T Blumenthal

Summary

Affiliation: University of Colorado Health Sciences Center
Country: USA

Publications

  1. ncbi request reprint Gene clusters and polycistronic transcription in eukaryotes
    T Blumenthal
    Department of Biochemistry and Molecular Genetics, University of Colorado Health Sciences Center, Denver 80262, USA
    Bioessays 20:480-7. 1998
  2. ncbi request reprint Caenorhabditis elegans operons: form and function
    Thomas Blumenthal
    Department of Biochemistry and Molecular Genetics, Box B 121, University of Colorado School of Medicine, Denver, Colorado 80262, USA
    Nat Rev Genet 4:112-20. 2003
  3. ncbi request reprint A global analysis of Caenorhabditis elegans operons
    Thomas Blumenthal
    Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Box B121, 4200 E 9th Avenue, Denver, Colorado 80262, USA
    Nature 417:851-4. 2002
  4. ncbi request reprint Operons in eukaryotes
    Thomas Blumenthal
    Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine at Fitzsimons, Mail Stop 8101, PO Box 6511, Aurora, CO 80045, USA
    Brief Funct Genomic Proteomic 3:199-211. 2004
  5. pmc Interplay between AAUAAA and the trans-splice site in processing of a Caenorhabditis elegans operon pre-mRNA
    Y Liu
    Department of Biochemistry and Molecular Genetics, University of Colorado Health Sciences Center, Denver 80262, USA
    RNA 7:176-81. 2001
  6. pmc Intercistronic region required for polycistronic pre-mRNA processing in Caenorhabditis elegans
    T Huang
    Department of Biochemistry and Molecular Genetics, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
    Mol Cell Biol 21:1111-20. 2001
  7. pmc A complex containing CstF-64 and the SL2 snRNP connects mRNA 3' end formation and trans-splicing in C. elegans operons
    D Evans
    Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Denver, CO 80262, USA
    Genes Dev 15:2562-71. 2001
  8. ncbi request reprint Both subunits of U2AF recognize the 3' splice site in Caenorhabditis elegans
    D A Zorio
    Department of Biochemistry and Molecular Genetics, University of Colorado Health Sciences Center, UCHSC, Denver 80262, USA
    Nature 402:835-8. 1999
  9. pmc C. elegans sequences that control trans-splicing and operon pre-mRNA processing
    Joel H Graber
    The Jackson Laboratory, Bar Harbor, ME 04609, USA
    RNA 13:1409-26. 2007
  10. pmc Functional redundancy of worm spliceosomal proteins U1A and U2B''
    Tassa Saldi
    Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora, CO 80045, USA
    Proc Natl Acad Sci U S A 104:9753-7. 2007

