P Bornstein

Summary

Affiliation: University of Washington
Country: USA

Publications

  1. ncbi Thrombospondin-2 is essential for myocardial matrix integrity: increased expression identifies failure-prone cardiac hypertrophy
    Blanche Schroen
    Experimental and Molecular Cardiology CARIM, University of Maastricht, Maastricht, The Netherlands
    Circ Res 95:515-22. 2004
  2. pmc Proteolysis of cell-surface tissue transglutaminase by matrix metalloproteinase-2 contributes to the adhesive defect and matrix abnormalities in thrombospondin-2-null fibroblasts and mice
    Azin Agah
    Department of Biochemistry, School of Medicine, University of Washington, Seattle, Washington, USA
    Am J Pathol 167:81-8. 2005
  3. ncbi Low-density lipoprotein receptor-related protein contributes to the antiangiogenic activity of thrombospondin-2 in a murine glioma model
    Constance Y Fears
    Departments of Pathology and Cell Biology, University of Alabama at Birmingham, Birmingham, AL 35294 0007, USA
    Cancer Res 65:9338-46. 2005
  4. pmc Disruption of the thrombospondin-2 gene alters the lamellar morphology but does not permit vascularization of the adult mouse lumbar disc
    Helen E Gruber
    Department of Orthopaedic Surgery, Carolinas Medical Center, Charlotte, NC 28232, USA
    Arthritis Res Ther 10:R96. 2008
  5. ncbi Mice with a deletion in the first intron of the Col1a1 gene develop age-dependent aortic dissection and rupture
    Otto Rahkonen
    Department of Medical Biochemistry and Molecular Biology, University of Turku, Finland
    Circ Res 94:83-90. 2004
  6. ncbi Thrombospondin 2, a matricellular protein with diverse functions
    P Bornstein
    Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
    Matrix Biol 19:557-68. 2000
  7. ncbi Matricellular proteins: extracellular modulators of cell function
    Paul Bornstein
    Department of Biochemistry, Box 357350, University of Washington, Seattle, WA 98195, USA
    Curr Opin Cell Biol 14:608-16. 2002
  8. ncbi Thrombospondin 2 modulates collagen fibrillogenesis and angiogenesis
    P Bornstein
    Department of Biochemistry, University of Washington, Seattle 98195, USA
    J Investig Dermatol Symp Proc 5:61-6. 2000
  9. ncbi The role of thrombospondins 1 and 2 in the regulation of cell-matrix interactions, collagen fibril formation, and the response to injury
    Paul Bornstein
    Department of Biochemistry, Box 357350, University of Washington, Seattle, WA 98195, USA
    Int J Biochem Cell Biol 36:1115-25. 2004
  10. ncbi The globular domain of the proalpha 1(I) N-propeptide is not required for secretion, processing by procollagen N-proteinase, or fibrillogenesis of type I collagen in mice
    Paul Bornstein
    Department of Biochemistry, The University of Washington, Seattle, Washington 98195, USA
    J Biol Chem 277:2605-13. 2002

Detail Information

Publications80

  1. ncbi Thrombospondin-2 is essential for myocardial matrix integrity: increased expression identifies failure-prone cardiac hypertrophy
    Blanche Schroen
    Experimental and Molecular Cardiology CARIM, University of Maastricht, Maastricht, The Netherlands
    Circ Res 95:515-22. 2004
    ..This suggests that expression of TSP2 marks an early-stage molecular program that is activated uniquely in hypertrophied hearts that are prone to fail...
  2. pmc Proteolysis of cell-surface tissue transglutaminase by matrix metalloproteinase-2 contributes to the adhesive defect and matrix abnormalities in thrombospondin-2-null fibroblasts and mice
    Azin Agah
    Department of Biochemistry, School of Medicine, University of Washington, Seattle, Washington, USA
    Am J Pathol 167:81-8. 2005
    ..These findings provide a mechanism for the reduced adhesion of TSP-2-null fibroblasts and an explanation for the increased collagen solubility and fragility of TSP-2-null skin...
  3. ncbi Low-density lipoprotein receptor-related protein contributes to the antiangiogenic activity of thrombospondin-2 in a murine glioma model
    Constance Y Fears
    Departments of Pathology and Cell Biology, University of Alabama at Birmingham, Birmingham, AL 35294 0007, USA
    Cancer Res 65:9338-46. 2005
    ..Thus, the antiangiogenic activity of TSP-2 is capable of inhibiting the growth of gliomas in part by reducing the levels of MMP-2 in the tumor microvasculature. This mechanism is mediated by LRP1...
