P Blount

Summary

Affiliation: University of Texas Southwestern Medical Center
Country: USA

Publications

  1. ncbi Bacterial mechanosensitive channels: integrating physiology, structure and function
    P Blount
    Dept of Physiology, University of Texas Southwestern Medical Centre, Dallas, TX 75235 9040, USA
    Trends Microbiol 7:420-4. 1999
  2. ncbi A large-conductance mechanosensitive channel in E. coli encoded by mscL alone
    S I Sukharev
    Laboratory of Molecular Biology, University of Wisconsin Madison 53706
    Nature 368:265-8. 1994
  3. ncbi Mechanosensitive channels of bacteria
    P Blount
    U TX Southwestern Med Ctr, Dallas
    Methods Enzymol 294:458-82. 1999
  4. ncbi One face of a transmembrane helix is crucial in mechanosensitive channel gating
    X Ou
    Laboratory of Molecular Biology and Department of Genetics, University of Wisconsin, 1525 Linden Drive, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 95:11471-5. 1998
  5. ncbi Functional and structural conservation in the mechanosensitive channel MscL implicates elements crucial for mechanosensation
    P C Moe
    Laboratory of Molecular Biology and Department of Bacteriology, University of Wisconsin Madison, 53706, USA
    Mol Microbiol 28:583-92. 1998
  6. ncbi Helicity, membrane incorporation, orientation and thermal stability of the large conductance mechanosensitive ion channel from E. coli
    I T Arkin
    Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University, New Haven, CT 06520, USA
    Biochim Biophys Acta 1369:131-40. 1998
  7. ncbi Mutations in a bacterial mechanosensitive channel change the cellular response to osmotic stress
    P Blount
    Laboratory of Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Biol Chem 272:32150-7. 1997
  8. ncbi Mechanosensitive channels of Escherichia coli: the MscL gene, protein, and activities
    S I Sukharev
    Laboratory of Molecular Biology, University of Wisconsin, Madison 53706, USA
    Annu Rev Physiol 59:633-57. 1997
  9. ncbi Towards an understanding of the structural and functional properties of MscL, a mechanosensitive channel in bacteria
    P Blount
    Laboratory of Molecular Biology, Bock Labs, University of Wisconsin Madison 53706, USA
    Biol Cell 87:1-8. 1996
  10. ncbi Membrane topology and multimeric structure of a mechanosensitive channel protein of Escherichia coli
    P Blount
    Laboratory of Molecular Biology, University of Wisconsin Madison 53706, USA
    EMBO J 15:4798-805. 1996

Collaborators

  • B Martinac
  • C Kung
  • S I Sukharev
  • K Yoshimura
  • I T Arkin
  • P C Moe
  • X Ou
  • M Schroeder
  • A Batiza
  • R J Hoffman
  • A T Brunger
  • H R Guy
  • F R Blattner

