..Our multipronged approach probes key parameters of protein denatured states, expected to be principal modulators of early events in protein folding. ..
  3. Conformational Properties of Protein Denatured States
    BRUCE BOWLER; Fiscal Year: 2007
    ..Our multipronged approach probes key parameters of protein denatured states expectedto be principal modulators of early events in protein folding. ..
  4. Conformational Properties of Protein Denatured States
    BRUCE BOWLER; Fiscal Year: 2009
    ..Our multipronged approach probes key parameters of protein denatured states expectedto be principal modulators of early events in protein folding. ..