  4. pmc Disruption of the thrombospondin-2 gene alters the lamellar morphology but does not permit vascularization of the adult mouse lumbar disc
    Helen E Gruber
    Department of Orthopaedic Surgery, Carolinas Medical Center, Charlotte, NC 28232, USA
    Arthritis Res Ther 10:R96. 2008
    ..In the present study we have examined thrombospondin-2 (TSP-2), a matricellular protein with recognized anti-angiogenic activity in vivo and in vitro...
  5. ncbi Mice with a deletion in the first intron of the Col1a1 gene develop age-dependent aortic dissection and rupture
    Otto Rahkonen
    Department of Medical Biochemistry and Molecular Biology, University of Turku, Finland
    Circ Res 94:83-90. 2004
    ....
  6. ncbi Thrombospondin 2, a matricellular protein with diverse functions
    P Bornstein
    Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
    Matrix Biol 19:557-68. 2000
    ..The investigation of the basis for the other defects in the TSP2-null mouse is likely to yield equally interesting results...
  7. ncbi Matricellular proteins: extracellular modulators of cell function
    Paul Bornstein
    Department of Biochemistry, Box 357350, University of Washington, Seattle, WA 98195, USA
    Curr Opin Cell Biol 14:608-16. 2002
    ..In many cases, aspects of these phenotypes have illuminated previously unsuspected consequences of the lack of appropriate interactions of cells with their environment...
  8. ncbi Thrombospondin 2 modulates collagen fibrillogenesis and angiogenesis
    P Bornstein
    Department of Biochemistry, University of Washington, Seattle 98195, USA
    J Investig Dermatol Symp Proc 5:61-6. 2000
    ..Experiments are proposed to distinguish between a primarily endothelial cell versus an extracellular matrix origin for the increased angiogenesis in TSP2-null mice...
  9. ncbi The role of thrombospondins 1 and 2 in the regulation of cell-matrix interactions, collagen fibril formation, and the response to injury
    Paul Bornstein
    Department of Biochemistry, Box 357350, University of Washington, Seattle, WA 98195, USA
    Int J Biochem Cell Biol 36:1115-25. 2004
    ....
  10. ncbi The globular domain of the proalpha 1(I) N-propeptide is not required for secretion, processing by procollagen N-proteinase, or fibrillogenesis of type I collagen in mice
    Paul Bornstein
    Department of Biochemistry, The University of Washington, Seattle, Washington 98195, USA
    J Biol Chem 277:2605-13. 2002
    ..These findings suggest that although the N-propeptide is not essential for collagen biogenesis in mice it may play some essential role during embryonic development...
  11. pmc SP1-binding elements, within the common metaxin-thrombospondin 3 intergenic region, participate in the regulation of the metaxin gene
    M Collins
    Department of Biochemistry, University of Washington, Seattle 98195, USA
    Nucleic Acids Res 24:3661-9. 1996
    ..Thus, despite the potential for common regulatory mechanisms, the available evidence indicates that the Mtx minimal promoter does not significantly affect Thbs3 gene expression...
  12. ncbi Analysis of the promoter and transcription start sites of the human thrombospondin 2 gene (THBS2)
    K W Adolph
    Department of Biochemistry, University of Minnesota Medical School, Minneapolis 55455, USA
    Gene 193:5-11. 1997
    ..These results suggest that tissue-specific differences in expression of the THBS2 gene may be determined, in part, by selection of the transcription start site and resulting differences in the 5' untranslated region...
  13. ncbi A far upstream, cell type-specific enhancer of the mouse thrombospondin 3 gene is located within intron 6 of the adjacent metaxin gene
    M Collins
    Department of Biochemistry, University of Washington, Seattle, Washington 98195, USA
    J Biol Chem 273:21816-24. 1998
    ..coli lacZ gene in transgenic mice, whereas a 2-kilobase pair promoter that lacked the enhancer was inactive. Thus, despite their close proximity, the genes of the Mtx/Thbs3 gene cluster are regulated independently...
  14. ncbi Isolation and characterization of the mouse thrombospondin 3 (Thbs3) gene
    P Bornstein
    Department of Biochemistry, University of Washington, Seattle 98195
    Genomics 15:607-13. 1993
    ..Based on its structure, we suggest that TSP3 may play both a unique role in cell-matrix interactions and perform functions that overlap with those of TSP1 and TSP2...
  15. ncbi The distribution of the matricellular protein thrombospondin 2 in tissues of embryonic and adult mice
    T R Kyriakides
    Department of Biochemistry, University of Washington, Seattle, Washington, USA
    J Histochem Cytochem 46:1007-15. 1998
    ..In adult tissues, TSP2 is located in the pericellular environment, where it can potentially influence the cell-matrix interactions associated with cell movement and tissue repair...
