Karol Bomsztyk

Summary

Affiliation: University of Washington
Country: USA

Publications

  1. pmc Fast chromatin immunoprecipitation assay
    Joel D Nelson
    Molecular and Cellular Biology Program, University of Washington, Seattle, WA 98109, USA
    Nucleic Acids Res 34:e2. 2006
  2. pmc Microplate-based chromatin immunoprecipitation method, Matrix ChIP: a platform to study signaling of complex genomic events
    Steve Flanagin
    UW Medicine Lake Union, Department of Medicine, University of Washington, Seattle, WA 98109, USA
    Nucleic Acids Res 36:e17. 2008
  3. pmc Direct recruitment of insulin receptor and ERK signaling cascade to insulin-inducible gene loci
    Joel D Nelson
    University of Washington, Seattle, USA
    Diabetes 60:127-37. 2011
  4. pmc Synchronous recruitment of epigenetic modifiers to endotoxin synergistically activated Tnf-α gene in acute kidney injury
    Karol Bomsztyk
    Department of Medicine, University of Washington, Seattle, Washington, USA
    PLoS ONE 8:e70322. 2013
  5. pmc Epigenetic alterations in acute kidney injury
    Karol Bomsztyk
    University of Washington Medicine Lake Union, Department of Medicine, University of Washington, Seattle, WA Electronic address
    Semin Nephrol 33:327-40. 2013
  6. pmc Allele-specific transcriptional elongation regulates monoallelic expression of the IGF2BP1 gene
    Brandon J Thomas
    Institute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, 815 Mercer St, Seattle, WA 98109, USA
    Epigenetics Chromatin 4:14. 2011
  7. pmc Halogenated imidazole derivatives block RNA polymerase II elongation along mitogen inducible genes
    Michal Mikula
    Department of Gastroenterology, Maria Sklodowska Curie Memorial Cancer Center and Institute of Oncology, Warsaw, Poland
    BMC Mol Biol 11:4. 2010
  8. ncbi request reprint Diverse molecular interactions of the hnRNP K protein
    K Bomsztyk
    Department of Medicine, University of Washington, Seattle 98195, USA
    FEBS Lett 403:113-5. 1997
  9. ncbi request reprint hnRNP K: one protein multiple processes
    Karol Bomsztyk
    Department of Medicine, University of Washington, Seattle, WA 98195, USA
    Bioessays 26:629-38. 2004
  10. ncbi request reprint Zik1, a transcriptional repressor that interacts with the heterogeneous nuclear ribonucleoprotein particle K protein
    O N Denisenko
    Department of Medicine, University of Washington, Seattle, Washington 98195, USA
    J Biol Chem 271:27701-6. 1996

Research Grants

  1. LAMININ GENE EXPRESSION IN GLOMERULAR CELLS
    Karol Bomsztyk; Fiscal Year: 1993
  2. IL 1 INDUCED KINASES & TRANSCRIPTIONAL FACTORS
    Karol Bomsztyk; Fiscal Year: 1993
  3. LAMININ GENE EXPRESSION IN GLOMERULAR CELLS
    Karol Bomsztyk; Fiscal Year: 2001
  4. INTERACTION OF K PROTEIN WITH PROTEIN KINASES
    Karol Bomsztyk; Fiscal Year: 2005
  5. INTERACTION OF K PROTEIN WITH PROTEIN KINASES
    Karol Bomsztyk; Fiscal Year: 2006
  6. LAMININ GENE EXPRESSION IN GLOMERULAR CELLS
    Karol Bomsztyk; Fiscal Year: 2007
  7. IL 1 INDUCED KINASES & TRANSCRIPTIONAL FACTORS
    Karol Bomsztyk; Fiscal Year: 1992
  8. Acute Renal Failure: An Endotoxin Hyper-Responsive State
    Richard A Zager; Fiscal Year: 2010
  9. INTERACTION OF K PROTEIN WITH PROTEIN KINASES
    Karol Bomsztyk; Fiscal Year: 1999
  10. LAMININ GENE EXPRESSION IN GLOMERULAR CELLS
    Karol Bomsztyk; Fiscal Year: 1999

