Research Topics
| Julian BorejdoSummaryAffiliation: University of North Texas Country: USA Publications
Research Grants
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Detail Information
Publications
Rotation of actin monomers during isometric contraction of skeletal muscleJulian Borejdo
University of North Texas Health Science Center, Department of Molecular Biology and Immunology, Fort Worth, Texas 76107, USA
J Biomed Opt 12:014013. 2007..The root mean-squared anisotropy fluctuations are greater in isometrically contracting than in rigor myofibrils. The results support the view that during isometric contraction, cross-bridges undergo a mechanical cycle...
Single molecule detection approach to muscle study: kinetics of a single cross-bridge during contraction of muscleJulian Borejdo
Department of Molecular Biology and Immunology, Center for Commercialization of Fluorescence Technologies, University of North Texas Health Science Center, Fort Worth, TX, USA
Methods Mol Biol 875:311-34. 2012..5 and 5.8 s, respectively. These findings suggest that alterations in myosin cross-bridge kinetics associated with D166V mutation of RLC ultimately affect the ability of a heart to efficiently pump the blood...
Regulatory and essential light chains of myosin rotate equally during contraction of skeletal muscleJulian Borejdo
Department of Molecular Biology and Immunology, University of North Texas, Fort Worth, Texas 76107 2699, USA
Biophys J 82:3150-9. 2002..These results suggest that the interactions of LC1 do not inhibit the angular motion of the proximal end of the regulatory domain and that the whole domain rotates as a rigid body...- Reduction of photobleaching and photodamage in single molecule detection: observing single actin monomer in skeletal myofibrilsJulian Borejdo
University of North Texas Health Science Center, Department of Molecular Biology and Immunology, Fort Worth, Texas 76107, USA
J Biomed Opt 13:034021. 2008..They decrease damage to cells because they enhance the electric field near the fluorophore, making it possible to illuminate samples with weaker light... - E22K mutation of RLC that causes familial hypertrophic cardiomyopathy in heterozygous mouse myocardium: effect on cross-bridge kineticsD Dumka
Univ of North Texas Health Science Center, Fort Worth, TX 76107, USA
Am J Physiol Heart Circ Physiol 291:H2098-106. 2006..The slight increase in tau(1) was probably caused by myofibrillar disarray. The decrease in tau(2) of Tg hearts was probably caused by replacement of the mouse RLC for the human isoform in the Tg mice... - Rotations of a few cross-bridges in muscle by confocal total internal reflection microscopyJ Borejdo
Department of Molecular Biology and Immunology, the University of North Texas HSC, Fort Worth, TX 76107, USA
Biochim Biophys Acta 1763:137-40. 2006..Myosin heads rotated at a rate approximately equal to the ATPase rate, suggesting that bulk ATPase of a whole muscle reflects the cycle frequency of individual heads... - Measuring rotations of a few cross-bridges in skeletal muscleJulian Borejdo
Department of Molecular Biology and Immunology, University of North Texas, Fort Worth, TX 76107, USA
Exp Biol Med (Maywood) 231:28-38. 2006..The S/N ratio was sufficiently large to make statistically significant comparisons from rigor and contracting myofibrils... - Rotation of the lever arm of Myosin in contracting skeletal muscle fiber measured by two-photon anisotropyJ Borejdo
Department of Molecular Biology and Immunology, University of North Texas, Fort Worth, Texas 76107, USA
Biophys J 87:3912-21. 2004..The results indicated that the power stroke begins approximately 170 ms after the rigor cross-bridge first binds ATP... - Changes in orientation of actin during contraction of muscleJ Borejdo
Department of Molecular Biology and Immunology, University of North Texas, Fort Worth, Texas, USA
Biophys J 86:2308-17. 2004..These results suggest that orientation changes of actin are caused by dissociation and rebinding of myosin cross-bridges, and that during contraction, nucleotide does not dissociate from actin... - Orientational changes of crossbridges during single turnover of ATPJ Borejdo