  16. ncbi Characterization of the mouse thrombospondin gene and evaluation of the role of the first intron in human gene expression
    P Bornstein
    Department of Biochemistry, University of Washington, Seattle 98195
    J Biol Chem 265:16691-8. 1990
    ....
  17. ncbi The human thrombospondin 3 gene: analysis of transcription initiation and an alternatively spliced transcript
    K W Adolph
    Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis 55455, USA
    Mol Cell Biol Res Commun 2:47-52. 1999
    ..The complexities of TSP3 transcription initiation revealed by this study could contribute to the tissue-specific expression and diverse functions of TSP3...
  18. ncbi Differential expression of thrombospondin 1, 2, and 3 during murine development
    M L Iruela-Arispe
    Department of Biological Structure, University of Washington, Seattle 98195
    Dev Dyn 197:40-56. 1993
    ..Although thrombospondin 1, 2, and 3 belong to a family of structurally related genes, the differences observed in the spatiotemporal distribution of the corresponding mRNAs indicate unique functions for these secreted proteins...
  19. pmc Mice that lack thrombospondin 2 display connective tissue abnormalities that are associated with disordered collagen fibrillogenesis, an increased vascular density, and a bleeding diathesis
    T R Kyriakides
    Department of Biochemistry, University of Washington, Seattle, Washington 98195, USA
    J Cell Biol 140:419-30. 1998
    ....
  20. ncbi Regulation of angiogenesis and matrix remodeling by localized, matrix-mediated antisense gene delivery
    T R Kyriakides
    Department of Biochemistry, University of Washington, Seattle, Washington 98195, USA
    Mol Ther 3:842-9. 2001
    ..This method of antisense DNA delivery has the potential to improve the performance and life span of implantable delivery devices and biosensors...
  21. ncbi Structure and organization of the human metaxin gene (MTX) and pseudogene
    G L Long
    Department of Biochemistry, University of Washington, Seattle 98195, USA
    Genomics 33:177-84. 1996
    ..Thus, there is a 278 amino acid open reading frame that is 97.8% identical to metaxin. However, psMTX lacks the first intron and promoter present in MTX, and at least in liver, the pseudogene is not expressed...
  22. ncbi Characterization of the mouse thrombospondin 2 gene
    T Shingu
    Department of Biochemistry, University of Washington, Seattle 98195
    Genomics 16:78-84. 1993
    ..The structure of the TSP2 gene is consistent with emerging evidence supporting the view that TSP1 and TSP2 perform overlapping but distinct functions...
  23. ncbi Overlapping Egr-1 and Sp1 sites function in the regulation of transcription of the mouse thrombospondin 1 gene
    T Shingu
    Department of Biochemistry, University of Washington, Seattle 98195
    J Biol Chem 269:32551-7. 1994
    ..These experiments add support to the growing realization that similar physiologic responses of homologous genes in mouse and man need not utilize similarly placed cis-acting elements...
  24. pmc Altered extracellular matrix remodeling and angiogenesis in sponge granulomas of thrombospondin 2-null mice
    T R Kyriakides
    Department of Biochemistry, University of Washington, Seattle, Washington 98195, USA
    Am J Pathol 159:1255-62. 2001
    ..In addition, they provide in vivo evidence for a newly proposed function of TSP2 as a modulator of extracellular MMP2 levels...
  25. pmc Mice that lack the angiogenesis inhibitor, thrombospondin 2, mount an altered foreign body reaction characterized by increased vascularity
    T R Kyriakides
    Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 96:4449-54. 1999
    ..The expression of TSP2 therefore could represent a molecular target for local inhibitory measures when vascularization of the tissue surrounding an implanted device is desired...
  26. ncbi SPARC-thrombospondin-2-double-null mice exhibit enhanced cutaneous wound healing and increased fibrovascular invasion of subcutaneous polyvinyl alcohol sponges
    Pauli A Puolakkainen
    Hope Heart Program, Benaroya Research Institute at Virginia Mason, 1201 9th Ave, Seattle, WA 98101, USA
    J Histochem Cytochem 53:571-81. 2005
    ..SPARC and TSP-2 therefore perform similar functions in the regulation of cutaneous wound healing, but fine-tuning with respect to ECM production and remodeling could account for the enhanced response seen in ST-double-null mice...
  27. pmc A gene-targeting approach identifies a function for the first intron in expression of the alpha1(I) collagen gene
    S G Hormuzdi
    Department of Biochemistry, University of Washington, Seattle, Washington 98195, USA
    Mol Cell Biol 18:3368-75. 1998
    ..Our experiments demonstrate the utility of gene-targeting techniques that produce subtle mutations for studies of cis-acting elements in gene regulation...
