PAUL L BLOUNT

Summary

Affiliation: University of Texas Southwestern Medical Center
Country: USA

Publications

  1. ncbi request reprint Molecular mechanisms of mechanosensation: big lessons from small cells
    Paul Blount
    Department of Physiology, University of Texas, Southwestern Medical Center, Dallas, TX 75390, USA
    Neuron 37:731-4. 2003
  2. pmc An in vivo screen reveals protein-lipid interactions crucial for gating a mechanosensitive channel
    Irene Iscla
    Department of Physiology, UT Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd, Dallas, TX 75235, USA
    FASEB J 25:694-702. 2011
  3. pmc Manipulating the permeation of charged compounds through the MscL nanovalve
    Li Min Yang
    Department of Physiology, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd, Dallas, TX 75390, USA
    FASEB J 25:428-34. 2011
  4. pmc An open-pore structure of the mechanosensitive channel MscL derived by determining transmembrane domain interactions upon gating
    Yuezhou Li
    Department of Physiology, University of Texas, Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd, Dallas, TX 75390 9040, USA
    FASEB J 23:2197-204. 2009
  5. pmc On the structure of the N-terminal domain of the MscL channel: helical bundle or membrane interface
    Irene Iscla
    Department of Physiology, University of Texas, Southwestern Medical Center at Dallas, Dallas, Texas 75390 9040, USA
    Biophys J 95:2283-91. 2008
  6. pmc Disulfide trapping the mechanosensitive channel MscL into a gating-transition state
    Irene Iscla
    Department of Physiology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9040, USA
    Biophys J 92:1224-32. 2007
  7. pmc Mechanosensitive channel gating transitions resolved by functional changes upon pore modification
    Jessica L Bartlett
    Department of Physiology, University of Texas Southwestern Medical Center, Dallas, Texas, USA
    Biophys J 91:3684-91. 2006
  8. pmc Defining the physical gate of a mechanosensitive channel, MscL, by engineering metal-binding sites
    Irene Iscla
    Department of Physiology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9040, USA
    Biophys J 87:3172-80. 2004
  9. pmc An in vivo assay identifies changes in residue accessibility on mechanosensitive channel gating
    Jessica L Bartlett
    Department of Physiology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9040, USA
    Proc Natl Acad Sci U S A 101:10161-5. 2004
  10. ncbi request reprint Family ties of gated pores: evolution of the sensor module
    Attila Kumanovics
    Center for Immunology and, Department of Physiology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9040, USA
    FASEB J 16:1623-9. 2002

Collaborators

  • Irene Iscla
  • Robin Wray
  • Yuezhou Li
  • Jessica L Bartlett
  • Gal Levin
  • Li Min Yang
  • Attila Kumanovics
  • Christina Eaton
  • Robert Reynolds
  • Subramanian Chandrasekaran
  • Ian R Booth
  • Paul C Moe