Detail Information

Publications14

  1. ncbi Bacterial mechanosensitive channels: integrating physiology, structure and function
    P Blount
    Dept of Physiology, University of Texas Southwestern Medical Centre, Dallas, TX 75235 9040, USA
    Trends Microbiol 7:420-4. 1999
    ..Mutagenesis of one of these channels, combined with structural information derived from X-ray crystallography, has given the first clues of how a mechanosensitive channel senses and responds to membrane tension...
  2. ncbi A large-conductance mechanosensitive channel in E. coli encoded by mscL alone
    S I Sukharev
    Laboratory of Molecular Biology, University of Wisconsin Madison 53706
    Nature 368:265-8. 1994
    ..The mscL nucleotide sequence predicts a unique protein of only 136 amino acids, with a highly hydrophobic core and very different from porins or other known proteins...
  3. ncbi Mechanosensitive channels of bacteria
    P Blount
    U TX Southwestern Med Ctr, Dallas
    Methods Enzymol 294:458-82. 1999
  4. ncbi One face of a transmembrane helix is crucial in mechanosensitive channel gating
    X Ou
    Laboratory of Molecular Biology and Department of Genetics, University of Wisconsin, 1525 Linden Drive, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 95:11471-5. 1998
    ..When this domain is modeled as an alpha-helix, the most severe mutations are substitutions of smaller amino acids (three glycines and one valine) on one facet, suggesting an important role for this structure in MS channel gating...
  5. ncbi Functional and structural conservation in the mechanosensitive channel MscL implicates elements crucial for mechanosensation
    P C Moe
    Laboratory of Molecular Biology and Department of Bacteriology, University of Wisconsin Madison, 53706, USA
    Mol Microbiol 28:583-92. 1998
    ..This structural conservation is providing insight into regions of this molecule that are vital to its role as a mechanosensitive channel and may have broader implications for the understanding of other mechanosensitive systems...
  6. ncbi Helicity, membrane incorporation, orientation and thermal stability of the large conductance mechanosensitive ion channel from E. coli
    I T Arkin
    Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University, New Haven, CT 06520, USA
    Biochim Biophys Acta 1369:131-40. 1998
    ..Here, we present and discuss this initial set of structural data on this new family of ion-channel proteins...
  7. ncbi Mutations in a bacterial mechanosensitive channel change the cellular response to osmotic stress
    P Blount
    Laboratory of Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Biol Chem 272:32150-7. 1997
    ..These data are consistent with a crucial role for MscL in the adaptation to large osmotic downshock and suggest that if the normally tight regulation of MscL gating is disrupted, cell growth can be severely inhibited...
  8. ncbi Mechanosensitive channels of Escherichia coli: the MscL gene, protein, and activities
    S I Sukharev
    Laboratory of Molecular Biology, University of Wisconsin, Madison 53706, USA
    Annu Rev Physiol 59:633-57. 1997
    ..Although unique when discovered, highly conserved MscL homologues in both gram-negative and gram-positive bacteria have been found, suggesting their ubiquitous importance among bacteria...
  9. ncbi Towards an understanding of the structural and functional properties of MscL, a mechanosensitive channel in bacteria
    P Blount
    Laboratory of Molecular Biology, Bock Labs, University of Wisconsin Madison 53706, USA
    Biol Cell 87:1-8. 1996
    ..The study of the MscL protein remains, to date, one of the most viable options for understanding the structural and functional characteristics of a mechanosensitive channel...
  10. ncbi Membrane topology and multimeric structure of a mechanosensitive channel protein of Escherichia coli
    P Blount
    Laboratory of Molecular Biology, University of Wisconsin Madison 53706, USA
    EMBO J 15:4798-805. 1996
    ..Finally, cross-linking studies suggest that the functional MscL complex is a homohexamer. In summary, these data are all consistent with a protein domain assignment and topological model which we propose and discuss...
  11. ncbi Single residue substitutions that change the gating properties of a mechanosensitive channel in Escherichia coli
    P Blount
    Laboratory of Molecular Biology, University of Wisconsin, Madison 53706, USA
    Proc Natl Acad Sci U S A 93:11652-7. 1996
    ....
  12. ncbi Hydrophilicity of a single residue within MscL correlates with increased channel mechanosensitivity
    K Yoshimura
    Laboratory of Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706, USA
    Biophys J 77:1960-72. 1999
    ..These results suggest a model for gating in which Gly(22) moves from a hydrophobic, and through a hydrophilic, environment upon transition from the closed to open conformation...
  13. ncbi Mechanosensitive channels of E. coli: a genetic and molecular dissection
    P Blount
    Laboratory of Molecular Biology, University of Wisconsin, Madison 53706, USA
    Biol Bull 192:126-7. 1997
  14. ncbi MscL: a mechanosensitive channel in Escherichia coli
    S I Sukharev
    Laboratory of Molecular Biology, University of Wisconsin, Madison 53706, USA
    Soc Gen Physiol Ser 51:133-41. 1996