  28. ncbi Increased marrow-derived osteoprogenitor cells and endosteal bone formation in mice lacking thrombospondin 2
    K D Hankenson
    Department of Biochemistry, University of Washington, Seattle 98195 7350, USA
    J Bone Miner Res 15:851-62. 2000
    ..We conclude that TSP2 regulates the proliferation of osteoblast progenitors, directly or indirectly, and that in its absence endosteal bone formation is increased...
  29. pmc Regulatory elements in the first intron contribute to transcriptional control of the human alpha 1(I) collagen gene
    P Bornstein
    Department of Biochemistry, University of Washington, Seattle
    Proc Natl Acad Sci U S A 84:8869-73. 1987
    ..We propose that normal regulation of alpha 1(I) collagen gene transcription results from an interplay of positive and negative elements present in the promoter region and within the first intron...
  30. pmc Metaxin, a gene contiguous to both thrombospondin 3 and glucocerebrosidase, is required for embryonic development in the mouse: implications for Gaucher disease
    P Bornstein
    Department of Biochemistry, University of Washington, Seattle 98195, USA
    Proc Natl Acad Sci U S A 92:4547-51. 1995
    ..Clearly, the contiguous gene organization at this locus limits targeting strategies for the production of murine models of Gaucher disease...
  31. ncbi Mice with a targeted intronic deletion in the Col1a1 gene respond to bleomycin-induced pulmonary fibrosis with increased expression of the mutant allele
    S G Hormuzdi
    Department of Biochemistry, University of Washington, Seattle 98195, USA
    Matrix Biol 18:287-94. 1999
    ..This model has the potential to resolve the conflicting results of previous transfection and transgenic experiments in which different fragments of the Col1a1 gene were used...
  32. ncbi Metaxin 1 interacts with metaxin 2, a novel related protein associated with the mammalian mitochondrial outer membrane
    L C Armstrong
    Department of Biochemistry, University of Washington, Seattle 98195 7350, USA
    J Cell Biochem 74:11-22. 1999
    ....
  33. pmc A second thrombospondin gene in the mouse is similar in organization to thrombospondin 1 but does not respond to serum
    P Bornstein
    Department of Biochemistry, University of Washington, Seattle 98195
    Proc Natl Acad Sci U S A 88:8636-40. 1991
    ..In marked contrast to Thbs1, the Thbs2 gene is not induced by serum in NIH 3T3 cells; promoter sequences in the two genes are also very different. It is therefore likely that the two TSPs perform related but distinct functions...
  34. ncbi Regulation of expression of the alpha 1 (I) collagen gene: a critical appraisal of the role of the first intron
    P Bornstein
    Department of Biochemistry University of Washington, Seattle, Washington, USA
    Matrix Biol 15:3-10. 1996
    ....
  35. ncbi Thrombospondin 2 levels are increased in aged mice: consequences for cutaneous wound healing and angiogenesis
    Azin Agah
    Departments of Biochemistry, University of Washington, Box 357350, Seattle, WA 98195, USA
    Matrix Biol 22:539-47. 2004
    ..We suggest that the delay in expression of TSP2 and MMP2 in the wounds of aged mice could contribute to their impaired rate of wound healing...
  36. ncbi Transcriptional repression of the alpha 1(I) collagen gene by ras is mediated in part by an intronic AP1 site
    J L Slack
    Department of Biochemistry, University of Washington, Seattle 98195, USA
    J Cell Biochem 58:380-92. 1995
    ..We conclude that ras transformation suppresses the function of a cell-specific enhancer in the first intron of the alpha 1(I) collagen gene...
  37. ncbi Modulation of thrombospondin expression during differentiation of embryonal carcinoma cells
    D J Liska
    Department of Biochemistry, University of Washington, Seattle 98195
    J Cell Physiol 158:495-505. 1994
    ..The response of TSP1 and TSP2 to differentiation of P19EC cells indicates that this cell system will serve as a valuable model for the study of TSP expression and function during neurogenesis...
  38. ncbi Extracellular matrix metalloproteinase 2 levels are regulated by the low density lipoprotein-related scavenger receptor and thrombospondin 2
    Z Yang
    Department of Biochemistry, The University of Washington, Seattle, Washington 98195, USA
    J Biol Chem 276:8403-8. 2001
    ..Thus, some features of the phenotype of TSP2-null mice, such as abnormal collagen fibrillogenesis, accelerated wound healing, and increased angiogenesis, could result in part from increased MMP2 activity...
  39. ncbi The secreted protein thrombospondin 2 is an autocrine inhibitor of marrow stromal cell proliferation
    Kurt D Hankenson
    Department of Biochemistry, University of Washington, Seattle 98195 7350, USA
    J Bone Miner Res 17:415-25. 2002
    ..We conclude that the increased endosteal bone formation observed in TSP2-null mice results primarily from the failure of TSP2 to regulate locally MSC cell cycle progression...