Detail Information

Publications11

  1. ncbi request reprint Molecular mechanisms of mechanosensation: big lessons from small cells
    Paul Blount
    Department of Physiology, University of Texas, Southwestern Medical Center, Dallas, TX 75390, USA
    Neuron 37:731-4. 2003
    ..These studies give clues to general principles underlying the ability of a membrane protein to sense and respond to perturbations of its lipid environment that may be conserved between bacteria and humans...
  2. pmc An in vivo screen reveals protein-lipid interactions crucial for gating a mechanosensitive channel
    Irene Iscla
    Department of Physiology, UT Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd, Dallas, TX 75235, USA
    FASEB J 25:694-702. 2011
    ..Furthermore, the conservation of analogous motifs among many other channels suggests a conserved protein-lipid dynamic mechanism...
  3. pmc Manipulating the permeation of charged compounds through the MscL nanovalve
    Li Min Yang
    Department of Physiology, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd, Dallas, TX 75390, USA
    FASEB J 25:428-34. 2011
    ..These effects were more drastic for larger compounds and, surprisingly, were related to the orientation of the MscL channel in the membrane...
  4. pmc An open-pore structure of the mechanosensitive channel MscL derived by determining transmembrane domain interactions upon gating
    Yuezhou Li
    Department of Physiology, University of Texas, Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd, Dallas, TX 75390 9040, USA
    FASEB J 23:2197-204. 2009
    ..Together, the data strongly suggest a model for structural transitions and residue-residue proximities that occur upon MscL gating...
  5. pmc On the structure of the N-terminal domain of the MscL channel: helical bundle or membrane interface
    Irene Iscla
    Department of Physiology, University of Texas, Southwestern Medical Center at Dallas, Dallas, Texas 75390 9040, USA
    Biophys J 95:2283-91. 2008
    ..tuberculosis MscL. We propose a functional model that is consistent with the collective data...
  6. pmc Disulfide trapping the mechanosensitive channel MscL into a gating-transition state
    Irene Iscla
    Department of Physiology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9040, USA
    Biophys J 92:1224-32. 2007
    ..The data are not easily explained by current models for the smooth transition from closed-to-open states, but predict that an asymmetric movement of one or more of the subunits commonly occurs upon gating...
  7. pmc Mechanosensitive channel gating transitions resolved by functional changes upon pore modification
    Jessica L Bartlett
    Department of Physiology, University of Texas Southwestern Medical Center, Dallas, Texas, USA
    Biophys J 91:3684-91. 2006
    ....
  8. pmc Defining the physical gate of a mechanosensitive channel, MscL, by engineering metal-binding sites
    Irene Iscla
    Department of Physiology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9040, USA
    Biophys J 87:3172-80. 2004
    ....
  9. pmc An in vivo assay identifies changes in residue accessibility on mechanosensitive channel gating
    Jessica L Bartlett
    Department of Physiology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9040, USA
    Proc Natl Acad Sci U S A 101:10161-5. 2004
    ....
  10. ncbi request reprint Family ties of gated pores: evolution of the sensor module
    Attila Kumanovics
    Center for Immunology and, Department of Physiology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9040, USA
    FASEB J 16:1623-9. 2002
    ..These data provide evidence for the modularity of the sensor, imply a model for its evolution, suggest a common origin for mechano- and voltage-sensing, and may offer a glimpse of the properties of the first sensor/channel...
  11. pmc Ionic regulation of MscK, a mechanosensitive channel from Escherichia coli
    Yuezhou Li
    Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9040, USA
    EMBO J 21:5323-30. 2002
    ..Increased sensitivity and the highly regulated nature of MscK suggest a more specialized physiological role than other bacterial mechanosensitive channels...

Research Grants12

  1. MOLECULAR MECHANISMS OF MECHANOSENSITIVE CHANNEL GATING
    Paul Blount; Fiscal Year: 2009
    ..This work could also have implications in anti-bacterial drug design and the utilization of biological sensors for future technological feats, such as nanodevices for drug delivery. ..
  2. MOLECULAR MECHANISMS OF MECHANOSENSITIVE CHANNEL GATING
    Paul Blount; Fiscal Year: 2007
    ..This work could also have implications in anti-bacterial drug design and the utilization of biological sensors for future technological feats, such as nanodevices for drug delivery. ..
  3. Mechanosensory Transduction Gordon Conference
    Paul Blount; Fiscal Year: 2007
    ....
  4. MOLECULAR MECHANISMS OF MECHANOSENSITIVE CHANNEL GATING
    Paul Blount; Fiscal Year: 2007
    ..This work could also have implications in anti-bacterial drug design and the utilization of biological sensors for future technological feats, such as nanodevices for drug delivery. ..
  5. MOLECULAR MECHANISMS OF MECHANOSENSITIVE CHANNEL GATING
    Paul Blount; Fiscal Year: 2004
    ..3) Isolating and characterizing suppressor mutations of gain-of-function MscL mutants to identify pairs of residues that potentially interact in the closed, transition or open states. ..
  6. Using Microbial Genetics to Study Eukaryotic Channels
    Paul Blount; Fiscal Year: 2002
    ..Finally, we will pursue the development of a system that will allow for a similar study of homologously and heterologously expressed mechano- and thermo-sensitive eukaryotic channels in yeast (S. pombe). ..
  7. MOLECULAR MECHANISMS OF MECHANOSENSITIVE CHANNEL GATING
    Paul Blount; Fiscal Year: 2009
    ..This work could also have implications in anti-bacterial drug design and the utilization of biological sensors for future technological feats, such as nanodevices for drug delivery. ..