Research Grants

  1. Trans-splicing in C. elegans
    Thomas Blumenthal; Fiscal Year: 2007

Collaborators

Detail Information

Publications18

  1. ncbi request reprint Gene clusters and polycistronic transcription in eukaryotes
    T Blumenthal
    Department of Biochemistry and Molecular Genetics, University of Colorado Health Sciences Center, Denver 80262, USA
    Bioessays 20:480-7. 1998
    ..This includes cases in which a polycistronic pre-mRNA is processed to make monocistronic mRNAs in nematodes, as well as isolated examples of polycistronic mRNAs found in mammals, flies, and perhaps plants...
  2. ncbi request reprint Caenorhabditis elegans operons: form and function
    Thomas Blumenthal
    Department of Biochemistry and Molecular Genetics, Box B 121, University of Colorado School of Medicine, Denver, Colorado 80262, USA
    Nat Rev Genet 4:112-20. 2003
  3. ncbi request reprint A global analysis of Caenorhabditis elegans operons
    Thomas Blumenthal
    Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Box B121, 4200 E 9th Avenue, Denver, Colorado 80262, USA
    Nature 417:851-4. 2002
    ..elegans genes. Numerous examples of co-transcription of genes encoding functionally related proteins are evident. Inspection of the operon list should reveal previously unknown functional relationships...
  4. ncbi request reprint Operons in eukaryotes
    Thomas Blumenthal
    Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine at Fitzsimons, Mail Stop 8101, PO Box 6511, Aurora, CO 80045, USA
    Brief Funct Genomic Proteomic 3:199-211. 2004
    ..Like bacterial operons, eukaryotic operons often result in co-expression of functionally related proteins...
  5. pmc Interplay between AAUAAA and the trans-splice site in processing of a Caenorhabditis elegans operon pre-mRNA
    Y Liu
    Department of Biochemistry and Molecular Genetics, University of Colorado Health Sciences Center, Denver 80262, USA
    RNA 7:176-81. 2001
    ..elegans; (2) the AAUAAA is important in determining SL2 trans-splicing events more than 100 bp downstream; and (3) in some circumstances, 3' end formation may occur by a trans-splicing-dependent mechanism...
  6. pmc Intercistronic region required for polycistronic pre-mRNA processing in Caenorhabditis elegans
    T Huang
    Department of Biochemistry and Molecular Genetics, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
    Mol Cell Biol 21:1111-20. 2001
    ..Furthermore, when the downstream gene was replaced by a gene from another organism, no loss of trans-splicing specificity was observed, suggesting that the Ur element may be the primary signal required for downstream mRNA processing...
  7. pmc A complex containing CstF-64 and the SL2 snRNP connects mRNA 3' end formation and trans-splicing in C. elegans operons
    D Evans
    Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Denver, CO 80262, USA
    Genes Dev 15:2562-71. 2001
    ..These results provide a molecular framework for the coupling of 3' end formation and trans-splicing in the processing of polycistronic pre-mRNAs from C. elegans operons...
  8. ncbi request reprint Both subunits of U2AF recognize the 3' splice site in Caenorhabditis elegans
    D A Zorio
    Department of Biochemistry and Molecular Genetics, University of Colorado Health Sciences Center, UCHSC, Denver 80262, USA
    Nature 402:835-8. 1999
    ..elegans has U2AF, 12). Here we show that both U2AF subunits crosslink to the 3' splice site. Our results suggest that the U2AF65-U2AF35 complex identifies the U4CAG/R, with U2AF35 being responsible for recognition of the canonical AG...
  9. pmc C. elegans sequences that control trans-splicing and operon pre-mRNA processing
    Joel H Graber
    The Jackson Laboratory, Bar Harbor, ME 04609, USA
    RNA 13:1409-26. 2007
    ....
  10. pmc Functional redundancy of worm spliceosomal proteins U1A and U2B''
    Tassa Saldi
    Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora, CO 80045, USA
    Proc Natl Acad Sci U S A 104:9753-7. 2007
    ..Redundancy results from the fact that one protein can substitute for the other, even though it normally does not...
  11. pmc A novel family of C. elegans snRNPs contains proteins associated with trans-splicing
    Margaret MacMorris
    Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora, CO 80045, USA
    RNA 13:511-20. 2007
    ..These phenotypic relationships, along with the association of SL26p with SL75p, imply that, like the SL1 RNA/Sm/SL75p/SL21p complex, the Sm Y/Sm/SL75p/SL26p complex is associated with trans-splicing...
  12. ncbi request reprint Crystal structure of UAP56, a DExD/H-box protein involved in pre-mRNA splicing and mRNA export
    Rui Zhao
    Department of Biochemistry and Molecular Genetics, University of Colorado Health Sciences Center, Aurora, Colorado 80045, USA
    Structure 12:1373-81. 2004
    ..The crystal structure of the N-terminal domain of UAP56 also reveals a dimer interface that is potentially important for UAP56's function...
  13. pmc The genome sequence of Caenorhabditis briggsae: a platform for comparative genomics
    Lincoln D Stein
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA
    PLoS Biol 1:E45. 2003
    ..elegans genome. Based on similarity to C. briggsae, we found strong evidence for 1,300 new C. elegans genes. In addition, comparisons of the two genomes will help to understand the evolutionary forces that mold nematode genomes...
  14. pmc UAP56 levels affect viability and mRNA export in Caenorhabditis elegans
    Margaret MacMorris
    Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Denver, Colorado 80262, USA
    RNA 9:847-57. 2003
    ..elegans, but that REF/Aly may not. It also appears that some RNA processing factors are required for viability (e.g., U2AF, PUF60, SRp54, SAP49, PRP8, U1-70K), whereas others are not (e.g., U2A', CstF50)...
  15. pmc An uncapped RNA suggests a model for Caenorhabditis elegans polycistronic pre-mRNA processing
    Yingmiao Liu
    Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Denver, Colorado 80262, USA
    RNA 9:677-87. 2003
    ..Our results also demonstrate that MS2 coat protein can be used as an in vivo block to an exonuclease, which should have utility in mRNA stability studies...
  16. pmc Coexpression of neighboring genes in Caenorhabditis elegans is mostly due to operons and duplicate genes
    Martin J Lercher
    Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK
    Genome Res 13:238-43. 2003
    ..The central importance of tandem duplicate genes in these patterns renders the worm genome different from both yeast and human...
  17. pmc U2AF binding selects for the high conservation of the C. elegans 3' splice site
    Courtney Hollins
    Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Mail Stop 8101, P O Box 6511, Aurora, CO 80045, USA
    RNA 11:248-53. 2005
    ..Finally, the C in the consensus sequence must remain adjacent to the AG/R rather than to the stretch of U's, suggesting this C is recognized by U2AF35...
  18. doi request reprint Medicine. The cart before the horse
    Janet D Rowley
    Department of Medicine, University of Chicago, 5841 South Maryland Avenue, MC 2115, Chicago, IL 60637, USA
    Science 321:1302-4. 2008

Research Grants1

  1. Trans-splicing in C. elegans
    Thomas Blumenthal; Fiscal Year: 2007
    ..We will determine what functions these proteins have in 3' end formation. These studies should provide important new insight into how 3' end cleavage can occur without accompanying transcription termination. ..