  40. pmc The lack of thrombospondin-1 (TSP1) dictates the course of wound healing in double-TSP1/TSP2-null mice
    Azin Agah
    Department of Biochemistry, University of Washington, Seattle 98195, USA
    Am J Pathol 161:831-9. 2002
    ..These findings reflect distinct temporal and spatial expressions of TSP1 and TSP2 in the healing wound...
  41. ncbi Mapping of the thrombospondin gene to human chromosome 15 and mouse chromosome 2 by in situ hybridization
    E Jaffe
    Department of Pathology, University of Washington, Seattle 98195
    Genomics 7:123-6. 1990
    ..These results also confirm a region of homology between the proximal part of human chromosome 15 and region F of mouse chromosome 2...
  42. ncbi Accelerated wound healing in mice with a disruption of the thrombospondin 2 gene
    T R Kyriakides
    Department of Biochemistry, University of Washington, Seattle 98195, USA
    J Invest Dermatol 113:782-7. 1999
    ....
  43. ncbi Metaxin is a component of a preprotein import complex in the outer membrane of the mammalian mitochondrion
    L C Armstrong
    Department of Biochemistry, University of Washington, Seattle, Washington 98195, USA
    J Biol Chem 272:6510-8. 1997
    ....
  44. ncbi Structural and functional analysis of the first intron of the human alpha 2(I) collagen-encoding gene
    A L Sherwood
    Department of Biochemistry, University of Washington, Seattle 98195
    Gene 89:239-44. 1990
    ....
  45. ncbi Structure and organization of the human thrombospondin 3 gene (THBS3)
    K W Adolph
    Department of Biochemistry, University of Washington, Seattle 98195, USA
    Genomics 27:329-36. 1995
    ....
  46. pmc Thrombospondin 2 inhibits microvascular endothelial cell proliferation by a caspase-independent mechanism
    Lucas C Armstrong
    Department of Biochemistry, University of Washington, Seattle, Washington 98195, USA
    Mol Biol Cell 13:1893-905. 2002
    ....
  47. ncbi Differential expression of SPARC and thrombospondin 1 in wound repair: immunolocalization and in situ hybridization
    M J Reed
    Department of Medicine, University of Washington, Seattle
    J Histochem Cytochem 41:1467-77. 1993
    ....
  48. ncbi Megakaryocytes require thrombospondin-2 for normal platelet formation and function
    Themis R Kyriakides
    Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
    Blood 101:3915-23. 2003
    ..We conclude that the uptake of TSP2 by MKs from the marrow milieu is required for proper MK function and the release of functionally competent platelets...
  49. ncbi The NH(2)-terminal propeptides of fibrillar collagens: highly conserved domains with poorly understood functions
    Paul Bornstein
    Department of Biochemistry, University of Washington, WA, Seattle 98195, USA
    Matrix Biol 21:217-26. 2002
    ..Experiments to examine these possibilities are proposed...
  50. ncbi Covalent cross-links in collagen: a personal account of their discovery
    Paul Bornstein
    Department of Biochemistry, University of Washington, P O Box 357350, Seattle, WA 98195, USA
    Matrix Biol 22:385-91. 2003
  51. pmc Thrombospondins use the VLDL receptor and a nonapoptotic pathway to inhibit cell division in microvascular endothelial cells
    Anush Oganesian
    Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
    Mol Biol Cell 19:563-71. 2008
    ....
  52. ncbi Matricellular proteins as modulators of wound healing and the foreign body response
    Themis R Kyriakides
    Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
    Thromb Haemost 90:986-92. 2003
    ..This review will focus on the roles of these proteins in the response to injury in mice and will show how studies of this pathophysiological process can elucidate some of the intrinsic properties of these matricellular proteins...
  53. ncbi A second, expressed thrombospondin gene (Thbs2) exists in the mouse genome
    P Bornstein
    Department of Biochemistry, University of Washington, Seattle 98195
    J Biol Chem 266:12821-4. 1991
    ....
  54. pmc The CC chemokine ligand, CCL2/MCP1, participates in macrophage fusion and foreign body giant cell formation
    Themis R Kyriakides
    Department of Biochemistry, University of Washington, Seattle, Washington, USA
    Am J Pathol 165:2157-66. 2004
    ..Our findings demonstrate a previously unreported involvement of CCL2 in FBGC formation, and suggest that FBGC are not the primary determinants of capsule formation in the FBR...
  55. pmc The NH2-terminal propeptide of type I procollagen acts intracellularly to modulate cell function
    Anush Oganesian
    Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
    J Biol Chem 281:38507-18. 2006
    ..Our findings suggest that N-propeptide performs an important feedback regulatory function and provides a rationale for the prominence of a homotrimeric form of type I procollagen (alpha1 trimer) during vertebrate development...
