Research TopicsGenomes and Genes | PAUL L BLOUNTSummaryAffiliation: University of Texas Southwestern Medical Center Country: USA Publications
Research Grants
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Detail Information
Publications
Molecular mechanisms of mechanosensation: big lessons from small cellsPaul Blount
Department of Physiology, University of Texas, Southwestern Medical Center, Dallas, TX 75390, USA
Neuron 37:731-4. 2003..These studies give clues to general principles underlying the ability of a membrane protein to sense and respond to perturbations of its lipid environment that may be conserved between bacteria and humans...
An in vivo screen reveals protein-lipid interactions crucial for gating a mechanosensitive channelIrene Iscla
Department of Physiology, UT Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd, Dallas, TX 75235, USA
FASEB J 25:694-702. 2011..Furthermore, the conservation of analogous motifs among many other channels suggests a conserved protein-lipid dynamic mechanism...- Manipulating the permeation of charged compounds through the MscL nanovalveLi Min Yang
Department of Physiology, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd, Dallas, TX 75390, USA
FASEB J 25:428-34. 2011..These effects were more drastic for larger compounds and, surprisingly, were related to the orientation of the MscL channel in the membrane... - An open-pore structure of the mechanosensitive channel MscL derived by determining transmembrane domain interactions upon gatingYuezhou Li
Department of Physiology, University of Texas, Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd, Dallas, TX 75390 9040, USA
FASEB J 23:2197-204. 2009..Together, the data strongly suggest a model for structural transitions and residue-residue proximities that occur upon MscL gating... - On the structure of the N-terminal domain of the MscL channel: helical bundle or membrane interfaceIrene Iscla
Department of Physiology, University of Texas, Southwestern Medical Center at Dallas, Dallas, Texas 75390 9040, USA
Biophys J 95:2283-91. 2008..tuberculosis MscL. We propose a functional model that is consistent with the collective data... - Disulfide trapping the mechanosensitive channel MscL into a gating-transition stateIrene Iscla
Department of Physiology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9040, USA
Biophys J 92:1224-32. 2007..The data are not easily explained by current models for the smooth transition from closed-to-open states, but predict that an asymmetric movement of one or more of the subunits commonly occurs upon gating... - Mechanosensitive channel gating transitions resolved by functional changes upon pore modificationJessica L Bartlett
Department of Physiology, University of Texas Southwestern Medical Center, Dallas, Texas, USA
Biophys J 91:3684-91. 2006.... - Defining the physical gate of a mechanosensitive channel, MscL, by engineering metal-binding sitesIrene Iscla
Department of Physiology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9040, USA
Biophys J 87:3172-80. 2004.... - An in vivo assay identifies changes in residue accessibility on mechanosensitive channel gatingJessica L Bartlett
Department of Physiology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9040, USA
Proc Natl Acad Sci U S A 101:10161-5. 2004.... - Family ties of gated pores: evolution of the sensor moduleAttila Kumanovics
Center for Immunology and, Department of Physiology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9040, USA
FASEB J 16:1623-9. 2002..These data provide evidence for the modularity of the sensor, imply a model for its evolution, suggest a common origin for mechano- and voltage-sensing, and may offer a glimpse of the properties of the first sensor/channel... - Ionic regulation of MscK, a mechanosensitive channel from Escherichia coliYuezhou Li
Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9040, USA
EMBO J 21:5323-30. 2002..Increased sensitivity and the highly regulated nature of MscK suggest a more specialized physiological role than other bacterial mechanosensitive channels...
Research Grants
- MOLECULAR MECHANISMS OF MECHANOSENSITIVE CHANNEL GATINGPaul Blount; Fiscal Year: 2009..This work could also have implications in anti-bacterial drug design and the utilization of biological sensors for future technological feats, such as nanodevices for drug delivery. ..
- MOLECULAR MECHANISMS OF MECHANOSENSITIVE CHANNEL GATINGPaul Blount; Fiscal Year: 2007..This work could also have implications in anti-bacterial drug design and the utilization of biological sensors for future technological feats, such as nanodevices for drug delivery. ..
- Mechanosensory Transduction Gordon ConferencePaul Blount; Fiscal Year: 2007....
- MOLECULAR MECHANISMS OF MECHANOSENSITIVE CHANNEL GATINGPaul Blount; Fiscal Year: 2007..This work could also have implications in anti-bacterial drug design and the utilization of biological sensors for future technological feats, such as nanodevices for drug delivery. ..
- MOLECULAR MECHANISMS OF MECHANOSENSITIVE CHANNEL GATINGPaul Blount; Fiscal Year: 2004..3) Isolating and characterizing suppressor mutations of gain-of-function MscL mutants to identify pairs of residues that potentially interact in the closed, transition or open states. ..
- Using Microbial Genetics to Study Eukaryotic ChannelsPaul Blount; Fiscal Year: 2002..Finally, we will pursue the development of a system that will allow for a similar study of homologously and heterologously expressed mechano- and thermo-sensitive eukaryotic channels in yeast (S. pombe). ..
- MOLECULAR MECHANISMS OF MECHANOSENSITIVE CHANNEL GATINGPaul Blount; Fiscal Year: 2009..This work could also have implications in anti-bacterial drug design and the utilization of biological sensors for future technological feats, such as nanodevices for drug delivery. ..