Collaborators

Detail Information

Publications43

  1. pmc Fast chromatin immunoprecipitation assay
    Joel D Nelson
    Molecular and Cellular Biology Program, University of Washington, Seattle, WA 98109, USA
    Nucleic Acids Res 34:e2. 2006
    ..This method greatly facilitates the probing of chromatin changes over many time points with several antibodies in one experiment...
  2. pmc Microplate-based chromatin immunoprecipitation method, Matrix ChIP: a platform to study signaling of complex genomic events
    Steve Flanagin
    UW Medicine Lake Union, Department of Medicine, University of Washington, Seattle, WA 98109, USA
    Nucleic Acids Res 36:e17. 2008
    ..We illustrate the power of Matrix ChIP by parallel profiling 80 different chromatin and transcription time-course events along an inducible gene including transient recruitment of kinases...
  3. pmc Direct recruitment of insulin receptor and ERK signaling cascade to insulin-inducible gene loci
    Joel D Nelson
    University of Washington, Seattle, USA
    Diabetes 60:127-37. 2011
    ....
  4. pmc Synchronous recruitment of epigenetic modifiers to endotoxin synergistically activated Tnf-α gene in acute kidney injury
    Karol Bomsztyk
    Department of Medicine, University of Washington, Seattle, Washington, USA
    PLoS ONE 8:e70322. 2013
    ..This LPS hyperresponsiveness is transcriptionally mediated. The epigenetic pathways that control these responses are unknown...
  5. pmc Epigenetic alterations in acute kidney injury
    Karol Bomsztyk
    University of Washington Medicine Lake Union, Department of Medicine, University of Washington, Seattle, WA Electronic address
    Semin Nephrol 33:327-40. 2013
    ..In this article we explore what is known about the contribution of epigenetic mechanisms to the pathophysiology of AKI and how this knowledge already is guiding the development of new diagnostic tools and epigenetic therapies...
  6. pmc Allele-specific transcriptional elongation regulates monoallelic expression of the IGF2BP1 gene
    Brandon J Thomas
    Institute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, 815 Mercer St, Seattle, WA 98109, USA
    Epigenetics Chromatin 4:14. 2011
    ..abstract:..
  7. pmc Halogenated imidazole derivatives block RNA polymerase II elongation along mitogen inducible genes
    Michal Mikula
    Department of Gastroenterology, Maria Sklodowska Curie Memorial Cancer Center and Institute of Oncology, Warsaw, Poland
    BMC Mol Biol 11:4. 2010
    ..Thus, the designing and synthesis of the CK2 inhibitors has become an important pursuit in the search for cancer therapies...
  8. ncbi request reprint Diverse molecular interactions of the hnRNP K protein
    K Bomsztyk
    Department of Medicine, University of Washington, Seattle 98195, USA
    FEBS Lett 403:113-5. 1997
    ..K protein can simultaneously engage several proteins and may facilitate molecular cross-talk. Taken together these diverse interactions suggest that K protein may act as a nucleic acid-regulated docking platform...
  9. ncbi request reprint hnRNP K: one protein multiple processes
    Karol Bomsztyk
    Department of Medicine, University of Washington, Seattle, WA 98195, USA
    Bioessays 26:629-38. 2004
    ..The greater diversity of mammalian K protein interactions and function may reflect gain of novel docking sites and expansion evolutionary of gene expression networks...
  10. ncbi request reprint Zik1, a transcriptional repressor that interacts with the heterogeneous nuclear ribonucleoprotein particle K protein
    O N Denisenko
    Department of Medicine, University of Washington, Seattle, Washington 98195, USA
    J Biol Chem 271:27701-6. 1996
    ....
  11. ncbi request reprint Heterogeneous nuclear ribonucleoprotein K enhances insulin-induced expression of mitochondrial UCP2 protein
    Jerzy Ostrowski
    Department of Medicine, University of Washington, Seattle, Washington 98195, USA
    J Biol Chem 279:54599-609. 2004
    ..These results suggest the insulin stimulates translation of ucp2 mRNA in a process that involves K protein...
  12. pmc Epistatic interaction between the K-homology domain protein HEK2 and SIR1 at HMR and telomeres in yeast
    Oleg Denisenko
    Department of Medicine, Room 242, University of Washington, 815 Mercer Street, Seattle, WA 98109, USA
    J Mol Biol 375:1178-87. 2008
    ..These observations suggest that HEK2 regulates the composition of Sir complexes at HMR and telomeres...