Department of Molecular Biology and Immunology, University of North Texas, Fort Worth, Texas 76107 2699, USA
Biophys J 84:2450-9. 2003..A model was constructed that quantified this effect. After accounting for the slowdown, the halftimes of dissociation and attachment were 34 and 200 ms, respectively... - Kinetics of a single cross-bridge in familial hypertrophic cardiomyopathy heart muscle measured by reverse Kretschmann fluorescencePrasad Mettikolla
University of North Texas Health Science Center, Department of Molecular Biology and Immunology, Fort Worth, Texas 76107, USA
J Biomed Opt 15:017011. 2010..These results are consistent with the fact that mutated heart muscle myosin translates actin faster in in vitro motility assays... - Metal-enhanced immunoassaysIgnacy Gryczynski
Center for Commercialization of Fluorescence Technologies, University of North Texas Health Science Center, Fort Worth, TX, USA
Methods Mol Biol 875:217-29. 2012..The SACS surfaces show extraordinary fluorescence enhancements: over 100-folds in hot spots. We applied these surfaces for enhanced Alexa488 model immunoassay... - Molecular fluorescence enhancement on fractal-like structuresRafal Luchowski
Center for Commercialization of Fluorescence Technologies, UNTHSC, Fort Worth, Texas 76107, USA
Appl Spectrosc 64:578-83. 2010..We therefore believe that silver fractal-like nanostructures can be used successfully in ultrasensitive assays and fluorophore trace detection... - Decreasing photobleaching by silver nanoparticles on metal surfaces: application to muscle myofibrilsPriya Muthu
University of North Texas Health Science Center, Department of Molecular Biology and Immunology, Center for Commercialization of Fluorescent Technology, Fort Worth, Texas 76107, USA
J Biomed Opt 13:014023. 2008.... - Polarized fluorescent nanospheresRafal Luchowski
Department of Molecular Biology and Immunology, University of North Texas Health Science Center, Center for Commercialization of Fluorescence Technologies, 3500 Camp Bowie Boulevard, TX, Fort Worth, 76107, USA
Opt Express 18:4289-99. 2010..A progressive decrease of absorption was observed. Next, photophysical properties of photobleached and not bleached films dissolved in water were compared... - Fluorescence instrument response standards in two-photon time-resolved spectroscopyRafal Luchowski
Center for Commercialization of Fluorescence Technologies, Dept of Molecular Biology and Immunology, UNTHSC, Fort Worth, Texas 76107, USA
Appl Spectrosc 64:918-22. 2010..We believe that picosecond lifetime standards for two-photon excitation will find broad applications in multiphoton spectroscopy and in fluorescence lifetime imaging microscopy (FLIM)... - Single actomyosin motor interactions in skeletal muscleZeno Földes-Papp
Department of Molecular Biology and Immunology, University of North Texas Health Science Center, 3500 Camp Bowie Boulevard, Fort Worth, TX 76107, USA
Biochim Biophys Acta 1813:858-66. 2011..Clusters of polarized photons indicated an anisotropy decay of single actomyosin motors of ~9s during muscle contraction. This article is part of a Special Issue entitled: 11th European Symposium on Calcium... - Fluorescence polarization standard for near infrared spectroscopy and microscopyRafal Luchowski
Center for Commercialization of Fluorescence Technologies CCFT, Department of Molecular Biology and Immunology, University of North Texas Health Science Center, Fort Worth, TX 76107, USA
Appl Opt 47:6257-65. 2008.... - Single molecule studies of multiple-fluorophore labeled antibodies. Effect of homo-FRET on the number of photons available before photobleachingRafal Luchowski
Center for Commercialization of Fluorescence Technologies, Department of Cell Biology and Genetics, University of North Texas Health Science Center, Fort Worth, TX 76106, USA
Curr Pharm Biotechnol 9:411-20. 2008..Extending the observation time will be crucial for developing immunoassays based on single-antibody... - Lifetime-based sensing of the hyaluronidase using fluorescein labeled hyaluronic acidRafal Fudala
Department of Molecular Biology and Immunology, Center for Commercialization of Fluorescence Technologies, University of North Texas Health Science Center, Fort Worth, TX 76107, USA