  56. ncbi pH-sensitive polymers that enhance intracellular drug delivery in vivo
    Themis R Kyriakides
    Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
    J Control Release 78:295-303. 2002
    ..These results suggest that PPAA can provide significant improvements in the in vivo efficacy of drugs such as DNA...
  57. ncbi Thrombospondins 1 and 2 function as inhibitors of angiogenesis
    Lucas C Armstrong
    Department of Biochemistry, University of Washington, P O Box 357350, Seattle 98195 7350, USA
    Matrix Biol 22:63-71. 2003
    ..This finding raises the possibility that TSPs can activate separate cell death and anti-proliferative pathways...
  58. ncbi Cell-matrix interactions: the view from the outside
    Paul Bornstein
    Departments of Biochemistry and Medicine, University of Washington, Seattle, Washington 98195, USA
    Methods Cell Biol 69:7-11. 2002
  59. ncbi Highly conserved sequences in the 3'-untranslated region of the COL1A1 gene bind cell-specific nuclear proteins
    A Maatta
    Department of Medical Biochemistry, University of Turku, Finland
    FEBS Lett 279:9-13. 1991
    ..The second conserved domain includes a motif shared with several collagen genes. Both conserved domains bind cell-specific nuclear proteins suggesting that the 3'-UTR is important for cell specific expression of the COL1A1 gene...
  60. pmc Compromised production of extracellular matrix in mice lacking secreted protein, acidic and rich in cysteine (SPARC) leads to a reduced foreign body reaction to implanted biomaterials
    Pauli Puolakkainen
    Department of Vascular Biology, The Hope Heart Institute, University of Washington, Seattle, Washington 98104, USA
    Am J Pathol 162:627-35. 2003
    ..The altered ECM resulting in decreased capsular thickness, indicative of an altered foreign body reaction in SPARC-null mice, implicates SPARC as an important modulator of the encapsulation of implanted biomaterials...
  61. ncbi Thrombospondin 2 functions as an endogenous regulator of angiogenesis and inflammation in experimental glomerulonephritis in mice
    Christoph Daniel
    Department of Nephrology and Hypertension, Universitat Erlangen Nurnberg, Loschgestrasse 8, 91054 Erlangen, Germany
    J Am Soc Nephrol 18:788-98. 2007
    ....
  62. ncbi Interactions between CD47 and thrombospondin reduce inflammation
    Laurence Lamy
    INSERM Unit 576, Hospitalier de l Université de Nice, University of Nice Sophia Antipolis, hôpital de l Archet I, 151 rue Saint Antoine de Ginestière, Nice, France
    J Immunol 178:5930-9. 2007
    ..Overall, these results reveal an important role in vivo for this new CD47/BNIP3 pathway in limiting inflammation by controlling the number of activated T cells...
  63. ncbi SPARC regulates processing of procollagen I and collagen fibrillogenesis in dermal fibroblasts
    Tyler J Rentz
    Department of Medicine, Medical University of South Carolina, Charleston, SC 29412, USA
    J Biol Chem 282:22062-71. 2007
    ..We conclude that SPARC mediates the association of procollagen I with cells, as well as its processing and incorporation into the extracellular matrix...
  64. ncbi Increased and prolonged inflammation and angiogenesis in delayed-type hypersensitivity reactions elicited in the skin of thrombospondin-2--deficient mice
    Bernhard Lange-Asschenfeldt
    Cutaneous Biology Research Center, Department of Dermatology, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA 02129, USA
    Blood 99:538-45. 2002
    ..These results reveal an important role of TSP-2 in limiting the extent and the duration of edema formation, angiogenesis, and inflammatory cell infiltration during acute and chronic inflammation...
  65. pmc Skeletal abnormalities in mice lacking extracellular matrix proteins, thrombospondin-1, thrombospondin-3, thrombospondin-5, and type IX collagen
    Karen L Posey
    Department of Pediatrics, University of Texas Medical School, 6431 Fannin, Houston, TX 77030, USA
    Am J Pathol 172:1664-74. 2008
    ....
  66. pmc Thrombospondin-2 modulates extracellular matrix remodeling during physiological angiogenesis
    Marie M Krady
    Department of Pathology, Yale University School of Medicine, New Haven, CT 06536 9812, USA
    Am J Pathol 173:879-91. 2008
    ..Taken together, our observations implicate the matrix-modulating activity of TSP2 as a mechanism by which physiological angiogenesis is inhibited...