  13. ncbi request reprint Transcription of laminin gamma1 chain gene in rat mesangial cells: constitutive and inducible RNA polymerase II recruitment and chromatin states
    Joel D Nelson
    Molecular and Cellular Biology Program, University of Washington Medicine Lake Union, Seattle, Washington 98109, USA
    Am J Physiol Renal Physiol 294:F525-33. 2008
    ....
  14. pmc Synergistic activation of the rat laminin gamma1 chain promoter by the gut-enriched Kruppel-like factor (GKLF/KLF4) and Sp1
    Yuji Higaki
    Department of Medicine, Box 356521, University of Washington, Seattle, WA 98195, USA
    Nucleic Acids Res 30:2270-9. 2002
    ..Thus, the Sp1-GKLF synergistic activation of the LAMC1 promoter may be one of the avenues for expression of laminin gamma1 chain when laminin is needed to regulate cell differentiation...
  15. pmc Renal ischemia-induced cholesterol loading: transcription factor recruitment and chromatin remodeling along the HMG CoA reductase gene
    Masayo Naito
    Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    Am J Pathol 174:54-62. 2009
    ..In conclusion, I/R activates the HMGCR gene via multiple stress-activated transcriptional and epigenetic pathways, contributing to renal cholesterol loading...
  16. pmc Transient recruitment of the hnRNP K protein to inducibly transcribed gene loci
    Jerzy Ostrowski
    Department of Medicine, University of Washington, Seattle, WA 98195, USA
    Nucleic Acids Res 31:3954-62. 2003
    ..Taken together, the results of this study suggest that the transient recruitment of K protein to serum-responsive loci depends on the inducible transcription of these immediate-early genes...
  17. ncbi request reprint Quantitative model for binary measurements of protein-protein interactions
    Yi Y Shi
    Department of Applied Mathematics, University of Washington, Seattle, Washington 98195, USA
    J Comput Biol 14:1011-23. 2007
    ..It is also shown that not only the association constants but also the protein abundance could be a factor in producing the power-law degree distribution of protein-protein interaction networks...
  18. pmc Growth and development alter susceptibility to acute renal injury
    Richard A Zager
    Department of Medicine, University of Washington, Seattle, Washington, USA
    Kidney Int 74:674-8. 2008
    ..Our study shows that the early phase of mouse growth can profoundly alter renal susceptibility to diverse forms of experimental acute renal injury...
  19. pmc BRG1 increases transcription of proinflammatory genes in renal ischemia
    Masayo Naito
    Department of Medicine, University of Washington, Seattle, WA 98109, USA
    J Am Soc Nephrol 20:1787-96. 2009
    ..In conclusion, BRG1 promotes increased transcription of TNF-alpha and MCP-1 by the proximal tubule in response to renal ischemia...
  20. pmc Microplate-based platform for combined chromatin and DNA methylation immunoprecipitation assays
    Jingjing Yu
    UW Medicine Lake Union, University of Washington, Seattle, WA 98109, USA
    BMC Mol Biol 12:49. 2011
    ..Although a critical component of chromatin structure, DNA methylation has often been studied independently of other chromatin events and transcription...
  21. pmc High ambient glucose augments angiotensin II-induced proinflammatory gene mRNA expression in human mesangial cells: effects of valsartan and simvastatin
    Masayo Naito
    UW Medicine Lake Union Research, University of Washington, Seattle, WA 98109, USA
    Am J Nephrol 30:99-111. 2009
    ..Like the ARBs, statins may also modulate inflammatory responses that are renoprotective and complement their cholesterol-lowering effects...
  22. doi request reprint Role of chromatin structure in integration of helper-dependent adenoviral vectors containing the beta-globin locus control region
    Pavel Sova
    Department of Pathology, University of Washington, Seattle, WA 98195, USA
    Hum Gene Ther 19:153-66. 2008
    ..We also provide data that suggest a role for active chromatin in AAV Rep78-mediated Ad.LCR integration. Our findings support a new strategy for achieving targeted integration through chromatin tethering of vector DNA...
  23. pmc Poly(ADP-ribose) polymerase-1 (PARP-1) contributes to the barrier function of a vertebrate chromatin insulator
    Mari Aker
    Division of Medical Genetics, University of Washington, Seattle, Washington 98195, USA
    J Biol Chem 285:37589-97. 2010
    ..Taken together, this study demonstrates that binding of PARP-1 plays a key functional role in the barrier activity of the extended cHS4 insulator core element...