J Photochem Photobiol B 106:69-73. 2012..We demonstrate that the calibration of the sensing response is the same for the average lifetime as for a single exponential decay approximation, which significantly simplifies the analysis of the sensing measurements... - Fluorescence detection of MMP-9. I. MMP-9 selectively cleaves Lys-Gly-Pro-Arg-Ser-Leu-Ser-Gly-Lys peptideRafal Fudala
Center of Commercialization of Fluorescence Technologies, Department of Molecular Biology and Immunology, UNTHSC, Fort Worth, TX 76107, USA
Curr Pharm Biotechnol 12:834-8. 2011..The control MMP-2 enzyme does not cause any changes in either 5-FAM or Cy5 fluorescence. We believe that our observation will help in early detection of elevated MMP-9 levels under disease conditions... - Instrument response standard in time-resolved fluorescenceRafal Luchowski
Department of Molecular Biology and Immunology, Center for Commercialization of Fluorescence Technologies, UNTHSC, Fort Worth, Texas 76107, USA
Rev Sci Instrum 80:033109. 2009..We demonstrate that the fluorescence decay of LDS 798 is a better approximation of IRF, in particular, for avalanche photodiodes used in the near infrared spectral region... - Elimination of autofluorescence background from fluorescence tissue images by use of time-gated detection and the AzaDiOxaTriAngulenium (ADOTA) fluorophoreRyan M Rich
Department of Molecular Biology and Immunology, Center for Commercialization of Fluorescence Technologies, University of North Texas Health Science Center, Fort Worth, TX, 76107, USA
Anal Bioanal Chem 405:2065-75. 2013..This time-gating eliminates over 96 % of autofluorescence. Analysis using a variable time-gate may enable quantitative determination of the bound probe without the contributions from the background... - Detection of hyaluronidase activity using fluorescein labeled hyaluronic acid and Fluorescence Correlation SpectroscopyRyan M Rich
Department of Molecular Biology and Immunology, Center for Commercialization of Fluorescence Technologies, University of North Texas Health Science Center, Fort Worth, TX 76107, USA
J Photochem Photobiol B 116:7-12. 2012..The method we present provides a sensitive measure of hyaluronidase activity and requires extremely small amounts of the HA substrate... - Imaging exocytosis of ATP-containing vesicles with TIRF microscopy in lung epithelial A549 cellsIrina Akopova
Center for Commercialization of Fluorescence Technologies, Department of Molecular Biology and Immunology, University of North Texas, Fort Worth, TX, USA
Purinergic Signal 8:59-70. 2012..Collectively, our data demonstrate that regulated exocytosis of ATP-loaded vesicles likely constitutes a major pathway of hypotonic stress-induced ATP secretion from A549 cells... - In situ single-molecule imaging with attoliter detection using objective total internal reflection confocal microscopyThomas P Burghardt
Department of Physiology and Biomedical Imaging, Mayo Clinic, Rochester, Minnesota 55905, USA
Biochemistry 45:4058-68. 2006..Muscle tissue contains myosin at a approximately 120 microM concentration. Evidence is provided that >75% of the bleachable fluorescence detected with fTIRM is emitted by five chromophore-labeled myosins in a muscle fiber...
Research Grants
- IMAGING MELANIN IN MELANOCYTESJulian Borejdo; Fiscal Year: 2003..The modifications are relatively simple, inexpensive and not tied to a specific brand of instrument. It is expected that the method will find application in a small diagnostic laboratory. ..
- Improved Confocal Microscope: Detection & FunctionalityJulian Borejdo; Fiscal Year: 2005..To facilitate a future commercialization, a microscope maintenance/modification company that is interested in distribution of the product version of the modification package has been identified. ..
- Rotation of Single Cross-Bridge in Skeletal Muscle FiberJulian Borejdo; Fiscal Year: 2007..This is expected to provide definitive answers about the mechanism of contraction of skeletal muscle. ..
- Single Cross-Bridge Kinetics in Transgenic Mouse Hearts Expressing FHC MutationsDanuta Szczesna Cordary; Fiscal Year: 2010....