  67. pmc Thrombospondins deployed by thrombopoietic cells determine angiogenic switch and extent of revascularization
    Hans Georg Kopp
    Howard Hughes Medical Institute, Department of Genetic Medicine, Weill Medical College of Cornell University, New York, NY 10021, USA
    J Clin Invest 116:3277-91. 2006
    ..As such, interference with the release of cellular stores of TSPs may be clinically effective in augmenting neoangiogenesis...
  68. ncbi Roles of thrombospondin-1 and -2 in regulating corneal and iris angiogenesis
    Claus Cursiefen
    Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston Massachusetts 02114, USA
    Invest Ophthalmol Vis Sci 45:1117-24. 2004
    ..This study was undertaken to investigate whether deficiencies of these factors altered developmental and inflammation-induced angiogenesis in the cornea and developmental angiogenesis of the iris of mice...
  69. ncbi Reduced expression of thrombospondins and craniofacial dysmorphism in mice overexpressing Fra1
    Toru Nishiwaki
    Department of Orthopaedics, School of Medicine, Keio University, Tokyo, Japan
    J Bone Miner Res 21:596-604. 2006
    ..Fra1 Tg and non-osteosclerotic Thbs1-/-Thbs2-/- mice share an edge-to-edge bite. Therefore, reduced expression of thrombospondins may contribute to craniofacial dysmorphism independently of osteosclerosis...
  70. ncbi Thrombospondins are astrocyte-secreted proteins that promote CNS synaptogenesis
    Karen S Christopherson
    Department of Neurobiology, Stanford University School of Medicine, Stanford, California 94305, USA
    Cell 120:421-33. 2005
    ....
  71. ncbi Thrombospondin 2 deficiency in pregnant mice results in premature softening of the uterine cervix
    Robert Kokenyesi
    Department of Obstetrics, Gynecology, and Women s Health, Saint Louis University, St Louis, Missouri 63117, USA
    Biol Reprod 70:385-90. 2004
    ..Thus, TSP2-null mice provide an animal model to assist in the understanding of the molecular basis of spontaneous, premature softening of the uterine cervix...
  72. pmc Thrombospondin-1 inhibits VEGF levels in the ovary directly by binding and internalization via the low density lipoprotein receptor-related protein-1 (LRP-1)
    James Greenaway
    Department of Biomedical Sciences, University of Guelph, Guelph, Ontario, Canada
    J Cell Physiol 210:807-18. 2007
    ..In conclusion, we demonstrate a direct inhibitory effect of TSP-1 on VEGF in the ovary. TSP-1's regulation of VEGF appears to be an important mediator of ovarian angiogenesis and follicle development...
  73. ncbi Oncogenes and Angiogenesis: down-regulation of thrombospondin-1 in normal fibroblasts exposed to factors from cancer cells harboring mutant ras
    Wojciech Kalas
    Henderson Research Centre, McMaster University, Hamilton, Ontario, Canada
    Cancer Res 65:8878-86. 2005
    ..Rather, mutant ras, through a different signaling mechanism, may modulate the properties of the adjacent normal stroma, thus eliciting a proangiogenic field effect...
  74. pmc Akt1 regulates pathological angiogenesis, vascular maturation and permeability in vivo
    Juhua Chen
    Department of Molecular Cardiology, Joseph J Jacobs Center for Thrombosis and Vascular Biology, NB50, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, Ohio 44195, USA
    Nat Med 11:1188-96. 2005
    ..Re-expression of TSP-1 and TSP-2 in mice transplanted with wild-type bone marrow corrected the angiogenic abnormalities in Akt1(-/-) mice. These findings establish a crucial role of an Akt-thrombospondin axis in angiogenesis...
  75. ncbi Mice lacking thrombospondin 2 show an atypical pattern of endocortical and periosteal bone formation in response to mechanical loading
    Kurt D Hankenson
    Department of Orthopaedic Surgery, and Unit for Laboratory Animal Medicine, University of Michigan, G161 400 N Ingalls Bldg, Ann Arbor, MI 48109, USA
    Bone 38:310-6. 2006
    ....
  76. pmc Mice with a disruption of the thrombospondin 3 gene differ in geometric and biomechanical properties of bone and have accelerated development of the femoral head
    Kurt D Hankenson
    Department of Orthopaedic Surgery, and Unit for Laboratory Animal Medicine, University of Michigan, Ann Arbor, Michigan 48109, USA
    Mol Cell Biol 25:5599-606. 2005
    ..These results provide evidence for a role for TSP3 in the regulation of skeletal maturation in mice...
  77. ncbi Glycosylation mediates up-regulation of a potent antiangiogenic and proatherogenic protein, thrombospondin-1, by glucose in vascular smooth muscle cells
    Priya Raman
    Department of Molecular Cardiology, Joseph J Jacobs Center for Thrombosis and Vascular Biology, Cleveland Clinic, Cleveland, Ohio 44195, USA
    J Biol Chem 282:5704-14. 2007
    ..Our results suggest that the hexosamine pathway and intracellular glycosylation may control important steps in initiation and development of atherosclerotic lesions...