  24. pmc Direct recruitment of ERK cascade components to inducible genes is regulated by heterogeneous nuclear ribonucleoprotein (hnRNP) K
    Michal Mikula
    Department of Medicine, University of Washington, Seattle, Washington 98109, USA
    J Biol Chem 286:9763-75. 2011
    ..These data suggest a model where Pol II transcription-driven recruitment of hnRNP K along the EGR-1 locus compartmentalizes activation of the ERK cascade at these genes, events that regulate synthesis of mature mRNA...
  25. ncbi request reprint Protocol for the fast chromatin immunoprecipitation (ChIP) method
    Joel D Nelson
    Molecular and Cellular Biology Program, University of Washington, Seattle, Washington 98109, USA
    Nat Protoc 1:179-85. 2006
    ..This method is particularly useful in kinetic studies where multiple samples are processed at the same time. Starting with sheared chromatin, PCR-ready DNA can be isolated from 16-24 ChIP samples in 4-6 h using the fast method...
  26. pmc Yeast hnRNP K-like genes are involved in regulation of the telomeric position effect and telomere length
    Oleg Denisenko
    Division of Nephrology, Department of Medicine, University of Washington, Seattle, Washington 98195, USA
    Mol Cell Biol 22:286-97. 2002
    ..These results identify a role for hnRNP K-like genes in the structural and functional organization of telomeric chromatin in yeast...
  27. ncbi request reprint Clustering coefficients of protein-protein interaction networks
    Gerald A Miller
    Department of Physics, University of Washington Seattle, Seattle, WA 98195, USA
    Phys Rev E Stat Nonlin Soft Matter Phys 75:051910. 2007
    ..This shows that degree distributions are not sufficient to determine the properties of PINs...
  28. doi request reprint Profiling RNA polymerase II using the fast chromatin immunoprecipitation method
    Joel Nelson
    Molecular and Cellular Biology Program, University of Washington, Seattle, WA, USA
    Methods Mol Biol 703:219-34. 2011
    ..Using Fast ChIP, 24 sheared chromatin samples can be processed to yield PCR ready DNA in 5 h...
  29. pmc Acyl-CoA synthetase 1 is induced by Gram-negative bacteria and lipopolysaccharide and is required for phospholipid turnover in stimulated macrophages
    Katya B Rubinow
    Diabetes and Obesity Center of Excellence, Department of Medicine, University of Washington, Seattle, Washington 98109, USA
    J Biol Chem 288:9957-70. 2013
    ....
  30. doi request reprint The fast chromatin immunoprecipitation method
    Joel Nelson
    Molecular and Cellular Biology Program, University of Washington, Seattle, WA, USA
    Methods Mol Biol 567:45-57. 2009
    ..Using Fast ChIP, 24 sheared chromatin samples can be processed to yield PCR-ready DNA in 5 h...
  31. pmc Free-energy distribution of binary protein-protein binding suggests cross-species interactome differences
    Yi Y Shi
    Department of Applied Mathematics, University of Washington, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 103:11527-32. 2006
    ..This result is consistent with the evolution of increased protein unfoldedness and challenges the dogma that only specific protein-protein interactions can be biologically functional...
  32. pmc Endotoxin mediates recruitment of RNA polymerase II to target genes in acute renal failure
    Masayo Naito
    Department of Medicine, University of Washington, Seattle, Washington, USA
    J Am Soc Nephrol 19:1321-30. 2008
    ..In conclusion, LPS hyperresponsiveness after ARF is likely mediated at the genomic level, possibly by H3K4m3...
  33. ncbi request reprint bcn-1 Element-dependent activation of the laminin gamma 1 chain gene by the cooperative action of transcription factor E3 (TFE3) and Smad proteins
    Yasunobu Kawata
    Department of Medicine, Division of Nephrology, University of Washington, Seattle, Washington 98195, USA
    J Biol Chem 277:11375-84. 2002
    ..Thus, the bcn-1 is a novel TFE3-dependent TGF-beta target element that regulates LAMC1 gene expression...
  34. ncbi request reprint Heterogeneous nuclear ribonucleoprotein K contributes to angiotensin II stimulation of vascular endothelial growth factor mRNA translation
    Denis Feliers
    Dept of Medicine Nephrology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Dr, San Antonio, TX, USA
    Am J Physiol Renal Physiol 293:F607-15. 2007
    ..This study shows that hnRNP K augments efficiency of VEGF mRNA translation stimulated by ANG II...
  35. ncbi request reprint Heterogeneous nuclear ribonucleoprotein K protein associates with multiple mitochondrial transcripts within the organelle
    Jerzy Ostrowski
    Department of Gastroenterology, Medical Center for Postgraduate Education, Maria Sklodowska Curie Memorial Cancer Center, and the Institute of Oncology, 02 781 Warsaw, Poland
    J Biol Chem 277:6303-10. 2002
    ..Results of this study suggest that K protein acts within functional modules that are responsible for expression of genes in mitochondria...
  36. ncbi request reprint Members of the poly (rC) binding protein family stimulate the activity of the c-myc internal ribosome entry segment in vitro and in vivo
    Joanne R Evans
    Department of Biochemistry, University of Leicester, University Road, Leicester, LE1 7RH, UK
    Oncogene 22:8012-20. 2003
    ..Interestingly, the mutated version of the c-myc IRES that is prevalent in patients with multiple myeloma bound hnRNPK more efficiently in vitro and was stimulated by hnRNPK to a greater extent in vivo...
  37. pmc c-Src-mediated phosphorylation of hnRNP K drives translational activation of specifically silenced mRNAs
    Antje Ostareck-Lederer
    European Molecular Biology Laboratory, Heidelberg, Germany
    Mol Cell Biol 22:4535-43. 2002
    ..Our results establish a novel role of c-Src kinase in translational gene regulation and reveal a mechanism by which silenced mRNAs can be translationally activated...
  38. ncbi request reprint Heterogeneous nuclear ribonucleoprotein K modulates angiotensinogen gene expression in kidney cells
    Chih Chang Wei
    Centre de Recherche, Centre Hospitalier de l Universite de Montreal Hotel Dieu, Montreal, Quebec H2W 1T8, Canada
    J Biol Chem 281:25344-55. 2006
    ..In conclusion, our experiments revealed that hnRNP K is a nuclear protein that binds to the insulin-responsive element of the rat angiotensinogen gene promoter and modulates angiotensinogen gene transcription in the kidney...
  39. ncbi request reprint Landscape of the hnRNP K protein-protein interactome
    Michał Mikula
    Department of Gastroenterology, Medical Center for Postgraduate Education and Maria Skłodowska Curie Memorial Cancer Center and Institute of Oncology, Warsaw, Poland
    Proteomics 6:2395-406. 2006
    ..These observations greatly expanded the view of the landscape of K protein-protein interaction and provide new opportunities to explore signal transduction and gene expression in several subcellular compartments...
  40. ncbi request reprint RACK1 binds to Smad3 to modulate transforming growth factor-beta1-stimulated alpha2(I) collagen transcription in renal tubular epithelial cells
    Kazuhiro Okano
    Department of Pediatrics, The Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA
    J Biol Chem 281:26196-204. 2006
    ..These results suggest that RACK1 modulates transcription of alpha2(I) collagen by TGF-beta1 through interference with Smad3 binding to the gene promoter...
  41. ncbi request reprint Cooperative binding of the hnRNP K three KH domains to mRNA targets
    Agnieszka Paziewska
    Department of Gastroenterology, Medical Center for Postgraduate Education, Maria Sklodowska Curie Memorial Cancer Center and Institute of Oncology, 02 781 Warsaw, Poland
    FEBS Lett 577:134-40. 2004
    ..The results demonstrate that in this heterologous in vivo system, the three KH domains bind RNA synergistically and that a single KH domain, in comparison, binds RNA weakly...
  42. ncbi request reprint Casein kinases phosphorylate multiple residues spanning the entire hnRNP K length
    Michał Mikula
    Institute of Oncology, 02 781 Warsaw, Poland
    Biochim Biophys Acta 1764:299-306. 2006
    ..Mass spectrometry of K protein isolated from proliferating cells and from cells under oxidative stress revealed the same pattern of phosphopeptides. The structural implications of phosphorylation are discussed...
  43. ncbi request reprint Characterization of hnRNP K protein-RNA interactions
    Karolina Klimek-Tomczak
    Department of Gastroenterology, Medical Center for Postgraduate Education at Maria Skłodowska Curie Memorial Cancer Center and Institute of Oncology, 02 781 Warsaw, Poland
    J Mol Biol 342:1131-41. 2004
    ..Genome-wide and yeast three-hybrid clone analysis revealed that these sequences are located preferentially in the 3' untranslated regions, which are known to regulate mRNA translation and processing...