  78. ncbi Restoration of thrombospondin 1 expression in tumor cells harbouring mutant ras oncogene by treatment with low doses of doxycycline
    Wojciech Kalas
    Henderson Research Centre, Experimental Thrombosis Research, McMaster University, Hamilton, Ont, Canada L8V 1C3
    Biochem Biophys Res Commun 310:109-14. 2003
    ..Disruption of both components may be necessary for the 'rescue' of TSP-1 expression in ras-driven cancers...

Research Grants18

  1. EXTRACELLULAR MATRIX IN MICE LACKING THROMBOSPONDIN 2
    Paul Bornstein; Fiscal Year: 2005
    ..These experiments have the potential to develop the means to improve wound healing, and the performance of implanted biosensors and delivery devices, in human subjects. ..
  2. EXTRACELLULAR MATRIX IN MICE LACKING THROMBOSPONDIN 2
    Paul Bornstein; Fiscal Year: 2004
    ..These experiments have the potential to develop the means to improve wound healing, and the performance of implanted biosensors and delivery devices, in human subjects. ..
  3. BIOGENESIS AND FUNCTION OF CONNECTIVE TISSUE PROTEINS
    Paul Bornstein; Fiscal Year: 2004
    ..It is anticipated that some of the mutations created in mice will generate useful models for human disorders of these tissues, specifically some of the Ehlers-Danlos syndromes and osteoporosis. ..
  4. EXTRACELLULAR MATRIX IN MICE LACKING THROMBOSPONDIN 2
    Paul Bornstein; Fiscal Year: 2003
    ..These experiments have the potential to develop the means to improve wound healing, and the performance of implanted biosensors and delivery devices, in human subjects. ..
  5. BIOGENESIS AND FUNCTION OF CONNECTIVE TISSUE PROTEINS
    Paul Bornstein; Fiscal Year: 2003
    ..It is anticipated that some of the mutations created in mice will generate useful models for human disorders of these tissues, specifically some of the Ehlers-Danlos syndromes and osteoporosis. ..
  6. BIOGENESIS AND FUNCTION OF CONNECTIVE TISSUE PROTEINS
    Paul Bornstein; Fiscal Year: 2002
    ..It is anticipated that some of the mutations created in mice will generate useful models for human disorders of these tissues, specifically some of the Ehlers-Danlos syndromes and osteoporosis. ..
  7. EXTRACELLULAR MATRIX IN MICE LACKING THROMBOSPONDIN 2
    Paul Bornstein; Fiscal Year: 2002
    ..These experiments have the potential to develop the means to improve wound healing, and the performance of implanted biosensors and delivery devices, in human subjects. ..
  8. EXTRACELLULAR MATRIX IN MICE LACKING THROMBOSPONDIN 2
    Paul Bornstein; Fiscal Year: 2001
    ..Moreover, the elucidation of the role of TSP2 in these diverse processes is likely to reveal previously unsuspected functions for TSP2 and related proteins in connective tissue. ..
  9. BIOGENESIS AND FUNCTION OF CONNECTIVE TISSUE PROTEINS
    Paul Bornstein; Fiscal Year: 2001
    ..It is anticipated that some of the mutations created in mice will generate useful models for human disorders of these tissues, specifically some of the Ehlers-Danlos syndromes and osteoporosis. ..
  10. EXTRACELLULAR MATRIX IN MICE LACKING THROMBOSPONDIN 2
    Paul Bornstein; Fiscal Year: 2000
    ..Moreover, the elucidation of the role of TSP2 in these diverse processes is likely to reveal previously unsuspected functions for TSP2 and related proteins in connective tissue. ..
  11. BIOGENESIS AND FUNCTION OF CONNECTIVE TISSUE PROTEINS
    Paul Bornstein; Fiscal Year: 2000
    ..It is anticipated that some of the mutations created in mice will generate useful models for human disorders of these tissues, specifically some of the Ehlers-Danlos syndromes and osteoporosis. ..
  12. EXTRACELLULAR MATRIX IN MICE LACKING THROMBOSPONDIN 2
    Paul Bornstein; Fiscal Year: 1999
    ..Moreover, the elucidation of the role of TSP2 in these diverse processes is likely to reveal previously unsuspected functions for TSP2 and related proteins in connective tissue. ..
  13. EXTRACELLULAR MATRIX IN MICE LACKING THROMBOSPONDIN 2
    Paul Bornstein; Fiscal Year: 2006
    ..These experiments have the potential to develop the means to improve wound healing, and the performance of implanted biosensors and delivery devices, in human subjects. ..