Research Grants28

  1. LAMININ GENE EXPRESSION IN GLOMERULAR CELLS
    Karol Bomsztyk; Fiscal Year: 1993
    ....
  2. IL 1 INDUCED KINASES & TRANSCRIPTIONAL FACTORS
    Karol Bomsztyk; Fiscal Year: 1993
    ....
  3. LAMININ GENE EXPRESSION IN GLOMERULAR CELLS
    Karol Bomsztyk; Fiscal Year: 2001
    ..If the silencer can been narrowed down to 10-20 bp, then mutations can be made in the context of the -233/-15 fragment to abolish silencer activity. He will also test the silencer effect on heterologous promoters (SV40). ..
  4. INTERACTION OF K PROTEIN WITH PROTEIN KINASES
    Karol Bomsztyk; Fiscal Year: 2005
    ..Exploring the mechanisms and rote of K protein mitogen-induced recruitment to inducible gene loci will provide new insight into the regulation of gene expression. ..
  5. INTERACTION OF K PROTEIN WITH PROTEIN KINASES
    Karol Bomsztyk; Fiscal Year: 2006
    ..Exploring the mechanisms and rote of K protein mitogen-induced recruitment to inducible gene loci will provide new insight into the regulation of gene expression. ..
  6. LAMININ GENE EXPRESSION IN GLOMERULAR CELLS
    Karol Bomsztyk; Fiscal Year: 2007
    ..Once these are known we will define histone-modifying enzymes and factors responsible for disordered expressionof laminin chains in animal models of glomerulonephropathy. ..
  7. IL 1 INDUCED KINASES & TRANSCRIPTIONAL FACTORS
    Karol Bomsztyk; Fiscal Year: 1992
    ....
  8. Acute Renal Failure: An Endotoxin Hyper-Responsive State
    Richard A Zager; Fiscal Year: 2010
    ..We propose to define the chromatin/transcriptional mechanisms for these renal responses with the goal to develop preventive treatments in the future. ..
  9. INTERACTION OF K PROTEIN WITH PROTEIN KINASES
    Karol Bomsztyk; Fiscal Year: 1999
    ..This project will provide new insight into how the function of K protein is regulated by IL-1-induced phosphorylation, and therefore will contribute to the understanding of the action of this key cytokine. ..
  10. LAMININ GENE EXPRESSION IN GLOMERULAR CELLS
    Karol Bomsztyk; Fiscal Year: 1999
    ..If the silencer can been narrowed down to 10-20 bp, then mutations can be made in the context of the -233/-15 fragment to abolish silencer activity. He will also test the silencer effect on heterologous promoters (SV40). ..
  11. INTERACTION OF K PROTEIN WITH PROTEIN KINASES
    Karol Bomsztyk; Fiscal Year: 2007
    ..Exploring the mechanisms and rote of K protein mitogen-induced recruitment to inducible gene loci will provide new insight into the regulation of gene expression. ..
  12. INTERACTION OF K PROTEIN WITH PROTEIN KINASES
    Karol Bomsztyk; Fiscal Year: 2004
    ..Exploring the mechanisms and rote of K protein mitogen-induced recruitment to inducible gene loci will provide new insight into the regulation of gene expression. ..
  13. LAMININ GENE EXPRESSION IN GLOMERULAR CELLS
    Karol Bomsztyk; Fiscal Year: 2002
    ..If the silencer can been narrowed down to 10-20 bp, then mutations can be made in the context of the -233/-15 fragment to abolish silencer activity. He will also test the silencer effect on heterologous promoters (SV40). ..
  14. LAMININ GENE EXPRESSION IN GLOMERULAR CELLS
    Karol Bomsztyk; Fiscal Year: 2000
    ..If the silencer can been narrowed down to 10-20 bp, then mutations can be made in the context of the -233/-15 fragment to abolish silencer activity. He will also test the silencer effect on heterologous promoters (SV40). ..
  15. IL 1 INDUCED KINASES & TRANSCRIPTIONAL FACTORS
    Karol Bomsztyk; Fiscal Year: 1991
    ....